Life Sciences Commons^™

Facially Amphiphilic Polyionene Biocidal Polymers Derived From Lithocholic Acid, Mitra S. Ganewatta, Md Anisur Rahman, Louis Mercado, Tinom Shokfai, Alan W. Decho, Theresa M. Reineke, Chuanbing Tang

Faculty Publications

Bacterial infections have become a global issue that requires urgent attention, particularly regarding to emergence of multidrug resistant bacteria. We developed quaternary amine-containing antimicrobial poly(bile acid)s that contain a hydrophobic core of lithocholic acid in the main-chain. Interestingly, by choosing appropriate monomers, these cationic polymers can form core-shell micelles. These polymers exhibited biocidal activity against both Gram-positive and Gram-negative bacterial species. It is demonstrated that the micelles can deliver hydrophobic antibiotics that functionally have dual antimicrobial activities. Cytotoxicity assays against HeLa cells showed dosage-dependent toxicity for polymers with longer linkers.

Assessing Natural Infection With Zika Virus In The Southern House Mosquito, Culex Quinquefasciatus, During 2016 In Puerto Rico, Donald A. Yee, Rebeca De Jesus Crespo, Fiona F. Hunter, Fengwei Bai

Faculty Publications

The epidemic of Zika in the Western hemisphere has led to intense investigations of all species important in the transmission of Zika virus (ZikV), including putative mosquito vectors. Although evidence points to Stegomyia (= Aedes) (Diptera: Culicidae) mosquitoes as the primary vectors in nature among humans, there remains the possibility that other common mosquito species may be implicated in the rapid spread of the virus. Herein, field-caught Culex quinquefasciatus (Diptera: Culicidae) collected during June 2016 in different neighbourhoods in San Juan, Puerto Rico were examined for the presence of natural infection with ZikV. Stegomyia aegypti (= Aedes aegypti) …

The Effect Of Light Conditions On Interpreting Oil Composition Engineering In Arabidopsis Seeds, Nischal Karki, Philip D. Bates

Faculty Publications

Arabidopsis thaliana is the most developed and utilized model plant. In particular, it is an excellent model for proof‐of‐concept seed oil engineering studies because it accumulates approximately 37% seed oil by weight, and it is closely related to important Brassicaceae oilseed crops. Arabidopsis can be grown under a wide variety of conditions including continuous light; however, the amount of light is strongly correlated with total seed oil accumulation. In addition, many attempts to engineer novel seed oil fatty acid compositions in Arabidopsis have reported significant reductions in oil accumulation; however, the relative reduction from the nontransgenic controls varies greatly within …

Feeding Patterns Of Two Commercially Important Fish Species Scomberoides Commersonnianus And S. Tol In The Northern Arabian Sea Coast Of Pakistan, Nazia Qamar, Sher Khan Panhwar, Ralf Riedel

Faculty Publications

Port landing of Scomberoides commersonnianus and S. tol were obtained between July 2013 and June 2015 for stomach content analysis. Analysis of prey composition was done using permutational analysis of variance (permanova), with species, life stage (juvenile and adults), gender, and weather (rainy and dry season) as factors. Patterns of empty stomachs were investigated to estimate feeding intensity. Feeding intensity was estimated with logistic regression, using the same independent variables as above. Prey importance was also investigated. Prey importance was assessed using a Wilcox Rank Correlation analysis on the Index of Relative Importance (IRI) by species and life-stage. Permanova analysis …

Toxicity Of Selected Acaricides To Honey Bees (Apis Mellifera) And Varroa (Varroa Destructor Anderson And Trueman) And Their Use In Controlling Varroa Within Honey Bee Colonies, Aleš Gregorc, Mohamed Alburaki, Blair Sampson, John Adamczyk

Faculty Publications

The efficacies of various acaricides in order to control a parasitic mite, the Varroa mite, Varroa destructor, of honey bees, were measured in two different settings, namely, in laboratory caged honey bees and in queen-right honey bee colonies. The Varroa infestation levels before, during, and after the acaricide treatments were determined in two ways, namely: (1) using the sugar shake protocol to count mites on bees and (2) directly counting the dead mites on the hive bottom inserts. The acaricides that were evaluated were coumaphos, tau-fluvalinate, amitraz, thymol, and natural plant compounds (hop acids), which were the active ingredients. The …

