Life Sciences Commons^™

Primary Antibody Responses To Thymus-Independent Antigens In The Lungs And Hilar Lymph Nodes Of Mice, S. Niranjan Goud, Alan M. Kaplan, Subbarao Bondada

Sanders-Brown Center on Aging Faculty Publications

B lymphocytes from the pulmonary lymphoid tissues were stimulated with a variety of thymus-independent (TI) antigens by intratracheal (i.t.) immunization. Immune responses in the lungs and hilar lymph nodes (HLN), which are part of the localized lymphoid tissue, as well as in the spleen, the systemic lymphoid organ, were studied. Thus, primary i.t. immunization of mice with the TI-1 antigen trinitrophenyl-lipopolysaccharide (TNP-LPS) elicited both antigen-specific and polyclonal plaque-forming cell responses from HLN, lung, and splenic B lymphocytes. These responses appeared as early as 3 days after immunization and declined by day 7. Similar immunization with another TI-1 antigen, TNP-Brucella …

Effect Of Dietary Aluminum Sulfate On Calcium And Phosphorus Metabolism Of Broiler Chicks, Akmed S. Hussein, Austin H. Cantor, Thomas H. Johnson, Robert A. Yokel

Animal and Food Sciences Faculty Publications

The effect of dietary aluminum sulfate on Ca and P metabolism was studied using 1-day-oldmale broiler chicks. In Experiment 1, practical diets providing .90% Ca plus .45% available P (P_av), .90% Ca plus .78% P_av, 1.80% Ca plus .45% P_av, or 1.80% Ca plus .90% P_av were fed with 0 or .392% Al as aluminum sulfate for 21 days. The control diet (.90% Caplus .45% P_av) without added A1 was fed to all chicks during Days 22 to 49. In general, Al significantly (Pi), tibia breaking strength, tibia weight, percentage of …

Relationship Of Dietary Aluminum, Phosphorus, And Calcium To Phosphorus And Calcium Metabolism And Growth Performance Of Broiler Chicks, Akmed S. Hussein, Austin H. Cantor, Thomas H. Johnson, Robert A. Yokel

Animal and Food Sciences Faculty Publications

Dietary treatments providing three levels of added Al (0, .196, or .392%) as aluminum sulfate and of available phosphorus (P_av) (.45, .68, or .78%) in a factorial arrangement were administered to day-old chicks in Experiment 1. Plasma inorganic phosphorus (P_i) was significantly (P < .05) elevated by increasing P_av and was decreased by Al. Body weight gain, feed intake, and the gain:feed ratio at Day 21 were significantly decreased by increased concentrations of Al, but were unaffected by the P_av concentrations. Decreases of 39 and 73% in weight gain and of 34 and 66% in feed intake resulted from feeding …

Response Of Soybeans To Available Potassium In Three Kentucky Soils, John H. Grove, William O. Thom, Lloyd W. Murdock, James H. Herbek

Agronomy Notes

Soybeans are produced in nearly all of Kentucky's soil physiographic regions. Soybean response to potassium (K) fertilization and soil test Kvaries with soil type and location. This variation may be associated with the wide range in soil chemical and mineralogical properties among the different regions. Of particular interest are differences in that level of soil test Kat which no further yield response to K fertilizer application would be expected. The main objective of this work was to evaluate the soil K-soybean response relationship on three different field sites where soybean yields were increased by fertilizer K. Several measures of soil …

Soil Test Potassium As An Indicator Of Tobacco Response To Band Application Of Fertilizer, J. L. Sims, Kenneth L. Wells, E. C. Schwamberger

Agronomy Notes

During the past 10 years, several studies have been conducted at Lexington on the University of Kentucky Experiment Farm to compare the effect of banding fertilizer 10 to 12 inches to each side of the row with preplant broadcast applications on growth and yield of burley tobacco. Generally, banding most of the nitrogen and potassium has led to fewer problems with salt and manganese toxicity and to improved growth during the early season. However, as compared to broadcast application, banding has had varying effects on cured leaf yields. In 'some experiments banding resulted in higher yields, in other experiments in …

Potential Use Of Ethephon To Control Lodging Of Soybeans, Larry J. Grabau, Robert C. Pearce, J. V. Konsler

Agronomy Notes

Lodging is sometimes a serious problem with soybeans in Kentucky, especially with the full season crop. However, under, excellent growing conditions, or if planting rates are too high, substantial lodging of double crop soybeans can also occur. If lodging occurs early during seed fill, it can reduce yields directly by causing poorer light use and increasing diseases. If lodging occurs late during seed fill, it will have little direct effect on yield, but may have an indirect effect by slowing down harvest and increasing harvest losses. Thus, the use of an inexpensive chemical to reduce soybean lodging is attractive. Our …

