Life Sciences Commons^™

Pink And Dude Chefs: Efficacy Of An Online Train-The-Trainer Mechanism And Student Program Outcomes, Jacqueline Chen

Master's Theses

Background: The rate of overweight and obesity among adolescents aged 12-19 years has quadrupled since 1980. Reducing obesity is a key public health priority, as obesity is associated with individual and population-level health and economic consequences. Afterschool-based obesity prevention programs that aim to develop nutrition and culinary self-efficacy have shown promise. However, the level of aptitude among program instructors is directly correlated with student success, suggesting the importance of effective train-the-trainer (TTT) mechanisms for implementing and scaling up these strategies.

Pink and Dude Chefs (PDC) is an afterschool nutrition education and culinary skills program for middle-school adolescents aged between 11-14 …

The Recovery Of Protein From Egg Yolk Protein Extraction Granule Byproduct, Irene Jennifer Kaufman

Master's Theses

In addition to proving an excellent source of nutrients, eggs are used in the food, cosmetic, and biotechnology industries for their rheological and bioactive properties. Much of the potential for the added value is in individual components of the egg, rather than the whole egg. At low speed centrifugation, yolk separates into two distinct fractions—granules and plasma. It is becoming increasingly popular in the industry to remove the plasma fraction of the egg yolk to use for its livetins, particularly immunoglobulin Y, leaving behind a granule by-product (“yellow cake”). Previous research has shown potential added-value from the granule fraction, especially …

Effects Of Food Consumption On Cell Proliferation In The Brain Of Python Regius, Stacy Star Habroun

Master's Theses

Neurogenesis is an important and vastly under-explored area in reptiles. While the ability to generate new brain cells in the adult mammalian brain is limited, reptiles are able to regenerate large populations of neuronal cells. Pythons exhibit a characteristic specific dynamic action (SDA) response after food intake with an increase in metabolic rate that facilitates processing the meal. Associated with this change in SDA, pythons (Python spp.) also exhibit impressive plasticity in their digestive and cardiovascular physiology due to the sheer magnitude of the increase in organ growth that occurs after a meal to speed digestion, absorption, and assimilation of …

The Population Genetics Of Morro Bay Eelgrass (Zostera Marina), Julia Gardner Harencar

Master's Theses

Seagrass populations are in decline worldwide. Zostera marina (eelgrass), one of California’s native seagrasses, is no exception to this trend. In the last 8 years, Morro Bay, California has lost 95% of its eelgrass. Eelgrass is an ecosystem engineer, providing important ecosystem services such as sediment stabilization, nutrient cycling, and nursery habitats for fish. The failure of recent restoration efforts necessitates a better understanding of the causes of eelgrass decline in this estuary. Previous research on eelgrass in California has demonstrated a link between population genetic diversity and eelgrass bed health, ecosystem functioning, and resilience to disturbance and extreme climatic …

Effects Of Ankle Weights On Metabolic Response And Muscle Activity On A Lower Body Positive Pressure Treadmill 2017, Saige Hupman

Master's Theses

Lower body positive pressure (LBPP) treadmills are growing in popularity for rehabilitative use, as the benefits of exercising at partially supported body weight may induce faster recovery. It is unknown if there are certain practices that increase exercise intensity while maintaining positive effects of LBPP. Adding ankle weights when walking or running could increase intensity of rehabilitation programs while maintaining the comfort of supported body weight. PURPOSE: To measure metabolic response (VO2, RER, HR, Caloric expenditure), RPE, and lower limb electromyography (EMG) amplitudes of LBPP treadmill walking and running with and without ankle weights. METHODS: Sixteen participants (Age: 21.94 ± …

The Effects Of Quadmill Training On Balance: An Intervention Study 2017, Alexander J. Generali

Master's Theses

The purpose of this study was to observe and compare the effects of two separate training interventions using the QuadmillTM to a control group on balance in college-aged individuals. It was hypothesized that both experimental groups (I1 and I2) would experience improvements in balance over the intervention whereas the control group would remain at baseline. It was secondarily hypothesized that I2 would experience greater improvements than I1 in balance due to the nature of the exercise protocol. Data was collected using three systems; Balance Tracking Systems, Star Excursion Balance Test, and the Biodex Balance system. A two-way mixed methods ANOVA …

The Effect Of Blood Flow Restriction Training With The Quadmill On Peak Isometric Knee Extensor Strength 2017, Brandon Schrom

