Life Sciences Commons^™

Unknowing Sacrifices: Public Health And Radiation Illness In New Mexico And Nevada, 1940s-1960s, Beatriz Avila-Marquez

UNLV Theses, Dissertations, Professional Papers, and Capstones

The detonation of the first atomic bomb in the early morning of July 16, 1945, in New Mexico welcomed the atomic age that forever changed the world. After that day, thousands of lives were lost due to nuclear weapons, and hundreds of thousands more continued to suffer from the effects of atomic testing. The Atomic Energy Commission, fueled by the arms race of the Cold War, chose Nevada to continue the United States’ nuclear weapons testing. Knowledge of the dangers of radiation exposure, the effects of radiation, and techniques to prevent exposure are now available in part due to the …

Junctional Actin As A Mechanical Hub For Supracellular Actomyosin Force Transmission During Apical Constriction, Jasneet Brar

UNLV Theses, Dissertations, Professional Papers, and Capstones

Apical constriction is a conserved morphogenetic mechanism that drives epithelial folding during development. During ventral furrow formation in Drosophila melanogaster, contractile actomyosin networks generate forces that reduce apical cell area resulting in tissue invagination. For these cell-scale forces to produce coordinated tissue-scale changes, contractile networks must be mechanically integrated across neighboring cells. Although adherens junctions are known to mediate this coupling, it is unclear how junctions are integrated into cortical actin cytoskeleton, which directly determines cell, and consequently tissue, shape. This thesis tests the hypothesis that supracellular actomyosin organization during apical constriction depends on distinct adherens junction based mechanical couplings: …

Thrombin-Induced Myofibroblast Differentiation Is Regulated By Myocardin And Novel Effector Genes In Ipf Fibroblasts, Christiana Okeke

Biotechnology Theses

Idiopathic pulmonary fibrosis (IPF) is a progressive interstitial lung disease characterized by fibroblast activation, excessive extracellular matrix (ECM) deposition, and irreversible respiratory decline. In this study, we investigated the role of myocardin and its downstream effectors, RGS5, DLGAP5, and TAGLN, in thrombin-induced myofibroblast differentiation and ECM remodeling using primary human IPF fibroblasts. Myocardin knockdown significantly attenuated thrombin-induced expression of profibrotic genes, including FN1, COL1A1, PAI-1, ACTA2, and CNN1, as well as the novel effectors RGS5, DLGAP5, and TAGLN, confirming myocardin as a central regulator of fibrotic signaling. Silencing RGS5 produced minimal effects on gene and protein expression despite minor downward …

Care As Method: Sound, Duration, Relation In Contemporary Art Practice, Violet L. White

MFA in Visual Art

Abstract Care as Method: Sound, Duration, and Multispecies Relation in Contemporary Art Practice

This thesis proposes care as a method of artmaking, not a subject, theme, or aesthetic position, but the actual structural logic through which a body of work is built, sustained, and understood. Working at the intersection of ecological art practice and relational aesthetics, it asks what happens when the encounter at the center of the work is not between two people but between species, infrastructures, and histories of use and what obligations that encounter generates.

The argument is developed through six interconnected works: installations, sound compositions, sculptures, …

Environmental Dna (Edna) Assessment Of Fish And Decapod Diversity And Their Association With Cetacean Distribution In Matang Coastal Waters, Perak, Lim Chuan Hui

Student Works (2020-2029)

The estuarine and coastal regions of Matang, Perak, Peninsular Malaysia, are vital fishing grounds and recognised biodiversity hotspots, but are increasingly exposed to human activities. Understanding the biodiversity of marine fish and decapods is crucial for effective conservation efforts. Conventional biodiversity monitoring methods face several challenges, including invasiveness, inconsistencies in sampling techniques, reduced taxonomic accuracy, time-consuming processes, high costs, and often only providing approximate estimates. In this study, we evaluated the use of environmental DNA (eDNA) metabarcoding to assess marine fish and decapod diversity in Matang waters and to explore possible associations between fish assemblages and local cetacean occurrence. Water …

