Life Sciences Commons^™

Physiology And Genetics Shape The Microbiome Of A Seabird Species (Oceanodroma Leucorhoa) More Than Environmental And Social Factors, Douglas Steven Pearce

Masters Theses

The microbiome provides multiple benefits to animal hosts that can profoundly impact health and behavior. Microbiomes are well-characterized in humans and animals in controlled settings, yet assessments of wild bird microbial communities remain vastly understudied. This study examines the multiple factors that affect the microbiome of a burrow-nesting Procellariiform bird species, Leach’s storm-petrel. 16S rRNA-based Illumina Mi-Seq analyses are used to assess the composition and structure of bird and burrow-associated bacterial communities. Results indicate that sex and skin site contribute to bird-associated bacterial community variation, and MHC heterozygosity impacts these bacterial assemblages in a sex and site-specific manner, potentially having …

From Land To Lake: Contrasting Microbial Processes Across A Great Lakes Gradient Of Organic Carbon And Inorganic Nutrient Inventories, Deborah K. Dila

Masters Theses

Freshwater ecosystems have strong linkages to the terrestrial landscapes that surround them, and contributions of carbon and inorganic nutrients from soil, vegetation and anthropogenic sources subsidize autochthonous water body productivity to varying degrees. Abundant freshwater phytoplankton and bacterioplankton are key to linking the planet's geosphere and atmosphere to the food webs in the hydrosphere through their growth and respiration. Rich resources that move through land margin waterways make them active sites for cycling organic carbon and thus important, but understudied, contributors to global climate. During 2010-2011, we examined seasonal changes in carbon and nutrient inventories, plankton community composition and metabolism …

Trophic Transfer Of A Naturally Occurring Algal Toxin From A Freshwater Lake To Little Brown Bats, Devin Jones

Masters Theses

Microcystis aeruginosa is a species of cyanobacteria capable of producing a hepatotoxin called microcystin. As toxic M. aeruginosa overwinters in the sediments of lakes, it is ingested by some mayfly larvae, such as those of Hexagenia spp., and thus

microcystin bioaccumulates in these insects. When Hexagenia emerge from lakes to

reproduce, they provide an abundant, albeit temporary, food source for many terrestrial

organisms such as bats. Little brown bats, Myotis lucifugus, likely feed opportunistically

on aquatic insects. To test if microcystin moves from aquatic to terrestrial ecosystems via trophic transfer, we 1) tested bat feces for the presence of …

Green And Choking: Exploring The Dynamics Of Seasonal Productivity And Hypoxia In A Great Lakes Area Of Concern And Habitat Blueprint Estuary Using Time-Series Measurements, Anthony D. Weinke

Masters Theses

Although hypoxia (dissolved oxygen <4 mg L-1) in the bottom waters of lakes, reservoirs, and estuaries may be a natural product of stratification in eutrophic or mesotrophic systems, there is increasing concern because the occurrence of hypoxia is spreading in many areas where hypoxia did not previously exist. Part of this new knowledge can be attributed to time-series data from buoy observatories that monitor systems in good weather and in bad, and allowing insight into the inner workings of a lake where sampling only a few times per year would not. This study made use of a five-year time-series of meteorological and water quality data in order to examine the effect of episodic wind-events on stratification and hypoxia within Muskegon Lake, Michigan, as well as performing bi-weekly lake-wide monitoring to evaluate the effects of stratification, hypoxia, and wind-events on the lake. In the wind-event portion of the study, we found that events where wind speeds were above average for an extended period of time occurred fairly frequently on the lake, but that thermal stratification allowed only the strongest events to significantly mix the water column at the buoy location a few times per year. This provided infrequent relief of hypoxia in the bottom waters. The second portion of the study found extensive effects on the water quality and biology in the bottom of the lake due to hypoxia such as increased phosphorus concentrations, enhanced phycocyanin following a strong windevent, and decreased fish abundance, richness, and size. We also found that hypoxia occurred lake-wide at all four sample locations, but was most stable at the deepest point in the lake that was least influenced by wind-events. It is possible that the combination of hypoxia and strong episodic wind-events leads to entrainment of phosphorus-rich waters to the surface initiating or continuing an algal bloom. Hypoxia and wind-event mediated internal loading of phosphorus could be a positive feedback loop for cyanobacterial blooms and hypoxia in Muskegon Lake.

