Life Sciences Commons^™

Two Genetic Effects At The Irf5/ Tnpo3 Locus Are Independently Associated With The Development Of Specific Lupus Symptoms, Samantha Hawtrey

Mahurin Honors College Capstone Experience/Thesis Projects

Systemic Lupus Erythematosus (SLE) is an autoimmune disorder that can affect every tissue in the body. Single nucleotide polymorphisms (SNPs) in the IRF5 and TNPO3 genes are statistically associated with the development of SLE. My research identified correlations between IRF5/TNPO3 SNPs and specific lupus symptoms. Logistic regression analyses were conducted using 101 genetic variants in the IRF5/TNPO3 region that were genotyped in over 6,000 lupus patients of different ethnicities, with admixture covariates applied. Three clinical phenotypes displayed significant correlation (p < 1.6x10-5) in subjects of European ancestry. For each of these phenotypes, a step-wise conditional analysis was conducted using two lupus associated single nucleotide polymorphisms (SNPs) at this genetic loci. In Europeans, lupus disease onset (p-valueEU=2.44x10-16, OR=0.67*) and the presence of anti-Ro (p-valueEU=2.09x10-7, OR=0.67) and anti-dsDNA (p-valueEU=4.15x10-7, OR=0.75) antibodies were associated with SNPs in the IRF5/TNPO3 genes. SNPs in the IRF5 promoter and those spanning IRF5 and TNPO3 were both associated with disease onset. The presence of anti-Ro and anti-dsDNA antibodies is only associated with SNPs in the IRF5 promoter. Genetic variants at the IRF5/TNPO3 locus are associated with lupus disease onset and production of anti-dsDNA and anti-Ro antibodies in lupus patients. SNPs in the promoter region of iii IRF5 (associated with rs4728142) and SNPs spanning the IRF5 and TNPO3 genes (associated with rs12534421) contribute independently to these symptoms.

Population Genetics Of The Western Toad (Bufo Boreas) In The Central Valley Of California, Morgan Murrell

Mahurin Honors College Capstone Experience/Thesis Projects

A worldwide decline in amphibian populations has intensified the need for data comparing the influence of habitats on population dynamics and the potential for local extinction. From a conservation perspective it is important to understand the connections between ecology, geography, and genetics across landscapes that are increasingly affected by human influences and other uncontrollable environmental events such as climate change. The purpose of this study is to examine the landscape-level genetic patterns of Western toads, Bufo (Anaxyrus) boreas, and to conclude if gene flow is occurring between ponds. This will allow conservation practitioners to understand geographic features that might impede …

Ogden College Of Science & Engineering Newsletter (Winter 2013), Cheryl Stevens, Dean

Ogden College of Science & Engineering Publications

No abstract provided.

Novel Applications Of Multivariate Methods For Exploring Personality In African Elephants, Shilo Kimberly Felton

Masters Theses & Specialist Projects

Investigators have shown that elephants exhibit consistent individual differences in behavior by rating elephants using personality adjectives. These adjectives, however, are not based on pre-defined measurements of the behaviors performed. Instead, they are based on the observers’ interpretations of an animal’s behavioral patterns, therefore making them subject to observer bias. Furthermore, elephants have a capacity for learning; thus, they may alter their behavioral patterns over time. This behavioral plasticity in itself might be a way of measuring consistent behavioral differences among individuals. With this in mind, I approached elephant personality as a multivariate problem. I used behavioral observations collected from …

Landscape Genetics Of The Marbled Salamander (Ambystoma Opacum) At Mammoth Cave National Park, James Kyle Martin

Masters Theses & Specialist Projects

Habitat connectivity is important to maintain in order to prevent loss of genetic diversity, reduce inbreeding depression, and decrease extinction risk in threatened or endangered species. Here I present a landscape genetics study on marbled salamanders (Ambystoma opacum) in highly connected forested habitat at Mammoth Cave National Park. This investigation of gene flow among ponds within a mostly continuous landscape provides data that can be compared with patterns observed in more fragmented landscapes. These comparisons can provide a means of investigating the separate effects of structural and functional habitat connectivity on amphibian genetic population structure. Structural connectivity refers to the …

