Life Sciences Commons^™

Effect Of A Simulate Show Jumping Competition On The Blood Gas Profile Of Horses Trained For Show Jumping, Francesco Fazio, Vanessa Messina, Stefania Casella, Claudia Giannetto, Simona Marafioti, Giuseppe Piccione

Turkish Journal of Veterinary & Animal Sciences

The aim of the present study was to evaluate the changes in the blood gas profile of jumpers after warm up and after a show jumping competition. For this purpose, 10 Sella Italiana geldings, 7 years old and clinically healthy, were used. The horses were trained to take part in show jumping competitions. Blood samples were collected at rest, after the warm up, immediately after the exercise, and 45 min after the end of exercise. Standard bicarbonate concentration (SBC), hydrogen carbonate ion (HCO_3^-), total carbon dioxide (TCO_2), oxygen capacity (O_2Cap), oxygen content (O_2Ct), base excess of the blood (BE-b), base …

The Effect Of Oxytocin And Cloprostenol Application Via Umbilical Artery Immediately After Dystocia On Time And Rate Of Fetal Membrane Removal In Cows, Yaşar Akar, Ömer Kizil, Nevzat Saat, Murat Yüksel

Turkish Journal of Veterinary & Animal Sciences

The aim of this clinical study was to investigate the effects of oxytocin and cloprostenol application via umbilical artery on the time and rate of fetal membrane removal in cows with dystocia immediately after parturition. Metabolic profiles indicated certain serum biochemical parameters that were also determined in the cows. The study was performed on 60 cows with dystocia. All of the cows were randomly divided into 3 equal subgroups. The first group was injected with 100 IU oxytocin, while the second group was treated with 0.15 mg cloprostenol and the third group (the control group) received 10 mL 0.9% NaCl …

The Influence Of Egg Shell Crack Types On Hatchability And Chick Quality, Mozhdeh Moosanezhad Khabisi, Ahmad Salahi, Seyed Naser Mousavi

Turkish Journal of Veterinary & Animal Sciences

Fertile eggs were obtained from a commercial flock of Ross broiler breeders (51 weeks old) and candled to determine the presence of hairline or star cracks. Eggs (5400 total) were assigned in equal numbers to 3 treatments (hairline-cracked, star-cracked, and normal eggs) and incubated for 21 days. Upon hatching, 10 chicks per treatment were euthanized, and organ weights were measured. Compared with normal eggs, the presence of egg shell cracks (regardless of type) resulted in higher egg weight loss at the time of transfer; a decrease in hatchability, hatch of fertile eggs, chick length, yolk-free body mass (P < 0.01), and percentage of hatchling breast and liver weight (P < 0.05); and an increase in the contamination rate of eggs (P < 0.01). Weight loss in hairline-cracked eggs was significantly higher than in star-cracked eggs (P < 0.001). Percentage hatchability and body weight of chicks hatched from star-cracked eggs was higher than in chicks from hairline-cracked eggs (P < 0.001). Crack type did not have a significant effect on chick length and body weight uniformity; however, the Pasgar score as a quality indicator was higher in chicks hatched from star-cracked eggs than those from hairline-cracked eggs (P < 0.05). There were no significant differences among treatments in terms of heart and small intestine weight. The contamination rate of hairline-cracked eggs was higher than that of star-cracked (P < 0.01). A higher incidence of embryonic mortality was observed during days 1-8 of incubation for shell-cracked eggs (P < 0.001). Total embryonic mortality in hairline-cracked, star-cracked, and normal eggs was 51.1%, 36.6%, and 13.3%, respectively. This study showed that egg shell cracks reduced incubation parameters and chick quality. The negative effects of hairline cracks were more pronounced than those of star cracks.

Immunopotency Of Novel Oil Adjuvant Vaccines Employing Pasteurella Multocida Biofilm And Capsule Enhanced Organisms In Ducklings, Ramachandranpillai Rajagopal, Govindapillai Krishnan Nair, Mini Mangattumuruppel, Joseph Leo, Saseendranath Raghavan Mapranath

