Life Sciences Commons^™

Regulation Of The Heat Shock Response By Thiol-Reactive Compounds In The Yeast Saccharomyces Cerevisiae, Yanyu Wang

Dissertations and Theses (Open Access)

Cells govern their activities and modulate their interactions with the environment to achieve homeostasis. The heat shock response (HSR) is one of the most well studied fundamental cellular responses to environmental and physiological challenges, resulting in rapid synthesis of heat shock proteins (HSPs), which serve to protect cellular constituents from the deleterious effects of stress. In addition to its role in cytoprotection, the HSR also influences lifespan and is associated with a variety of human diseases including cancer, aging and neurodegenerative disorders. In most eukaryotes, the HSR is primarily mediated by the highly conserved transcription factor HSF1, which recognizes target …

Cellular Uptake Of Neutrohpil Elastase Links Inflammation To Adaptive Immunity, Elizabeth A. Mittendorf

Dissertations and Theses (Open Access)

Many tumors arise from sites of inflammation providing evidence that innate immunity is a critical component in the development and progression of cancer. Neutrophils are primary mediators of the innate immune response. Upon activation, an important function of neutrophils is release of an assortment of proteins from their granules including the serine protease neutrophil elastase (NE). The effect of NE on cancer has been attributed primarily to its ability to degrade the extracellular matrix thereby promoting invasion and metastasis. Recently, it was shown that NE could be taken up by lung cancer cells leading to degradation of insulin receptor substrate-1 …

Characterization Of A Novel Role For The To Usled- Like Kinase In Kinetochore Assembly And Function In Caenorhabditis Elegans, Jessica M. De Orbeta

Dissertations and Theses (Open Access)

Chromosome segregation is a critical step during cell division to avoid aneuploidy and promote proper organismal development. Correct sister chromatid positioning and separation during mitosis helps to achieve faithful transmission of genetic material to daughter cells. This prevents improper chromosome partitioning that can potentially result in extrachromosomal fragments, increasing the tumorigenic potential of the cells. The kinetochore is a protenaicious structure responsible for the initiation and orchestration of chromosome movement during mitosis. This highly conserved structure among eukaryotes is required for chromosome attachment to the mitotic spindle and failure to assemble the kinetochore results in aberrant chromosome segregation. Thus elucidating …

Tet1: A Unique Dna Demethylase For Maintenance Of Dna Methylation Pattern, Chunlei Jin

Dissertations and Theses (Open Access)

DNA methylation at the C5 position of cytosine (5-methylcytosine, 5mC) is a crucial epigenetic modification of the genome and has been implicated in numerous cellular processes in mammals, including embryonic development, transcription, X chromosome inactivation, genomic imprinting and chromatin structure. Like histone modifications, DNA methylation is also dynamic and reversible. However, in contrast to well defined DNA methyltransferases, the enzymes responsible for erasing DNA methylation still remain to be studied. The ten-eleven translocation family proteins (TET1/2/3) were recently identified as Fe(II)/2-oxoglutarate (2OG)-dependent 5mC dioxygenases, which consecutively convert 5mC into 5-hydroxymethylcytosine (5hmC), 5-formylcytosine and 5-carboxylcytosine both in vitro and in mammalian …

Immunological Mechanisms Of Extracorporeal Photopheresis In Cutaneous T Cell Lymphoma And Graft Versus Host Disease, Lisa Shiue

Dissertations and Theses (Open Access)

IMMUNOLOGICAL MECHANISMS OF EXTRACORPOREAL PHOTOPHERESIS IN CUTANEOUS T CELL LYMPHOMA AND GRAFT VERSUS HOST DISEASE

Publication No.___________

Lisa Harn-Ging Shiue, B.S.

Supervisory Professor: Madeleine Duvic, M.D.