Single Nucleotide Polymorphisms Linked To Essential Hypertension In Kasigau, Kenya, Julia Carol Freeman

Masters Theses & Specialist Projects

Hypertension, or high blood pressure (BP), is an ever-growing epidemic in the developing world. Understanding the genetics behind essential hypertension (EH), or hypertension with no known cause, is especially important. In this study, three single nucleotide polymorphisms (SNPs) known to be linked to an increase in susceptibility to EH were quantified from a cohort of Kenyans living in the Kasigau region. The SNPs are located in three genes that are part of the renin angiotensin system, the primary regulatory pathway in humans controlling BP. They include: AGT (rs699), AGTR1 (rs5186), and HSD11β2 (rs5479). Overall, by using a fluorescent-based RT-PCR technique, …

Interference In White Bass Reproduction By Two Introduced Predators In Barren River Lake, Kentucky, Jacob Franklin Fose

Masters Theses & Specialist Projects

White bass Morone chrysops are native to the Mississippi River and its tributaries. This range includes the Barren River in south central Kentucky. Over the last thirty years, the population of white bass in Barren River Lake, a reservoir of the Barren River, has been in decline. During that same time, two congeners of white bass have been introduced to the lake. Hybrid striped bass Morone chrysops × Morone saxatilis were introduced in 1979 and yellow bass Morone mississippiensis were first discovered in 2000. Due to the similar life histories and spawning strategies of all three Morone species, I hypothesized …

Wallace And Incipient Structures: A World Of "More Recondite" Influences, Charles H. Smith

DLPS Faculty Publications

Alfred Russel Wallace is well-known for his co-discovery of the principle of natural selection. Natural selection is usually considered a process, but it is not clear that Wallace regarded it in exactly these terms. In fact he more likely thought of the relationships involved as representing what we would now term a “state space,” a negative feedback loop wherein populations are maintained at healthy levels through elimination of the unfit. Both before and after the advent of natural selection Wallace clung to the idea that “more recondite forces” were shaping the nature and direction of evolution; this is especially evident …

Fluttering Beauty, Jonathan Jeffrey

SCL Faculty and Staff Publications

This article chronicles the efforts of Lillian Iona (Tyne) Pace (1925-2010) in getting the Viceroy butterfly designated as the Kentucky state butterfly.

Kentucky Warbler (Vol. 89, No. 4), Kentucky Library Research Collections

Kentucky Warbler

No abstract provided.

Carbohydrate Mouth Rinse Improves 1.5 H Run Performance: Is There A Dose-Effect?, Benjamin F. Wright, Glen Davison

International Journal of Exercise Science

International Journal of Exercise Science 6(4) : 328-340, 2013. There is a substantial body of recent evidence showing ergogenic effects of carbohydrate (CHO) mouth rinsing on endurance performance. However, there is a lack of research on the dose-effect and the aim of this study was to investigate the effect of two different concentrations (6% and 12% weight/volume, w/v) on 90 minute treadmill running performance. Seven active males took part in one familiarization trial and three experimental trials (90-minute self-paced performance trials). Solutions (placebo, 6% or 12% CHO-electrolyte solution, CHO-E) were rinsed in the mouth at the beginning, and at 15, …

The Influence Of Start Position, Initial Step Type, And Usage Of A Focal Point On Sprinting Performance, Jennifer L. Dysterheft, William J. Lewinski, Dawn A. Seefeldt, Robert W. Pettitt

International Journal of Exercise Science

International Journal of Exercise Science 6(4) : 320-327, 2013. For many athletes, sprinting acceleration is vital to sport performance. The purpose of this study was to observe the influences of starting position, type of initial step taken, and a focal point on sprinting velocity, stride length, and acceleration over a 9.1 m distance. Two trials of four conditions were video recorded in which subjects had no focal point (n = 10) or a lateral focal point (n ₌ 9). The four conditions were: forwards (control), backwards, 90° left (90°L), and 90° right (90°R). Lower velocities (p > 0.05) were …

Evaluating Acute Changes In Joint Range-Of-Motion Using Self-Myofascial Release, Postural Alignment Exercises, And Static Stretches, Derek S. Roylance, James D. George, Adam M. Hammer, Nicole Rencher, Gilbert W. Fellingham, Ronald L. Hager, William J. Myrer