Master's Theses

Traditional training methodologies that improve muscular strength use loads as low as 75% of a person’s one-repetition maximum and as high as 110% of a person’s one repetition maximum. With these high loads comes a greater risk for injury. Blood flow restriction (BFR) training is a potential solution to this problem. BFR training originated in Japan, where it was called Kaatsu. With this method of training, a trainee ties a tourniquet around the proximal end of a limb to reduce blood flow to and from the limb’s muscles. The purpose of this study was to determine whether three-weeks of BFR …

Imposed Versus Affect-Based Resistance Training Intensities On Adherence, Session Affect, Session Perceived Exertion, And Intrinsic Motivation Measures During A Six-Week Program In Novice Female Lifters 2017, Ryan Joseph Brennan

Master's Theses

The American College of Sports Medicine (ACSM) recommends that individuals perform resistance training exercises at a specific intensity based on external load (percentage of one repetition maximum; %1RM). However, only 29.6% of adults in 2013 reported strength training two or more times per week. Furthermore, individuals lifting at recommended percentages vary in their pleasure and displeasure. Self-selected exercise seems to promote positive affective responses, in part, due to the perceived autonomy. The effects of regulating exercise intensity using affect as opposed to imposed intensities as a means for improved fitness, promoting exercise behavior, and enhancing other psychological outcomes have yet …

The Relationship Between Ratings Of Perceived Exertion And Heart Rate In Ncaa Division Iii Male Soccer Players 2017, Samuel J. Guider

Master's Theses

Every sport has specific physical demands of the human body. The amount of physiological load that an athlete endures during a bout of exercise can be described as training load (TL). Accurate calculation of training loads within athletes is important when it comes to strength and power development, as well as injury prevention and monitoring fatigue. Common methods used to calculate training loads for athletes include rating of perceived exertion (RPE) based methods, heart rate (HR) based methods, rate of oxygen consumption (VO2) methods, and blood lactate methods. Specifically with NCAA male soccer athletes, HR based methods and RPE based …

Does Personality Similarity In Bottlenose Dolphin Pairs Influence Dyadic Bond Characteristics?, Kelsey R. Moreno

Master's Theses

Social structures are critical to the success of many species and have repercussions on health, well-being, and adaptation, yet little is known about the factors which shape these structures aside from ecology and life history strategies. Dyadic bonds are the basis of all social structures; however, mechanisms for formations of specific bonds or patterns in which individuals form which types of bonds have yet to be demonstrated. There is a variety of evidence indicating personality may be a factor in shaping bonds, but this relationship has not been explored with respect to bond components and is yet to be demonstrated …

Do Pinnipeds Have Personality? Coding Harbor Seal (Phoca Vitulina) And California Sea Lion (Zalophus Californianus) Behavior Across Contexts, Amber J. De Vere

Master's Theses

Personality has now been studied in species as diverse as chimpanzees (King & Figueredo, 1997) and cuttlefish (Carere et al., 2015), but marine mammals remain vastly underrepresented in this area. A broad range of traits have been assessed only in the bottlenose dolphin (Highfilll & Kuczaj, 2007), while consistent individual differences in a few specific behaviors have been identified in grey seals (Robinson et al., 2015; Twiss & Franklin, 2010; Twiss, Culloch & Pomeroy, 2011; Twiss, Cairns, Culloch, Richards & Pomeroy, 2012). Furthermore, the context component of definitions of personality is not often assessed, despite evidence that animals may show …

Sparking A Dolphin's Curiosity: Individual Differences In Dolphins' Reactions To Surprising And Expectation-Violating Events, Malin Katarina Lilley

Master's Theses

Non-scientific literature consistently describes dolphins as “curious animals,” but there has been little systematic research on curiosity in dolphins. Curiosity in humans and certain non-human animal species, including birds and non-human primates, has been studied by examining individual differences in exploration and reactions to novel stimuli. Additionally, research has explored how human infants and non-human animals react when an event violates their expectations. The present study explored dolphins’ reactions to spontaneously surprising and expectation-violating stimuli. The reactions of dolphins, 15 bottlenose (Tursiops truncatus) and 6 rough-toothed (Steno bredanensis), at Gulf World Marine Park were analyzed in …

Life History Of The Non-Native Invasive Red Lionfish (Pterois Volitans) In The Northern Gulf Of Mexico, Alexander Q. Fogg

