Life Sciences Commons^™

The Application Of An Implementation Science Framework To Comprehensive School Physical Activity Programs: Be A Champion!, Justin B. Moore, Russell L. Carson, Collin A. Webster, Camelia Renee Singletary, Darla M. Castelli, Russell R. Pate, Michael W. Beets, Aaron Beighle

Faculty Publications

Comprehensive school physical activity programs (CSPAPs) have been endorsed as a promising strategy to increase youth physical activity (PA) in school settings. A CSPAP is a five-component approach, which includes opportunities before, during, and after school for PA. Extensive resources are available to public health practitioners and school officials regarding what should be implemented, but little guidance and few resources are available regarding how to effectively implement a CSPAP. Implementation science provides a number of conceptual frameworks that can guide implementation of a CSPAP, but few published studies have employed an implementation science framework to a CSPAP. Therefore, we developed …

Exosomes Serve As Novel Modes Of Tick-Borne Flavivirus Transmission From Arthropod To Human Cells And Facilitates Dissemination Of Viral Rna And Proteins To The Vertebrate Neuronal Cells, Wenshuo Zhou, Michael Woodson, Biswas Neupane, Fengwei Bai, Michael B. Sherman, Kyung H. Choi, Girish Neelakanta, Hameeda Sultana

Faculty Publications

Molecular determinants and mechanisms of arthropod-borne flavivirus transmission to the vertebrate host are poorly understood. In this study, we show for the first time that a cell line from medically important arthropods, such as ticks, secretes extracellular vesicles (EVs) including exosomes that mediate transmission of flavivirus RNA and proteins to the human cells. Our study shows that tick-borne Langat virus (LGTV), a model pathogen closely related to tick-borne encephalitis virus (TBEV), profusely uses arthropod exosomes for transmission of viral RNA and proteins to the human- skin keratinocytes and blood endothelial cells. Cryo-electron microscopy showed the presence of purified arthropod/neuronal exosomes …

Game Spirituality: How Games Tell Us More Than We Might Think, Chad Carlson

Faculty Publications

While we often see games as less serious or at least less transcendental than religion there is reason to believe that games can evoke similarly meaningful narratives that allow us to learn a great deal about ourselves and our world. And games do so often using the same symbolic and metaphorical mechanisms that generate meaning in religious experience. In this paper, I explore some of the ways in which game myths—the myths created from and through games—generate meaning in our lives. People experience myths in games very similarly to how they might in religion. I first explain what myth means …

Radial Growth Responses Of Upland Oaks Following Recurrent Restoration Treatments In Northern Mississippi, Kathryn R. Kidd, J. Morgan Varner, J. Stephen Brewer

Faculty Publications

Fire exclusion over the past century has substantially altered composition, structure, and fuel dynamics in upland oak-hickory (Quercus-Carya) forests in the Southeastern United States. Numerous restoration efforts have been made to re-establish historical disturbance regimes into these altered forests. However, our understanding of the implications of restorative disturbances on stand dynamics has primarily been limited to shifts in species composition and post-disturbance regeneration. Therefore, we examined annual radial growth responses of dominant upland oaks following a combination of prescribed fires (2004, 2006, 2008, 2010, 2012, and 2014) and thinning (starting in 2004) treatments (thin+burn) in stands which had previously been …

Use Of Video Feedback In The Training Of Pre-Service Teachers, Amanda Andrews, Linda Bobo, Amy Spurlock

Faculty Publications

The purpose of this study was to use video feedback in the training of pre-service clinical educators. Thirty athletic training teacher participants were randomly placed in one of three equal groups where they were provided with the following interventions: a) teaching seminar and opportunity to observe video of teaching; b) teaching seminar; and c) control group. These groups were videotaped for eight consecutive weeks while teaching clinical skills. The researchers analyzed the feedback provided by the teachers using a modified version of the Fishman’s Augmented Feedback Instrument. Allowing teachers the opportunity to view their videotapes with guided feedback showed that …

Teens-As-Teachers Nutrition Program Increases Interest In Science Among Schoolchildren And Fosters Self-Efficacy In Teens, Virginia Bolshakova, John Gieng, C. Sheena Sidhu