Turkish Journal of Veterinary & Animal Sciences

Pasteurella multocida-a gram-negative bacterium with a broad host range and ubiquitous distribution—is a serious problem in ducks, and the best method of control is vaccination with locally prevalent strains. The present study was conducted to compare the immunopotency of vaccines employing P. multocida biofilms and capsule enhanced organisms with ordinary broth grown organisms in 1-month-old ducklings, by measuring the humoral immune response and the protection conferred by each vaccine during homologous challenge with virulent organisms. Formalin inactivated oil adjuvant bacterin vaccines were prepared from Pasteurella multocida A: 1. They were grown in conventional broth, capsule enhancement medium, and under biofilm …

An Immunohistochemical Study On The Distribution Of Endocrine Cells In The Digestive Tract Of Gray Goose (Anser Anser), Jun Yang, Lei Zhang, Xin Li, Leii Zhang, Xiangjiang Liu, Kemei Peng

Turkish Journal of Veterinary & Animal Sciences

The objective of this study was to investigate the morphology and the distribution of 5-hydroxytryptamine (5-HT), somatostatin (SS), gastrin (Gas), glucagon (Glu), and substance P immunoreactive (IR) cells in the digestive tract of gray goose by the immunohistochemical streptavidin-peroxidase method. The samples were taken from 10 healthy adult gray geese. The results showed that 5 kinds of IR cells were mainly distributed between the mucous epithelium and intestinal gland. The number of 5-HT-IR cells was highest in the rectum and duodenum, but none were observed in the pylorus. SS-IR cells appeared in great numbers in the pylorus, duodenum, and cecum; …

Effects Of Inulin And \Beta-Glucan Supplementation In Broiler Diets On Growth Performance, Serum Cholesterol, Intestinal Length, And Immune System, Asia Sanhouri Elrayeh, Gülteki̇n Yildiz

Turkish Journal of Veterinary & Animal Sciences

This study was designed to investigate the effects of a supplement of inulin and \beta-glucan into broiler rations on growth performance, serum cholesterol, intestinal length, and immune system. A total of 320 male 1-day-old Ross PM3 broiler chicks were divided into 1 control and 3 treatment groups, each comprising 80 birds. Each group was divided into 4 replicate groups of 20 birds each. The treatment groups were supplemented with 0.7% inulin, 0.014% \beta-glucan, and 0.7% inulin + 0.014% \beta-glucan, respectively. The experiment lasted for 6 weeks. Immunomodulatory roles were also investigated by measuring the effects on specific antibody titer, and, …

Protective Effect Of N-Acetyl-L-Cysteine Against Acrylamide-Induced Oxidative Stress In Rats, Ebru Işik Alturfan, Ayfer Beceren, Ahmet Özer Şehi̇rli̇, Zeynep Emel Demi̇ralp, Göksel Şener, Gülden Zehra Omurtag

Turkish Journal of Veterinary & Animal Sciences

Acrylamide (AA), used in many fields, from industrial manufacturing to laboratory work, is also formed during the heating process through the interactions of amino acids. Therefore, AA poses a significant risk for both human and animal health. This study aimed to elucidate whether N-acetyl-L-cysteine (NAC) treatment could modulate AA-induced oxidative changes in the brain, lung, liver, kidney, and testes tissues of the rat. Rats were divided into 4 groups, as the control (C), NAC [150 mg/kg intraperitoneally (i.p.)], AA (40 mg/kg i.p.), and NAC + AA groups. After 10 days, the rats were decapitated and the tissues were excised. Malondialdehyde …

Neuronal And Extraneuronal Lipofuscinosis In Merino Sheep Grazing Asphodelus Aestivus Seeds In Western Turkey, Sümbül Serap Bi̇ri̇nci̇oğlu, Wolfgang Schmahl, Hamdi̇ Avci

Turkish Journal of Veterinary & Animal Sciences

Neuronal and extraneuronal lipofuscinosis was determined in Merino sheep (6 sheep and 3 heads of sheep) of 2-3 years old that had grazed on Asphodelus aestivus seeds. Clinically, the sheep exhibited tremors, paresis, oral and nasal discharge, and severe respiratory distress. Haemorrhagic necrotic tracheitis, aspiration pneumonia, and subacute enteritis were observed in 6 sheep during macroscopic examinations. The brains of the animals were of firm consistency and yellowish brown in colour. Histological examination of the central nervous system revealed that most of the cytoplasm of neurons contained yellowish brown pigment granules. Pigmentations were particularly detected in the pons, medulla oblongata, …

Changes In Cell Wall Compositions And Degradation Kinetics Of Electron Beam-Irradiated Sugarcane Bagasse, Parvin Shawrang, Abbas Majdabadi, Ali Asghar Sadeghi

