Life Sciences Commons^™

Membrane Topology Of The Colicin A Pore-Forming Domain Analyzed By Disulfide Bond Engineering, Denis Duche, Jacques Izard, Juan M. Gonzalez-Manas, Michael W. Parker, Marcel Crest, Daniel Baty

Department of Food Science and Technology: Faculty Publications

Four colicin A double-cysteine mutants possessing a disulfide bond in their pore-forming domain were constructed to study the translocation and the pore formation of colicin A. The disulfide bonds connected a-helices 1 and 2, 2 and 10, 3 and 9, or 3 and 10 of the poreforming domain. The disulfide bonds did not prevent the colicin A translocation through the Escherichia coli envelope. However, the mutated colicins were able to exert their in vivo channel activity only after reduction of their disulfide bonds. In vitro studies with brominated phospholipid vesicles and planar lipid bilayers revealed that the disulfide bond that …

Fire Frequency And The Vegetative Mosaic Of A Spruce-Fir Forest In Northern Utah, Linda Wadleigh, Michael J. Jenkins

United States Department of Agriculture, Forest Service / University of Nebraska-Lincoln: Faculty Publications

Fire scar and vegetative analysis were used to construct a fire history for the Engelmann spruce/subalpine fir (Picea engelmannii/Abies lasiocarpa) vegetation type of the Utah State University (USU) T. W Daniel Experimental Forest. Three distinct periods of fire frequency were established-presettlement (1700-1855), settlement (1856-1909), and suppression (1910-1990). Mean fire interval (MFI) decreased during the settlement period and greatly increased during the suppression era. The difference was attributed to the influx of ignition sources during the settlement of nearby Cache Valley, located 40 km to the west. Logging and livestock grazing appear to have led to the reduced MFI, …

Identification And Characterization Of A Rapd-Pcr Marker For Distinguishing Asian And North American Gypsy Moths, K.J. Garner

United States Department of Agriculture, Forest Service / University of Nebraska-Lincoln: Faculty Publications

The recent introduction of the Asian gypsy moth (Lymantria dispar L.) into North America has necessitated the development of genetic markers to distinguish Asian moths from the established North American population, which originated in Europe. We used RAPD-PCR to identify a DNA length polymorphism that is diagnostic for the two moth strains. The polymorphism maps to an autosomal locus with codominant Mendelian inheritance. DNA sequence analyses of the Asian and North American forms enabled development of locus-specific primers so that this marker, designated FS-1, will be useful for strain identification under varying conditions in different laboratories.

A Mammalian 2-5a System Functions As An Antiviral Pathway In Transgenic Plants, Amitava Mitra, Dan W. Higgins, William G. Langenberg, Huiqin Nie, Dibyendu N. Sengupta, Robert H. Silverman

Department of Plant Pathology: Faculty Publications

Resistance to virus infections in higher vertebrates is mediated in part through catalysis of RNA decay by the interferon-regulated 2-5A system. A functional 2-5A system requires two enzymes, a 2-5A synthetase that produces 5"phosphorylated, 2',5'-linked oligoadenylates (2-5A) in response to double-stranded RNA, and the 2-5A-dependent RNase L. We have coexpressed these human enzymes in transgenic tobacco plants by using a single plasmid containing the cDNAs for both human RNase L and a low molecular weight form of human 2-5A synthetase under control of different, constitutive promoters. Expression of the human cDNAs in the transgenic plants was demonstrated from Northern blots, …

Molecular Diagnostics Of Three Diabrotica (Coleoptera: Chrysomelidae) Pest Species, Allen L. Szalanski, Thomas O. Powers

Department of Plant Pathology: Faculty Publications

A 257 bp region of the mitochondrial ND4 gene was analyzed for genetic variation in three species of corn rootworm, southern corn rootworm (Diabrotica undecim punctata howardi Barber, SCR), northern corn rootworm (D. barberi Smith and Lawrence, NCR), and western corn rootworm (D. virgifera virgifera LeConte, WCR). Nucleotide sequencing revealed 26 polymorphic sites. Genetic distances averaged 8% for all pair-wise comparisons among the three species. Restriction maps were constructed from sequence data and compared to potential species specific restriction sites. Polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) revealed three restriction enzymes (Alu I, Apo I …

Bovine Cell Line Resistant To In Vtro Infection By Bovine Viral Darrheavirus And All Other Known Pestiviruses, Ruben O. Donis, Eduardo F. Flores

School of Veterinary and Biomedical Sciences: Faculty Publications

Bovine cell line resistant to infection by the pestiviruses Bovine Viral Diarrhea Virus (BVDV), Hog Cholera Virus (HCV) and Border Disease Virus (BDV) and all progeny and mutants thereof. A bovine cell line (CRIB) that is resistant to infection by cytopathic and non-cytopathic BVDV and by other Pestiviruses due to a stable, recessive genetic defect which blocks infection at the level of viral entry.

