Life Sciences Commons^™

Acute Blood Flow Responses To Varying Blood Flow Restriction Pressures In The Lower Limbs, Bjoern Hornikel, Keith S. Saffold, Jacob A. Mota, Michael R. Esco, Michael V. Fedewa, Stefanie A. Wind, Lee J. Winchester

International Journal of Exercise Science

International Journal of Exercise Science 16(2): 118-128, 2023. The purpose of this study was to investigate lower limb blood flow responses under varying blood flow restriction (BFR) pressures based on individualized limb occlusion pressures (LOP) using a commonly used occlusion device. Twenty-nine participants (65.5% female, 23.8 ± 4.7 years) volunteered for this study. An 11.5cm tourniquet was placed around participants’ right proximal thigh, followed by an automated LOP measurement (207.1 ± 29.4mmHg). Doppler ultrasound was used to assess posterior tibial artery blood flow at rest, followed by 10% increments of LOP (10-90% LOP) in a randomized order. All data were …

A Pilot Study On Cannabidiol (Cbd) And Eccentric Exercise: Impact On Inflammation, Performance, And Pain, Whitley J. Stone, Danilo Tolusso, Guillermo Pacheco, Shea M. Brgoch, Van T. Nguyen

International Journal of Exercise Science

International Journal of Exercise Science 16(2): 109-117, 2023. Cannabidiol (CBD) is a non-psychoactive cannabinoid purported to reduce symptoms of discomfort. Individuals are now using CBD to treat symptoms of multiple sclerosis, seizures, and chronic pain. Animal models indicate that CBD may be effective at reducing inflammation post fatiguing exercise. However, little evidence is available to evaluate these findings in humans. Therefore, the purpose of this investigation was to evaluate the impact of two doses of CBD oil on inflammation (IL-6), performance, and pain after an eccentric loading protocol. Participants (n = 4) participated in three conditions (placebo, low dose, …

Effects Of Acute Exercise On Affect In Females With Substance Use Disorder, Victoria A. Torok, Christi B. Brewer, Hayley N. Lake

International Journal of Exercise Science

International Journal of Exercise Science 16(5): 95-108, 2023. Negative mood states experienced during the withdrawal stage of substance dependence have been associated with relapse in persons suffering from substance use disorder (SUD). Exercise is gaining attention as an adjunct therapy for SUD due to its ability to alleviate negative mood states experienced during withdrawal. The purpose of this study was to investigate the effects of acute, controlled bouts of aerobic and resistance exercise versus sedentary control (quiet reading) on positive affect (PA) and negative affect (NA) in females undergoing SUD treatment at inpatient facilities. Females (n=11; 34 + 8 yrs) …

Improvements In Flexibility Depending On Stretching Duration, Konstantin Warneke, Klaus Wirth, Michael Keiner, Stephan Schiemann

International Journal of Exercise Science

International Journal of Exercise Science 16(4): 83-94, 2023. To improve flexibility, stretching is most commonly used and in training interventions duration-dependent effects are hypothesized. However, there are strong limitations in used stretching protocols in most studies, particularly regarding documentation of intensity and performed procedure. Thus, aim of this study was to compare different stretching durations on flexibility in the plantar flexors and to exclude potential biases. Eighty subjects were divided into four groups performing daily stretching training of 10min (IG10), 30min (IG30) and 1h (IG60) and one control group (CG). Flexibility was measured in bended and extended knee joint. Stretching …

The Relationship Between Health And Movement Screens And Field-Based Physical Fitness Tests In Reserve Officer Training Corps Students, Lukas Krumpl, Nickolai J. P. Martonick, Youngmin Chun, Ann F. Brown, Joshua P. Bailey

International Journal of Exercise Science

International Journal of Exercise Science 16(4): 42-52, 2023. The purpose of the present study was to evaluate the relationship between scores achieved in physical fitness tests and outcomes of health and movement screens (HMS) in ROTC students. Twenty-eight students (20 males: 21.8 yrs [± 3.4] & 8 females: 20.7 yrs [± 1.8]) enrolled in an ROTC branch (Army, Air Force, Navy, or Marines) completed a series of screens, including body composition analysis via Dual-Energy X-Ray Absorptiometry (DXA), balance and functional movement tasks via a lower-quarter Y-Balance test, and knee and hip joint concentric strength testing on an isokinetic dynamometer. Official …

The Effects Of Anabolic-Androgenic Steroids On Gene Expression In Skeletal Muscle: A Systematic Review, Luke M. Pelton, Stephen A. Maris, Joshua Loseke

