Life Sciences Commons^™

The Effects Of 4-Nonylphenol On The Immune Response Of The Pacific Oyster, Crassostrea Gigas, Following Bacterial Infection (Vibrio Campbellii), Courtney Hart

Master's Theses

Endocrine disrupting chemicals (EDCs) are compounds that can interfere with hormone signaling pathways and are now recognized as pervasive in estuarine and marine waters. One prevalent EDC in California’s coastal waters is the xenoestrogen 4-nonylphenol (4-NP), which has been shown to impair reproduction, development, growth, and in some cases immune function of marine invertebrates. To further investigate effects of 4-NP on marine invertebrate immune function we measured total hemocyte counts (THC), relative transcript abundance of immune-relevant genes, and lysozyme activity in Pacific oysters (Crassostrea gigas) following bacterial infection. To quantify these effects we exposed oysters to dissolved phase …

The Effectiveness Of Periodically-Harvested Closures In Meeting Ecological And Socioeconomic Objectives, Paul G. Carvalho

Master's Theses

Periodically-harvested fisheries closures (PHCs) are a widespread form of community-based marine spatial management used throughout the Indo-Pacific that also is currently being intensively advocated by conservation organizations for supporting productive fisheries and healthy marine ecosystems. However, local implementation of PHCs has historically been designed to support occasional and efficient exploitation of fish stocks, and not necessarily sustainable fisheries yields and stock conservation. The efficacy of PHCs for achieving their historical cultural objectives of periodicity and efficiency of harvest, simultaneously with achieving contemporary fisheries objectives of fisheries productivity and conservation is undetermined. As a result, the utility of PHCs for supporting …

Effects Of Experimental Noise Exposure On Songbird Nesting Behaviors And Nest Success, Tracy I. Mulholland

Master's Theses

Anthropogenic noise is an increasingly prevalent global disturbance. Animals that rely on the acoustical environment, such as songbirds, are especially vulnerable to these sounds. Traffic noise, in particular, overlaps with the frequency range of songbirds, creating masking effects. We investigated the effects of chronic traffic noise on provisioning behaviors and breeding success of nesting western bluebirds (Sialia mexicana) and ash-throated flycatchers (Myiarchus cinerascens). Because anthropogenic noise exposure has the potential to interrupt parent-offspring communication and alter vigilance behaviors, we predicted that traffic noise would lead to changes in provisioning behaviors, such as fewer visits to the …

The Preservation And Protection Of Native Biodiversity In The Guadalupe Nipomo Dunes Complex, Lindsey M. Whitaker, Matthew Ritter, Scott J. Steinmaus, Jonathan Hall

Master's Theses

The Guadalupe Nipomo Dunes Complex (GNDC) is located within the California Floristic Province, a biodiversity hotspot characterized by high rates of endemism and exceptional loss of habitat. In 1980, the US Fish and Wildlife Service described the GNDC as, “the most unique and fragile ecosystem in the State of California,” and ranked it first on a list of 49 habitat areas needing state protection. It is the largest coastal dune area in California and it is one of the last remaining, relatively intact ecosystems of its type and size in the western United States. The growing recognition of species decline …

The Proteomic Response Of Gill Tissue In Tidally And Subtidally-Acclimated California Mussels, Mytilus Californianus, To Acute Emersion-Induced Anoxia, Aubrie N. Fowler, Lars Tomanek

Master's Theses

Intertidal mussels regularly experience emersion-induced anoxia, in contrast to normoxic conditions experienced during submersion. We therefore hypothesized that acclimation to a tidal rhythm, as opposed to a rhythm of constant submersion, preconditions the proteome of the California mussel, Mytilus californianus, to respond differently to emersion-induced anoxia. Following acclimation, mussels either continued to receive the acclimation conditions (control) or were exposed to 100% nitrogengas (anoxia) during aerial emersion. We collected gill tissue for subsequent analysis of protein abundance with 2D gel electrophoresis and protein identification with tandem mass spectrometry. Relative to subtidally-acclimated mussels, tidally-acclimated mussels showed a greater propensity to …

Decolonizing The Ya North: Environmental Injustice In Sherri L. Smith’S Orleans, Micah-Jade M. Coleman