Predicting Cognitive Capacity From Natural History: Examples From Four Species Of Corvids, Russell P. Balda, Alan Kamil, Peter A. Bednekoff

Papers in Behavior in Biological Sciences

Birds have been studied for centuries because they are numerous, conspicuous, and aesthetically pleasing to humans. Despite their overall regard for birds, historically, many ornithologists have considered birds as instinct-driven organisms of little intellectual capacity. For example, the ornithological textbook of choice from the 1960s states the following view of avian intelligence:

Flight has proven to be an enormously successful evolutionary venture, but one that has cost birds dearly in mental development. In effect, problems merely by flying away from them. … As a consequence, much [avian] behavior is, by mammalian standards, fragmentary, stereotyped, and at times amazingly stupid. (Welty, …

Review Of Linda W. Davis, Weed Seeds Of The Great Plains, Svata M. Louda

Svata M. Louda Publications

The purpose of this useful 145-page book is to provide both a source of comparative information on seeds of 280 weedy plant species in the Great Plains and a means of identifying them. ... The 286 color photographs are the most valuable part of the book. ... Overall, I am delighted to have this book and recommend it to anyone who needs to identify weed seeds and, in fact, to anyone fascinated by the beauty and variation among plants displayed in nature.

Faunal Diversity Among Avian Parasite Assemblages: The Interaction Of History, Ecology, And Biogeography In Marine Systems, Eric P. Hoberg

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Systematics and parasite biodiversity provide power and predictability in broad studies of history, ecology and biogeography in marine systems. Parasitic helminths are elegant markers of contemporary and historical ecological relationships, geographic distribution and host-phylogeny. Complex life cycles of helminths are strongly correlated with intricate food-webs. Dependence on a series of intermediate, paratenic, and definitive hosts indicates that each parasite species represents an array of organisms within a community and tracks broadly and predictably across many trophic levels. Host and geographic ranges of parasites are historically constrained by genealogical and ecological associations, and these determinents interact resulting in characteristic parasite community …

A Sandwich Enzyme-Linked Immunosorbent Assay (Elisa) For The Quantitation Of Peanut In Foods, S. L. Taylor, Julie A. Nordlee, S. L. Hefle

Department of Food Science and Technology: Faculty Publications

Peanuts are one of the most allergenic foods known. Strict avoidance of peanut by peanut-allergic individuals is difficult and often unsuccessful. Peanut proteins have previously been found in nonpeanut foodstuffs prepared on shared processing equipment, and such carryover contamination increases the risk of occurrence of allergic reactions. Immunoassays offer a specific, sensitive, and rapid technique to detect and quantitate small amounts of proteins in food systems.

A sandwich-type, enzyme-linked immunosorbent assay (ELISA) was developed for the detection of peanut protein in foods. Selected food samples were ground and extracts prepared by overnight extraction in 0.01 M phosphate buffered saline, followed …

Allergenic Foods, Susan L. Hefle, Julie A. Nordlee, Steve L. Taylor

Department of Food Science and Technology: Faculty Publications

Virtually all food allergens are proteins, although only a small percentage of the many proteins in foods are allergens. Any food that contains protein has the potential to cause allergic reactions in some individuals. However, a few foods or food groups are known to cause allergies on a more frequent basis than other foods. At a 1995 consultation on food allergies sponsored by the Food and Agriculture Organization, a group of international experts confirmed that peanuts, soybeans, crustacea, fish, cow’s milk, eggs, tree nuts, and wheat are the most common allergenic foods. These foods are responsible for more than 90% …

Analytical Perspectives On A Protohistoric Cache Of Ceramic Jars From The Lower Colorado Desert, James M. Bayman, Richard H. Hevly, Boma Johnson, Karl J. Reinhard, Richard Ryan

