Life Sciences Commons^™

Daily Microgrowth Increments In Otoliths Of Common Dentex (Dentex Dentex Linneaus, 1758) Larvae Reared In Culture Conditions, Gülnur Meti̇n, Şahi̇n Saka, Kürşat Firat, Deni̇z Çoban

Turkish Journal of Veterinary & Animal Sciences

Daily microgrowth was determined in the otoliths of common dentex (Dentex dentex, Linneaus, 1758) larvae reared in 24 h illumination culture conditions. Microgrowth in otoliths was measured from the first mouth-opening stage of larvae on day 3 to the end of the larval stage on day 32. The sagitta and lapillus were present and were equal in size in newly hatched larvae, but the sagitta grew faster than the lapillus during further larval development. The first size increments in otoliths were observed at 4.25-4.99 mm total length of larvae (day 11). Structural development of the rostrum edge occurred on the …

The Cost-Benefit Analysis Of Early Weaned Piglets Fed With Different Protein Sources, Ferhan Kaygisiz, Fi̇li̇z Akdağ, Özkan Elmaz, Can Kutay

Turkish Journal of Veterinary & Animal Sciences

This study was carried out to analyse the economic effects of early weaning and of feeding with diets containing different protein sources in early weaned piglets. One hundred and fourteen piglets were used in this study. Fifty-four piglets were fed by suckling their mothers for 20 days and they weaned on day 21 and they were equally assigned to 2 experimental groups and 1 control group. Sixty piglets were fed by suckling their mothers for 27 days and they were weaned on day 28. These piglets were also assigned to 2 experimental groups and 1 control group. The first experimental …

Effects Of Atp-Mgcl_2 On Myocardial Ischemia-Reperfusion Injury: An In Vivo Experimental Study, Zülfi̇kar Kadi̇r Saritaş, Nusret Apaydin, Fatma Eser Özgenci̇l, Kamuran Pamuk, A. Tulga Ulus, Ayşen Aksoyek, Ufuk Tütün, Ali̇ İhsan Parlar, Sali̇h Fehmi̇ Katircioğlu

Turkish Journal of Veterinary & Animal Sciences

We investigated the effect of ATP-MgCl_2 on myocardial hemodynamics and metabolism. The study included 12 mongrel dogs that were randomly divided into 2 groups: the control and the ATP-MgCl_2 (study) groups. Following a right thoracotomy, the left anterior descending artery was ligated for 15 min and then reperfusion was performed. After the ischemic period, cardiopulmonary bypass was initiated and the aorta was cross-clamped. ATP-MgCl_2 (70+70 mmol/l) 0.25 ml/kg per h was given to the study group at the start of the ischemic period. Hemodynamic and metabolic measurements were obtained at basal, and then after 60, 120, and 180 min of …

Effect Of Eimeria Adenoeides Challenge Upon The Course Of An Experimental Salmonella Enteritidis Infection In Turkey Poults, Ventsislav Koinarski, Mihni Lyutskanov, Valentina Urumova

Turkish Journal of Veterinary & Animal Sciences

The aim of the present study was to perform a controlled experiment in order to evaluate the predisposing role of Eimeria adenoeides invasion on the appearance, development and severity of a Salmonella Enteritidis infection, with both infectious agents being field isolates obtained from spontaneous cases of the disease. Furthermore, we aimed to examine the principal epidemiological parameters of the mixed infection. The effect of an experimental Eimeria adenoeides invasion upon an artificial S. Enteritidis infection was studied in 160 turkey poults. The birds were divided into 4 experimental groups. Group 1 was infected with Eimeria adenoeides. Group 2 was infected …

Abortions In Sheep Due To Listeria Ivanovii In The Kars Region, Mi̇that Şahi̇n, Enver Beytut

Turkish Journal of Veterinary & Animal Sciences

This report describes an outbreak of Listeria ivanovii-induced abortion in sheep in the Kars region. In a flock of 120 sheep raised in Taşlıdere, a village in the region of Arpaçay, in Kars, some ewes in the last stages of gestation aborted in March, 2004. Necropsy was conducted on 5 aborted foetuses. Livers of the aborted foetuses showed diffuse necrotic foci. Histologically, the liver and lungs of aborted foetuses revealed diffuse coagulative necrosis infiltrated with macrophages and neutrophils. Bacterial culture from the liver and lungs of foetuses found pure growth of L. ivanovii subsp. ivanovii.

