Life Sciences Commons^™

Balancing Best Practice And Reality In Behavioral Intervention Development: A Survey Of Principal Investigators Funded By The National Institutes Of Health, Lauren Von Klinggraeff, Sarah Burkart, Christopher D. Pfledderer, Alexander Mclain, Bridget Armstrong Ph.D., Robert Glenn Weaver Med, Phd, Michael Beets Med, Mph, Phd

Faculty Publications

Preliminary studies play a prominent role in the development of large-scale behavioral interventions. Though recommendations exist to guide the execution and interpretation of preliminary studies, these assume optimal scenarios which may clash with realities faced by researchers. The purpose of this study was to explore how principal investigators (PIs) balance expectations when conducting preliminary studies. We surveyed PIs funded by the National Institutes of Health to conduct preliminary behavioral interventions between 2000 and 2020. Four hundred thirty-one PIs (19% response rate) completed the survey (November 2021 to January 2022, 72% female, mean 21 years post-terminal degree). Most PIs were aware …

Whole-Of-School Physical Activity Implementation In The Context Of The Dubai Fitness Challenge, Chris Mcmahon, Collin A. Webster, Robert Weaver, Christophe El Haber, Gönül Tekkurşun Demir, Zainab Mohamed Ismail, Syeda Zoha Fatima Naqvi, Mehnaz Ghani, Şevval Kepenek, Manel Kherraf, Thrisha Krishnakumar, Pranati Prakash, Yeowon Seo

Faculty Publications

Introduction

Physical activity (PA) promotion among school-aged youth is a global health priority. Recommendations for such promotion include implementing whole-of-school approaches that maximize resources across the school environment. This study examined schools’ participation in an annual, government-led, and emirate-wide initiative in Dubai, called the Dubai Fitness Challenge, in which the goal is to accrue 30 minutes of PA every day for 30 days (as such, the initiative is colloquially referred to as “Dubai 30x30”).

Methods

A mixed-methods design was employed for this study. Three schools were recruited using convenience sampling. Participants were 18 physical education teachers, 20 classroom teachers, 2 …

Unimer Suppression Enables Supersaturated Homopolymer Swollen Micelles With Long-Term Stability After Glassy Entrapment, Eric R. Williams, Christian X. Ruff, Morgan Stefik

Faculty Publications

Micelle sizes are critical for a range of applications where the simple ability to adjust and lock in specific stable sizes has remained largely elusive. While micelle swelling agents are well-known, their dynamic re-equilibration in solution implies limited stability. Here, a non-equilibrium processing sequence is studied where supersaturated homopolymer swelling is combined with glassy-core (“persistent”) micelles. This path-dependent process was found to sensitively depend on unimer concentration as revealed by DLS, SAXS, and TEM analysis. Here, lower-selectivity solvent combinations led to the formation of unimer-homopolymer aggregates and eventual precipitation, reminiscent of anomalous micellization. In contrast, higher-selectivity solvents enabled supersaturated homopolymer …

Unimer Suppression Enables Supersaturated Homopolymer Swollen Micelles With Long-Term Stability After Glassy Entrapment, Eric R. Williams, Christian X. Ruff, Morgan Stefik

Faculty Publications

Micelle sizes are critical for a range of applications where the simple ability to adjust and lock in specific stable sizes has remained largely elusive. While micelle swelling agents are well-known, their dynamic re-equilibration in solution implies limited stability. Here, a non-equilibrium processing sequence is studied where supersaturated homopolymer swelling is combined with glassy-core (“persistent”) micelles. This path-dependent process was found to sensitively depend on unimer concentration as revealed by DLS, SAXS, and TEM analysis. Here, lower-selectivity solvent combinations led to the formation of unimer-homopolymer aggregates and eventual precipitation, reminiscent of anomalous micellization. In contrast, higher-selectivity solvents enabled supersaturated homopolymer …

Left Ventricle Function And Post-Transcriptional Events With Exercise Training In Pigs, Stephanie L. Samani, Shayne C. Barlow, Lisa A. Freeburg, Traci L. Jones, Marlee Poole, Mark A. Sarzynski Ph.D., Faha, Facsm, Michael R. Zile, Tarek Shazly, Francis G. Spinale

Faculty Publications

Background

Standardized exercise protocols have been shown to improve overall cardiovascular fitness, but direct effects on left ventricular (LV) function, particularly diastolic function and relation to post-transcriptional molecular pathways (microRNAs (miRs)) are poorly understood. This project tested the central hypothesis that adaptive LV remodeling resulting from a large animal exercise training protocol, would be directly associated with specific miRs responsible for regulating pathways relevant to LV myocardial stiffness and geometry.