Master's Theses

Invasive Red Lionfish (Pterois volitans) were first detected in the northern Gulf of Mexico (nGOM) in 2010 and since then their numbers have increased dramatically. From 2010 to 2015, more than 15,000 Red Lionfish were collected opportunistically from the nGOM for this study. Length and weight relationships differed significantly among ecoregions by sex and there was clear sexual dimorphism in size with males being larger and heavier. Red Lionfish age ranged from 0-4.5 years old and males achieved greater growth rate (K) and asymptotic maximum lengths (L_inf) compared to females and these parameters were also different …

Age, Growth, And Reproduction Of Vermilion Snapper (Rhomboplites Aurorubens) In The North-Central Gulf Of Mexico, Trevor Dalton Moncrief

Master's Theses

Vermilion Snapper is a commonly harvested species of reef fish in the northern Gulf of Mexico (GOM). It supports both a large commercial and popular recreational fishery, however knowledge of this fish’s life history is limited spatially. Non-linear curve fitting was used to estimate growth parameters and Akaike information criteria (AIC) was used to determine relative model fit. The 2-parameter von Bertalanffy growth function provided the best model fit and lowest AIC score. Histological examination indicated that Vermilion Snapper are batch spawners with asynchronous oocyte development. Additionally, 17% of Vermilion Snapper in the actively spawning phase containing 24 hour POF’s …

The Effect Of Boat Type On Bottlenose Dolphin (Tursiops Trucatus) Behavior In The Mississippi Sound, Maria Zapetis

Master's Theses

Increases in oceanic shipping are a global phenomenon, and a leading cause of concern for marine animal welfare. While it may be difficult to assess the effect of boat traffic on all species in all contexts, it is vital to report anthropogenic impacts where longitudinal data is available, and doubly so where a dearth of information exists. The purpose of this study is to describe how dolphin behavior changed in the presence of boats in the Mississippi Sound between 2006 and 2012, and more specifically, to detail how different boat types impacted dolphins’ behavioral states. This study is unique in …

Characterization Of Andrenocortical Tissue Morphology, Histology, And Steroid Synthesis Among Finetooth (Carcharhinus Isodon), Blacktip (Carcharhinus Limbatus), Atlantic Sharpnose (Rhizoprionodon Terraenovae), And Bonnethead (Sphyrna Tiburo) Sharks, Danielle Elizabeth Bailey

Master's Theses

Adrenocortical (interrenal) tissue is composed of steroidogenic cells that produce corticosteroids involved in the stress response and hydromineral balance. Previous research characterizing the elasmobranch interrenal suggests that the number of interrenal bodies that produce the single primary corticosteroid, 1a-hydroxycorticosterone (1a-OHB), varies among species. However, potential species-specific differences in the amount of interrenal tissue and major steroid products produced have been understudied. To address this critical gap in our understanding of elasmobranch biology, this study examined interrenal morphology, cell structure, steroidogenic enzyme distribution (3bHSD), and steroid production in four shark species: Finetooth Carcharhinus isodon, Blacktip Carcharhinus limbatus, Atlantic Sharpnose …

The Insulin-Like Growth Factor-1 (Igf1) System As A Potential Biomarker For Nutritional Status And Growth Rate In Pacific Rockfish (Sebastes Spp.), Nicole L. Hack

Master's Theses

Growth performance in vertebrates is regulated by environmental factors including the quality and quantity of food, which influences growth via endocrine pathways such as the growth hormone (GH) / insulin-like growth factor somatotropic axis. In several teleost fishes, circulating concentrations of insulin-like growth factor-1 (Igf1) correlate positively with growth rate, and it has been proposed that plasma Igf1 levels may serve as an indicator of growth variation for fisheries and aquaculture applications. Here, I tested whether plasma Igf1 concentrations might serve as an indicator of somatic growth in olive rockfish (Sebastes serranoides), one species among dozens of rockfishes …

An Assessment Of Potential False Positive E.Coli Pyroprints In The Cplop Database, Skyler A. Gordon

Master's Theses

The genetic information found in each species of organism is unique, and can be used as a tool to differentiate at the molecular level. This has caused rapid genotyping methods to become the cornerstone of a new area of research dependent on reading the genome as a form of identification. One of these specific identification methods, known as pyroprinting, relies on the small variation of DNA sequences within the same species to develop a unique, reproducible fingerprint. By simultaneously pyrosequencing multiple polymorphic loci within the ribosomal operons known as the intergenic transcribed spacers, a reproducible output is obtained, known as …

