Life Sciences Commons^™

Determination Of Colchicine In Gloriosa Superba L. By Enzyme-Linked Immunosorbent Assay(การวิเคราะห์หาปริมาณ, Colchicine ในดองดึง(Gloriosa Superba L.) โดยวิธี Enzyme - Linked Immunosor..., Vimolmas Lipipun, Wanchai De-Eknamkul, Malai Luempol

The Thai Journal of Pharmaceutical Sciences

An enzyme-linked immunosorbent assay (ELISA) was developed for the determination of colchicine, the active constituent in Gloriosa superba. The measuring range of the assay was from 10 to 105 ng/ml of colchicine. The developed ELISA was highly sensitive ...

Preparation Of Indomethacin Solid Dispersion Tablets Using Lactose And Polyvinylpyrrolidone As Single And Combined Water-Soluble Carriers(การเตรียมยาเม็ดอินโดเมธาชินโซลิดดิสเพอ..., Pienkit Dangprasirt, Sansanee Pongwai, Prawalwan Sitthitrai, Withaya Aloondacharchai

The Thai Journal of Pharmaceutical Sciences

Indomethacin solid dispersions were prepared by coprécipitation method using lactose and polyvinylpyrrolidone (PVP K30) as single and combined water-soluble carriers. The prepared coprecipitates were incorporated into tablets by direct compression proce...

Development Of Skin Stripping Technique To Evaluate In Vivo Percutaneous Absorption Of Diclofenac. I. Effects Of Skin Stripping Sequence, Occlusion Time, And Sites Of Application, Parkpoom Tengamnuay, Wanthanee Samitamarn, Uthai Suvanakoot

The Thai Journal of Pharmaceutical Sciences

Several methods are available for the evaluation of percutaneous absorption of topical drug formulations. The most commonly used in vivo methods are the determination of drug level in plasma and measurements of pharmacological and/or therapeutic response...

Phase Ia/Ib Trial Of Anti-Gd2 Chimeric Monoclonal Antibody 14.18 (Ch L4.18) And Recombinant Human Granulocyte-Macrophage Colony-Stimulating Factor (Rhgm-Csf) In Metastatic Melanoma, James Murray, Eugenie Kleinerman, Shu-Fang Jia, Michael Rosenblum, Omar Eton, Antonio Buzaid, Sewa Legha, Merrick Ross, Lora Thompson, Mansoor Saleh

Haematology and Oncology, East Africa

We performed a phase Ia/Ib trial of chimeric anti-GD2 monoclonal antibody 14.18 (ch14.18) in combination with recombinant human granulocyte-macrophage colony-stimulating factor (rhGM-CSF) to determine the maximum tolerated dose as well as immunologic and biologic responses to the regimen. Sixteen patients with metastatic malignant melanoma received escalating doses of ch14.18 (15-60 mg/m2) administered intravenously for 4 h on day 1. Twenty-four hours later, subcutaneous injections of rhGM-CSF were administered daily for a total of 14 days. Significant side effects were related to ch14.18 infusion and consisted of moderate to severe abdominal and/or extremity pain, blood pressure changes, headache, nausea, diarrhea, peripheral …

Cytogenetic And Isozyme Analysis Of Two Species Of North American Cyprinid Fishes : Rhinichthys Cataractae And Nocomis Micropogon From The Cheat River Drainages Of West Virginia, Syam B. Stoll

Theses, Dissertations and Capstones

Nocomos micropoqon and Rhinichthys cataractae are species of cyprinid fishes that may hybridize to produce a nominally described species Rhynichthys bowersi. Rhynichthys bowersi is a rare fish endemic to a few watersheds mostly in West Virginia, especially the Cheat River drainage. The resolution of its proper taxonomic status is presently a concern to state and federal regulatory agencies. A number of investigators have determined relatedness of cyprinid fishes by examining chromosomal structure. Telomeres and nucleolar organizer regions are chromosome structures used in inter and intraspecific phylogeny. In this study, fibroblasts from N. micropoqon and R. cataractae were first …

Mutations In Trans-Acting Factors Affecting Mrna Decapping In Saccharomyces Cereviciae, Lianna K. Etchberger, C. A. Stevens, A. Stevens, R. Parker