Understanding Behavioral And Representational Divergences Of Humans And Machines, Thomas Lasman Botch

Dartmouth College Ph.D Dissertations

Human behavior and cognition are strikingly variable: people differ from one another in their preferences and abilities, and even from themselves across situations. Yet this diversity arises from common neural machinery shaped by the complex environments humans inhabit. A central objective of cognitive neuroscience is to understand how this varied experience emerges from interactions between brains, agents, and environments. In this dissertation, I argue that comparing behavior and neural computation across humans, artificial systems, and contexts is essential for understanding the flexibility of human cognition. Across three chapters, I use this comparative approach to examine how the rich, multimodal contexts …

In Vitro Modeling Of Macrophage And Cd8+ T Cell Activation: Evaluating Immortalized Cell Lines For Use In Undergraduate Immunology Laboratories, Lucy Groothuis

Masters Theses

The immune system is a complex, highly coordinated network of cells and signaling molecules that protects the body from infection and disease. Immunology is therefore a fundamental component of undergraduate education for students pursuing careers in medicine and biomedical research. Despite its importance, many undergraduate immunology courses lack a laboratory component. To address this gap, we developed an open-access immunology laboratory manual and evaluated its ability to generate meaningful data using primary murine cells and commercially available cell lines. Given the cost and logistical constraints of using mouse models in an undergraduate laboratory setting, we evaluated whether immortalized cell lines …

Behavioral And Molecular Effects Of 2,4-Dichlorophenoxyacetic Acid Exposure In Planarians, Emily Pintilie

Graduate Student Dissertations, Theses, Capstones, and Portfolios

The widespread use of pesticides, such as 2,4-dichlorophenoxyacetic acid (2,4-D), raises serious concerns about their effects on non-target aquatic organisms. In vertebrates, 2,4-D has been associated with neurotoxic outcomes. Planarians offer a valuable system for toxicological studies because of their well-characterized nervous system, regenerative capacity, and measurable behavioral responses. This study investigated the neurotoxic effects of 2,4-D exposure in the planarian species Girardia dorotocephala, including the assessment of nociceptive behaviors, survival rate, phototactic responses, and DNA methylation. Worms were exposed to various concentrations of 2,4-D (0–100 mg/L) at multiple time points. Nociceptive responses, such as scrunching, C-shapes, and seizures, …

The Detection Of Forever Chemicals From Paper Cups Using Colorimetry, Sara Miller

Graduate Student Dissertations, Theses, Capstones, and Portfolios

Per- and polyfluoroalkyl substances (PFAS) are synthetic chemicals added to food packaging products to increase water-resistance and durability. PFAS are a growing concern due to their migration into beverages and foods and have been linked to cancer, immunotoxicity, and thyroid disturbances. The purpose of this study was to quantify PFAS migration from paper cups into water and evaluate toxic effects using planarians. PFAS detection was performed using a colorimetric assay, which forms a colored complex with PFAS, on: perfluorononanoic acid (PFNA), heptadeca fluorooctane sulfonic acid potassium salt (PFOS), perfluorooctanoic acid (PFOA). PFNA and PFOS showed absorbance at 640 nm, while …

Apoe Ε2 Potential Protection Against Long-Term Consequences Of Traumatic Brain Injury, Rebecca Faith Mesidort

Graduate Student Dissertations, Theses, Capstones, and Portfolios

Traumatic brain injury (TBI) can alter the quality of life, often resulting in cognitive decline, neurodegeneration, or death. There have been no biological advances to resolve the long-term consequences of TBI. The APOE ε2 gene is well known as a neuroprotectant against neurodegenerative diseases such as Alzheimer’s disease and Parkinson’s disease. However, the biological mechanisms of how APOE ε2 protects against neurodegeneration are unknown. The purpose of this study is to investigate APOE ε2’s response to an environmental stimulus that leads to neurodegeneration, TBI, and further investigate APOE ε2’s biological mechanisms. After inflicting blunt force trauma to our model organism, …