International Journal of Exercise Science

International Journal of Exercise Science 16(3): 53-82, 2023. Anabolic-androgenic steroids (AAS) act via androgen receptor (AR) interaction to induce muscle protein synthesis. This process is achieved via altered gene expression via the Notch, Wnt, and Numb pathways and their interactions at the AR, manifesting in key skeletal muscle (SM) phenotypes such as morphology, ion conductance, and functionality. This review aims to report on the effects of AAS administration on gene expression in SM. Peer-reviewed empirical studies evaluating AAS administration on SM phenotypes and gene expression were considered for inclusion. The following databases were searched using a data range of Jan …

The Relationship Between Cognition, Preseason Hitting Assessments, And In-Game Batting Performance In Collegiate Baseball And Softball Players, Amanda Carrol, Tonya Krupp, Kara Tucker, Nicholas J. Siekirk, Bradley J. Kendall

International Journal of Exercise Science

International Journal of Exercise Science 16(6): 23-30, 2023. Successful hitting performance may be related to perceptual processing of visual information. The purpose of this investigation was to examine the relationship between preseason cognitive assessments, off-field preseason hitting assessment, and in-game batting performing in collegiate baseball and softball athletes. Collegiate varsity baseball (n = 10, 20.5 ± 1.0 years) and softball (n = 16, 20.3 ± 1.3 years) underwent Flanker Task and Trail Maker Tests A (TMT-A) and B (TMT-B) 24 hours prior to a pre-seasoning indoor hitting assessment. During pre-season hitting assessment, athletes selected 10 underhand pitches and …

Prediction Of Rowing Functional Threshold Power Using Body Mass, Blood Lactate And Gxt Peak Power Data., Eanna Mc Grath, Bernard Donne, Nicholas Mahony, Neil Fleming

International Journal of Exercise Science

International Journal of Exercise Science 16(4): 31-41, 2023. Functional Threshold Power (FTP) is a validated index of a maximal quasi steady-state cycling intensity. The central component of the FTP test is a maximal 20-min time-trial effort. A model to predict FTP from a cycling graded exercise test (m-FTP) was published that estimated FTP without the requirement of the exhaustive 20-min time-trial. The predictive model (m-FTP) was trained (developed to find the best combination of weights and bias) on a homogenous group of highly-trained cyclists and triathletes. This investigation appraised the external validity of the m-FTP model vis-à-vis the alternate modality …

A Comparison Of Muscle Recruitment Across Three Straight-Legged, Hinge-Pattern Resistance Training Exercises, Jeremy R. Dicus, Samuel H. Ellestad, Jesse E. Sheaffer, Carol A. Weber, Noah C. Novak, Michael E. Holmstrup

International Journal of Exercise Science

International Journal of Exercise Science 16(4): 12-22, 2023. Hinge exercises are critical to building a balanced resistance training program in concert with ‘knee-dominant’ (e.g., squat, lunge) exercises. Biomechanical differences between various straight-legged hinge (SLH) exercises may alter muscle activation. For example, a Romanian deadlift (RDL) is a closed-chain SLH, while a reverse hyperextension (RH) is open-chain. Likewise, the RDL offers resistance via gravity while the cable pull-through (CP) offers redirected-resistance through a pulley. A deeper understanding of the potential impact of these biomechanical differences between these exercises may improve their application to specific goals. Participants completed repetition-maximum (RM) testing on …

How Did Participating In A Prenatal Nutrition And Exercise Program Influence Postpartum Experiences During Covid-19?, Rozhan Momen, Maya Weinreich, Taniya S. Nagpal, Michelle F. Mottola

International Journal of Exercise Science

International Journal of Exercise Science 16(3): 1-11, 2023. Lifestyle interventions focusing on prenatal physical activity (PA) and healthy nutritional habits can carry forward into the postpartum period. As many health resources, like PA facilities and postpartum support groups, were inaccessible due to the Coronavirus-19 (COVID-19) pandemic restrictions, it may be plausible that individuals who participated in a prenatal lifestyle intervention continued engaging in positive health behaviours on their own. This study explored experiences of postpartum individuals during the pandemic who had engaged in a prenatal PA and nutrition program prior to COVID-19. Semi-structured interviews were completed with postpartum individuals following …

Teloportwrapper: A New Tool For Understanding The Dynamic World Of Fungal Telomere Ends, Trey Stansfield

Mahurin Honors College Capstone Experience/Thesis Projects

Telomeres are repetitive DNA sequence motifs found at eukaryote chromosome ends. Telomeres help protect chromosome ends from DNA damage and promote chromosome stability. Chromosomes play important roles in aging, mutation, and cancer. Eukaryotic pathogens also use telomeres to mutate and manage virulence genes. In response to chromosome end breakage newly formed telomeres, called de novo telomeres, are formed to recreate the lost telomere and sub-telomeric regions.