Effect Of Banded Fertilizer On Manganese Toxicity Of Burley Tobacco, J. L. Sims, Kenneth L. Wells, E. C. Greer

Agronomy Notes

Relatively large amounts (1500 to 3000 lbs per acre) of inorganic fertilizers are commonly broadcast preplant in the production of burley tobacco. Such high rates of fertilizer increase both the salt content of the soil solution and soil acidity, often causing reduced stands, manganese toxicity, plant molybdenum and calcium deficiencies, delayed growth and maturity, and reduced yield and quality of cured leaf. Manganese toxicity alone is thought to cost growers 30-40 million dollars each year from yield loss and the costs associated with growing extra acreage the following year to make up their quota¥ Improved fertilizer management systems are needed …

Pneumatic Metering Of Hardwood Tree Seeds, Larry G. Wells, T. W. Richards, Timothy D. Smith, George B. Day

Biosystems and Agricultural Engineering Faculty Publications

A commercial, no-till row planter was extensively modified for use in direct seeding of hardwood tree species in surface mine reclamation. A pneumatic seed metering system was designed, fabricated, and mounted on the planter frame.

The metering device was tested under laboratory conditions which simulated varying terrain slope and field speed with seeds of various hardwood tree species. Metering efficiencies of 97% were achieved for pin oak seeds at 3.2 km/hr (2.0 m/h) and 50% slope. For red oak, 97% efficiency was achieved for 50% slope at 4.0 km/h (2.5 mph). Unsatisfactory metering was noted during tests of larger seeds, …

Kentucky Pride Hay Growers Association — After One Year, Mark Mattingly

Kentucky Alfalfa and Stored Forage Conference

High quality forages have been the "frontline" for successful horse and cattle operations for years. In Kentucky, we have grown, harvested and stored hay on the farm for as long as we have farmed. It is one of our principal forms of overwinter feed and has been grown primarily as an on-the-farm feed source.

Drying High Moisture Alfalfa Hay, Michael Collins, Blaine Frank Parker, George M. Turner

Kentucky Alfalfa and Stored Forage Conference

We all recognize the value of alfalfa in horse, dairy and beef rations. That's why we harvest over 17 million acres of this crop in the United States every year. Hay is a good way to harvest alfalfa because it stores well, provides long fiber in rations and we can market the surplus as a cash crop. Higher yields and higher quality mean more profit so we push to be sure we use the best management practices. All too often though, we lose part of all of a crop to rain damage. Some yield and quality is lost due to …

Hay Additive Review: "Where We've Been, Where We're Going", William C. Mahanna, Steve Soderlund

Kentucky Alfalfa and Stored Forage Conference

The losses from harvest to feed bunk are undoubtedly greater with hay than any other crop grown by the American farmer. The economic implications of reducing losses through improved harvest technologies is staggering when one considers the 75-85 million tons of alfalfa harvested out of the total 140-145 million tons of hay produced annually in the United States.

Alfalfa Producers: Do You Really Know How Good Your Alfalfa Is? Forage Analysis: Importance And Interpretation, Jimmy C. Henning

Kentucky Alfalfa and Stored Forage Conference

Forages make up over 75% of the diet of all cattle and are the basis of cow-calf industry in the midwest. However, a recent survey of over 1000 livestock producers in Missouri revealed that only 5.6 % had ever had their hay tested. They gave several reasons for why they had not tested hay (Table 1). By a large margin, the greatest reason for not testing was that they did not see a need. This fact highlights the purpose of this paper and presentation at the 10th Annual Kentucky Alfalfa Conference. This paper will explain the terms used in …

Alfalfa Cutting Management, Garry D. Lacefield

Kentucky Alfalfa and Stored Forage Conference

Alfalfa "Queen of the Forage Crops" is the most important forage legume grown in the united States. It is grown over a wide range of soil and climatic conditions. Alfalfa has the highest yield potential and the highest feeding values of all adapted perennial forage legumes. It is a versatile crop which can be used for pasture, hay, silage, green-chop, soil improvements and human consumption. As a result of this crops many merits; especially yield, quality and versatile use, it can be used successfully in many animal feeding programs.

Developing Alfalfa Varieties For The 21st Century, Jim Moutray

Kentucky Alfalfa and Stored Forage Conference

As we approach the end of the 20th century, alfalfa improvement is happening at a very rapid pace, New varieties are being released at the rate of more than 30 per year. During the past 30 years, the roles of public and private research have changed significantly. Emphasis in public efforts has changed from variety development to basic research accompanied by an overall reduction in funding. Private industry, which released less than 20% of the new varieties prior to 1960, has released over 95% of the varieties since 1981. Funding of private research has increased dramatically during this period.

Foreword [1990], Garry D. Lacefield

Kentucky Alfalfa and Stored Forage Conference

This is the front matter of the proceedings.

