Life Sciences Commons^™

Day 3: Friday, 19 August 2005: Habitat Conservation Plans, Susan Linner, Anne Ruggles, Anne Winans

Endangered Species Act Congressional Field Tour (August 17-19)

5 pages (includes illustration).

Contains references.

Red Rock Desert Learning Center: The Vision, Red Rock Desert Learning Center

Oliver Ranch Project

To instill stewardship and respect by increasing knowledge and understanding of the Mojave Desert ecosystems and cultures through a unique experiential discovery program.

Day 2: Thursday, 18 August 2005: Colorado Native Aquatic Species Restoration Program, Dave Schnoor

Endangered Species Act Congressional Field Tour (August 17-19)

6 pages (includes illustrations).

Contains one reference.

Day 2: Thursday, 18 August 2005: Upper Colorado River Endangered Fish Recovery Program, Bob Muth, Tom Pitts, Dan Luecke

Endangered Species Act Congressional Field Tour (August 17-19)

58 pages (includes illustrations and maps).

Contains references.

Day 2: Thursday, 18 August 2005: Candidate Conservation Agreements And Collaborative Multi-Party Agreements, Al Pfister, Gary Skiba, Tim Lehmann

Endangered Species Act Congressional Field Tour (August 17-19)

8 pages (includes illustrations and maps).

Contains references.

Day 2: Thursday, 18 August 2005: Canada Lynx Reintroduction, Gary Skiba, Rob Edward, Bonnie Kline

Endangered Species Act Congressional Field Tour (August 17-19)

5 pages (includes some color illustrations and maps).

Contains references.

Agenda: Endangered Species Act Congressional Field Tour, University Of Colorado Boulder. Natural Resources Law Center

Endangered Species Act Congressional Field Tour (August 17-19)

The Center sponsored its third annual field tour for staff members of the United States Congress, the United States Environmental Protection Agency, and the Colorado state legislature.

Day 1: Wednesday, 17 August 2005: Introduction, Mark Squillace

Endangered Species Act Congressional Field Tour (August 17-19)

5 pages.

Contains references.

Day 1: Wednesday, 17 August 2005: Science And The Esa, Joy Nicholopoulos, William Lewis

Endangered Species Act Congressional Field Tour (August 17-19)

43 pages (includes illustrations and map).

Contains references.

Day 1: Wednesday, 17 August 2005: Biodiversity And Critical Habitat, Charles Bedford, Federico Cheever, Tim Sullivan

Endangered Species Act Congressional Field Tour (August 17-19)

6 pages (includes color illustration).

Contains references.

Ua3/9/5 Conservation Reserve Enhancement Program (Crep), Wku President's Office

WKU Administration Documents

Talking points used by WKU president Gary Ransdell regarding WKU's partnering with Conservation Reserve Enhancement Program.

Src-Dependent Tyrosine Phosphorylation At The Tips Of Growth Cone Filopodia Promotes Extension, Estuardo Robles, Stephanie Woo, Timothy M. Gomez

Neuroscience: Faculty Publications

Extracellular cues guide axon outgrowth by activating intracellular signaling cascades that control the growth cone cytoskeleton. However, the spatial and temporal coordination of signaling intermediates remains essentially unknown. Live imaging of tyrosine phosphorylation in growth cones revealed dynamic phospho-tyrosine (PY) signals in filopodia that directly correlate with filopodial behavior. Local PY signals are generated at distal tips of filopodia during extension and are lost during retraction. Active Src family kinases localize to the tips of filopodia, and Src activity regulates both filopodial dynamics and local PY signaling. Positive guidance cues stimulate filopodial motility by locally increasing tyrosine phosphorylation in a …

Fatigue Resistance Of Rat Extraocular Muscles Does Not Depend On Creatine Kinase Activity, Colleen A. Mcmullen, Katrin Hayeß, Francisco H. Andrade

Physiology Faculty Publications

BACKGROUND: Creatine kinase (CK) links phosphocreatine, an energy storage system, to cellular ATPases. CK activity serves as a temporal and spatial buffer for ATP content, particularly in fast-twitch skeletal muscles. The extraocular muscles are notoriously fast and active, suggesting the need for efficient ATP buffering. This study tested the hypotheses that (1) CK isoform expression and activity in rat extraocular muscles would be higher, and (2) the resistance of these muscles to fatigue would depend on CK activity.