Karl Reinhard Publications

A cache of hermetically sealed ceramic jars found in the Lower Colorado Desert was examined using chronometric dating, pollen and macrofossil extraction, design analysis, and water retention experimentation. The cache apparently dates to the protohistoricfifteenth through seventeenth centuries. Findings from these studies contribute to knowledge in four problem areas: (1) ceramic jar function and use-history; (2) storage technology and caching behavior; (3) ceramic dating and chronology; and (4) symbolic iconography. Biotic remains from inside the jars document their use for transporting a variety of riverine and desert plants, before they were finally filled with flowers and seeds, and placed in …

The Synlophe And Other Structural Characteristics Of Sarwaria Bubalis (Nematoda: Trichostrongyloidea) From Cattle In Guyana, J. Ralph Lichtenfels, Eric P. Hoberg, Patricia A. Pilitt, T. M. Craig

Harold W. Manter Laboratory of Parasitology: Faculty Publications

The synlophe (longitudinal, surface cuticular ridges) of Sarwaria bubalis is described for the first time. It is a tapering lateral synlophe of about 40 ridges. The synlophe of S. bubalis is similar to that of Ostertagia ostertagi but markedly different from that of species of Spiculopteragia and Mazamastrongylus. New information is provided also on the structure of the esophagus and perivulval pores. The esophageal valve is more than twice as long as wide. The bilateral perivulval pores were located 192-267 μm posterior to the vulva and dorsal to the lateral lines. The new information will be useful in a …

Carbonate And Gypsum, Richard H. Loeppert, Donald L. Suarez

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The carbonate minerals and gypsum exert a dominating influence on soils in
which they are present because of their relatively high solubility, and in the case
of the carbonates, their alkalinity and pH buffering properties. In this chapter,
procedures for the determination of quantity, reactivity and equilibrium relations
of these minerals will be discussed.

Leafy Spurge (Euphorbia Esula) Genotype Affects Gall Midge (Spurgia Esulae) Establishment, Rodney G. Lym, Scott Nissen, Martha Rowe, Donald J. Lee, Robert A. Masters

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Greenhouse cage studies were conducted to determine the influence of shoot morphology and genetic variation on establishment of Spurgia sulae gall midge on seven leafy spurge genotypes. The genotypes were collected from South Dakota, North Dakota, Nebraska, Montana, Wyoming, Manitoba, and Austria. Genotypes from South Dakota and Nebraska were most susceptible to gall formation and had the highest larvae survival, while the genotypes from Montana and Manitoba were most resistant. Morphological characteristics of the leafy spurge stem tips, such as stem diameter leaf, length, width, and area did not correlate with gall formation or larvae survival. Chloroplast DNA restriction fragment …

Leptodactylus Silvanimbus (Amphibia: Anura: Leptodactylidae): Natural History Notes, Advertisement Call, And Relationships, W. Ronald Heyer, Rafael O. De Sá, James R. Mccranie, Larry David Wilson

Biology Faculty Publications

In July 1995, we visited two of the three previously known localities for Leprodactylus silvanimbus in Departamento de Ocotepeque, Honduras. We found a new breeding site for the species, recorded its advertisement call, and collected and observed its adults and larvae. The purpose of this paper is to present the results of these new findings and to comment on the relationships L. silvanimbus.

Atlas Of Western Australian Agricultural Statistics 1993-94, Dennis Van Gool, Werner Runge

Agriculture reports

Maps by local government areas of agricultural statistics for the 1993-94 financial year to assist strategic planning

Soil Information Sheets For Part Of The Jerramungup Agricultural Area, Tim D. Overheu, Landcare Western Australia, Jerramungup Land Conservation District Committee

Agriculture reports

Each sheet summarises information on the soil's characteristic properties, and associated land use suitability and management. A photograph of a representative profile is provided for each soil. Technical terms are defined in soil sheet glossary. Information is presented under the following headings: soil series and groups, occurence, native vegetation, soil profile description, characteristic soil properties, soil classification and agricultural land use and management.