The Growth And Survival Characteristics Of Lambs Produced By Commercial Crossbreeding Kıvırcık Ewes With F_2 Rams With The German Black-Headed Mutton Genotype, Bülent Eki̇z, Ahmet Altinel

Turkish Journal of Veterinary & Animal Sciences

This study was conducted to compare the growth and survival characteristics of crossbred lambs produced by mating Kıvırcık ewes with German Black-Headed Mutton (GBM) x Kıvırcık (F_2) rams, GBM x Merino (F_2) rams, as well as the growth and survival characteristics of purebred Kıvırcık lambs. The data were obtained from 59 (GBM x Kıvırcık) x Kıvırcık, 59 (GBM x Merino) x Kıvırcık, and 60 purebred Kıvırcık lambs. The birth weights of (GBM x Kıvırcık) x Kıvırcık, (GBM x Merino) x Kıvırcık, and purebred Kıvırcık lambs were 4.08, 4.32, and 3.85 kg, respectively. The differences of birth weight between the genotype …

The Effect Of Age On Slaughter And Carcass Characteristics In Male Zavot Cattle, Ali̇ Riza Aksoy, Turgut Kirmizibayrak, Mustafa Saatci

Turkish Journal of Veterinary & Animal Sciences

This study was carried out to determine the effect of age on male Zavot cattle for slaughter and carcass characteristics. For this aim, 18 male Zavot cattle were divided equally into groups 1, 2 and 3 containing animals 6, 18 and 30 months old, respectively. The fattening period lasted 162 days and there was a 14-day adaptation period. Individual ad-libitum feeding was used during the experiment. At the end of the fattening period, the mean liveweight of groups 1, 2 and 3 was 202.9, 327.3 and 348.5 kg; hot carcass weight was 106.3, 190.7 and 197.2 kg; and the dressing …

\Beta-Lactoglobulin Variants In Awassi And Morkaraman Sheep And Their Association With The Composition And Rennet Clotting Time Of The Milk, Şerafetti̇n Çeli̇k, Sali̇h Özdemi̇r

Turkish Journal of Veterinary & Animal Sciences

In this study, \beta-lactoglobulin (\beta-Lg) variants in Awassi and Morkaraman sheep were studied using polyacrylamide gel electrophoresis, and their association with composition and rennet clotting time of the milk was estimated. Two alleles (A and B) and 3 different genotypes for \beta-Lg (AA, AB and BB) were observed in the whey from both breeds. The frequencies of \beta-Lg alleles were estimated as A 0.63 and B 0.37 in the Awassi breed, and A 0.56 and B 0.44 in the Morkaraman breed. These results indicate high \beta-A frequencies in both sheep breeds. Significant relationships were obtained between \beta-Lg variants and some …

Antigenic Diversity Of Bovine Viral Diarrhoea Viruses (Bvdv) Isolated In Turkey, Kadi̇r Yeşi̇lbağ, İbrahi̇m Burgu

Turkish Journal of Veterinary & Animal Sciences

Sixty BVDV field strains isolated from different regions of Turkey were antigenically characterised with a panel of 16 monoclonal antibodies (mAbs) specific to viral proteins E2 and NS2-3. MAb recognition patterns were detected by an indirect peroxidase-linked antibody assay and data were subjected to computer analysis for discussing herd specificity of viral strains isolated from immunotolerant, persistently infected (IPI) cattle. For that purpose, phylogenic and statistical analyses were performed. A high level of antigenic diversity was detected among the isolates. Diversity was also exhibited in IPI strains originating from the same herd. In some herds, viruses were divided into 2 …

Haematological And Coagulation Profiles During Severe Tropical Theileriosis In Cattle, Ramazan Çöl, Uğur Uslu

Turkish Journal of Veterinary & Animal Sciences

This study was conducted to measure selected haematological parameters in Holstein cattle naturally infected with Theileria annulata. Haematological analysis indicated significant decreases in red blood cell count, haematocrit value, haemoglobin amount, mean corpuscular haemoglobin concentration, and white blood cell, lymphocyte, neutrophil, monocyte, eosinophil, and basophil counts. On the other hand, significant increases were seen in mean corpuscular volume and marked reticulocytosis in infected animals compared to the animals in the control (uninfected) group. In the coagulation profile, activated partial thromboplastin time and prothrombin time were significantly prolonged, and platelet counts were significantly less in the infected group compared to those …

The Pathologic And Bacteriologic Comparison Of Pneumonia In Lambs, Ertan Oruç

Turkish Journal of Veterinary & Animal Sciences

The correlations between the bacteriologic agent of lamb pneumonia and its pathologic characters were investigated in this study. Pneumonia was detected in 262 (35.41%) of 740 diseased or dead lambs. Pathogenic bacteria were isolated in 114 cases (14.96% of all lamb deaths and 43.51% of all pneumonias). Lung lesions were histopathologically described as acute-catarrhal (17.56%), catarrhal-purulent (14.50%), purulent-necrotic (9.54%), fibrinous (26.72%), fibrinous-necrotic (5.73%), interstitial (18.32%), or verminous pneumonia (7.63%). In bacteriological studies, Mannheimia haemolytica (56.14%), E. coli (24.56%), and Pasteurella multocida (10.52%) were detected as important bacterial agents that caused different pneumonic lesions in lambs.

