Life Sciences Commons^™

Protostrongylus Stilesi (Nematoda: Protostrongylidae): Ecological Isolation And Putative Host-Switching Between Dall’S Sheep And Muskoxen In A Contact Zone, Eric P. Hoberg, Susan J. Kutz, John Nagy, Emily Jenkins, Brett Elkin, Marsha Branigan, Dorothy Cooley

Harold W. Manter Laboratory of Parasitology: Faculty Publications

The occurrence of Protostrongylus stilesi in a population of introduced muskoxen, Ovibos moschatus wardi, on the Arctic Coastal Plain, Yukon Territory (YT) and Northwest Territories (NT), Canada, is consistent with a contemporary colonization event from Dall’s sheep, Ovis dalli dalli, which indicates that host specificity may be ecologically based and contextual for this parasite. Colonization of muskoxen by P. stilesi may be a predictable event in zones of sympatry with Dall’s sheep; exposure to infection may coincide with occupation of winter ranges of Dall’s sheep by muskoxen during the summer season. Disruption of contemporary ecological isolating barriers can …

Foundations For An Integrative Parasitology: Collections, Archives, And Biodiversity Informatics, Eric P. Hoberg

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Burgeoning awareness about biodiversity emphasizes the fundamental importance of museum collections and the contributions of systematists and taxonomists in documenting the structure and history of the biosphere. An essential role is served by this infrastructure in collecting, preparing, analyzing, and disseminating information about the specimens that represent species, document a range of complex biological associations from symbioses to parasitism, and form the tapestry and the myriad facets of biodiversity (e.g., Wilson, 2000). As parasitologists we can examine how we may contribute to this broader documentation and understanding of global biodiversity, and we can articulate and communicate our role as vital …

Coccidia (Apicomplexa: Eimeriidae) Of The Mammalian Order Chiroptera, Donald W. Duszynski

Harold W. Manter Laboratory of Parasitology: Faculty Publications

The coccidia are protists (phylum Apicomplexa) that, likely, are both the most abundant (numbers of individual zoites) and most speciose of all the kinds of parasites found in/on mammals. They also are among the least studied and understood, with the exception of those species that cause pathology in domesticated Artiodactyla. In this review, I focus only on the largest family of the phylum, Eimeriidae Minchin, 1903, because its members often are among the most prevalent apicomplexans of mammals, and because there has never been a taxonomic summation for those species that infect Chirpotera. In all published descriptions of bat coccidia, …

Development Of The Muskox Lungworm, Umingmakstrongylus Pallikuukensis (Protostrongylidae), In Gastropods In The Arctic, Susan J. Kutz, Eric P. Hoberg, John Nishi, Lydden Polley

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Development of the muskox protostrongylid lungworm, Umingmakstrongylus pallikuukensis, in its slug intermediate host, Deroceras laeve, was investigated under field conditions in the Arctic. Every two weeks, from 19 June to 28 August 1997, groups of ten experimentally infected slugs were placed in tundra enclosures in a mesic sedge meadow near Kugluktuk, Nunavut, Canada. First-stage larvae (L1) infecting slugs on or before 17 July developed to third-stage larvae (L3) in 4–6 weeks. Intensity of L3 in slugs peaked at 6–8 weeks post infection (PI) and then progressively declined by 10, 12, and 48–50 weeks PI. Abundance of L3 in …

Detection Of Giardia Duodenalis Antigen In Coprolites Using A Commercially Available Enzyme-Linked Immunosorbent Assay, Marcelo Luiz Carvalho Gonçalves, Adauto Araújo, Rosemere Duarte, Joaquim Pereira Da Silva, Karl Reinhard, Françoise Bouchet, Luis Fernando Ferreira

Karl Reinhard Publications

The objective of this experiment was to assess the utility of a commercially available enzyme-linked immunosorbent assay (ELISA) kit for diagnosis of giardiasis in archaeological human remains. The kit, a monoclonal antibody assay, is used to detect the presence of Giardia-specific antigen 65 (GSA65) in human feces. We utilized the assay in ancient fecal material. The material included desiccated feces found in mummies or in archaeological sites, and sediments from latrines. A total of 83 specimens, previously examined microscopically for parasites, were examined. The ELISA detected 3 positive samples, dated to about 1200 AD, 1600 AD, and 1700 AD. …

