Life Sciences Commons^™

Applying Ethological And Health Indicators To Practical Animal Welfare Assessment, F. Wemelsfelder, S. Mullan

Animal Welfare Collection

There is a growing effort worldwide to develop objective indicators for animal welfare assessment, which provide information on an animal’s quality of life, are scientifically trustworthy, and can readily be used in practice by professionals. Animals are sentient beings capable of positive and negative emotion, and so these indicators should be sensitive not only to their physical health, but also to their experience of the conditions in which they live. This paper provides an outline of ethological research aimed at developing practical welfare assessment protocols. The first section focuses on the development and validation of welfare indicators generally, in terms …

Qualitative Behavioural Assessment Of The Motivation For Feed In Sheep In Response To Altered Body Condition Score, C. A. Stockman, T. Collins, A. L. Barnes, D. Miller, S. L. Wickham, E. Verbeek, L. Matthews, D. Ferguson, F. Wemelsfelder, P. A. Fleming

Sentience Collection

Qualitative behavioural assessment (QBA) has been used to quantify the expressive behaviour of animals, and operant tests have been used to quantify measures of behavioural need. In this study we compared measures of behavioural expression and behaviour in operant tests. We examined the behavioural expression of pregnant ewes of body condition score (BCS) 2 and 3. The ewes were exposed to a feed motivation test in which they received a food reward. Pregnant ewes (48–70 days gestation) were assessed during a food motivation test after they had been maintained at BCS 3 (n = 7) or given a decreasing plane …

The Usefulness Of Systematic Reviews Of Animal Experiments For The Design Of Preclinical And Clinical Studies, Rob B.M. De Vries, Kimberley E. Weaver, Marc T. Avey, Martin Stephens, Emily S. Sena, Marlies Leenaars

Experimentation Collection

The question of how animal studies should be designed, conducted, and analyzed remains underexposed in societal debates on animal experimentation. This is not only a scientific but also amoral question. After all, if animal experiments are not appropriately designed, conducted, and analyzed, the results produced are unlikely to be reliable and the animals have in effect been wasted. In this article, we focus on one particular method to address this moral question, namely systematic reviews of previously performed animal experiments. We discuss how the design, conduct, and analysis of future (animal and human) experiments may be optimized through such systematic …

Consensus Report On The Future Of Animal-Free Systemic Toxicity Testing, Marcel Leist, Nina Hasiwa, Costanza Rovida, Mardas Daneshian, David Basketter, Ian Kimber, Harvey Clewell, Tilman Gocht, Alan Goldberg, Francois Busquet, Anna-Maria Rossi, Michael Schwarz, Martin Stephens, Rob Taalman, Thomas B. Knudsen, James Mckim, Georgina Harris, David Pamies, Thomas Hartung

Experimentation Collection

Since March 2013, animal use for cosmetics testing for the European market has been banned. This requires a renewed view on risk assessment in this field. However, in other fields as well, traditional animal experimentation does not always satisfy requirements in safety testing, as the need for human-relevant information is ever increasing. A general strategy for animal-free test approaches was outlined by the US National Research Council’s vision document for Toxicity Testing in the 21st Century in 2007. It is now possible to provide a more defined roadmap on how to implement this vision for the four principal areas of …

Acoustic Divergence In The Rut Vocalizations Of Persian And European Fallow Deer, J. B. Stachowicz, E. Vannoni, B. J. Pitcher, Elodie F. Briefer, E. Geffen, A. G. Mcelligott

Veterinary Science and Medicine Collection

We conducted a study of the male rut vocalizations (groans) of two closely related species, Persian and European fallow deer. Persian fallow deer are endangered, restricted to Iran and Israel, and their rut vocalizations have never been studied. By contrast, European fallow deer are one of the most common deer species in the world, and have been the subject of numerous detailed studies. Persian bucks are approximately 16% larger than European bucks, and this can have important implications for vocalizations. Persian bucks were recorded in Israel, and European bucks were recorded in the UK and Ireland. We measured temporal, fundamental …