Metastatic Colon Adenocarcinoma Has A Significantly Elevated Expression Of Il-10 Compared With Primary Colon Adenocarcinoma Tumors, Michelle H. Townsend, Abigail M. Felsted, Stephen R. Piccolo, Richard A. Robison, Kim Oneill

Faculty Publications

Classical anti-inflammatory cytokines are known to play a role in both cancer progression as well as cancer elimination. We evaluated the anti-inflammatory cytokines IL-10 and TGF-β in patients with colon adenocarcinoma and metastatic colon adenocarcinoma using immunohistochemical assays to determine the expression of the cytokines between various malignant tissues. We found tissues stained with TGF-β showed no significant upregulation within malignant tumors when compared with normal tissue controls. We observed high levels of TGF-β presence in most tissues similar to GAPDH expression. Within both colon adenocarcinoma and metastatic carcinomas there was a significant variability among patients in the expression of …

Physical Processes Dictate Early Biogeochemical Dynamics Of Soil Pyrogenic Organic Matter In A Subtropical Forest Ecosystem, Jason Stuart, Russell Anderson, Patrick Lazzarino, Kevin A. Kuehn, Omar R. Harvey

Faculty Publications

Quantifying links between pyOM dynamics, environmental factors and processes is central to predicting ecosystem function and response to future perturbations. In this study, changes in carbon (TC), nitrogen (TN), pH, and relative recalcitrance (R50) for pineand cordgrass-derived pyOM were measured at 3–6 weeks intervals throughout the first year of burial in the soil. Objectives were to (1) identify key environmental factors and processes driving early-stage pyOM dynamics, and (2) develop quantitative relationships between environmental factors and observed changes in pyOM properties. The study was conducted in sandy soils of a forested ecosystem within the Longleaf pine range of the United …

A Time Course Study Of Rotavirus-Infected Intestinal Cells Treated With Stilbenoids And The Regulation Of Apoptosis, Rebecca D. Parr, Caleb M. Witcher, Rebekah Napier-Jameson, Hannah L. Wilson, Stormey Wisdom, Macie N. Mattila, Essence B. Strange, Josephine Taylor, Beatrice Clack, Fabricio Medina-Bolivar, Judith M. Ball

Faculty Publications

This is a time course study of virus –host interactions that are modified with the addition of two small natural products. They appear to effect virus replication and the host response to the infection.

Spatial Dynamics And Mixing Of Bluefin Tuna In The Atlantic Ocean And Mediterranean Sea Revealed Using Next-Generation Sequencing, Gregory N. Puncher, Alessia Cariani, Gregory E. Maes, Jeroen Van Houdt, Koen Herten, Rita Cannas, Naiara Rodriguez-Ezpeleta, Aitor Albania, Andone Estonba, Molly Lutcavage, Alex Hanke, Jay Rooker, James S. Franks, Joseph M. Quattro, Gualtiero Basilone, Igaratza Fraile, Urtzi Laconcha, Nicolas Goñi, Ai Kimoto, David Macías, Francisco Alemany, Simeon Deguara, Salem W. Zgozi, Fulvio Garibaldi, Isik K. Oray, Firdes Saadet Karakulak, Noureddine Abid, Miguel N. Santos, Piero Addis, Haritz Arrizabalaga, Fausto Tinti

Faculty Publications

The Atlantic bluefin tuna is a highly migratory species emblematic of the challenges associated with shared fisheries management. In an effort to resolve the species’ stock dynamics, a genomewide search for spatially informative single nucleotide polymorphisms (SNPs) was undertaken, by way of sequencing reduced representation libraries. An allele frequency approach to SNP discovery was used, combining the data of 555 larvae and young‐of‐the‐year (LYOY) into pools representing major geographical areas and mapping against a newly assembled genomic reference. From a set of 184,895 candidate loci, 384 were selected for validation using 167 LYOY. A highly discriminatory genotyping panel of 95 …

Evaluating The Role Of Large Jellyfish And Forage Fishes As Energy Pathways, And Their Interplay With Fisheries, In The Northern Humboldt Current Systems, Luciano M. Chiaverino, Kelly L. Robinson, Jorge Tam, James J. Ruzicka, Javier Quiñones, Katrina Aleksa, Frank J. Hernandez Jr., Richard D. Brodeur, Robert T. Leaf, Shin-Ichi Uye, Mary Beth Decker, Marcelo Acha, Hermes Mianzan, William M. Graham

