Life Sciences Commons^™

Ncaa Di Student-Athletes’ Understanding Of And Attitudes Toward Mental Performance Services, Allison Rudisill

Honors Theses

Mental performance services are designed to assist student-athletes with psychological challenges affiliated with performance (McHenry et al., 2022). Currently, only 65 NCAA DI athletic institutions employ a psychological provider, with only 23.2% of those being mental performance consultants (MPCs; Jones et al., 2022). As student athletes are aware of the mental demand of sport (Bemiller & Wrisberg, 2011) and the NCAA begins to bring awareness to the importance of the mental well-being of the athlete (NCAA Transformational Committee, 2023), it is important to understand student athletes’ attitudes toward these services. Therefore, the purpose of this study was to learn about …

The Impact Of Differing Instability Devices On Postural Sway Parameters, Kacey Wallace

Honors Theses

Single-limb balance training is an integral part of preventing and rehabilitating lower extremity injuries. Practitioners use instability devices to provide a progressive overload to an individual during single-limb balance training sessions. Previous investigations have shown that when using instability devices, differences may or may not exist in postural sway parameters during use depending on the specific devices being assessed. Thus, this investigation sought to examine differences between a commonly used foam pad and a novel instability device (block) in measures of postural sway.

This experiment consisted of 22 healthy individuals with no history of lower extremity injury and neurological disorders. …

Understanding Taf13 (Tata Box-Binding Protein-Associated Factor 13) Upregulation In Eukaryotic Cells, Selin Kaplanoglu

Honors Theses

TATA-binding protein (TBP) and TBP-associated factors (Tafs) comprise RNA Polymerase II (RNA Pol II) pre-initiation complex. This universal component carefully controls the transcriptional initiation process. One of the Tafs, Taf13, also plays an important role in the regulation of RNA Pol II transcription initiation which is evolutionarily conserved from yeast to humans. It is found that Taf13 is overexpressed in cancer cells, although the exact mechanism that is responsible for this overexpression is unclear. Our hypothesis suggests that targeted degradation by the 26S proteasome via ubiquitylation [Ubiquitin-Proteasome System (UPS)] may be the mechanism that regulates the stability of Taf13. To …

Associations Between Gut Microbiome Metabolomics And Endometriosis Risk, Progression, And Non-Invasive Diagnosis: A Review, Marlee Sanders

Honors Theses

Endometriosis is a gynecological condition that affects 1 in 10 women of reproductive age, is characterized by growth of endometrial tissue outside of the uterus, and encompasses metabolic, immunologic, and endocrine dysfunction. Despite its significant impact, endometriosis remains inadequately studied within the realm of women's health, emphasizing the crucial need for innovative approaches in the analysis and diagnosis of this complex condition. The cause of endometriosis is unknown however it could be influenced by genetics, environmental factors, diet, lifestyle, and notably composition of the gut microbiome. Recent scholarly interest has sparked inquiries into the correlations between endometriosis and the gut …

Elucidation Of The Overexpression Of Taf2 In Eukaryotic Cells, Morgan Osborn

Honors Theses

Through several studies, Taf2 has been found to be upregulated in various cancer cells. However, the mechanism through which this increased expression of Taf2 occurs remains unknown. As evolutionarily conserved ubiquitin-proteasome system (UPS) maintains protein homeostasis for normal cellular function, we hypothesized that stability of Taf2 may be regulated by this UPS and this UPS may be dysregulated in cancer cells causing overexpression of Taf2. To test our hypothesis, we assessed the role of the UPS in the regulation of the stability of Taf2 by 26S proteasome-mediated degradation. To do so, we performed molecular experiments mainly through two steps: 1st …

Determining The Antimicrobial Activity And Permeabilizing Effects Of Polyunsaturated Fatty Acids And Piscidins On Vibrio Parahaemolyticus And Vibrio Vulnificus, Christina Harris