Turkish Journal of Veterinary & Animal Sciences

Electron beam irradiation of sugarcane bagasse at doses of 250 and 500 kGy could change its cell wall composition and ruminal fiber degradation characteristics by breaking off the lignocellulosic bonds. Nylon bags of untreated or electron beam-irradiated bagasse were suspended in the rumen of 3 fistulated rams for up to 72 h, and the resulting data were fitted to a nonlinear degradation model to calculate the degradation parameters of dry matter (DM) and neutral detergent fiber (NDF). Irradiation had no effect on crude protein, ether extract, and ash, but resulted in a linear decrease of the cell wall contents. An …

Prevalence And Factors Affecting The Presence Of Campylobacter Spp. In Broiler Carcasses In Bulgaria, Hristo Daskalov, Alexander Maramski

Turkish Journal of Veterinary & Animal Sciences

In an 11-month study, 292 samples from 13 slaughterhouses in Bulgaria were investigated for the presence of Campylobacter spp., and some factors affecting Campylobacter prevalence were analyzed. The study revealed that 44.9% of the samples were positive for Campylobacter spp. The main species detected were C. coli and C. jejuni. There was also one confirmed C. lari isolate. The C. coli strains prevailed over C. jejuni at 61.8% and 37.4%, respectively. A seasonal variation in the Campylobacter spp. presence in slaughter broiler carcasses was found with a predominance of positive isolates during June and July 2008. The conventional type of …

Fecal Progesterone Analysis For Monitoring Reproductive Status In Dairy Goats, Amelia Miranda Morgiana Jack, Chao-Chin Chang, Huo-Cheng Peh, Jacky Peng-Wen Chan

Turkish Journal of Veterinary & Animal Sciences

The present study was undertaken to evaluate the use of fecal progesterone (FP_4) concentration for early pregnancy diagnosis, estrus detection, and predicting the litter size and parturition date in dairy goats. Fecal samples were collected from 17 hand-mated goats 3 times a week for 22-23 weeks, beginning on the day of mating. The levels of FP_4 were determined by enzyme-linked immunosorbent assay (ELISA). Significant positive correlations were found between the levels of serum P_4 and FP_4 (r = 0.8787, n = 13). Pregnancy diagnosis was performed at 2 months postmating with transabdominal ultrasonography and confirmed upon birth of the offspring. …

Determination Of Egg Consumption And Consumer Habits In Turkey, Cengi̇zhan Mizrak, İsmai̇l Durmuş, Serdar Kamanli, Şahnur Erdoğan Demi̇rtaş, Süleyman Kalebaşi, Ender Karademi̇r, Murat Doğu

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to determine egg consumption habits in Turkey. Questionnaires prepared to determine habits regarding egg consumption were supplied to 2241 families. Sampling sizes and sample addresses were provided by the Turkish Statistical Institute. Data obtained through the questionnaires were analyzed using SPSS. Of the families that participated in the study, 98% consume eggs and the number of eggs consumed per person was 158 per year. In addition, 67.82% of the eggs consumed were purchased from supermarkets and 62.40% of the consumers pay most of their attention to the production date. The eggs were most often …

A Note On Phthirapteran Ectoparasites Infesting Yellow-Legged Green Pigeon Treron Phoenicoptera (Columbiformes: Columbidae), Aftab Ahmad, Gaurav Arya, Nayanci Bansal, Oldrich Sychra, Arun Kumar Saxena

Turkish Journal of Veterinary & Animal Sciences

The present report records the simultaneous occurrence of ischnoceran lice Columbicola phoenicopterae and Coloceras setosum on the Yellow-legged Green Pigeon in India. The prevalence, intensity of infestation, range of infestation, and nature of the antennal sensilla of these 2 species on 12 of these pigeons have also been recorded.