Faculty Publications

Large jellyfish are important consumers of plankton, fish eggs and fish larvae in heavily fished ecosystems worldwide; yet they are seldom included in fisheries production models. Here we developed a trophic network model with 41 functional groups using ECOPATH re-expressed in a donor-driven, end-to-end format to directly evaluate the efficiency of large jellyfish and forage fish at transferring energy to higher trophic levels, as well as the ecosystem-wide effects of varying jellyfish and forage fish consumption rates and fishing rates, in the Northern Humboldt Current system (NHCS) off of Peru. Large jellyfish were an energy-loss pathway for high …

Threshold-Dependent Repression Of Spl Gene Expression By Mir156/Mir157 Controls Vegetative Phase Change In Arabidopsis Thaliana, Jia He, Mingli Xu, Matthew R. Willmann, Kevin Mccormick, Tieqiang Hu, Li Yang, Colby G. Starker, Daniel F. Voytas, Blake C. Meyers, R. Scott Poethig

Faculty Publications

Vegetative phase change is regulated by a decrease in the abundance of the miRNAs, miR156 and miR157, and the resulting increase in the expression of their targets, SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) transcription factors. To determine how miR156/miR157 specify the quantitative and qualitative changes in leaf morphology that occur during vegetative phase change, we measured their abundance in successive leaves and characterized the phenotype of mutations in different MIR156 and MIR157 genes. miR156/miR157 decline rapidly between leaf 1&2 and leaf 3 and decrease more slowly after this point. The amount of miR156/miR157 in leaves 1&2 greatly exceeds the …

High Male Sexual Investment As A Driver Of Extinction In Fossil Ostracods, Maria João Fernandes Martins, T. Markham Puckett, Rowan Lockwood, John P. Swaddle, Gene Hunt

Faculty Publications

Sexual selection favours traits that confer advantages in the competition for mates. In many cases, such traits are costly to produce and maintain, because the costs help to enforce the honesty of these signals and cues. Some evolutionary models predict that sexual selection also produces costs at the population level, which could limit the ability of populations to adapt to changing conditions and thus increase the risk of extinction. Other models, however, suggest that sexual selection should increase rates of adaptation and enhance the removal of deleterious mutations, thus protecting populations against extinction. Resolving the conflict between these models is …

Editing Life: Modern Biology And Biblical Principles, Peter J. Lyons

Faculty Publications

No abstract provided.

A Plant-Produced Vaccine Protects Mice Against Lethal West Nile Virus Infection Without Enhancing Zika Or Dengue Virus Infectivity, Huafang Lai, Amber M. Paul, Haiyan Sun, Junyun He, Ming Yang, Fengwei Bai

Faculty Publications

West Nile virus (WNV) has caused multiple global outbreaks with increased frequency of neuroinvasive disease in recent years. Despite many years of research, there are no licensed therapeutics or vaccines available for human use. One of the major impediments of vaccine development against WNV is the potential enhancement of infection by related flaviviruses in vaccinated subjects through the mechanism of antibody-dependent enhancement of infection (ADE). For instance, the recent finding of enhancement of Zika virus (ZIKV) infection by pre-exposure to WNV further complicates the development of WNV vaccines. Epidemics of WNV and the potential risk of ADE by current vaccine …

Long-Term Irrigation Affects The Dynamics And Activity Of The Wheat Rhizosphere Microbiome, Dmitri V. Mavrodi, Olga Mavrodi, Liam D.H. Elbourne, Sasha Tetu, Robert F. Bonsall, James Parejko, Mingming Yang, Ian T. Paulsen, David M. Weller, Linda S. Thomashow

Faculty Publications

The Inland Pacific Northwest (IPNW) encompasses 1. 6 million cropland hectares and is a major wheat-producing area in the western United States. The climate throughout the region is semi-arid, making the availability of water a significant challenge for IPNW agriculture. Much attention has been given to uncovering the effects of water stress on the physiology of wheat and the dynamics of its soilborne diseases. In contrast, the impact of soil moisture on the establishment and activity of microbial communities in the rhizosphere of dryland wheat remains poorly understood. We addressed this gap by conducting a three-year field study involving wheat …