Magnaporthe oryzae is a fungal pathogen which causes wheat blast, a deadly plant disease in wheat. Magnaporthe oryzae is also known for its highly variable sub-regions which show high amounts of induced variability due …

Evaluation Of Ionic Liquid Treatment As An Alternative To Chemical Fixation And Dehydration In The Preparation Of Biofilms For Scanning Electron Microscopy, Molly Shreve

Mahurin Honors College Capstone Experience/Thesis Projects

Scanning electron microscopes (SEM) give researchers the ability to observe very small features on biological specimens at the nanometer scale, which is a much higher resolution than what can be obtained with a light microscope. To be observed under SEM, unlike light microscopes, samples must be dry, conductive, and able to withstand electron bombardment. Preparation methods have been developed that work well for samples composed mainly of proteins and lipids, but these fail for samples that are predominantly carbohydrates, such as bacterial biofilms. When carbohydrates of these biofilms are dehydrated, they tend to shrink and collapse, and their normal hydrated …

Growth And Survival Of Amphibians Exposed To Road Salt And Glyphosate-Based Herbicide, Jerica Eaton

Mahurin Honors College Capstone Experience/Thesis Projects

Amphibian populations are threatened by increasing levels of environmental pollution resulting from human activities. Areas with mixed land use that include agriculture and urban development experience increased contamination from surface herbicide and road deicer application. Upon entering water sources, these chemicals have the capacity to disrupt the natural dynamics of aquatic ecosystems. This study aimed to evaluate the effects of these contaminants on amphibian populations. I used the spotted salamander (Ambystoma maculatum) as a model, rearing larvae from hatching to metamorphosis. I subjected larvae in each of the treatment groups to varying levels of herbicide and salt concentrations. …

Novel Application Of Graphene Oxide In Conjunction With Dna Binding Dcas9 For Detecting Antibiotic Resistance Genes, Langley Williams

Mahurin Honors College Capstone Experience/Thesis Projects

The frequent emergence of antibiotic resistance genes poses a stumbling block to prompt treatment of pathogen-originating diseases and therefore spurs the demand for a simple, rapid diagnostic device that can outcompete the time and cost of traditional methods such as PCR and cell culturing. The proposed method for detecting pathogenic DNA utilizes the binding properties of deactivated CRISPR-associated protein9 (dCas9) complexed with fluorescein-labeled single-guide RNA (sgRNA) and the fluorescence quenching properties previously demonstrated in graphene oxide (GO). For our assay, we designed sgRNAs to target specific 20 nucleotide sequences extracted from the tetracycline resistance (tetM) gene sequence and covalently modified …

Ua37/46 Charles Taylor Faculty/Staff Personal Papers, Wku Archives

WKU Archives Collection Inventories

Personal papers of Charles Taylor.

Analysis Of A Cathepsin Protease In Normal Development And Tumor Metastasis In Drosophila Melanogaster, Lindsey Fannin

Mahurin Honors College Capstone Experience/Thesis Projects

Cysteine cathepsins are an important class of fifteen proteases with involvement in normal development and tumor progression. To better understand their contribution to these processes, the cathepsin L protease in Drosophila melanogaster was studied. In part one of this project, the role of this cathepsin in the normal development of Drosophila wings was analyzed. The GAL4/UAS-system and RNAi were used to downregulate this cathepsin in various regions of the wing and progeny were inspected for phenotypic irregularities. An abnormal phenotype of curved wings was found and hypothesized to be a result of apoptosis. Further experimentation will be required to prove …

Bibliography, Bruce A. Schulte

Faculty/Staff Personal Papers

Bibliography of publications by Bruce Schulte.

Ua1c11/124 Charles Taylor Photo Collection, Wku Archives

WKU Archives Collection Inventories

Photographs removed from Charles Taylor's personal papers.

Comparison Of Groundwater Chemistry And Associated Biodiversity Of Sulfidic Karst Habitats In Southcentral Kentucky, Kendall Wheeler

Mahurin Honors College Capstone Experience/Thesis Projects

In most ecosystems, primary production is conducted by plants or photosynthetic microbes; however, extremophilic sulfur-oxidizing bacteria can use hydrogen sulfide gas (H2S) as an electron donor to produce metabolic energy in a type of metabolism called chemoautotrophy. These ecosystems are a prominent feature of some karst landscapes, and two have been observed in the Mammoth Cave system, Sulphur River in Parker Cave and Marianne’s Pass in Mammoth Cave National Park respectively.

