Life Sciences Commons^™

The Ban On The Use Of Chimpanzees In Biomedical Research And Testing In The Uk Should Be Made Permanent And Legally Binding, Michelle Thew, Jarrod Bailey, Michael Balls, Michelle Hudson

Laboratory Experiments Collection

The Coalition Government is currently considering how to transpose Directive 2010/63/EU on animal experimentation into UK law. The Directive bans the use of Great Apes in laboratories, but EU Member States can seek (now or, more likely, at some time in the future) a derogation from the Commission to permit such use, where this is considered essential for the preservation of the species in question or in relation to an unexpected outbreak of a life-threatening or debilitating clinical condition in human beings. Currently, the policy of the Government is not to approve any experiments on Great Apes, but it is …

Alternative Approaches For Medical Countermeasures To Biological And Chemical Terrorism And Warfare, Thomas Hartung, Joanne Zurlo

Biomedical Research and Alternative Methods Collection

The desire to develop and evaluate drugs as potential countermeasures for biological and chemical threats requires test systems that can also substitute for the clinical trials normally crucial for drug development. Animal models have limited predictivity for drug efficacy, as is well known from many disappointments in clinical trials. Traditional in vitro and in silico approaches are not really game changers here, but the substantial investment into novel tools now underway might bring about a second generation of alternative approaches. The avenue pursued focuses primarily on the development of a Human on a Chip, i.e., the combination of different three-dimensional …

Isotopic Niche Differentiation Among Mammals From A Rainforest In Peninsular Malaysia, Kae Kawanishi, Song Horng Neo Liang, Chris T. Darimont, Thomas E. Reimchen, Melvin E. Sunquist

Biochemistry Collection

We performed stable isotope analysis on eight mammalian species: pig-tailed macaque (Macaca nemestrina), long-tailed macaque (M. fascicularis), dusky leaf monkey (Trachypithecus obscurus), brush-tailed porcupine (Atherurus macrourus), wild boar (Sus scrofa), lesser mouse-deer (Tragulus javanicus), greater mouse-deer (T. napu), and barking deer (Muntiacus muntjac), to test the hypothesis that the differences in diet and habitat types among species, guilds and foraging strategies are reflected in the δ¹⁵N and δ¹³C signatures of their tissues. Whereas the isotopic ratios differed among taxa, the four major isotopic groups observed were: mouse-deer species, primate species, brush-tailed porcupine, and wild boar. The …

Bias During The Evaluation Of Animal Studies, Andrew Knight

Morality and Ethics of Animal Experimentation Collection

My recent book entitled The Costs and Benefits of Animal Experiments seeks to answer a key question within animal ethics, namely: is animal experimentation ethically justifiable? Or, more precisely, is it justifiable within the utilitarian cost:benefit framework that fundamentally underpins most regulations governing animal experimentation? To answer this question I reviewed more than 500 scientific publications describing animal studies, animal welfare impacts, and alternative research, toxicity testing and educational methodologies. To minimise bias I focused primarily on large-scale systematic reviews that had examined the human clinical and toxicological utility of animal studies. Despite this, Dr. Susanne Prankel recently reviewed my …

Whaling: Don’T Trade The Moratorium Away, Mark Peter Simmonds, Sue Fisher

Conservation Collection

In their proposal to allocate ‘whale shares’ to both whalers and conservationists as an alternative to the International Whaling Commission (IWC) moratorium on commercial whaling, Christopher Costello and colleagues overlook several factors (Nature 481, 139–140; 2012).

Complex Patterns Of Male Alliance Formation In A Dolphin Social Network, Joanna Wiszniewski, Culum Brown, Luciana M. Möller

Sentience Collection

The formation and maintenance of alliances is regarded as one of the most socially complex male mating strategies in mammals. The prevalence and complexity of these cooperative relationships, however, varies considerably among species as well as within and between populations living in different ecological and social environments. We assessed patterns of alliance formation for Indo-Pacific bottlenose dolphins, Tursiops aduncus, in Port Stephens, New South Wales, Australia, to investigate the stability of these alliances, the structure of associations, as well as variation in schooling patterns among males. Our results showed that association patterns among males within this population showed considerable variability. …

Superglue Is Not Super: An Assessment Of Superglue For Suturing Tag Incisions In A Cultured Marine Fish, Vincent Raoult, Culum Brown, Jane E. Williamson

Aquaculture Collection

No abstract provided.

