Life Sciences Commons^™

Fall Seed Guide 2015, Teshome Regassa, P. Stephen Baenziger, Stephen N. Wegulo, Greg Kruger, Dipak K. Santra

Department of Agronomy and Horticulture: Faculty Publications

Crops included in this guide are winter wheat, winter barley, and triticale. You may receive this guide in the mail or through the University of Nebraska Extension network. The data and other information this guide is can be found at our web site: http://cropwatch.unl.edu/varietytest/ and http://www.unl.edu/ncia. Additional information is available at the wheat variety virtual tour web site http://cropwatch.unl.edu/wheat/virtual or the winter wheat variety selection tool page http:// citnews.unl.edu/winter_wheat_tool/index.shtml. The last season was hard on Nebraska winter wheat. Emergence was poor in the west due to dry conditions. On the other hand, some plots in the east had to be …

The Use Of Assisted Reproductive Technologies To Improve Pregnancy Rates In White-Tailed Deer, Jaclyn C. Lambe-Steinmiller

LSU Doctoral Dissertations

Detection of estrus in white-tailed (WT) deer is laborious and often missed, as there are few visual cues presented during the 24 h period in which females are receptive to mating. Reproductive management of WT operations is improved by the use of fixed-timed artificial insemination (FTAI) protocols. Fixed-timed AI removes the need for estrus detection and ensures the breeding of an entire herd, as well as the ability to schedule fawning within predetermined timeframes. Two studies evaluated two FTAI regimens and their ability to synchronize estrous in WT. The first study implemented the administration of eCG 200 IU (im) at …

Advancing Understanding Of The Role Of Belowground Processes In Terrestrial Carbon Sinks Through Ground-Penetrating Radar: Final Report (De-Sc0008099), Frank P. Day

Biological Sciences Faculty Publications

Coarse roots play a significant role in belowground carbon cycling and will likely play an increasingly crucial role in belowground carbon sequestration as atmospheric CO₂ levels continue to rise, yet they are one of the most difficult ecosystem parameters to quantify. Despite promising results with ground-penetrating radar (GPR) as a nondestructive method of quantifying biomass of coarse roots, this application of GPR is in its infancy and neither the complete potential nor limitations of the technology have been fully evaluated. The primary goals and questions of this study fell into four groups: (1) GPR methods: Can GPR detect change …

Dynamic Ocean Management: Defining And Conceptualizing Real-Time Management Of The Ocean, Sara M. Maxwell, Elliot L. Hazen, Rebecca L. Lewison, Danial C. Dunn, Helen Bailey, Steven J. Bograd, Dana K. Briscoe, Sabrina Fossette, Alistair J. Hobday, Meredith Bennett

Biological Sciences Faculty Publications

Most spatial marine management techniques (e.g., marine protected areas) draw stationary boundaries around often mobile marine features, animals, or resource users. While these approaches can work for relatively stationary marine resources, to be most effective marine management must be as fluid in space and time as the resources and users we aim to manage. Instead, a shift towards dynamic ocean management is suggested, defined as management that rapidly changes in space and time in response to changes in the ocean and its users through the integration of near real-time biological, oceanographic, social and/or economic data. Dynamic management can refine the …

Transmission-Blocking Vaccines: Focus On Anti-Vector Vaccines Against Tick-Borne Diseases, Girish Neelakanta, Hameeda Sultana

Biological Sciences Faculty Publications

Tick-borne diseases are a potential threat that account for significant morbidity and mortality in human population worldwide. Vaccines are not available to treat several of the tick-borne diseases. With the emergence and resurgence of several tick-borne diseases, emphasis on the development of transmission-blocking vaccines remains increasing. In this review, we provide a snap shot on some of the potential candidates for the development of anti-vector vaccines (a form of transmission-blocking vaccines) against wide range of hard and soft ticks that include Ixodes, Haemaphysalis, Dermacentor, Amblyomma, Rhipicephalus and Ornithodoros species.

Identification And Comparative Analysis Of Subolesin/Akirin Ortholog From Ornithodoros Turicata Ticks, Hameeda Sultana, Unnati Patel, Daniel E. Sonenshine, Girish Neelakanta

Biological Sciences Faculty Publications

Background: Subolesin is an evolutionary conserved molecule in diverse arthropod species that play an important role in the regulation of genes involved in immune responses, blood digestion, reproduction and development. In this study, we have identified a subolesin ortholog from soft ticks Ornithodoros turicata, the vector of the relapsing fever spirochete in the United States.

