Life Sciences Commons^™

Phylogenetic Analysis Of Palisota (Commelinaceae) Using Chloroplast And Nuclear Regions, Alexandra H. Crum

Masters Theses

Palisota (Commelinaceae) differs from other Commelinaceae genera in androecial and pollen characters, a fleshy berry-type fruit, and anatomical characters. Palisota has been divided into two sections based on uniseriate vs. biseriate seed arrangement. Molecular phylogenetic analyses in Commelinaceae have placed Palisota near the base within the family, although its precise position is unclear. We sequenced chloroplast (matK, rbcL, rps16, and trnL-trnF, psbI-psbK, atpB-rbcL, atpF-atpH and psbA-trnH intergenic spacers) and nuclear (AT103) regions in 15 of approximately 26 species of Palisota and 15 outgroup species. Phylogenetic analyses were performed using maximum likelihood and Bayesian methods, with the …

The Association Between Dietary Niche Variation In Rodents And Climate Change Across The Paleocene-Eocene Thermal Maximum, Hannah K. Vermeer

Masters Theses

Mammalian teeth play a crucial role in food acquisition and breakdown and are therefore closely tied to dietary niche. This study reconstructed the diet of early Paleogene paramyid rodents across the Paleocene-Eocene Thermal Maximum (PETM) climatic event in an effort to understand the role of climate in mammalian dietary niche change. Dietary niches were quantified using three dental topographic measures: Dirichlet normal energy, relief index, and orientation patch count rotated. A Kruskal-Wallis test was conducted over eight time periods to determine if each of the dental topographic measures (i.e., diet) varied over time. Regression analysis of these measures with climatic …

An Assessment Of Periphyton Communities In Five Upper Peninsula Streams, Mi, Aaron Jeffrey Christiansen

Masters Theses

This project quantified lotic periphyton community change from May 2018-October 2018 in five, first and second-order Lake Superior tributary streams. Using periphyton communities, land use, geology, and abiotic factors pertinent to stream ecosystems we evaluated periphyton community succession. Using periphytometers, periphyton communities were collected and identified monthly to quantify community succession. Total phosphorus and total Kjeldahl nitrogen were measured monthly during the study. Depth, velocity, specific conductivity, and canopy cover were measured to quantify some of the physical factors within the streams. Nonmetric multidimensional scaling analysis indicated that the periphyton communities were similar between streams (ADONIS p-value =0.73) but was …

Habitat Use And Tributary Occupancy Of The Threatened River Redhorse (Moxostoma Carinatum) In The Grand River, Mi, Usa., Nicholas Michael Preville

Masters Theses

The resiliency of our aquatic ecosystems hinges on our ability to protect the native species that reside within them. The river redhorse (Moxostoma carinatum) is one such example and populations have become low enough to warrant listing by the State of Michigan. Causes of decline include overfishing, habitat alteration, and lack of knowledge of basic life-history attributes including their use of non-spawning habitat and spawning locations. In order to understand the river redhorse’s habitat use we implanted 15 individuals with radio transmitters and tracked their locations over the course of a summer. Tagged river redhorse were found to …

Dynamic Carbon Cycling In Muskegon Lake – A Great Lakes Estuary, Katie Lynn Knapp

Masters Theses

Ecosystem metabolism is the coupling of carbon and oxygen through photosynthesis and respiration. Gross primary production (GPP) is the carbon fixation by photosynthesis, ecosystem respiration (R) is carbon remineralization by bacterial and plankton respiration, and net ecosystem production (NEP) is the balance. Metabolism estimates determine if ecosystem is a sink or source of carbon to the atmosphere. When a lake has a positive NEP, or the GPP:R ratio is greater than 1, it is considered autotrophic and less carbon is being lost to the atmosphere than taken in, whereas if NEP is negative (GPP:R-1d^-1, respectively and the BOD …

Biological Invasions On A Large Scale: Investigating The Spread Of Baby’S Breath (Gypsophila Paniculata) Across North America, Sarah K. Lamar

Masters Theses

Invasive species are a serious threat to biodiversity worldwide. While the impacts of invasive species increase annually, many gaps in our understanding of how these species invade, adapt, and thrive in the novel ecosystems into which they are introduced remain. This thesis aimed to add to our knowledge of invasion science, using the perennial forb Gypsophila paniculata as a study system. Gypsophila paniculata is a shrub native to the Eurasian steppe that was introduced into North America in the late 1800’s. After introduction, G. paniculata quickly spread and now occupies diverse ecosystems across N. America. In chapter II of this …