Protective Effects Of Salvia Officinalis Extract Against Cyclophosphamide-Induced Genotoxicity And Oxidative Stress In Rats, Fulya Üstün Alkan, Feraye Esen Gürsel, Ati̇la Ateş, Mustafa Özyürek, Kubi̇lay Güçlü, Mehmet Altun

Turkish Journal of Veterinary & Animal Sciences

Medicinal plants, especially rich in polyphenolic compounds, have been suggested to be chemopreventive on account of antioxidative properties. Salvia officinalis L., an aromatic and medicinal plant, is widely used in folk medicine and is well known for its antioxidant properties. Therefore, the present study was designed to investigate the antioxidative, possible genotoxic, and antigenotoxic potency of S. officinalis extract against cyclophosphamide (CYP)-induced oxidative stress and genotoxicity in Wistar albino rats. Animals were orally dosed with S. officinalis extract (50, 100, and 150 mg/kg body weight) for 7 days before the administration of a single intraperitoneal dose of CYP (40 mg/kg …

Ultrasonographic Evaluation Of Cricoarytenoideus Lateralis Muscle In Standardbred Horse, Fulvio Laus, Emanuele Paggi, Matteo Cerquetella, Beniamino Tesei

Turkish Journal of Veterinary & Animal Sciences

The cricoarytenoideus lateralis muscle (CALM) is an adductor of the arytenoid cartilages but is not directly involved in the pathogenesis of recurrent laryngeal neuropathy (RLN) as the abductor cricoarytenoideus dorsalis muscle (CADM) is. Nevertheless, the CALM is the first muscle to experience a neurogenic atrophy in the course of RLN and this feature explains its potential importance in RLN diagnosis. On 30 gelding and 30 female Standardbred horses weighing about 500 kg and aged between 3 and 9 years, an ultrasound examination of the larynx was performed in order to assess shape, size, and echogenicity of the CALMs. The mean …

Establishment And Biological Research Of The Jining Grey Goat Fibroblast Line, Chunyu Bai, Dianjun Wang, Xiaohua Su, Minghai Zhang, Weijun Guan, Yuehui Ma

Turkish Journal of Veterinary & Animal Sciences

Using Jining Grey goat ear marginal tissues as experimental materials, we succeeded in establishing a fibroblast cell bank, including 32 samples, by means of a primary explant and cryopreservation technique. The results from the biological analysis suggested that the population doubling time of the cell line was approximately 48 h. The diploid accounted for 98.35%, the isozyme analysis of the lactic dehydrogenase and malic dehydrogenase disproved cross contamination, and the results of the bacterium, fungi, virus, and mycoplasma detection tests were all negative. The transfection rates of 3 fluorescent protein genes were high, which supported the opinion that exogenous genes …

Prevalence And Patterns Of Antimicrobial Resistant Of Fecal Escherichia Coli Isolates Among Pigs On Eight Farrow-To-Finish Farms And Their Production Environments In Shandong Province, China, Hongchao Zhou, Lin Liu, Zhaofang Tian, Wenjuan Zhang

Turkish Journal of Veterinary & Animal Sciences

The main objectives of this study were to determine the prevalence and antimicrobial resistance patterns of Escherichia coli isolates in feces of pigs and their production environments on farms. A total of 560 isolates of Escherichia coli, from 112 samples obtained from weaner and finisher pigs, feed, water, and house sparrows on 8 farrow-to-finish farms, were tested for susceptibility to 16 antimicrobials. The prevalence of resistance varied widely (0.0% to 69.5%) among the antimicrobials tested. From the tested swines, 75.75% of the isolates were resistant to one or more antimicrobials. Resistance was significantly more frequent (P = 0.005, P < 0.01) in swine isolates compared to those from feed, water, and sparrows and significantly more frequent (P = 0.007, P < 0.01) in weaner pigs compared to finisher pigs. The findings indicate that resistance to a broad range of antimicrobials was prevalent among fecal E. coli isolates of swine on the study farms. Fortunately, E. coli was not isolated from the feed, water, and house sparrows.

Comparison Of Different Lactation Curve Models In Sahiwal Cows, Vilas Dongre, Ravindar Singh Gandhi, Avtar Singh

Turkish Journal of Veterinary & Animal Sciences

The present investigation was carried out on 12,859 fortnightly test-day milk yield records of first lactation pertaining to 643 Sahiwal cows spread over a period of 49 years (1961-2009), maintained at the National Dairy Research Institute, Karnal, India. The comparison of 3 lactation curve models, i.e. the quadratic model, gamma-type function, and mixed log function, was done using fortnightly test-day milk yield records. The mixed log function was the best fit, with the highest (88.75%) coefficient of determination (R2 value) and the lowest (0.08 kg) root mean square error (RMSE) value, whereas the lowest (56.93%) R2 value was observed in …