Extracorporeal photopheresis (ECP) is an effective, low-risk immunomodulating therapy for leukemic cutaneous T cell lymphoma (L-CTCL) and graft versus host disease (GVHD), but whether the mechanism(s) of action in these two diseases is (are) identical or different is unclear. To determine the effects of ECP in vivo, we studied regulatory T cells (T-regs), cytotoxic T lymphocytes (CTLs), and dendritic cells (DCs) by immunofluorescence flow cytometry in 18 L-CTCL and 11 GVHD patients before …

Neural Responses To Food Pictures And Their Association With Dietary Intake, Edward Christenson

Theses and Dissertations

BACKGROUND: Food-related visual cues may affect eating behavior and energy intake. The purpose of this study was to determine the neural response to pictures of food and whether or not the neural responses were associated with energy intake. METHODS: Using a cross-sectional design, 60 adults participated in this study. Each participant reported to the laboratory in a fasted state, were fitted with a 128-electrode electroencephalogram (EEG) net, and were shown pictures grouped into three categories: high-calorie foods, low-calorie foods, and distractor pictures. These pictures were shown in random order. Furthermore, participants were shown these pictures in one passive condition and …

Analysis Of Genotype, Phenotype, And Age Progression In Phelan-Mcdermid Syndrome, Sara Sarasua

All Dissertations

Phelan-McDermid syndrome is a developmental disability syndrome associated with deletions of the terminal end of one copy of chromosome 22q13. The observed chromosomal aberrations include simple terminal deletions, interstitial deletions, deletions and duplications, and duplications without deletions. All patients have some degree of developmental disability and many also have hypotonia, autism, minor dysmorphic features, and seizures. I performed an epidemiological and cytogenetic investigation to better understand the etiology of Phelan-McDermid syndrome and to provide information to patients and their families, clinicians, and researchers investigating developmental disabilities. Deletions vary widely in size, from 60 kb to more than 9 Mb, but …

Molecular Genetics Of Nodule Number Regulation: Cloning, Characterization And Functional Studies Of The Root Determined Nodulator1 (Rdn1) Gene In Medicago Truncatula, Tessema Kassaw

All Dissertations

Nitrogen is critical to life. However, the majority of nitrogen on earth (in the atmosphere) is inert and unavailable to nearly all organisms. Metabolically diverse prokaryotes are the only organisms capable of fixing atmospheric nitrogen; rhizobia set up a symbiosis with legume plants allowing the plants to benefit from this ability. Since nodulation and the subsequent nitrogen fixation processes are energy intensive, the host plant must balance hosting of the rhizobia by limiting the number of nodules it forms through a mechanism called Autoregulation of Nodulation (AON). My study of mutants in the model legume Medicago truncatula defective in AON …

Analyses Of Consumer Perceptions, Farmer Challenges, Polyamine, And Amino Acid Content Of Organic And Conventional Fresh Produce Grown In South Carolina, Emily Steinberg

All Dissertations

Execution of the USDA organic standards led to more support for local food as distinct from organic food. The current study was conducted to examine fresh produce perceptions and purchasing decisions of S.C. consumers. Four-hundred and eight S.C. consumers were surveyed at S.C. grocery stores. More than one-third of the S.C. consumers not knowing about the 'certified S.C. grown' program suggest that the S.C. Department of Agriculture (SCDA) may want to improve the promotion of their program. Eighty-five percent of consumers indicated that they would choose local over organic. This information would be useful to local S.C. produce farmers who …

Gene Duplication And The Evolution Of Hemoglobin Isoform Differentiation In Birds, Michael T. Grispo

School of Biological Sciences: Dissertations, Theses, and Student Research

The majority of bird species co-express two functionally distinct hemoglobin (Hb) isoforms in definitive erythrocytes: HbA (the major adult Hb isoform, with α-chain subunits encoded by the α^A-globin gene) and HbD (the minor adult Hb isoform, with α-chain subunits encoded by the α^D-globin gene). The α^D-globin gene originated via tandem duplication of an embryonic α-like globin gene in the stem lineage of tetrapod vertebrates, which suggests the possibility that functional differentiation between the HbA and HbD isoforms may be attributable to a retained ancestral character state in HbD that harkens back to a primordial, …

Transcriptional Analysis Of Cervical Epithelial Cell Responses To Hiv-1, Andrew A. Block

School of Biological Sciences: Dissertations, Theses, and Student Research

Human Immunodeficiency Virus type 1 (HIV-1) infection causes a growing pandemic throughout the world, of which women comprise 51% of people who live with HIV-1, more than 60% in sub-Saharan Africa. HIV-1 infections of women are mainly acquired through female reproductive tract where cervical and vaginal epithelial cells are the first line of defense. Although HIV-1 does not directly infect epithelial cells, HIV-1 obligatorily interacts with and crosses over epithelial layer to infect susceptible target cells, mainly CD4+ T cells, in the lamina propria to initiate an infection. However, the mechanism and ramification of the interaction of HIV-1 and epithelial …

