Life Sciences Commons^™

Valorization Of Rice Bran For Functional Film Fabrication Using Cold Plasma For Food Packaging And Biomedical Applications, Saydul Md Safwa

Graduate Theses and Dissertations

Abstract Over the past few years, conventional plastic packaging materials have become increasingly concerned with their environmental impact. Therefore, the demand for bioplastics has been increasing significantly over the years. Rice milling, a process that modifies paddy rice into edible rice, generates by-products such as rice bran. Rice bran has cellulose, hemicellulose, lignin, and protein, which makes it a potential material for bioplastic fabrication. However, like most biodegradable films, rice bran films fall short when it comes to practical packaging applications. They often lack the strength, flexibility, moisture resistance, and barrier performance needed to compete with synthetic plastics. Most importantly, …

Seasonality Of Bovine Respiratory Disease Incidence, Nasal Temperatures, And The Nasal Microbiome In Receiving Beef Cattle: Understanding The Risk Of The Bovine Respiratory Disease Complex, Robin Ashley Cheek

Graduate Theses and Dissertations

Bovine respiratory disease (BRD) has persistently been the leading cause of morbidity of beef cattle in the United States. Season has been directly associated with respiratory disease incidence and is likely a proxy for weather conditions, which vary by geographical location. The nose is one of the first points of contact between the outside environment and inside the body. The nasal microbiome and its relationship with BRD have been heavily studied within beef cattle, but there are not yet any studies to determine if the environment within the nasal cavity has a role to play in this mechanism. This dissertation …

Soilless Substrates As A Critical Control Point For Mitigating Food Safety Risks Within Hydroponic Leaf Lettuce Production, Eric Nuertey Tetteh

Graduate Theses and Dissertations

The effect of hydroponic soilless substrates and microbial biocontrol agents on the persistence of foodborne pathogens in indoor lettuce production is variable depending on substrate type, pathogen species, and biocontrol formulation. This study addressed key gaps by first characterizing the ability of different substrates to support Salmonella and Listeria monocytogenes survival and then evaluating the ability of pine biochar and Bacillus subtilis to suppress pathogen persistence in selected substrates under controlled conditions. In the first experiment, the persistence of Salmonella and L. monocytogenes was evaluated in four soilless substrates: rockwool (RW), low density foam (LDF), peat moss (PM) and coco …

Characterization Of River Cane Morphology, Density And Biomass In Sun And Shade In Stream Riparian Zones In Northwest Arkansas, Emily Shy-Anne Carter

Graduate Theses and Dissertations

River cane, or Arundinaria gigantea Walter (Muhl.), ecosystems serve as ecological and cultural keystones, offering wildlife habitat, streambank stabilization, and construction/craft material for Native groups who utilize river cane. However, these ecosystems have declined substantially because of overgrazing, land conversion, and fire suppression, resulting in reduced patch size and culm morphology. Historically widespread across floodplains and riparian zones, canebrakes, or monotypic forests of river cane, have been restricted to small fragments. This study evaluated how canopy cover influences light availability, culm morphology, density, and biomass of river cane across eight riparian sites in Northwest Arkansas to inform restoration efforts. Light …

Exploring Primary Care Providers’ Compassionate Care And Their Evidence-Based Practice With Death, Grief, And Loss: Patient Experience And Perspective, Kathryn Krzykala

Dissertations

Research on primary care providers’ (PCPs) education regarding Death, Grief, and Loss (DGL) remains limited. Specifically, data is lacking regarding patients' perceptions and opinions on what they need and expect from their PCPs regarding grief and loss. This exploratory mixed-method study comprised 146 participants who had experienced death and non-death loss. It examined participants' perceptions of 1) PCP compassion, 2) PCP utilization of evidence-based grief and loss practices, 3) their own level of comfort discussing DGL with their PCPs during medical visits, and 4) their personal grief and loss experiences. The findings revealed that PCPs' grief-informed evidence-based treatment, as reflected …