International Journal of Exercise Science

International Journal of Exercise Science 6(4) : 310-319, 2013. This study was designed to compare the acute effect of self-myofascial release (SMR), postural alignment exercises, and static stretching on joint range-of-motion. Our sample included 27 participants (n = 14 males and n = 13 females) who had below average joint range-of-motion (specifically a sit-and-reach score of 13.5 inches [34.3 cm] or less). All were university students 18–27 years randomly assigned to complete two 30–40-minute data collection sessions with each testing session consisting of three sit-and-reach measurements (which involved lumbar spinal flexion, hip flexion, knee extension, and ankle dorsiflexion) interspersed with …

Increasing The Distance Of An External Focus Of Attention Has Limited Effects On Standing Long Jump Performance, William Westphal, Jared M. Porter

International Journal of Exercise Science

International Journal of Exercise Science 6(4) : 300-309, 2013. Several previous studied have demonstrated that adopting an external focus of attention (i.e., directing attention towards the result of the movement) improves the performance of a variety of motor skills. The objective of this study was to investigate if increasing the distance of an external focus of attention would further improve standing long jump performance. We hypothesized that as the distance of the external focus increased so would jumping distance. We also hypothesized that when subjects completed jumps in the external focus of attention conditions they would jump significantly further than …

Biomechanical And Proprioceptive Differences During Drop Landings Between Dancers And Non-Dancers, Katie E. Volkerding, Caroline J. Ketcham Ph.D.

International Journal of Exercise Science

International Journal of Exercise Science 6(4) : 289-299, 2013. The focus of this research was to determine if female dancers have differing kinematic and kinetic characteristics when landing from three heights (0.2, 0.5, and 0.8 m) both with and without vision compared to non-dancers. It was hypothesized that dancers would show differing kinematic and kinetic patterns of control due to their increased proprioceptive awareness. Eight collegiate dancers and seven collegiate controls who were neither dancers nor collegiate jumping athletes volunteered for this study. Sagittal plane lower limb joint angles were measured at 100 Hz prior to landing through stability with …

Upper Body Muscular Activation During Variations Of Push-Ups In Healthy Men, Caroline C. Allen, Katie A. Dean, Alan P. Jung, John K. Petrella

International Journal of Exercise Science

International Journal of Exercise Science 6(4) : 278-288, 2013. The purpose of this study was to assess neural activity for upper body musculature in college-age men during repetitions of a conventional pushup or a Perfect Pushup^TM. Eighteen healthy men (21.6±1 yr, 182.5±7 cm, 87.4±15 kg) completed five repetitions of a conventional pushup and Perfect Pushup^TM while using a wide hand base of support for the upper body. Body position, hand placement, and cadence of the pushup were standardized. Root mean square electromyography (RMS-EMG, mV/Sec) was collected for the triceps brachii (TB), pectoralis major (PM), serratus anterior (SA), …

Comparisons Between Swing Phase Characteristics Of Race Walkers And Distance Runners, Laura C. Smith, Brian Hanley

International Journal of Exercise Science

International Journal of Exercise Science 6(4) : 269-277, 2013. The aim of this study was to analyze swing characteristics during race walking and to compare these with distance running. The rules of race walking demand that no visible flight time should occur and the stance leg must be straightened from initial contact to midstance. Previous research has not examined whether these rules also have an effect on swing and what consequences might arise. Ten male race walkers and ten male distance runners walked or ran respectively on an instrumented treadmill for 10 km with two in-dwelling force plates. Trials lasted …

Attitudes Toward And Preferences For Male And Female Personal Trainers, James P. Fisher, Catherine Platts, Marie Stopforth

International Journal of Exercise Science

International Journal of Exercise Science 6(4) : 256-268, 2013. Previous research has considered the perceptions of athletes towards gender of coach and strength and conditioning coach. However, to date there appears little research considering the perceptions of clients towards gender of personal trainer. The purpose of this study was to investigate male and females perceptions of same- or opposite-gender personal trainers. Four hundred and two (male = 201, female = 201) undergraduate University student participants completed an adapted version of the Attitudes of Athletes toward Male versus Female Coaches Questionnaire (AAMFC-Q; 23). A 2 x 2 multivariate analysis of variance …