Methods and results

Pigs (n = 9; 25 Kg) underwent a 4 week exercise training protocol (10 degrees elevation, 2.5 mph, 10 min, 5 days/week) whereby LV chamber stiffness (KC) …

Ras/Mapk Signaling Mediates Adipose Tissue Control Of Ovarian Germline Survival And Ovulation In Drosophila Melanogaster, Tancia Bradshaw, Chad Simmons, Rachael Ott, Alissa Richmond Armstrong

Faculty Publications

From insects to humans, oogenesis is tightly linked to nutritional input, yet little is known about how whole organism physiology matches dietary changes with oocyte development. Considering that diet-induced adipose tissue dysfunction is associated with an increased risk for fertility problems, and other obesity-associated pathophysiologies, it is critical to decipher the cellular and molecular mechanisms linking adipose nutrient sensing to remote control of the ovary and other tissues. Our previous studies in Drosophila melanogaster have shown that amino acid sensing, via the amino acid response pathway and mTOR-mediated signaling function within adipocytes to control germline stem cell maintenance and ovulation, …

The Combined And Individual Effects Of Azadirachta Indica L. Oil And Nahco3 On The Inhibition Of F. Oxysporum, Neya Murugesan

Journal of the South Carolina Academy of Science

F. oxysporum is the causal agent of Fusarium wilt. This disease is known to penetrate the roots of plants and wilt the vascular system. The most common method for control is through the use of synthetic fungicides, which are known to cause adverse effects on the environment and human health. Thus, it is necessary that more methods of biocontrol be explored. Current literature shows that various salts and essential oils are effective for the biocontrol of F. oxysporum. However, the two types of substances have not yet been tested together, causing a gap in current research. Due to the antifungal …

The Effect Of Exposure To Lentinula Edodes And Flammulina Velutipes On The Biodegradation Of Crystallized Polylactic Acid, Iraine Taine

Journal of the South Carolina Academy of Science

Crystallized polylactic acid (CPLA) is a prevalent bioplastic which, despite being biodegradable, is extremely resistant to decomposition in natural environments. As a result, CPLA waste contamination is rapidly becoming a prevalent issue. Lentinula edodes and Flammulina velutipes are two widespread white-rot fungi whose capabilities for bioplastic degradation have not been examined. This study aimed to determine a natural approach for reducing plastic waste by evaluating the ability of L. edodes and F. velutipes to degrade CPLA bioplastic. It was hypothesized that CPLA samples would decrease in mass when individually treated with L. edodes and F. velutipes due to their similarities …

The Effect Of Various Polyphenols On Associative Memory Loss And Dementia In A Drosophila Melanogaster Model Of Alzheimer’S Disease, Anagha Gowda

Journal of the South Carolina Academy of Science

Alzheimer’s Disease (AD), a prominent neurodegenerative disease (NDD), is the seventh leading cause of death in the United States; however, little is known about prevention. As a result, polyphenols (plant-based compounds) and other natural substances have been studied for their therapeutic potential in improving cognition and memory in AD cases. While the impacts of each polyphenol (phenolic acids, flavonoids, stilbenes, and lignans) contain fundamental similarities, each varies slightly in its impacts on the brain.The purpose of this study was to examine the impacts of polyphenol subgroups on associative memory in AD, tested by building an olfactory association, through the use …

The Effect Of Rosmarinus Officinalis And Trachyspermum Ammi On The Growth Of Eruca Vesicaria Ssp. Sativa In Hydroponics Versus Natural Soil, Emna Guedhami