Honors Theses

Antimicrobial peptides (AMPs) are found in all organisms and have gained attention for their effectiveness against a variety of pathogens. Piscidins are AMPs that were discovered in mast cells of vertebrates, mostly within marine animals, and are an important part of the innate immune system due to their potent antimicrobial properties. Piscidin 1 (P1) and piscidin 3 (P3) are found within sea bass and have different antimicrobial activities. P1 exhibits strong membrane disruptive properties, while P3 goes through the membrane to the targeted DNA. These piscidins can experience metalation with copper (Cu2+) which enhances their antimicrobial activity in vitro (P1-Cu2+ …

The Development Of A New Water-Soluble Zinc Porphyrin, Zntpp--5ap, For Photodynamic Therapy, Kaylee Johnson

Honors Theses

This research focuses on synthesizing a new water-soluble porphyrin with a zinc core that can be used as a photosensitizing agent in photodynamic therapy. Photodynamic therapy is a process in which light is used to activate a photosensitizer to cause cell death. For this study, the porphyrin was synthesized by adding 5-amino-1-pentanol to the ZnTPPC. Once the porphyrin was synthesized it was purified using column chromatography through Sephadex LH-20 and G-50. The structure of the purified ZnTPP-5AP was then analyzed using nuclear magnetic resonance spectroscopy (NMR), infrared spectroscopy (IR), and ultraviolet-visible spectroscopy (UV-vis). Purity of the ZnTPP-5AP was tested using …

An Analysis Of Pollution In Liverpool, Uk, Luke W. Lawson

Honors Theses

Within this experiment the different levels of pollution in areas around Liverpool were tested. These areas were Stanley Park, Lunt Meadows, and Woolton Road. At Stanley Park one of two transects had a geochemical analysis as well as magnetic measurements run to investigate source attribution within the urban environment resulting in data showing high variability in soil properties among the transect. With data supporting evidence of diamagnetic, paramagnetic, and ferromagnetic properties in soil along the same transect. At Lunt Meadows, through the use of XRF for soil samples and nitrate and phosphate testing for water samples, it was found that …

Developing Zntpp-4ab As A Potential Photodynamic Therapy Agent, Kennedy Johnson

Honors Theses

Photodynamic therapy is a fairly new technique used in cancer treatment involving the use of a photosensitizer and light exposure to kill malignant cells. In this research, the use of ZnTPP-4AB as a photosensitizer and red light as a source of radiant light energy was explored in terms of cytotoxicity in the A549 lung cancer cell line. ZnTPP-4AB was synthesized by replacing the carbonyl functional groups on the ZnTPPC porphyrin with 4-amino-1-butanol. Following synthesis, the new porphyrin was purified through the use of Sephadex LH-20 and G-50 in column chromatography in order to remove impurities. To confirm the identity and …

Synthesis Of Zntpp-Il As A Potential Photodynamic Therapy Agent, Addison White

Honors Theses

Photodynamic Therapy is a new technique used in cancer treatment involving the use of a photosensitizer—a porphyrin—and light exposure to kill malignant cells. In this research, ZnTPP-IL was used as a photosensitizer with white light as a possible source of treatment of NIH 3T3 fibroblast and A549 lung cancer cell lines, with preliminary DNA testing on MYC-22 and G-quadruplex samples. ZnTPP-IL was synthesized by the reaction of the porphyrin ZnTPPC with isoleucinol. The new porphyrin was then purified by column chromatography using Sephadex LH-20 and G-50. ZnTPP-IL was characterized through NMR, IR, UV-vis, and fluorescence spectroscopies. The purity of the …

Tree Canopy Cover Influences Habitat Use Of Breeding Birds At Jack Mountain Wildlife Management Area, Kaleigh Thomas

Honors Theses

Across the United States, bird populations have declined due to habitat loss. To better understand habitat use by birds, researchers observed populations at Jack Mountain Wildlife Management Area in southwest Arkansas. Students at Ouachita Baptist University surveyed 94 point count locations to estimate species diversity (total number of species observed) and species abundance (total number of individuals observed). At each point, students recorded the percentage of tree canopy cover, ground cover, midstory cover, and shrub cover. These variables were used as explanatory variables in multiple regression analyses to determine which variables were influential in explaining variation in species diversity and …

4-Week Neuromuscular Training Program On Peak External Power In The Back Squat In Division Ii Collegiate Athletes, Noah Smith