Serum Iron, Total Iron-Binding Capacity, Unbound Iron-Binding Capacity, Transferrin Saturation, Serum Copper, And Hematological Parameters In Pregnant Akkaraman Ewes Infected With Gastro-Intestinal Parasites, Süleyman Kozat, Nazmi̇ Yüksek, Yaşar Göz, İhsan Keleş

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to investigate serum iron parameters, and copper and iron deficiency anemia in ewes infected with gastro-intestinal parasites. This study was conducted with 60 pregnant Akkaraman ewes. In the statistical analyses, values obtained from both parasite infected and uninfected ewes were compared. Significant decreases were observed in PCV (P < 0.05), Hb (P < 0.05), RBC (P < 0.05), total protein (P < 0.05), albumin (P < 0.05), globulin (P < 0.05), and MCHC (P < 0.01), and a significant increase was seen in the WBC value (P < 0.05) in the parasite infected group. Furthermore, the parasite infected group had lower serum Fe (P < 0.001), TS % (P < 0.001), and serum Cu (P < 0.01) levels, and had higher UIBC values (P < 0.05) compared to the values obtain from the uninfected group. Therefore, in addition to antiparasite drug treatment in parasite infected pregnant ewes, iron preparations should also be administered in order to treat iron deficiency.

Sexually Dimorphic Social Development And Female Intrasexual Chemical Signaling Of African Elephants (Loxodonta Africana), Jordana M. Meyer

College of Graduate Studies: Theses & Dissertations

Author's abstract: African elephants are a polygynous species in which males and females carry out dimorphic lifestyles. Males search and compete for reproductively active females, while females care for offspring and facilitate group cohesion. The objectives of this study was a) to compare the development of sexually dimorphic behaviors and developmental trends between young male and female wild African elephants and b) to determine the ability of captive female African elephants to discern between the follicular and luteal phase of conspecifics through trunk-tip contacts and the investigation of urine, and whether the reproductive phase of the receiver affected the response …

Integrative Missing Value Estimation For Microarray Data, Jianjun Hu, H. Li, M. S. Waterman, X. J. Zhou

Faculty Publications

Background

Missing value estimation is an important preprocessing step in microarray analysis. Although several methods have been developed to solve this problem, their performance is unsatisfactory for datasets with high rates of missing data, high measurement noise, or limited numbers of samples. In fact, more than 80% of the time-series datasets in Stanford Microarray Database contain less than eight samples.

Results

We present the integrative Missing Value Estimation method (iMISS) by incorporating information from multiple reference microarray datasets to improve missing value estimation. For each gene with missing data, we derive a consistent neighbor-gene list by taking reference data sets …

New Localities For Leptotyphlops Macrorhynchus (Jan, 1862) (Reptilia: Leptotyphlopidae) In Turkey, İsmai̇l Hakki Uğurtaş, Murat Sevi̇nç, Mehmet Öz, Rahşen S. Kaya

Turkish Journal of Zoology

Four new localities of Leptotyphlops macrorhynchus (JAN, 1862) were recorded in Turkey. Two of the localities were found within the previously known distribution area on the east side of the Fırat River, and the other 2 were found on the west side. As a result, the distribution range of Leptotyphlops macrorhynchus has been extended. Information about the morphological features of the captured specimens is given.

Growth And Reproduction Of Por's Goatfish (Upeneus Pori Ben-Tuvia & Golani, 1989) In İskenderun Bay, The Eastern Mediterranean, Ali̇ İşmen

Turkish Journal of Zoology

The age composition, growth parameters, spawning season, sex ratio, length at first sexual maturity and fecundity of por's goatfish (Upeneus pori) caught from the Bay of İskenderun in the eastern Mediterranean Sea were investigated. Females and males made up 52.6% and 47.4%, respectively, of the particular population of the species. The total length of females ranged from 7.0 cm to 17.0 cm, and of males from 6.6 cm to 15.1 cm. The length (L) - weight (W) relationships for males and females were estimated as W = 0.0108*L^{2.99} and W = 0.0097*L^{3.03}, respectively. The age data derived from otolith readings …

Plants & Civilization; An Introduction To The Interrelationships Of Plants And People, James P. Smith Jr

Botanical Studies

No abstract provided.