Journal of the South Carolina Academy of Science

Despite technology being developed to address the food shortages due to increasing population, current methods of cultivating crops are not sufficient. Scientists are addressing this issue by researching biostimulants that stimulate plant growth without harming the environment and researching the use of hydroponics, a way of growing crops without soil. The purpose of this study was to combine the use of essential oils and growing methods (hydroponics and soil) and measure its impact on the height of E. sativa. It was hypothesized that if E. sativa was grown hydroponically with added Rosmarinus officinalis (REO) and Trachyspermum ammi (TEO) over time, …

Table Of Contents

Journal of the South Carolina Academy of Science

No abstract provided.

The Comparative Impact Of Acesulfame-Potassium, Stevia Extract, And Monk Fruit On The Lifespan Of Drosophila Melanogaster, Beverly Holton

Journal of the South Carolina Academy of Science

Non-nutritive sweeteners (NNS), non-caloric sweetening devices, have been widely commercialized to reduce sugar consumption. This intent has associated NNS with health benefits, though reports have correlated the consumption of these substitutes with noncommunicable diseases. The interaction between NNS and distinct physiological processes has been examined, though research into the broader implications of these interactions, toward longevity, for instance, is lacking. The purpose of this study was to compare the impact of three recently FDA-approved NNS - acesulfame-Potassium (ace-K), stevia, and monk fruit - on the survival of Drosophila melanogaster, a model species for longevity research, to discern the possible impacts …

The Effect Of Crystalline Phase On The Toxicity Of Tio2 Nanoparticles To Chlorella, Jisoo Lee

Journal of the South Carolina Academy of Science

Nanoparticles (NPs), or particles with dimensions that go below 100 nanometers, can be released into the environment in various ways, such as through cosmetic products like sunscreen. Titanium dioxide (TiO₂) is a commonly used nanoparticle in sunscreen, and it has the potential to be harmful to various aquatic organisms including algae. The purpose of this study was to examine how the crystalline phase of TiO₂ NPs (brookite or rutile) influenced their toxicity to the microalgae species Chlorella. It was hypothesized that brookite phase TiO₂ NPs would inhibit the growth of Chlorella more than rutile phase TiO …

The Effect Of The Addition Of Carrot Seed Oil And Raspberry Seed Oil On The Uv Intensity Of A Sunscreen, Isra Siddique

Journal of the South Carolina Academy of Science

With continuous developments in research indicating the potential health risks of chemical sunscreen filters, the novel approach of incorporating natural oils with UV light blocking properties has been explored. Natural oils extracted from fruit and vegetables have been proven to possess photoprotective properties, but their effectiveness in combination with synthetic sunscreen filters has not been as thoroughly investigated. The purpose of this study was to determine whether the addition of carrot and raspberry seed oil to an SPF 30 sunscreen would decrease the UV intensity of UVB light passing through the sunscreen and oil mixture. It was hypothesized that if …

Ecological Diversification In An Adaptive Radiation Of Plants: The Role Of De Novo Mutation And Introgression, Benjamin Stone, Carolyn A. Wessinger

Faculty Publications

Adaptive radiations are characterized by rapid ecological diversification and speciation events, leading to fuzzy species boundaries between ecologically differentiated species. Adaptive radiations are therefore key systems for understanding how species are formed and maintained, including the role of de novo mutations versus preexisting variation in ecological adaptation and the genome-wide consequences of hybridization events. For example, adaptive introgression, where beneficial alleles are transferred between lineages through hybridization, may fuel diversification in adaptive radiations and facilitate adaptation to new environments. In this study, we employed whole-genome resequencing data to investigate the evolutionary origin of hummingbird-pollinated flowers and to characterize genome-wide patterns …

Identification Of Regulatory Elements In The Untranslated Regions Of Streptolysin S Associated Gene A Messenger Rna From Group A Streptococcus, Cameron R. Carroll, Sara G. Nibar, Alexis S. Brown, Lauren R. Angello, Gabriela C. Pérez-Alvarado, Brian M. Lee