RESULTS: We found that mRNA and protein levels for cytosolic and mitochondrial CK isoforms were lower in the extraocular muscles than in …

Composition And Methods Of Using The Mirabilis Mosaic Caulimovirus Sub-Genomic Transcript (Sgt) Promoter For Plant Genetic Engineering, Indu B. Maiti, Nrisingha Dey

Kentucky Tobacco Research and Development Center Faculty Patents

The isolation of and methods of using a sub-genomic transcript (Sgt) promoter from Mirabilis mosaic virus (MMV) are described. A 333 bp MMV Sgt promoter fragment (sequence−306 to +27 from the transcription start site, TSS) was found to be sufficient for strongest promoter activity. This MMV Sgt promoter fragment shows comparable promoter activity to the MMV FLt promoter both in transgenic plants and in protoplasts. The MMV Sgt promoter also demonstrates much greater activity compared to Cauliflower mosaic virus (CaMV) 19S promoter and 35S promoter. The MMV Sgt promoter fragment and any chimeric gene to which it may be linked …

Isolation, Purification, And Chemical Characterization Of The Dihydroxamate-Type Siderophore, "Schizokinen," Produced By Rhizobium Leguminosarum Iari 917., Erin P. Storey

Electronic Theses and Dissertations

Iron is essential to the majority of microorganisms; it is an important cofactor in many cellular processes and enzymes. However in an aerobic environment and at biological pH, iron is primarily found as insoluble oxyhydroxides and is unavailable to microorganisms. Many bacteria have the ability to produce siderophores, low molecular weight compounds that have a high affinity for Fe³⁺. Siderophores are part of a multi-component system that actively transports the iron-siderophore complex into the cytoplasm. Rhizobia are characterized by their ability to form symbiotic relationships with leguminous plants, where they can fix nitrogen for the host plant and …

Effects Of The Implementation Of A Pilot Nutrition Education Program In A Rural Appalachian County., Leigh A. Davenport

Electronic Theses and Dissertations

The purpose of the study was to determine if nutrition knowledge would increase, indicated by improvements in pretest and posttest scores, following implementation of a nutrition lesson plan. The subjects included 532 fourth through eighth grade students from Johnson County, a rural county in northeast Tennessee. The lesson plans and pretests and posttests were developed to be appropriate for each grade level, with increased complexity in higher grades. The principal investigator graded the pretests and posttests and analyzed the data using SPSS. The results showed a significant improvement from pretests to posttests for all grades given a p-value of <0.05.

Red Rock Desert Learning Center Core Group Meeting: August 16, 2005, Red Rock Desert Learning Center

Reports (RRLC)

1. Introductions & Announcements (5 min.)
2. Approval of Minutes from July 19, 2005 Meeting (5 min.)
3. Matrix of Project Concerns and Resolutions – Michael Reiland (15 min.)
4. Update on Project Timeline Schedule – Michael Reiland (10 min.)
   A. Status of Operator Process
   B. Status of Water Options
   C. Status of Draft EA Report
5. Curriculum Development – Jeannie Klockow (10 min.)
   A. Wild Horse & Burro Curriculum
6. Standing Reports (20 minutes)
   A. Line and Space Architects – Les Wallach/Henry Tom
   B. BLM Capital Improvements – Michael Reiland
   C. Community Outreach – Nancy Flagg
7. Committee Reports (10 min.)
   A. Building Committee – Angie …

A Study Of The Relationship Of Self-Reported Resistance Training To Lipid Profiles., Melissa Davis