Faculty Publications

The Healthy Living Ambassador Program brings health, teen leadership, and teamwork to California's elementary school gardens through interdisciplinary UC Cooperative Extension collaboration, community-based partnerships and teen teaching. During spring 2015, teen ambassadors trained by Extension educators and volunteers at UC Elkus Ranch in San Mateo County taught nutrition science, food cultivation and healthy living skills in an 8-week, garden-based, after-school nutrition and physical education program for elementary school children in an urban setting. We conducted a pilot study using a mixed-methods approach to measure and explore the program's impact on children's vegetable selection and consumption preferences, as well as perceived …

Teens As Teachers In The Garden: Cultivating A Sustainable Model For Teaching Healthy Living, Virginia Bolshakova, John Gieng, C. Sheena Sidhu, Mary Vollinger, Lorena Gimeno, Jessica Guild

Faculty Publications

School gardens are an ideal space to deliver a healthy living curriculum, such as nutrition and physical activity education, to elementary school youth. However, public schools often lack the resources and support to establish sustainable garden-based programming. We created the Healthy Living Ambassador program, a collaborative after-school garden program in low-income communities that brought together resources from schools, community programs, and University of California Cooperative Extension. This school garden program featured culturally competent teens as teachers to serve as near-peer educators and mentors to elementary school youth. The program development model incorporated lessons from sustainable community-based health program interventions and …

Incidence Of Second Malignancy In Patients With Papillary Thyroid Cancer From Surveillance, Epidemiology, And End Results 13 Dataset, Mayumi Endo, Jessica Liu, Marcelle Dougan, Jennifer Lee

Faculty Publications

Increased risk of second primary malignancy (SPM) in papillary thyroid cancer (PTC) has been reported. Here, we present the most updated incidence rates of second primary malignancy from original diagnosis of PTC by using the data from the Surveillance, Epidemiology, and End Results. In this cohort, 3,200 patients developed SPM, a substantially higher number than in the reference population of 2,749 with observed to expected ratio (O/E) of 1.16 (95% CI; 1.12–1.21). Bone and joint cancer had the highest O/E ratio of 4.26 (95% confidence interval [CI] 2.33–7.15) followed by salivary gland (O/E 4.15; 95% CI 2.76–6.0) and acute lymphocytic …

The Roles Of Inter- And Intra-Sexual Selection In Behavioral Isolation Between Native And Invasive Pupfishes, Cory Becher, Jennifer M. Gumm

Faculty Publications

Male-male competition and female mate choice may both play important roles in driving and maintaining reproductive isolation between species. When previously allopatric species come into secondary contact with each other due to introductions, they provide an opportunity to evaluate the identity and strength of reproductive isolating mechanisms. If reproductive isolation is not maintained, hybridization may occur. We examined how reproductive isolating mechanisms mediate hybridization between endemic populations of the Red River pupfish Cyprinodon rubrofluviatilis and the recently introduced sheepshead minnow C. variegatus. In lab-based dominance trials, males of both species won the same number of competitions. However, male C. rubrofluviatilis …

The Effects Of Ultraviolet Light On Anthocyanin Accumulation In The Adventitious Roots Of Sedum Wrightii (Crassulaceae), Lila D. Kerr, Dennis A. Gravatt, Robert J. Wiggers

Faculty Publications

Several studies have supported the idea that anthocyanin accumulation may be a possible protection mechanism in plants against DNA damage caused by ultraviolet radiation (UV). This study explored the accumulation of anthocyanins in the adventitious root tips of Sedum wrightii using the following treatments: UVA, UVA+low UVB, and UVA+high UVB. Following exposure to UV radiation, samples were analyzed for anthocyanin accumulation using an ethanol extraction procedure. Using ELISA, additional root samples were analyzed for indicators of DNA damage: cyclobutane pyrimidine dimers (CPDs) and pyrimidine (6-4) pyrimidone dimers (6-4 PPs). The anthocyanin concentrations were significantly higher in the UVA + high …

Integrating Drone Technology With Gps Data Collection To Enhance Forestry Students Interactive Hands-On Field Experiences, Daniel Unger, I-Kuai Hung, Yanli Zhang, David Kulhavy

Faculty Publications

Undergraduate students pursuing a Bachelor of Science in Forestry (BSF) at Stephen F. Austin State University (SFA) within the Arthur Temple College of Forestry and Agriculture (ATCOFA) attend an intensive 6-week hands-on instruction in applied field methods. The second week of field station is focused on land measurement activities to introduce students to practical, hands-on, and technology based ways to survey forest boundaries. On Monday of the second week students are introduced to the concepts of how to use a handheld compass to navigate from point to point, use a consumer-grade handheld Global Positioning System (GPS) unit for collecting the …