Early Humboldtian Influences On Alfred Russel Wallace's Scheme Of Nature [Presented At The Alfred Russel Wallace And His Legacy Royal Society Of London Meeting, 21 October 2013], Charles H. Smith

DLPS Faculty Publications

Alfred Russel Wallace’s 1858 Ternate paper on natural selection is a famous work in the history of science. Beyond his co-discovery of the principle, moreover, Wallace is known for a large number of early applications of the idea, both to biological and biogeographical subjects. Yet how much do we really know about Wallace’s own evolution of thought, and his actual intentions before his views were swallowed up by the inertia of Darwin’s revolution? A number of differences between Wallace’s and Darwin’s views are apparent and have been much treated over the years, but related discussions dwell more on effects than …

Moore, Carlos Wright, 1884-1951 (Mss 156), Manuscripts & Folklife Archives

MSS Finding Aids

Finding aid only for Manuscripts Collection 156. Family and business papers of Warren County, Kentucky farmer Carlos W. Moore and school teacher Mae White, who became his wife. Topics include courtship, farming and school teaching.

Borders, Forest, 1893-1971 (Sc 1173), Manuscripts & Folklife Archives

MSS Finding Aids

Finding aid only for Manuscripts Small Collection 1173. Miscellaneous materials from Forest Borders, including newspaper clippings, magazine articles, and advertising related to his Borders Pure Milk Company of Bowling Green, Kentucky. Also includes a soil conservation plan with maps for his Warren County farm.

Comparisons Of Genetic Diversity Among Disjunct Populations Of Magnolia Tripetala, Victoria A. Gilkison

Mahurin Honors College Capstone Experience/Thesis Projects

Plant ranges are often made up of core areas where the distribution is continuous and the population density is high with small disjunct populations at the margins of the core. One well-studied type of disjunct population is formed by long distance dispersal as plants migrate away from disjunct Pleistocene refugial populations. The retreat of the Wisconsinan glaciation resulted in the outward dispersal of many plant species from their refugial locations to areas with suitable habitat. Many plants expanded their ranges through rare-long-distance dispersal.

This study used microsatellites to compare the genetic diversity, inbreeding levels, and gene flow frequency of disjunct …

The Creation Of Α2nc1 Transgenic Drosophila Melanogaster, Jordan Olberding

Mahurin Honors College Capstone Experience/Thesis Projects

The α2 isoform of collagen IV’s noncollagenous 1 domain (α2NC1 or canstatin) has shown promise as an anti-cancer molecule, possessing both angiostatic and pro-apoptotic properties. This work aims to further the knowledge of α2NC1 by conducting a truly in vivo study in which the Gal4-induced endogenous overexpression of α2NC1 in Drosophila melanogaster will be used to assess the effects of α2NC1 upon normal development as well as tumorigenesis. To this end, transgenic fly lines capable of overexpressing α2NC1 were created. Initial overexpression studies indicated no developmental phenotype was caused by abundance of α2NC1. However, future overexpression studies in which α2NC1 …

Defining The Requirements For Early Gene Expression In Bacteriophage Hk639, Amanda L. Seaton

Masters Theses & Specialist Projects

Lambdoid phages suppress transcription termination to fully express their genes. Antitermination of early gene expression in most lambdoid phages is mediated by an interaction between the N protein and a number of host-encoded factors. Bacteriophage HK022 does not rely on a protein for antitermination. To promote full expression of early phage genes, the transcripts of the HK022 put sites interact directly with RNA polymerase to convert it to a termination resistant form. Bacteriophage HK639 also uses RNA-mediated antitermination. However, it only possesses a single put-like element in its left operon. Because most lambdoid phages, including HK022, have antiterminator elements in …

Kentucky Warbler (Vol. 89, No. 3), Kentucky Library Research Collections

Kentucky Warbler

No abstract provided.

