Life Sciences Commons^™

Adding Value To Graduate Education: The Comprehensive Examination, John L. Lindquist, Samuel E. Wortman, Charles A. Francis

Department of Agronomy and Horticulture: Faculty Publications

Virtually all graduate study requirements for MS or PhD degrees include a written comprehensive and an oral exam, the latter most often a presentation of thesis or dissertation results. The written exam takes many forms, but the goals are to test the candidate for technical competence and affirm that the prior program course work has been effective in bringing the candidate to an acceptable level of understanding of the discipline in which she or he has been immersed. Although long accepted as a useful hurdle on the path to a degree, for some exceptional students who have already demonstrated competence …

Design And Fabrication Of Nanofluidic Systems For Biomolecule Characterizations, Orain Ansel Hibbert

Graduate Theses and Dissertations

Nanofluidic channel systems were designed and fabricated by combining MEMS microfabrication with AFM nanolithography. In the fabrication process flow, photolithography was first utilized to pattern microfluidic channels and reservoirs on a 4" Pyrex substrate. Subsequently, atomic force microscopy (AFM) based nanolithography was used to mechanically fabricate nanochannels to connect the microreservoirs which formed the inlet and outlet of the nanofluidic system. A Tap190 Diamond-Like Carbon (DLC) AFM tip with a force constant of 48 N/m and a radius of less than 15 nm was used as the nanolithography tool. The resultant nanochannel ranges from 20 to 80 µm in length …

Subcritical Water Hydrolysis Of Whey Proteins, Ashley Dawn Espinoza

Graduate Theses and Dissertations

Hydrolyzing food by-products is a unique approach to potentially increase by-product value and reduce waste. An abundant by-product of cheese production, whey, contains all essential amino acids and some distinctive peptides with functional and nutraceutical properties. Typically, proteins from whey are tailored for specific uses by chemical or enzymatic hydrolysis; however, subcritical water hydrolysis is a novel alternative used successfully to hydrolyze various substrates. Nevertheless, minimal research exists on: (1) the hydrolysis of whey protein; (2) the incorporation of additives; (3) the hydrolysis of whole whey; and (4) the production of volatiles when using subcritical water hydrolysis.

Therefore, whey protein …

Rice (Oryza Sativa) Response To Low Glyphosate Rates As Influenced By Cultivar, Growth Stage, And Imazethapyr Applications, Jason R. Meier

Graduate Theses and Dissertations

Off-target movement of glyphosate onto rice is a perennial concern when rice is grown in close proximity to glyphosate-tolernat crops. If differential tolerance to sub-lethal rates of glyphosate exists among rice (Oryza sativa) cultivars, these cultivars could be utilized in breeding programs or glyphosate-drift sensitive areas. Field and greenhouse experiments were conducted in 2006 and 2007 to examine differences among rice cultivars in response to sub-lethal rates of glyphosate, and to examine imidazolinone-tolerant rice response to imazethapyr and sub-lethal rates of glyphosate applied sequentially to determine the potential for either herbicide to predispose rice to greater injury. In the field …

The Incidence Of Plexiform Lesion Formation In Lines Divergently Selected For Ascites, James Grant Mason

Graduate Theses and Dissertations

Complex Vascular Lesions, (CVL) are a common vascular change associated with idiopathicpulmonary arterial hypertension (IPAH). CVL have been characterized in studies using animal models involving, but not limited to, Sprague-Dawley rats and poultry (Abe et al., 2010; Wideman et al., 2011).IPAH has been documented in poultry although no connection has been made with CVLs. The current study characterizes CVL in the lungs from broilers derived from lines divergently selected for 15 generations for ascites susceptibility under conditions of simulated high altitude. The ascites RES and SUS lines were sampled for CVL incidence over time. Both lines were reared in a …

