Life Sciences Commons^™

Providing Proteins To Belizean Residents Through Poultry Products, Ellis Freel

Poultry Science Undergraduate Honors Theses

Belize is a food insecure, developing country. Although Belize has plenty of nutritious food in-country, the exportation of that food to generate income contributed to a distribution issue of that nutritious food. More impoverished areas of Belize do not have access to nutritious food because of the lack of affordability and high export rates. Diets with a lack of food, or lack of balanced, nutritious food can negatively impact growth and cognitive development, especially in children. Poultry is one of the only products in Belize not able to be exported. Implementing poultry into Belizean diets would affordably alleviate food insecurity …

Microbiome Assembly And Function In The Solitary Mason Bee, Osmia Lignaria (Megachilidae), Bailey Crowley

All Graduate Theses and Dissertations, Fall 2023 to Present

Animal-microbe interactions can influence host biology, ecology, and evolution. The assembly and function of microbes found within animal hosts oftentimes depends on which species are involved. Advances in sequencing technologies have permitted the exploration of host-microbe interactions in a variety of animals, including bees. Early research aimed at understanding the microbiomes of social bees, such as honey bees and bumble bees, found that microbes prevent the spoilage of stored pollen, breakdown indigestible nutrients into smaller molecules available for uptake by the host, and also protect the host from pathogens. When environmental stressors, such as increased temperatures, disrupt the microbiome, the …

The Interactions Between Drought Tolerant Corn Hybrids And Plant Water-Stress On Weeds And Their Host Capability For Spider Mites, Mercy Adhiambo Odemba

All Graduate Theses and Dissertations, Fall 2023 to Present

With climate change, it is predicted that more frequent high temperatures and drought severity will lead to an increase in damage caused by pests that thrive under these conditions. Spider mite outbreaks, for example, are associated with plant waterstress and there is evidence that some weeds are more resilient and adapted to drought than some crops, leading to major concerns for the management of these two pest types. Weeds directly compete with crops for limited resources, but what is unclear is which weeds harbor spider mites and whether they exacerbate the impact of spider mites on corn crops under water-stressed …

Positron Emission Tomography In Oncology And Environmental Science, Samantha Delaney

Dissertations, Theses, and Capstone Projects

The last half century has played witness to the onset of molecular imaging for the clinical assessment of physiological targets. While several medical imaging modalities allow for the visualization of the functional and anatomical properties of humans and living systems, few offer accurate quantitation and the ability to detect biochemical processes with low-administered drug mass doses. This limits how physicians and scientists may diagnose and treat medical issues, such as cancer, disease, and foreign agents.

A promising alternative to extant invasive procedures and suboptimal imaging modalities to assess the nature of a biological environment is the use of positron emission …

Flanker Task Performance In Young And Older Adults: A Behavioral And Erp Study, Fatima Medrano

Dissertations, Theses, and Capstone Projects

Research suggests that as we get older, executive function abilities decline (Hasher & Zacks, 1988; Salthouse, 1996). One affected ability is that of inhibitory control, which aids in monitoring our responses to non-target stimuli or information. Current research on inhibition reveals inconsistencies across studies. Monitoring brain responses during the Flanker (used to measure inhibitory control) task may add valuable insight into the processes underlying group differences behaviorally, by studying the N200 and P300 event-related potentials which have been associated with inhibitory control processes. This study investigated whether there are differences between older and younger adults in inhibitory control and whether …

Cross-Linguistic Differences In Neural Encoding And Processing Of Stop Consonants: The Impact Of Language Experience On Attention Allocation, Aline Dos Santos Oliveira

Dissertations, Theses, and Capstone Projects

This study aims to examine the intricate relationship between language experience and the neural processing of stop consonant speech sounds. Previous research has shown minimal differences in amplitudes and latencies of cortical Auditory Evoked Potentials (AEPs) to speech sounds across listeners from various language backgrounds. It is only towards the later latencies (around the P2 peak at 200 ms), that there have been suggestions of cross-linguistic differences. In addition, a recent study, observed an AEP difference between monolinguals and bilinguals when processing speech, specifically an "Nd effect”. The Nd effect, which is an increased negativity of the AEP is proposed …