Does Body Size Affect Fitness The Same Way In Males And Females? A Test Of Multiple Fitness Components, Ashlee N. Smith, Mark C. Belk

Faculty Publications

Body size generally has an important relationship with fitness, whereby larger body size leads to an increase in fitness through competition, reproductive output and survivorship. However, the traits through which body size increases fitness often differ between the sexes. We tested for the effects of body size on fitness in both sexes using three separate experiments on competitive ability, reproductive output and starvation resistance in the burying beetle Nicrophorus marginatus. Results varied between sexes as follows: (1) larger body size increased competitive ability differentially between sexes; (2) female body size, but not male body size, significantly affected reproductive output …

Descriptive Density Models Of Scyphozoan Jellyfish In The Northern Gulf Of Mexico, Katrina T. Aleksa, Redwood W. Nero, Jerry D. Wiggert, William M. Graham

Faculty Publications

Jellyfish play an important role in the food web of many coastal environments but are generally considered a nuisance to scientific fieldwork and industrial and economic trades. A better understanding of how jellyfish densities and distributions are affected by environmental parameters could elucidate population trends and provide assistance in ecological research and undesirable human interactions. In this study, abundance data for scyphozoan medusae, Chrysaora sp. and Aurelia spp., were extracted from Southeast Area Monitoring and Assessment Program trawling surveys for the northern Gulf of Mexico (nGoM) during the summer and fall months. Both in situ and satellite oceanographic measurements were …

Andale Pittsburgh: Results Of A Promotoraled, Home-Based Intervention To Promote A Healthy Weight In Latino Preschool Children, Sharon E. Taverno Ross, Bethany Barone Gibbs, Patricia I. Documet, Russell R. Pate

Faculty Publications

Background: Latino preschool children have higher rates of obesity than preschool children from other racial/ ethnic groups; however, few effective, culturally appropriate interventions exist targeting this group. The purpose of this study was to test the feasibility of a 10-week, promotora-mediated, home-based intervention to promote a healthy weight in Latino preschool children.

Methods: Trained promotoras (community health workers) delivered 10, 90-min weekly interactive and tailored sessions to Latino families living in Allegheny County. Participants were recruited through promotoras’ own social networks and community gatherings, flyers, and word of mouth. Primary outcome measures included child body mass index (BMI) z-score and …

Spatial Extinction Date Estimation: A Novel Method For Reconstructing Spatiotemporal Patterns Of Extinction And Identifying Potential Zones Of Rediscovery, Colin J. Carlson, Kevin R. Burgio, Tad Dallas, Alexander L. Bond

Faculty Publications

1. The estimation of extinction dates from limited and incomplete sighting records

is a key challenge in conservation (when experts are uncertain whether a species has

gone extinct) and historical ecology (when the date and mechanism of extinction is

controversial).

2. We introduce a spatially-explicit method of interpolating extinction date estima8 tors, allowing users to estimate spatiotemporal surfaces of population persistence 9 from georeferenced sighting data of variable quality.

3. We present the R package spatExtinct, which produces spatially-explicit extinction date surfaces from geolocated sightings, including options for custom randomization schemes to improve accuracy with limited datasets. We use simulations …

Physico-Chemical Characteristics Of Evaporating Respiratory Fluid Droplets, Eric P. Vejerano, Linsey C. Marr

Faculty Publications

The detailed physico-chemical characteristics of respiratory droplets in ambient air, where they are subject to evaporation, are poorly understood. Changes in the concentration and phase of major components in a droplet—salt (NaCl), protein (mucin) and surfactant (dipalmitoylphosphatidylcholine)—may affect the viability of any pathogens contained within it and thus may affect the efficiency of transmission of infectious disease by droplets and aerosols. The objective of this study is to investigate the effect of relative humidity (RH) on the physico-chemical characteristics of evaporating droplets of model respiratory fluids. We labelled these components in model respiratory fluids and observed evaporating droplets suspended on …

Diverse Exocytic Pathways For Mast Cell Mediators, Hao Xu, Na-Ryum Bin, Shuzo Sugita