Methods: Uninfected fed or unfed O. turicata ticks were used throughout this study. The subolesin mRNA was amplified by reverse transcription polymerase chain reaction (RT-PCR) and sequenced. Quantitative-real time PCR (QRT-PCR) was performed to evaluate subolesin mRNA levels at different O. turicata developmental stages …

Ecological Drivers And Habitat Associations Of Estuarine Bivalves, C. Seabird Mckeon, Björn G. Tunberg, Cora A. Johnston, Daniel J. Barshis

Biological Sciences Faculty Publications

Community composition of the infaunal bivalve fauna of the St. Lucie Estuary and southern Indian River Lagoon, eastern Florida was sampled quarterly for 10 years as part of a long-term benthic monitoring program. A total of 38,514 bivalves of 137 taxa were collected and identified. We utilized this data, along with sediment samples and environmental measurements gathered concurrently, to assess the community composition, distribution, and ecological drivers of the infaunal bivalves of this estuary system. Salinity had the strongest influence on bivalve assemblage across the 15 sites, superseding the influences of sediment type, water turbidity, temperature and other environmental parameters. …

A Popular And Potentially Sustainable Fishery Resource Under Pressure-Extinction Risk And Conservation Of Brazilian Sciaenidae (Teleostei: Perciformes), Ning L. Chao, Flávia L. Frédou, Manuel Haimovici, Monica B. Peres, Beth Polidoro, Marcelo Raseira, Rosana Subira, Kent E. Carpenter

Biological Sciences Faculty Publications

Croakers (Sciaenidae) are major fishery resource in Brazil; constituting 22% of marine and 9% of freshwater fishery landings. Croakers are subject to heavy fishing pressure throughout Brazil, but habitat alteration is also an important threat to regional populations. In this regional Sciaenidae assessment, each species was analyzed for relative risk of extinction, including the identification and quantification of the impact of major threats and existing conservation measures, based on application of the Categories and Criteria of the IUCN Red List of Threatened Species. Of the 52 species of Sciaenid fishes (34 marine and 18 freshwater) present in Brazilian waters, the …

Genetic Indicators Of Iron Limitation In Wild Populations Of Thalassiosira Oceanica From The Northeast Pacific Ocean, P. Dreux Chappell, Leann P. Whitney, Joselynn R. Wallace, Adam I. Darer, Samua Jean-Charles, Bethany D. Jenkins

OES Faculty Publications

Assessing the iron (Fe) nutritional status of natural diatom populations has proven challenging as physiological and molecular responses can differ in diatoms of the same genus. We evaluated expression of genes encoding flavodoxin (FLDA1) and an Fe-starvation induced protein (ISIP3) as indicators of Fe limitation in the marine diatom Thalassiosira oceanica. The specificity of the response to Fe limitation was tested in cultures grown under Fe-and macronutrient-deficient conditions, as well as throughout the diurnal light cycle. Both genes showed a robust and specific response to Fe limitation in laboratory cultures and were detected in small volume samples collected from …

Iron Deficiency Increases Growth And Nitrogen-Fixation Rates Of Phosphorus-Deficient Marine Cyanobacteria, Nathan S. Garcia, Feixue Fu, Peter N. Sedwick, David A. Hutchins

OES Faculty Publications

Marine dinitrogen (N²)-fixing cyanobacteria have large impacts on global biogeochemistry as they fix carbon dioxide (CO₂) and fertilize oligotrophic ocean waters with new nitrogen. Iron (Fe) and phosphorus (P) are the two most important limiting nutrients for marine biological N₂ fixation, and their availabilities vary between major ocean basins and regions. A long-standing question concerns the ability of two globally dominant N₂-fixing cyanobacteria, unicellular Crocosphaera and filamentous Trichodesmium, to maintain relatively high N₂-fixation rates in these regimes where both Fe and P are typically scarce. We show that under P-deficient …

Divergent Responses Of Atlantic Coastal And Oceanic Synechococcus To Iron Limitation, Katherine R. M. Mackey, Anton F. Post, Matthew R. Mcilvin, Gregory A. Cutter, Seth G. John, Mak A. Saito

OES Faculty Publications

Marine Synechococcus are some of the most diverse and ubiquitous phytoplankton, and iron (Fe) is an essential micronutrient that limits productivity in many parts of the ocean. To investigate how coastal and oceanic Atlantic Synechococcus strains acclimate to Fe availability, we compared the growth, photophysiology, and quantitative proteomics of two Synechococcus strains from different Fe regimes. Synechococcus strain WH8102, from a region in the southern Sargasso Sea that receives substantial dust deposition, showed impaired growth and photophysiology as Fe declined, yet used few acclimation responses. Coastal WH8020, from the dynamic, seasonally variable New England shelf, displayed a multitiered, hierarchical cascade …