Master's Theses

Young Adult (YA) dystopias, in recent years, have imagined a future world fueled by the overuse and misuse of technology, the advancement of science for human gain, as well as societies ruled by governments that govern based on their own self-interests and economic gain. Such novels have opened the door for discussion about how the present-day actions of societies can impact the future of the environment; yet many only focus their attention on societies in the North— regions considered “developed” by the western world. In her YA novel, Orleans (2014), Sherri L. Smith focuses attention on the aftermath of Hurricane …

Study Of Rehydration Properties Of Powder Produced From Chelated Skim Milk, Kristina Ellice Tan

Master's Theses

Poor rehydration properties of skim milk powder (SMP) can impact processing efficiency and functionality in finished product applications. Rehydration can be split into four stages: wettability, sinkability, dispersibility, and solubility. Previous work has suggested that chelator addition during SMP manufacture leads to higher solubility compared to SMP without chelators. This study focuses on the addition of ethylene diamine tetraacetic acid (EDTA) or sodium citrate dihydrate (SCD) at concentrations of 5, 10, and 15 mM to skim milk prior to evaporating and spray drying. The objective of this investigation was to determine the effects of the chelator additions on each SMP …

The Impact Of A Smarter Lunchrooms Makeover On Children's Food Choice And Consumption, Polina Zhuzhina

Master's Theses

Childhood obesity has tripled over the past three decades and poses a serious public health problem. The Smarter Lunchrooms Movement aims to increase healthy eating by incorporating low-cost to no-cost alterations to the school lunchroom in an effort to increase consumption of healthier foods, such as fruits and vegetables, by making them more attractive and convenient.

Our study implemented Smarter Lunchrooms interventions at two primarily Hispanic elementary schools in Paso Robles, CA. The interventions focused on increasing the appeal of the salad bars, including installation of age-appropriate signage highlighting fruits and vegetables, as well as branding of fruits and vegetables …

The Use Of Lactic Acid Bacteria To Control The Growth Of Foodborne Pathogens On Fresh-Cut Fruits And Sprout Vegetables, Franca Gabriela Rossi

Master's Theses

Growing consumer awareness of the health benefits associated with fruits and vegetables and demand for easy to prepare products has prompted the development of a wide variety of minimally processed fruits and vegetables. Minimally processed fruits and vegetables are often peeled, cut, or diced which compromise the produces’ natural protective barriers, exposing a nutrient rich medium and providing an ideal environment for the growth of microorganisms, including foodborne pathogens. The germination conditions of sprout vegetables consisting of relatively high temperatures and humidity, low light and abundance of nutrients are also conducive to the proliferation of foodborne pathogens. Recent outbreaks and …

Physical Testing Of Potential Football Helmet Design Enhancements, Michael Jeremy Schuster

Master's Theses

Football is a much loved sport in the United States. Unfortunately, it is also hard on the players and puts them at very high risk of concussion. To combat this an inventor in Santa Barbara brought a new design to Cal Poly to be tested.

The design was tested in small scale first in order to make some preliminary conclusions about the design. In order to fully test the helmet design; however, full scale testing was required. In order to carry out this testing a drop tower was built based on National Operating Committee on Standards for Athletic Equipment, NOCSAE, …

The Influence Of Exclusive Breastfeeding On Growth Trajectory From Birth To Year 5 In A Wic Cohort, Lindsey Arevalos

Master's Theses

OBJECTIVE. The impact of exclusively breastfeeding (EBF) on child body mass index (BMI) from birth through 60 months of age was investigated.

METHODS. 60,190 mothers and children attending Women, Infant and Children (WIC) clinics in the Santa Barbara and San Luis Obispo counties of California provided data on breastfeeding duration, maternal pre-pregnancy BMI, and child weight-for-length (WL) was measured at 3 month intervals until 5 years of age. Missing time points were interpolated in determining child BMI/WL z-score trajectories plotted on the Center of Disease Control (CDC) infant and child growth curves. Multivariate analysis of variance was used to contrast …

Evaluating The Myth Of Allelopathy In California Blue Gum Plantations, Kristen Marie Nelson

Master's Theses

It is widely accepted that allelopathy is not only significant, but more or less singular, in the inhibition of understory vegetation in California Eucalyptus globulus (blue gum) plantations. However, there is no published documentation of allelopathy by blue gums against California native species. Here, we present evidence that germination and early seedling growth of five California native species are not inhibited by chemical extracts of blue gum foliage, either at naturally-occurring or artificially concentrated levels. In the greenhouse, seeds were germinated in field-collected soil from mature blue gum plantations and the adjacent native, coastal scrub communities. In petri plates, seeds …

