Life Sciences Commons^™

2010 Adaptive Management Report, Desert Conservation Program

Publications (C)

The Desert Conservation Program administers the incidental take permit issued in 2001 by the United States Fish and Wildlife Service in accordance with the Endangered Species Act. Compliance with the permit requires implementation of the Clark County Multiple Species Habitat Conservation Plan. This plan requires an adaptive management program and a biennial report by an independent science advisor to assess the status of the plan and make recommendations for future funding. This 2010 Adaptive Management Report reviews current data on land use trends, habitat loss, species status and plan implementation, and makes recommendations for the future.

Mimosa: A System For Minimotif Annotation, Jay Vyas, Ronald J. Nowling, Thomas Meusburger, David P. Sargeant, Krishna Kadaveru, Michael R. Gryk, Vamsi Kundeti, Sanguthevar Rajasekaran, Martin Schiller

Life Sciences Faculty Research

BACKGROUND:

Minimotifs are short peptide sequences within one protein, which are recognized by other proteins or molecules. While there are now several minimotif databases, they are incomplete. There are reports of many minimotifs in the primary literature, which have yet to be annotated, while entirely novel minimotifs continue to be published on a weekly basis. Our recently proposed function and sequence syntax for minimotifs enables us to build a general tool that will facilitate structured annotation and management of minimotif data from the biomedical literature.

RESULTS:

We have built the MimoSA application for minimotif annotation. The application supports management of …

Antimicrobial Activity Of D-Lenolate®, Andy Phui

UNLV Theses, Dissertations, Professional Papers, and Capstones

Olive trees are one of the most important fruit trees in the Mediterranean. Although not validated by research, olive leaves are traditionally believed to fight off fever and infections. It has been shown that olive leaf extracts possess antimicrobial activity. Olive leaf extracts contain polyphenols. One of the major phenolic compounds is oleuropein. Oleuropein and other polyphenols have been shown to exhibit antimicrobial activity. East Park Research (EPR) developed an extraction process that they claim does not alter the chemical composition of the olive leaves. The extract is known by the commercial name d-lenolate®. Studies have provided evidence that d-lenolate®, …

Bacillus Cereus And Bacillus Anthracis Germination Kinetics: A Michaelis-Menten Approach, Helen Luu

UNLV Theses, Dissertations, Professional Papers, and Capstones

Bacillus species are rod-shaped, gram-positive bacteria that are capable of producing endospores. In this dormant stage, the endospores can persist in hostile physical and chemical environments. Once conditions become favorable, the spores germinate into actively dividing cells, vegetative cells. Germination is a crucial step for the pathogenicity of the Bacilli in affecting a host organism.

Our study applies mathematical approaches to spore germination to determine whether the binding of one germinant will affect the binding of another germinant. We pursued this approach with two different species, B. cereus and B. anthracis, both pathogenic organisms. B. cereus is a widely known …

Filtration And Growth Rate Of Lake Mead Quagga Mussels (Dreissena Bugensis) In Laboratory Studies And Analyses Of Bioaccumulation, Carolyn Louise Link

UNLV Theses, Dissertations, Professional Papers, and Capstones

In January of 2007, Quagga Mussels (Dreissena bugensis) were identified in Lake Mead, Nevada. An aquatic invasive species, these mussels can significantly alter ecosystems. This study sought to quantify three ecological traits of the species through a series of laboratory experiments and analyses, providing information both for comparison with Dreissena in other locations, as well as for limnologic management decisions. Filtration rate of quagga mussels was quantified using algal strains and natural seston. Two strains of green algae, Nannochloris and Scenedesmus were used to determine mussel filtration rates with a spectrophotometer. Quagga filtration rates of collected Lake Mead seston were …

Evaluation Of A 3-Dimensional Inertial Tracking System For Quantifying Human Movement, David T. Delion

UNLV Theses, Dissertations, Professional Papers, and Capstones

The purpose of this study was to evaluate an inertial tracking unit (ITU) by comparing it to an optical tracking system. The ITU was attached to a bowling ball along with reflective markers for the optical system. Each trial started with the ball at rest on a pedestal the ball was then hung from a steel cable 10 feet long and set in motion in an elliptical pattern for approximately 25 seconds. The ball was then removed from the cable and returned to the pedestal so as to end each trial at rest. This was repeated 10 times with 5 …