Accelerating The Development Of 21st-Century Toxicology: Outcome Of A Human Toxicology Project Consortium Workshop, Martin L. Stephens, Craig Barrow, Melvin E. Andersen, Kim Boekelheide, Paul L. Carmichael, Michael P. Holsapple, Mark Lafranconi

in Vitro Research Models Collection

The U.S. National Research Council (NRC) report on “Toxicity Testing in the 21st century” calls for a fundamental shift in the way that chemicals are tested for human health effects and evaluated in risk assessments. The new approach would move toward in vitro methods, typically using human cells in a high-throughput context. The in vitro methods would be designed to detect significant perturbations to “toxicity pathways,” i.e., key biological pathways that, when sufficiently perturbed, lead to adverse health outcomes. To explore progress on the report’s implementation, the Human Toxicology Project Consortium hosted a workshop on 9–10 November 2010 in Washington, …

An Hsi Report: Industrial Farm Animal Production And Livestock Associated Mrsa (Methicillin Resistant Staphylococcus Aureus), Humane Society International

HSI REPORTS

Staphylococcus aureus is a leading cause of bacterial infection and is increasingly found to be resistant to antibiotic therapy. A newly described type of Methicillin Resistant Staphylococcus aureus carried by farm animals, Livestock Associated MRSA (LA-MRSA), is now causing infections in humans with and without direct livestock contact. A reduction in the non-therapeutic use of antibiotics in feed would likely reduce the capacity of industrial animal agriculture to continue to create, disseminate, and perpetuate a large reservoir of LA-MRSA on a global scale, but more fundamental changes in the way animals are raised for food may be necessary forestall a …

The Fine-Scale Habitat Use Of Risso’S Dolphins Off Bardsey Island, Cardigan Bay (Uk), Marijke N. De Boer, Sonja Eisfeld, Mark P. Simmonds

Ecology Collection

The main objectives of this study were (1) to estimate the population size of Risso’s dolphins off Bardsey Island using mark-recapture techniques (De Boer et al., 2013); and (2) to study habitat-use in relation to fine-scale oceanographic features. This work provides preliminary information on the habitat-use of Risso’s dolphins and will benefit future studies, along with the development of effective conservation measures for this species throughout the region.

Cetaceans And Marine Debris: The Great Unknown, Mark Peter Simmonds

Ecology Collection

Plastics and other marine debris have been found in the gastrointestinal tracts of cetaceans, including instances where large quantities of material have been found that are likely to cause impairment to digestive processes and other examples, where other morbidity and even death have resulted. In some instances, debris may have been ingested as a result of the stranding process and, in others, it may have been ingested when feeding. Those species that are suction or “ram” feeders may be most at risk. There is also evidence of entanglement of cetaceans in marine debris. However, it is usually difficult to distinguish …

Rational Engagement, Emotional Response, And The Prospects For Moral Progress In Animal Use “Debates”, Nathan Nobis

Animal Welfare Collection

This chapter is designed to help people rationally engage moral issues regarding the treatment of animals, specifically in experimentation, research, product testing, and education. Little “new” philosophy is offered here, strictly speaking. New arguments are unnecessary to help make progress in how people think about these issues. What is needed are improved abilities to engage the arguments already on the table, for example, stronger skills at identifying and evaluating the existing reasons given for and against conclusions on the morality of various uses of animals. To help improve these abilities, this chapter sets forth a set of basic but powerful …

Confronting Language, Representation, And Belief: A Limited Defense Of Mental Continuity, Kristin Andrews, Ljiljana Radenovic

Sentience Collection

According to the mental continuity claim (MCC), human mental faculties are physical and beneficial to human survival, so they must have evolved gradually from ancestral forms and we should expect to see their precursors across species. Materialism of mind coupled with Darwin’s evolutionary theory leads directly to such claims and even today arguments for animal mental properties are often presented with the MCC as a premise. However, the MCC has been often challenged among contemporary scholars. It is usually argued that only humans use language and that language as such has no precursors in the animal kingdom. Moreover, language is …