Protein Utilization And Requirements In Broiler Breeders, Ricardo David Ekmay

Graduate Theses and Dissertations

Advances in the genetics of poultry have created unintended consequences to the broiler breeder industry. Excessive weight has been shown to have many negative effects, including double hierarchies, multiple ovulations, and fatty liver. In an effort to curb excessive body weight gain, both the protein requirements and feeding strategies of broiler breeders are being revisited. A series of studies were conducted to determine the protein and amino acid requirements of broiler breeders during the production phase and to determine the interrelationship between protein turnover and egg formation. In the first trial, a 2x3x3 factorial study was conducted to determine the …

Effect Of Soyasaponin Fractions On Human Colon Cancer Cells, Joseph Tyler Salyer

Graduate Theses and Dissertations

Group B saponins, the predominant form of saponins in heat-treated soy products, have been shown to possess hypocholesterolemic, antimutagenic, and anticarcinogenic properties. Previous studies have evaluated crude mixtures of soyasaponins, but studies evaluating a single purified soyasaponin as an anticarcinogenic agent are limited. The goal of this study is to examine the effects of purified soyasaponins I and III as well as their aglycone form, soyasapogenol B, as anticarcinogenic agents on the human colon adenocarcinoma cell line Caco-2. Experiments were conducted to determine the effects of purified soyasaponins on cell proliferation, Protein Kinase C (PKC) activity, and cell morphology in …

Visualizing The Spatial Localization Of Active Matrix Metalloproteinases (Mmps) Using Maldi Imaging Ms, Sasirekha Muruganantham

Graduate Theses and Dissertations

Biomaterial implantation induces the foreign body response (FBR). Development of longer-term implants relies on the thorough understanding of the FBR. The progression of the FBR is regulated by a number of biomolecules including cytokines, chemokines, and matrix metalloproteinases (MMPs). The nature of the FBR requires the spatial and temporal regulation of these mediators. MMPs are an extremely large and diverse group of enzymes that play key roles in regulating the FBR. Precise spatiotemporal regulation of MMPs defines their proteolytic activities. The aim of this project is to develop a new bioanalytical method to visualize the localization of active MMPs at …

Hospitality Students Perceptions About High Risk Behaviors In Relation To Destructive Behaviors And Decision Making, Boris A. Roslov

Graduate Theses and Dissertations

This study investigated hospitality students' perceptions regarding alcohol consumption and personal actions related to high risk behaviors. Those behaviors included: sexual encounters and binge drinking, and their effect on academic tasks and performance. The study investigated whether the hospitality student who is employed full time consumes alcohol at a higher level than a hospitality student does that is not employed full time.

Adaptation And Validation Of Food Product Specific Analytical Methods For Monitoring Prebiotics Present In Different Types Of Processed Food Matrices, Rebbeca M. Duar

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Prebiotic carbohydrates are now added to a variety of processed foods to beneficially affect the gut microbial composition and activities. However, published data remain limited on the stability of prebiotics during food processes, such as baking, extrusion, pasteurization, high temperature heating, low pH condition, etc. As the complexity of the food matrix may also affect the ability to test for prebiotics, product specific analytical methods, including UV-vis spectroscopy, GC and HPLC, were developed and validated to monitor the stability of FOS, inulin and GOS in different types of processed food (breakfast cereal, cookie, muffin, sports drink and a nutritional bar). …

Infectious Disease And The Worldwide Distribution Of Iq, Christopher Guiles Eppig

Biology ETDs

We show that infectious disease is a major contributor to the worldwide distribution of human cognitive ability, as measured by psychometric IQ. In areas where infectious disease is high, average human intelligence tends to be lower, and in areas where infectious disease is low, average human intelligence tends to be higher. In separate studies, we tested this across world nations (chapter 2) and across states of the USA (chapter 3). In efforts to disseminate our research to wider audiences, I reviewed the findings contained in chapters 2 and 3 using language that is accessible to non-biologists (chapter 4). Although it …