A Longitudinal Study Of Neurocognitive Deficits And Functional Outcome In Bipolar Disorder, Brian D. Leany

UNLV Theses, Dissertations, Professional Papers, and Capstones

Bipolar disorder is an affective disorder that, in addition to being characterized by depressive and expansive mood symptoms, often presents with neuropsychological deficits. Bipolar disorder not only impairs an individual’s cognitive abilities, but these cognitive impairments may also impact day-to-day activities causing functional impairment. In other psychiatric disorders such as schizophrenia, it has been shown that the neuropsychological deficits are predictive of poor, long term treatment outcome and functioning. However, while bipolar disorder affects nearly 1 - 2% of the U.S. population (Keck, McElroy, & Arnold, 2001), little is known about the extent that neurocognitive deficits may play in the …

Genes Involved In Mushroom Body Development And Behavior In Drosophila, Christine Nicole Serway

UNLV Theses, Dissertations, Professional Papers, and Capstones

Mushroom bodies (MBs) are the site of multi modal sensory integration critical for associative conditioning in Drosophila. They have been central to research on the structure function relationship in the brain for over one hundred years due to their unique shape and readily accessible physiology. This dissertation incorporates three different approaches to further elucidate the genetic and molecular nature of this structure function relationship.

First, the suite of genetic and molecular tools available in Drosophila melanogaster, facilitated the molecular mapping of a 25-year old MB structural mutant called mushroom body miniature B (mbmB) to the gene Pendulin [Pen, also known …

The Role Of Ecdysone Signaling In Fat-Body Tissue Remodeling And Pupal Metabolism, Nichole Dinell Bond

UNLV Theses, Dissertations, Professional Papers, and Capstones

Holometabolous insects undergo an astonishing transition during their development. During metamorphosis, the larva dramatically changes form and becomes an adult fly. During this process obsolete larval tissues must be eliminated, while tissues required for further development are retained and often remodeled to meet the needs of the adult fly. Tissue remodeling is characterized by morphological changes of the cells in a tissue mass. In many cases, remodeling is characterized by dissociation of the tissue mass, releasing cells to move freely around the body cavity. This process is also common in wound healing and is a key feature of human disease …

Phylogeography Of Two Closely Related Anurans, The Relict Leopard Frog (Rana Onca) And Lowland Leopard Frog (Rana Yavapaiensis), Viktoria Hemmings

UNLV Theses, Dissertations, Professional Papers, and Capstones

I investigate the phylogeography of the relict and lowland leopard frogs (Rana onca; R. yavapaiensis) inhabiting the Mojave and Sonoran deserts. In Chapter 1, I summarize literature describing taxonomy, phylogenetics, and the possible effects of Quaternary climate change on distribution. Examples of phylogeographic patterns from generally co-distributed organisms are provided to develop background for interpreting the structure. In Chapter 2, I investigate the phylogeography of these frogs using mitochondrial DNA data. The analysis supports a previously determined phylogenetic break between taxa however further dividing R. yavapaiensis into two lineages. I estimate a possible Early Pleistocene divergence of R. onca and …

Use Of An Animal Model To Explore Prenatal Predictors Of Insulin And Glucose Metabolism In Southwestern Alaskan Yupiit, Julie Jo Kachinski

UNLV Theses, Dissertations, Professional Papers, and Capstones

Compared to other North American indigenous populations, Southwest Alaskan Yupiit exhibit very low rates of type 2 diabetes despite the occurrence of common risk factors. Contemporary Yupiit obtain a substantial portion of their calories from traditional foods, which contain high amounts of omega-3 polyunsaturated fatty acids. Epidemiological and experimental animal research has linked glucose and insulin homeostasis with a diet high in omega-3s. This study used an experimental animal model to explore potential diabetes protective effects (for adult offspring) of prenatal maternal nutrition modeled on traditional locally-obtained Yupiit diets. The results of this study showed that the adult offspring whose …

An Evaluation Of Lead In Imported Candies Within Clark County, Nevada, Usa, Lee Reuben Ibarra

UNLV Theses, Dissertations, Professional Papers, and Capstones

Imported candies from Latin America pose a danger to children because of the potential for those candies to contain harmful levels of lead. This study sought to identify characteristics of imported candies that typically display higher concentrations of lead. This study also explored the Integrated Exposure Uptake Biokinetic model developed by the EPA. Using lead exposure data specific to Clark County, Nevada, the IEUBK model was used to estimate the amounts of candy a child would have to consume in order to raise his blood lead level to over 10µg/dL (the federal allowable limit).