Fish Assemblage Structure In Black Hills, South Dakota Streams, Luke D. Schultz, Sarah J. Lewis, Katie Nicole Bertrand

The Prairie Naturalist

Understanding factors structuring fish assemblages in a particular area is valuable to both sport fishery management and native species conservation. Fish assemblages in the Black Hills are unique to South Dakota because they contain economically valuable introduced salmonids as well as native species of conservation need. Our objective was to examine the relationship between fish assemblages and geomorphic and reach-scale habitat features across multiple stream reaches in the Black Hills. Canonical correspondence analysis, a direct gradient ordination analysis, indicated that factors operating at multiple spatial scales interacted to structure fish assemblages. There also was indication of segregation between native species …

Creating Healthful Food Environments Through Policy Change: A Toolkit For Faith-Based Organizations, Marjorie Freedman

Faculty Publications

Creating Healthful Food Environments Through Policy Change: A Toolkit for Faith-Based Organizations provides practical information to members of the faith-based community for use when developing, adopting, and implementing a food and beverage policy within their organization. For the purposes of this Toolkit, the term “food and beverage policy” refers to a policy officially adopted by a faith-based organization (FBO) (e.g., a church, parish, temple, mosque) requiring that the food it purchases, provides, or sells to members and guests meets guidelines established by public health authorities. Such a policy might, for example, indicate the types and kinds and relative amounts of …

Intestinal Flow Of Microbial Protein And Rumen Undegradable Protein In Cattle Fed Corn Distillers’ Grains And Solubles, With Emphasis During Lactation, Ezequias Castillo Lopez

Department of Animal Science: Dissertations, Theses, and Student Research

Metabolizable protein (MP) is the true protein absorbed by the intestine, supplied by rumen undegradable protein (RUP) and microbial crude protein (MCP). This research was focused on evaluating the effect of dried distillers grains with solubles (DDGS) on the nature and supply of MP, with emphasis during lactation.

Experiment 1 evaluated the use of DNA markers and the effects of fermentation time and DDGS on rumen microbial growth

in vitro. Treatments were 1) CONTROL, 0% DDGS; 2) 20% DDGS replacing corn; 3) 20% DDGS replacing soybean meal and 4) 20% DDGS replacing corn and soybean meal. Results indicate that …

Effect Of Silicon On Plant Growth And Drought Stress Tolerance, Kaerlek W. Janislampi

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Silicone is a silicon-containing synthetic polymer. Silicon is a metalloid chemical element. Silicon is not considered an essential nutrient for plants, but it is typically abundant in soils and can be taken up in large amounts by plant roots. Silicon is known to have beneficial effects when added to the soil in which rice and several other plants are cultivated. These beneficial effects include disease and insect resistance, plant structural fortification, and regulation of the uptake of other plant nutrients. Silicic acid is the form of silicon in soils that is available to plants. In this study, the effect of …

Using Biophysical Geospatial And Remotely Sensed Data To Classify Ecological Sites And States, Carson A. Stam

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Within the Intermountain West, vast expanses of big sagebrush shrubland and steppe are considered emblems of the western range. Currently, there are approximately 60 million hectares of big sagebrush within the 11 western states, four million of which are in the state of Utah. However, the historic distribution of sagebrush has been impacted by conversion to other types of land cover through juniper encroachment, urbanization, invasive weeds, and agricultural expansion. In Utah alone, big sagebrush communities have been reduced to approximately 55% of their historic extent. A primary and current example of the cumulative impact of big sagebrush loss is …

Population Dynamics And Movements Of Translocated And Resident Greater Sage-Grouse On Anthro Mountain, Utah, Natasha W. Gruber

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Greater sage-grouse (Centrocercus urophasianus) populations have declined range-wide. Species translocations have been identified as a conservation strategy to augment declining populations in restored habitats. I evaluated protocols previously used to successfully translocate greater sage-grouse in Utah by comparing annual production, survival, habitat use, integration and seasonal movements of translocated birds and their chicks to the resident population. To conduct this study, I translocated 60 greater sagegrouse hens captured and radio-collared on Parker Mountain to Anthro Mountain in the spring of 2009 and 2010. I also captured and radio-collared 19 resident hens to serve as my control group. All …