Journal of the South Carolina Academy of Science

Streptococcus pyogenes, also known as group A Streptococcus (GAS), is a human pathogen associated with a variety of diseases such as strep throat, scarlet fever, toxic shock syndrome, and necrotizing fasciitis. One of the virulence factors released by GAS during an invasive infection is a cytotoxic peptide, streptolysin S (SLS), which inhibits the immune response to necrotizing fasciitis. The streptolysin S associated gene A product, SagA, is modified to produce SLS. Thesag operon includes sagA and the genes required for enzyme-mediated post-translational modifications of SagA and the export of SLS. The sagA gene is contained within the pleiotropic …

Master’S-Level Professional Athletic Training Program Admissions Data: Does It Matter If Your Institution Is Public Or Private?, J M. Cavallario, C Starkey, C E. Welch Bacon

Clinical Practice in Athletic Training

Introduction: Recently, there has been speculation that the transition to a master’s degree for entry into the athletic training profession has resulted in a shortage of athletic trainers (ATs) relative to the number of jobs available. The Board of Certification has announced that the total number of credentialed ATs has not decreased, yet athletic training programs consistently report challenges in recruiting athletic training students to their programs. Data provided by the CAATE from annual reports is typically two years old once it is made publicly available. Program and institutional administrators need access to real-time data to make decisions regarding program …

Employers’ Perceptions Of And Experiences Working With Residency-Trained Athletic Trainers, C E. Welch Bacon, J M. Cavallario, L E. Eberman, J J. Wetherington, F Q. Pecha, B L. Van Lunen

Clinical Practice in Athletic Training

Context: Athletic trainers (ATs) who complete accredited residency training in a specialty area are considered clinical specialists, yet more information is needed to determine whether they are practicing within the higher capacity expected of a specialist. While some quantitative evidence demonstrating the value of residency-trained ATs on patient satisfaction, patient throughput, and the financial impact specific to the physician practice setting exists, residency training is not limited to only ATs who want to provide services in physician practice. Moreover, as accredited athletic training residency programs continue to emerge, it is increasingly important to understand key stakeholders’ perceptions of residency-trained ATs …

Physical Activity, Anxiety, Depression, And Coping In Turkish Men And Women During The First Wave Of Covid-19, Bijen Filiz, Funda Coşkun Özyol, Bengü Güven, Ezel Nur Korur, Yılmaz Yüksel, Cavit Işık Yavuz, Kele Ding, Jingzhen Yang, J. Larry Durstine Phd, Facsm, Faacvpr, Fnak, Ming-Kai Chin, Gıyasettin Demirhan

Faculty Publications

A need exists to better understand the relationships between COVID-19, coping behaviors, physical activity and stress, and COVID-19’s impact on way of life. A cross-sectional study design was used to examine adult physical activity, hope, depression, anxiety, and coping status by gender during the COVID-19 pandemic, and to determine the impact of these variables on the coping process. The study also examined the effect of gender on the relation between physical activity and dependent variables. A global survey instrument was used in this study, including 1,400 Turkish adults. This study identified significant gender-based differences regarding physical activity, hope, depression, anxiety, …

The Multifunctional Catalytic Hemoglobin Fromamphitrite Ornata: Protocols On Isolation, Taxonomic Identification, Protein Extraction, Purification, And Characterization, Anna L. Husted, Lauren A. Presnar, R. Kevin Blackburn, Joseph L. Staton, Stephen A. Borgiani, Edward L. D'Antonio

Natural Sciences Faculty Publications

The multifunctional catalytic hemoglobin from the terebellid polychaete Amphitrite ornata, also named dehaloperoxidase (AoDHP), utilizes the typical oxygen transport function in addition to four observed activities involved in substrate oxidation. The multifunctional ability of AoDHP is presently a rare observation, and there exists a limitation for how novel dehaloperoxidases can be identified from macrobenthic infauna. In order to discover more infaunal DHP-bearing candidates, we have devised a facilitated method for an accurate taxonomic identification that places visual and molecular taxonomic approaches in parallel. Traditional visual taxonomic species identification by the non-specialist, at least for A. ornata or even for other …