The results showed that candies with …

The Effects Of Observation, Dialogue, And Attentional Focus In Dyadic Training Protocol, Carolina Granados

UNLV Theses, Dissertations, Professional Papers, and Capstones

The present study examined the impact that dyad training with an emphasis on attentional focus has on learning in the performance of a golf putt. The participants were randomly divided into four dyad groups: dialogue and observation with external focus, dialogue and observation with internal focus, dialogue and no observation with external focus, and dialogue and no observation with internal focus. Participants were given a set of instructions to verbalize to their partners during practice. These instructions were designed to generate either an internal or an external focus. The results determined that those participants who were instructed to have an …

Association Of Body Mass Index, Fruit And Vegetable Intake, And Acculturation In A Sample Las Vegas Hispanic Population, Anne L. Bolstad

UNLV Theses, Dissertations, Professional Papers, and Capstones

The U.S. is experiencing a rising prevalence of overweightedness that has been identified as the second leading cause for chronic health conditions threatening public health. Overweightedness has grown disproportionately among ethnic sub-groups. In the fastest going minority population in the U.S., Hispanic Americans are observed with disparately high body mass index, placing them at heightened risk for poor health outcomes. Research suggests five servings of fruit and vegetables, in any combination, provides a sound nutritional base for healthful living and helps to maintain normal body weight.

Americans are known to have poor eating habits while foreign-born populations have well balanced …

Kinetics At Front Foot Contact Of Cricket Bowling During A 10-Over Spell, Jacobus Noël Liebenberg

UNLV Theses, Dissertations, Professional Papers, and Capstones

The purpose of this study was to determine what effect bowling a 10 over spell (60 balls) would have on approach velocity, vertical ground reaction forces and shock attenuation during the front foot contact of a delivery stride in cricket.

Ten Amateur cricket players (age 27±4 years, height 1.78±0.3 m, mass 80.6±8.5 kg) participated in the study. Testing was conducted at University of Nevada, Las Vegas in the Biomechanics laboratory. Participants performed a 10-over bowling spell from a 12 meter run-up. These dependent variables were measured and calculated during the bowling protocol: 1) approach velocity 2) vertical ground reaction force …

The Relationship Of A Long-Term Exercise Program And Selected Coronary Risk Factors, Maryse Wells

UNLV Theses, Dissertations, Professional Papers, and Capstones

The purpose of this study was to investigate body composition and blood pressure as potential risk factors for coronary artery disease after 3 years of participation in a structured exercise program. The sample group was comprised of 21 male participants (66%) and 11 (34%) female participants with a mean age of 55 years who attended the exercise program and completed health and fitness testing annually. Participants did cardiovascular, strength, and flexibility training 5 days a week with an average attendance of 70%. After 3 years, BMI, waist circumference, and percent body fat increased significantly (p < .01); blood pressure levels did not change significantly (p > .05). Low control of diet …

Transforming Space Into Place: Development, Rock Climbing, And Interpretation In Red Rock Canyon National Conservation Area, 1960-2010, Megan Sharp Weatherly

UNLV Theses, Dissertations, Professional Papers, and Capstones

Though Americans tend to view wilderness as separate from nature, environmental historians have argued that wilderness is a cultural construct more than a quantifiable geographic category. Red Rock Canyon National Conservation Area (NCA), a 195,000-acre tract located west of Las Vegas, Nevada, is one such cultural construction. Since 1960, this BLM-managed parcel has served as a local and regional expression of broader, national trends in outdoor recreation, interpretation, and development and thereby forced visitors to engage (often unknowingly) in a cultural dialogue about consumerism, technology, and identity. With information from newspapers, archival collections, oral histories, and government documents, this thesis …

Effects Of Obesity On The Biomechanics Of Children’S Gait At Different Speeds, Philana-Lee Gouws

UNLV Theses, Dissertations, Professional Papers, and Capstones

The purpose of the study was to investigate the relationship between body mass index (BMI) and spatio-temporal gait characteristics of overweight/obese and non-obese school-aged children (12-14 years) at two different walking speeds. Eighty-four overweight/obese (n=28; age: 13.96 ± 0.79 yrs; mass: 74.8 ± 18.21 kg; height: 159.2 ± 7.1 cm and BMI: 29.28 ± 5.64 kg/m2) and non-obese students (n=56; age: 13.72 ± 0.79 yrs; mass: 51.7 ± 10.2 kg; height: 157.8 ± 8.3 cm and BMI: 20.69 ± 2.74 kg/m2) with no present injuries were recruited. Participants were instructed to walk across an electronic walkway in each of two …