Biology Faculty Publications

The decay of several yeast mRNAs occurs by a mechanism in which deadenylation precedes decapping and subsequent 5'-to-3' exonucleolytic decay. In order to identify gene products required for this process of mRNA turnover, we screened a library of temperature-sensitive strains for mutants with altered mRNA degradation. We identified seven mutations in four genes that inhibited mRNA turnover. Two mutations were alleles of the XRN1 5'-to-3' exoribonuclease known to degrade mRNAs following decapping. One mutation defined a new gene, termed DCP1, which in subsequent work was demonstrated to encode a decapping enzyme or a necessary component of a decapping complex. The …

Fate Of Biological Control Introductions: Monitoring An Australian Fungal Pathogen Of Grasshoppers In North America, Michael J. Bidochka, Scott R. A. Walsh, Mark E. Ramos, Raymond J. St. Leger, Julie Cl Silver, Donald W. Roberts

Biology Faculty Publications

In North America there are two generally recognized pathotypes (pathotypes 1 and 2) of the fungus Entomophaga grylli which show host-preferential infection of grasshopper subfamilies. Pathotype 3, discovered in Austra- lia,hasabroadergrasshopperhostrangeandwasconsidered to be a good biocontrol agent. Between 1989 and 1991 patho- type3wasintroducedattwofieldsitesinNorthDakota.Since resting spores are morphologically indistinguishable among pathotypes, we used pathotype-specific DNA probes to con- firm pathotype identification in E. grylli-infected grasshop- pers collected at the release sites in 1992, 1993, and 1994. In 1992, up to 23% of E. grylli-infected grasshoppers of the subfamilies Melanoplinae, Oedipodinae, and Gomphocerinae were infected by pathotype 3,with no infections >1 km …

Use Of Stems Of Emergent Plants For Oviposition By The Florida Applesnail, Pomacea Paludosa, And The Implications For Marsh Management, Richard L. Turner

Ocean Engineering and Marine Sciences Faculty Publications

This field study examined the distribution among emergent plant stems of egg clutches deposited by the amphibious Florida applesnail in four types of marsh in east central Florida. Clutch density was highest along the edge of sawgrass marsh and zero in deep marsh. Densities in maidencane and mixed shallow marshes were about one-third the density in sawgrass. In all marsh types, clutches occurred preferentially on plants having broad stems that generally exceeded 6 mm in diameter at water level. Substratum limitation for oviposition increased with the frequency of narrow-stemmed plants, but stem height was rarely limiting. The four marsh types …

V, D And J Gene Rearrangements On The Unexpressed Igh Allele In Rabbit B Cells, Chainarong Tunyaplin

Dissertations

No abstract provided.

Characterization Of Central Serotonergic Alterations In Male Rats Following Prenatal Exposure To Fluoxetine, Theresa M. Cabrera

Dissertations

No abstract provided.

Nonsense Codon-Mediated Degradation Of Immunoglobulin Heavy-Chain [Mu] Messenger Rna In B Cells, Tianhong Li

Dissertations

No abstract provided.

Comparative Pharmacologic Studies On Synthetic And Recombinant Inhibitors Of Thrombin, Demetra D. Callas

Dissertations

No abstract provided.

Regulation Of Phospholipid Biosynthesis In The Yeast Saccharomyces Cerevisiae By The Ume6 Gene, John Charles Jackson

Dissertations

No abstract provided.

Substratum-Associated Microbiota, Christopher G. Peterson, Nancy Tuchman

School of Environmental Sustainability: Faculty Publications and Other Works

No abstract provided.

The Effects Of High Colony Density On Filtration, Mortality And Behavior In The Zebra Mussel, Dreissena Polymorpha, Christopher A. Call

Master's Theses

No abstract provided.

Microglial Reactivity To N-Methyl-D-Aspartate (Nmda) Induced Excitotoxicity In The Immature Rat Brain, Laia Acarin

Master's Theses

No abstract provided.

Intraspecific Life History Variation In Sceloporus Undulatus: A Factor Analysis, Geoffrey R. Smith, John W. Rowe, R. E. Ballinger

Faculty Publications

No abstract provided.