A Position Paper On The Electronic Publication Of Nematode Taxonomic Manuscripts, Eyualem Abebe, James G. Baldwin, Byron Adams, Duane Hope, Scott Gardner, Robin Huettel, Peter Mullin, Thomas O. Powers, Jyotsna Sharma, Weimin Ye, William K. Thomas

Department of Plant Pathology: Faculty Publications

Several nematode species have now attained ‘model organism’ status, yet there remain many niches in basic biological inquiry for which nematodes would be ideal model systems of study. However, furthering the model system approach is hindered by lack of information on nematode biodiversity. The shortage of taxonomic resources to inventory and characterize biodiversity hinders research programs in invasion biology, ecosystem functioning, conservation biology, and many others. The disproportion between numbers of species to be described and numbers of available taxonomic specialists is greater for Nematoda than for any other metazoan phylum. A partial solution to the taxonomic impediment is the …

Wildlife And Invertebrate Response To Fuel Reduction Treatments In Dry Coniferous Forests Of The Western United States: A Synthesis, David S. Pilliod, Evelyn L. Bull, Jane L. Hayes, Barbara C. Wales

United States Department of Agriculture, Forest Service / University of Nebraska-Lincoln: Faculty Publications

This document is part of the Fuels Planning: Science Synthesis and Integration Project, a pilot project initiated by the USDA Forest Service to respond to the need for tools and information useful for planning site-specific fuel (vegetation) treatment projects. The information addresses fuel and forest conditions of the dry inland forests of the Western United States: those dominated by ponderosa pine, Douglas-fir, dry grand fir/white fir, and dry lodgepole pine potential vegetation types. Information was developed primarily for application at the stand level and is intended to be useful within this forest type regardless of ownership. Portions of the information …

Slow Ligand Binding Kinetics Dominate Ferrous Hexacoordinate Hemoglobin Reactivities And Reveal Differences Between Plants And Other Species, Benoit J. Smagghe, Gautam Sarath, Emily Ross, Jean-Louis Hilbert, Mark S. Hargrove

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Hexacoordinate hemoglobins are found in many living organisms ranging from prokaryotes to plants and animals. They are named “hexacoordinate” because of reversible coordination of the heme iron by a histidine side chain located in the heme pocket. This endogenous coordination competes with exogenous ligand binding and causes multiphasic relaxation time courses following rapid mixing or flash photolysis experiments. Previous rapid mixing studies have assumed a steady-state relationship between hexacoordination and exogenous ligand binding that does not correlate with observed time courses for binding. Here, we demonstrate that this assumption is not valid for some hexacoordinate hemoglobins, and that multiphasic time …

Characterization Of Hiv-1 Subtype C Envelope Glycoproteins From Perinatally Infected Children With Different Courses Of Disease, Hong Zhang, Federico Hoffmann, Jun He, Xiang He, Chipepo Kankasa, John T. West, Charles D. Mitchell, Ruth M. Ruprecht, Guillermo Orti, Charles Wood

Nebraska Center for Virology: Faculty Publications

Background: The causal mechanisms of differential disease progression in HIV-1 infected children remain poorly defined, and much of the accumulated knowledge comes from studies of subtype B infected individuals. The applicability of such findings to other subtypes, such as subtype C, remains to be substantiated. In this study, we longitudinally characterized the evolution of the Env V1–V5 region from seven subtype C HIV-1 perinatally infected children with different clinical outcomes. We investigated the possible influence of viral genotype and humoral immune response on disease progression in infants.

Results: Genetic analyses revealed that rapid progressors (infants that died in the first …

Design Clues From Functional Constraints And Broadly Neutralizing Antibodies, Tongqing Zhou, Ling Xu, Barna Dey, Ann J. Hessell, Shahzad Majeed, Donald Van Ryk, Shi-Hua Xiang, Xinzhen Yang, Mei-Yun Zhang, Michael B. Zwick, James Arthos, Dennis R. Burton, Dimiter S. Dimitrov, Joseph Sodroski, Richard Wyatt, Gary J. Nabel, Peter D. Kwong

Nebraska Center for Virology: Faculty Publications

No abstract provided.