Myocardin-Dependent Regulation Of Adam12 And Adam19 Promotes Fibronectin Fibrillogenesis During Pleural Fibrosis, Uday Siva Naga Sankar Allamneni

Biotechnology Theses

Pleural fibrosis involves excessive extracellular matrix (ECM) deposition driven by chronic injury and inflammation. Transforming growth factor-β (TGF-β) induces mesothelial–mesenchymal transition (MesoMT), promoting a myofibroblast phenotype. Our laboratory identified myocardin (Myocd), a transcriptional coactivator, as a key mediator of MesoMT that regulates ECM-remodeling proteases. RNA-seq and qPCR analyses showed that ADAM12 and ADAM19 are upregulated by TGF-β but suppressed by Myocd knockdown in Human pleural mesothelial and IPF-derived cells. Silencing ADAM12 reduced fibrotic markers, including fibronectin and smooth muscle actin (α-SMA), whereas ADAM19 showed relatively minor effects. ADAM12 knockdown also impaired fibronectin fibrillogenesis and insoluble matrix assembly, as confirmed by …

Synthesis Of A Nicotine-Like Backbone Ligand For Binding Evaluation At Plk-1 Pbd, Carter Johnson

Undergraduate University Honors Capstones

Cancer is a significant problem and requires continued research, as it remains one of the leading causes of death worldwide (Ramos, 2021). This capstone explores the synthesis and preliminary evaluation of a nicotine-type compound as a potential cancer-curing agent targeting the PLK-1 PBD protein using fluorescence polarization. The hypothesis is that a specific ligand may contribute to cancer research. This project also reviews cancer management methods while illustrating the discovery of chemical drugs used in chemotherapy. The project involves synthesizing and testing 3-((1-methylpyrrolidin-2-yl)methoxy)-1-alkylpyridin-1-ium, known as Compound C, to determine whether it can function as a potential cancer management agent. The …

The Brain Breakdown: Bringing Neuroscience To A Broader Audience, Katharine Buehrig

Undergraduate Honors Theses

Neuroscience is a rapidly growing field, which needs to continue becoming more accessible and understandable to younger generations. Expanding access to neuroscience through different modes, such as podcasts, brings the discipline to a broader audience. Podcasts allow listeners to follow an outlined learning path they can pursue on their personal timeline. High school students often do not have access to neuroscience specific materials within their standard science curriculum. To address this knowledge gap, I collected questions about neuroscience from high school students across four schools in the United States to develop an understanding about what they are interested in learning. …

Impact Of Land-Use Disturbances On The Composition Of Wetland Plant Communities Across The Conterminous United States, Maya Merchant

Undergraduate Honors Theses

Due to the threat of invasive species on wetland biodiversity, it is critical to assess factors driving their proliferation in wetlands. To address this growing need, this project analyzes relationships between non-native species and known anthropogenic disturbance types associated with increased biological invasions. Using the U.S. EPA’s 2021 National Wetland Condition Assessment, the relationship between non-native species (diversity, richness, and coverage) and surrounding land-use within 500, 1,000, and 3,000-meter buffers was evaluated. To explore patterns across larger spatial scales, mean non-native species diversity, richness, and coverage was calculated at the watershed level and compared to associated percentages of agricultural land …

Effects Of Combined Action Observation, Motor Imagery, And Neuromuscular Electrical Stimulation On Muscle Strength And Size During Short-Term Upper Limb Immobilization, Zahra Pourhatami