Honors Theses

Neuromuscular training has been shown to have many benefits to athletes ranging from increases in performance to decrease in injury risk by decreasing knee valgus in some athletes. The purpose of this study is to determine if neuromuscular training can influence power in athletes. It is hypothesized that with the intervention program there will be a significant increase in power in the sample. A 4-week neuromuscular training program was implemented with resistance trained athletes (Age: 20.13±.99 yrs., n=14). This training involved balance and plyometric exercises. A pretest and posttest consisted of 2 sets of 6 repetitions of a back squat …

Exploration Of Cytotoxicity Of _Rabidosa Rabida_ Venom On Invertebrate And Vertebrate Cell Cultures, Martina Gooden

Honors Theses

Venom has been observed in thousands of species across different taxa. It can be adapted for different purposes, including prey capture or predator defense. Venom is found almost universally in spiders, but there has been little exploration of spiders that are not medically significant. Rabidosa rabida, the rabid wolf spider, is a common species whose venom has not been adequately investigated. Previous studies indicate that they do not require venom to apprehend easy prey in a laboratory setting, raising a question as to what their venom is adapted to do. To evaluate its effect on mammalian cells, L929 murine fibroblasts …

Structural Phylogenetics Of A Family Dna Polymerases, Yagmur Bingul

Honors Theses

The transition from the RNA to the DNA world stands as an important event, demanding the emergence of enzymatic activities for DNA precursor synthesis, retro-transcription of RNA templates, and replication of single and double-stranded DNA molecules. The distribution of protein families associated with these activities across the three domains of life—Archaea, Eukarya, and Bacteria—adds layers of complexity to the narrative. While Archaea and Eukarya often share similar protein structures, structural biology shows homologous, but distinct, structures in Bacteria. On the other hand, viral polymerases emerge as a crucial part of genome replication and transcription. Their versatility, including RNA-dependent RNA polymerase, …

Heterocycles As Peptide-Based Cleavable Linkers, John Blobe

Honors Theses

As cancer cases continue to rise, the need for advancing the treatment options for cancer is ever increasing. Current cancer therapeutics, while effective at treating localized cancers and a fraction of advanced cancers, generally lack the specificity needed to target more advanced cancers. Recent advancements in cancer treatments have leveraged antibodies to target certain cancers. A class of drugs that utilize antibodies to deliver anti-cancer therapy preferentially to cancer cells, termed antibody-drug conjugates (ADCs), have been quite effective in treating certain advanced forms of certain cancers. These ADCs could be more effective if they could allow for the facile release …

Quantifying The Respiratory Plasticity Of Common Fishes Of The Indian River Lagoon, Logan Exton

Honors Theses

Increasing water temperatures resulting from global climate change introduce new energetic demands for marine organisms. Higher energy input will be required to cope with a subsequently higher metabolic rate, affecting all aspects of an individual’s life and therefore their survival. Because estuaries act as a link between rivers and oceans, they and their inhabitants are considered to be the most threatened by climate change. Therefore, it is crucial to understand how these organisms will respond to increased stressors due to climate change. Checkered pufferfish (Sphoeroides testudineus) are among the most common teleost fish in the Indian River Lagoon, …

The Use Of Vascular Endothelial Growth Factor In Ligament And Tendon Repair, Caroline O'Connell

Honors Theses

The studies in this review have shown that VEGF increases tensile strength, decreases inflammation, and increases vascularization. In studies using exogenous VEGF, it was found that VEGF increases collagen type III fibers, which increases biomechanical strength, and improves graft revascularization. From the research, it can be concluded that endogenous injection of VEGF is more beneficial than exogenous due to endogenous not causing laxity of the connective tissue. VEGF is beneficial when compared to normal post-operative results due to it decreasing the duration of symptoms and decreasing healing time. Using VEGF injection treatments, patients can return to pre-injury activity at a …

Functionalized Gold Nanoparticles And Halogen Bond Interactions With Neonicotinoid Pesticides, Molly Mccuen “Mackey” Sherard

