Life Sciences Commons^™

Eco-Friendly Nets To Improve Vegetable Production And Quality In Sub-Saharan Africa, T. Martin, S. Simon, L. Parrot, F. Assogba Komlan, F. Vidogbena, A. Adegbidi, V. Baird, M. Saidi, M. Kasina, L. A. Wasilwa, S. Subramanian, M. Ngouajio

All Peer-Reviewed Publications

Continuous coverage of vegetables with nets under different climate conditions in Benin and Kenya proved effective in protecting vegetable nurseries and main fields against key lepidopteran and dipteran pests. However, netting did not effectively protect the vegetables against small insects such as aphids and whiteflies, although a visual barrier could delay outbreaks. Hence, opening the nets during the day could be an alternative option to conserve the natural enemies for small insects. Moreover, the efficacy of the visual barrier may be complemented by a repellent compound impregnated in the net. The small-holder growers adopting the nets in Benin and Kenya …

Antagonistic Interactions Between The Africanweaver Ant Oecophylla Longinoda And The Parasitoid Anagyrus Pseudococci Potentially Limits Suppression Of The Invasive Mealybug Rastrococcus Iceryoides, Chrysantus M. Tanga, Sunday Ekesi, Prem Govender, Peterson W. Nderitu, Samira A. Mohamed

All Peer-Reviewed Publications

The ant Oecophylla longinoda Latreille forms a trophobiotic relationship with the invasive mealybug Rastrococus iceryoides Green and promotes the latter’s infestations to unacceptable levels in the presence of their natural enemies. In this regard, the antagonistic interactions between the ant and the parasitoid Anagyrus pseudococci Girault were assessed under laboratory conditions. The percentage of parasitism of R. iceryoides by A. pseudococci was significantly higher on “ant-excluded” treatments (86.6% ± 1.27%) compared to “ant-tended” treatments (51.4% ± 4.13%). The low female-biased sex-ratio observed in the “ant-tended” treatment can be attributed to ants’ interference during the oviposition phase, which disrupted parasitoids’ ability …

Vestigial Serrula In Scorpion Genera Paravaejovis, , Smeringurus And Vejovoidus (Scorpiones: Vaejovidae), Victor Fet, Michael E. Soleglad, Michael S. Brewer, David P. A. Neff, Michael L. Norton

Euscorpius

The presence of vestigial serrula on the ventral edge of the cheliceral movable finger is established for vaejovid genera Paravaejovis, Paruroctonus, Smeringurus, and Vejovoidus. Detailed descriptions and SEM images illustrating the various manifestations of this interesting cheliceral structure are provided for many species of these four genera. A brief systematic overview of this structure as it exists in Recent scorpions is also presented.

Serrula In Retrospect: A Historical Look At Scorpion Literature (Scorpiones: Orthosterni), Matthew R. Graham, Victor Fet

Euscorpius

Serrula, a small comb-like structure on the ventral aspect of cheliceral movable finger in scorpions, has often been overlooked or ignored. Scorpion genera that are currently known to possess serrula belong to six families: Chactidae, Euscorpiidae, Iuridae, Pseudochactidae, Superstitioniidae, and Vaejovidae. All relevant literature on scorpion serrula is reviewed, and serrula of representative scorpion taxa is illustrated by SEM images. We suggest that the inconsistency in reporting serrula is based on several factors (or combination thereof): (a) serrula tines can quite often be broken off partially or completely, especially in adults; (b) serrula can be vestigial and hardly visible under …

On The Poorly Known Genus Mesotityus González-Sponga, 1981 (Scorpiones: Buthidae), Luis F. De Armas, Fernando J. M. Rojas-Runjaic

Euscorpius

Examination of new available material (18 females and six males) of the rare Venezuelan scorpion Mesotityus vondangeli González-Sponga, 1981 (Buthidae) demonstrated that there are consistent taxonomic characters to consider it as a genus different from Tityus C. L. Koch, 1836 sensu stricto. Nevertheless, it shares important characters with species of the Tityus clathratus group (=subgenus Archaeotityus Lourenço, 2006). An emended diagnosis of Mesotityus is given, as well as data on natural history, meristic and morphometric variation.