Lateralization Of The Developing Rat Hippocampal Formation, Amanda Lynn Gross

Biology ETDs

Differences between the left and right hemisphere of the brain have been observed in humans and rodents (Broca, 1861; Wernicke, 1881), including the hippocampal formation, a region of the brain that is necessary for some forms of learning and memory (Olton and Samuelson, 1976; deToledo-Morrell et al., 1988; Bernasconi-Guastalla et al., 1994; Tabibnia et al., 1994; Poe et al., 2000; Lister et al., 2006; Hanlon et al., 2005; Sommer et al., 2005; Moskal et al., 2006; Thompson et al., 2008; Klur et al., 2009). Although lateralization of the hippocampal formation has been studied in the adult, few have sought to …

An Evaluation Of Coating Material Dependent Toxicity Of Silver Nanoparticles, Thilini Upekshika Silva

Electronic Theses and Dissertations

Silver nanoparticles (AgNPs) synthesized using numerous types of coating materials may exhibit different toxicity effects. The study evaluated coating material dependent toxicity by selecting 3 types of AgNP synthesis methods with different coating materials (citrate, polyvinyl pyrrolidone, and branched polyethyleneimine, coated AgNPs as citrate-AgNPs, PVP-AgNPs, and BPEI-AgNPs respectively). Two acute aquatic toxicity tests were performed; 48hr D. magna and MetPLATE E. coli toxicity tests. Significantly different toxicity effects were observed in D. magna test exhibiting lethal median concentrations (LC50) for citrate-AgNPs, PVP-AgNPs, and BPEI AgNPs respectively as, 2.7, 11.2, and 0.57μg/L. Median inhibitory concentrations (EC50) for MetPLATE tests were 1.27, …

Effect Of Pesticides On Salicylic Acid Binding Protein 2 (Sabp2) And Plant Defense, Joannes Petrus Yuh

Electronic Theses and Dissertations

Tobacco SABP2 has been shown to display high affinity for salicylic acid (SA) and methylsalicylate (MeSA) and plays an important role in SAR signal development. Using biochemical approach, SABP2 has been shown to demonstrate strong esterase activity in converting MeSA to SA. Recent study shows that tetra fluoroacetophenone, a synthetic analog of SA, competitively inhibits SABP2 esterase activity as well as suppresses SAR signal development in tobacco mosaic virus (TMV)-infected tobacco plants. Not much has been studied on the effect of pesticides on plant defenses. Because both AChE and SABP2 are esterase-like proteins belonging to α/β hydroxylase superfamily, we hypothesize …

Bumble Flower Beetle, Taun Beddes, Ryan S. Davis

All Current Publications

The bumble flower beetle (BFB) is a common member of the Scarabaeidae (scarab) beetle family. Its common name originated because adult BFB’s often fly close to the ground and emit a loud buzzing sound similar to that of a bumble bee. Larvae (grubs) and adults are often found in or near fermenting and/or decomposing organic matter.

Corn Earworm, Diane G. Alston, Shawn Olsen, James Barnhill

All Current Publications

In Utah, there are typically three generations of corn earworm (CEW) each year. The first generation of adults either come from overwintering pupae (southern and central Utah), or migrate into northern Utah. The adult moth is tannish brown with a 1 1/2 inch wingspan. The front wings are marked with a distinct dark spot in the center and darker bands near the outer margins. The hind wings are lighter tan, with a dark band along the outer margins. The male moths have green eyes. Moth flight occurs primarily on warm, overcast evenings. Moth populations can be monitored using pheromone or …

Hemipteran Beneficiosos: Damsel Bugs, Ricardo Ramirez, Ron Patterson, Forest Young

All Current Publications

Los insectos depredadores pueden proveer un control de pestes natural al comer insectos no deseados. Este servicio beneficioso se llama control biológico. Los insectos depredadores son una de las razones por las que las poblaciones de pestes pueden mantenerse a niveles no-dañinos.

Spruce Health In Utah Landscapes, Ryan S. Davis, Michael R. Kuhns, Claudia Nischwitz

All Current Publications

Spruces are common trees in cultivated landscapes in Utah. They have varied shapes, attractive foliage color, and can be fairly long-lived. They have pests, but not overly so, and are not very messy.