Site Factors Influence On Herbaceous Understory Diversity In East Texas Pinus Palustris Savannas, Brooke Mccalip, Brian Oswald, Kathryn R. Kidd, Yuhi Weng, Kenneth Farrish Phd

Faculty Publications

Longleaf pine (Pinus palustris) savannas were once dominant across the southeastern U.S., including East Texas and parts of western and central Louisiana. The diverse understory associated with these historical savannas may occasionally be seen today, but not often in longleaf pine ecosystems. This project aimed to define east Texas site characteristics that are necessary to support these ecosystems with a dense and diverse herbaceous understory with little to no midstory cover. Fifty-nine plots across three study sites were established to evaluate the influence of overstory cover, basal area, aspect, elevation, and slope on the number of plant genera present. Forest …

Accuracy Of Unmanned Aerial System (Drone) Height Measurements, Daniel Unger, I-Kuai Hung, David Kulhavy, Yanli Zhang, Kai Busch-Peterson

Faculty Publications

Vertical height estimates of earth surface features using an Unmanned Aerial System (UAS) are important in natural resource management quantitative assessments. An important research question concerns both the accuracy and precision of vertical height estimates acquired with a UAS and to determine if it is necessary to land a UAS between individual height measurements or if GPS derived height versus barometric pressure derived height while using a DJI Phantom 3 would affect height accuracy and precision. To examine this question, height along a telescopic height pole on the campus of Stephen F. Austin State University (SFASU) were estimated at 2, …

Integrating Unmanned Aircraft Systems To Measure Linear And Areal Features Into Undergraduate Forestry Education, Reid Viegut, David Kulhavy, Daniel Unger, I-Kuai Hung, Brian Humphreys

Faculty Publications

The use of Unmanned Aircraft Systems (UAS) in undergraduate forestry education continues to expand and develop. Accuracy of data collection is an important aspect of preparation for "society-ready" foresters to meet the complex sustainable environment managing for ecological, social and economic interests. Hands-on use of a DJI Phantom 4 Pro UAS by undergraduates to measure the length and area of 30 linear features and areal features on Earth's surface were estimated. These measurements were compared (measured within the ArcMap 10.5.2 interface) to hyperspectral Pictometry imagery measured on the web-based interface and the Google Earth Pro interface. Each remotely estimated measurement …

Surface And Subsurface Tillage Effects On Mine Soil Properties And Vegetative Response, H. Z. Angel, Jeremy Stovall, Hans Michael Williams, Kenneth W. Farrish, Brian P. Oswald, J. L. Young

Faculty Publications

Soil compaction is an important concern for surface mine operations that require heavy equipment for land reclamation. Excessive use of rubber-tired equipment, such as scraper pans, may cause mine soil compaction and hinder the success of revegetation efforts. However, information is limited on management strategies for ameliorating the potential compacting effects of scraper pans, particularly during site preparation for loblolly pine (Pinus taeda L.) plantations. Three forms of tillage and one control were replicated five times on surface mined land in the west Gulf Coastal Plain: no tillage (NT), disking (D), single-ripping + disking (R+D), and cross-ripping + disking …

Food Habits Of Sympatric Pitvipers From The West Gulf Coastal Plain, Usa, Christopher M. Schalk, Toni Trees, Joshua B. Pierce, D. Craig Rudolph

Faculty Publications

Widespread species that occupy multiple communities exhibit geographic variation in their natural history due to the ecological context of the local community. An animal’s food habitats are a central component to understanding its natural history and ecological role within its community—information that is critical to understanding resource needs of a species, mechanisms of species coexistence, and energy flow in food webs (Litvaitis 2000; Schalk et al. 2014). This information is also crucial for predicting the response of populations to changes in resource availability and, if necessary, inform mitigation strategies (Holycross and Mackessy 2002)

De-Extinction And The Conception Of Species, Leonard Finkelman

Faculty Publications

Developments in genetic engineering may soon allow biologists to clone organisms from extinct species. The process, dubbed “de-extinction,” has been publicized as a means to bring extinct species back to life. For theorists and philosophers of biology, the process also suggests a thought experiment for the ongoing “species problem”: given a species concept, would a clone be classified in the extinct species? Previous analyses have answered this question in the context of specific de-extinction technologies or particular species concepts. The thought experiment is given more comprehensive treatment here. Given the products of three de-extinction technologies, twenty-two species concepts are “tested” …

Modeling The Disappearance Of The Neanderthals Using Concepts Of Population Dynamics And Ecology, Michael F. Roberts, Stephen E. Bricher

