Life Sciences Commons^™

Investigating Antimicrobial Activity Of An Unknown Isolate Against Eskape Pathogens, Kaden Youssef, Courtney Williams, Parker Woods

Student Research Symposium

ESKAPE pathogens (e.g., S. aureus, P. aeruginosa) are responsible for many antibiotic-resistant infections. There is a growing need to discover new antimicrobial agents. This experiment investigates whether an unknown bacterial isolate exhibits antimicrobial activity. Our isolate previously showed antimicrobial activity against Staphylococcus epidermidis, suggesting it may have similar effects on Staphylococcus aureus.

Alternatives For Whey Protein Powder, Alleyah Murphy, Emily Jensen, Jaiden C., Spencer C.

Student Research Symposium

Protein shakes have become a popular product in the market, heavily advertised as convenient nutrient sources for active adolescents and adults. These products are widely available in grocery stores, pharmacies, and through online distributors (Deutch, 2022). Typically made with whey protein, they are unsuitable for individuals following vegan or lactose-free diets. The goal of this experiment is to evaluate the impact of substituting whey protein powder with equivalent amounts of pea, soy, and hemp seed protein powders on the taste, texture, viscosity, and protein content of vanilla protein shakes to identify the most suitable alternative

Plant-Based Substitutes For Gelatin In Gummy Bears, Neisha Christensen, Tiffany Stoker, Bethany Janes, Kaiah Thomas, Maryann Schneider

Student Research Symposium

Gummy bears are typically made using gelatin. Those who choose to follow a vegan/vegetarian diet are unable to eat products that contain gelatin, since gelatin is made from the collagen found in animal tissues. Additionally, people who avoid eating pork for religious or other reasons cannot consume gelatin, as it is commonly derived from pork.

Reliability Of Novel Morphological Features Of The Auditory Brainstem Response, Marlana Petersen

Student Research Symposium

Pure-tone audiometry can detect hearing loss but does not determine specific site-of-lesion. It is not sensitive to many disorders, especially neural disorders that impact hearing

Seed Dormancy In Blue Elderberry (Sambucus Cerulea), Stephanie Spencer

Student Research Symposium

• Health benefits: cold remedy, antioxidants, anti- inflammatory (Ulbricht et al., 2014)

• Food uses: wines, syrups, jams, drinks, and vitamin supplements (Cai et al., 2024)

• Increasing interest: from 6.9% to 41.2% of survey respondents between 2011 and 2022 (Cai et al., 2024)

Aging In Motion: The Impact Of Activity On Visceral Fat In Senior Individuals, Heather Feeny, Paxton Castillon, Alyssa Mcnutt, Mattew Densley

Student Research Symposium

The aim of this study is to investigate activity levels, including sleep duration, sitting time, and their relationship with visceral fat area (VFA).

Induction Of Diabetic Cardiomyopathy In A Three-Dimensional Myocardial Model, Maelyn Andreasen

Student Research Symposium

Diabetic cardiomyopathy: Impairs and damages heart tissue through chronic exposure to hyperglycemia, or high blood sugar, in diabetic individuals

Body Composition Of Older Active Adults: The Huntsman Senior Games Study, Abby Hansen, Paxton Castillon, Heather Feeny, Alyssa Mcnutt, Matthew Densley, Elizabeth Cafferty

Student Research Symposium

• Body Fat Percentage (%BF): ratio of adipose tissue to total mass

The Effects Of Biodiversity On Plant Parasitic Nematodes, Josh Lund

Student Research Symposium

The Dilution Effect Hypothesis¹ argues that biodiversity reduces pathogen transmission risk due to dilution of susceptible hosts, enhanced microbial communities that suppress pathogens, interference competition, nutrient heterogeneity, and ecological stability.

The Effects Of Different Types Of Fertilizer On The Growth Of Pseudomonas Frederiksbergenis, Aspen Curtis, Nadia Greding, Kylie Fluckiger, Samantha Clements

Student Research Symposium

Which fertilizers will promote the growth of the isolate of Pseudomonas frederiksbergensis (P. frederiksbergensis)?

Pseudomonas Parafulva Confirmed To Have Antifungal Properties When Grown In Conjunction With Botrytis Cinerea, Caden Dupee, Kylee Eldredge, Zachary Elmer, Joshua Hartvigsen

Student Research Symposium

Widespread antibiotic use has led to antibiotic resistance of infectious bacteria. (Young, 2017) As part of the TinyEarth Program, the lab group collected and tested local soil samples for bacteria with hopes of finding novel antibiotic properties. From the soil samples, a bacteria was isolated, subsequently sequenced, and determined to be Pseudomonas parafulva. P. parafulva is a Gram-negative bacterium that has been known to frequent rice paddies of eastern Asian countries and has been collected in select regions of North America, including that of Cache Valley. P. parafulva has been linked to anti-fungal activities on plants. (Lui et …

Diets And Environmental Impacts - Generational Outlook, Aspen Hopper, Melanie Jex

Student Research Symposium

How different diets impact the environment: Are we aware?