From Lab To Pharmacy Shelves: The Story Of A Plant Derived Anticancer Drug, "Paclitaxel", Maira Junjua, Sana Jafar, Fazeelat Karamat, Faheem Ahmed

Journal of Bioresource Management

Paclitaxel (Taxol®) is a highly praised anticancer drug, known for its efficiency in treating different cancers. It belongs to a class of compounds called taxanes that are derived from the plants of the genus Taxus. This drug is now FDA approved, but there was a time when the name Taxol was less known to the scientific world. Taxol, being a unique molecule in its structure, properties and mechanism of action, has undergone more than 30 years of trials, and faced a lot of hurdles to leave the bench of a laboratory and reach the shelves of a pharmacy. This review …

Status And Future Management Of Grey Goral (Naemorhedus Goral Bedfordi) In Pakistan, Fakhar -I- Abbas, Afsar Mian, Tanveer Akhtar, Thomas P. Rooney

Journal of Bioresource Management

Himalayan grey goral (HGG: Naemorhedus goral bedfordi) is endemic to Himalyas and regarded as Endangered/ Threatened. Study was designed to collect information on population biology, habitat, food and behaviour of population of HGG distributed in Pakistan, trying to understand its present status and conservational potentials. Our data suggest that the population, habitat and the species has sufficient potentials for its survival in the area, if protection from human predation is afforded to the species. HGG population is isolated into 7-8 subpopulations and is facing male-biased mortality, therefore is likely to face bottleneck effects and subsequent population crash ascribed to …

Dna Protective Activity Of Peels Of Some Vegetables Wastes, Aisha Ishaq, Kainaat William

Journal of Bioresource Management

The present study was conducted by using kitchen wastes like peels of different vegetables e.g. cucumber, potato, carrot, chickpea, cauliflower and pea, to evaluate the phytochemical constituents, DNA protection ability and free radicals scavenging activity. Phytochemicals tests showed the presence of carbohydrates, amino acids, glycosides, alkaloids, tannins and steroids in all samples while phlobatannins, anthraquonines and saponins were absent. The DNA protection of samples was checked by DNA protection assay using plasmid pBR322. Cauliflower and cucumber showed best protection at each dilution. DPPH free radicals scavenging assay was used to evaluate the antioxidant activity of peel extract of vegetables. Carrot …

Taxonomic Position Of The Genus Sassanidotus Farzanpay, 1987 (Scorpiones: Buthidae), František Kovařík, Victor Fet

Euscorpius

Sassanidotus Farzanpay, 1987 is a valid genus closely related to Compsobuthus Vachon, 1949, with which it shares most characters including the shape of carinae on the carapace and mesosoma. The main difference is that Sassanidotus has only three granules located proximally to the terminal granule on the movable finger of pedipalp, whereas Compsobuthus and also Mesobuthus Vachon, 1950 have four such granules. Sassanidotus Farzanpay, 1987 includes two species, Sassanidotus zarudnyi (Birula, 1900) (type species) from Iran and Sassanidotus gracilis thus zarudnyi sarghadensis Birula, 1903, syn. n.) from Afghanistan, Iran, and Pakistan. The neotype is designated for S. zarudnyi.

Ethylene, A Hormone At The Center-Stage Of Nodulation, Frédérique C. Guinel

Biology Faculty Publications

Nodulation is the result of a beneficial interaction between legumes and rhizobia. It is a sophisticated process leading to nutrient exchange between the two types of symbionts. In this association, within a nodule, the rhizobia, using energy provided as photosynthates, fix atmospheric nitrogen and convert it to ammonium which is available to the plant. Nodulation is recognized as an essential process in nitrogen cycling and legume crops are known to enrich agricultural soils in nitrogenous compounds. Furthermore, as they are rich in nitrogen, legumes are considered important as staple foods for humans and fodder for animals. To tightly control this …

U.S. Drought Monitor, December 22, 2015, Richard R. Heim Jr.