Sulfidic water is ubiquitous in southcentral Kentucky and is strongly associated with hydrocarbon deposits. This oil boom of the 1880s in this region predates most environmental regulation and records …

Bibliography, Maire M. Blankenship

Faculty/Staff Personal Papers

Bibliography of publications by Maire Blankenship.

The Systems Measurement Of Mammalian Biotas, Part Two, Charles H. Smith, Patrick Georges, Ngoc Nguyen

Faculty/Staff Personal Papers

For a recent publication, the authors identified a seven-region model of mammal family distribution patterns, in which each unit contributes equally to the system’s overall statistical characteristics of diversity, despite its individual units having measurably different levels of diversity and endemism. This systemization presents a highly efficient descriptive model that can possibly be interpreted as a form of natural classification. An additional analysis of the same mode is described here, in which the seven-region model of the distribution of mammal families’ spatial affinities is shown to closely approach a most-probable-state arrangement, as assessed through combinatorics, raising some important questions about …

A Systematic Review Of The Effects Of Meditative And Mindful Walking On Mental And Cardiovascular Health, Dustin W. Davis, Bryson Carrier, Kyle Cruz, Brenna Barrios, Merrill Russen Landers, James W. Navalta

International Journal of Exercise Science

International Journal of Exercise Science 15(2): 1692-1734, 2022. Meditative and mindful exercise are types of physical exercise during which people pay attention, on purpose, to each new present moment without judging their experience. The goal is to apply an accepting awareness of the environment, bodily sensations, thoughts, and emotions without labeling them (e.g., good or bad). The literature centers on qigong, tai chi, and yoga, which are types of mindful exercise that improve mental and cardiovascular health. It is unclear if meditative and mindful walking also improve these health domains. To the authors’ knowledge, this question has not been addressed …

Physical Activity Independently Predicts Perceived Stress During The Covid-19 Pandemic In Private University Students, Curtis W. Brownell, Laura S. Kabiri, Cassandra S. Diep, Heidi Y. Perkins, Amanda M. Perkins-Ball, Augusto X. Rodriguez

International Journal of Exercise Science

International Journal of Exercise Science 15(7): 1680-1691, 2022. Physical activity has significantly declined during the COVID-19 pandemic. Declines in physical activity have correlated with increased levels of perceived stress, though studies examining physical activity and stress have failed to account for critical confounds. The present study aims to determine whether physical activity independently predicts perceived stress in students attending private four-year universities. Physical activity, socioeconomic status, resilience, gender, and perceived stress data were collected from 85 students and used in a multiple linear regression analysis. The regression model accounted for 43.5% of the variance in perceived stress (R2 = .462, …

Muscle Architecture And Strength Changes Induced By Different Resistance Training Frequencies And Detraining, Tiago Volpi Braz, Felipe Brigatto, Danilo Rodrigues Batista, Wellington Gonçalves Dias, Luan Oenning Col, Guilherme Borsetti Businari, Jhenipher Rosolem, Julio Benvenutti Bueno De Camargo, Paulo Henrique Barbosa, Charles Ricardo Lopes

International Journal of Exercise Science

International Journal of Exercise Science 15(4): 1661-1679, 2022. The purpose of the present study was to investigate muscle thickness and strength outcomes of the quadriceps femoris induced by different resistance training (RT) frequencies and detraining. In addition, muscle architecture (MA) parameters were also assessed. Twenty-seven healthy resistance-trained subjects (men, n = 17; women, n = 10; 20.8 ± 1.9 years; RT experience = 3.3 ± 1.6 years) volunteered to participate in this study. One leg of each subject was randomly allocated into the 2 sessions per week condition (2x) and the contralateral leg was then placed in the 4 sessions …

A Preliminary Comparison Of Firefighter Candidates' Biddle Physical Ability Test Performance And Success Based On Training Class Participation, Robert G. Lockie, Tomas Ruvalcaba, Megan Thompson, Erika Viramontes, Robin (Rob) Marc Orr, J. Jay Dawes, Joseph Dulla

International Journal of Exercise Science

International Journal of Exercise Science 15(4): 1627-1640, 2022. The Biddle Physical Ability Test (BPAT) was developed to identify candidates who possess the physical ability to become structural firefighters. The test must be completed in ≤ 9:34 min:s before a candidate is admitted to an academy. Some community colleges offer semester-long training classes for candidates. This study analyzed whether candidates who completed a training class could perform the BPAT more effectively. Retrospective analysis of 30 males and 2 females who attempted the BPAT was conducted. BPAT tasks were: dry and charged hose drag; halyard raise, roof walk, and attic crawl; roof …