Multidisciplinary Coprolite Analysis, Karl Reinhard, Dennis R. Danielson, Mark Daniels, Sérgio Augusto De Miranda Chaves

Karl Reinhard Publications

Coprolite analysis, as reviewed by Reinhard and Bryant (1992), contributes unique and detailed information regarding diet and parasitic disease. We present here an analysis of dietary components of coprolites from Bighorn Cave using macroscopic remains, pollen concentrations, and phytoliths. In addition, we analyzed Bighorn Cave coprolites for evidence of parasitic organisms, especially intestinal worms. Such analyses of coprolites have become important methods for reconstructing past dietary and medicinal practices. Pollen concentration and phytolith quantification techniques have recently been developed, but until this report no known attempt has been made to synthesize pollen, macroscopic, and phytolith data from a single coprolite …

Ultrastructure Of Tuzetia Weidneri Sp. N. (Microsporidia: Tuzetiidae) In Skeletal Muscle Of Litopenaeus Setiferus And Farfantepenaeus Aztecus (Crustacea: Decapoda) And New Data On Perezia Nelsoni (Microsporidia: Pereziidae) In L. Setiferus, Elizabeth U. Canning, Alan Curry, Robin M. Overstreet

Harold W. Manter Laboratory of Parasitology: Faculty Publications

A new microsporidian species, Tuzetia weidneri sp. n. , is described from the skeletal muscle of the decapod crustaceans Litopenaeus setiferus and Farfantepenaeus aztecus. Fresh spores are pyriform, measuring 3.1 x 2.3 µm. All stages have unpaired nuclei. Meronts lie in direct contact with degenerate host cell cytoplasm but produce numerous small blisters at the surface. Multinucleate meronts divide by constriction into groups or chains of uninucleate products. Sporogony is initiated by deposition of a dense surface coat on the plasma membrane of uninucleate or multinucleate stages and fusion of blisters to enclose the sporont in a sporophorous vesicle …

Potential Pathological Effects Of Blood Flukes (Digenea: Sanguinicolidae) On Pen-Reared Marine Fishes, Stephen A. Bullard, Robin M. Overstreet

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Sanguinicolids, or fish blood flukes, infect the vascular system of both marine and freshwater fishes, and some act as serious pathogens of hosts in aquaculture. Blood flukes typically possess a relatively benign relationship with wild fishes; however, cultured hosts near appropriate intermediate hosts (i.e., snail, bivalve, or polychaete) may accumulate heavy infections of the worms and their eggs. The resulting disease, sanguinicoliasis, has caused mass mortalities of fish reared in ponds and cages in North America, Europe, and Asia. In the life cycle, the cercaria emerges from the intermediate invertebrate host and penetrates into and matures in the definitive fish …

Hydrobiid Snails (Mollusca: Gastropoda: Rissooidea) From St. Andrew Bay, Florida, Richard W. Heard, Robin M. Overstreet, John M. Foster

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Heleobops sp. of Hershler and Thompson appears to be the only previously published record for the gastropod family Hydrobiidae Troschel in tidal waters of the St. Andrew Bay System, Florida. Six species occurred in bayous, marshes, and brackish ponds associated with the System during studies conducted between 1984 and 1999: Texadina barretti (Morrison), Texadina sphinctostoma Abbott and Ladd, Littoridinops monroensis (Frauenfeld), Littoridinops palustris Thompson, Onobops jacksoni (Bartsch), and Heleobops sp. A. The last is an apparently undescribed species closely related to Heleobops carrikeri Davis and McKee. Based in part on this study, Heleobops sp. A, which occurs in brackish habitats …

Declared Plant Control Handbook Recommendations For The Control Of Declared Plants In Western Australia, John R. Peirce, Rachael A. Pratt

Biosecurity published reports

Sixth Edition.