Prevention Of Predicted Neonatal Isoerythrolysis With Jaundice Foal Agglutination Test In A Newborn Foal, Aykut Göktürk Üner, Güney Kaya, Aydin Oltu

Turkish Journal of Veterinary & Animal Sciences

This case report aims to highlight the utility of the jaundice foal agglutination (JFA) test used in the prevention of neonatal foal death caused by equine neonatal isoerythrolysis. The colostrum and the serum of a Friesian mare with repeated neonatal foal deaths and the whole blood of her newborn foal were tested for 5 days with the JFA test to detect a probable incompatibility. For the first 2 days, the JFA test gave a significantly positive reaction, indicating the existence of antibodies in the colostrum of the mare against the foal's erythrocyte antigens. The foal was not allowed access to …

A Neurological Foundation For Freedom, Nita A. Farahany

Faculty Scholarship

No abstract provided.

A Qcm-Adhering Bacillus Anthracis Soil Phage Assemblage Is Less Diverse Than A Total B. Anthracis Soil Phage Assemblage, Gabriel David Connell

Dissertations and Theses @ UNI

Recently, phages have been used as affinity reagents in quartz crystal microbalance (QCM)-based biodetection methods. The goal of this study was to isolate and characterize a Bacillus cereus group soil-phage assemblage, which was isolated from 5 grams of local topsoil, and compare its population structure to that of a similar phage assemblage that had been additionally selected by adhering to a QCM electrode. Viruses aren't well-defined in terms of 'species', therefore operational taxonomic units (OTUs) were defined by structural protein profiles from sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). We were able to obtain a conservative estimate of soilphage 'species' richness …

Serine 910 Phosphorylation Of Focal Adhesion Kinase Is Critical For Costamere Assembly, Miensheng Chu

Dissertations

Tyrosine-phosphorylated FAK is required for the hypertrophic response of cardiomyocytes to growth factors and mechanical load, but the role of FAK serine phosphorylation in this process is unknown. Endothelin-1 (ET-1; 1-100nM, 2-30min) and other hypertrophic factors induced a time- and dose-dependent increase in FAK-S910 phosphorylation in neonatal rat ventricular myocytes (NRVM). FAK-S910 phosphorylation required ET_AR-dependent activation of PKCδ and Src via parallel Raf-1→MEK1/2→ERK1/2 and MEK5→ERK5 signaling pathways. Using co-immunoprecipitation, TIRF-microscopy and FRAP, ET-1 stimulation of NRVM expressing a nonphosphorylatable, S910A-FAK mutant decreased the interaction of paxillin and vinculin within costameres. This interaction was important in stabilizing α-actinin within …

Molecular Mechanisms Regulating Chemokine Receptor Cxcr4 Signaling And Trafficking, Rohit Malik

Dissertations

CXCR4 is a G protein-coupled receptor (GPCR) that binds to the chemokine, stromal cell-derived factor-1 (SDF-1alpha; a.k.a. CXCL12). The SDF-1alpha/CXCR4 signaling axis plays an essential role during embryogenesis in the development of the heart, brain and vasculature and in the adult mediating immune cell trafficking and stem cell homing to the bone marrow. Dysregulation of SDF-1alpha/CXCR4 signaling is linked to several pathological conditions, including cardiovascular disease, immunological disorders as well as cancer growth and metastasis. However, the mechanisms that govern CXCR4 signaling remain poorly understood. In this dissertation project, we attempt to further our understanding of the molecular mechanisms that …

An Investigation Of The Phospholamban-Serca Regulatory Interaction With Fluorescence Resonance Energy Transfer, Philip Adam Bidwell

Dissertations

With Fluorescence Resonance Energy Transfer (FRET), we are able to detect changes in the structure and affinity of the PLB-SERCA regulatory complex in live cells. Using this approach, we have detected a high level of PLB-SERCA interaction even at Ca2+ concentrations known to fully relieve PLB inhibition of SERCA, suggesting that dissociation is not required for relief of inhibition. We also detect no real-time change in PLB-SERCA binding over the course of a single Ca2+ transient in paced myocytes. The effect of Ca2+ on the PLB-SERCA interaction is best described as a reduced affinity with no change in the structure …

Receptor-Mediated Hypertrophic Signaling Via Protein Kinase D And Histone Deacetylase 5 In Adult Myocytes, Chia-Wei Jenny Chang

Dissertations

Hemodynamic stress and neurohumoral signaling are common causes of cardiac hypertrophy. These extrinsic stress stimuli typically act on GPCR and induce a cascade of signal transduction to re-program terminally differentiated myocytes to grow in length or width. The compensatory hypertrophic response can enhance cardiac output briefly due to increased work load. However, prolonged stress results in maladaptive changes in the heart and gradually deteriorates ventricular function to supply blood throughout the body. Sustained hypertrophic signaling can also progress toward heart failure.