Apes Communicate About Absent And Displaced Objects: Methodology Matters, Heidi Lyn, Jamie L. Russell, David A. Leavens, Kim A. Bard, Sarah T. Boysen, Jennifer A. Schaeffer, William D. Hopkins

Sentience Collection

Displaced reference is the ability to refer to an item that has been moved (displaced) in space and/ or time, and has been called one of the true hallmarks of referential communication. Several studies suggest that nonhuman primates have this capability, but a recent experiment concluded that in a specific situation (absent entities) human infants display displaced reference but chimpanzees do not. Here we show that chimpanzees and bonobos of diverse rearing histories are capable of displaced reference to absent and displaced objects. It is likely that some of the conflicting findings from animal cognition studies are due to relatively …

Effect Of Confinement On Water-Borne And Whole Body Cortisol In Wild And Captive-Eared Rainbowfish (Melanoteania Duboulayi), Amina Zuberi, Culum Brown, Sinan Ali

Physiology Collection

Whole body and water-borne cortisol levels were measured in captive reared and wild Rainbowfish (Melanoteania duboulayi Castelnau 1878) subjected to social isolation by confining them in a beaker for 30 min to induce an acute stress response. Wild fish had higher levels of cortisol before and after exposure to a mild stressor and also showed the greatest stress response. The differences in stress responses are likely the result of artificial selection in the captive environment. Importantly, there was a strong linear relationship between whole body and water-borne cortisol in wild and captive reared populations (r² =0. 95 and 0.84, …

Population Genetic Structure Of Gray Wolves (Canis Lupus) In A Marine Archipelago Suggests Island-Mainland Differentiation Consistent With Dietary Niche, Astrid V. Stronen, Erin L. Navid, Michael S. Quinn, Paul C. Paquet, Heather M. Bryan, Chris T. Darimont

Population Distribution and Habitat Collection

Background: Emerging evidence suggests that ecological heterogeneity across space can influence the genetic structure of populations, including that of long-distance dispersers such as large carnivores. On the central coast of British Columbia, Canada, wolf (Canis lupus L., 1758) dietary niche and parasite prevalence data indicate strong ecological divergence between marine-oriented wolves inhabiting islands and individuals on the coastal mainland that interact primarily with terrestrial prey. Local holders of traditional ecological knowledge, who distinguish between mainland and island wolf forms, also informed our hypothesis that genetic differentiation might occur between wolves from these adjacent environments.

Results: We used microsatellite genetic markers …

Grizzly Bear Monitoring By The Heiltsuk People As A Crucible For First Nation Conservation Practice, William G. Housty, Anna Noson, Gerald W. Scoville, John Boulanger, Richard M. Jeo, Chris T. Darimont, Christopher E. Filardi

Wildlife Population Management Collection

Guided by deeply held cultural values, First Nations in Canada are rapidly regaining legal authority to manage natural resources. We present a research collaboration among academics, tribal government, provincial and federal government, resource managers, conservation practitioners, and community leaders supporting First Nation resource authority and stewardship. First, we present results from a molecular genetics study of grizzly bears inhabiting an important conservation area within the territory of the Heiltsuk First Nation in coastal British Columbia. Noninvasive hair sampling occurred between 2006 and 2009 in the Koeye watershed, a stronghold for grizzly bears, salmon, and Heiltsuk people. Molecular demographic analyses revealed …

Stress And Reproductive Hormones Reflect Inter-Specific Social And Nutritional Conditions Mediated By Resource Availability In A Bear–Salmon System, Heather M. Bryan, Chris T. Darimont, Paul C. Paquet, Katherine E. Wynne-Edwards, Judit E. G. Smits