Systematics And Comparative Genomics Of The Petaluridae (Odonata: Anisoptera), Ethan R. Tolman

Dissertations, Theses, and Capstone Projects

The Anisoptera (Insecta: Odonata), or dragonflies exhibit diverse life histories and diversification patterns, with the family Petaluridae standing out as particularly unique. Comprising only 11 extant species, all of which inhabit increasingly threatened fen habitats, Petaluridae are known for their longevity, which can exceed five years, semi-terrestrial lifestyle, and remarkable physical size, ranking among the largest insects on Earth. Despite the evolutionary peculiarities of this family, no previous genomic studies have investigated the Petaluridae. This work presents two high-quality genome sequences and a targeted enrichment dataset to explore the chromosome architecture of Petaluridae, identifies genomic adaptations associated with their distinctive …

Integrating In Vitro And In Silico Approaches To Gain Insight Into The Mechanism Of Amyloid Fibrillogenesis, Marvin M. Bilog

Dissertations, Theses, and Capstone Projects

Amyloid fibril formation, the hallmark of numerous amyloid-related diseases, has been the subject of a vast number of scientific studies due to its pathological implications. Since the fibrillization process exhibits a certain level of intricacy, its investigation requires a multidisciplinary approach that integrates both experimental and computational methods. In vitro techniques involve biophysical assays and imaging tools for characterizing the structural and kinetic aspects of amyloid fibril formation. In parallel, in silico techniques offer programs for predicting atomistic details and behaviors of amyloidogenic proteins and peptides at the nanoscale level. Serum amyloid A (SAA), human islet amyloid polypeptide (hIAPP), and …

Naturify 2300, Yarina Yiwei Dai

Masters Theses

In my art practice, I explore the interplay between human desires to manipulate and anthropomorphize nature, as seen in the technological augmentation of plants and living entities. This investigation delves into how this intersection, alongside empathy towards these creations, contributes to fears of uncontrollability and the risks of addiction and excessive dependence on technology.

Bioengineering and genetic modification have cultivated unprecedented developments, allowing humans to manipulate the fundamental building blocks of life. My research speculates on this technology further, modifying the genetic code of organisms and creating bioengineered wearable entities with enhanced traits or entirely new functionalities. The primary objective …

Sircling The Cquare, Latika Balachander

Masters Theses

As we journey into an increasingly virtual and intangible reality, is there an opportunity for our tactile fabrics to journey with, and even reorient us? Can they exist in our future worlds to remind us of the value of current traditional, low-tech practices that we may soon forget?

Referencing the fundamental fabric languages of knitted and woven structures, this collection of garments, that I term “Earthsuits,” embody the stages of metamorphosis as we adjust to a new phase of our perceptive reality. With an emphasis on circling, we loop through virtual squares like screens and pixels, to the circles of …

Immune Responses Of Neonatal (14-Day Old) And Adult (6 Weeks Old) Mice Lung And Spleen Cells To Gamma-Irradiated Mycobacterium Tuberculosis Stimulation, Oyinkansola F. Adeyemi

Biotechnology Theses

Tuberculosis (TB) caused by Mycobacterium tuberculosis (Mtb) is a global health challenge, especially in vulnerable populations like neonates, whose immune systems are still developing. Understanding the immune responses to Mtb in neonates is critical for the development of better vaccine and treatment strategies. Building upon previous research from our laboratory, which demonstrated that γMtb stimulated neonatal mice (0, 3, and 7 days old) lung cells displayed distinct cytokine profiles and increased expansion of myeloid-derived suppressor cell subpopulations than adult mice lung cells. In the current study, we investigated age-dependent immune responses in 14-day-old (neonates) and 6-week-old (adults) mice. We found …

Farmer's Adaptive Capacity And Adoption Of Strategies To Climate Change Among Mountain Communities In Nepal, Tanvir Hossain