Factors Influencing Relocation Success Of Utah Prairie Dog (Cynomys Parvidens), Rachel Curtis

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Utah prairie dogs have been extirpated in 90% of their historic range due to introduced disease, eradication, and habitat destruction. Most of the population lives on private land where animals burrow in lawns and agricultural fields, which keeps this threatened species continually in conflict with landowners. The Utah Division of Wildlife Resources has been relocating prairie dogs from private to public land since the 1970s, but relocations have been largely unsuccessful due to high mortality. Prairie dogs are highly social animals, but they are usually relocated without regard to their family group, or coterie. Coteries typically consist of one reproductive …

Characterisation Of Pharmaceutical Grade Horse Chestnut Waste, Joanna Przyborska

Theses

Horse chestnut residue was made available by a local pharmaceutical company. The residue was subjected to compositional analysis and studied to determine potential for residual biological activity, including prebiotic, antimicrobial, anti-hyaluronidase and anti-elastase effects.

The residue had 52 % total solids, including 2.97% ash, 6.93% protein, 1.89% crude fat. Oleic acid (74.7%) was the main fatty acid components in the crude lipid component. The major ash components were potassium, phosphorus, calcium and magnesium.

Prebiotic potential of aqueous extract (HCE) from horse chestnut residue was confirmed. A new formula for calculation of prebiotic index (PI) was developed. PI for probiotics (Lactobacillus …

Front Matter

The Synapse: Intercollegiate science magazine

No abstract provided.

Chemical Communication In The Honey Bee Scarab Pest Oplostomus Haroldi: Role Of (Z)-9-Pentacosene, Ayuka T. Fombong, Peter E.A. Teal, Richard T. Arbogast, Paul N. Ndegwa, Lucy W. Irungu, Baldwyn Torto

All Peer-Reviewed Publications

Oplostomus haroldi Witte belongs to a unique genus of afro-tropical scarabs that have associations with honey bee colonies, from which they derive vital nutrients. Although the attributes of the honey bee nest impose barriers to communication among nest invaders, this beetle still is able to detect conspecific mates for reproduction. Here, we show, through behavioral studies, that cuticular lipids serve as mate discrimination cues in this beetle. We observed five steps during mating: arrestment, alignment, mounting, and copulation, and a post-copulatory stage, lasting ~40-70 % of the total mating duration, that suggested mate guarding. Chemical analysis identified the same nine …

Taxonomy And Dna Barcoding In The Genus Manfreda (Salisb.) Asparagaceae, William David Ritchie

Graduate Theses and Dissertations

The genus Manfreda Salisb. of Asparagaceae is a genus of potential horticultural interest and is currently subject to breeding efforts at the University of Arkansas. A lack of taxonomic clarity however undermines the classification of potential inter - and intrageneric hybrids. The study aims to assess existing species delimitation within the genus Manfreda employing morphology while investigating the potential utility of Consortium for the Barcode of Life Plant (CBOL) DNA Barcodes for identification of specific taxa and an External Transcribed Spacer (ETS) - Internal Transcribed Spacer (ITS) DNA barcode for developed hybridized taxa.

Observation of 855 herbarium specimens facilitated phylogenetic …

Characterization Of Two Type Iv Collagens Involved In Autosomal Recessive Hereditary Nephropathy, Keri Tabb

All Dissertations

The glomerular basement membrane (GBM) of the kidney is highly specialized and required for filtration of waste products from the bloodstream. One of the major structural and functional units of the GBM is a network composed of type IV collagen heterotrimers, or protomers. Early in development of the human and dog, an isoform switch occurs, and the α1α1α2 (IV) network found in the nascent GBM is replaced with α3α4α5 (IV) protomers. Mutations in any of the genes encoding proteins found in the mature GBM network may prevent protomer formation and cause a progressive disorder leading to end stage renal failure …

Expansion Of Human Induced Pluripotent Stem Cells On Synthetic Substrate In Defined Medium, Huantong Yao

Graduate Theses and Dissertations

Human induced pluripotent stem cells (hiPSCs) have the potential to generate patient-specific cells to treat many incurable diseases by cell replacement therapy. However, so far the culture of hiPSCs depends greatly on feeder cells or Matrigel which has safety issues. Thus, chemically defined substrates that could provide niches necessary for cell attachment and proliferation are preferred for clinical application of hiPSCs. Recently, Corning Life Sciences has developed synthetic peptide-functionalized cell culture surface, referred to as Corning® Synthemax that support self-renewal and differentiation of human embryonic stem cell (hESC). In this work, we have collaborated with Corning to investigate the attachment, …