Environmental Tolerances And Habitat Association Of Three Endemic Crayfish Species In Texas, Andrew Walker

Biology Theses

Little is known about many of the 52 crayfish species in Texas. This thesis investigates physiological tolerances of three species endemic to the Jackson Vale ecoregion of Southeast Texas, the Bastrop crayfish (Procambarus texanus), Nueces crayfish (Procambarus nueces), and the Fayette’s crayfish (Procambarus fayettei) as well as the White River crayfish (Procambarus acutus), which is ubiquitous throughout East Texas. Thermal, hypoxia, and acidity tolerances were tested on wild caught individuals through laboratory assays. Findings from this study will inform wildlife managers of future conservation strategies relating to these endemic species.

Exploring Staphylococcus Aureus Host-Pathogen Interaction Using Proteomic Technologies, Emilee Marlene Elizabeth Mustor

USF Tampa Graduate Theses and Dissertations

Staphylococcus aureus deploys an arsenal of virulence effectors to remodel the host environment during infection, while simultaneously the host mounts an immune response to contain and eliminate this bacterium. This dynamic interplay defines the host-pathogen interface and shapes infection outcome. In this context, the secreted proteases represent a major arm of S. aureus pathogenesis, promoting bacterial persistence in the host by dismantling immune defenses, cleaving host signaling molecules and reshaping the host proteome. However, traditional substrate-by-substrate characterization cannot reveal the full scope of proteolytic activity occurring during infection. To address this, we developed Terminal Amine-Guanidination of Substrates-Charge Reversal (TAGS-CR), a …

Investigating The Role Of Cellular Senescence On Vestibular Dysfunction In Repetitive Mild Traumatic Brain Injury, Michael Volyanyuk

Dissertations

Vestibular dysfunction is a prevalent and persistent consequence of mild traumatic brain injury (mTBI). Clinical reports reveal that women experience vestibular deficits more frequently than men following mTBI. Despite the high prevalence and debilitating nature of vestibular symptoms, preclinical literature evaluating mTBI-associated vestibular impairments is limited. Emerging evidence demonstrates that cellular senescence is involved in the pathological molecular sequela of mTBI. It has been shown that senescence contributes to cognitive deficits following repetitive mTBI (rmTBI); however, its role in rmTBI-associated vestibular dysfunction remains unexplored. This body of work investigated the role of cellular senescence on rmTBI-induced vestibular dysfunction in both …

House Sparrow Invasion Success Across Ecological, Immunological, And Molecular Scales, Kailey Marie Mccain

USF Tampa Graduate Theses and Dissertations

Biological invasions offer natural experiments for studying how organisms cope with ecological novelty. Although invasion success is often attributed to enemy release, introduced organisms do not enter enemy-free environments. Instead, they enter heterogeneous landscapes in which some familiar enemies are absent, others remain, and entirely novel enemies may be encountered. The question, then, is how invaders (hereafter non-natives) are poised to respond to these threats? This dissertation examines whether successful invasions in house sparrows (Passer domesticus) is associated with shifts in pathogen exposure, immune dynamics in blood, gut physiology, and gene co-expression network structure.

Chapter 1 is at the population/ecological …

Association Of Social Isolation, Loneliness, Social Strain And Support With Cancer Onset, And Frailty And All-Cause Mortality In People With Cancer, Joanne Elayoubi

USF Tampa Graduate Theses and Dissertations

Cancer is the second leading cause of death both in the United States and globally and for individuals over sixty-five. In 2026, approximately 2,114,850 new cancer cases are projected to emerge according to the National Center for Health Statistics (Siegel et al., 2026). Cancer incidence is increasing, making older adults sixty-five and older at risk of frailty and mortality following cancer. Although social isolation, loneliness, lack of social support, and social strain have been found to be risk factors for poor health and mortality, it is not clear the extent to which social relationships are associated with incident cancer, and …

Understanding The Needs And Practices Of Cut Flower Growers In Louisiana, Jessie M. Hoover