United States Agricultural Commodities in Drought Archive

Drought map of the United States for December 22, 2015 (12/22/15) plus: United States crop areas experiencing drought (map), an approximate percentage of crop located in drought by state (bar graph), and the percentage of crop area located in drought during the past 52 weeks (line graph) for corn, soybeans, hay, cattle, and winter wheat.

Reliability And Validity Of Neurobehavioral Function On The Psychology Experimental Building Language Test Battery In Young Adults, Brian J. Piper, Shane Mueller, Alexander R. Geerken, Kyle L. Dixon, Gregory Kroliczak, Reid H. Olsen, Jeremy K. Miller

Michigan Tech Publications, Part 1

Background. The Psychology Experiment Building Language (PEBL) software consists of over one-hundred computerized tests based on classic and novel cognitive neuropsychology and behavioral neurology measures. Although the PEBL tests are becoming more widely utilized, there is currently very limited information about the psychometric properties of these measures.

Methods. Study I examined inter-relationships among nine PEBL tests including indices of motor-function (Pursuit Rotor and Dexterity), attention (Test of Attentional Vigilance and Time-Wall), working memory (Digit Span Forward), and executive-function (PEBL Trail Making Test, Berg/Wisconsin Card Sorting Test, Iowa Gambling Test, and Mental Rotation) in a normative sample (N = 189, …

Pvt1 Exon 9: A Potential Biomarker Of Aggressive Prostate Cancer?, Adeodat Ilboudo, Jyoti Chouhan, Brian K. Mcneil, Joseph R. Osborne, Olorunseun O. Ogunwobi

Publications and Research

Prostate cancer (PCa) is the most commonly diagnosed cancer as well as the greatest source of cancer-related mortality in males of African ancestry (MoAA). Interestingly, this has been shown to be associated with single nucleotide polymorphisms around regions 2 and 3 of the 8q24 human chromosomal region. The non-protein coding gene locus Plasmacytoma Variant Translocation 1 (PVT1) is located at 8q24 and is overexpressed in PCa and, therefore, is also a candidate biomarker to explain the well-known disparity in this group. PVT1 has at least 12 exons that make separate transcripts which may have different functions, all of which are …

Period-1 Encodes An Atp-Dependent Rna Helicase That Influences Nutritional Compensation Of The Neurospora Circadian Clock, Jillian M. Emerson, Bradley M. Bartholomai, Carol S. Ringelberg, Scott E. Baker, Jennifer Loros, Jay Dunlap

Dartmouth Scholarship

Mutants in the period-1 (prd-1) gene, characterized by a recessive allele, display a reduced growth rate and period lengthening of the developmental cycle controlled by the circadian clock. We refined the genetic location of prd-1 and used whole genome sequencing to find the mutation defining it, confirming the identity of prd-1 by rescuing the mutant circadian phenotype via transformation. PRD-1 is an RNA helicase whose orthologs, DDX5 [DEAD (Asp-Glu-Ala-Asp) Box Helicase 5] and DDX17 in humans and DBP2 (Dead Box Protein 2) in yeast, are implicated in various processes, including transcriptional regulation, elongation, and termination, ribosome biogenesis, and mRNA decay. …

Fungal Melanin: What Do We Know About Structure?, Joshua D. Nosanchuk, Ruth E. Stark, Arturo Casadevall

Publications and Research

The production of melanin significantly enhances the virulence of many important human pathogenic fungi. Despite fungal melanin’s importance in human disease, as well as melanin’s contribution to the ability of fungi to survive in diverse hostile environments, the structure of melanin remains unsolved. Nevertheless, ongoing research efforts have progressively revealed several notable structural characteristics of this enigmatic pigment, which will be the focus of this review. These compositional and organizational insights could further our ability to develop novel therapeutic approaches to combat fungal disease and enhance our understanding of how melanin is inserted into the cell wall.