Theses - ALL

Short-term limb immobilization induces rapid declines in muscle strength that exceed reductions in muscle size, suggesting a dominant role of neural mechanisms in early functional decline. Interventions capable of simultaneously targeting central neural and peripheral muscular pathways may therefore offer superior protection during disuse, yet such combined approaches remain unexamined in upper limb immobilization models. To investigate the effects of combined action observation with motor imagery (AOMI) and neuromuscular electrical stimulation (NMES) (AOSTIM) on muscle strength, size, and quality during 7 days of upper limb immobilization, we conducted a study in healthy young adults. Forty-three healthy young adults were randomly …

Co-Designing Sweettexts: An Intake-Driven Sms Intervention To Reduce Free-Sugar Consumption In Preschoolers, Bervelyn Asantewaah Addo

Theses - ALL

Abstract Introduction: As early childhood represents a crucial window for establishing lifelong dietary patterns, excessive intake of sugary foods and beverages during this period may result in long-term adverse health outcomes. Intake levels continue to exceed recommended guidelines, and disparities within and across race and low-income population groups persist. Given these disparities, targeted interventions developed to consider the unique needs, preferences, and cultural differences in diverse population groups are essential to enhance health and well-being. The purpose of this research was to develop the content of the "SweetTexts", a text-message-based intervention to reduce the intake of free sugar among preschool-aged …

The Role Of Creatine Supplementation On Neuromuscular Function, Mark Petry

Theses - ALL

This study examined the effects of short-term high-dose creatine monohydrate (CrM) supplementation on neuromuscular and corticospinal function in healthy adults. Creatine is widely recognized for its role in enhancing muscular performance and energy availability, yet its short-term effects on neural and neuromuscular outcomes remain unclear. The purpose of this investigation was to determine whether three days of CrM loading would influence maximal voluntary contraction (MVC) peak torque, corticospinal excitability (CSE) measured via transcranial magnetic stimulation (TMS)-induced motor evoked potential (MEP) amplitude, muscle cross-sectional area (CSA) and muscle quality via echo intensity (EI). Nine healthy creatine-naive adults completed a repeated-measures design …

Identifying And Coping With Failure In Human Anatomy & Physiology I And Ii Students, Annie R. Mueller

Honors Theses

Human Anatomy and Physiology (A&P) is a two-semester course that focuses on the structure and function of the human body systems. For many students, A&P could be their first challenging undergraduate course and their first experience with academic difficulty. Students must learn to adapt their study habits and respond productively to academic failure. The purpose of this study was to examine how students in the A&P courses at the University of Mississippi perceive and cope with academic failure, as well as to evaluate the effectiveness of an intervention method. Students enrolled in BISC 206 and BISC 207 participated in three …

Distribution Of Mussels In Tributary Systems Of The Lower Ouachita River Watershed, Zach R. Crain

ATU Theses and Dissertations 2021 - Present

Freshwater mussel communities in small tributary streams are poorly documented in Arkansas, limiting the understanding of how diversity is structured across river networks. To address this data gap, we surveyed 147 sites across five previously unsurveyed watersheds in the South-Central Plains Ecoregion (SCPE). We sampled 5,473 individuals representing 17 species, including four Arkansas Species of Greatest Conservation Need (SGCN). We evaluated patterns in community variation utilizing β-diversity based on both presence-absence and abundance data. Overall turnover (βsor and Bray) was partitioned into replacement (βsim and BrayBal) and nestedness (βsne and BrayGra) components. Replacement and nestedness each drove turnover in two …

Determining Cave Presence And Diet Variation Of The Ozark Big-Eared Bat (Corynorhinus Townsendii Ingens) From Guano Dna, Lark E. Sybrant

ATU Theses and Dissertations 2021 - Present

The Ozark big-eared bat (Corynorhinus townsendii ingens; OBEB) is federally endangered in Arkansas, where it persists in a few small, geographically restricted populations within the Ozark National Forest. Although the region contains many caves that appear suitable for OBEB, expanded survey efforts are needed to locate additional roost sites for monitoring and protection. Effective conservation also requires a clear understanding of their distribution and diet. Traditional cave surveys can be disruptive and often miss cryptic species, and earlier diet studies based on manually sorting prey remains provided only coarse taxonomic resolution and overlooked soft‑bodied insects. To address these …