Using Landscape Genetics To Understand Connectivity Of An Island Population Of Eastern Massasauga Rattlesnakes (Sistrurus Catenatus), Nathan Kudla

Masters Theses

Populations that experience low levels of gene flow commonly display increased levels of inbreeding, lower genetic diversity, and reduced adaptive potential. Landscape genetics allows for spatial and genetic information to be analyzed simultaneously to better understand how the landscape influences gene flow. The eastern massasauga rattlesnake (Sistrurus catenatus) is a federally threatened viper found in wetlands throughout the Great Lakes region. Many remaining populations are small and isolated due primarily to habitat loss. Atypical from a range-wide perspective, eastern massasaugas on Bois Blanc Island (BBI), Michigan live in a relatively undisturbed landscape with a potential for high connectivity …

Evaluating Remote Site Incubators To Support Restoration Of Arctic Grayling In Michigan, Alan J. Mock

Masters Theses

Remote site incubators (RSIs) have been used to rear salmonid fish eggs along streams in the Pacific Northwest since the 1980s. Recently, the successful use of RSIs for Arctic Grayling Thymallus arcticus restoration in Montana has sparked a renewed interest to reestablish the species in Michigan. To support future reintroduction efforts of Arctic Grayling in Michigan, I evaluated RSIs in three Michigan streams during 2018 and 2019 using Rainbow Trout Oncorhynchus mykiss eggs (as surrogates for Arctic Grayling). My objectives were to: (1) compare hatching success between two different RSI designs (19-L vs. 265-L RSIs), and (2) test whether the …

Postactivation Potentiation Effects From Maximal Isometric Contractions Performed At Different Hip Joint Angles, Kyle Derosia

Masters Theses

The purpose of this study was to investigate the differences in vertical ground reaction force (VGRF) production and myoelectric activity after performing maximal isometric contractions at different hip joint angles. 10 healthy, experienced participants reported to the lab on two separate occasions where they performed 3 power cleans at baseline, then 4 maximal isometric deadlifts at either ~50° hip flexion angle (Max) or ~90° hip flexion angle (Min). Following the isometric deadlifts, another 3 power clean trials at 70% 1 repetition maximum were performed and used as a post-test. The isometric deadlifts served as the conditioning contraction (CC) to induce …

Relationship Between One Repetition Maximum Parallel Squat And Jump Squat Peak Power, Corey Klitzke

Masters Theses

Jump squats are a simple exercise that can be used to train lower body power. Unlike many other methods of power training, jump squats do not require any specialized equipment (platforms, bumper plates, etc.). Jump squats can be used when other forms of power training are not available or are not allowed. In order to most efficiently train power, the intensity must balance force and velocity. The purpose of this study was to find the intensity that yields the peak power output of a jump squat. 12 participants (9 males, 3 female) participated in the study. Each participant completed a …

Thermal Quality Explains Shift In Habitat Association From Forest To Clearings For Terrestrial-Breeding Frogs Along An Elevation Gradient In Colombia, Zachary Lange

Masters Theses

Tropical ectotherms are considered particularly sensitive to changes in the thermal environment from climate change and habitat alteration. Understanding how such species’ thermal physiology relates to their habitat associations in thermally heterogeneous landscapes may help us predict responses and develop sound conservation strategies for the future. We conducted a mark-recapture study of three terrestrial breeding anuran species (Pristimantis medemi, P. savagei, P. frater) in adjacent forest and anthropogenic clearings at field sites spread across seven elevations (415-1350 m asl) in the Colombian Andes. We also performed thermal preference and critical thermal maximum assays in the lab to investigate the relationship …

Astrogliosis In The Adult Zebrafish Olfactory Bulb During Repetitive Peripheral Damage, Jackson Scheib

Masters Theses

Traumatic brain injuries (TBIs), particularly if repetitive, cause massive disruptions of brain homeostasis, significant loss of neurons, and may result in death. Astrocytes are a type of cell that maintain brain homeostasis and neuronal health. Astrogliosis is a continuum of morphological and functional changes of astrocytes in response to damage. This can be neuroprotective or neurotoxic depending on the severity and type of injury. Global astrocyte scarring is typical of neurotoxic astrogliosis and is the source of secondary injury after TBIs. The adult zebrafish olfactory system is known for its capacity to recover from trauma, however, astrocytes in this system …