Rna Sequencing Analysis Of The Msl2msl3, Crl, And Ggps1 Mutants Indicates That Diverse Sources Of Plastid Dysfunction Do Not Alter Leaf Morphology Through A Common Signaling Pathway, Darron R. Luesse, Margaret E. Wilson, Elizabeth S. Haswell

Biology Faculty Research

Determining whether individual genes function in the same or in different pathways is an important aspect of genetic analysis. As an alternative to the construction of higher-order mutants, we used contemporary expression profiling methods to perform pathway analysis on several Arabidopsis thaliana mutants, including the mscS-like (msl)2msl3 double mutant. MSL2 and MSL3 are implicated in plastid ion homeostasis, and msl2msl3 double mutants exhibit leaves with a lobed periphery, a rumpled surface, and disturbed mesophyll cell organization. Similar developmental phenotypes are also observed in other mutants with defects in a range of other chloroplast or mitochondrial functions, including …

Mechanisms Of Adaptation In The Newly Invasive Species Brachypodium Sylvaticum (Hudson) Beauv., Gina Lola Marchini

Dissertations and Theses

It is common knowledge that invasive species cause worldwide ecological and economic damage, and are nearly impossible to eradicate. However, upon introduction to a novel environment, alien species should be the underdogs: They are present in small numbers, possess low genetic diversity, and have not adapted to the climate and competitors present in the new habitat. So, how are alien species able to invade an environment occupied by native species that have already adapted to the local environment? To discover some answers to this apparent paradox I conducted four ecological genetic studies that utilized the invasive species Brachypodium sylvaticum (Hudson) …

Redescription And Lectotype Designation Of Vaejovis Lapidicola Stahnke, 1940 (Scorpiones: Vaejovidae), Matthew R. Graham

Euscorpius

The original description of the Arizona scorpion species Vaejovis lapidicola Stahnke, 1940 consists of a single paragraph. Furthermore, a holotype for this species was never designated. To remedy this, two syntypes of V. lapidicola were obtained for study. A male syntype is redescribed and designated the lectotype, and a female is assigned as a paralectotype. The taxonomic status of V. lapidicola is also confirmed.

Laterobasal Aculear Serrations (Las) In Scorpion Family Vaejovidae (Scorpiones: Chactoidea), Victor Fet, Michael E. Soleglad, Michael S. Brewer

Euscorpius

The discovery of a new structure on the laterobasal aspect of the telson aculeus is described here for the first time. This structure, termed the laterobasal aculear serrations (LAS), is a row of minute denticles located on each side of the aculeus base, found exclusively in scorpion family Vaejovidae, thus potentially providing a new synapomorphy for this New World family. The LAS structure of major representative vaejovid genera and Vaejovis groups is illustrated with SEM micrographs. Also provided is a comprehensive list of non-vaejovid Recent scorpion genera examined where the LAS structure was found to be absent.

The Effect Of Insularity On The Seasonal Population Structure Of Mesobuthus Gibbosus (Scorpiones: Buthidae), Dimitris Kaltsas, Iasmi Stathi, Moysis Mylonas

Euscorpius

We compared the population structure of Mesobuthus gibbosus from autumn to mid-summer at two similar phryganic ecosystems, one in continental Greece (Thessalia, near Volos city) and one in insular Greece (eastern Crete). Data were collected monthly using the capture-recapture method. At both sites, density was low during the cold period and increased towards summer. During the samplings, only a small percentage of each population was present. Population density and co-occurrence of scorpions with scorpions or other animals was higher in Crete than in Volos, probably due to the higher inter- and intraspecific competition in Volos. The population structure of the …

Duplication Of Pedipalp Segments In The Scorpion Androctonus Crassicauda (Olivier, 1807), Ayşegül Karataş, Mustafa Kürtüllü

Euscorpius

An unusual duplication of pedipalp segments is reported from the buthid scorpion Androctonus crassicauda. While the right pedipalp is normal, the anomaly described here occurred on the left pedipalp. This abnormally developed pedipalp included nine segments instead of normal six. It has two trochanters, two femurs and two patellae; the segments are arranged in the following order: coxa, trochanter I, femur I, patella I, trochanter II, femur II, patella II, chela hand, and movable finger. Morphometric measurements, photographs, and general features of the specimen are given.

