Life Sciences Commons^™

Electrostatic Effects On Airborne Asbestos Monitoring, Roxanne Francis

Biological Sciences Theses & Dissertations

The affects of electrostatic forces on the Membrane Filter Method (MFM) used for air-borne asbestos monitoring have been investigated for many years. Several studies have indicated that these forces interfere with the collection of asbestos fibers on the membrane filter, which results in an underestimated analysis of the airborne concentration. By varying the electroconductivity of the extension cowls on the filter cassettes, it was speculated that the significance of the electrostatic forces could be statistically analyzed. Four sets of filter cassettes with grounded conductive (GC) extension cowls, ungrounded conductive (UC) extension cowls, grounded nonconductive (GN) extension cowls, and ungrounded nonconductive …

Hypercholesterolemia And Platelet Sensitivity To Thromb In-Induced Aggregation In Whole Blood, Charles E. Hammond Ii

Morehead State Theses and Dissertations

A thesis presented to the faculty of the College of Arts and Sciences at Morehead State University in partial fulfillment of the requirements for the Degree of Master of Sciences in Biology by Charles E. Hammond, II on August 1, 1989.

Immunosuppressive Activity Derived From The Uc729-6 Lymphoblastoid And M21-Hpb Melanoma Cell Lines, Charlene J. Repique

Loma Linda University Electronic Theses, Dissertations & Projects

Previous work conducted in our laboratories, using a murine model, suggested that soluble factors secreted by tumor cells suppress lymphocyte responses. To apply this premise to human tumors, we studied the effects of UC729-6 (lymphoblastoid B-cell) and M21-HPB (malignant melanoma) conditioned media (CM) on lymphocyte proliferation assays, as well as on the growth of other cells. The CM was collected at 2-5 day intervals from cultures of UC729-6 and M21- HPB cells in serum-free media. In assays using phytohemagglutinin (PHA) and concanavalin A (ConA), mononuclear peripheral blood cells from healthy human donors showed decreased [³H]-thymidine ([³H]-Tdr) …

In Vitro Evaluation Of Cancer Patient Immune Responses Following Infusion Of Radiolabeled Murine Monoclonal Antibody, Kelly A. King

Loma Linda University Electronic Theses, Dissertations & Projects

Several monoclonal antibody (MAb) types derived from mice have been developed specifically for the use of targeting human tumors. The specificity of these murine MAb’s for their respective tumor antigens is very high making them potentially good immunotherapeutic agents for cancer treatment and diagnosis. A limiting factor in using these murine MAb’s in patients is the development of human anti-mouse antibody (HAMA). The presence of HAMA may reduce the effectiveness of MAb for tumor targeting. If patient sensitization could be quickly detected following the initial infusion of MAb, then future infusion could be altered or terminated to prevent further sensitization. …

Enhancement Of Intratumor Antibody Distribution With A Bifunctional Antibody Delivery System And With Hyperthermia, Karen Lynn King

Loma Linda University Electronic Theses, Dissertations & Projects

The intratumor distribution of a new monoclonal antibody-hapten-based delivery system was examined. From two marine antibodies, Hybritech, Inc. has developed a bifunctional antibody (BPA) with specificities against CEA (ZCE-025 or GEM 231) and against one of two derivatives of [¹¹¹In]-benzyl-EDTA (CHA 255), EOTUBE or BLEDTA IV, to form ZCE/CHA or CEM/CHA. BFA accumulates in CEA-expressing tissue and clears from non-antigenic tissues prior to administration of an [¹¹¹In]-hapten.

Using five groups of nude mice bearing human colon tumor xenografts (T380), we evaluated different carriers of [¹¹¹In]-EOTUBE to prelocalized ZCE/CHA. We administered all antibodies and haptens …

Bluetongue Virus Evolution: Sequence Analyses Of The Gene Coding For The Major Serogroup Antigen, Timothy F. Kowalik

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

A study was undertaken to better understand the genetic relationship of five United States prototype bluetongue virus serotypes. Genomic double-stranded RNA segment S1, which encodes the major core protein and serogroup antigen, VP7, was used as a marker gene for the sequence analyses. The S1 segments from BTV-2, 11, 13, and 17 were cloned and sequenced by methods developed during the course of this investigation. These results were compared with previously published sequence data from segment S1 of BTV-10. The Sl segments are 1156 base pairs long and contain a common open reading frame capable of coding for a protein …

Control Of Viral Mrna Stability By The Virion Host Shutoff Function Of Herpes Simplex Virus, Asha A. Oroskar

Dissertations

No abstract provided.

Genetic Mapping And Characterization Of Acc, The Agrocinopine-Agrocin 84 Region, On Ptic58, The Nopaline-Type Ti Plasmid Of Agrobacterium Tumefaciens Strain C58, G. Thomas Hayman

Dissertations

No abstract provided.

Heterotrophic Bacterial Production: Relationships To Biological And Abiological Factors In Estuarine Environments, Eric Thor Koepfler

Dissertations, Theses, and Masters Projects

Section 1. Ecotoxicological effects of creosote contamination on benthic bacterial communities in the Elizabeth River, Virginia were investigated using both structural and functional microbial parameters. Results indicated that cell specific and total heterotrophic bacterial production parameters were depressed in a dose dependent manner with increasing sediment PAH concentrations. Toxicity effects upon production were modified by temporal trends associated with temperature as well as spatial sediment characteristics. of the parameters employed, the tritiated thymidine production assay was found to be the most sensitive for detection of ecotoxicological effects. Section 2. Bacterial abundance and production were examined during a destratification event in …

Nutritional Influences On Aging In Aspergillus Ornatus, Jaydeep Shantilal Shah

Dissertations, Theses, and Masters Projects

No abstract provided.

Effects Of Selected Nitrogen-Containing Aromatic Compounds (Ncacs) On Physiological Properties In Escherichia Coli, W. James Catallo Iii

Dissertations, Theses, and Masters Projects

This research examined the effects of quinoline and 4-azafluorene on respiratory electron transport rate (ET), outer membrane permeability and topology, oxygen consumption, and viable cell density in Escherichia coli cell suspensions. ET was estimated spectrophotometrically using INT (2-(p-iodophenyl)-3-(p-nitrophenyl)-5-(phenyl)-2H-tetrazolium chloride, which is reduced in vivo to a red colored formazan (INTF). Both test compounds caused anomalous dose-response behavior in INT assays: in a defined window of doses, ET rates near or above the controls were observed. These doses showed altered INT reduction kinetics, decreased cellular oxygen demand, and decreased viable cell densities. Experiments with E. coli spheroplast preparations, gram(+) cells, and …

Characterization Of Steroid-17,20-Desmolase And 20Α-Hydroxysteroid Dehydrogenase From Clostridium Scindens, Amy Elizabeth Krafft

Theses and Dissertations

Clostridium scindens is an obligate anaerobe isolated from the normal intestinal flora of man. It is the only bacterium known at present to synthesize both steroid-17,20-desmolase and 20α-hydroxysteroid dehydrogenase (ZOα-HSDH). This study was undertaken to characterize these neutral steroid transforming reactions in extracts of C. scindens; to purify and characterize the enzymes responsible for side-chain cleavage and 20α- hydroxysteroid oxidoreduction and to clone the genes encoding these enzymes with a view towards studying the mechanism of induction by steroids in a prokaryotic system. Both enzymes were found to be co-inducible in cells cultured in the presence of 17α-hydroxysteroids. In cell …