Dental Topographic Analysis Of Maxillary And Mandibular Phyllostomid Bat Dentitions: Implications For Dietary Prediction In The Fossil Record, Colin Pellegrom

Masters Theses

Mammalian dental anatomy has evolved in accordance with the physical properties of its diet, and multiple features on each tooth have specific functions related to the breakdown of food during mastication and ingestion. Tooth structure is under tight genetic control and much of the anatomical variation in dentition across species is related to adaptation to a specific dietary regime. This diet-dentition relationship can be exploited to reconstruct mammalian diets from fossil specimens through calculation of dental topographic metrics. To date, most studies of dietary reconstruction using dental topography have focused on mandibular molars; thus, this study seeks to test whether …

Functional Characterization Of Glutamate Carboxypeptidase Ii In Caenorhabditis Elegans, Hashni Epa Vidana Gamage

Masters Theses

Glutamate carboxypeptidase II (GCPII) is a transmembrane zinc metalloprotease expressed in a number of organisms: from yeast to worm to humans. In humans, GCPII has been observed as a multifunctional protein and expressed in prostate, intestine, kidney, brain, tumor-associated neovasculature and other tissues as five paralogs. In the human small intestine, hGCPII is proposed to facilitate the folate absorption by cleaving terminal glutamate residues in dietary folates. Folates act as a cofactor in one-carbon metabolic pathways such as nucleotide synthesis, amino acid synthesis, DNA repair, and consequently involved in cell division and growth. The hGCPII homolog is found in the …

The Impact Of School Nutrition Education And Physical Education Programs On Weight Status Of Obese Children Aged 5-12 Years, Lamia Alotaibi

Masters Theses

Epidemiological studies indicate that 12.5 million children and adolescents are considered obese (Center for Disease Control and Prevention, 2017). Obesity is on the rise globally, and while it affects both children and adults, early measures can be taken to reduce the number of children who are obese, and therefore, come into adulthood at a higher risk for obesity-related diseases, such as hypertension, cardiovascular disease, and sleep apnea. There are many studies in which programs are implemented and tested for effectiveness; therefore, a systematic review of these studies that examine which factors play into overall obesity prevention and health improvements among …

Variation In Leaf Morphology Of An Alpine Shrub Community Along Environmental Gradients, Abdulssalam Khafsha

Masters Theses

Mountains represent complex environment gradients which strongly affect local temperature regimes and rainfall. These changes in climate conditions drive a diversity of plant responses. As alpine environments are expected to change dramatically in response to climate change, understanding plant responses to environmental variation is critical in these environments. Although they are a critical component of alpine communities, the environmental responses of shrubs have been much less examined than trees, representing an important knowledge gap. Leaves, as the primary photosynthetic organs on plants, would be expected to respond strongly to local climate conditions. In this study, I documented how the leaf …

White Grub In Bluegill In The Sangamon River: Impact Of Sewage Effluent And Flow Regime On Infection Parameters, Miranda White

Masters Theses

White grub (Posthodiplostomum minimum) is a digenetic trematode that uses a piscivorous bird, a pulmonate snail, and a centrarchid fish to complete its life cycle. To assess the impact of impoundment and sewage effluent on white grub infection parameters, infections in bluegill (Lepomis macrochirus) were monitored in 3 reaches of the Sangamon River near Decatur, Illinois: Reach 1- free-flowing river upstream of Lake Decatur Dam, unimpacted by sewage effluent, Reach 2- impounded flow below Lake Decatur Dam, unimpacted by sewage effluent, and Reach 3- free-flowing river downstream of Sanitary District of Decatur, impacted by sewage effluent. Liver and kidney abundances …

Haplotype Diversity And Population Genetic Structure Of Antistrophus Gall Wasps Associated With Two Silphium Species And Implication For Host Mediated Speciation, Jiaxin Deng