Constellation Array In Scorpion Genera Paruroctonus, Smeringurus, Vejovoidus, And Paravaejovis (Scorpiones: Vaejovidae), Victor Fet, Michael E. Soleglad, Michael Brewer, David P. A. Neff, Michael L. Norton

Euscorpius

The constellation array (a recently discovered sensory structure on the fixed finger of pedipalp; Fet et al., 2006) is analyzed for a large set of species belonging to four genera in the family Vaejovidae: Paruroctonus, Smeringurus, Vejovoidus, and Paravaejovis. It is shown that this structure is an important taxonomic character. Two distinct configurations are identified, a two-sensilla array for Paruroctonus + Smeringurus + Vejovoidus and a three-sensilla array for genus Paravejovis, both differing from other vaejovid configurations so far investigated. The topology of these two array configurations are analyzed using landmark setae identified in this study.

Summer-Long Grazing Of High Vs. Low Endophyte (Neotyphodium Coenophialum)-Infected Tall Fescue By Growing Beef Steers Results In Distinct Temporal Blood Analyte Response Patterns, With Poor Correlation To Serum Prolactin Levels, Joshua J. Jackson, Merlin D. Lindemann, James A. Boling, James C. Matthews

Animal and Food Sciences Faculty Publications

Previously, we reported the effects of fescue toxicosis on developing Angus-cross steer growth, carcass, hepatic mRNA, and protein expression profiles of selected serum proteins, and blood clinical and chemical profiles, after summer-long grazing (85 days) of high endophyte (HE)- vs. low endophyte (LE)-infected fescue pastures. We now report the temporal development of acute, intermediate, and chronic responses of biochemical and clinical blood analytes determined at specified time intervals (period 1, day 0–36; period 2, day 37–58; and period 3, day 59–85). Throughout the trial, the alkaloid concentrations of the HE forage was consistently 19–25 times greater (P ≤ 0.002) …

Divergent Responses Of Cryptic Invasive Watermilfoil To Treatment With Auxinic Herbicides In A Large Michigan Lake, Syndell R. Parks

Masters Theses

Invasive plants are a major concern for environmental managers. Cryptic invasive taxa present additional challenges because of their potential to respond differently to management efforts. Invasive Eurasian watermilfoil (Myriophyllum spicatum) and hybrid watermilfoil (Myriophyllum spicatum x Myriophyllum sibiricum) cannot be reliably distinguished based on morphological characters and are therefore cryptic taxa. Laboratory studies show that on average, hybrid watermilfoil grows faster, branches more, and is less responsive to standard control measures developed for Eurasian watermilfoil. These laboratory results predict less effective control of hybrid watermilfoil in mixed populations treated uniformly with one of these control measures. However, to date there …

Adult Rainbow Trout Habitat Selection In The Henry’S Fork Of The Snake River, Idaho, Zachary Kuzniar

Masters Theses

Determining habitat selection for an organism across a geographic distribution is a primary challenge in modern behavioral ecology. Quantifying habitat use becomes increasingly difficult for species that exhibit complex life histories and complementary habitat requirements. Rainbow trout are an archetypal example due to their tendency to form local populations across a diversity of environments. The Henry’s Fork of the Snake River provides an opportunity to utilize microhabitat selection models to quantify stream features that may drive adult rainbow trout behavior at the local scale. In particular, within an 8.5 km, low gradient, groundwater-dominated reach that exhibits a general lack of …

Rnai-Mediated Control Of Aflatoxins In Peanut: Method To Analyze Mycotoxin Production And Transgene Expression In The Peanut/Aspergillus Pathosystem, Renee S. Arias, Phat M. Dang, Victor S. Sobolev