My dissertation research focuses on the hypertrophic signaling in adult cardiac myocytes in response to neurohumoral stimuli, ET-1 and …

Effects Of Binge Alcohol Exposure On Canonical Wnt Signaling During Fracture Repair, Kristen Leigh Lauing

Dissertations

Excessive alcohol consumption is associated with increased fracture risk, delayed bone healing and fracture non-union. Binge alcohol consumption has occurred in 25-40% of all orthopaedic trauma cases. The canonical Wnt pathway, through tight regulation of stabilized beta-catenin, plays an essential and pivotal role in the formation of new bone and cartilage to initiate bone repair. We sought to determine the molecular mechanisms behind alcohol-related fracture complications by testing the hypothesis that binge alcohol exposure deregulates canonical Wnt signaling in the fracture callus, leading to impaired healing.

To test this, C57BL/6 or beta-catenin/TCF-reporter male mice were exposed to intraperitoneal alcohol or …

The Role Of Cyp33 In Mll Mediated Gene Repression, Steven D. Poppen

Dissertations

Mixed Lineage Leukemia (MLL) is a multidomain protein whose gene is translocated in a subset of AML leukemias. Translocation of the MLL gene is present in approximately five percent of adult acute leukemias and ten percent of pediatric leukemias (Daser, A 2004, Look, A 1997, Huret, J 2001) Patients presenting in the clinic at the time of diagnosis with an MLL fusion have been shown to respond poorly to treatment and have a worse prognosis than matched wild type MLL patients (Rubnitz, J 1994, Rubnitz, J 1999). Novel therapies therefore are needed in order to more effectively treat patients with …

Compositional Determinants Of The Pharmacological Actions Of Heparins, Angel Lee Gray-Shah

Dissertations

This dissertation primarily focuses on how differences in molecular weight (MW) and structural composition affect the pharmacological activity of heparin and its derivatives. Heparins are a mixture of glycosaminoglycans chains which are used to prevent thrombosis in a number of clinical indications. Heparins promote the inhibition of blood coagulation via their plasmatic cofactors antithrombin (AT) and heparin cofactor II (HCII).

In these studies, various heparins with molecular weights ranging from 2.6 to 16.5 kDa were investigated. Not only the molecular weight but also the oligosaccharide composition greatly varied in these agents. One of the major objectives of this research was …

Inefficient Replication Reduces Reca-Mediated Repair Of Uvdamaged Plasmids Introduced Into Competent Escherichia Coli, Harout Arthur Jeiranian, Charmain T. Courcelle, Justin Courcelle

Biology Faculty Publications and Presentations

Transformation of Escherichia coli with purified plasmids containing DNA damage is frequently used as a tool to characterize repair pathways that operate on chromosomes. In this study, we used an assay that allowed us to quantify plasmid survival and to compare how efficiently various repair pathways operate on plasmid DNA introduced into cells relative to their efficiency on chromosomal DNA. We observed distinct differences between the mechanisms operating on the transforming plasmid DNA and the chromosome. An average of one UV-induced lesion was sufficient to inactivate ColE1-based plasmids introduced into nucleotide excision repair mutants, suggesting an essential role for repair …

Uvrd Participation In Nucleotide Excision Repair Is Required For The Recovery Of Dna Synthesis Following Uv-Induced Damage In Escherichia Coli, Kelley Nicole Newton, Charmain T. Courcelle, Justin Courcelle

Biology Faculty Publications and Presentations

UvrD is a DNA helicase that participates in nucleotide excision repair and several replication-associated processes, including methyl-directed mismatch repair and recombination. UvrD is capable of displacing oligonucleotides from synthetic forked DNA structures in vitro and is essential for viability in the absence of Rep, a helicase associated with processing replication forks. These observations have led others to propose that UvrD may promote fork regression and facilitate resetting of the replication fork following arrest. However, the molecular activity of UvrD at replication forks in vivo has not been directly examined. In this study, we characterized the role UvrD has in processing …