A Queer Vegan Manifesto, Rasmus R. Simonsen

Human Health Collection

What does it mean for a person to declare her or his veganism to the world? How does the transition from one diet to another impact one’s sense of self? Veganism challenges the foundational character of how we “act out” our selves—not least of all in the context of sexuality and gender. In my paper, I am thus interested in the potential of veganism to disrupt the “natural” bond between gender formations and the consumption of animal products, as this relates to social and cultural genealogies. Consequently, I will explore a queer form of veganism that affirms the radical impact …

Clinical Anesthesia And Analgesia In Fish, Lynne U. Sneddon

Veterinary Science and Medicine Collection

Fish have become a popular experimental model and companion animal, and are also farmed and caught for food. Thus, surgical and invasive procedures in this animal group are common, and this review will focus on the anesthesia and analgesia of fish. A variety of anesthetic agents are commonly applied to fish via immersion. Correct dosing can result in effective anesthesia for acute procedures as well as loss of consciousness for surgical interventions. Dose and anesthetic agent vary between species of fish and are further confounded by a variety of physiological parameters (e.g., body weight, physiological stress) as well as environmental …

Characterisation Of Chemosensory Trigeminal Receptors In The Rainbow Trout, Oncorhynchus Mykiss: Responses To Chemical Irritants And Carbon Dioxide, Jessica J. Mettam, Catherine R. Mccrohan, Lynne U. Sneddon

Veterinary Science and Medicine Collection

Trigeminally innervated, mechanically sensitive chemoreceptors (M) were previously identified in rainbow trout, Oncorhynchus mykiss, but it is not known whether these receptors are responsive only to noxious, chemical irritants or have a general chemosensory function. This study aimed to characterise the stimulus–response properties of these receptors in comparison with polymodal nociceptors (P). Both P and M gave similar response profiles to acetic acid concentrations. The electrophysiological properties were similar between the two different afferent types. To determine whether the receptors have a nociceptive function, a range of chemical stimulants was applied to these receptors, including non-noxious stimuli such as ammonium …

Animal Research In Medical Sciences: Seeking A Convergence Of Science, Medicine, And Animal Law, John J. Pippin

Laws and Legislation Collection

As the intersection of animal law and animal research becomes congested, it is appropriate to establish the scientific context in which laws regarding the use and care of research animals will operate. There are at least three components of this context that set the terms of the debate: ethics, science, and the legal status of animals. The following discussion will not address ethics; not because it isn’t important, but because it exists along a spectrum of objective and subjective positions that are often unassailable by argument and data. I can assure you as a former animal researcher that even in …

Pursuing Medawar’S Challenge For Full Replacement, Martin Stephens

Experimentation Collection

In 1969, Nobel Prize-winning scientist Peter Medawar predicted that scientific innovation would someday fully replace the use of animals in biomedical research. Medawar correctly forecast the leveling off and subsequent decline in animal use in the last quarter of the 20th century – a period of remarkable innovation in the life sciences. A 2007 report by the U.S. National Academy of Sciences, Toxicity Testing in the 21st Century, proposed a strategy that is likely to replace all routine animal use in toxicology with innovative methods within one to two decades. Replacing animal use throughout biomedical research is more challenging given …

Prevalence Of Antibodies To Canine Parvovirus And Distemper Virus In Wolves In The Canadian Rocky Mountains, Brynn Nelson, Mark Hebblewhite, Vanessa Ezenwa, Todd Shury, Evelyn H. Merrill, Paul C. Paquet, Fiona Schmiegelow, Dale Selp, Geoff Skinner, Nathan Webb

Epidemiology Collection

Wild carnivores are often exposed to diseases via contact with peridomestic host species that travel through the wildland-urban interfaces. To determine the antibody prevalences and relationships to human activity for two common canid pathogens, we sampled 99 wolves (Canis lupus) from 2000 to 2008 for antibodies to canine parvovirus (CPV) and canine distemper virus (CDV) in Banff and Jasper National Parks and surrounding areas of the Canadian Rockies. This population was the source for wolves reintroduced into the Northern Rockies of the US. Of 99 wolves sampled, 94 had detectable antibody to CPV (95%), 24 were antibody-positive for CDV (24%), …