The Effects Of A Brand-Specific, Hemp-Derived Cannabidiol Product On Physiological, Biochemical, And Psychometric Outcomes In Healthy Adults: A Double-Blind, Randomized Clinical Trial, Gianna F. Mastrofini, Bridget A. Mcfadden, Alexa J. Chandler Ph.D., Blaine S. Lints, Harry P. Cintineo, Nathaniel D. Rhoades, Caroline S. Vincenty, Sten O. Stray-Gundersen Ph.D., Abbi D. Lane, Shawn M. Arent Ph.D., Cscs*D, Fissn, Facsm, Fnak

Faculty Publications

Background: Cannabidiol (CBD) is a non-psychoactive phyto-cannabinoid derived from the Cannabis sativa plant. CBD exhibits various interactions at receptor sites, prompting the research of its potential anti-inflammatory, immunomodulatory, psychological, and pain-relieving effects. This study aimed to investigate the physiological, biochemical, and psychometric effects of a brand-specific, hemp-derived CBD product in healthy adults over a 12-week observation period. Methods: 54 healthy males and females (age = 25 ± 7y; BMI = 24.82 ± 3.25 kg/m2) recruited from a large Southeastern University completed the study. Participants arrived at the laboratory after > 8 h of fasting, and > 48 h without alcohol consumption and …

Common Questions And Misconceptions About Caffeine Supplementation: What Does The Scientific Evidence Really Show?, Jose Antonio, Daniel E. Newmire, Jeffrey R. Stout, Brandi Antonio, Maureen Gibbons, Lonnie M. Lowery, Joseph Harper, Darryn Willoughby, Cassandra Evans, Dawn Anderson, Erica Goldstein, Jose Rojas, Matías Monsalves-Álvarez, Scott C. Forbes, Jose Gomez Lopez, Tim Ziegenfuss, Blake D. Moulding, Darren Candow, Michael Sagner, Shawn M. Arent Ph.D., Cscs*D, Fissn, Facsm, Fnak

Faculty Publications

Caffeine is a popular ergogenic aid that has a plethora of evidence highlighting its positive effects. A Google Scholar search using the keywords “caffeine” and “exercise” yields over 200,000 results, emphasizing the extensive research on this topic. However, despite the vast amount of available data, it is intriguing that uncertainties persist regarding the effectiveness and safety of caffeine. These include but are not limited to: 1. Does caffeine dehydrate you at rest? 2. Does caffeine dehydrate you during exercise? 3. Does caffeine promote the loss of body fat? 4. Does habitual caffeine consumption influence the performance response to acute caffeine …

Digital Health Care: Time For A New Standard?, Z K. Winkelmann

Clinical Practice in Athletic Training

Introduction: The rapid influx of digitalization in healthcare has presented opportunities for innovative engagement, delivery, and outcomes for patient care. However, it also presents with several barriers and challenges that require teaching and learning opportunities to prepare future athletic trainers for these tasks. Currently, the CAATE curricular content standards integrate digital health care through Standard 64 on health informatics and Standard 87 on biometrics. However, the art and science of digital healthcare are expansive, including various skills such as digital literacy, computer skills, online communication, and privacy and security standards. Methods: The presentation will provide an overview of current digital …

Burnout In Athletic Training: Measured By Survey In Most Common Settings For Athletic Trainers, R Johnson Krug, N Sand, S Brooks, T Smith

Clinical Practice in Athletic Training

Introduction: Burnout is a common problem in athletic training, affecting both athletic trainers (ATs) and the athletes they serve. Athletic trainers are responsible for the prevention, diagnosis, and treatment of sports-related injuries. Long work hours, often including evenings and weekends, as well as frequently under pressure to meet the demands of athletes, coaches, and parents. Athletic trainers face numerous stressors that contribute to burnout. These stressors may include heavy workloads, lack of control over their schedules, time pressures, and pressure to produce positive outcomes. Athletic trainers may also experience emotional and physical exhaustion, depersonalization, and a reduced sense of personal …