Examining The Effect Of Climate Change On The Upper Mesophotic Coral Montastrea Cavernosa (Linnaeus 1767), John Skutnik

Masters Theses

Coral reefs are under increasing pressure from global climate change. In particular, ocean warming is having a deleterious effect on many of the world’s shallow reefs. Some authors suggest that acute exposure is more detrimental than chronic, versus others who indicate the opposite. However, little knowledge exists regarding heat induced stress on deeper mesophotic reefs. Here, I examined the effect of acute (72 hrs.) and chronic (480 hrs.) heat stress using laboratory experiments on coral Montastraea cavernosa (Linnaeus 1767) collected from an upper mesophotic (~30 m) reef off Islamorada Florida. I examined a variety of putative immune and stress genes …

Evaluating Foraging Habits, And Estimating Prey Consumption, And Growth Of Brook Trout In A Coolwater Michigan Stream, Graeme R. Zaparzynski

Masters Theses

Brook trout (Salvelinus fontinalis) are a cold water ectotherm whose native distribution includes most of eastern Canada, the upper Midwestern United States, and the eastern and mid- Atlantic United States as far south as Georgia. The native range of brook trout in the United States has been reduced as a result of interspecific interactions with introduced species, and anthropogenic land use changes that drive water temperature changes in many of their native watersheds. Anthropogenic land-use changes tend to increase thermal regimes of aquatic systems, affecting brook trout physiology and limiting them to colder, headwater reaches. The threat of …

Variation In Alkaloid Defenses Of The Dendrobatid Poison Frog Oophaga Pumilio Lead To Differences In Avaoidance By Arhtropods, Sarah Bolton

Masters Theses

Conspicuously colored dendrobatid frogs sequester alkaloid-based defenses from dietary arthropods, resulting in considerable alkaloid variation among populations. Although alkaloids act as a defense against predation, relatively little is known about how alkaloid variation is perceived and functions as a defense against predators. Throughout its geographic range, previous studies have found the dendrobatid frog Oophaga pumilio to have particularly variable alkaloids, and that differences in these alkaloids are associated with differences in toxicity to laboratory mice. Although toxicity is one measure of alkaloid variation, predator avoidance of dendrobatids might simply be due to the bitter or unpalatable nature of alkaloid defenses. …

Sequestered Alkaloid Defenses In The Dendrobatid Poison Frog Oophaga Pumilio Provide Variable Protection From Microbial Pathogens, Kyle Hovey

Masters Theses

Amphibians produce the majority of their defensive chemicals, however alkaloid defenses in poison frogs are sequestered from dietary arthropods. Alkaloids function as a defense against predators, and certain types appear to inhibit microbial growth. However, alkaloid defenses vary considerably among populations of poison frogs, reflecting geographic differences in availability of dietary arthropods. Consequently, environmentally driven differences in poison frog alkaloid defenses may have significant implications regarding their protection against pathogens. While natural alkaloid mixtures in poison frogs have recently been shown to inhibit growth of non-pathogenic microbes, no studies have examined the effectiveness of alkaloids against microbes that infect frogs. …

Heterochrony Of Cranial Bones In Amniota And The Phylogenetic Placement Of Testudines, Kathleen Sagarin

Masters Theses

The study of developmental systems may help to resolve the disagreement between morphological data and molecular data when it comes to the placement of Testudines among Amniota. Among other unique morphological adaptations, turtles possess an anapsid (unfenestrated) condition of the temporal region of the skull. If turtles are descended from diapsids, as molecular data suggests, this implies a rapid transformation of the temporal region from the diapsid condition to the anapsid condition. This study specifically addressed temporal bone heterochony among amniotes using the methods of Continuous Analysis (Germain and Laurin 2009) and Parsimov-based Genetic Inference (Harrison and Larsson 2008) to …

Orrm6 Is A Novel Organellar Rna Recognition Protein Involved In Plastid Rna Editing In Arabidopsis Thaliana, Justin B. Hackett

Masters Theses

RNA editing is a conserved mechanism of post-transcriptional modification in eukaryotes that changes specific nucleotides in RNA transcripts. In flowering plants, this is limited to cytidine to uridine deaminations. Plastid RNA editing events are critical for plastid development and the loss of editing can result in untranslated or non-functional proteins. There have been 40 RNA editing events discovered in the Arabidopsis thaliana plastid. Recently organellar RNA recognition motif (ORRM) proteins have been shown to broadly affect plastid RNA editing and interact with sequence specific trans-factor pentatricopeptide repeat proteins (PPR). ORRM proteins contain a canonical RNA recognition motif that has been …