Reviewed October 2005.

Plants may be "declared" by the Agriculture Protection Board under the Agriculture and Related Resources Protection Act 1976.If a plant is declared, all landholders are obliged to control that plant on their properties. Declarations specifies a category, or categories, for each plant according to the control strategies or objectives which the Agriculture Protection Board believes are appropriate in a particular place.

Among the factors considered in categorising declared plants are:

* the impact of the plant on individuals, agricultural production and the community in general,

* whether it is already established in the area, and …

In Vitro Propagation Of Conradina Etonia, Cheryl L. Peterson, Russell C. Weigel

Ocean Engineering and Marine Sciences Faculty Publications

Conservation institutions worldwide such as the Royal Botanical Gardens at Kew (U.K.) have frequently used in vitro propagation (micropropagation) as a valuable tool to support the conservation of some threatened and endangered species. Although many of the endemic species of Florida 's scrub ecosystem are extremely rare, they have not been widely researched using tissue culture techniques. In this paper, the amenability of Conradina etonia, an endangered scrub mint species, to in vitro growth and propagation was investigated using shoot tips. Callus formed and grew rapidly in the presence of both cytokinins and auxins. Rhizogenesis from callus occurred in both …

Parallelism In Digging Behaviour In Two Distantly Related Decapod Crustaceans, Zen Faulkes

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

Walking and swimming are the most common forms of locomotion used routinely by decapod crustaceans, but some have evolved ways of locomoting throughsubstrates, notably sand. Two such digging species, of different infraorders, are spanner crabs (Ranina ranina; Brachyura) and spiny sand crabs (Blepharipoda occidentalis; Anomura). Videotape and electromyogram analysis showed that digging by these species is similar, but Raninahas a wider locomotor repertoire than Blepharipoda: for instance, Raninacan walk forward on the benthos, whereas Blepharipoda(and other sand crabs) cannot walk. Similarly, although both Raninaand Blepharipodadig into the sand using their legs and abdomen, leg movements of Raninaare less stereotyped than …

Genetic Variation For Fusarium Head Blight Resistance In Soft Red Winter Wheat, Marla Dale Hall

University of Kentucky Master's Theses

Fusarium graminearum, the causative agent of Fusarium head blight, is an economically important pathogen of wheat (Triticum aestivum). Breeding Fusarium head blight (FHB) resistant wheat requires knowledge of the underlying genetic control of FHB resistance.

Two nine-parent diallel analyses were completed in greenhouse and field environments. Combining abilities, variance component ratios, and narrow sense heritabilities for FHB resistance and deoxynivalenol levels were calculated. Significant general and specific combining ability effects were observed. Resistance to FHB seems to be mostly controlled by additive genetic effects with some dominance noted in the field. Resistance noted in the greenhouse environment …

Effects Of A Methanol Extract Of Eurycoma Longifolia On The Uptake And Release Of Dopamine In Rat Brain Synaptosomes, Lee Ning Lai

Student Works (2000-2009)

The effects of the crude alkaloid fraction of Eurycoma longifolia root on the release and uptake of dopamine was Investigated in vitro using rat brain synaptosomes. Synaptosomes were pr&-loaded with [3H]dopamine and then incubated with the crude alkaloid fraction dissolved in Krebs bicarbonate buffer. Pr&-loaded synaptosomes incubated with Krebs bicarbonate buffer alone served as controls. The amount of radioactivity released into the incubation medium was measured by liquid scintillation counting. To investigate the effect of the crude alkaloid fraction on the uptake of dopamine, synaptosomes were incubated with ['H]dopamine in the presence or absence of the crude alkaloid fraction. The …

The Effects Of Air Sparging On Ultrafiltration Of Whey, Ching Soon Tan

Student Works (2000-2009)