Response Of Broiler Chickens To Variation In Dietary Nutrient Content, Sarah Diane Goodgame

Graduate Theses and Dissertations

Rising feed costs, environmental pollution concerns targeted at animal agriculture, a worldwide focus on sustainability and the never ending battle to improve production and efficiency within the poultry industry challenges nutritionists to focus on products to improve nutrient digestibility and decrease feed costs while at the same time maximizing performance. The research presented in this dissertation focuses on improving nutrient utilization of protein sources with protein liberating enzymes currently available commercially. The research also conducted addresses the addition of crystalline amino acids valine and isoleucine and their effect on improving efficiency of broilers.

The first research project compared the efficacy …

Predictability Of Food And Wine Pairing Using A Sensory Approach, Rebeckah L. Koone

Graduate Theses and Dissertations

The purpose of this study was to explore significant differences in perception of match for a variety of food and wine styles; evaluate the impacts of wine sweetness level, wine acidity level, and wine tannin level on perception of match; and to investigate the impact of food and wine expertise on perceived level of match. A field research design was used to explore expert recommendations of food and wine combinations to obtain broader feedback on consumer perceptions. The population consisted of a convenience sample of 248 students enrolled in a continuing education course in food and wine pairing at George …

Effect Of Body Size And Exercise On Mood State, Alexandra Lachance

Graduate Theses and Dissertations

Exercising in the presence of nature (i.e., "green exercise"), has been shown to heighten the physiological and mental benefits of traditional exercise on non-obese individuals. The effects of green exercise on obese individuals are unknown. It is hypothesized that green exercise is more beneficial for obese (greater positive improvements in mental health scores), compared to non-obese individuals. For example, the benefits of green exercise could help lower feelings of depression, improve mood and self-esteem to increase the propensity of obese individuals to meet exercise recommendations. Accordingly, this study investigated the physiological and psychological effects of green exercise on 12 obese …

Meta-Analysis Of Wheat Qtl Regions Associated With Heat And Drought Stress, Marlovi Andrea Acuna Galindo

Graduate Theses and Dissertations

Heat and drought are the two most important environmental constraints to wheat production globally, are often present simultaneously and will become more severe with global climate change. This presents a unique challenge to wheat scientists who must work to develop wheat cultivars that are productive and adapted to future environmental conditions. A number of recent studies have reported quantitative trait loci (QTL) associated with heat and drought tolerance, as well as QTL for stress adaptive traits such as the availability of stem carbohydrates or crop canopy temperature. The objective of this study was to perform a meta-analysis of these QTL …

Evaluation Of The Recombination Efficiencies Of Flp Proteins, Linh Duy Nguyen

Graduate Theses and Dissertations

Site-specific recombination systems are powerful tools for genetic modification. They have been used to integrate a transgene into a pre-defined locus and to remove marker genes from a transgene locus. Two of the most widely used site-specific recombination systems in plants are the Cre/lox system from the bacteriophage P1 and the FLP/FRT system from the yeast Saccharomyces cerevisiae. The Cre/lox system is well-characterized and is the first choice in application of site-specific recombination system. However, some applications such as marker-free site-specific gene integration require the use of two recombination systems. In addition, the availability of alternative recombination systems can offer …

Long-Term Effects Of Rice Rotation, Tillage, And Fertility On Near-Surface Soil Carbon And Nitrogen Cycling, Jill Marie Motschenbacher

Graduate Theses and Dissertations

Rice (Oryza sativa L.)-based cropping systems are different from other row crops due to the flood-irrigation scheme used from about one month after planting to a few weeks prior to harvest. The frequent cycling between anaerobic (i.e., flooding during the growing season) and aerobic (i.e., generally, the remainder of the year) conditions can influence the rate of soil organic matter (SOM) decomposition, which can greatly influence carbon (C) and nitrogen (N) storage and sequestration in the soil over time. Therefore, a study was conducted on a silt-loam soil (fine, smectitic, thermic, Typic Albaqualf) at the Rice Research and Extension Center …