Masters Theses

Gall making insects form a special feeding guild of phytophagous animals, and by manipulating host plant tissue differentiation, are able to avoid plant chemical defenses and thus have no need for counter defense mechanisms. Host plant selection is crucial to the evolution of these insects because successful gall formation is largely dependent on host plant ability to respond to stimuli. In Illinois and neighboring states, Antistrophus gall wasps associated with the rosin weed (S. integrifolium) and the cup plant (S. perfoliatum) are morphologically indistinguishable and thus have been treated as belonging to single species. However, the wasps from the host …

Waist Circumference And The Relation To Aerobic Exercise And Perception Of Illness In Adults With Type 2 Diabetes Mellitus, Bailey J. Huebner

Masters Theses

The purpose of this study was to observe the relationship between waist circumference (WC), amount of aerobic exercise performed weekly and overall Health Belief Model (HBM) score in adults with diagnosed type 2 diabetes mellitus (T2DM). Members from a local Midwest adult fitness program (n = 5) who had been diagnosed T2DM participated in this study. Participants ranged in age from 70 to 80 years with an average age of 74 years. Four of the participants were female and one participant was male. The questions included in this study were 1 .) in those with diagnosed T2DM, was a low …

Creg1: Its Role As A Master Regulator Of Liver Function, Iffat Jahan

Masters Theses

Mammalian development involves a complex system of regulatory signals and reactions resulting in highly differentiated cell types with specific structure and function. The liver is a major organ that has been studied extensively to understand underlying genetic processes responsible for specification, establishment and maintenance of tissue identity. Hepatoma and hepatoma variant cell lines have been used as models to understand genetic networks responsible for liver function. Whole genome microarray analysis of hepatocyte cell lines has revealed candidate genes that may serve as regulators or master regulators of liver specificity. In two previous studies in our lab, the role of candidate …

Reprogramming Of Rat Hepatoma Cells With Fibroblast-Specific Protein Twist1, Kezban Ucar Cifci

Masters Theses

In mammalian development, a complex system comprised of regulatory signals causes tissue distinction with unique structures as well as functions. Gene expression controls these functions through specific combinations of transcription factors and cofactors that influence cell differentiation by both activation and repression of genes.

Whole genome microarray studies of fibroblasts have identified candidate genes that can serve as master regulators of fibroblast identity. A previous study showed that Prrx1 and Snai2 play important roles in activating expression of fibroblast identity, and Snai2 overexpression in hepatoma cells (Fg14) activated expression of fibroblast specific genes. Moreover, Snai2 overexpression resulted in repression of …

Investigating The Prevalence Of An Insecticide Resistance Gene In Culex Mosquito Populations Of Central Illinois, Kylee R. Noel

Masters Theses

The globalization of society has led to the spread of the vectors, mosquitoes, and diseases. Each year millions of people are killed by diseases vectored by mosquitoes. For example, West Nile Virus alone killed over 2,000 people in North America in 2017; 8 of those deaths being in Illinois. Two prominent vectors of West Nile Virus are Culex pipiens and Culex restuans; which are difficult to differentiate based upon morphological features. In this study, mosquitoes collected from two Illinois counties, Coles County and Champaign County, were first identified based upon morphology and this identification was then compared to probe-based …

The Correlation Between Sleep And Lifespan In Drosophila Melanogaster, Joshua Randall Lisse

Masters Theses

”Adequate sleep is associated with an individual’s health. Too little sleep is associated with many health problems, including cardiovascular disease, obesity, and a general increase in all-cause mortality. Yet the molecular changes that link poor sleep and changes in health are still not well understood. Individuals have a unique daily need for sleep, and deviations from the animal’s regular sleeping patterns can be indicative of, or result in, underlying changes in its health. Therefore, we hypothesize that changes in the sleep architecture in Drosophila melanogaster reflect changes in the fly’s health.

We determined sleep architecture in wild-type male flies over …

Characterization Of Chromosomal Translocations In A Group Of Killifish Species By Using Genome-Wide High-Density Snp Mapping Approach, Naznin Sultana Remex

Masters Theses

"The role of chromosomal rearrangements in reproductive isolation and introgression between species is poorly understood. In heterozygous form, rearrangements may directly interrupt meiotic progression leading to partial sterility/subfertility (underdominance) or may suppress local meiotic segregation (recombination suppression). Such unbalanced meiotic segregation may also result in reproductive isolation and play roles as a driving force of speciation. The objective of this study was to gain insight into the pattern of chromosomal rearrangements in two closely related killifish species in the genus Fundulus (F. notatus, and F. olivaceus) by constructing genetic linkage maps using high-resolution single nucleotide polymorphism (SNP) …