LSU Master's Theses

Climate change presents significant challenges to agriculture worldwide, particularly where farming is central to livelihoods. This phenomenon has been linked to decreasing crop yields due to rising temperatures and reduced rainfall, particularly affecting low-income countries with limited adaptive capabilities. Grasping how farmers perceive climate change is essential for developing effective adaptation strategies to lessen its adverse impacts. This study concentrated on investigating the perceptions and adaptive strategies employed by smallholder farmers in Nepal's Mustang district, utilizing primary data sources. Data from 131 randomly selected households were gathered through structured interviews, while focus group discussions provided qualitative insights into farmers' views …

The Stability Of Epigenetic Variants That Can Act As Loci Causing Phenotypic Change, Raul Faburrieta

Biology Theses

Epigenetic variations are a possible source of heritable phenotypic variation. In this study I focus on phenotypic alterations seen in epigenetic Recombinant Inbred Lines (epiRILs) of Arabidopsis thaliana. These epiRILs allow me to study the effects differentially methylated regions (DMRs) have on phenotypic variance. In a study performed in 2014 by Cortijo et al., they found that DMR’s affect flowering time and root length when grown under greenhouse conditions. In this study, I replicated the Cortijo et al. (2014) study, with some changes, to see whether the same significant eQTL regions are found. I found that, some of the eQTLs …

The Influence Of Stage Of Ripeness On Consumer Perceptions Regarding Selected Aspects Of Non-Astringent Asian Persimmons (Diospyros Kaki Thunb), Robert A. Mirabello Jr

LSU Doctoral Dissertations

Asian Persimmon (Diospyros kaki Thunb.) is an important fruit crop in Asia and in recent decades has experienced expanding production around the world. Non-astringent cultivars are highly desirable in world markets. In the United States the Asian Persimmon is lesser known and consumed although it can be successfully grown in California and the Southeastern United States.This novel study examined the physical/chemical properties of the non-astringent Asian Persimmon ‘Jiro’ grown in Southern Louisiana at three distinct stages of ripeness described as Green (mid-September harvest), Light Orange (mid-October harvest) and Dark Orange (mid-November harvest). Data collected included color, weight, size, soluble …

Lyme Disease In The Northeast: The Unintended Consequence Of Suburban Development, Hayden Neubert

Student Theses 2015-Present

This paper addresses the role of suburban development in the suburbs of the Northeastern United States in increasing human Lyme disease infection. Over the past 50 years, Lyme disease has become the most common vector-borne disease in the United States. In 2021, the two states with the most Lyme disease cases were New York and New Jersey, with a collective 6,524 cases, with Connecticut still representing 541 total cases. It is not a coincidence that case numbers are concentrated in suburban areas with high forest fragmentation. Chapter 1 of this essay analyzes how human land development disrupts bioregulatory ecosystem actors …

How Sensory Exploration Using Expressive Arts Provides A Cohesive Experience For Children With Multiple Diagnoses, Chanelle Goguen

Expressive Therapies Capstone Theses

This thesis looks at the potential benefits and cohesive experience of sensory exploration for children with multiple diagnoses through a trauma informed lens. The researcher was inspired by Prendiville’s (2021) idea of how interactive sensory exploration and art making can help foster and develop reciprocal communication and socio-affective relationships influenced the researcher to design a method that would be used to collect data about potential similar findings. In the method, the interaction of the sensory-specific art mediums were reflected through the expressive therapies continuum (ETC) framework. The research of this thesis acknowledges and explores how trauma and adverse childhood experiences …

Assessing The Use Of Regenerative Agriculture In California Almonds As Climate Change Resilience, Skyler M. Seamons

Master's Projects and Capstones

The agriculture sector is responsible for 10% of the United States’ greenhouse gas emissions. In turn, anthropogenic climate change threatens crops. With its Mediterranean climate, California is the country’s largest agricultural-producing state. Many California crops are at risk due to increasing temperatures and changed precipitation patterns. This paper investigates regenerative farming techniques as a tool to protect California crops from a changing climate. Almonds are used as a case study to analyze the soil management practices, finances, and policies underlying regenerative agriculture in California. A literature review and comparative analysis are used to compare regenerative and conventional soil management practices …

Pfas Exposure And Human Health Risk Management - A Policy Review, Callie Sierra Totaro