Electronic Theses and Dissertations

The primary purpose of this study was to determine the relationship of self-reported resistance training to lipid profiles. The study consisted of 10 subjects between the ages of 19 and 35. Participants were assigned to an exercise group or control group based on self-reported resistance training and according to ACSM standards. After means were determined for the data gathered, the exercise group had lower averages of weight, percent body fat, total cholesterol, low-density lipoprotein and triglycerides, as well as, a higher average of high-density lipoproteins. Two sample t-tests were performed to determine significance. It was determined that no significant difference …

C-Jun N-Terminal Kinase (Jnk) Is Required For Survival And Proliferation Of B-Lymphoma Cells, Murali Gururajan, Roger Chui, Anbu K. Karuppannan, Jiyuan Ke, C. Darrell Jennings, Subbarao Bondada

Microbiology, Immunology, and Molecular Genetics Faculty Publications

Several primary murine and human B lymphomas and cell lines were found to constitutively express high levels of the activated form of c-jun N-terminal kinase (JNK), a member of the mitogen-activated protein (MAP) kinase family. Proliferation of murine B lymphomas CH31, CH12.Lx, BKS-2, and WEHI-231 and the human B lymphomas BJAB, RAMOS, RAJI, OCI-Ly7, and OCI-Ly10 was strongly inhibited by SP600125, an anthrapyrazolone inhibitor of JNK, in a dose-dependent manner. The lymphoma cells underwent apoptosis and arrested at the G2/M phase of cell cycle. Furthermore, JNK-specific small interfering RNA (siRNA) inhibited the growth of both murine and human B lymphomas. …

Comparative Genetic Studies Of Native And Introduced Coccinellidae In North America, Elliot S. Krafsur, John J. Obrycki, James D. Harwood

Entomology Faculty Publications

During the past four decades, several species of aphidophagous Coccinellidae became established in North America, including Coccinella septempunctata, Harmonia axyridis, Hippodamia variegata, and Propylea quatuordecimpunctata. After their establishment, unknown circumstances favoured a rapid increase in population densities and distribution of H. axyridis and C. septempunctata at localities hundreds and thousands of kilometers from their release sites. Propylea quatuordecimpunctata and Hippodamia variegata have spread more slowly after becoming established in northeastern North America. Comparative studies based upon allozyme variation in these four introduced species and in six native North American species of ladybird beetles revealed …

Quantifying Aphid Predation Rates Of Generalist Predators In The Field, James D. Harwood, John J. Obrycki

Entomology Faculty Publications

The community of predators within agroecosystems has the potential to restrict aphid populations, especially early in the season before exponential increases in density and prior to the arrival of specialist natural enemies. Although direct observations of predation, laboratory feeding trials and manipulative field studies have been used to estimate levels of biological control exerted by different species (or potentially negative interactions between them), it is often difficult to extrapolate results to naturally occurring interactions in the field.

Over 100 investigations have utilized gut-content analysis to estimate aphid predation rates by predators. Throughout the last century, gut dissection, which enables the …

Vitex Agnus-Castus L., Gerry Moore

Specimens by Name

No abstract provided.

Role Of Spontaneous Current Oscillations During High-Efficiency Electrotransformation Of Thermophilic Anaerobes, Michael V. Tyurin, Charles R. Sullivan, Lee R. Lynd

Dartmouth Scholarship

Current oscillations at about 24 MHz were observed during electrotransformation (ET) of the thermophilic anaerobes Clostridium thermocellum ATCC 27405, C. thermocellum DSM 1313, and Thermoanaerobacterium saccharolyticum YS 485, using a pulse gated by a square signal generated by a custom generator. In experiments in which only the field strength was varied, all three of these strains resulted in a one-to-one correspondence between the appearance of current oscillations and successful ET. Oscillations accompanied ET of both C. thermocellum strains only at field strengths of >12 kV/cm, and ET was only observed above the same threshold. Similarly, for T. saccharolyticum, oscillations were …

Smokejumper Obituary: Davidson, Roger M. (Mccall 1947), National Smokejumper Association

Smokejumper Obituaries

No abstract provided.