Enhanced Food Marketing To Children On The Internet: A Content Analysis, Sarah A. Ochsner

UNLV Theses, Dissertations, Professional Papers, and Capstones

In light of the continuing epidemic of childhood obesity, aggressive food marketing strategies have come under increased scrutiny as a possible contributing factor. It has been acknowledged in numerous studies, that poor nutrient quality of food and beverages dominate children's programming. The growth in child-specific media envoys has further increased favorable opportunities to market food and beverages to children, notably less regulated and parentally unsupervised. According to the U.S. Census Bureau, 65% of children aged 6-11 have home access to the Internet; and today, the majority of food manufacturers operate websites appealing to children. The Institute of Medicine issued a …

Joint Fire Science Program – Lake Mead National Recreation Area Revegetating Burned Arid Lands: Identifying Successful Native Species Using Trait And Competition Analysis: Quarterly Progress Report, Time Period: January 1 — April 30, 2010, Margaret N. Rees

Fire Science

• Article entitled “Competitive Hierarchy of Native Desert Plants with Red Brome (Bromus rubens): Towards Identifying Invasion-Reducing Species" was submitted to the Invasive Plant Science and Management journal
• Maintained nursery plots and added installment of nitrogen treatment.
• Took measurements in nursery (competition) plots and harvested biomass.

Partitioning Of Minimotifs Based On Function With Improved Prediction Accuracy, Sanguthevar Rajasekaran, Tian Mi, Jerlin Camilus Merlin, Aaron Oommen, Patrick R. Gradie, Martin R. Schiller

Life Sciences Faculty Research

Background

Minimotifs are short contiguous peptide sequences in proteins that are known to have a function in at least one other protein. One of the principal limitations in minimotif prediction is that false positives limit the usefulness of this approach. As a step toward resolving this problem we have built, implemented, and tested a new data-driven algorithm that reduces false-positive predictions.

Methodology/Principal Findings

Certain domains and minimotifs are known to be strongly associated with a known cellular process or molecular function. Therefore, we hypothesized that by restricting minimotif predictions to those where the minimotif containing protein and target protein have …

Assessing Invasive Plant Species As Threats In Mojave Desert Parks, Sara L. Mcpherson, Donovan J. Craig, Scott R. Abella

Graduate Research Symposium (2010 - 2017)

The invasion and persistence of exotic plant species threatens the natural features that national parks are designed to protect. For example, park managers have witnessed an increase in the frequency, extent and intensity of fires along with a reduction in native species richness and diversity. Many park managers are familiar with a suite of highly invasive plants, but lack a comprehensive and systematic way of prioritizing invasive plant species based on potential threats to the parks’ resources. We have entered into a collaborative project with the National Park Service’s Inventory and Monitoring program to develop an invasive plant early detection …

29 Years Of Vegetation Community Change Across Environmental Gradients In A Mojave Desert Mountain Range, Christopher L. Roberts, James S. Holland, Scott R. Abella

Graduate Research Symposium (2010 - 2017)

There is a great deal of uncertainty as to how biological communities respond to changes in land use and climate change, a situation particularly relevant in protected areas such as national parks that were designated to conserve specific biological features. Utilizing extant vegetation data sets with repeatable methodology can provide opportunities for insight into previous vegetation change and provide base line data for long-term monitoring projects useful for modeling vegetation community trajectories. We have relocated and resurveyed 106 sites from a vegetation community study initiated in 1979 in the Newberry Mountains, southern Nevada, within Lake Mead National Recreation Area managed …

Mojave Applied Ecology Notes Spring 2010, Anna San, Donovan J. Craig, Lindsay P. Chiquoine, Scott R. Abella, E. Cayenne Engel, Christina L. Lund, Jessica E. Spencer, Edward P. Weber Ph.D, Jill E. Craig

Mojave Applied Ecology Notes

Seed removal rates of Sahara mustard by rodents and ants, Mojave Desert Network exotic invasive inventory, gypsum roadside disturbance restoration update, new paper out on post-fire plant establishment, UNLV establishes school of environmental and public affairs