Master's Projects and Capstones

For centuries, new chemicals and compounds have been invented to improve material longevity or make a product cheaper to produce. Throughout this time, emerging chemicals have often gone through inadequate testing that may not consider all long term environmental and human health effects before they have gone to market in the United States. As a result, thousands of harmful chemicals have ended up in the food supply, water sources, and everyday items of people in the United States with the negative health effects often not being discovered for decades after the start of circulation. By this point, removal and assessment …

Towards A New Role Of Mitochondrial Hydrogen Peroxide In Synaptic Function, Cliyahnelle Z. Alexander

Student Theses and Dissertations

Aerobic metabolism is known to generate damaging ROS, particularly hydrogen peroxide. Reactive oxygen species (ROS) are highly reactive molecules containing oxygen that have the potential to cause damage to cells and tissues in the body. ROS are highly reactive atoms or molecules that rapidly interact with other molecules within a cell. Intracellular accumulation can result in oxidative damage, dysfunction, and cell death. Due to the limitations of H₂O₂ (hydrogen peroxide) detectors, other impacts of ROS exposure may have been missed. HyPer7, a genetically encoded sensor, measures hydrogen peroxide emissions precisely and sensitively, even at sublethal levels, during …

Optogenetic Induction Of Mitochondrial Oxidative Stress As A Potential Model To Study Non-Hereditary Als, Samia Alam

Student Theses and Dissertations

Mitochondrial dysfunction is a common denominator of neurodegenerative diseases, including hereditary and non-hereditary Amyotrophic Lateral Sclerosis (ALS). Ten percent of ALS cases are hereditary while a majority are sporadic, with no known hereditary link. For effective therapeutic approaches to be developed, there is a need for development of a sporadic animal model to better understand disease mechanism. In previous work, our laboratory developed an animal model (known as mitokiller flies) to study sporadic cases of ALS. The work reported in this honors thesis focuses on the use of optogenetics and electrophysiology recording to study the formation of excessive hydrogen peroxide …

Exploring The Design Space Of Antifungal Peptides, Miryam Kikhwa

Seton Hall University Dissertations and Theses (ETDs)

The synthesis, characterization, and evaluation of antifungal activity of peptides FQ15 and FG10 will be conducted to address the hypothesis. The hypothesis of this thesis is that a peptide with similar properties to AS15 would have similar activity against Cryptococcus neoformans. This study addresses the challenge of drug- resistant microbes by developing inhibitors targeting lipid flippase and exploring their potential as antimicrobial agents against Cryptococcus neoformans. Chapter 1 provides a historical context of the background on the development of antimicrobials and common antifungal treatments, as well as an introduction about C. neoformans and its resistance mechanism. Chapter 2 introduces the …

Impact Of Temperature On Children’S Nutrition: A Comparative Study Of Three Ecological Regions Of Nepal, Prakriti Shakya

Master's Theses

Nutrition obtained during the growth period of childhood significantly influences long-term well-being and overall productivity, ultimately contributing to the economy of a society. However, weather shocks can wreak havoc by damaging crops, changing yields of important crops and disrupting market access, which directly impacts the food intake of both adults and children. When these adverse events occur during childhood, short term and long term inadequacy in nutrition as well as disease incidence can cause malnutrition leading to stunted growth and cognitive impairment that may persist into adulthood, affecting the labor market and increasing health expenditure. To address this issue, we …

From Pixels To Plants: Remote Sensing Of California Invasive Plants, Kenneth Rangel

Master's Projects and Capstones

Invasive plants cause significant impacts to ecosystems, the economy, and human health. California has experienced significant plant invasions and is well suited to future invasion because of its Mediterranean climate and human disturbance. Eradication or control of invasive plant species requires a detailed understanding of their spatial distribution, which typically involves on the ground surveys that can be expensive or inconsistent. Remote sensing offers a potential alternative or supplement to in-person invasive plant mapping. This study performed a comparative analysis of 41 remote sensing studies that mapped the distribution of California invasive plants. I found that while high spectral resolution …

Optics Design And Fabrication For A Lung Interferometry-Radiography System, Rachael Lyn Blair

LSU Master's Theses

Purpose: High prevalence of lung diseases leads to many people worldwide needing spirometry and imaging testing. The typical imaging done uses chest radiography and/or low-dose CT. Lung tissue is difficult to visualize, making them substandard for monitoring lung disease progression. An improvement on these systems is x-ray interferometry which produces additional images that provide better visualization of changes in the lung tissue. The set-up of x-ray interferometry builds upon other imaging systems in that it includes diffraction gratings.