Physiology Collection

Food availability can influence the nutritional and social dynamics within and among species. Our investigation focused on grizzly and black bears in coastal British Columbia, Canada, where recent and dramatic declines in their primary prey (salmon) raise concerns about potentially negative effects on bear physiology. We examined how salmon availability relates to stress and reproductive hormones in coastal grizzly (n = 69) and black bears (n = 68) using cortisol and testosterone. In hair samples from genotyped individuals, we quantified salmon consumption using stable isotope analysis and hormone levels by enzyme immunoassay. To estimate the salmon biomass available to each …

Lost In Translation: Animal Models And Clinical Trials In Cancer Treatment, Isabella W.Y. Mak, Nathan Evaniew, Michelle Ghert

Human Clinical Trials Collection

Due to practical and ethical concerns associated with human experimentation, animal models have been essential in cancer research. However, the average rate of successful translation from animal models to clinical cancer trials is less than 8%. Animal models are limited in their ability to mimic the extremely complex process of human carcinogenesis, physiology and progression. Therefore the safety and efficacy identified in animal studies is generally not translated to human trials. Animal models can serve as an important source of in vivo information, but alternative translational approaches have emerged that may eventually replace the link between in vitro studies and …

Spatial Analysis Of Factors Influencing Long-Term Stress In The Grizzly Bear (Ursus Arctos) Population Of Alberta, Canada, Mathieu Bourbonnais, Trisalyn Nelson, Mark Cattet, Chris T. Darimont, Gordon Stenhouse

Physiology Collection

Non-invasive measures for assessing long-term stress in free ranging mammals are an increasingly important approach for understanding physiological responses to landscape conditions. Using a spatially and temporally expansive dataset of hair cortisol concentrations (HCC) generated from a threatened grizzly bear (Ursus arctos) population in Alberta, Canada, we quantified how variables representing habitat conditions and anthropogenic disturbance impact long-term stress in grizzly bears. We characterized spatial variability in male and female HCC point data using kernel density estimation and quantified variable influence on spatial patterns of male and female HCC stress surfaces using random forests. Separate models were developed for regions …

Dog Behavior Co-Varies With Height, Bodyweight And Skull Shape, Paul D. Mcgreevy, Dana Georgevsky, Johanna Carrasco, Michael Valenzuela, Deborah L. Duffy, James A. Serpell

Eidonomy Collection

Dogs offer unique opportunities to study correlations between morphology and behavior because skull shapes and body shape are so diverse among breeds. Several studies have shown relationships between canine cephalic index (CI: the ratio of skull width to skull length) and neural architecture. Data on the CI of adult, show-quality dogs (six males and six females) were sourced in Australia along with existing data on the breeds’ height, bodyweight and related to data on 36 behavioral traits of companion dogs (n = 8,301) of various common breeds (n = 49) collected internationally using the Canine Behavioral Assessment and Research Questionnaire …

Observing The Unwatchable Through Acceleration Logging Of Animal Behavior, Danielle D. Brown, Roland Kays, Martin Wikelski, Rory Wilson, A. Peter Klimley

Methodology and Animal Models in Research

Behavior is an important mechanism of evolution and it is paid for through energy expenditure. Nevertheless, field biologists can rarely observe animals for more than a fraction of their daily activities and attempts to quantify behavior for modeling ecological processes often exclude cryptic yet important behavioral events. Over the past few years, an explosion of research on remote monitoring of animal behavior using acceleration sensors has smashed the decades-old limits of observational studies. Animal-attached accelerometers measure the change in velocity of the body over time and can quantify fine-scale movements and body postures unlimited by visibility, observer bias, or the …

Photo-Identification Methods Reveal Seasonal And Long-Term Site-Fidelity Of Risso’S Dolphins (Grampus Griseus) In Shallow Waters (Cardigan Bay, Wales), Marijke N. De Boer, Josephine Clark, Mardik F. Leopold, Mark P. Simmonds, Peter J.H. Reijnders