Developing And Extending Sustainable Agriculture: A New Social Contract, Michele Schoeneberger, Gary Bentrup, Charles A. Francis, Richard Straight

United States Department of Agriculture, Forest Service / University of Nebraska-Lincoln: Faculty Publications

Conclusions

Based on the practical community applications of woody buffe rs and multifuncti onal areas that grew from the ini tial concepts ofecobelts. we are convinced that this type of initiative is a viable option in establishing a positive interface between different types of human activities. The challenge becomes more real every day, as cities expand and both city-edge subdivisions and acreages create ever more interfaces between people with different and often con!1icting activities and basic values. There is a need to provide some logical and acceptable boundary between these people and their different lifestyles. It is also desirable to …

Cross-Subtype T-Cell Immune Responses Induced By A Human Immunodeficiency Virus Type 1 Group M Consensus Env Immunogen, Eric A. Weaver, Zenaido T. Camacho, Fatiha Moukdar, Hua-Xin Liao, Ben-Jiang Ma, Mark Muldoon, James Theiler, Gary J. Nabel, Norman L. Letvin, Bette T. Korber, Beatrice H. Hahn, Barton F. Haynes, Feng Gao, Zhongjing Lu

Nebraska Center for Virology: Faculty Publications

The genetic diversity among globally circulating human immunodeficiency virus type 1 (HIV-1) strains is a serious challenge for HIV-1 vaccine design. We have generated a synthetic group M consensus env gene (CON6) for induction of cross-subtype immune responses and report here a comparative study of T-cell responses to this and natural strain env immunogens in a murine model. Three different strains of mice were immunized with CON6 as well as subtype A, B, or C env immunogens, using a DNA prime-recombinant vaccinia virus boost strategy. T-cell epitopes were mapped by gamma interferon enzyme-linked immunospot analysis using five overlapping Env peptide …

When Reintroductions Are Augmentations: The Genetic Legacy Of Fishers (Martes Pennanti) In Montana, Ray S. Vinkey, Michael K. Schwartz, Kevin S. Mckelvey, Kerry R. Foresman, Kristine L. Pilgrim, Brian J. Giddings, Eric C. Lofroth

United States Department of Agriculture, Forest Service / University of Nebraska-Lincoln: Faculty Publications

Fishers (Martes pennanti) were purportedly extirpated from Montana by 1930 and extant populations are assumed to be descended from translocated fishers. To determine the lineage of fisher populations, we sequenced 2 regions of the mitochondrial DNA genome from 207 tissue samples from British Columbia, Minnesota, Wisconsin, and Montana. In northwestern Montana, fishers share haplotypes with samples from the upper Midwest and British Columbia; in west-central Montana, we detected haplotypes found in British Columbia samples, but also detected a control region and cytochrome-b haplotype not found in source populations. Based on the unique haplotypes found in west-central Montana, …

Biotinylation Of K8 And K12 Co-Occurs With Acetylation And Mono-Methylation In Human Histone H4, Yap Ching Chew, Ashraf S. Raza, Gautam Sarath, Janos Zempleni

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Histones H1, H2A, H2B, H3, and H4 are proteins that are critical for folding of DNA into chromatin. Posttranslational acetylation, methylation, and biotinylation of histones participate in gene silencing, mitotic condensation of chromatin, and the cellular response to DNA damage. Various modifications of histones are known to interact ("cross-talk") in chromatin-remodeling events; interactions may be synergistic or antagonistic. Here, we sought to identify biotinylation sites in human histone H4 by using mass spectrometry (MS/MS), and we sought to determine whether biotinylation co-exists with acetylation and methylation in the same H4 molecule. Nuclear histones from human lymphoid (Jurkat) cells were digested …

Bullets On What's Happening At Unl Ianr, John Owens

John Owens: Speeches and Appearances

No abstract provided.

Distinguished Fellowship Awards Luncheon, John Owens

John Owens: Speeches and Appearances

It seems to me the best way to start off any congratulatory remarks is to say, quite simply - congratulations.

2006 East Campus Arbor Day Celebration, John Owens

John Owens: Speeches and Appearances

It cannot be lost on those of us present here today that Nebraska, the land of tall skies and wide prairies, where early settlers saw grass roll to the horizon broken only by the call of the lark, is the home of Arbor Day. Nebraska is, indeed, the tree planter state.

Agriculture Builders Of Nebraska, Inc. Annual Meeting, John Owens

John Owens: Speeches and Appearances

Those of us in the Institute of Agriculture and Natural Resources at your land-grant university view ourselves as partners with Nebraska. Taking the resources of this great university to the citizens of our state is a mission we take very, very seriously. We work hard to apply the university's resources in a diverse number of ways to benefit Nebraska. Today it is my very great pleasure to have this opportunity to provide a brief report to you, our partners, on some of the ways we are returning your investment in us to benefit our state.