Pilbara Steer Growth Evaluation : 1994 - 1996, Wayne Fletcher, Brian Mcintyre, Shane Cridland Dr, J L. James

Agriculture reports

Growth potential of steers in the Pilbara - a summary. The trial was conducted over a range of conditions on three locations. The pasture type at Wyloo, the Ashburton River frontage, which is regarded as one of the most productive pasture types in the area, combined with conservative stocking, a fresh paddock and excellent seasonal conditions during 1995, gives us an indication of the District's potential. In extrapolating any of these data to other cases, consideration must be given to adjustment base

Red Blood Cell Stability In Uremic Rats, Cecilia Mermel Maccallum

Theses and Dissertations

Determining the fragility of the red blood cell (RBC) is important for the diagnosis of and evaluation for treatment of several RBC diseases. In part RBC production is controlled through the hormone erythropoietin secreted by the kidney. In a previous study from this laboratory, it was found that RBC were more stable in uremic male rats compared to controls. In this experiment, uremia was induced in four groups of female rats through a two stage nephrectomy. The nephrectomy involved the removal of two-thirds of the left kidney, followed by the removal of the entire right kidney one week later. The …

Growth Of Bluegill, Largemouth Bass, And Channel Catfish In Relation To Fish Abundances, Food Availability, And Other Limnological Variables, Sean P. Callahan

Masters Theses

Bluegill, largemouth bass, and channel catfish are widely distributed and popular sports species. We assessed growth of sympatric populations of these species in 14 reservoirs throughout Illinois, and attempted to relate growth to environmental factors. Principal components analysis (PCA) classified the lakes using 20 morphometric and limnological variables. We examined growth rate relationships with the principal components, via correlations, and also developed simple and multiple regression models using individual variables. Using size-specific growth for two size classes, percent littoral zone of a lake was correlated with growth of both channel catfish (300 and 450 mm) and small bluegill (50 mm). …

Physiological Responses Among Power Air Ball, Spri Tube, And Arm Movement During Step Aerobics, Lisa Dallas

Masters Theses

This study compared caloric expenditure between three step aerobic dance routines (STEP) using the same arm movements; one without any upper body resistive exercise product (ARM), one with a 2 lb. Power Air Ball (BALL), and one with a Spri Tube (TUBE). Twenty-seven college aged females familiar with step aerobics volunteered to be subjects. Maximal oxygen uptake was measured using the Bruce protocol. A 55 minute video was followed for each of the STEP routines to control for arm and leg movement. The order of the STEP routines was randomly assigned. An Aerosport TEEM 100 gas analyzer calculated and recorded …

Mechanistic Studies On Malate Dehydrogenase From Escherichia Coli, S. Kirk Wright

Masters Theses

Kinetic studies and chemical modification studies were performed on malate dehydrogenase from Escherichia coli (eMDH). Chemical modification studies using diethylpyrocarbonate and iodoacetic acid, along with log V/K profiles indicate that one ionizing group with a pK_a between 7.9 and 8.5 acts as a general acid/base in the catalytic mechanism. Log V profiles suggest that malate binds to the correctly protonated form of the enzyme. These results imply that a histidine residue is required for catalysis.

A primary deuterium kinetic isotope effect of 1.43 (±0.14) was observed on V/K using malate-2-d, while no isotope effect was measured on V_{max …}

The Influence Of Environmental Ph And Bicarbonate Concentration On The Excretion Of Nitrogenous Waste Products In Two Species Of Freshwater Teleost, Paul D. Fabsits

Masters Theses

The catabolism of amino acids derived from dietary protein results in the production of ammonia and bicarbonate ion, waste products that an animal must eliminate. Often, animals excrete a nitrogenous waste product that is correlated with water availability: Freshwater fish, living where water is abundant, usually excrete ammonia while terrestrial animals excrete urea or uric acid. Recent studies report that fish inhabiting alkaline waters (pH 10) characterized by high bicarbonate concentrations do not excrete ammonia, instead synthesize urea, an energetically expensive process. The conversion to urea excretion by a normally ammonotelic animal is likely to be stimulated by a specific …

Nutritional And Alimentative State Of The Canaviri, Pocohata, Colina Blanca, Puente Arriba And Villa Arriendo, Ingavi Province, Department Of La Paz - 1996, Juana F. Moraima Gonzles Tapia