Detection And Attribution Of Long-Term Vegetation Changes In Northern Alaska, Robert Barrett

Masters Theses

Climate change is impacting terrestrial ecosystems world-wide and the Arctic is particularly vulnerable as it is warming faster and with greater magnitude than other regions. Understanding the responses of arctic plants species to abiotic factors is crucial to predicting the impact climate change will have on the Arctic because plants play critical roles in carbon exchange, energy balance, and trophic interactions. Using data from long-term research sites in Barrow and Atqasuk, Alaska, the purpose of this thesis was to investigate how arctic plants respond to 17-19 years of experimental warming, establish the relative strengths of various abiotic factors in predicting …

Investigating Ph Banding Kinetics Of Chara Corallina In Alternating Light Conditions With Rectangular Pulse Voltammetry, Matthew C. Zandee

Masters Theses

Chara corallina is an important biological model due to its large internodal cells, the simple shape, and the wide range of techniques that can be used to study it. The alternating acidic and basic banding pattern of Chara corallina upon illumination has been studied and well described over the past several decades. However, much of this complex mechanism is not fully understood. Few studies have shown how the acidic and basic regions have responded in real time as lighting conditions change. Utilizing rectangular pulse voltammetry (RPV) and pH-sensitive carbon microelectrodes along the cell wall allows for a real time profile …

Scalable Parallelization Of A Markov Coalescent Genealogy Sampler, Philip E. Davis

Masters Theses

Coalescent genealogy samplers are effective tools for the study of population genetics. They are used to estimate the historical parameters of a population based upon the sampling of present-day genetic information. A popular approach employs Markov chain Monte Carlo (MCMC) methods. While effective, these methods are very computationally intensive, often taking weeks to run. Although attempts have been made to leverage parallelism in an effort to reduce runtimes, they have not resulted in scalable solutions. Due to the inherently sequential nature of MCMC methods, their performance has suffered diminishing returns when applied to large-scale computing clusters. In the interests of …

Does Phosphorus From Agricultural Tile Drains Fuel Algal Blooms?, Delilah R. Clement

Masters Theses

Phosphorus (P) is often implicated as a contributing factor to algal blooms. Attention has been focused on P in surface runoff, but agricultural tile drains also can be a source. Lake Macatawa is a hypereutrophic lake located in west Michigan, and the watershed is dominated by row crop agriculture. Further research is needed to understand the influence of bioavailable P originating from tile drains on water quality in Lake Macatawa. The objectives of this study were to 1) conduct a tile drain effluent sampling survey to assess their importance as a source of P in the Macatawa Watershed; 2) investigate …

Categorization Of Food Value Via Crayfish Aggression, Justin Thanh-Tuan Tran

Masters Theses

In numerous species, social interactions play a key role in deciding the allocation of resources. Aggression is a tactic that crayfish utilize to become dominant, which allows them to acquire higher quality resources. Many studies of aggression and agonistic interactions have used crayfish because they are known to be innately aggressive and are quick to become involved in agonistic interactions that may escalate into fighting. The primary objective of this study is to elucidate the relationship between differing food resources and their effect on aggression of crayfish. It is hypothesized that increased desirability for the food resource will induce more …

The Effect Of Seasonal Fish Migration On Energy Budgets In Two Coastal Michigan Streams, Emily M. Dean

Masters Theses

Migratory fish are known to seasonally enter coastal stream systems but the magnitude of the effects these migrations have on stream energy budgets is not fully understood. The Laurentian Great Lakes have several introduced and native adfluvial fish, where only a few studies have investigated the impacts of their migration on energy budgets in Michigan coastal streams. We quantified the contribution of energy from Chinook salmon (Oncorhynchus tshawytscha) muscle and eggs, steelhead (Oncorhynchus mykiss) eggs, and larval white suckers (Catostomus commersonii) to coastal stream energy budgets. Energy densities and energy delivered to streams were estimated using bomb calorimetry and annual …

Factors Lnfluencing Weight Gain And Perceived Barriers Of Exercise In First Semester College Students, Noah C. Neuenfeldt

Masters Theses

College students believe that university weight management interventions impact their health habits. This suggests that universities can play a large role in encouraging students to be active. The purpose of this study was to investigate this claim and to acquire a better understanding of weight change in first year college students in the first semester. We specifically sought to identify the variables that influence weight gain, as well as determine the awareness and usage of resources available to the students. A survey was given to first year students addressing the variables of interest including change in weight and Body Mass …