The Effects Of Thyroid Hormone On Myosin Heavy Chain A-7 Production In Xenopus Laevis Tail And Leg Muscles, Andrew R. Noble

Honors Theses

The present research examines the expression of myosin heavy chain genes in muscle fibers in Xenopus laevis. Characteristically different muscle fibers are expressed before and after metamorphosis. The embryonic myosin proteins that are synthesized during the tadpole stages are replaced with adult myosin heavy chains at metamorphosis. At least one of the adult myosin genes, called A-7, is expressed only in the adult animals, not in tadpoles. The controlling factor or trigger for the expression of this adult gene still remains unknown. There are a number of possible explanations for the A-7 regulation at metamorphosis, including changes in particular hormone …

Modeling Diseased Oyster Populations. Ii. Triggering Mechanisms For Perkinsus Marinus Epizootics, Eric N. Powell, John M. Klinck, Eileen E. Hofmann

CCPO Publications

Densities of Crassostrea virginica remain high enough to support substantial fisheries throughout the Gulf of Mexico despite high mortality rates produced by the endoparasite Perkinsus marinus. The infrequency of epizootics in these populations suggests that controls exist on the disease intensification process. The progression of epizootics in oyster populations, the factors that trigger epizootics, and the factors that terminate epizootics once started were investigated with a coupled oyster population—P. marinus model.

The time development of a simulated epizootic was triggered by environmental conditions that occurred and disappeared as much as t8 months prior to the onset of mortality in …

Effects Of Shifting To Crowded Or Solitary Conditions On Pheromone Release And Morphometrics Of The Desert Locust, Schistocerca Gregaria (Forskal) (Orthoptera: Acrididae), Arop Leek Deng, Baldwyn Torto, Ahmed Hassanali, E. E. Ali

All Peer-Reviewed Publications

We have studied the effect of shifting the desert locust, Schistocerca gregaria (Forskal) from crowded to solitary conditions, or vice versa on the emission of the adult aggregation pheromone (as measured by released phenylacetonitrile) and compared this with changes in morphometrics. Adult males of the F0 generation resulting from shifting crowd-reared (gregarious) hoppers, fledglings or mature adults (aged 20-22 days after the final moult) to solitary conditions did not produce phenylacetonitrile, similar to solitary-reared adults. Conversely, adults of the F0 generation resulting from shifting solitary-reared (solitarious) hoppers, fledglings or mature adults to crowded conditions produced pheromone at levels which were …

Effect Of Light/Dark Transition On Carbon Translocation In Eelgrass Zostera Marina Seedlings, Richard C. Zimmerman, Randall S. Alberte

OES Faculty Publications

Carbon translocation in the marine macrophyte Zostera marina L. (eelgrass) was investigated to elucidate the impact of light/dark transitions on sucrose partitioning between roots and shoots. After exposure of leaves to C-14-bicarbonate, the level of C-14-labelled photoassimilates increased monotonically in both leaves and fully aerobic roots of plants maintained in the light. Accumulation of C-14 in roots and leaves ceased abruptly when plants were transferred to darkness that induced root anaerobiosis even though C-14 levels remained high in the dark-exposed leaves. Thus, translocation of C-14 photoassimilates from shoots to roots was inhibited when roots became anoxic. Anoxia induced by light …

Development And Lipid Composition Of The Harpacticoid Copepod Nitocra Spinipes Reared On Different Diets, Gregory M. Weiss, George B. Mcmanus, H. Rodger Harvey

OES Faculty Publications

We reared the harpacticoid copepod Nitocra spinipes on diets of bacteria, a diatom, or a macroalga, evaluating survivorship and growth in short-term (≤ 1 generation) experiments. Lipid content of the copepods and their diets was measured and used as an index of nutrition. Although growth, survivorship and lipid content of N. spinipes were significantly greater when fed the diatom, which had the highest lipid content of the 3 diets, the copepod was able to develop from egg to adult when fed a lipid- poor bacterial diet. Furthermore, this species was able to go through developmental molts without the addition of …

Reproductive Ecology Of Pinckneya Bracteata (Bartram) Raflnesque (Rubiaceae), George H. Rountree

College of Graduate Studies: Theses & Dissertations (1964–2006)

Pinckneya bracteata is a small tree or large shrub that is indigenous to the coastal plain of extreme southern South Carolina, Georgia, and northern Florida. It in occurs in sunny, wet habitats and in small populations that are usually separated by distances of kilometers. This study examined the mating, dispersal, and germination systems of the species. Pinckneya bracteata was found to be protandrous and self incompatible. Ruby-throated Hummingbirds and bumblebees ( Bombus spp. appear to be the primary pollinators. Seed fall velocity is important to the wind dispersal of seeds and decreases as seed size increases. Fall velocity is also …