Distribution, Habitat, And Fish Assemblages Associated With The Rocky Shiner In Arkansas, Savannah L. Wise

ATU Theses and Dissertations 2021 - Present

The Rocky Shiner (Notropis suttkusi) is a minnow species endemic to the Red River and its tributaries in southern Arkansas and Oklahoma. This species is petitioned for listing under the Endangered Species Act and is a species of greatest conservation need (SGCN) in Arkansas. However, there is little information for the species’ distribution, habitat use, and ecological niche, especially within the Arkansas portion of its range. Because of this data gap, my study provides an updated description of its distribution and habitat associations using conditional occupancy modeling and explores patterns of species co-occurrence within the community. I sampled …

Re-Evaluating The Safety Of Erythritol: A Critical Review Of Emerging Evidence, Kaitlyn B. Kirchner

Honors Thesis

Erythritol, a commonly used non-nutritive sweetener, has gained popularity as a sugar alternative due to its low caloric value and minimal effect on blood glucose levels; however, emerging research has raised questions about its overall safety. This literature review critically evaluates current peer-reviewed evidence on the effects of erythritol on the cardiovascular and gastrointestinal systems. Findings indicate that erythritol is generally well tolerated in the gastrointestinal tract, with fewer adverse effects than other sugar alcohols due to its rapid absorption and limited fermentation, although symptoms may be dose-dependent and influenced by co-ingestion with other carbohydrates. In contrast, evidence related to …

Development Of Water-Soluble Nanofibrous Poly(Vinyl Alcohol) Collectors For Bioaerosol Sampling And Environmental Surface Microbial Detection, Kevin Crown

Theses & Dissertations

Environmental surveillance for pathogenic microorganisms requires sampling technologies capable of efficiently collecting biological particles from air and surfaces for downstream detection.  Current environmental sampling materials, such as fibrous aerosol filters and surface sampling swabs, are designed to capture particles effectively but often retain a portion of the collected material during extraction.  Incomplete recovery reduces the sensitivity of microbiological and molecular detection, particularly in situations where microbiological concentrations are low, such as in environmental monitoring and clearance sampling following biological contamination events.  This dissertation describes the development and evaluation of water-soluble nanofibrous sampling materials designed to improve microbial recovery while maintaining …

Replication And Extension Of Kras-Associated Gene Expression Signatures In Lung Adenocarcinoma: A Cox Proportional Hazards Analysis Of Mutation Status, Gene Expression, Multi-Gene Mutation Count, And Ras De Novo Signature, Camryn Berry

Theses & Dissertations

This study evaluates the robustness and reproducibility of a previously published analysis of lung adenocarcinoma gene expression data (Schabath et al.), which examined KRAS-associated biology in relation to co-occurring STK11 and TP53 mutations. The original study used Kaplan-Meier (KM) survival analysis and gene expression signatures to assess associations between driver mutations, tumor suppressor mutations, and patient outcomes. The aim of this work is to replicate these analyses and evaluate consistency across independent datasets (Wilkerson and Selamat), with extension using Cox proportional hazards (PH) models. Two hypotheses are evaluated: (1) that the number of mutated genes increases the hazard of death, …

Expanding Regenerative Agriculture Practices In East Texas, Treasure Hance

Electronic Theses and Dissertations

Regenerative agriculture has increasingly been referenced since 2015 for its ability to provide healthy food with less environmental and social impacts. Its basic premise/ideology is to follow a process whereby soil, biotic, and human communities are not just maintained but prepared to return to a better state. Soil health is a primary concern. Actions include no-till planting, composting to create more organic soil, less use of synthetic fertilizers and pesticides, along with better employment, animal care, and ecological outcomes/practices. Regenerative versions of other professions, such as regenerative medicine (e.g. gene/stem cell therapy) and regenerative architecture (design for surplus energy, restored …