Faculty Publications

Mast cells play pivotal roles in innate and adaptive immunities but are also culprits in allergy, autoimmunity and cardiovascular diseases. Mast cells respond environmental changes by initiating regulated exocytosis/secretion of various biologically active compounds called mediators (e.g., proteases, amines and cytokines). Many of these mediators are stored in granules/lysosomes and rely on an intricate degranulation process for release. Mast cell stabilizers (such as sodium cromoglicate) which prevent such degranulation process have therefore been clinically approved to treat asthma and allergic rhinitis. However, it has become increasingly clear that different mast cell diseases often involve multiple mediators, which seem to rely …

Mixing It Up In The Ocean Carbon Cycle And The Removal Of Refractory Dissolved Organic Carbon, Yuan Shen, Ronald Benner

Faculty Publications

A large quantity of reduced carbon is sequestered in the ocean as refractory dissolved molecules that persist through several circuits of global overturning circulation. Key aspects of the cycling of refractory dissolved organic carbon (DOC) remain unknown, making it challenging to predict how this large carbon reservoir will respond to climate change. Herein we investigate mechanisms that remove refractory DOC using bioassay experiments with DOC isolated from surface, mesopelagic and deep waters of the Atlantic Ocean. The isolated DOC was refractory to degradation by native microbial communities, even at elevated concentrations. However, when the refractory DOC was introduced to a …

Expansion Of The (Bb)>Ru Metallacycle With Coinage Metal Cations: Formation Of B-M-Ru-B (M= Cu, Ag, Au) Dimetalacyclodiboryls†, Bennett J. Eleazer, Mark D. Smith, Alexey A. Popov, Dmitry V. Peryshkov

Faculty Publications

In this work, we introduce a novel approach for the selective assembly of heterometallic complexes by unprecedented coordination of coinage metal cations to strained single ruthenium–boron bonds on a surface of icosahedral boron clusters. M(I) cations (M ¼ Cu, Ag, and Au) insert into B–Ru bonds of the (BB)–carboryne complex of ruthenium with the formation of four-membered B–M–Ru–B metalacycles. Results of theoretical calculations suggest that bonding within these metalacycles can be best described as unusual three-center-two-electron B–M/Ru interactions that are isolobal to B–H/Ru borane coordination for M ¼ Cu and Ag, or the pairs of two-center-two electron B–Au and Au–Ru …

Stepping It Up: Walking Behaviors In Children Transitioning From 5th To 7th Grade, Sharon E. Taverno Ross, Morgan N. Clennin, Marsha Dowda, Natalie Colabianchi, Russell R. Pate

Faculty Publications

The purpose of this study was to (1) describe children’s walking behaviors in 5th to 7th grade and change over time and (2) examine associations between walking behaviors and Walk Score®. Participants consisted of n = 586 students from the Transitions and Activity Changes in Kids (TRACK) Study. Children reported any walking behavior (e.g., exercise and transportation) over the past five days. Walk Score was calculated based on children’s home address. Descriptive statistics summarized walking behaviors by gender and time, and repeated measure mixed models examined the relationship between walking behaviors and Walk Score. Approximately 46.8% and 19.2% of 5th …

A Bio-Based Pro-Antimicrobial Polymer Network Via Degradable Acetal Linkages, Douglas V. Amato, Dahlia N. Amato, Logan T. Blancett, Olga V. Mavrodi, William B. Martin, Sarah N. Swilley, Michael J. Sandoz, Glenmore Shearer, Dmitri V. Mavrodi, Derek Patton

Faculty Publications

The synthesis of a fully degradable, bio-based, sustained release, pro-antimicrobial polymer network comprised of degradable acetals (PANDA) is reported. The active antimicrobial agent – p-anisaldehyde (pA) (an extract from star anise) – was converted into a UV curable acetal containing pro-antimicrobial monomer and subsequently photopolymerized into a homogenous thiol-ene network. Under neutral to acidic conditions (pH < 8), the PANDAs undergo surface erosion and exhibit sustained release of pA over 38 days. The release of pA from PANDAs was shown to be effective against both bacterial and fungal pathogens. From a combination of confocal microscopy and transmission electron microscopy, we observed that the released pA disrupts the cell membrane. Additionally, we demonstrated that PANDAs have minimal cytotoxicity towards both epithelial cells and macrophages. Although a model platform, these results point to promising pathways for the design of fully degradable sustained-release antimicrobial systems with potential applications in agriculture, pharmaceuticals, cosmetics, household/personal care, and food industries.