Getting Chemicals Into Trees Without Spraying, Michael R. Kuhns

All Current Publications

Chemicals are applied to trees for many reasons. Insecticides repel or kill damaging insects, fungicides treat or prevent fungal diseases, nutrients and plant growth regulators affect growth, and herbicides kill trees or prevent sprouting after tree removal. Spraying is the most typical way to apply these chemicals. It is fast, uses readily available equipment, and is understood. The down side of spraying is that much of the chemical being applied is wasted, either to drift, run off, or because it can not be applied precisely to where it is needed in the tree. Also, in many cases the chemical is …

Sunscald Injury Or Southwest Winter Injury On Deciduous Trees, Katie Wagner, Michael R. Kuhns

All Current Publications

Sunscald or southwest winter injury presents a significant threat to the trunks and limbs of certain deciduous trees. Although it can happen on shrubs it is more common on trees.

Fabrication Of Mineralized Collagen From Bovine Waste Materials By Hydrothermal Method As Promised Biomaterials, Faheem A. Sheikh, M. A. Kanjwal, Javier Macossay-Torres, Muneeb A. Muhammad, Travis Cantu, Ioannis S. Chronakis, N. A. M. Barakat, Hak Yong Kim

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Chemistry)

In the present study, we aimed to produce mineralized-collagen by hydrothermal process. A simple method not depending on additional foreign chemicals has been employed to isolate the mineralized-collagen fibers from bovine waste. The process of extraction involves the use of hydrothermal method from available bovine bones. The structural and morphological properties of the collagen fibers were characterized by using scanning electron microscopy and transmission electron microscopy. These results indicated well received collagen fibers, having a diameter less than 1 μm and with established mineral content in the individual fibers. The X-ray diffraction showed the crystalline feature of the obtained nano-compounds. …

Trematode Gill Parasite Infection In Brachyrhaphis Episcopi, Kara Million

Honors Capstone Projects and Theses

No abstract provided.

A Review Of Agronomic Management Strategies For Fusarium Head Blight. (Pre Print Version), Jasmine R. Hoover

Library Research and Publications

Develop a literature review and annotated bibliography of agronomic management practices that address FHB in wheat, barley and oats. Literature will focus on existing and emerging strategies that may have application in Western Canada. The review will summarize results according to topic and will include recommendations for future research and management approaches in Canada in addition to the annotated bibliography. The report will include an executive summary, introduction and sections on crop residue research, fungicide control research, bio-control research and any emerging management strategies. Additionally, the report will include a summary section and recommendations for applications in Canada and future …

The Nih-Niaid Filariasis Research Reagent Resource Center, Michelle L. Michalski, Kathryn G. Griffiths, Steven A. Williams, Ray M. Kaplan, Andrew Moorhead

Biological Sciences: Faculty Publications

Filarial worms cause a variety of tropical diseases in humans; however, they are difficult to study because they have complex life cycles that require arthropod intermediate hosts and mammalian definitive hosts. Research efforts in industrialized countries are further complicated by the fact that some filarial nematodes that cause disease in humans are restricted in host specificity to humans alone. This potentially makes the commitment to research difficult, expensive, and restrictive. Over 40 years ago, the United States National Institutes of Health–National Institute of Allergy and Infectious Diseases (NIH-NIAID) established a resource from which investigators could obtain various filarial parasite species …

Differential Combination Of Cytokine And Interferon- Gamma +874 T/A Polymorphisms Determines Disease Severity In Pulmonary Tuberculosis., Ambreen Ansari, Zahra Hasan, Ghaffar Dawood, Rabia Hussain