Plant Compound Pest Control In California Strawberry (Fragaria × Ananassa) Production, Eli Mahanes Weissman

Master's Theses

Allelopathy occurs when one organism releases a compound into the environment that affects the functioning of another organism. Scientists have long suspected that alleopathic plant compounds could offer novel, softer chemistries to the ongoing battle of controlling pests in agricultural fields. Strawberry growers rely on toxic fumigants to kill soilborne fungal pests, weeds, nematodes, and insects. Increased regulations have reduced the use of fumigants (including methyl bromide), and strawberry growers need new sustainable pest control solutions. We selected four putative allelochemicals with known fungicidal and herbicidal activity (ferulic acid, gallic acid, juglone, and p-Coumaric acid). We assessed the pesticidal activity …

The Validity Of Height As A Determinant Of Takeoff Distance To Hurdle One In Ncaa Division Iii Hurdlers 2017, Phillip Wiltshire

Master's Theses

The purpose of this study was to determine if the relationships between the height of a hurdler and the takeoff distance to the first hurdle for men and women as reported by Frye (2008) were valid for NCAA Division III 100 m and 110 m hurdlers. With no specific research reported to validate Frye’s data, it is unclear how the suggested takeoff distances were developed. It is also unclear whether hurdler height or another factor is the main determinant of takeoff distance for the high hurdlers. Participants in this study were three female 100 m hurdlers and four male 110 …

Araucaria In The Urban Landscape: A Novel Leaning Pattern And Evidence Of Cultivated Hybridization, Jason W. Johns

Master's Theses

Our understanding of the natural world is constantly evolving and strengthening as more observations are made and experiments are performed. For example, we understand that tree stems grow toward the light (positive phototropism; Darwin 1880, Loehle 1986, Christie et al. 2013) and against gravity (negative gravitropism; Knight 1806, Hashiguchi et al. 2013). We also know that plants respond to mechanical stimulus and perturbation (thigmotropism; Braam 2005). Genes and their resulting proteins have been described to uncover some of the mechanisms for these environmental responses, but relatively speaking, we have just scratched the surface (Wyatt et al. 2013). While the discovery …

Defining The Roles Of The Lipoic Acid Ligases In Promoting Staphylococcus Aureus Metabolic Homeostasis And Virulence, Irina Laczkovich

Master's Theses

Staphylococcus aureus is a major human pathogen known to cause disease in a wide range of tissues. In order to thrive in such diverse environments, S. aureus uses multiple adaptive traits such as trace metal/nutrient acquisition, shifts in metabolic activity, and expression of detoxification systems, all of which allow the bacterium to proliferate and survive in nutritionally deficient and inhospitable environments.

One essential metabolite used by S. aureus is lipoic acid, a cofactor of enzyme complexes used in aerobic metabolism, fatty acid biosynthesis, glycine detoxification, and maintenance of redox homeostasis. Prior studies in the lab used a genetic approach to …

Utilizing Single-Molecule Fret Methods To Study Conformational Changes In Trim5Α, Margret Suzanne Bradley

Master's Theses

Single-molecule FRET (smFRET) is a method by which dynamic conformational changes can be monitored in a protein microscopically and in real time. smFRET relies on the creation of FRET (Förster Resonance Energy Transfer) between small molecule fluorophores conjugated to the biomolecules of interest. FRET efficiency allows calculation of interfluorophore distances. Changes in FRET efficiency represent changes in protein conformation which can inform further structural and molecular studies of the protein of interest. For example, in the Campbell Lab, we study the protein TRIM5α, an antiretroviral cellular protein which can cause premature dissociation of the HIV capsid core by an unknown …

Potential Efficacy Of Targeting Mll1 In Breast Cancer, Austin Gable Holmes

Master's Theses

In 2017, it is estimated that breast cancer will be the most prevalent newly diagnosed cancer in females, at 30% in the U.S. alone1. From the early 1990s to 2014 the death rate in females from breast cancer has dropped by 10% but still stands as the second highest cancer related death in females in the U.S.2. One of the biggest hurdles in breast cancer research is disease heterogeneity. New mechanisms of disease development and progression are encountered frequently. One mechanism studied in the past decades is epigenetics. It has been theorized that the cancer epigenome can maintain an abnormal …

The Novel Characterization Of Extracellular Vesicles Containing Proteins Which Have Been Implicated In The Pathogenesis Of Hiv Associated Neurocognitive Disorders, Grace Ispas

Master's Theses

Although Anti-Retroviral Therapy (ART) is practiced, HIV-1 positive individuals still experience HIV-Associated Neurocognitive Disorders (HAND), collectively described as the presentation of neurocognitive, behavioral and motor dysfunctions that decrease the quality of life, while increasing the mortality in ART suppressed HIV-1 positive patients.