Rewired Rnai-Mediated Genome Surveillance In House Dust Mites, Mosharrof H. Mondal, Pavel Klimov, Alex S. Flynt

Faculty Publications

House dust mites are common pests with an unusual evolutionary history, being descendants of a parasitic ancestor. Transition to parasitism is frequently accompanied by genome rearrangements, possibly to accommodate the genetic change needed to access new ecology. Transposable element (TE) activity is a source of genomic instability that can trigger large-scale genomic alterations. Eukaryotes have multiple transposon control mechanisms, one of which is RNA interference (RNAi). Investigation of the dust mite genome failed to identify a major RNAi pathway: the Piwi-associated RNA (piRNA) pathway, which has been replaced by a novel small-interfering RNA (siRNA)-like pathway. Co-opting of piRNA function by …

Dietary Overlap Between Jellyfish And Forage Fish In The Northern Gulf Of Mexico, Isabella D'Ambra, William M. Graham, Ruth H. Carmichael, Frank J. Hernandez Jr.

Faculty Publications

Despite the speculations that jellyfish (hydromedusae, siphonophores, scyphomedusae and ctenophores) may compete with forage fish for prey, there are few direct comparisons of their diets. To determine the dietary overlap between Aurelia sp. (Cnidaria, Scyphozoa) and Brevoortia patronus (Goode, 1878) (Pisces, Clupeidae) in the northern Gulf of Mexico, we collected monthly samples in Louisiana, Mississippi and Alabama coastal waters (USA) during summer and early fall 2009−2010. We determined carbon and nitrogen stable isotope ratios in predators and their potential prey, including small plankton (<200 µm) and mesozooplankton (200− 2000 µm), and identified prey in the stomachs of adult Aurelia sp. and B. patronus. Trophic niche overlap was defined using the stable isotope Bayesian …

Experimental Evidence Of A Pathogen Invasion Threshold, Tad Dallas, Martin Krkošek, John M. Drake

Faculty Publications

Host density thresholds to pathogen invasion separate regions of parameter space corresponding to endemic and disease-free states. The host density threshold is a central concept in theoretical epidemiology and a common target of human and wildlife disease control programmes, but there is mixed evidence supporting the existence of thresholds, especially in wildlife populations or for pathogens with complex transmission modes (e.g. environmental transmission). Here, we demonstrate the existence of a host density threshold for an environmentally transmitted pathogen by combining an epidemiological model with a microcosm experiment. Experimental epidemics consisted of replicate populations of naive crustacean zooplankton (Daphnia dentifera …

Reliability Of Pulse Waveform Separation Analysis Responses To An Orthostatic Challenge, Lee Stoner, Keeron Stone, Erik D. Hanson, James Faulkner, Simon Fryer, Daniel Credeur

Faculty Publications

Cardiovascular autonomic nervous system function can be assessed using an orthostatic challenge to induce arterial wave reflection. While arterial reflection is typically estimated using a central augmentation index, a superior estimation can be obtained using pulse wave separation analysis to estimate the aortic backward pressure wave (Pb). However, to be of value in a clinical or research setting, an assessment tool must be precise (reliable). Therefore, this study sought to determine the measurement precision of Pb responses to a modified tilt-table test. Twenty healthy adults (26.4 year (SD 5.2), 24.7kg/m² (SD 3.8), 55% female) were tested in a fasted …

Temporal Migration Patterns Between Natal Locations Of Ruby-Throated Hummingbirds (Archilochus Colubris) And Their Gulf Coast Stopover Site, Theodore J. Zenzal Jr., Andrea J. Contina, Jeffrey F. Kelly, Frank R. Moore

Faculty Publications

Background

Autumn latitudinal migrations generally exhibit one of two different temporal migration patterns: type 1 where southern populations migrate south before northern populations, or type 2 where northern populations overtake southern populations en route. The ruby-throated hummingbird (Archilochus colubris) is a species with an expansive breeding range, which allows opportunities to examine variation in the timing of migration. Our objective was to determine a relationship between natal origin of ruby-throated hummingbirds and arrival at a Gulf coast stopover site; and if so, what factors, such as differences in body size across the range as well as the …