Stress Ecology Of The Pacific Rattlesnakes (Crotalus Oreganus And Crotalus Helleri), Natalie Claunch

Master's Theses

Stress is a physiological state induced by disturbance or adverse environmental conditions and is modulated by the glucocorticoid hormone corticosterone (CORT) in reptiles. Stressors can have various impacts on vertebrate trait expression and may affect survival or reproduction. Little is known about the effects of chronically elevated CORT in free-ranging reptiles, or the effect of disturbance stress on venom composition in captive snakes.

In chapter 1, we investigated the effects of researcher induced disturbance on CORT levels and venom composition in a group of captive Northern Pacific rattlesnakes (Crotalus oreganus). Venom protein concentration and plasma CORT levels were …

Investigating Novel Approaches For The Integrated Control Of The Soilborne Strawberry Pathogens Macrophomina Phaseolina And Fusarium Oxysporum F. Sp. Fragariae, Mel Carter

Master's Theses

Macrophomina phaseolina (Mp) and Fusarium oxysporum f. sp. fragariae (Fof) are emerging soilborne pathogens causing crown rot and Fusarium wilt, respectively, in commercial strawberry production in California. Fungicides representing eight active ingredients from four different mode of action groups (FRAC groups 1, 3, 7 and 12) were evaluated for their efficacy against each pathogen in vitro and each disease in planta. Fungicide active ingredients were evaluated for their ability to inhibit mycelial growth of both pathogens in vitro. Half-strength potato dextrose agar was amended with six different concentrations (0.01, 0.1, 1.0, 5.0, 10, 50 µg …

Evaluation Of The Estrogenic And Osmoregulatory Impacts Of Exposure To 4-Nonylphenol Pollution In The Estuarine Arrow Goby, Clevelandia Ios, Kaitlin Marie Johnson

Master's Theses

Recent evidence indicates that some of California’s coastal estuaries are contaminated with the chemical 4-nonylphenol (4-NP). Tissue burdens of 4-NP detected in the intertidal arrow goby (Clevelandia ios) in California are among the highest recorded worldwide, however, it remains unknown whether these fish are impacted by this 4-NP contamination. 4-NP is an established endocrine disrupting compound with estrogenic properties that can alter reproductive function. Furthermore, evidence that estrogens can modulate iono- and osmo-regulatory function in fishes implies that estuarine fishes exposed to 4-NP may also experience an impaired ability to maintain hydromineral balance. In Chapter 1 of this …

Genes Encoding Flower- And Root-Specific Functions Are More Resistant To Fractionation Than Globally Expressed Genes In Brassica Rapa, Naiyerah F. Kolkailah

Master's Theses

Like many angiosperms, Brassica rapa underwent several rounds of whole genome duplication during its evolutionary history. Brassica rapa is particularly valuable for studying genome evolution because it also experienced whole genome triplication shortly after it diverged from the common ancestor it shares with Arabidopsis thaliana about 17-20 million years ago. While many B. rapa genes appear resistant to paralog retention, close to 50% of B. rapa genes have retained multiple, paralogous loci for millions of years and appear to be multi-copy tolerant. Based on previous studies, gene function may contribute to the selective pressure driving certain genes back to singleton …

The Effects Of Quorum Sensing And Temperature On The Soluble Proteome Of Vibrio Salmonicida, Christopher L. Massey

Master's Theses

Vibrio salmonicida causes cold-water vibriosis in salmon populations around the world and causes financial damage to fisheries designed to farm these salmon. Very little is known about the physiology of how V. salmonicida causes disease and measures to contain vibriosis are restricted to either vaccinating individual fish against disease or administering antibiotics when an outbreak is detected. These procedures are costly and increase the risk for selection of antibiotic-resistant V. salmonicida strains. A recent reoccurrence of outbreaks in Norwegian fisheries provided incentive to better understand the virulence mechanisms of V. salmonicida. In this thesis, a proteomic approach was used to …

Rearing Temperature Affects The Expression Of Proteins In The Adhesive Of The Striped Acorn Barnacle, Balanus Amphitrite, Melissa J. Daugherty