Materials: This thesis focused on implementing the fabrication processes to create diffraction gratings for a lung imager prototype that is currently under …

Screen For Beneficial Genetic And Chemical Modifiers In Drosophila Models Of Als And Traumatic Brain Injury, Will Bonderer

Biological Sciences Theses and Dissertations

The underlying molecular processes of aberrant protein expression in neurodegeneration are intricate and multifaceted, with ribosome-associated quality control (RQC) emerging as a promising avenue of exploration. Ribosome-associated quality control is integral to cellular function. Its evolutionarily conserved pathway encompasses a network of mechanisms designed to ensure the fidelity of protein synthesis, folding, and degradation within the cells of all eukaryotes. The ribosome, central to protein synthesis, plays a pivotal role in this quality control network, and its malfunction can lead to the accumulation of misfolded or aberrant proteins. In the context of neurodegenerative disorders, this dysfunction can have dire consequences. …

The Effects Of Light Cycle Disruption On The Reactive Oxygen Species Production In Zebrafish, Talia Sanders

Honors College Theses

It has been proven that maintaining homeostasis is linked to regulation of circadian rhythms and the light cycles associated with them. Through investigation of zebrafish mitochondria subject to stress via unpredictable light cycle exposure, we previously studied the level of GSK3β protein as it relates to the health of the brain. The data has shown that when a brain undergoes stress, it results in the increased activation of GSK3β which leads to disruptions in circadian rhythm, inflammation, and cell death. Moreso, this overexpression results in reduced ATP production and lowered Complex 1 activity in the mitochondria. However, GSK3β activation is …

Patterns And Potential Mechanisms Of Phenotypic Changes In Urban Small Mammals, Leslie Lopez

Master's Theses

Urbanization is an example of human induced rapid environmental change that can have wide-reaching ecological effects, including habitat destruction, fragmentation, and alteration of local climates. Effects of urbanization have been shown to impact wildlife, as disturbances resulting from urbanization can create novel environments and selective pressures that could lead to changes in morphology, physiology, or both. Small mammals such as rodents are an ecologically important set of wildlife species because they are a key prey item for several predators, hold strong influence over plants as a primary consumer, and some species carry and transmit major human and animal diseases. Previous …

Estrogen Replacement Therapy To Reduce Neurodegeneration And Socio-Cognitive Deficits In A Female Sprague Dawley Rat Model Of Early-Onset Alzheimer’S Disease, Miriam Kirylo

Senior Theses and Projects

No abstract provided.

The Development Of Immunohistochemistry (Icc) And Transcriptomic Protocols To Uncover Neurophysiological Markers Of Stress In Red-Eyed Tree Frogs As A Novel Biome, Shirin Dadina

Senior Theses and Projects

Adverse childhood experiences (ACEs) include various forms of physical and emotional abuse that lead to detrimental long term effects. Agalychnis callidryas (red-eyed tree frogs) have been identified as a novel biomedical model for ACEs. Red-eyed tree frogs demonstrate an escape hatch mechanism under stressors including those from predators, flooding and hypoxia where the embryo undergoes an accelerated developmental process similar to what is observed in the human population regarding precocious puberty. In order to assess this model for neurobiological outcomes of ACEs, morphological and genomic protocols must be developed and validated which is this study’s focus. Paraffin embedding and microtome …

Beyond The Tap: The Flint, Michigan Water Crisis As An Issue Of Environmental Racism, Vincent J. Vitanza

Student Theses 2015-Present

The following paper provides insight into the Flint, Michigan water crisis of 2014 through the lens of environmental racism. The Flint, Michigan water crisis saw the contamination of the municipal water supply of the town of Flint as a result of the decision to switch the main water source from Lake Huron to the Flint River as a temporary measure as the town of Flint switched to the Karegnondi Water Authority contract to supply their municipal water. Flint is a majority minority community, and the facets of race and socioeconomic status played a significant role in how the crisis was …