The Effect Of Oil Well Brines On Agricultural Fields And Water, V. P. Evangelou, M. Marsi, Kenneth L. Wells

Agronomy Notes

What is Brine and Where Does it Come From?
Brine is the salty water trapped in rock formations associated with oil and gas deposits. It consists mostly of sodium chloride but can also contain other things such as organics, bromide, some heavy metals and boron. Its source as a pollutant is usually oil stripper wells which produce less than 10 barrels of oil per day with typically a 10:1 ratio of brine to oil. Such wells are distributed throughout Kentucky and are often located on farmland. In some cases, brine rises to the land surface even where no oil wells …

Does Chemical Leaf Burn Of Double Crop Soybeans Reduce Yield?, Larry J. Grabau, Todd W. Pfeiffer, S. Nevins

Agronomy Notes

Most farmers who have applied acifluorfen (sold as Blazer or Tackle) for postemergence control of broadleaf weeds in soybean fields are aware that leaf crinkling, bronzing, and necrosis can result. Despite this, studies in Kentucky, Illinois, Arkansas, and South Carolina have shown that full season soybeans have plenty of time to recover from leaf burn caused by this herbicide, and no yield losses have been found. However, double crop soybeans are usually planted much later than the full season crop. This delay in planting may reduce the time for plants to complete vegetative growth before beginning the seed production process. …

Automated Sorting Of Oriented Tobacco Leaves, Larry G. Wells, M. J. Bader

Biosystems and Agricultural Engineering Faculty Publications

A mechanism has been developed which removes cured burley tobacco leaves from plants so that midribs are oriented parallel. A photosensor was utilized to count leaves as they were removed and a microprocessor determined the number of leaves allocated to each of three grades. Experiments indicated that 84% of leaves removed could be correctly sorted with a 2.4% material loss at an effective rate of approximately 2.4 times that of conventional methods.

The Nature And Value Of Residual Soil Fertility, William O. Thom

Agriculture and Natural Resources Publications

What nutrients does a particular soil have before fertilizer is added? To answer this question, agronomists refer to residual soil fertility, the level of available plant nutrients which a soil can provide without additional fertilization.

Managing Diseases Of Alfalfa, Paul C. Vincelli, Garry D. Lacefield, Jimmy C. Henning

Agriculture and Natural Resources Publications

Alfalfa can be a vigorous and productive forage crop for Kentucky farmers. Like all farm crops, however, alfalfa is subject to infectious diseases that can limit forage production. Managing these diseases is an important part of economical alfalfa production.

Alfalfa diseases can cause reduced forage yield, reduced forage quality, and decreased stand persistence. Sometimes, the effects of infectious diseases can be dramatic, such as sudden stand loss in a fall-seeded crop caused by Sclerotinia crown and stem rot. Often, the effects of diseases on alfalfa are more subtle but no less important. For example, alfalfa plants with Phytophthora root rot …

Submitting Plant Specimens For Disease Diagnosis, B. C. Eshenaur, Paul R. Bachi, William C. Nesmith, John R. Hartman, Donald E. Hershman, Paul C. Vincelli, Cheryl A. Kaiser

Agriculture and Natural Resources Publications

Accurate diagnosis of a plant disease depends on having a fresh sample representative of the problem. To get an accurate diagnosis when your plant has a disease problem, you need to collect the right kind of sample.

You also need to provide complete information about the plant and its history. Since the county Extension agent and the person making a diagnosis probably won't see the problem first hand at the growing site, you need to help them create a mental picture of the disease situation. You can provide this help by filling out the information requested on various Plant Disease …

Disease Management In The Home Lawn, Paul C. Vincelli, A. J. Powell Jr.

Agriculture and Natural Resources Publications

Numerous infectious diseases can occur on lawns in Kentucky. Unless diagnosed and managed, these diseases can sometimes cause extensive damage. A sound disease management program benefits your lawn in two ways:

• reduces the severity of lawn diseases
• improves the lawn's recovery should a disease outbreak occur.

This publication describes lawn management practices that can help control diseases of turfgrasses commonly used in home lawns -- Kentucky bluegrass, tall fescue, and perennial ryegrass. You can control diseases of turfgrasses most effectively by using as many of the following lawn management practices as feasible.