LSU Doctoral Dissertations

The specialty cut flower industry is expanding nationwide, including southeastern states such as Louisiana. Existing studies and market analyses focus on national trends of other regions with established floriculture networks, leaving a critical gap in understanding the unique challenges and opportunities faced by growers in Louisiana. This lack of data limits the ability of extension professionals, policymakers, and industry stakeholders to develop targeted strategies that support local production and market development. This study used qualitative case study methods to investigate the practices, challenges, and needs of 13 specialty cut flower growers in Louisiana. The study utilized Urie Bronfenbrenner’s (1979, 1986) …

Cap1 (Cyclase-Associated Protein 1) Mediates The Camp Regulation Of Adhesion And Proliferation Of Schwann Cells In The Peripheral Nervous System, Jarna Karmoker

Student Theses and Dissertations

The peripheral nervous system relays signals between the central nervous system and the rest of the body, and peripheral nerve injuries and neuropathies can cause permanent neurological deficits. Schwann cells (SCs) are glial cells that myelinate neurons, which is essential for neuronal function and nerve repair. CAP1 (Cyclase-Associated Protein 1) is best characterized as an actin-regulating protein, while it also regulates other cellular processes, including matrix adhesion, migration, and proliferation. We discovered that cAMP-induced CAP1 dephosphorylation regulates relevant cell functions. While cAMP is also important for SC function and myelination, the underlying mechanisms remain largely unknown. We silenced CAP1 in …

Fear Of Negative Evaluation And Intrinsic Motivation In Resistance-Trained Individuals: A Comparison Of Novice And Experienced Lifters, Karter Bokker

Student Theses and Dissertations

This study examined the relationship between fear of negative evaluation (FNE) and intrinsic motivation in resistance-trained individuals and compared FNE levels between novice and experienced lifters. Twenty-two male participants (n = 8 novices, n = 14 experienced) completed questionnaires assessing FNE and intrinsic motivation. A Pearson correlation and independent-samples t-test were conducted. Results indicated no significant relationship between FNE and intrinsic motivation (r = -.144, p = .523). However, novice lifters reported significantly higher FNE scores than experienced lifters, (t20) = 2.71, p = .014, with a large effect size (d = 1.20). These findings suggest that while FNE may …

Assessing The Effectiveness Of Wetland Reserve Easements As Habitat For Birds In The Mississippi Alluvial Valley Of Eastern Arkansas, Colin K. Dobson

Student Theses and Dissertations

Globally, wetland habitats suffered major declines during the 20th century. In the 1990s, the United States government created the Wetland Reserve Program (later consolidated into the Agricultural Conservation Easement Program), to combat the loss of wetlands. Despite success in attracting landowners, minimal monitoring of properties enrolled into the program (easements) has occurred. In this study, we monitored avian communities at easements, compared avian diversity at easements to agriculture and bottomland forests, and quantified microhabitat, site-specific, and surrounding landscape features at easements. We also studied the demographic rates of an indicator species of bottomland forests, the Prothonotary Warbler (Protonotaria citrea) at …

Efficacy Evaluation Of An Allopathic & Homeopathic Anthelmintic On Small Ruminant Parasite Loads, Kennon Leigh Harrington

Student Theses and Dissertations

This study aimed to compare the efficacy of an allopathic and homeopathic anthelmintic in sheep and goats naturally infected with internal parasites. Animals with a threshold of ≥1000 EPG (n=30) were enrolled. The allopathic group received a single 14.79 mL per 50 lbs. BW drench solution of Prohibit®. The homeopathic group received a mixed herbal aqueous suspension, 2x/day D1-D3, thereafter 2x/day once a week W1-W4. There was an effect of anthelmintic treatment (P=0.0234) and day (P=0.0116) on total EPG, but no interaction effect for treatment by day (P=0.2440). For Initial sampling, there was a weak correlative tendency between weight and …

Reproductive Trends Of Male Atlantic Spotted Dolphins (Stenella Frontalis) Following Two Hurricanes In The Bahamas, Tara Southerland Pedersen