Honors Theses

Neonicotinoid (NN) pesticides have emerged globally as one of the most widely used agricultural tools for protecting crops from pest damage and boosting food production. Unfortunately, some NN compounds, such as extensively employed imidacloprid-based pesticides, have also been identified as likely endangering critical pollinating insects like honey bees. To this end, NN pesticides pose a potential threat to world food supplies. As more countries restrict or prohibit the use of NN pesticides, tools are needed to effectively and quickly identify the presence of NN compounds like imidacloprid on site (e.g., in storage areas on farms or pesticide distribution warehouses). This …

The Complexity Behind The Genetics Of Autism Spectrum Disorder, Júlia Braga Kenyon

Honors Theses

It is suggested that more than 600 genes are associated with ASD. Given the high number of potential ASD-linked genes and that the disorder is a complex spectrum, categorization of the ASD-linked genetic alterations will be useful in the attempt to determine a pattern that can ultimately assist in developing genetic risk factor analysis for the disorder. This thesis aims to analyze the possible cellular and molecular mechanisms that are impacted by such ASD-linked gene alterations and how they may give rise to ASD behaviors. By making this connection, this thesis aims to shine a light on the plausible cellular …

Cd46 Isoforms And Viral Receptor For Adenovirus Type 64d, Corina Stasiak

Honors Theses

Adenovirus Type 64 (Ad.64) belongs to the adenovirus subgroup D, which causes epidemic keratoconjunctivitis (EKC), otherwise known as viral pink eye. There is currently no known effective treatment for EKC. Membrane Cofactor Protein (CD46) is an integral membrane glycoprotein that, in previous studies, has been identified as a protein receptor for the closely related Ad.37. It has been determined that Ad.64 uses CD46 as a receptor on the cell surface in HeLa cells. CD46 is alternatively spliced when expressed to have different isoforms of interest, including the BC and C isoforms. Certain cell types, like A549 lung carcinoma cells, express …

Cd46 Is A Protein Receptor For Human Adenovirus Type 64, Alexander Michael Robertson

Honors Theses

Adenoviruses are important gene delivery vectors and causative agents for a variety of human diseases such as the common cold and gastrointestinal infections. Human adenovirus type 64 (Ad64; formerly 19c) and type 37 are associated with epidemic keratoconjunctivitis. Based upon its high homology and similar disease tropism to Ad37, we hypothesized that Ad64 would have the same protein receptor, CD46 (membrane cofactor protein), as Ad37. We show that a recombinant Ad64 containing an enhanced Green Fluorescent Protein transgene (Ad64.eGFP) enters Chinese hamster ovary cells expressing human CD46 (CHO-CD46) on the surface. Entry into human cervical carcinoma (HeLa) cells is increased …

Development Of A Diaryl Oxazole-Based Cleavable Linker For Peptides, Evan Wolff

Honors Theses

The development of new cleavable linkers increases the diversity of compatible conditions for peptide discovery platforms. Potential applications for these linkers include high-throughput pharmaceutical candidate screening when utilized in Peptide Encoded Libraries (PELs). This thesis describes the development of a bifunctional diaryl oxazole-based cleavable linker that may be incorporated into compounds through Solid-Phase Peptide Synthesis (SPPS). This oxazole-based linker may be rapidly cleaved by cerium ammonium nitrate in aqueous conditions and is compatible with most natural amino acids and a variety of unnatural amino acids. This linker represents the first single-electron oxidant labile linker described to our knowledge and it …

Exercise As A Natural Kappa Opioid Receptor Antagonist To Treat Binge Drinking Withdrawal, Eleanor Wind

Honors Theses

Binge drinking is a major concern, especially among college students. Positive results have been demonstrated in clinical trials that utilized exercise to treat alcohol use disorder, but questions remain regarding the type of exercise and how it should be administered across ages and sexes. Studies using animal models have investigated the neurobiological mechanisms of this treatment to improve understanding of how to implement it. One of the mechanisms under investigation is the endogenous opioid system, specifically the kappa opioid receptor (KOR) subtype. Previous research has demonstrated that exercise treatment concurrent with ethanol exposure attenuated an ethanol-induced increase in KOR receptor …