Faculty Publications

Current hypotheses regarding the disappearance of Neanderthals (NEA) in Europe fall into two main categories: climate change, and competition. Here we review current research and existing mathematical models that deal with this question, and we propose an approach that incorporates and permits the investigation of the current hypotheses. We have developed a set of differential equations that model population dynamics of anatomically modern humans (AMH) and NEA, their ecological relations to prey species, and their mutual interactions. The model allows investigators to explore each of the two main categories or combinations of both, as well as various forms of competition …

Pkc-Dependent Phosphorylation Of Munc18a At Ser313 In Activated Rbl-2h3 Cells, Pratikshya Adhikari, Hao Xu

Faculty Publications

Protein Kinase C (PKC) regulates the release of pro-inflammatory compounds from IgE/antigen-activated mast cells by unknown mechanisms. In this study, we show for the first time that PKC inhibitor Ro-03-0432, which inhibits RBL-2H3 exocytosis/degranulation in a concentration-dependent fashion, prevents the phosphorylation of membrane fusion factor Munc18a at Ser 313. Our study provides fresh evidence that PKC-dependent protein phosphorylation may contribute to the intricate regulation of mast cell degranulation by directly targeting the fusion factors.

Walking In Minimalist Shoes Is Effective For Strengthening Foot Muscles, Sarah T. Ridge, Mark T. Olsen, Dustin A. Bruening, Kevin Jurgensmeier, David Griffin, Irene S. Davis, A. Wayne Johnson

Faculty Publications

Introduction: Weakness of foot muscles may contribute to a variety of loading-related injuries. Supportive footwear may contribute to intrinsic foot muscle weakness by reducing the muscles’ role in locomotion (e.g., absorbing forces and controlling motion). Increased stimulus to the foot muscles can be provided through a variety of mechanisms, including minimalist footwear and directed exercise.

Purpose: To determine the effect of walking in minimalist footwear or performing foot strengthening exercises on foot muscle size and strength.

Methods: Fifty-seven runners were randomly assigned to one of three groups—minimalist shoe walking (MSW), foot strengthening (FS) exercise, or control (C). All groups maintained …

From Big Ag To Campus Cafeterias: Intersections Of Food-Supply Networks As Technical Communication Pedagogy, Jessie Lynn Richards, Joshua Lenart, David Sumner, Douglas Christensen

Faculty Publications

This article presents a pedagogical approach to teaching technical and professional writing with an eye toward cultivating awareness and generating informed research among undergraduate students about food production and its various, intricate networks between Big Ag and campus cafeterias. Our pedagogy, influenced by interdisciplinary content, is designed to teach students to differentiate between food processes—such as production versus distribution and consumption—by viewing these networks as communicative practices rather than as inevitable chains or simple functions of one another. Our approach encourages students to locate and analyze differences between interdependent, but seemingly disparate pathways and to make visible communicative intersections that …

Amphibians And Reptiles Of The State Of San Luis Potosi, Mexico, With Comparisons With Adjoining States, J. A. Lemos-Espinal, Geoffrey R. Smith, G. A. Woolrich-Pina

Faculty Publications

No abstract provided.

Amphibians And Reptiles Of The State Of Durango, Mexico, With Comparisons With Adjoining States, J. A. Lemos-Espinal, Geoffrey R. Smith, H. Gadsden-Esparza, R. Valdez-Lares, G. A. Woolrich-Pina

Faculty Publications

No abstract provided.

Effects Of Nonnative Rainbow Trout On Two Species Of Endemic Mexican Amphibians, A. B. Estrella Zamora, Geoffrey R. Smith, J. A. Lemos-Espinal, G. A. Woolrich-Pina, R. Montoya Ayala

Faculty Publications

No abstract provided.

Comparison Of Varying Heel To Toe Differences And Cushion To Barefoot Running In Novice Minimalist Runners, Danny Moody, Iain Hunter, Sarah T. Ridge, J. William Myrer

Faculty Publications

There are many different types of footwear available for runners in today’s market. Many of these shoes claim to help runners run more efficiently by altering an individual’s stride mechanics. Minimalist footwear claims to aid runners run more on their forefeet whereas more traditional footwear provides more cushioning specifically for a heel first landing. The purpose of this paper was to determine if runners, who were accustomed to running in traditional footwear would alter their running mechanics while running acutely in various types of minimalist footwear. Twelve subjects, accustomed to running in traditional 12 mm heel/toe differential footwear, ran in …

Deep Learning Architectures For Multi-Label Classification Of Intelligent Health Risk Prediction, Andrew Maxwell, Runzhi Li, Bei Yang, Heng Weng, Aihua Ou, Huixiao Hong, Zhaoxian Zhou, Ping Gong, Chaoyang Zhang

Faculty Publications

No abstract provided.