American Marten Denning Behavior In Michigan, Melissa J. Nichols

Masters Theses

Female American marten (Martes americana) produce litters of 1-5 kits between late March and early May. Kits remain with their mother for the duration of their first summer in a series of den structures. We located these den structures using radio-telemetry and placed remotetriggered cameras at the entrances to capture information on litter size, female activity patterns, and den visits by males and potential predators. From the summer of 2012 to the summer of 2014 we tracked 13 individual female marten (8 in the Lower Peninsula, 5 in the Upper Peninsula) with 17 litters to 75 unique den sites (60 …

Revision Of The Synechococcales (Cyanobacteria) Through Recognition Of Four Families Including Oculatellaceae Fam. Nov. And Trichocoleaceae Fam Nov. And Seven New Genera Containing 14 Species, Truc Mai

Masters Theses

A total of 48 strains of thin, filamentous cyanobacteria in the Synechococcales were studed by sequencing 16S rRNA, rpoC1, and rbcLX gene fragments. A subset of these were carefully characterized morphologically. Bayesian analysis of the 16S rRNA gene data in a large alignment of Synechococcales (345 OTU's) was in agreement with the phylogeny based on the rpoC1 gene for 59 OTU's. Both indicated that the large family-level grouping formerly classified as the Leptolyngbyaceae could be further divided into four family-level clades. Two of these family-level clades have been recognized previously as Leptolyngbyaceae and Prochlorotrichaceae. The Oculatellaceae fam. prov. and Trichocoleaceae …

Cyanomargarita Gen. Nov. (Nostocales, Cyanobacteria): Convergent Evolution Resulting In A Cryptic Genus, Sergey Shalygin

Masters Theses

Two populations of Rivularia-like cyanobacteria were isolated from ecologically diverse and biogeographically distant sites. One population was from an unpolluted stream in the Kola Peninsula of Russia, whereas the other was from a wet wall in the Grand Staircase-Escalante National Monument, a desert park-land in Utah. Though both were virtually indistinguishable from Rivularia in field and cultured material, they were both phylogenetically distant from Rivularia and the Rivulariaceae based on both 16S rRNA and rbcLX phylogenies. The new putative cryptic genus Cyanomargarita, with proposed type species C. melechinii sp. nov., and additional putative species C. calcarea are described herein. A …

Assessment Of The Efficacy Of The Hose-Bridge As A Road-Crossing Structure For Dekay's Brownsnake (Storeria Dekayi), Sarah Elizabeth Manka

Masters Theses

Various structures have been integrated into roadways to ensure the road does not impede wildlife movements between adjacent habitats. Where traffic volume is low, the costs of installing and maintaining such structures is typically prohibitive. I tested the efficacy of a hose-bridge deployed temporarily on a State Park road in Illinois that allowed the passage of cars over it and small wildlife species to cross through it. I predicted that, during the 4.5 -week period of peak migratory movement, road-based mortality of Dekay's Brownsnakes (Storeria dekayi) would be less at the site having the hose-bridge, when compared to a similar …

White-Rot Fungi As Pretreatment Agents For Wood Destined For Biofuel Applications, Ryan M. Kalinoski

Masters Theses

Efficient and economical conversion of lignocellulosic biomass to biofuels is often hampered by the chemical recalcitrance of the material. Inexpensive and environmentally-friendly pretreatment processes are therefore desirable in order to lower the cost of biofuel production and minimize negative environmental impacts. To that end, we have explored the use of a naturally occurring white-rot fungus, Trametes versicolor, as a direct pretreatment agent for hardwood destined for biofuel applications. Specifically, we examined the pretreatment effects on the hardwood mixture after treatment with the wild-type strain of T. versicolor (52J) compared with those from a cellobiose dehydrogenase (CDH)-deficient strain (m4D) , …

The Effects Of High Intensity Interval Training On Body-Esteem Among College-Aged Women, Chelsea K. Duncan

Masters Theses

The purpose of this study was to examine the effects of high intensity interval training (HIIT) on body-esteem among college-aged females. The study had four participants complete a pre-intervention questionnaire, which included BMI information and the Body-Esteem Scale (BES: Franzoi & Shields, 1984). The participants then completed 12 sessions of HIIT, three sessions each week for four weeks. After completing the HIIT protocol, participants filled out a post-intervention questionnaire, including BMI and the BES. The post-intervention questionnaires were then compared to the participants' pre-intervention questionnaires using a paired-samples t test with SPSS software. Small changes in BES scores occurred, however …