An Analysis Of Restaurant Food Safety Violations: Human Factors, Non-Human Factors, And Food-Borne Illness, Jai Choung

UNLV Theses, Dissertations, Professional Papers, and Capstones

An estimated 76 million food-borne illnesses occur in the United States each year, causing 325,000 hospitalizations and 5,000 deaths, according to the U.S. Food and Drug Administration. Food safety is an increasingly important topic to governmental food regulators, not only in trying to reduce the number of illnesses from contaminated food. Governmental regulators also are becoming aware of the vulnerability of the food supply chain as a target for bioterrorism. Recommendations have been developed to protect the U. S. food supply from terrorism, but little of the research and recommendations relate to the food-service level.

Eighty percent of reported cases …

From The Valleys To The Mountains: The Biographic History Of Antelope Squirrels, Bats, And Chipmunks In Western North America, Stacy James Mantooth

UNLV Theses, Dissertations, Professional Papers, and Capstones

Genetic differentiation within and between species often coincides with significant geological or climatic changes that have shaped the sizes and locations of their geographic ranges and altered the connectivity between populations over time. Across western North America, many endemic taxa experienced high levels of initial divergence associated with geological transformations of the Neogene, with subsequent diversification and geographic structuring of populations associated with climatic changes during the Quaternary. As such, we can use a combination of molecular markers and genetic analyses to effectively examine the evolutionary and biogeographic histories of populations, species, and regional biotas whose signatures of differentiation are …

Phylogeography And Landscape Genetics Of The Flammulated Owl: Evolutionary History Reconstruction And Metapopulation Dynamics, Markus Mika

UNLV Theses, Dissertations, Professional Papers, and Capstones

Taxa occur across ever changing landscapes on different spatial and temporal scales. Choosing the appropriate scale for collecting data and drawing inferences is critical for understanding the history of a species and its populations. Here, I describe research in which I investigated phylogenetic patterns and population genetics for Flammulated Owls (Otus flammeolus) representing 14 localities from throughout the species' distribution, and compared regional landscape features with the distribution of genetic diversity. This small, insectivorous owl migratory (Family Strigidae) breeds in western conifer and deciduous dry forests of the mountains from southern Mexico to British Columbia. Sedentary populations are found in …

Space Use And Mating Activities In The Speckled Rattlesnake, Xavier Glaudas

UNLV Theses, Dissertations, Professional Papers, and Capstones

Our understanding of space use variation in response to the temporally varying importance of specific resources is poorly understood in reptiles, because spatial studies are rarely placed into an explicit ecological and behavioral context. I examined how space use differed between the mating and post-mating seasons, and how this variation related to three important resources, mating partners, food, and refuge, in an adult population of the speckled rattlesnake Crotalus mitchellii in the Mojave Desert of southwestern North America. During the mating season (late April to early June), Crotalus mitchellii increased distance traveled per unit time, because wide-ranging behavior likely enhances …

The Role Of Transcription In Stationary Phase Mutagenesis In Bacillus Subtilis, Holly Anne Martin

UNLV Theses, Dissertations, Professional Papers, and Capstones

Stationary phase mutagenesis, also known as stress-induced or adaptive mutagenesis, is defined as the accumulation of mutations during conditions of no net growth or conditions of stress. This process has been implicated in acquiring antibiotic resistance and evasion of host immune responses in microbial pathogens and in the generation of mutations that lead to neoplasia in animal cells. Previous work has shown that defects in DNA synthesis and repair systems contribute to the formation of adaptive mutations; however the role of transcription in the accumulation of mutations is still being examined. It is speculated that transcriptional derepression leads to an …

Lake Mead National Recreation Area Monitoring And Evaluation Of Sensitive Wildlife: Quarterly Progress Report, Period Ending March 31, 2010, Margaret N. Rees

Wildlife Monitoring

Project 1. Relict Leopard Frog Monitoring, Management, and Research

• All milestones and deliverables are on schedule
• Translocation program initiated for this year with egg mass collections in early spring
• Tadpole rearing underway, first releases schedule for the end of March 2010
• Diurnal egg mass surveys were completed at all sites
• Nocturnal surveys initiated and in progress
• Continuing mark-recapture efforts at Blue Point Spring initiated for spring season
• Data transferred to County completed

Project 2. Bald Eagle Winter Monitoring and Evaluation

• Project has essentially been completed
• All final report deliverables were completed
• Although not specified in the scope of work for …