Classifying Cancer Genome Aberrations By Their Mutually Exclusive Effects On Transcription, Jonathan B. Dayton, Stephen R. Piccolo

Faculty Publications

Background

Malignant tumors are typically caused by a conglomeration of genomic aberrations—including point mutations, small insertions, small deletions, and large copy-number variations. In some cases, specific chemotherapies and targeted drug treatments are effective against tumors that harbor certain genomic aberrations. However, predictive aberrations (biomarkers) have not been identified for many tumor types and treatments. One way to address this problem is to examine the downstream, transcriptional effects of genomic aberrations and to identify characteristic patterns. Even though two tumors harbor different genomic aberrations, the transcriptional effects of those aberrations may be similar. These patterns could be used to inform treatment …

Deep Experimental Profiling Of Microrna Diversity, Deployment, And Evolution Across The Drosophila Genus, Jaaven Mohammed, Alex S. Flynt, Alexandra M. Panzarino, Md Mosharrof Hossain Mondal, Matthew Decruz, Adam Siepel, Eric C. Lai

Faculty Publications

To assess miRNA evolution across the Drosophila genus, we analyzed several billion small RNA reads across 12 fruit fly species. These data permit comprehensive curation of species- and clade-specific variation in miRNA identity, abundance, and processing. Among well-conserved miRNAs, we observed unexpected cases of clade-specific variation in 5′ end precision, occasional antisense loci, and putatively noncanonical loci. We also used strict criteria to identify a large set (649) of novel, evolutionarily restricted miRNAs. Within the bulk collection of species-restricted miRNAs, two notable subpopulations are splicing-derived mirtrons and testes-restricted, recently evolved, clustered (TRC) canonical miRNAs. We quantified miRNA birth and death …

Homogenization Techniques For Population Dynamics In Strongly Heterogeneous Landscapes, Brian P. Yurk, Christina A. Cobbold

Faculty Publications

An important problem in spatial ecology is to understand how population-scale patterns emerge from individual-level birth, death, and movement processes. These processes, which depend on local landscape characteristics, vary spatially and may exhibit sharp transitions through behavioural responses to habitat edges, leading to discontinuous population densities. Such systems can be modelled using reaction–diffusion equations with interface conditions that capture local behaviour at patch boundaries. In this work we develop a novel homogenization technique to approximate the large-scale dynamics of the system. We illustrate our approach, which also generalizes to multiple species, with an example of logistic growth within a periodic …

Genome Sequences Of Mycobacteriophages Amgine, Amohnition, Bella96, Cain, Darthp, Hammy, Krueger, Lasthope, Peanam, Phelpsodu, Phrank, Sirphilip, Slimphazie, And Unicorn, Kirk R. Anders, Nazir Barekzi, Aaron A. Best, Gregory D. Frederick, Dmitri V. Mavrodi, Edwin Vasquez, Sea-Phages, Nana Yaa A. Amoh, Frederick N. Baliraine, William J. Buchser, Thomas P. Cast, Carmen E. Chamberlain, Hui-Min Chung, William A. D'Angelo, Christian T. Farris, Mariceli Fernandez-Martinez, Haley D. Fischman, Mark H. Forsyth, Anna G. Fortier, Kara F. Gallo, Greta J. Held, Miguel A. Lomas, Natalia Y. Maldonado-Vasquez, Claudia H. Moonsammy, Peace Namboote, Sudip Paudel, Sarah-Elizabeth Polley, Gabriella Reyes, Michael R. Rubin, Margaret S. Saha, Joseph Stukey, Tristan D. Tobias, Rebecca A. Garlena, Ty H. Stoner, Steven G. Cresawn, Deborah Jacobs-Sera, Welkin H. Pope, Daniel A. Russell, Graham F. Hatfull

Faculty Publications

We report the genome sequences of 14 cluster K mycobacteriophages isolated using Mycobacterium smegmatis mc²155 as host. Four are closely related to subcluster K1 phages, and 10 are members of subcluster K6. The phage genomes span considerable sequence diversity, including multiple types of integrases and integration sites.