Master's Theses

Barnacles are dominant hard–fouling organisms in marine waters. They attach to substrates by secreting a complex proteinaceous adhesive. Understanding the chemical composition of this multi–protein underwater adhesive and how it is affected by environmental variables, such as oceanic temperatures, is critical for developing nontoxic solutions to control biofouling. Previous experiments in our lab revealed an inverse relationship between critical removal stress (CRS) and temperatures at which barnacles were reared. Further investigations showed that this correlation is not attributed to differences in physical properties such as barnacle size or short–term changes in the viscosity of adhesive. Therefore, the observed effects may …

Comparison Of Foaming Properties Between Chelated Reconstituted Smp And Caseinates, Boya Liu

Master's Theses

Caseinate powders have been well accepted because of their foaming properties. In this study, 10% solution of reconstituted skim milk powder (SMP) chelated with sodium hexametaphosphate (SHMP) and trisodium citrate (TSC) at 1 mEq, 50 mEq, and 100 mEq were prepared to conduct a comparison with sodium caseinate, potassium caseinate, and calcium caseinate solutions. Foamability, foam stability as well as the preferential locations of αs-casein, β-casein and !-casein in their foams were analyzed. It was hypothesized that the foamability, foam stability and the preferential locations of these three caseins in the milk foams are different from treatment to treatment. Milk …

Behavioral Determinants Of Biofortified Food Acceptance: The Case Of Orange-Fleshed Sweet Potato In Ghana, Chinonso E. Etumnu

Master's Theses

Biofortified foods are being introduced in sub-Saharan Africa as an important strategy to help address micronutrient malnutrition. However, there has been little research on factors that could play decisive roles in their successful introduction. This paper investigates the determinants of consumer acceptance of biofortified orange-fleshed sweet potato (OFSP) using data from a choice experiment conducted in Ghana. I find that OFSP is preferred to traditional white-fleshed and yellow-fleshed sweet potatoes as indicated by consumers' marginal willingness to pay for the three varieties. I also find that respondents' socio-economic characteristics do not have a significant effect on consumer acceptance of OFSP. …

Separation And Characterization Of Reconstituted Skim Milk Powder Treated With Mineral Chelators, Amy Kringle

Master's Theses

The proteins found in milk are largely important in the functionality of many dairy products and dairy processes. The casein micelle system in milk is a complex and highly studied system. The micelle is thought to be a sponge like structure containing four caseins, α_s1, α_s2, β, and κ casein, and bound together with colloidal calcium phosphate. When a chelating agent such as a citrate, phosphate, or polyphosphate are added to milk systems, the CCP is bound to the chelator and removed from the micelle. It has been shown through past research that the use of …

The Effect Of Weighted Body Armor On Close Combat Reaction Time And Core Muscle Activation 2016, Christopher Banta

Master's Theses

The purpose of this study was to measure the choice reaction time and myoelectric activity of the right and left rectus abdominus, and right and left external obliques required to initiate movement in response to a visual stimulus that signaled performance of four different closecombat movements (left or right cross and left or right dodge). Reaction time and myoelectric activity were then compared with performing the movements in response to the visual stimulus while wearing a weighted vest that simulated wearing tactical body armor. Myoelectric activity was measured as the average root mean square (RMS) of the surface electromyography (sEMG) …

Power Output And Loads On The Lumbar Vertebrae During A Power Clean 2016, Joseph Keleher

Master's Theses

The purpose of the present study was to compare the peak resultant torque, shear, and compressive forces acting at the L3/L4 junction of the lumbar vertebrae and the peak power output during 40% one repetition maximum (1RM) and 80% 1RM attempts of the power clean. It was hypothesized that performance of a power clean at 80% 1RM will result in greater peak resultant compressive force, torque and shear force at the L3/L4 junction of the lumbar spine than that of a 40% 1RM attempt. Power cleans at 80% 1RM will also result in higher maximum instantaneous power outputs than that …

Do Running Kinematics Change On The Alter-G Treadmill? [2016], Brittany Lavaute

Master's Theses

The Alter-G lower body positive pressure treadmill, also known as the antigravity treadmill, provides a unique means of exercising for rehabilitation patients and low-impact training in athletes. The purpose of this study was to examine the interaction effect of four different treadmill weighted conditions and three different running velocities (2.68 m.s-1, 3.13 m.s-1, and 3.58 m.s-1) on the kinematic variables of step length, step rate, contact time, and flight time on the Alter-G treadmill (AG) and on the regular treadmill (TM). Fifteen participants completed two separate days of testing. All individuals ran at four different conditions (100%, 80%, 60%, and …