Determining The Kinetic Basis Of Crispr-Cas12a2 Function, Sobita Kunwar, Thomson Hallmark, Dylan Keiser, Bronson Naegle, Sudeshna Manna, Aaron Thomas, Chase L. Beisel

Student Research Symposium

CRISPR- Cas (Clustered Regularly Interspaced Short Palindromic Repeats- CRISPR associated) systems are bacterial adaptive immune systems that protect bacteria against mobile genetic elements such as viruses and plasmids^1,2.

A Look Into Frugivorous Interactions With Psychotria And Palicourea In Barro Colorado Island, Panama, Madison Smart, Gerald Schneider, Elsa Jos

Student Research Symposium

Importance of Seed Dispersal

• Seed dispersal is a vital ecological process that influences plant population dynamics and ecosystem structure.
• Half of all seed-bearing plants rely on frugivorous animals to disperse their seeds to new locations, promoting plant recruitment

Investigating The Oxytocin System Of The Brain In The Molecular Basis Of Self-Control, Kenzie Wachtel

Student Research Symposium

• Oxytocin (OXT) is a neuropeptide involved in social behaviors such as pair-bonding, maternal behavior, and attachment¹. At the core of these social bonds is reward. OXT has many interactions with neural reward circuits to produce the pleasurable effects of social contact².

How Peribacillus Simplex Influences Plant Growth In The Presence Of Pesticide, David Tolman, Luke Wilkinson, Tanner Thompson, Tori Richardson

Student Research Symposium

P. simplex is known for its biocontrol, bioremediation, antibiotic producing, and sustainable agriculture properties

Protective Efficacy Of A Yellow Fever Virus Rna- Based Vaccine In A Hamster Model, Abby Sherman

Student Research Symposium

• Yellow Fever virus (YFV) remains a concern as of 2023, 34 countries in Africa and 13 countries in South America are endemic, with a case fatality rate of 11% shown in preliminary data.^[3]

Determining Antibiotic Efficacy On A Species Of Pseudomonas Bacteria Isolated From A Soil Sample, Austin Taylor, Peyton Wells, Alyssa Smith

Student Research Symposium

The Pseudomonas genus contains a variety of species of bacteria that play essential roles in soil health, plant growth, and microbial competition. Certain species exhibit biocontrol properties by producing antimicrobial compounds to protect plants from harmful pathogens. While our Pseudomonas species is normally non-pathogenic to plants and humans, it could transfer antimicrobial resistance genes to harmful pathogens in the environment. By studying the Minimum Inhibitory Concentration (MIC) of the isolated bacteria to several common antibiotics, we could identify antibiotic-sensitive strains that could be used in agriculture, or antimicrobial-producing strains that could serve as natural defenses for plants.

Identifying Compensatory Mutations In The Envelope Protein Of Japanese Encephalitis Virus That Restore Neurovirulence, Nathan Taylor, Jiyoun Kim

Student Research Symposium

• Japanese Encephalitis Virus (JEV) is a positive sense, single stranded Enveloped virus

Solving The Structure Of An Ancestral Protein Tyrosine Phosphatase, Natalie Turcsanski, Edna Armah

Student Research Symposium

Protein tyrosine phosphatases (PTPs) regulate cell signaling by removing phosphate groups from tyrosine residues. Malfunction of these enzymes can lead to diseases such as diabetes and cancer. A conserved feature among PTPs is the catalytic acid loop, essential for phosphate removal. Computational analysis of PTP structures revealed that ancestral PTPs have considerable variation in conformations of this loop, sparking interest in their further study. The Asgard group, identified as ancestral to eukaryotic PTPs, was successfully expressed and purified under optimized conditions. Current efforts focus on optimizing crystallization to obtain larger crystals for structural analysis. This project will investigate the allosteric …

Determining The Function Of Type Iv-B Crispr-Cas Systems, Eric Jenkins, Brendon Guinn

Student Research Symposium

CRISPR-Cas systems are a diverse group of prokaryotic adaptive immune systems that defend against Mobile Genetic Elements (MGEs) such as bacteriophages and plasmids. CRISPR-associated (Cas) proteins bind to guide RNAs (crRNAs) transcribed from a DNA repeat array (CRISPR array) containing spacers complementary to MGEs. Upon infection by a MGE, the Cas protein-crRNA complex binds to the target sequence in the invaders genome and cleaves the nucleic acid.