Toward Sequencing The Sorghum Genome: A U.S. National Science Foundation-Sponsored Workshop Report, Stephen Kresovich, Brad Barbazuk, Joseph A. Bedell, Andrew Borrell, C. Robin Buell, John Burke, Sandra Clifton, Marie-Michéle Cordonnier-Ptratt, Stan Cox, Jeff Dahlberg, John Erpelding, Theresa M. Fulton, Bob Fulton, Lucinda Fulton, Alan R. Gingle, C. Tom Hash, Yinghua Huang, David Jordan, Patricia E. Klein, Robert R. Klein, Jurandir Magalhaes, Richard Mccombie, Paul Moore, John E. Mullet, Peggy Ozias-Akins, Andrew H. Paterson, Kay Porter, Lee Pratt, Bruce Roe, William Rooney, Patrick S. Schnable, David M. Stelly, Mitchell Tuinstra, Doreen Ware, Ujwala Warek

Faculty Publications

Members of the worldwide sorghum (Sorghum spp.) community, including private sector and international scientists as well as community representatives from closely related crops such as sugarcane (Saccharum spp.) and maize (Zea mays), met in St. Louis, Missouri, on November 9, 2004, to lay the groundwork for future advances in sorghum genomics and, in particular, to coordinate plans for sequencing of the sorghum genome. Key developments that made this workshop timely included advances in knowledge of the sorghum genome that provide for the development of a genetically anchored physical map to guide sequence assembly and annotation, the …

Interpretive Signs Designed To Trigger Naturalist Intelligence At Two American Zoos, Martha Bryant

Graduate Student Dissertations, Theses, Capstones, and Portfolios

An investigation of interpretive graphics was conducted in 2005 at two mid-sized AZA-accredited zoos, Lowry Park Zoo, Tampa, Florida and Knoxville Zoo, Knoxville, Tennessee. The Lowry Park Zoo study investigated signs at a red-tailed hawk and sandhill crane exhibit. Combination signs and wordless signs were more effective helping visitors see animals, increasing holding time, and number of engagements than treatments of no signs, or signs with words only. A second study, at Knoxde Zoo, tested combination and wordless signs in a children's zoo, investigating 31 signs at a 3.5-acre exhibit. Comparisons of visitors seeing the animals/using interactive exhibit elements, holding …

Goodyera Pubescens (Willd.) R. Br., Gordon C. Tucker

Specimens by Name

No abstract provided.

Viola L., Gordon C. Tucker

Specimens by Name

No abstract provided.

Structural And Molecular Characterization Of A Preferred Protein Interaction Surface On G Protein Beta Gamma Subunits, Tara L. Davis, Tabetha M. Bonacci, Stephen R. Sprang, Alan V. Smrcka

Biological Sciences Faculty Publications

G protein betagamma subunits associate with many binding partners in cellular signaling cascades. In previous work, we used random-peptide phage display screening to identify a diverse family of peptides that bound to a common surface on Gbetagamma subunits and blocked a subset of Gbetagamma effectors. Later studies showed that one of the peptides caused G protein activation through a novel Gbetagamma-dependent, nucleotide exchange-independent mechanism. Here we report the X-ray crystal structure of Gbeta(1)gamma(2) bound to this peptide, SIGK (SIGKAFKILGYPDYD), at 2.7 A resolution. SIGK forms a helical structure that binds the same face of Gbeta(1) as the switch II region …

Application Of A Multiple Testing Procedure Controlling The Proportion Of False Positives To Protein And Bacterial Data, Merrill D. Birkner, Alan E. Hubbard, Mark J. Van Der Laan

U.C. Berkeley Division of Biostatistics Working Paper Series

Simultaneously testing multiple hypotheses is important in high-dimensional biological studies. In these situations, one is often interested in controlling the Type-I error rate, such as the proportion of false positives to total rejections (TPPFP) at a specific level, alpha. This article will present an application of the E-Bayes/Bootstrap TPPFP procedure, presented in van der Laan et al. (2005), which controls the tail probability of the proportion of false positives (TPPFP), on two biological datasets. The two data applications include firstly, the application to a mass-spectrometry dataset of two leukemia subtypes, AML and ALL. The protein data measurements include intensity and …