This work concerns the use of gas-liquid two-phase flow to reduce whey proteins and particle membrane fouling in hollow fibre dialyzer by injecting air directly into the whey feed stream. The effects of air bubbles on the permeate flux of the air sparged ultrafiltration system were studied experimentally. In comparison to single phase flow, over the range of operating conditions studied, the gas-liquid two-phase flow increases the ultrafiltration flux by 15% to 87%. The highest gas injection ratio Fgas/(Fliq +Fgas) was found to be in the range of 0.4 to 0.5. Within this range, the bulk concentration of whey proteins …

Forage Yield Of Different Alfalfa Cultivars Under Ankara Conditions, Suzan Altinok, Azi̇z Karakaya

Turkish Journal of Agriculture and Forestry

This research was carried out in the experimental field of Ankara University, Faculty of Agriculture, Department of Field Crops during the years 1997, 1998, and 1999. Alfalfa cultivars Elçi, Kayseri, Mesa Sirsa, Fortress, Bilensoy-80, 5638/Miral, Peru, and Bitlis were used as materials. Plant heights of alfalfa cultivars during three years were highest before first cuts, and the highest forage yields were obtained from these cuts. According to the results, total green yields and dry matter yields were 1869 and 651 kg/da in first year, 4071 and 1297 kg/da in the second year, and 3839 and 1226 kg/da in the third …

Amelioration Of Chilling Injuries In Watermelon Seedlings By Abscisic Acid, Ahmet Korkmaz

Turkish Journal of Agriculture and Forestry

A greenhouse study, designed in a randomized complete block design with five replications, was carried out at Clemson University, Clemson, SC, USA, in the spring of 1997. The objective of the study was to investigate whether abscisic acid (ABA) would mitigate chilling damages in the watermelon, a chilling-sensitive plant. 'Crimson Sweet' [Citrullus lanatus (Thumb) Matsum. & Nakai.] watermelon seedlings were grown in a greenhouse with a temperature regime of 25ºC (day) and 20ºC (night). Five-day-old seedlings were sprayed with 10^{-4} M cis, trans-abscisic acid 15 hours prior to being exposed to 1.5 ± 0.5ºC for 24 hours in a dark …

Effects Of Seed Origin And Site On Wood Density Of Sitka Spruce (Picea Sitchensis (Bong.) Carr.) Grown In Britain, İlker Usta, Mike Hale

Turkish Journal of Agriculture and Forestry

Since density may have a direct influence on wood properties and because density is a factor under genetic control, this study details the effects of seed origins and sites on density. In addition to seed origins, its variability both within and between trees was also examined. In this case, the variation in density was investigated in eight seed origins of Sitka spruce (Alaska-AL, British Columbia-BC, Queen Charlotte Islands-QCI, North Washington-NW, South Washington-SW, North Oregon-NO, South Oregon-SO, and California-CA) grown at two sites in Britain (Dalby: Eastern England and Rhondda: South Wales). For this purpose, the five trees of each seed …

Factors Affecting The Production Of E1 Type Particleboard, Gökay Nemli̇

Turkish Journal of Agriculture and Forestry

This study aimed to determine the best manufacturing variables for the high physical and mechanical properties, and formaldehyde emission of particleboards produced from E_1 type adhesive. In the production of particleboards, two different press temperatures, press times, pressures and three different adhesive use ratios were used. The best manufacturing variables were determined for the particleboard produced from E_1 type adhesive. The effects of these manufacturing variables on formaldehyde emission, bending strength, internal bonding strength and thickness swelling were investigated. According to the results, when the E_1 type adhesive was employed, the use of 11% and 9% adhesive use ratios for …

Hydraulic Conductivity Evaluation For A Drainage Simulation Model (Drainmod), Teki̇n Özteki̇n

Turkish Journal of Agriculture and Forestry

The objective of this study was to determine the most suitable saturated hydraulic conductivity (K) values as input into DRAINMOD among a range of K data sets developed for the drainage experiment site in North Central Ohio using seven different K estimation methods. The K methods evaluated were monolith, core, auger-hole, MUUF soil database, and using the Hooghoudt, Kirkham, and van Schilfgaarde equations with water table drawdown and drainage discharge data. Simulations using DRAINMOD (Version 4.6) were conducted for the years 1962-1964 and 1967-1971, and runoff and drain discharge predictions were compared with measured discharges. The analyses showed that none …