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The Food and Agriculture Organization of the United Nations estimates that 25% of the food crops in the world are contaminated with aflatoxins. That represents 100 million tons of food being destroyed or diverted to non-human consumption each year. Aflatoxins are powerful carcinogens normally accumulated by the fungi Aspergillus flavus and A. parasiticus in cereals, nuts, root crops and other agricultural products. Silencing of five aflatoxin-synthesis genes by RNA interference (RNAi) in peanut plants was used to control aflatoxin accumulation following inoculation with A. flavus. Previously, no method existed to analyze the effectiveness of RNAi in individual peanut transgenic events, …

The Status Of Fishes In The Missouri River, Nebraska: Selected Ancient Fishes, Kirk D. Steffensen

Transactions of the Nebraska Academy of Sciences and Affiliated Societies

Several ancient fish species have inhabited the Missouri River and its tributaries for thousands of years prior to major mainstem modifications and fragmentation. However post-anthropogenic modifications, populations of these ancient fish species have been highly diminished. Therefore, the objective of this study was to use historic and current ichthyological records to determine the past and present status for Chestnut Lamprey Ichthyomyzon castaneus, Silver Lamprey Ichthyomyzon unicuspis, Bowfin Amia calva, American Eel Anguilla rostrata, and Burbot Lota lota. Currently, these species are rarely captured and perhaps extirpated from Nebraska waters.

Characterization Of Cytidylyltransferase Enzyme Activity Through High Performance Liquid Chromatography, James Brault

Theses and Dissertations

The cytidylyltransferases are a family of enzymes that utilize cytidine 5â?? triphosphate (CTP) to synthesize molecules that are precursors to membrane phospholipids. There are four well known enzymes: CTP: phosphoethanolamine cytidylyltransferase (ECT), CTP: glycerol-3-phosphate cytidylyltransferase (GCT), 2-C-methyl-D-erythritol-4-phosphate cytidylyltransferase synthetase (CMS), and CTP: phosphocholine cytidylyltransferase (CCT). Previously, a radioisotope tagging method was employed to study cytidylyltransferase catalysis. Using CCT as a model, a method utilizing high performance liquid chromatography (HPLC) was developed to replace the radioisotope scintillation technique. The development of this new HPLC method is cheaper, more efficient, and more accurate than the previously established method.

The targets of separation …

Effects Of Oil Palm (Elais Guineensis) Fruit Extracts On Glucose Uptake Activity Of Muscle, Adipose And Liver Cells, Faez S, Muhajir H, Amin I, Zainah A

ASEAN Journal on Science and Technology for Development

The effect of oil palm (Elaeis guineensis) fruit aqueous extract (OPF) on glucose uptake activity of three different cell lines was investigated. The cell lines were incubated with different concentrations of OPF to evaluate the stimulatory effect of OPF towards glucose uptake activity of L6 myotubes, 3T3F442A adipocytes and Chang liver cell line. The glucose uptake activities of all tested cells were enhanced in the presence of OPF extract (basal condition). Nevertheless in combination of OPF extract and 100 nM insulin, the glucose uptake activity was only significantly enhanced in L6 myotubes and 3T3F442A adipocytes cell lines. The extracts enhanced …

Structural Comparison Of Tonb-Dependent Receptors In Bradyrhizobium Japonicum, Allie R. Casto

Senior Honors Projects, 2010-2019

Soybean is one of the leading crops grown in the United States. Nitrogen fertilizers are widely used in soybean production to increase crop yields. An alternative to nitrogen fertilizers is the symbiotic relationship between soybean and a species of nitrogen-fixing Gram-negative soil bacteria, Bradyrhizobium japonicum. The bacterium infects the roots of the soybean, forming nodules that perform nitrogen fixation. This study involves analysis of TonB-dependent receptors, intermembrane proteins responsible for iron uptake and, in some cases, symbiosis in B. japonicum. A comparison of sequence alignments and 3D structure predictions was used to identify potential symbiosis-specific structural domains within …