Department of Pathology and Laboratory Medicine

Background:Mycobacterium tuberculosis infects nearly 1/3 of the world population and this reservoir forms the largest pool from which new cases arise. Among the cytokines, IFN-gamma is a key determinant in protection against tuberculosis. Single nucleotide polymorphisms (SNPs) in IFN-gamma gene (+874 T/A) which determine TT high ((hi)), AA low ((lo)) and TA intermediate ((int)) responder phenotypes have shown variable associations with tuberculosis disease outcome in different ethnic populations. The objective of the current study was to analyze IFN-gamma gene combinations with other IFN-gamma regulating cytokine genes (IL-10, TNF -alpha, IL-6) to see the effect of gene- combinations on disease …

Store-Operated Calcium Channels In The Function Of Intracardiac Neurons, Timetria Bonds

USF Tampa Graduate Theses and Dissertations

Proper autonomic regulation of mammalian cardiac function is dependent upon very complex and precise communication among the intracardiac ganglia and individual neurons within the ganglia. An array of neuromodulators is found within the ganglia that direct neuronal activity by modulating the movement of calcium. The current study determines that opioidergic agonists, which have been found to contribute to severe cardiac disease states and intracellular calcium mobilization, are also responsible for changes in the function of the intracardiac neuron via their effects on store-operated calcium channels (SOCs).

Previous studies suggest that phosphorylation plays a role in SOC regulation. Using Fura-2 calcium …

Studies On The Adherence Properties Of Plant Lectins And Bacterial Adhesins And Their Inhibition By Prebiotic Oligosaccharides And Bovine Colostrum Fractions, Maria X. Maldonado-Gomez

Department of Food Science and Technology: Dissertations, Theses, and Student Research

To initiate colonization and infection, most microbial pathogens must first recognize and adhere to cells in the host tissues. Adherence inhibition, therefore, can be used as a strategy for preventing infectious disease. In this research, we developed a simplified model using plant lectins and HEp-2 tissue culture cells to simulate bacterial adhesins-receptors interactions. The effect of lectin ligands and two prebiotic carbohydrates, galactooligosaccharide (GOS) and polydextrose (PDX) was measured with this approach. Lectins that were able to bind to the target cells were inhibited in the presence of the cognate ligands and at least one of the prebiotic carbohydrates. The …

Dietary Fiber Consumption And Insulin Resistance: The Role Of Body Fat And Physical Activity, Charity B. Breneman

Theses and Dissertations

Background: This study was conducted to determine the association between fiber intake and insulin resistance in 264 women using a cross-sectional design. Methods: Insulin resistance was indexed using HOMA-IR (fasting insulin (μU/mL)×fasting glucose (mg/dL)/405). HOMA-IR values were log transformed. Fiber and energy consumption were assessed using 7-day weighed food records. Fiber was expressed as grams per 1000 kilocalories. Body fat percentage (BF%) was measured using the BOD POD and physical activity (PA) was ascertained using Actigraph accelerometers worn for 7 consecutive days. Results: (Mean±SD) age: 40.1±3.0 years, glucose: 86.7±5.9 mg/dL; insulin: 7.1±4.3 μU/mL; HOMA-IR: 1.5±1.0; fiber intake …

Smokejumper Obituary: Hanson, Philip Charles (Mccall 1951), National Smokejumper Association

Smokejumper Obituaries

No abstract provided.

Hiv-1 Tat Protein Promotes Neuronal Dysfunction Through Disruption Of Micrornas, J Robert Chang, Ruma Mukerjee, Asen Bagashev, Luis Del Valle, Tinatin Chabrashvili, Brian J. Hawkins, Johnny J. He, Bassel E. Sawaya

School of Medicine Faculty Publications

Over the last decade, small noncoding RNA molecules such as microRNAs (miRNAs) have emerged as critical regulators in the expression and function of eukaryotic genomes. It has been suggested that viral infections and neurological disease outcome may also be shaped by the influence of small RNAs. This has prompted us to suggest that HIV infection alters the endogenous miRNA expression patterns, thereby contributing to neuronal deregulation and AIDS dementia. Therefore, using primary cultures and neuronal cell lines, we examined the impact of a viral protein (HIV-1 Tat) on the expression of miRNAs due to its characteristic features such as release …