Cultivar, Mowing Height, And Herbicide Effects On Bermudagrass, Cynodon Dactylon [L.] Pers., Suppression In Tall Fescue, Schedonorus Arundinaceus [Schreb.] Dumort., Nom. Cons., Daniel S. Sandor

Masters Theses & Specialist Projects

In the fall of 2011, a study was initiated at the Western Kentucky University Farm in Bowling Green, Kentucky on a Crider silt loam (Typic Paleudalf). The objective of this study was to determine cultivar, mowing height, and herbicide effects on bermudagrass, Cynodon dactylon [L.] Pers., suppression in tall fescue, Schedonorus arundinaceus [Schreb.] Dumort., nom. cons. The experimental design was a split plot design with whole plots consisting of varying mowing heights and split plots consisting of different herbicide treatments with three replications. Two separate experiments were conducted. The tall fescue variety ‘KY 31’ was utilized for one study and …

Single Step Synthesis Of Antibiotic Kanamycin Embedded Gold Nanoparticles For Efficient Antibacterial Activity, Shravan Gavva

Masters Theses & Specialist Projects

Nanotechnology has become the most advanced type of drug delivery system within the last decade. This advancement shifted the focus on small carriers to increase the efficiency of the drugs. Among these, gold nanoparticles (GNPs) were found to have profound biomedical applications. In current research, kanamycin embedded GNPs were prepared in a single step, single phase, and bio-friendly (green synthesis) procedure. The synthesized Kanamycin-GNPs (Kan-GNPs) were spherical in shape and had a size range of 15 ± 3 nm. The chosen kanamycin is an aminoglycosidic antibiotic that is isolated from Streptomyces kanamyceticus. These special antibiotic GNPs are further characterized using …

Development Of Tools To Assess The Effects Of Lunasin On Normal Development And Tumor Progression In Drosophila Melanogaster, Gillian E. Jones

Masters Theses & Specialist Projects

Soy contains many bioactive molecules known to elicit anti-cancer effects. One such peptide, Lunasin, has been shown to selectively act on newly transformed cells while having no cytotoxic effect on non-tumorigenic or established cancer cell lines. In this study we attempt to understand the developmental effects of Lunasin overexpression in vivo and create reagents that will help us understand Lunasin’s anti tumorigenic effects in an intact organism. cDNA encoding lunasin and EGFP-lunasin were cloned into pUAST and microinjected into Drosophila embryos. Tissue-specific overexpression of EGFP-Lun in the resulting transgenic lines was accomplished by crossing transgenics to various GAL4 driver lines. …

Effects Of Xanthan/Locust Bean Gum Mixtures On The Physicochemical Properties And Oxidative Stability Of Whey Protein Stabilized Oil-In-Water Emulsions, Goutham Puli

Masters Theses & Specialist Projects

Scientific evidence shows that dietary intake of the omega-3 polyunsaturated fatty acids is beneficial to human health. Fish oil is a rich source of omega-3 fatty acids. However, fish oil with high levels of omega-3 PUFA is very susceptible to oxidative deterioration during storage. The objective of this study was to investigate the effect of xanthan gum (XG)-locust bean gum (LBG) mixtures on the physicochemical properties of whey protein isolate (WPI) stabilized oil-in-water (O/W) emulsions containing 20% v/v menhaden oil. The O/W emulsions containing XG/LBG mixtures were compared to emulsions with either XG or LBG alone. The emulsions were prepared …

Acquisition Delays Affect Lymphocyte Subset Counts But Not Markers Of Exercise-Induced Apoptosis, Holly B. Kell, Guilherme Borges Pereira, Ramires Alsamir Tibana, Michael Jarrett, Mark A. Schafer, Jonato Prestes, Antonio Santo, James W. Navalta

International Journal of Exercise Science

International Journal of Exercise Science 6(3) : 250-255, 2013. With the emergence of shared facilities, the possibility of a processing delay is increased as time on instrumentation becomes less available. The purpose of this investigation was the evaluate the effect of a 24h time delay on lymphocyte subset concentration, as well as for the apoptotic marker annexin V. Fourteen healthy individuals completed an incremental treadmill test to exhaustion, and blood samples were obtained before and after exercise. The samples were labeled in duplicate with mixtures consisting of flow cytometry staining buffer, the biomarker for early-phase cell death (annexin V), and …