Expression Of Artemia Lea Proteins In Drosophila Melanogaster Cells Using Multicistronic Vector Constructs, Kazi Nazrul Islam

Masters Theses

In nature several organisms exhibit anhydrobiosis, the outstanding feature to survive in extreme desiccation by entering into a state of dormancy known as diapause. The cyst of the brine shrimp Artemia franciscana shows anhydrobiosis by entering into a diapause phase. Previous studies showed a correlation between anhydrobiosis and expression of highly hydrophilic polypeptides termed late embryogenesis abundant (LEA) proteins. However, the precise molecular mechanisms of LEA proteins are still unknown. The presence of multiple LEA proteins in Artemia suggests that some of them might work together. Here, I aimed to express different combination of two LEA proteins from Artemia franciscana …

Effects Of Habitat Alteration On Ecomorphology Of Fish Communities In A Restored Stream, Carl Anthony Favata

Masters Theses

Decades of anthropogenic pressure have devastated lotic ecosystems across the riverscapes of North America, resulting in degradation of critical habitat and contributing to sharp declines in biotic integrity. In response, local stream restoration projects have increased in frequency. However, little effort has been allocated to monitoring and project success has been limited. In addition, restoration projects typically focus on ecological effects above the population-level, while relationships with physiological processes are seldom assessed. Lessons from the long-term restoration and ecological monitoring of Kickapoo Creek highlight some of the complex dynamics driving reach-scale restoration projects. Following instream restoration, I predicted that alterations …

Sampling Efficiency, Population Characteristics, And Potential Impacts Of Harvest Regulations On Three Riverine Species Of Catfish, Zachary Adam Mitchell

Masters Theses

No abstract provided.

Assessing The Feasibility Of Wastewater Effluent As A Nutrient Source For Algae In The Context Of Biofuel Production, Justin Ryan Dore

Masters Theses

Algae have the potential to support the production of biodiesel due to their ability to produce large volumes of oils in relatively small footprints. However, algae production for biodiesel is hampered by the expenses associated with water and nutrients required for sufficient growth. A possible source of inexpensive and nutrient-rich water is the effluent of traditional wastewater treatment facilities. If wastewater effluent can support algae growth, the added benefit of reducing high nutrient loads to natural water systems can be realized. The overarching goal of the current research was to explore the feasibility of growing algae (Scenedesmus) using wastewater effluent …

Genetic Structure And Diversity Of Channel Catfish (Ictalurus Punctatus) In Three Large Midwestern Rivers, Vaclav Alexei Sotola

Masters Theses

Little information is available regarding the genetic structure and diversity of sportfishes in large rivers, which can be vital to continuing the sustainable exploitation of these fisheries. Additionally, impoundments often have vast impacts on riverine systems. These impacts include altering the flow regime, changing the nutrient load, and preventing migratory fish from reaching their spawning grounds. If fishes are prevented from reaching their spawning grounds, their genetic diversity and structuring could begin to change relative to historic measures, potentially decreasing their fitness and ability to survive. I screened microsatellite loci to assess the genetic population structure and diversity of channel …

Evaluation Of Hazelnuts As A Sustainable Crop In Illinois, Sharon Neva Dubosky

Masters Theses

Production of hazelnuts has historically been limited to regions with mild climates, with almost all hazelnut production in the United States occurring in Oregon. A recent boom in demand, along with an expanded selection of varieties with improved environmental tolerances, has pushed hazelnut production into new regions. Hazelnut production could prove profitable in areas of the Midwest that are less well-suited to the cultivation of maize and soybeans. However, the lack of varieties proven to thrive in the climatological conditions of this region (cold winters, hot summers, frequent drought) presents a barrier to the viability of hazelnut production. A trial …

Spatiotemporal Analysis Of Gene Flow Patterns Among Woodland Salamander Populations Inhabitating Contrasting Landscapes, Alexander C. Cameron

Masters Theses

Dispersal is a fundamental evolutionary process that serves as a mechanism by which local populations remain connected through space. Habitat loss and fragmentation remain widespread threats to biodiversity globally, and therefore it is imperative to understand how dispersal patterns are affected by anthropogenic modifications of the environment. Using a panel of 10 novel microsatellite loci, I estimated gene flow patterns over historical and contemporary timescales among populations of Eastern Red-backed Salamanders (Plethodon cinereus) in a previously unstudied portion of the species range. Four focal populations reside within a highly fragmented urban center whereas the remaining four focal populations persist in …