Determining The Optimal Combination Of Lifting Method And Intensity For Power Production During The Hang Clean 2016, Thomas P. Murray

Master's Theses

The purpose of this study was to determine the optimal combination of lifting method and load intensity for power production during the hang clean. Twenty college students (18 male, 2 female) performed one set of three repetitions of the hang clean at four different intensities for each method while standing on a force platform embedded in the floor. Intensities were set at 50%, 60%, 70%, and 80% 1RM and the methods tested were Contact, where the participant maintained contact with the force platform throughout the lift, and Jump, where the participant jumped through the pull phase of the lift and …

The Effects Of Whole Body High-Intensity Interval Training On Collegiate Female Soccer Players 2016, Whitney Frary

Master's Theses

Soccer is considered a high intensity sport that requires a high level of both aerobic and anaerobic fitness. A whole body high-intensity interval training adaptation has been shown to benefit both of these systems over a short period of time. The purpose of this study was to examine the effects of a five week whole-body high intensity interval training program on female collegiate soccer players performance in speed, acceleration, agility, vertical jump, aerobic capacity, physical self perception, and physical enjoyment. Participants were from the SUNY Cortland women’s soccer team who participated in a five- week training protocol three days a …

The Effect Of Descriptive Norms On Resistance Exercise Self-Efficacy In College-Aged Females 2016, Justin Kompf

Master's Theses

Resistance training is a form of physical activity that provides substantial health benefits. Despite these widespread benefits, participation in resistance training is considerably low, particularly among females. To engage in a skill-related activity such as resistance training, individuals need to have confidence in their abilities. Self-efficacy is a cognitive construct that is used to describe situation-specific self-confidence. Descriptive norms are a type of social norm that describes the behavior of others. Descriptive norms have been useful in positively changing health related behaviors. The exact mechanism of how descriptive norms alter behavior is unknown. However, it has been show in research …

Fire And Fuels: Vegetation Change Over Time In The Zuni Mountains, New Mexico, Luke Wylie

Master's Theses

The Zuni Mountains are a region that has been dramatically changed by human interference. Anthropogenically, fire suppression practices have allowed a buildup of fuels and caused a change in the fire-adapted ponderosa pine ecosystem such that the new ecosystem now incorporates many fire-intolerant species. As a result, the low-severity fires that the ecosystem once depended on to regenerate the forest are much reduced, and these low-severity fires are now replaced by crown-level infernos that threaten the forest and nearby towns. In order to combat these effects, land managers are implementing fuel reduction practices and are striving to better understand the …

Fishery And Population Dynamics Of Mississippi’S Spotted Seatrout, David Arthur Dippold

Master's Theses

Spotted Seatrout (Cynoscion nebulosus) is the most popular recreational inshore fishery in Mississippi coastal waters. Because of the popularity of Spotted Seatrout in Mississippi as a recreational target and the magnitude of harvest, quantitative approaches to describe the population and fishery dynamics of the Mississippi stock are needed to continue the sustainability of the stock. Quantitative approaches to describing stock dynamics are useful because they can help describe population characteristics, assess the current status of the stock, and be used to evaluate alternative management strategies. In this thesis, I use a suite of quantitative methods to describe, evaluate, …

Effects Of Intermittent Pneumatic Compression On Leg Blood Flow And Vascular Function After Spinal Cord Injury, Lena Marie Cialdella

Master's Theses

Intermittent pneumatic compression (IPC) can increase leg blood flow (BF) in able-bodied persons. Whether IPC can alter leg BF, and improve vascular function in people with SCI is currently unknown. PURPOSE: Test the hypothesis that acute IPC will increase leg BF, and improve vascular function in SCI. METHODS: Participants (n=8; injury level: T3 and below; A.S.I.A. class A-C; age: 41±17 yrs) were recruited for a 1-hour IPC session performed in one leg (experimental leg; EXP), with the other serving as a control (CON). IPC consisted of sequential, foot-to-calf compressions (4-s inflate, 16-s deflate; 3 compressions/min). Posterior-tibial artery BF (Doppler-ultrasound) was …