A Roadmap For The Development Of Alternative (Non-Animal) Methods For Systemic Toxicity Testing, David Basketter, Harvey Clewell, Ian Kimber, Annamaria Rossi, Bas Blaauboer, Robert Burrier, Mardas Daneshian, Chantra Eskes, Alan Goldberg, Nina Hasiwa, Sebastian Hoffmann, Joanna Jaworska, Thomas B. Knudsen, Robert Landsiedel, Marcel Leist, Paul Locke, Gavin Maxwell, James Mckim, Emily Mcvey, Gladys Ouédraogo, Grace Patlewicz, Olavi Pelkonen, Erwin Roggen, Costanza Rovida, Irmela Ruhdel, Michael Schwarz, Andreas Schepky, Greet Schoeters, Nigel Skinner, Kerstin Trentz, Marian Turner, Philippe Vanparys, James Yager, Joanne Zurlo, Thomas Hartung

Biomedical Research and Alternative Methods Collection

Systemic toxicity testing forms the cornerstone for the safety evaluation of substances. Pressures to move from traditional animal models to novel technologies arise from various concerns, including: the need to evaluate large numbers of previously untested chemicals and new products (such as nanoparticles or cell therapies), the limited predictivity of traditional tests for human health effects, duration and costs of current approaches, and animal welfare considerations. The latter holds especially true in the context of the scheduled 2013 marketing ban on cosmetic ingredients tested for systemic toxicity. Based on a major analysis of the status of alternative methods (Adler et …

Animal Learning And Training: Implications For Animal Welfare, Sabrina I.C.A. Brando

Assessment of Animal Welfare Collection

KEY POINTS

• A definition of animal welfare is the state of the individual as it attempts to cope with its environment.
• Welfare concerns all of the mechanisms for coping, involving physiology, behavior, feelings, and pathologic responses.
• When training programs are not in place the animal’s welfare could be impaired.
• Some of the behaviors an animal exhibits can be used to gain insight into how the animal feels about the environment, caretakers, and procedures.
• Many contemporary animal trainers and care specialists focus on building relationships using positive reinforcement.

Extended Grazing: A Detailed Analysis Of Irish Dairy Farms, D. Läpple, T. Hennessy, M. O'Donovan

Farm Animal Husbandry Collection

Profitability and factors affecting grazing season length were econometrically analyzed using a representative sample of Irish dairy farms. The objective of this study was to explore what potential exists on Irish dairy farms to extend the grazing season and to quantify the possible economic benefits that result from lengthening the grazing season. Regression results indicate that location factors affect the length of the grazing season, but even when physical factors are controlled, farmer characteristics, such as education, also affect the grazing season length. The results of a panel data analysis show that significant cost reductions can be achieved by extending …

Mammary Cell Activity And Turnover In Dairy Cows Treated With The Prolactin-Release Inhibitor Quinagolide And Milked Once Daily, M. Boutinaud, V. Lollivier, L. Finot, R. M. Bruckmaier, P. Lacasse

Physiology Collection

To assess the regulation of mammary cell activity, survival, and proliferation by prolactin (PRL), 5 Holstein cows in early lactation received daily i.m. injections of 1 mg of quinagolide, a suppressor of PRL release, for 9 wk, whereas 4 control cows received the vehicle (water) only. During the last week of treatment, one udder half was milked once a day (1×) and the other twice a day (2×). Mammary biopsies were harvested 1 wk before and 4 and 8 wk after the start of quinagolide treatment. The quinagolide injections reduced milk yield and resulted in lower levels of κ-casein and …

Outdoor Cats: An Animal Welfare And Protection Perspective, John Hadidian, Inga Gibson, Susan Hagood, Nancy Peterson, Bernard Unti, Betsy Mcfarland, Katie Lisnik, Heather Bialy, Inga Fricke, Kathleen Schatzmann, Jennifer Fearing, Pam Runquist, Andrew N. Rowan (Ed.)