USF Tampa Graduate Theses and Dissertations

Marine mammals are impacted by hurricanes that may influence reproductive success and genetic diversity. Understanding how populations respond to demographic change is increasingly important as extreme weather events become more frequent. A population of Atlantic spotted dolphins (Stenella frontalis) on Little Bahama Bank in the Bahamas has been observed continuously since 1985. In 2004, two hurricanes struck the region within one month, resulting in the loss of approximately 36% of the population. Population declines can alter the operational sex ratio (OSR), social structure, and encounter rates among potential mates, potentially influencing patterns of reproductive success. Previous molecular studies showed that …

Describing Propagation Of Carbapenem-Resistant Acinetobacter Baumannii Through Comparative Genomics And Phenotypic Analysis Of Modern-Day Clinical Isolates, Emma Graffice

ETDs from 2020-2029

Understanding the genomic structure of bacterial populations is essential for tracking antimicrobial resistance and informing public health strategies to prevent spread of multidrug resistant organisms. In this study, we investigated the diversity and distribution of carbapenem-resistant Acinetobacter baumannii (CRAb) lineages across a major healthcare system in the U.S. Deep South through both retrospective and prospective isolate collection. Our analysis revealed a heterogeneous population composed of multiple genotypically and phenotypically distinct lineages. To improve the resolution of lineage classification, we applied average nucleotide identity (ANI) as a high-resolution metric for comparative genomics. A ≥99% ANI threshold effectively delineated lineages, outperforming traditional …

Contribution Of Phosphorylation To Hp1a Function, James Walts

ETDs from 2020-2029

Heterochromatin Protein 1 (HP1) family members are highly conserved, non-histone chromosomal proteins essential for heterochromatin formation and transcriptional regulation. In Drosophila melanogaster, HP1a functions both as a transcriptional repressor and activator, as well as other functions, and undergoes post-translational modifications, including phosphorylation. The functional consequences of HP1a phosphorylation remain largely unexplored. This thesis investigates the role of phosphorylation at serines 89/90/91 (S899091) of HP1a by generating two mutant alleles: a phospho-mimic (SE) and a phospho-block (SA). Both alleles produce stable mRNA transcripts; however, Western blot analysis revealed reduced protein accumulation relative to wildtype HP1a. Homozygous phospho-block animals are embryonic lethal …

Jnk Signaling Impacts Car-T Cell Effector Function, Charles Kuhlmann

ETDs from 2020-2029

The potent cytotoxic activity of T cells serves as a natural defense against cancers. Chimeric antigen receptor (CAR) T cell therapy represents a strategy for harnessing T cells against advanced tumors. CAR-T cell therapy has seen striking success against hematologic malignancies, prompting its application to new diseases. Ovarian cancer is a highly lethal disease characterized by frequent recurrence and aggressive nature, prompting the investigation of CAR-T cell therapy as a salvage regimen. However, like in other solid tumors, the early performance of CAR-T cell therapy in ovarian cancer is poor. While many strategies exist for improving the longevity of CAR-T …

Investigating The Role Of Centromere Deletion In Chromosome Segregation, Azait Imtiaz

ETDs from 2020-2029

Centromeres are critical genomic regions responsible for ensuring accurate chromosome segregation during cell division. Disruptions in the structural or functional integrity of centromeric DNA could impair kinetochore assembly, leading to chromosome missegregation and chromosomal instability (CIN), a hallmark of various human diseases, including systemic sclerosis (SSc). This study investigated the contribution of centromeric DNA damage and deletion to CIN and explored potential pathogenic mechanisms relevant to SSc. We utilized hTERT-immortalized fibroblast cell lines (CHON-002 and BJ-5ta) to implement two complementary approaches: (i) treatment with bleomycin, a chemotherapeutic agent known to induce DNA double-strand breaks (DSBs), and (ii) targeted deletion of …