The Effect Of Fluid Periodization On Athletic Performance Outcomes In American Football Players, Christopher W. Morris

Theses and Dissertations--Kinesiology and Health Promotion

For decades strength and conditioning professionals have been seeking optimal training volumes and intensities to yield maximum performance outcomes without the onset of injury. Unfortunately, current studies apply experimental training techniques without considering the individuals’ response to the imposed training load. Due to the vast genetic variability and extraneous environmental factors that affect one’s ability to recover, results from such studies are controversial and inconclusive. Athlete monitoring systems offer an objective assessment that is purported to evaluate an individual’s physiological readiness to adapt to an overload stimulus and thus allow for daily manipulations in training loads (i.e., fluid periodization). However, …

Effect Of Load Carriage On Tactical Performance, Justin M. Thomas

Theses and Dissertations--Kinesiology and Health Promotion

Special Weapons and Tactics (SWAT) operators are specially trained personnel that are required to carry equipment to perform high risk tasks. Given the need to carry this equipment, it is important to understand the potentially deleterious effect that the additional load may have on tactical performance. Furthermore, it is important to identify physical fitness characteristics that are associated with the potential decrement in performance. Therefore, the purpose of this study was to evaluate the effect of load carriage on tactical performance and identify fitness characteristics associated with any decrement in performance. Twelve male operators performed a simulated tactical test (STT) …

The Effects Of Spinal Manipulative Therapy On Isokinetic Strength And Postactivation Potentiation, Grant D. Sanders

Theses and Dissertations--Kinesiology and Health Promotion

Spinal manipulative therapy (SMT) is a therapeutic procedure employed by various healthcare practitioners for alleviating acute and chronic musculoskeletal complaints. This form of treatment is also delivered to enhance the performance and augment the rehabilitation of athletes. However, despite research findings alleging the strength-modulating effects of SMT alongside numerous professional athletes’ positive anecdotal claims concerning its results, the physiological processes to explain its effects remain largely unexplained. Therefore, the purpose of this work was to investigate the effects of SMT in a college-aged sample population with two experiments.

The first study examined the effect of SMT targeting the lumbosacral region …

An Acid-Bath Technique To Break Seed Dormancy In Common Sunflower, Helianthus L. Annuus (Asteraceae), Yuridia Patricia Gandy, Michael W. Persans, Kenneth R. Summy

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Chemistry)

The phenomenon of seed dormancy is widespread in plants and serves to prevent all or most of a given population from germinating at the “wrong” time, e.g., during an unusually mild fall in an area subject to typically harsh winters. Seed dormancy is an effective survival strategy in many plant populations, but may greatly complicate efforts to establish large cohorts of seedlings (groups of similar age or developmental stage) needed for re-search and other purposes. In an effort to break seed dormancy in common sunflower, Helianthus annuus L. (Asteraceae), we conducted experiments designed to compare germination times and overall germination …

A Program Designed To Address Academic Failure Due To Alcohol Abuse, Frank James Plateroti

Walden Dissertations and Doctoral Studies

This project study addressed the problem of alcohol and binge drinking at a local rural college campus in the Northeast United States and the lack of an effective long-term academic intervention program to address the problem. The purpose of this research study was to determine the prevalence of the problem of the alcohol abuse problem and to develop a long-term program that would respond to the problem of repeat alcohol offenders. Guided by Mezirow’s transformative learning theory, which holds that transformational learning causes changes in a learner that significantly shift the pattern of a learner’s future experiences, this study examined …

Relationships Between A Prenatal Nutrition Education Intervention And Maternal Nutrition In Ethiopia, Divya Lakshmi Selvakumar

Walden Dissertations and Doctoral Studies

In Ethiopia, 17% of pregnant women ages 18-49 are malnourished and have low awareness of prenatal nutrition, which may relate to increasingly high rates of maternal and infant mortality. The purpose of this mixed methods research study was to determine the effects of a community-based prenatal nutrition education intervention program on maternal nutrition knowledge and attitudes in the Alaje district of Ethiopia. The theoretical framework was Sen's capability theory of poverty, in which opportunities can lead to well-being and promote economic development. Research questions focused on the relationships among 8 independent variables-age, income source, degree of program implementation, marital status, …

Sewer Overflows And The Vector Mosquito Proximity To Human West Nile Virus Infections, Andrea Simone Bowers