Ecology Collection

A photo-identification study on Risso’s dolphins was carried out off Bardsey Island in Wales (July to September, 1997-2007). Their local abundance was estimated using two different analytical techniques: 1) mark-recapture of well-marked dolphins using a “closed-population” model; and 2) a census technique based on the total number of iden-tified individual dolphins sighted over the study period. The mark-recapture estimates of 121 (left sides; 64 - 178, 95% CI; CV 0.24) and 145 dolphins (right sides; 78 - 213, 95% CI; CV 0.24) closely matched the census technique estimates (population size of 90 - 151). It was found that the dolphins …

Longevity And Mortality Of Owned Dogs In England, D. G. O'Neill, D. B. Church, P. D. Mcgreevy, P. C. Thomson, D. C. Brodbelt

Epidemiology Collection

Improved understanding of longevity represents a significant welfare opportunity for the domestic dog, given its unparalleled morphological diversity. Epidemiological research using electronic patient records (EPRs) collected from primary veterinary practices overcomes many inherent limitations of referral clinic, owner questionnaire and pet insurance data. Clinical health data from 102,609 owned dogs attending first opinion veterinary practices (n = 86) in central and southeast England were analysed, focusing on 5095 confirmed deaths.

Of deceased dogs with information available, 3961 (77.9%) were purebred, 2386 (47.0%) were female, 2528 (49.8%) were neutered and 1105 (21.7%) were insured. The overall median longevity was 12.0 years …

Stress And Reproductive Hormones In Grizzly Bears Reflect Nutritional Benefits And Social Consequences Of A Salmon Foraging Niche, Heather M. Bryan, Chris T. Darimont, Paul C. Paquet, Katherine E. Wynne-Edwards, Judit E. G. Smits

Physiology Collection

Physiological indicators of social and nutritional stress can provide insight into the responses of species to changes in food availability. In coastal British Columbia, Canada, grizzly bears evolved with spawning salmon as an abundant but spatially and temporally constrained food source. Recent and dramatic declines in salmon might have negative consequences on bear health and ultimately fitness. To examine broadly the chronic endocrine effects of a salmon niche, we compared cortisol, progesterone, and testosterone levels in hair from salmon-eating bears from coastal BC (n = 75) with the levels in a reference population from interior BC lacking access to salmon …

Laterality Influences Schooling Position In Rainbowfish, Melanotaenia Spp, Anne-Laurence Bibost, Culum Brown

Aquaculture Collection

Cerebral lateralization is a widespread trait among animals, is often manifested as side biases in behaviour (laterality) and has been suggested to provide fitness benefits. Here we examined the influence of laterality on the organisation of fish schools using rainbowfish (Melanotaenia spp) as model species. The pattern and strength of laterality for each individual was determined by examining eye preferences whilst examining their reflection in a mirror. Schools of four fish of known laterality were then created and the preferred position for each fish within the school was repeatedly observed in a flume. Fish which showed right eye preferences in …

Confronting Uncertainty In Wildlife Management: Performance Of Grizzly Bear Management, Kyle A. Artelle, Sean C. Anderson, Andrew B. Cooper, Paul C. Paquet, John D. Reynolds, Chris T. Darimont

Wildlife Population Management Collection

Scientific management of wildlife requires confronting the complexities of natural and social systems. Uncertainty poses a central problem. Whereas the importance of considering uncertainty has been widely discussed, studies of the effects of unaddressed uncertainty on real management systems have been rare. We examined the effects of outcome uncertainty and components of biological uncertainty on hunt management performance, illustrated with grizzly bears (Ursus arctos horribilis) in British Columbia, Canada. We found that both forms of uncertainty can have serious impacts on management performance. Outcome uncertainty alone – discrepancy between expected and realized mortality levels – led to excess mortality in …

An Analysis Of The Use Of Dogs In Predicting Human Toxicology And Drug Safety, Jarrod Bailey, Michelle Thew, Michael Balls