Theses and Dissertations

The intent of the present work responds to the necessity of the settlers in depressed areas of La Paz's Altiplano in Bolivia of knowing the reality surrounding the unfolding of their lives. The focus takes into account only one part of all that weighs down on them: that part dealing with their nutritional and alimentative state. This study takes in the communities of Canaviri, Pocohata, Colina Blanca, Puente Arriba, and Villa Arriendo of the Ingavi Province in the Department of La Paz. The variable taken into account is the families' nutritional situation; these families possessing the same status in the …

Physical Condition And Reproductive Success Of White-Tailed Deer At Fort Chaffee, Ar: A Five-Year Study, Donald A. Phillips

Masters Theses

A baseline study conducted in 1991 demonstrated that the resident white-tailed deer (Odocoileus virginianus) population at Fort Chaffee, Arkansas was in poor condition, with below average reproductive success, body weight, and antler size, and above average parasite load compared to other regional deer populations. Consequently, a management program was initiated in 1991 which included: (1) neutralizing acidic soils, (2) prescribed burning, (3) planting supplemental food plots, and (4) liberalizing the harvest of female adults from the population. The purpose of this study was to monitor changes in the health and reproductive success of the herd as this program was implemented. …

Parental Investment In The Red-Eared Slider Turtle, Trachemys Scripta, Michael D. Marlen

Masters Theses

This study was conducted to determine egg and hatchling components of the red-eared slider (Trachemys scripta). In addition, energy components transferred from egg to hatchling were used to determine the level of parental investment in embryogenesis and hatchling care. Eggs of the red-eared slider, collected from central Illinois ponds, were obtained by inducing gravid females to lay by an injection of oxytocin. Egg and hatchling lipids were extracted with petroleum ether while egg and hatchling protein content was determined using the micro-Kjeldahl procedure. Eggs averaged 70.7% water by mass, and dry mass of whole eggs and egg yolks averaged 2.4g …

Small Mammal Community Structure In Restored Prairies, Kathleen A. Falout

Masters Theses

The Illinois Department of Natural Resources currently manages approximately 4,000 hectares of natural prairie. High human impacts coupled with small patch size result in challenging management decisions for prairie conservation. The ideal restored prairie would consist of a mosaic of habitats varying in vegetation from forb-grass dominated areas to oak savannas, with concurrent stages of vegetative succession occurring across the habitats. A study was conducted at the Middle Fork State Fish and Wildlife Area located in Vermillion County, IL, to analyze small mammal diversity and community composition among three different stages of prairie restoration.

The three treatment categories were: fallow …

Physical Linkage Of The Vibrio Cholerae Mannose-Sensitive Hemagglutinin Secretory And Structural Subunit Gene Loci: Identification Of The Mshg Coding Sequence., Jane W. Marsh, Daxi Sun, Ronald K. Taylor

Dartmouth Scholarship

Vibrio cholerae O1 expresses a variety of cell surface factors which mediate bacterial adherence and colonization at the intestinal epithelium. The mannose-sensitive hemagglutinin (MSHA), a type IV pilus, is a potential attachment factor of the V. cholerae El Tor biotype. We describe a TnphoA mutant that is defective in its ability to hemagglutinate mouse erythrocytes. The TnphoA insertion maps to a recently identified genetic locus that encodes products that are predicted to be essential for assembly and export of the MSHA pilus. Insertional disruption at this locus in a mshA-phoA reporter strain provides evidence for a role of this locus …

Annual Review Of The World Pheasant Association 1995/1996, World Pheasant Association

Galliformes Specialist Group and Affiliated Societies: Reports and Other Materials

Pheasant Specialist Group, Peter J. Garson

Partridge, Quail and Francolin Specialist Group, Simon D. Dowell

Grouse Specialist Group, Matt Ridley

The Seventh International Grouse Symposium, Fort Collins, USA, Anne Westerberg

Megapode Specialist Group, René W. R. J. Dekker

Captive Breeding Advisory Committee, Han Assink

WPA-South Asia Regional Office and captive crested argus, Rahul Kaul

Brown eared-pheasant project report. Zhang Zheng-wang

Saving the Blyth's tragopan. Ron Sumner

Why are Lophura pheasants so variable? Geoffrey Davison

Recent rare pheasant observations in Chin State, West Myanmar (Burma), Ben King, Dave Farrow, Craig Robson, Hugh Buck. Tim Fishe