Distributor-Specific Variability In Balb/C Mouse Mortality Following Sars-Cov-2-Ma10 Infection, Taylor Kerr

Student Research Symposium

In recent years SARS-COV-2 has become a rapidly expanding area of research. To model human infections, this virus was adapted to mice enabling valuable insight into these processes.² Mice are often an effective research model due to their low cost, short reproduction time, and ability to adapt to a wide variety of viruses. Research into these immunological responses is crucial in developing vaccines, treatments, and furthering our understanding of this virus' behavior.¹ However, genetic variability in mouse models may be confounding previous and on-going research.

Activation Of Enkephalinergic Neurons Modulate Pain Within The Brain, Lucy N. Scribner, Oakley M. Statham, Greydon R. Durrant, Callie M. Newson

Student Research Symposium

The endogenous opioid system, consisting of three neuropeptides and canonical receptors, is ubiquitously expressed throughout the brain and is heavily involved in modulating pain[1]. However, research has placed tremendous focus on investigating the receptors of this system, and less attention has been devoted to the peptides. One such opioid peptide, enkephalin, is notably absent within the pain center of the brain, the periaqueductal gray (PAG)[2]. To further understand the role enkephalins play in modulating pain, we aimed to target and activate specific enkephalin-containing neurons that connect to the PAG. By activating these enkephalinergic projections, we hypothesized that this would lead …

Effects Of C-Section Birth On Vasopressin 1a Receptors In The Prairie Vole Brain, Caleb Hart

Student Research Symposium

The goal of this study was to understand how the brain might change depending on the birth experience: a vaginal birth or a cesarean section. C-sections have been associated with an increased risk of obesity and other behavioral and metabolic health conditions that are related to the function of hypothalamic hormones in the brain¹. Vasopressin is one neuropeptide hormone that is produced in the hypothalamus and influences a variety of metabolic functions and behaviors, including water balance and social pair bonding². This study used prairie voles to assess the effect of birth experience on the brain …

Mdpk Cell Lines Can Be Used For In Vitro Antiviral Testing With Highly Pathogenic Avian Influenza (H5n1), Jada L. Westover

Student Research Symposium

• Outbreak in agricultural poultry starting in 2022
• In March 2024 first confirmed case in Dairy Cattle
• Tested drugs T705, Oseltamivir, Baloxivir, Ribavirin, EIDD-2947, and EIDD-2749 on three different cell lines
• Goal: See if MDBK cell lines could be used for in vitro studies

Drivers Of Recruitment For A Population Of Cutthroat Trout In Northern Utah, Abbigail Lane, Austin Garner

Student Research Symposium

• Understanding recruitment dynamics informs effective management of sensitive populations (Camp et al. 2020).

Seasonal And Sex-Based Variation In Coyote Responses To Social Olfactory Stimuli, Giuliana Carneiro

Student Research Symposium

Coyotes (Canis latrans) are socially complex, often forming strong pair bonds that can persist across multiple breeding seasons³. Scent marking, particularly urine marking, is a key form of communication used to establish territory, convey reproductive status, and maintain social hierarchies¹. This behavior is influenced by factors such as sex and season, and is central to understanding their social dynamics⁴. This preliminary study is part of a broader investigation into coyote behavior, specifically examining how these factors affect responses to olfactory stimuli from familiar and unfamiliar individuals².

In this study, coyote …

Internship As An In-Vivo Technician At The Institute For Antiviral Research, Ashlynn Clark

Student Research Symposium

The Institute for Antiviral Research (IAR), located at Utah State University, specializes in evaluating antiviral agents and vaccines against a wide array of human pathogenic viruses, such as influenza, enterovirus, West Nile virus, yellow fever, zika virus, polio, and SARS-CoV-2, utilizing in vivo and in vitro models.

Response To Induced Herbivory, Isis Cordova

Student Research Symposium

Herbivore attacks trigger complex changes in plant primary and secondary metabolisms. Secondary metabolites (SM), are hypothesized to respond synergistically to repeated herbivore attacks (Fig. 1).

Creating A Stat2 Knock-Out Rat With Crispr/Cas9 Using I-Gonad, Isabella Dejesus, Yanan Liu, Don Joo, Nathan Merrill

Student Research Symposium

The STAT2 gene encodes a protein called Signal Transducer and Activator of Transcription 2. This protein is a part of the STAT family of transcription factors, which are involved in mediating cellular responses to various cytokines and growth factors, particularly those related to the immune response. Here, we established a successful procedure for creating a STAT2 knock-out (KO) Rat that can be used for more accurate disease testing of serious human ailments such as Lupus, Polio, Herpes, RSV, and even cancer.