Laboratory Experiments Collection

Dogs remain the main non-rodent species in preclinical drug development. Despite the current dearth of new drug approvals and meagre pipelines, this continues, with little supportive evidence of its value or necessity. To estimate the evidential weight provided by canine data to the probability that a new drug may be toxic to humans, we have calculated Likelihood Ratios (LRs) for an extensive dataset of 2,366 drugs with both animal and human data, including tissue-level effects and Medical Dictionary for Regulatory Activities (MedDRA) Level 1–4 biomedical observations. The resulting LRs show that the absence of toxicity in dogs provides virtually no …

Incorporating New Technologies Into Toxicity Testing And Risk Assessment: Moving From 21st Century Vision To A Data-Driven Framework, Russell S. Thomas, Martin A. Philbert, Scott Auerbach, Barbara A. Wetmore, Michael J. Devito, Ila Cote, J. Craig Rowlands, Maurice Whelan, Sean M. Hays, Melvin E. Andersen, M.E. (Bette) Meek, Lawrence W. Reiter, Jason C. Lambert, Harvey J. Clewell Iii, Martin L. Stephens, Q. Jay Zhao, Scott C. Wesselkamper, Lynn Flowers, Edward W. Carney, Tim P. Pastoor, Dan D. Petersen, Carole L. Yauk, Andy Nong

in Vitro Research Models Collection

Based on existing data and previous work, a series of studies is proposed as a basis toward a pragmatic early step in transforming toxicity testing. These studies were assembled into a data-driven framework that invokes successive tiers of testing with margin of exposure (MOE) as the primary metric. The first tier of the framework integrates data from high-throughput in vitro assays, in vitro-to-in vivo extrapolation (IVIVE) pharmacokinetic modeling, and exposure modeling. The in vitro assays are used to separate chemicals based on their relative selectivity in interacting with biological targets and identify the concentration at which these interactions …

Discrimination Of Familiar Human Faces In Dogs (Canis Familiaris), Ludwig Huber, Anaïs Racca, Billy Scaf, Zsófia Virányi, Friederike Range

Recognition Collection

Faces are an important visual category for many taxa, and the human face is no exception to this. Because faces differ in subtle ways and possess many idiosyncratic features, they provide a rich source of perceptual cues. A fair amount of those cues are learned through social interactions and are used for future identification of individual humans. These effects of individual experience can be studied particularly well in hetero-specific face perception. Domestic dogs represent a perfect model in this respect, due to their proved ability to extract important information from the human face in socio-communicative interactions. There is also …

Predator Recognition In Rainbowfish, Melanotaenia Duboulayi, Embryos, Lois J. Oulton, Vivian Haviland, Culum Brown

Aquaculture Collection

Exposure to olfactory cues during embryonic development can have long term impacts on birds and amphibians behaviour. Despite the vast literature on predator recognition and responses in fishes, few researchers have determined how fish embryos respond to predator cues. Here we exposed four-day-old rainbowfish (Melanotaenia duboulayi) embryos to cues emanating from a novel predator, a native predator and injured conspecifics. Their response was assessed by monitoring heart rate and hatch time. Results showed that embryos have an innate capacity to differentiate between cues as illustrated by faster heart rates relative to controls. The greatest increase in heart rate occurred in …

A Framework To Evaluate Wildlife Feeding In Research, Wildlife Management, Tourism And Recreation, Sara Dubois, David Fraser

Wildlife Population Management Collection

Feeding of wildlife occurs in the context of research, wildlife management, tourism and in opportunistic ways. A review of examples shows that although feeding is often motivated by good intentions, it can lead to problems of public safety and conservation and be detrimental to the welfare of the animals. Examples from British Columbia illustrate the problems (nuisance animal activity, public safety risk) and consequences (culling, translocation) that often arise from uncontrolled feeding. Three features of wildlife feeding can be distinguished: the feasibility of control, the effects on conservation and the effects on animal welfare. An evaluative framework incorporating these three …

Goats Favour Personal Over Social Information In An Experimental Foraging Task, Luigi Baciadonna, Alan G. Mcelligott, Elodie F. Briefer