Walden Dissertations and Doctoral Studies

DeKalb and Fulton Counties, which share the metropolitan Atlanta area, have seen an increase in West Nile infected vector mosquitoes; the increase is associated with close proximity to combined sewer overflow facilities. Despite completion of the remediation system in 2008, the mosquito population testing positive for West Nile virus has increased each year from 2010 through 2012. Guided by the Geographical Information System framework and using spatial analysis and regression analyses, this study described and quantified the relationship between sewer system overflows and amplification of vector mosquitoes; an additional goal was to investigate their proximity to human cases of West …

Correlation Analysis Of Climatic Variables, Migration And Dengue Cases In Southeast Florida, Brunilda Lugo

Walden Dissertations and Doctoral Studies

Dengue fever is a debilitating, viral, mosquito-borne disease occurring in tropical and subtropical areas in the world. The majority of dengue cases in the United States were acquired in endemic areas by travelers or immigrants. However, in recent years, autochthonous (locally acquired) dengue cases have been diagnosed in Florida. The purpose of this study was to find an association between potential risk factors and the expansion of dengue fever in the United States. Guided by the eco-bio-social framework, which offers a broad assessment of risk factors for the illness, a retrospective design was used with archival data to correlate changes …

Evaluation Of The System Attributes Of Timeliness And Completeness Of The West Virginia Electronic Disease Surveillance System' Nationaledss Based System, Rebecca Lee Fahey

Walden Dissertations and Doctoral Studies

Despite technological advances in public health informatics, the evaluation of infectious

disease surveillance systems data remains incomplete. In this study, a thorough

evaluation was performed of the West Virginia Electronic Disease Surveillance System

(WVEDSS, 2007-2010) and the West Virginia Electronic Disease Surveillance System NationalEDSS -Based System (WVEDSS-NBS; March 2012 - March 2014) for Category II infectious diseases in West Virginia. The purpose was to identify key areas in the surveillance system process from disease diagnosis to disease prevention that need improvement. Grounded in the diffusion of innovation theory, a quasi-experimental, interrupted, time-series design was used to evaluate the 2 data …

Examining Behavioral Reactivity And Cognitive Differences Within The Chc Theory Of Intelligence Among Children., Steven Matthew Jozwiak

Walden Dissertations and Doctoral Studies

Linking cognition and behavior has long been an area of interest to the field of psychology in its endeavors to understand what innate factors influence human behavior. To date, the majority of research linking emotional reactivity to cognition has focused on single areas of intellectual functioning on specific diagnostic profiles or learning disorders rather than a comprehensive comparison to cognitive profile typology. Nearly all the research conducted to date continues to define cognition and emotion as disparate entities, rather than exploring a more integrated view of emotion and cognition. The purpose of this quantitative study was to examine cognitive profile …

Uric Acid Level Is Associated With Postprandial Lipemic Response To A High Saturated Fat Meal, Roy Gail Cutler

Walden Dissertations and Doctoral Studies

Hyperlipidemia caused by a diet high in saturated fat can lead to visceral fat weight gain, obesity, and metabolic syndrome. Being over-weight from visceral fat has been linked to increased risk of developing most age-related diseases and disability, along with a lower income potential and quality of life. However, researchers are just beginning to understand the biological mechanisms that regulate the conversion of excess calories into visceral fat storage rather than glycogen or muscle. Epidemiological studies have repeatedly shown a comorbid association between age-related diseases involving hyperlipemia and circulating levels of uric acid, but not a direct association. This study …

Relationships Between The Coordinated School Health Program And Childhood Obesity In Tennessee, Tekeela Shonta Green

Walden Dissertations and Doctoral Studies

Childhood obesity rates have increased nationwide, but rates of childhood obesity are higher in Tennessee (TN). The Coordinated School Health (CSH) Program is a multicomponent approach designed to improve academics and health in children in Tennessee; however, researchers have not evaluated the 8 components of the plan and the impact it has on weight in children. The purpose of the study was to determine if a relationship existed between the Coordinated School Health Program and obesity in children. The conceptual framework for this study was Bandura's social cognitive theory. One hundred forty CSH coordinators in TN were surveyed and the …

The European Union’S Potential Contribution To Protect Marine Biodiversity In The Changing Arctic: A Roadmap, Nengye Liu, Elizabeth Kirk

Research Collection Yong Pung How School Of Law

This article provides a roadmap for possible European Union (eu) action in the protection of marine biodiversity in the changing Arctic. First the eu’s competence in the Arctic is briefly introduced. Then the discussion turns to the eu’s potential external and internal actions in shipping, fisheries and offshore oil and gas operations that could possibly enhance the international legal regime for the protection of marine biodiversity in the Arctic. The eu’s role vis-à-vis the global biodiversity regime and cross-cutting measures under the United Nations Convention on the Law of the Sea are discussed together in the final part.