Estimation Of Instantaneous Peak Flows In Seyhan River Basin Using Regional Regression Procedures, Fati̇h Topaloğlu

Turkish Journal of Agriculture and Forestry

Multivariate procedures for estimating instantaneous flood flows for various return periods in Seyhan river basin are presented in the study. Procedures aim at developing regression equations uscable for the basin using annual peak instantaneous flows, climatic and morphometric charactcristics. It was determined that best subset analysis gave the highest determination coefficients (91.0%

An Estimation Of The Recreational Use Value Of Kursunlu Waterfall Nature Park By The Individual Travel Cost Method, Veli̇ Ortaçeşme, Burhan Özkan, Osman Karagüzel

Turkish Journal of Agriculture and Forestry

The recreational use value of Kursunlu Waterfall Nature Park in the Antalya province of Turkey was studied by using the Individual Travel Cost Method (ITCM). For this purpose, 500 on-site questionnaires were administered between September 1998 and June 1999. By applying certain criteria to these questionnaires, 280 cases were selected for economic analysis. The number of visits made by individuals was used as the dependent variable, while travel costs of individuals to the Park, socio-economic variables (age, education and household income) and alternative sites were selected as the independent variables in the demand model of the Park. A semi-log functional …

Sampling Sunn Pest (Eurygaster Integriceps Puton) In Overwintering Sites In Northern Syria, Bruce L. Parker, Scott D. Costa, Margaret Skinner, Mustapha El Bouhssini

Turkish Journal of Agriculture and Forestry

Sampling statistics for development of a sampling plan for adult Sunn Pest, Eurygaster integriceps Puton, in their overwintering habitat were generated based on numbers of live and total adults in 1 to 4 litter samples per tree. Taylor's power law was used to estimate optimum sample size, critical stop lines for sequential sampling and a sequential difference test. Sunn Pest were found primarily nearer to tree boles and had an aggregated distribution among trees. There was more statistical and practical advantage to taking 1 sample per tree and increasing the number of trees sampled, rather than in increasing the number …

Development, Survival And Reproduction Of Three Coccinellids Feeding On Hyalopterus Pruni (Geoffer) (Homoptera: Aphididae), Remzi̇ Atlihan, Mehmet Bora Kaydan

Turkish Journal of Agriculture and Forestry

The development, survival and fecundity of three aphidophagus coccinellid species, Scymnus apetzi (Mulsant), Scymnus subvillosus (Goeze) and Exochomus nigromaculatus (Goeze), were studied under laboratory conditions (25 ± 1, 65 ± 5% RH and 16 L/8D). Development time from egg to adult was 20.4, 17.1 and 16.7 days for S. apetzi, S. subvillosus and E. nigromaculatus, respectively. Mortality rate from egg to adulthood was highest for S. apetzi (37.9%) followed by S. subvillosus (36.3%) and E. nigromaculatus (25.7%). Duration of the oviposition period was 58.6, 64.9 and 75.3 days, and the total number of eggs per female was 492.8, 224.9 and …

Microscopic Evaluation Of Interactions Between Varieties Of Arabidopsis Thaliana Challenged By Peronospora Parasitica, Fi̇gen Mert Türk

Turkish Journal of Agriculture and Forestry

Peronospora parasitica (Pers ex Fr.) Pers. is an obligate biotrophic pathogen that causes downy mildew in Arabidopsis thaliana (L.) Heynh. In this study, cotyledons of four A. thaliana varieties were inoculated with the Cala2 isolate of P. parasitica and the degree of susceptibility was observed under the microscope 1, 2, 3 and 7 days after inoculation (DAI). Microscopic examination of infected tissues revealed that early restriction of the pathogen was accompanied by a hypersensitive response (HR) characterised by autofluorescence under UV or blue light excitation. All haustoria, if visible at all, formed in the cells that underwent the HR in …

Root Yield And Quality Of Sugarbeet In Relation To Sowing Date, Plant Population And Harvesting Date Interactions, Ramazan Çakmakçi, Erol Oral