A Pilot Study Of The Acute Effects Of Isometric Exercise And Aerobic Exercise For Improving Blood Pressure And Vascular Hemodynamics In Hypertensive Adults, Ayih Cedric Edoh

ETDs from 2020-2029

Hypertension (HTN) is one of the most prevalent and modifiable risk factors for developing cardiovascular disease (CVD). Aerobic exercise is a well-known method to lower blood pressure (BP), but recently, isometric exercise has been purported to lower BP to a similar or greater extent. There is little known about the effects of isometric exercise on acute BP and 24-hour ambulatory blood pressure (ABP). Therefore, the purpose of this pilot study was to compare the effects of isometric and aerobic exercise on improving vascular hemodynamics and 24-hour ABP in hypertensive adults. METHODS: Seven participants (age 25-65 years) with a diagnosis of …

Histone Deacetylases In Kidney Injury: Distribution, Injury-Specific Regulation, And Fibroblast Hdac1 Function, Huy Nguyen

ETDs from 2020-2029

Acute kidney injury (AKI) is increasingly recognized as a major contributor to the development of chronic kidney disease (CKD) through maladaptive repair and interstitial fibrosis. Epigenetic regulation has emerged as a critical factor of kidney injury responses, however the injury-specific and cell-type specific roles of histone deacetylases (HDACs) remain poorly defined. This dissertation investigates the regulation and functional significance of HDACs in kidney injury, with a particular focus on fibroblast HDAC1 as a mediator of interstitial fibrosis development following AKI. In my cisplatin-induced AKI model, a single dose of cisplatin caused a significant decline in kidney function in both male …

From Cardiovascular Safety Assessment To Peptide Hemolytic Activity Prediction: Integrative Computational Analyses For Drug Evaluation And Discovery, Xinran Zhu

USF Tampa Graduate Theses and Dissertations

This thesis applied integrative computational approaches to two major areas of drug evaluation and discovery: cardiovascular safety assessment and peptide hemolytic activity prediction. First, pharmacovigilance data from the FDA Adverse Event Reporting System (FAERS), electronic health records (EHRs) from TriNetX, and transcriptomic analyses were combined to evaluate anesthetic-associated cardiac rhythm adverse drug events. Among propofol, ketamine, dexmedetomidine, and midazolam, dexmedetomidine showed the strongest association with bradycardia across both FAERS and propensity score-matched EHR analyses. Stratified analyses further suggested substantially higher bradycardia prevalence among older patients and a modestly increased risk among male patients. Drug-drug interaction analyses identified risperidone and albuterol …

“He’S Got The Whole World In His Hands”: Seed Sovereignty And Agricultural Decision Making In Southeastern Kenya, Caroline Stahley

USF Tampa Graduate Theses and Dissertations

Increasing corporate control of the global seed supply and restrictions to local exchange threaten the autonomy of smallholder farmers and Indigenous communities. This project contributes to agricultural sovereignty movements by examining the decisions farmers make to address climate uncertainty, market volatility, and development agendas. Specifically, how is seed saving practiced and understood in relation to sovereignty? It reimagines what global food systems might look like if investment were directed to producers rather than intermediaries, arguing that cultivators lack support, not knowledge or capacity. Across these domains, seeds act as a motif to describe the concerns, innovations, and visions of farmers.

House Sparrows Reveal Hidden Reservoirs Of Antimicrobial Resistance, Nathan Dowling

USF Tampa Graduate Theses and Dissertations

Antimicrobial resistance (AMR) is a growing global health threat, yet surveillance for antimicrobial resistance genes (ARGs) remains concentrated in clinical and livestock systems, leaving environmental and wildlife reservoirs comparatively understudied. The objective of this study was to evaluate whether one synanthropic bird species integrates landscape-level antimicrobial resistance pressures across socioeconomic gradients and to identify the predictors most strongly associated with cross-national variation in ARG prevalence. We examined ARG occurrence in the globally distributed house sparrow (Passer domesticus), sampling 205 individuals across eight countries spanning large gradients in economic development, antimicrobial use, and governance structure. Using targeted molecular screening of gastrointestinal …