Detection Of Beta-Lactamase Variants In Municipal Wastewater And Fresh Water, Sunil Pandey

Masters Theses

The occurrence and spread of antibiotic-resistant genes (ARGs) are pressing public health problems worldwide. A key factor contributing to the spread of ARGs is lateral gene transfer. Wastewater treatment plants (WWTPs) are measured hot spots of microbial diversity and resistance because they receive polluted wastewater from diverse sources and contain a variety of different environments with dense bacterial loads. Due to the overuse of antibiotics the genetic capacities of microbes have profited. This helps every source of resistance gene and every means of horizontal gene transmission to develop the multiple mechanism of resistance to each antibiotic used clinically, agriculturally, or …

A Pipeline For Exogenous Gene Expression And Biomass Analysis In Plants, Bijay Bisunke

Masters Theses

Biological pretreatment of biomass is typically required for the production of liquid biofuels, which are viable alternatives to traditional fossil fuels. While traditional thermomechanical pretreatments are effective at helping to deconstruct lignocellulose, they are often expensive and tend to generate environmentally dangerous waste materials. An alternative to traditional pretreatment strategies is biological pretreatment, which has focused on lignocellulose-degrading organisms, such as white-rot fungi. While direct pretreatment of biomass with white-rot fungi has shown great promise, directly expressing functional white-rot fungi genes in plants (i.e. in planta expression) has the potential to be even more efficient because the biomass itself …

Medicinal Properties Of The Araliaceae, With Emphasis On Chemicals Affecting Nerve Cells, Rana Alharbi

Masters Theses

In recent times, medicinal plants have become the focus of scientists and research. However, many have used traditional medicine to take advantage of plant extracts to treat many diseases, especially neurological diseases. Various central nervous system receptors have been shown to associate with plant extracts influencing the pharmacology and in this manner conceivably assuming a role in human sickness and treatment. For instance, extracts from Cussonia paniculata Eckl. & Zeyh targeted several early nervous and mental disease, and Kalopanax pictus has been traditionally used for the treatment of rheumatoidal arthritis, nerve pain and diabetes mellitus.

In the present study, we …

Context Dependency Of Invasion Impacts On The Controllers Of Invasibility In Microstegium Vimineum, Scott Vincent Janis

Masters Theses

Although plant invasions are of major conservation concern, understanding of the natural controls on invasion and their impacts is largely limited to static observational studies or artificially manipulated systems. Linking patterns of invasion with subsequent impacts in natural systems is necessary to fully assess invasion causes and consequences. Therefore, I employed a long-term approach to sequentially assess the controls on invasion and their subsequent impacts in a self-assembled system for the highly invasive annual grass Microstegium vimineum. I modeled likely factors contributing to the probability of invasion and local invasion success of a natural invasion of M. vimineum in a …

Assessment And Recovery Of Stream Restoration Efforts On Fish Communities, Jessica Marie Rohr

Masters Theses

Aquatic systems are subjected to disturbances of various types, including natural and anthropogenic, or can deteriorate due to accumulating unfavorable conditions, including receding banks, decreasing riparian vegetation, and disrupted flow patterns. An analysis was done on a variety of streams in central Illinois that experienced one or multiple anthropogenic disturbances without remediation to determine the natural recovery dynamics of each system. A separate analysis was performed on a multi-site restored stream with a complex restoration project with a variety of restoration methods. This data were collected over at periods of time spanning from 5-15 years, including time prior to disturbance/restoration …

Impacts Of Plant-Microbe Interactions On Seedling Performance In A Riparian Forest Invaded By Lonicera Maackii, Taylor E. K. Strehl

Masters Theses

Soil microbes have profound impacts on plant growth and survival and can either promote or inhibit plant dominance. Exotic plants are often strongly invasive because they have escaped their natural enemies, potentially including antagonistic soil microbes. I examined how the invasive shrub Lonicera maackii and a common native tree, Acer negundo, responded to soil microbial communities to determine the role of soil microbes in regulating invasion success. This was done by growing both species with microbes from invaded (L. maackii) and uninvaded (A. negundo) soils collected from three locations within a riparian forest. Seedlings were …