Ethology Collection

Animals can use their environments more efficiently by selecting particular sources of information (personal or social), according to specific situations. Group-living animals may benefit from gaining information based on the behaviour of other individuals. Indeed, social information is assumed to be faster and less costly to use than personal information, thus increasing foraging efficiency. However, when food sources change seasonally or are randomly distributed, individual information may become more reliable than social information. The aim of this study was to test the use of conflicting personal versus social information in goats (Capra hircus), in a foraging task.We found that goats …

Local Attitudes Towards Bear Management After Illegal Feeding And Problem Bear Activity, Sara Dubois, David Fraser

Wildlife Population Management Collection

The “pot bears” received international media attention in 2010 after police discovered the intentional feeding of over 20 black bears during the investigation of an alleged marijuana-growing operation in Christina Lake, British Columbia, Canada. A two-phase random digit dialing survey of the community was conducted in 2011 to understand local perspectives on bear policy and management, before and after a summer of problem bear activity and government interventions. Of the 159 households surveyed in February 2011, most had neutral or positive attitudes towards bears in general, and supported the initial decision to feed the food-conditioned bears until the autumn hibernation. …

A Note On Pigs’ Knowledge Of Hidden Objects, Christian Nawroth, Mirjam Ebersbach, Eberhard Von Borell

Memory Collection

Object permanence is the notion that objects continue to exist even when they are out of observer’s sight. This ability is adaptive for free ranging animals who have to cope with a dangerous and highly changeable environment and allows them to be aware of predators sneaking in their proximity or to keep track of conspecifics or food sources, even when out of sight. Farm animals might also benefit from object permanence because the ability to follow the trajectory of hidden food or objects may lead to a higher predictability of subjects’ environment, which in turn might affect the level of …

Examining The Link Between Personality And Laterality In A Feral Guppy Poecilia Reticulata Population, Eleanor Irving, Culum Brown

Sentience Collection

This study examined whether variation in the strength and direction of lateralization in a detour task was linked with variation in three common personality measurements: boldness, activity and sociability, in a population of wild guppies Poecilia reticulata. Additionally, the aim was to determine whether any consistent correlations between these behavioural traits, known as behavioural syndromes, were present in the study population. The results revealed that all three personality traits were highly repeatable over time in both sexes. Evidence of a complex syndrome in the form of a correlation between boldness, sociability and activity was found; however, this relationship was only …

Real-Time Bioacoustics Monitoring And Automated Species Identification, T. Mitchell Aide, Carlos Corrada-Bravo, Marconi Campos-Cerqueira, Carlos Milan, Giovany Vega, Rafael Alvarez

Bioacoustics Collection

Traditionally, animal species diversity and abundance is assessed using a variety of methods that are generally costly, limited in space and time, and most importantly, they rarely include a permanent record. Given the urgency of climate change and the loss of habitat, it is vital that we use new technologies to improve and expand global biodiversity monitoring to thousands of sites around the world. In this article, we describe the acoustical component of the Automated Remote Biodiversity Monitoring Network (ARBIMON), a novel combination of hardware and software for automating data acquisition, data management, and species identification based on audio recordings. …

An Hsus Report: Industrial Farm Animal Production And Livestock Associated Mrsa (Methicillin Resistant Staphylococcus Aureus), The Humane Society Of The United States

Impact of Animal Agriculture

Staphylococcus aureus is a leading cause of bacterial infection and is increasingly found to be resistant to antibiotic therapy. A newly described type of Methicillin Resistant Staphylococcus aureus carried by farm animals, Livestock Associated MRSA (LA-MRSA), is now causing infections in humans with and without direct livestock contact. A reduction in the non-therapeutic use of antibiotics in feed would likely reduce the capacity of industrial animal agriculture to continue to create, disseminate, and perpetuate a large reservoir of LA-MRSA on a global scale, but more fundamental changes in the way animals are raised for food may be necessary forestall a …