Stray and Feral Animal Populations Collection

First raised as a serious conservation issue more than 100 years ago, the impact of free-roaming cats on wildlife has been a subject of debate, controversy, and conflict since then. Cats have been tied directly to the extinction of sensitive species in island environments and implicated as major threats to certain wildlife populations elsewhere. Yet the study of free-roaming cats and the problems attributed to them lags behind the standards of research typical with more traditional vertebrate “pest” species. Alternative management approaches, ranging from traditional practices such as removal and depopulation to emerging concepts such as Trap- Neuter-Return (TNR), have …

A Non-Invasive Assay For Monitoring Stress Responses: A Comparison Between Wild And Captive-Reared Rainbowfish (Melanoteania Duboulayi), Amina Zuberi, Sinan Ali, Culum Brown

Aquaculture Collection

The stress response of wild and captive reared rainbowfish (Melanoteania duboulayi) following chasing by a simulated predator was examined. Cortisol release rate was monitored using a flow through system by measuring water borne hormone levels. Tests using known cortisol concentrations revealed that the technique yielded 95% of the cortisol present in the water. Cortisol release rates increased several fold in both populations after being chased but peaked at different time periods. Wild fish showed a typical stress response with release rate rising to (2.29±0.22 ng g⁻¹ h⁻¹) 2 h after exposure followed by rapid recovery. The captive-reared …

Animal Pain: What It Is And Why It Matters, Bernard E. Rollin

Animal Welfare Collection

The basis of having a direct moral obligation to an entity is that what we do to that entity matters to it. The ability to experience pain is a sufficient condition for a being to be morally considerable. But the ability to feel pain is not a necessary condition for moral considerability. Organisms could have possibly evolved so as to be motivated to flee danger or injury or to eat or drink not by pain, but by ‘‘pangs of pleasure’’ that increase as one fills the relevant need or escapes the harm. In such a world, ‘‘mattering’’ would be positive, …

Lessons From Chimpanzee-Based Research On Human Disease: The Implications Of Genetic Differences, Jarrod Bailey

Laboratory Experiments Collection

Assertions that the use of chimpanzees to investigate human diseases is valid scientifically are frequently based on a reported 98–99% genetic similarity between the species. Critical analyses of the relevance of chimpanzee studies to human biology, however, indicate that this genetic similarity does not result in sufficient physiological similarity for the chimpanzee to constitute a good model for research, and furthermore, that chimpanzee data do not translate well to progress in clinical practice for humans. Leading examples include the minimal citations of chimpanzee research that is relevant to human medicine, the highly different pathology of HIV/AIDS and hepatitis C virus …

Learned Recognition And Avoidance Of Invasive Mosquitofish By The Shrimp, Paratya Australiensis, Joshua D. Bool, Kristen Whitcomb, Erin Kydd, Culum Brown

Sentience Collection

Little is known about the learning ability of crustaceans, especially with respect to their anti-predator responses to invasive species. In many vertebrates, anti-predator behaviour is influenced by experience during ontogeny. Here, predator-naïve glass shrimp (Paratya australiensisis) were exposed to a predatory, invasive fish species, Gambusia holbrooki, to determine whether shrimp could learn to: (1) avoid the scent of Gambusia via classical conditioning; and (2) restrict their activity patterns to the night to reduce predatory encounters. Conditioned shrimp were placed in containers in aquaria containing Gambusia for 3 days during which time they could be harassed but not consumed by Gambusia. …

Harnessing Opportunities In Non-Animal Asthma Research For A 21st-Century Science, Gemma L. Buckland

Experimentation Collection

The incidence of asthma is on the increase and calls for research are growing, yet asthma is a disease that scientists are still trying to come to grips with. Asthma research has relied heavily on animal use; however, in light of increasingly robust in vitro and computational models and the need to more fully incorporate the ‘Three Rs’ principles of Replacement, Reduction and Refinement, is it time to reassess the asthma research paradigm? Progress in non-animal research techniques is reaching a level where commitment and integration are necessary. Many scientists believe that progress in this field rests on linking disciplines …

The Evolution Of Lateralized Foot Use In Parrots: A Phylogenetic Approach, Culum Brown, Maria Magat

Sentience Collection

Cerebral lateralization refers to the division of cognitive function in either brain hemisphere and may be overtly expressed as behavioral asymmetries, such as handedness. The evolutionary history of laterality is of considerable interest due to its close link with the development of human language. Although considerable research effort has aimed at the proximate explanations of cerebral lateralization, considerably less attention has been paid to ultimate explanations. The extent to which laterality is constrained by phylogeny or shaped by ecological forces through natural selection has received little attention. Here, the foot preference of 23 species of Australian parrots was examined to …