Role Of Kif4a In The Fxa-Induced Phenotypic Modulation Of Pleural Mesothelial Cells, Sudishna Khanal

Biotechnology Theses

Pleural injuries can disrupt the organization of the pleural surface, leading to pleural remodeling and fibrosis (PF), characterized by the proliferation of myofibroblasts expressing alpha-smooth muscle actin (α-SMA) and the deposition of extracellular matrix proteins, primarily due to the mesenchymal transition of pleural mesothelial cells (PMCs) (MesoMT). It is well established that various external stimuli, such as TGF-β, thrombin, and factor Xa (FXa), induce MesoMT. Our laboratory found that myocardin, a transcriptional co-activator, was dramatically up-regulated during MesoMT, and that gene silencing of myocardin (MyoCD) diminished MesoMT. RNA sequencing data showed that FXa activated a set of genes not upregulated …

The Legacy Of Lead: Lead Exposure's Harmful Effects And Its Concentration In Poc Communities, Layla Sophronia Barber

Student Theses 2015-Present

This thesis examines the disproportionate burden of lead exposure carried by low income, POC communities. The systemic nature of this problem is a symptom of a longstanding legacy of environmental injustice in the United States. Decades of federal neglect are reflected in the higher statistics of lead exposure and poisoning in predominantly black communities. While it is understood that lead exposure poses a serious threat to physical health and early cognitive development, there is a discouraging lack of urgency to remove the toxin from non-wealthy communities. The material covered by this thesis aims to identify and correct the discriminatory social …

Analyzing Sun Bucks Program Experience Among Southwestern Pennsylvania Families With Just Harvest, Olivia Mae Gifford

Public Health Capstone Projects

1. Objectives: This project primarily seeks to identify the common barriers preventing vulnerable families from accessing the SUN Bucks Program. The SUN Bucks program is an optional program through the United States Department of Agriculture that works in conjunction with the state of Pennsylvania in order to provide school-aged children families with supplemental funds to supply greater nutrition during the summer months. This research supports the work of a local non-profit, Just Harvest, in order to better inform their advocacy and outreach efforts as they fight against food insecurity in Western Pennsylvania.
2. Methods: All data was collected electronically via a …

A Sturdy Future: Mangrove Restoration Against Climate Extremes, Gabriella Marie Siasoco

Student Theses 2015-Present

This paper addresses the impacts of climate change on natural disasters and examines how rehabilitation of mangrove forests in Mesoamerica and the Caribbean can mitigate those effects. Rising ocean and atmospheric temperatures contribute to higher sea levels and more severe hurricanes and tropical storms, characterized by increased frequency, duration, and intensity in peak winds, storm surges, and rainfall. Mangrove ecosystems act as vital buffers against coastal disturbances such as tsunamis and extreme storms, while contributing to climate change mitigation through carbon sequestration. Additionally, they improve water quality by filtering nutrients, preventing eutrophication. Despite their ecological and economic value, mangroves in …

Associations Between Levels Of Physical Activity And Cognitive Health, Gemma Goldenbaum

Honors Theses

The purpose of this study is to examine the relationship between physical activity levels and executive function, specifically focusing on inhibitory control and working memory capacity. Though the benefits of exercise on brain health are well-documented, the extent of cognitive differences between sedentary individuals and elite student-athletes remains misunderstood. Eighty participants (ages 18–35) were categorized via the International Physical Activity Questionnaire (IPAQ) into sedentary, low, and advanced activity tiers. Participants completed computational assessments, including the Operation Span (OSPAN) task for working memory and Go/No-Go tasks to measure response inhibition and implicit associations. Preliminary results indicated significant differences in sedentary behavior, …