Acid Attack: How Escherichia Coli Strains Resist Acid Stress, Lindsey Miller

Honors Theses

The systems used by Escherichia coli to combat heightened levels of acidity within their environment are varied and complex. Four distinct systems used by E. coli to resist acid-induced stress were identified in 2004 by Foster (1), though six now are known today. These systems include the glucose-repressed, glutamate-dependent, arginine-dependent, lysine-dependent, ornithine-dependent, and serine-dependent acid resistance systems. Each system is also known by its corresponding abbreviation, AR1-AR6. What is known about each system varies due to identification date, complexity of the pathway, and amount of research completed. Within this paper, each system will be identified and the pathway defined, combining …

Hazards And Perceptions Of Cigarette And E-Cigarette Waste, Jossalyn Rogalski

Honors Theses

No abstract provided.

Violence Against Healthcare Workers, Nichole Osgood

Honors Theses

Workplace violence is any act or threat of physical violence, harassment, intimidation, or other threatening disruptive behavior that occurs at work (US Department of Labor, 2023). There are a handful of different types of workplace violence that include but are not limited to physical violence, verbal abuse and threats, sexual harassment, and psychological trauma (US Department of Labor, 2023). Workplace violence, a multifaceted phenomenon, is not a new concern, but its pervasiveness and intensity in healthcare settings have reached alarming proportions in recent years. In a meta-analysis, it was noted that workplace violence against healthcare workers of any kind was …

We Are The Reason We Stand Still In The Face Of Climate Change., Sydney Hemp

Honors Theses

Many different factors can impact the way any information is received but it is especially important to see how scientific information is received because it is often backed up by a great deal of evidence, especially in the case of global climate change. Many of these factors have nothing to do with science and everything to do with social influences, political parties, and other demographic measures. Discussion of these relationships is important when discussing communication in a broad sense, but it can be even more important when bringing up things like global climate change, which has become a political topic …

The Unexpected Significance Of Myosin A In Organization Of M-Line Protein Unc-89/Obscurin Within Striated Muscle Cells Of Caenorhabditis Elegans, Kar Men Lee

Honors Theses

This research investigates the unexpected significance of Myosin A in the organization of M-line protein UNC-89/obscurin within striated muscle cells of the nematode Caenorhabditis elegans (C. elegans). The study employs immunofluorescence techniques to examine the spatial and temporal dynamics of UNC-89/obscurin and Myosin A during embryonic development. The sarcomere, a fundamental unit in muscle cells, is governed by the 'sliding filament model,' where the M-line serves as a crucial anchor for Myosin A. This research seeks to understand the relationship between muscle function in humans and C. elegans, utilizing the latter's simple anatomy and genetic tractability. The …

Diaryl Oxazoles As Cleavable Linkers For Drug Discovery Platforms, Elizabeth Taggart

Honors Theses

Within the field of medicine and pharmacology, discovering small molecule or biologic based molecules with therapeutic potential is a difficult task. Current methods involve individually screening hundreds of compounds on a potential target biomolecule, and recent technologies have explored peptide encoded libraries (PELs) as a means of making this screening process more high-throughput. These libraries produce a large number of small molecule drug candidates each conjugated to a unique peptide fragment, functioning as a barcode. Analysis of PELs requires the capture of hit small molecules and the subsequent release of their peptide tags; however, current approaches are limited in their …

Odorant Binding Causes Cytoskeletal Rearrangement, Leading To Detectable Changes In Endothelial And Epithelial Barrier Function And Micromotion, Annabella Nilon

Honors Theses

Non-olfactory cells have excellent biosensor potential because they express functional olfactory receptors (ORs) and are non-neuronal cells that are easy to culture. ORs are G-protein coupled receptors (GPCRs), and there is a well-established link between different classes of G-proteins and cytoskeletal structure changes affecting cellular morphology that has been unexplored for odorant sensing. Thus, the present study was conducted to determine if odorant binding in non-olfactory cells causes cytoskeletal changes that will lead to cell changes detectable by electric cell-substrate impedance sensing (ECIS). To this end, we used the human umbilical vein endothelial cells (HUVECs), which express OR10J5, and the …