Amending Planting Media With Typha-Derived Biochar For Sustainable Horticulture, Elissa Touma

USF Tampa Graduate Theses and Dissertations

Constructed wetlands are widely used to improve water quality by removing excess nutrients from wastewater and stormwater. Nutrients are stored within wetland vegetation, particularly in fast-growing plants such as Typha. Harvesting vegetation provides an opportunity to permanently remove these nutrients while generating biomass suitable for beneficial reuse. Converting harvested biomass into value-added products such as compost and biochar can support nutrient recovery and sustainable biomass management. In addition, increasing concerns regarding the environmental impacts of peat extraction have created interest in renewable alternatives for horticultural growing media. Therefore, the objective of this study was to evaluate the potential of harvested …

Elucidating The Mechanisms Of Complex Flavoprotein Dehydrogenases, Tyler Alt

Dissertations

Flavins are ubiquitous redox active cofactors that can catalyze electron transfer between substrates. They offer the advantage of being able to stabilize a range of oxidation and protonation states, as well being amenable to covalent modification, which can further alter their activity. As such, the scope of reactions catalyzed by flavoproteins is broad and flavin has implications in a range of biological processes. The two predominant forms of flavin that serve as redox active cofactors are flavin adenine mononucleotide (FMN) and flavin adenine dinucleotide (FAD), which are functionally homologous. A major complication of studying flavoprotein dehydrogenases, and one that has …

Assessment Of Artificial Oyster Reef Health Based On Water Quality, Oyster Recruitment, And Perkinsosis Infection In Tampa Bay, Christian J. Goldman

USF Tampa Graduate Theses and Dissertations

Providing nursey habitat, regulating nutrients, stabilizing shorelines, increasing biological diversity, improving water quality, and creating three-dimensional environments are some of the ecosystem services of oyster reefs. Oyster reefs are threatened by overharvesting, changes in water quality, pollution, and disease. The Eastern oyster, Crassostrea virginica, is the primary oyster species along Florida’s Gulf coast. This study aimed to evaluate the relationship between multiple parameters on artificial reefs and how they influence the health of these reefs. Here, young (less than five years old) artificial oyster reefs were monitored for one year across three sites in Tampa Bay, FL: Lassing Park (LAS), …

A Rho5-Cnc1-Dnm1 Signalling Module Coordinates Multi-Organelle Execution Of Regulated Cell Death In Yeast, Justin Bauer

Theses and Dissertations

Regulated cell death is a conserved process with major implications for health and disease, yet how yeast cells integrate stress signals to determine survival versus death remains unclear. This thesis investigates Cyclin C (Cnc1), a conserved Mediator kinase module component with both transcriptional and mitochondrial roles. Together with Cdk8 Cnc1 primarily represses stress response genes, while upon stress it translocates to the mitochondria to induce mitochondrial fragmentation with the dynamin-related GTPase Dnm1. Using structure-guided separation-of-function mutants, I demonstrate that Cnc1-dependent death requires its mitochondrial function rather than its transcriptional role, and that its effector Dnm1 acts as a signaling node …

Quantitative Determination Of Nano Delivered Kras Sirna, Axel S. Deraspe

USF Tampa Graduate Theses and Dissertations

This study focuses on the development and validation of analytical methods for the detection of therapeutic KRAS-targeting siRNA in biological systems. Physicochemical characterization showed that p5RHH-formulated siRNA nanoparticles exhibit a controlled nanoscale size distribution, moderate polydispersity, and a negative zeta potential, suggesting colloidal stability and potential suitability for cellular uptake. Scanning electron microscopy confirmed a predominantly spherical morphology with a relatively uniform size distribution, consistent with dynamic light scattering measurements.

To enable accurate siRNA quantification, stem–loop reverse transcription (SL-RT) coupled with SYBR Green–based quantitative Polymerase Chain Reaction (qPCR) was optimized. Key parameters, including a primer concentration of 100 nM and …