Constitutive Activity In Orphan G Protein Coupled Receptors, Adam Lee Martin

Doctoral Dissertations

"The goal of this research was to use a distal signaling pathway analysis to evaluate the extent of agonist independent constitutive signaling among orphan class-A G protein coupled receptors (GPCRs). These receptors translate extracellular signals via conformational change into intracellular activation of different G proteins and subsequent second messenger synthesis. These small molecules regulate cellular biochemistry, eventually leading to nuclear signaling that results in changes in gene expression. Some GPCRs are capable of signaling in the absence of an activating ligand, a phenomenon called constitutive activity that is inhibited via an "inverse-agonist". The use of cAMP dependent Luciferase expression is …

Evolutionary Dynamics Of Speciation And Extinction, Dawn Michelle King

Doctoral Dissertations

Presented here is an interdisciplinary study that draws connections between the fields of physics, mathematics, and evolutionary biology. Importantly, as we move through the Anthropocene Epoch, where human-driven climate change threatens biodiversity, understanding how an evolving population responds to extinction stress could be key to saving endangered ecosystems. With a neutral, agent-based model that incorporates the main principles of Darwinian evolution, such as heritability, variability, and competition, the dynamics of speciation and extinction is investigated. The simulated organisms evolve according to the reaction-diffusion rules of the 2D directed percolation universality class. Offspring are generated according to one of three reproduction …

Harvest Demographics Of Temperate-Breeding Canada Geese In South Dakota, 1967–1995, Jeffrey S. Gleason, Jonathan A. Jenks, David E. Naugle, Paul W. Mammenga, Spencer J. Vaa, Jennifer M. Pritchett

Human–Wildlife Interactions

In South Dakota, breeding giant Canada geese (Branta canadensis maxima) have increased substantially, and harvest management strategies have been implemented to maximize hunting opportunity (e.g., special early-September seasons) on local, as well as molt-migrant giant Canada geese (B. c. interior) while still protecting lesser abundant Arctic breeding Canada geese and cackling geese (e.g., B. hutchinsii, B. minima). Information on important parameters, such as survival and recovery rates, are generally lacking for giant Canada geese in the northern Great Plains. Patterns in Canada goose band recoveries can provide insight into the distribution, chronology, and harvest pressures …

Methodology For Estimating Deer Browsing Impact, Timothy G. Pierson, David S. Decalesta

Human–Wildlife Interactions

Because there were no reliable indicators of deer browsing on tree seedling regeneration, we developed methodology that can be used to measure deer browsing impact. We compared 11 years (2002 to 2012) of annual estimates of deer density with coarse (percent-plots-no-regeneration, percent-plots-no-impact) and fine (3 levels of impact on 6 indicator seedling species) indicators within a 29,642-ha study area in northwestern Pennsylvania. Coarse and fine measures met established criteria for indicators of environmental stress (e.g., high deer density); they were predictive of stresses that can be: avoided by management; integrative with causes of stress; responsive to disturbances and changes over …

Changes In Distribution Of Canada Geese Nesting In Arkansas, M. Eliese Ronke, David G. Krementz

Human–Wildlife Interactions

The reintroduced Canada goose (Branta canadensis) population in Arkansas has grown in range and abundance in recent decades. We determined the geographic range of Arkansas resident Canada geese from 2004 to 2012 using volume contour maps from citizen science observations using eBird, a citizen science website, and hunter recovery locations from the U.S. Geological Survey Bird Banding Laboratory. Resulting maps indicate an increase in Canada goose encounters toward northwestern and southwestern Arkansas from the original relocations in the Arkansas River valley. We examined movement of Canada geese banded and recovered in Arkansas by determining the distance and angle …

Characterizing Nontarget Species Use At Bait Sites For White-Tailed Deer, Brent Bowman, Jerrold L. Belant, Dean E. Beyer Jr., Deborah Martel

Human–Wildlife Interactions

Baiting white-tailed deer (Odocoileus virginianus) has evolved into a controversial issue of wildlife management. During August–September 2012, we established a grid of 64 cameras in the Upper Peninsula of Michigan at sites baited with corn that simulated legal bait sites for white-tailed deer to characterize presence, diversity, and frequency of species use. We detected >20 species of wildlife that visited bait sites. We categorized 3,177 of 11,194 images as independent detections (i.e., species detected >1 hour apart). White-tailed deer had the greatest detection rate (47%), but overall detections of nontarget species was slightly greater (53%). Most frequent nontarget …