Current literature suggests that extracellular vesicles are involved in the pathogenesis of HAND as they are believed to be transferring HIV-1 proteins to nearby neuronal cells. Although most studies assume homogeneity among populations, characterizing cellular proteins or RNA levels in bulk, we hypothesize that distinct populations of extracellular vesicles are released and that environmental conditions, including viral infections, …

Functional Studies Of Novel Mosquito Stage-Specific Genes In The Malaria Parasite, Plasmodium Berghei., Kaitlyn Kiernan

Master's Theses

The development and survival of the malaria parasite Plasmodium within its mosquito vector is not very well understood. When the parasite is taken up by an Anopheles mosquito, it is exposed to digestive enzymes, reactive oxygen species, increasing pH, decreasing temperature, and a diverse microbial flora. Our lab is studying the adaptive mechanisms underlying parasite survival in this hostile environment, one of which is movement out of the midgut. In order to escape the midgut, the parasite develops into an elongated, motile form called the ookinete. I am investigating two genes, thioredoxin-like protein 1 (trxl-1) and subpellicular microtubule protein 1 …

Phenotyping Temperature-Sensitive Coronaviruses, Amani Eddins

Master's Theses

Coronaviruses (CoVs) can cause a range of symptoms; from a mild common cold to life threatening acute respiratory distress syndrome (ARDS) upon infection. These emerging viruses have the ability to be highly pathogenic and detrimental to the human population. Two prime examples of CoV emergence, SARS-CoV (Severe Acute Respiratory Syndrome) and MERS-CoV, (Middle Eastern Respiratory Syndrome), which exhibits the pandemic potential of emerging CoVs that gain human tropism. Given the increasing potential of an emerging CoV outbreak there is a state of urgency to develop vaccines that will help protect the human population against current and future circulating strains. The …

The Effects Of Treating Multispecies Biofilms With Bacteriophage, Alexandria Francis Cooper

Master's Theses

Biofilms can be found in any environment within proximity to water and are problematic in an assortment of industries. Numerous efforts have been employed to dislodge biofilms including bacteriophages (viruses that infect bacteria). Phage remediation is a promising solution for combatting biofilms that form on catheters in long term use patients. These biofilms often result in catheter-associated urinary tract infections (CAUTIs) and are the most common type of healthcare related infection reported. Additionally, they result in longer hospital stays and increased morbidity. To date, most of the research on the topic focuses on single species biofilms, despite their rarity in …

Utilizing Genetic Techniques To Identify Amino Acids Within The Putative Glycosyltransferase Sypq That Are Essential For Its Role In Biofilm Formation By Vibrio Fischeri, Mary Kathryn Flaherty

Master's Theses

The polysaccharide component of the V. fischeri biofilm is produced by proteins encoded by the symbiosis polysaccharide (syp) locus. The syp locus encodes 18 genes, six of which are putative glycosyltransferases (GTs). One of these GTs, sypQ, encodes a putative family 2 glycosyltransferase (GT-2). The mechanism by which SypQ facilitates biofilm formation is not yet understood. To uncover which specific residues are essential for the function of SypQ, I utilized Bioinformatics, site-directed mutagenesis, and random mutagenesis. The Bioinformatics identified putative GT-2 motifs in the SypQ primary sequence. The mutagenesis (both site-directed and random), identified five residues (D95, D151, S153, D236, …

Optimizing Clean Catch Urine Collection And Its Applications In Urinary Microbiome Studies, Danielle Johansen

Master's Theses

Clean catch was developed before the advancement of more sensitive assays for culturing urinary bacteria. I reassessed clean catch, endeavoring to optimize this method. Periurethral swabs and voided urines were routinely collected, while analyzing different aspects of the method. I determined that midstream urine contained the least amount of vulvo-vaginal contamination and that the time of collection did not impact the results. I also determined that the use of antiseptic wipes prior to void increased the level of vulvo-vaginal contamination. A small cohort of females collected daily urines over a 17-day period and kept a strict alcohol diary. Females who …