Educational Outcomes Of The Dat: A Document Review And Thematic Analysis, S H. Orr, L E. Eberman, M J. Rivera

Clinical Practice in Athletic Training

Context: The transition in athletic training education allows for unique and new opportunities in postprofessional education. The Doctor of Athletic Training (DAT) degree is one of the postprofessional education pathways that could provide athletic trainers advancement in clinical practice, practice leadership, and scholarship. However, there is no data on the educational outcomes of DAT programs and what graduates should expect from their experiences. The aim of this study was to explore whether DAT programs are delivering curricula consistent with the development of clinical scholars with advanced knowledge and skills that are needed for high-level patient care and literature relative to …

Athletic Trainers’ Familiarity With Potential Concussion Biomarkers: A Descriptive Study, T R. Campbell, B J. Davis, J C. Martinez, J M. Cavallario

Clinical Practice in Athletic Training

Introduction: An estimated 3.8 million individuals sustain a concussion annually in the United States. Despite the high number of cases, this number may not accurately depict the true incidence rate due to underreporting of symptoms by patients. With current concussion tools relying heavily on subjective input from patients, it is imperative that a valid and reliable objective tool, such as a clinical biomarker, is identified to improve concussion diagnosis and management. If such a biomarker is identified, it will be essential that athletic trainers (ATs) be familiar with how biomarkers could be used. The purpose of this study was to …

Athletic Training Preceptors’ Experience With Interprofessional Education And Collaborative Practice In The Clinical Learning Environment, A Bender

Clinical Practice in Athletic Training

Introduction: Professional athletic training (AT) programs are now required to align their educational curricula with the Institute of Medicine's (IOM) core competencies, preparing students to work in dynamic, interprofessional healthcare teams to strategically address patient outcomes. Although interprofessional education and collaborative practice (IPECP) is commonly implemented in didactic settings, our understanding of how IPECP is implemented by AT preceptors in various clinical settings is limited. The purpose of this study was to gain a greater understanding of AT preceptors’ experience with IPECP and how they implement this with students in their clinical settings. Methods: A convergent mixed-methods design was used …

Using Alums Involvement To Increase Student Investment In An Athletic Training Program, C J. Heffner, A L. Green

Clinical Practice in Athletic Training

Introduction: Student investment and retention are not new concerns among athletic training program directors. Graduate programs are expected to meet minimum enrollment standards to demonstrate the need for the program. Programs that are unable to maintain student enrollment will be eliminated by institutions experiencing financial stress. Retention strategies and benefits have previously been investigated among undergraduate students and across disciplines. One common theme in the research is that student retention increases when students feel a sense of belonging from the faculty, staff, and peers in their program. There is less research on the retention of graduate students, but the themes …

Using Data In The Classroom To Drive Instructional Design And Delivery, L E. Eberman, J P. Young

Clinical Practice in Athletic Training

Introduction: Roughly half of ATP program directors include education on transgender health care in their programs, and even fewer report having the competence to do so. However, professional-level accreditation standards (56, 57, and DEI 2) require that programs have faculty with contemporary expertise to educate students on these outcomes. Current research suggests a comprehensive transgender and sexual health curriculum, including safe-zone training, traditional lecture, lab, and project-based instruction, was partially effective at changing students’ attitudes toward transgender patients but did not improve sexual health care delivery during a standardized patient encounter. The purpose of this project was to evaluate the …

Traveling The Pathway To A Culturally Competent Health Care Provider: Incorporating Study Abroad Into Your Curriculum, A L. Johnston Green, C J. Heffner

Clinical Practice in Athletic Training

Introduction: As athletic training employment settings expand and diversify, so does the patient population with whom these healthcare providers interact. This growth furthers the need to ensure that students enrolled in an athletic training program (ATP) accredited by the Commission on Accreditation of Athletic Training Education (CAATE) are educated to provide culturally competent care. Culturally competent care is provided when decisions made diminish the inequalities that may be present in a system based upon a patient’s individual circumstances. Implementing cultural competency education may be integrated into athletic training education in various manners as necessitated by each institution’s unique circumstances. Programs …