The first step in improving your lawn is …

Macrophages But Not B Cells From Aged Mice Are Defective In Stimulating Autoreactive T Cells In Vitro, Aruna Seth, Mitzi Nagarkatti, Prakash S. Nagarkatti, Subbarao Bondada, Venkatachalam Udhayakumar

Microbiology, Immunology, and Molecular Genetics Faculty Publications

In the present study the effect of aging on the capacity of Ia⁺ cells to stimulate autoreactive T cells in the syngeneic mixed lymphocyte reaction (SMLR) was investigated. Using young CD4⁺ T cells as responders, it was observed that unseparated whole spleen cells from aged mice had normal stimulatory activity comparable to that of young spleen cells. Interestingly, however, when purified splenic adherant cells (SAC) enriched for macrophages or splenic B cells were used as stimulators, aged SAC but not aged B cells were found to be defective in stimulating autoreactive T cells. This defect in aged SAC …

Beef Cattle Production On Surface-Mined Land In Central Appalachia, William O. Thom, H. B. Rice, David Adams, Charles May, Curtis Absher

Agriculture and Natural Resources Publications

Extensive acreages of reclaimed surface-mined land in Central Appalachia are currently producing forages that can be used in beef cattle production. During reclamation, after these areas were graded, they were seeded with grass and grass-legume mixtures to form a ground cover and to reduce soil loss. Usually in the past these areas have not been managed to provide forages for beef cattle, but they can economically support beef cattle production when managed correctly.

Phenotypic And Functional Properties Of B Lymphocytes From Aged Mice, Subbarao Bondada, Joan Morris, Richard J. Kryscio

Microbiology, Immunology, and Molecular Genetics Faculty Publications

Phenotypic and functional properties of B lymphocytes from individual young and old mice of different inbred strains were studied. B lymphocyte subpopulation defined by the ratios of the densities of cell surface IgM and IgD were found to be altered with age. However, such alterations in B cell subsets were found only in 30-40% of the old mice. B cell mitogenic responses to anti-μ and anti-Lyb2 antibodies were decreased in a majority of DBA/2 mice. Proliferative responses to LPS and antiμ were reduced only in a minority of CBA/Ca mice but there was a very good correlation in the responsiveness …

Stubble Losses Of Kentucky Soybeans, Larry J. Grabau, Todd W. Pfeiffer

Agronomy Notes

Double crop soybeans in Kentucky tend to be shorter than plants from full season plantings. Since lowest pod height is related to plant height, double crop soybeans may be subject to greater harvest losses due to pods remaining below the level at which the combine header is operated. While it would be possible to lower the header closer to the soil to pick up some of those pods, that would slow down harvest and increase the risk of picking up stones and other trash. Although the actual cutting heights used in Kentucky are not known, some producers use combines with …

Method And Composition For Safely Delaying Parturition And Synchronizing Farrowing In Swine, Lee A. Edgerton, Bruce T. Eckerle

Animal and Food Sciences Faculty Patents

A method and composition are provided for extending the gestation period of a sow so as to increase the live birth and survival rates of a swine litter without adversely affecting the sow. The method includes the step of administering an effective dosage of estrogen to the sow 21/2-7 days prior to the end of the expected gestation period for the sow. The composition administered includes from 0.5-10 mg of an estrogen selected from a group consisting of estradiol benzoate, estradiol valerate, estrone, estradiol 17β and effective mixtures thereof. The composition is adapted for administration parenterally by including a carrier …

Buffalo Clover, Norman L. Taylor, J. N. N. Campbell

Agriculture and Natural Resources Publications

Two clovers native to Kentucky, buffalo clover (Trifolium reflexum) and running buffalo clover (T. stoloniferum) recently have been re-discovered. These clovers were common in Kentucky until about 1850. Their names are derived from the fact that they occurred mostly along buffalo trails through woods. Historical records indicate that buffalo clover occurred on drier and less fertile soils of the former Big Barren Region and the Shawnee Hills, whereas running buffalo clover was widespread on moist, fertile soils in the Bluegrass Region. Both are now extremely rare in Kentucky. At present buffalo clover has been found in Mammoth Cave National Park, …

Some Effects Of Shifting To Conservation Tillage Systems For Intensive Production Of Corn And Soybean, Kenneth L. Wells, H. C. Vaught, David Heisterburg

Agronomy Notes

About three-fourths of Kentucky's cropland base occurs on sloping land with some degree of erosion hazard. During the past decade, production of soybeans and corn increased rapidly in Kentucky, particularly on sloping land. This resulted in severe erosion on many farms, particularly in the "intensive grain producing counties. Much of this field erosion could be greatly reduced by developing a system for each field that would incorporate use of such agronomic practices as sod waterways, no-till planting, contour plowing, minimum tillage, strip cropping, double-cropping, winter cover crops, crop residue management and rotations. It is believed that the current acreage of …

Putting A Top Quality Forage Program Together On Your Farm, Richard Brown Phillips

Kentucky Alfalfa and Stored Forage Conference

You know most of us here, and I am including myself, really don't have a super talent for predicting the future with a great deal of consistency, however, our interest doesn't have to be in predicting, but rather, just primarily in considering potentials and opportunities in the future. What do you say, let's take a little time to look at some of the opportunities in the forage industry. The nation's No. 1 cash crop.