Influence Of Adjacent Seagrass On The Fish Assemblages Off Sandy Beaches, Matthew A. Vanderklift

Theses: Doctorates and Masters

As we attempt to maintain marine biodiversity mainly by focussing on habitats, we need to understand how marine biodiversity is affected by seagrass loss. Although managers and researchers widely acknowledge that habitat loss results in changes to marine biodiversity, quantitative knowledge of these changes is generally poor. In this study, fish assemblages (as one component of the biodiversity of sandy beaches) were examined in Cockburn Sound, Western Australia, to assess patterns that may be related to presence or absence of adjacent seagrass beds, If consistent patterns are evident, they may enable predictions regarding the effects of seagrass loss on the …

Development Of A New Hydroseeding Process For Soil Stabilisation And Vegetation, Eileen T. Keane

Theses

A variety of mechanical and chemical extraction procedures were used to produce crude alginate gels from 3 seaweed species. These viscous hydrocolloidal substances had excellent adhesive properties but did however prove phytotoxic to germinating seeds. Experimental gels were subsequently refined and germination trials were carried out to determine optimum gel types. Pilot field trials were set up to compare the 2 most favourable gels with existing commercial hydroseeding products imported from Norway and Sweden. These tests involved the use of experimental plots on loam and sand substrates with slopes of 0^, 22^ and 45^. A number of biodegradable organic substances …

Use Of Conductance Technology To Determine The Bioactivity Of Oxytetracycline-Hydrochloride, Michael Mc Donagh

Theses

The biological activity of oxytetracycline-hydrochloride was investigated using the indirect conductivity method which is based on the measurement of metabolically generated CO2. A number of laboratory strains and a fresh natural inoculum were exposed to oxytetracycline in the presence and absence of 12% sterile sediment. Results comparing sediment and sediment free systems for all test cultures revealed elevated minimum inhibitory concenrations (MIC) of the agent in the presence of sediment; percentage bioactivity results ranged between 0.50 and 2.07%. pH differences betw'een sediment and sediment-free systems were found not to effect MIC results, and the use of different buffers resulted in …

The Chlorophyll Biosynthetic Enzyme Mg-Protoporphyrin Ix Monomethyl Ester (Oxidative) Cyclase, David Bollivar

Scholarship

No abstract provided.

Introduced Cyprinid (Carp) Fishes In Western Australia And Their Management Implications, K. R. Breheny

Theses : Honours

Australia has no native cyprinid species and five introduced species have established self-sustaining populations. This study examines the cyprinid species present in Western Australia and estimates their distribution. The potential for introduced cyprinids to cause environmental impacts is explained and ameliorative action recommended. The presence and distribution of cyprinid species is assessed by examination of museum records, published literature, reported collections, anecdotal evidence and sampling of wetland habitats. Two species, Carassius nuratus (Goldfish) and Cyprinus carpio (Koi carp) are believed to have established populations and a map of estimated distribution is compiled. The capacity for introduced carp to undergo sudden …

Modelling The Effects Of Rehabilitation And Changed Agricultural Practices In A Saline-Affected Rural Catchment, M. K. Heller

Theses : Honours

The Bremer river catchment, on the South-coast of Western Australia, is typical of most river catchments in this region in that it has been seriously affected by sedimentation, salinisation and eutrophication brought on by the gradual dominance of agricultural land management practices. Vegetated rehabilitation and changed agricultural land management practices (ie minimum / zero tillage) have now been widely adopted throughout the catchment in response to these degradation issues. This study examined the potential impact minimum / zero tillage, vegetated rehabilitation and remnant vegetation could have on both a farm and catchment wide scale. A Geographical Information System was developed …

The Use Of Subjective Ratings Of Perceived Exertion (Rpe) To Estimate Fixed Blood Lactate Concentrations During Incremental Cycle Ergometer Exercise, Keith Robert Scotson

Theses : Honours

Prescription of exercise intensity based on blood lactate concentration has become widely accepted in recent years. The methods used to directly measure blood lactate concentration however, can be costly, time consuming and potentially hazardous to both subject and tester. Recent studies indicate that a strong relationship exists between subjective feelings of strain experienced during exercise and changes in the appearance of blood lactate. This raises the possibility that subjective ratings of perceived exertion (RPE) can be used to simply and effectively estimate and monitor appropriate exercise intensity based on blood lactate concentration. In order to test this theory two groups …