Turkish Journal of Agriculture and Forestry

Sowing date, plant density and harvest date are important factors affecting root yield and quality of sugar beet (Beta vulgaris L.). The effects of sowing date (beginning of April, mid-April, the end of April, mid-May, and the end of May), plant density, and harvest date (the end of September and mid-October) on sugar beet yield and quality were studied in trials in the Eastern Anatolia region on a loam soil in 1994-97. Variation in plant density was obtained by combining two seed distances and two target emergence percentages. Plant populations for the different treatments reached 55 500, 73 000, 88 …

Kinetic Studies With Crude Tomato Alcohol Dehydrogenase, Emi̇n Yilmaz

Turkish Journal of Agriculture and Forestry

Tomato alcohol dehydrogenase (ADH) is very important in the formation of fresh tomato aroma volatiles. The kinetic characterization and isolation of this enzyme may promote some in vitro applications. Kinetic studies of crude tomato ADH associated with NAD^+ cofactor revealed Km values of 2.03 mM for ethanol and 0.064 mM for NAD^+, a Vmax value of 24.51 µM/min, and an activation energy of 0.0208 kcal/mole. Variable pH measurements indicated that the enzyme is optimally active in a narrow range above pH 8.5. Dalziel coefficients were consistent with a sequential but not ordered mechanism. Also the enzyme was found to be …

Principal Mechanical Properties Of Eastern Beech Wood (Fagus Orientalis Lipsky) Naturally Grown In Andırın Northeastern Mediterranean Region Of Turkey, İbrahi̇m Bektaş, Cengi̇z Güler, M. Altay Baştürk

Turkish Journal of Agriculture and Forestry

The aim of this study was to determine some mechanical properties (compression, static bending, impact bending and shear strengths) of Eastern beech wood (Fagus orientalis Lipsky) and to compare with them other beech species. In the tests, randomly selected logs taken from the trunk 2-4 m height were obtained from trees naturally growing in Andırın (Northeastern Mediterranean region) and these logs were prepared and tested according to Turkish standards. The results showed that the mean compression strength was 606 kgcm^{-2}, static bending strength 1204 kgcm^{-2}, impact bending strength 0.85 kgmcm^{-2} and shear strength 99 kgcm^{-2}. The relations of strengths with …

Satellite Monitoring Of Land Cover / Land Use Change Over 15 Years And Its Impact On The Environment In Gebze / Kocaeli - Turkey, Hülya Yildirim, Mehmet Emi̇n Özel, Nalan Jale Di̇van, Alparslan Akça

Turkish Journal of Agriculture and Forestry

The rapid industrialisation of an area requires quick preparation of actual land-cover/land-use (LC/LU) maps in order to detect and avoid overuse and damaging of the landscape beyond sustainable development limits. Satellite Remote Sensing and Geographic Information System technologies fit very well for long-term monitoring and assessment of such effects. As a pilot area for the use of such technologies, Gebze district near metropolitan Istanbul is considered. The study is centred on the documentation of LC/LU changes resulting from fast and mostly unregulated industrialisation and urbanisation over the period 1986 - 1998 by satellite images of the area. A comparison is …

Irrigation Scheduling Of Drip-Irrigated Tomatoes Using Class A Pan Evaporation, Öner Çeti̇n, Osman Yildirim, Demet Uygan, Hasan Boyaci

Turkish Journal of Agriculture and Forestry

This study was carried out to investigate the irrigation schedule of drip-irrigated tomatoes (Lycopersicon esculentum cv. Dual Large, F 1) using Class A pan evaporation. Tomatoes plants were grown in a clay soil in the experimental fields of the Research Institute of Rural Services in Eskişehir between 1998 and 2000. Irrigation water was applied as a certain ratio of Class A pan evaporation (k_{pc} = 0.50, 0.75, 1.00, and 1.25) with different irrigation intervals (2, 4, and 6 days). Significant differences in fruit yields were obtained between the treatments, except for in 1998. Maximum marketable fruit yield was found at …