The Effects Of Traditional Versus Ability-Based Physical Training On The Health And Fitness Of Custody Assistant Recruits, Karly Rodas, Joseph Dulla, Matthew R. Moreno, Ashley Bloodgood, Megan Mcguire, Robin (Rob) Marc Orr, J. Jay Dawes, Robert G. Lockie

International Journal of Exercise Science

International Journal of Exercise Science 15(3): 1641-1660, 2022. This study investigated traditional training (TT) versus ability-based training (ABT) in custody assistant recruits. Retrospective analysis was conducted on two recruit classes who completed an 8-week academy. The TT group (18 males, 13 females) followed a physical training model where recruits completed the same exercises with the same intensity; the ABT group (17 males, 12 females) had exercises tailored towards their ability. Pre- and post-academy, recruits were assessed in: body mass; body fat percentage (BF%); resting heart rate (RHR); blood pressure (BP); waist circumference (WC); waist-to-hip ratio (WHR); grip strength; push-ups; sit-ups; …

Near Infrared Spectroscopy Is Not A Surrogate Of Venous Occlusion Plethysmography To Assess Microvascular Resting Blood Flow And Function, Manuel Gomez, Alvaro N. Gurovich, Samuel Montalvo

International Journal of Exercise Science

International Journal of Exercise Science 15(2): 1616-1626, 2022. Near-infrared spectroscopy (NIRS) is a non-invasive technique that measures tissue perfusion using red blood cells oxygen saturation and venous occlusion plethysmography (VOP) is the gold standard to assess microvascular blood flow and function. The purpose of this study was to determine if NIRS can surrogate the microvascular blood flow assessment after an ischemic challenge obtained via VOP. Twenty apparently healthy subjects (10 males and 10 females), aged 18 to 35 years, were recruited for this single session study. NIRS probes were placed 40mm apart along the epicondylar muscles on the right forearm …

Factors Influencing Motivation To Perform Mental And Physical Tasks During The Initial Lockdown Period Of The Covid-19 Pandemic, Niklas Mckone, Joel R. Martin, Italia Milani, Shantanu Sur, Sumona Mondal, Nelson Cortes, Shane V. Caswell, Ali Boolani

International Journal of Exercise Science

International Journal of Exercise Science 15(5): 1600-1615, 2022. Drastic changes to lifestyles have occurred during the COVID-19 pandemic. An unintended consequence of stay at home orders is increased isolation and less social interaction for many people. For overall wellbeing it is important to stay both physically and mentally active; however, for many individual’s motivation may be a barrier. There are non-modifiable (e.g. sex, age, personality, infection rates in the area) and modifiable factors (e.g. physical activity, diet, sleep) that may be associated with motivation to perform physical and mental tasks. We collected data from 794 subjects using an online survey …

Effect Of Real-Time Feedback On Power Output Using A Novel Smart-Resisted Sled Push, Phillip Goldman, Bilal Pandit, David Gomez, Stevin Lu, Cade Mills, Nick Kull, Richard Ku, Adrian Aramie, Alex Kim, Anka Alexdandru, Jonathan Hu, Eric Neufeld, Brett Dolezal

International Journal of Exercise Science

International Journal of Exercise Science 15(6): 1578-1586, 2022. Prior studies have demonstrated the beneficial effects of real-time data feedback (RTF) on athletic performance and motivation. Despite this evidence, the lack of practical means to implement RTF has hindered its widespread adoption. Recently, a smart-resisted sled push was developed to improve athletic power by utilizing electromagnetic motors as a resistance mechanism, coupled with an RTF display. Thirty healthy college-aged male football players were recruited in this randomized, crossover designed study to examine the efficacy of the RTF to improve power output. Participants were randomized into either group 1 (receiving RTF first …

Longitudinal Changes In Fat And Lean Mass: Comparisons Between 3d-Infrared And Dual-Energy X-Ray Absorptiometry Scans In Athletes, Matthew Vansumeren, Spencer Weber, Justin Civelek, Jordan Sabourin, Valerie Smith-Hale, Tamara Hew-Butler

International Journal of Exercise Science

International Journal of Exercise Science 15(4): 1587-1599, 2022. The low cost and portability of three-dimensional (3D) infrared body scanners make them an attractive tool for body composition measurement in athletes. The main purpose of this study was to compare total body fat percentage (BF%) and total lean mass (LM in kg), in a cohort of collegiate athletes, using a 3D infrared body scanner versus a dual energy x-ray absorptiometry (DXA) scanner. Phase I was a pre-season cross-sectional analysis of 61 (39 male) athletes while Phase II was a longitudinal subset analysis of 38 (27 male) student-athletes who returned to the …