Life Sciences Commons^™

Foreword [1992], Garry D. Lacefield

Kentucky Alfalfa and Stored Forage Conference

This is the front matter of the proceedings.

Corn Response To Zinc On High Ph Soils, William O. Thom, James E. Dollarhide, Jeff Henderson, Vern Case

Agronomy Notes

Corn is the most responsive to zinc of all agronomic crops grown in Kentucky. Zinc deficiency can occur most often in soil of the Inner Bluegrass and in south central Kentucky. This deficiency is most likely when these soils have a high pH, although such factors as high phosphorus, low organic matter, and loss of topsoil through erosion or construction can increase deficiency symptoms. Also, zinc deficiency is more prevalent during a cool, wet spring with symptoms often disappearing when the soils become warmer and drier. Zinc is a very immobile element, thus roots must grow throughout the root zone …

Soil And Water Conservation Provisions Of The 1990 Food, Agriculture, Conservation And Trade Act, Monroe Rasnake, Bob Eddleman, Tommy Howard

Agriculture and Natural Resources Publications

Soil conservation is not new in Kentucky. Most farmers are aware of the need to protect soil and have used practices such as crop rotation, residue management, contour tillage, sod waterways, and no-till for many years. However, soil erosion is of neverending concern and still remains a problem in many areas.

Wobbler Syndrome In Horses, Equine Section, Department Of Animal Sciences, University Of Kentucky

Agriculture and Natural Resources Publications

Wobbler Syndrome refers to a number of disease states in the horse. The most common is termed cervical vertebral malformation (CVM) and is characterized by malformation or compression of the spinal cord which leads to spasticity, ataxia, and incoordination. These symptoms are caused by damage to or compression on the spinal cord.

Cervical vertebral instability (CVI) and cervical static stenosis (CSS) are the two distinct types of CVM. CVI causes dynamic spinal cord compression. It typically affects horses from 4-12 months of age. CSS typically affects horses between 12-36 months of age and is characterized by a closing of the …

Priming Of Peripheral Lymph Node B Cells With Tnp-Ficoll: Role Of Lymphokines In B Cell Differentiation, S. Niranjan Goud, Alan M. Kaplan, Subbarao Bondada

Microbiology, Immunology, and Molecular Genetics Faculty Publications

We have previously shown that peripheral lymph node (PLN) B lymphocytes of adult DBA/ 25 mice failed to make an antibody responseto type 2 antigen TNP-Ficoll, but exhibited a good antibody response to type 1 antigen TNP-BruceNa abortus. In the present study we wanted to find out whether the unresponsivenessof PLN B cells to TNP-Ficoll is due to defectsin the early activation and proliferation stage or in the final differentiation stageof B cells. Therefore, we have used a two-step protocol of in vivo immunization of mice with TNP-Ficoll and the subsequent in vitro challenge with TNP-Brucella abortus and studied the …

Hybrid Variation For Yield, Crude Protein, And Feed Value Of Corn, Z. Hu, C. G. Poneleit, K. O. Evans, Michael Collins, D. O. Liptrap

Agronomy Notes

The value of corn as a feed grain depends on the yield per acre, the protein content of the grain, and for some livestock, the lysine level of the protein. Most farmers are aware of yield differences among hybrid corn varieties but may not realize that protein content can also vary significantly. Grain protein level should be accounted for to determine how much protein supplementation will be needed to balance an appropriate animal diet. However, since corn protein is deficient in the amino acid lysine, which is essential for non-ruminant animals, lysine content as well as crude protein content should …

Transformation, Somatic Embryogenesis And Whole Plant Regeneration Method For Glycine Species, Glenn B. Collins, David Hildebrand, Paul A. Lazzeri, Thomas R. Adams, Wayne A. Parrott, Lynn M. Hartweck

Plant and Soil Sciences Faculty Patents

A method for somatic embryogenesis of soybean, (Glycine max), Glycine soja and other Glycine species is provided using immature cotyledon tissue, preferably with the embryonic axis removed, comprising culturing said tissue on a medium containing auxin, preferably NAA at a concentration of at least about 15 mg/l. A further method for such somatic embryogenesis is provided wherein the culture medium contains a synergistically acting lowered carbohydrate and auxin concentration. Particularly embryogenic cells of such tissue are identified and improved maceration methods for contacting such cells with regeneration and transformation media are disclosed.

Methods for transforming somatic tissue from …

The Tall Fescue Endophyte, Don Ball, Garry D. Lacefield, Carl S. Hoveland

Agriculture and Natural Resources Publications

Tall fescue (Festuca arundinacea Schreb.) is the most important cool-season grass in the United States, providing the primary ground cover on some 35 million acres. It is a versatile perennial used to provide pasture and hay for livestock, for various turf purposes, and for erosion control. Commonly referred to as simply "fescue," this widely adapted, persistent grass is seed propagated, tolerant of a wide range of management regimes, and it produces good forage yields. Laboratory nutritive analyses of fescue compare favorably to those of many other cool-season grasses.

Method Of Reducing Odor Associated With Hexanal Production In Plant Products, David Hildebrand, Thomas R. Kemp, Roger Andersen, John H. Loughrin

Plant and Soil Sciences Faculty Patents

A method of reducing the formation of hexanal by plant parts comprises breaking the plant parts, adding thereto an agent comprising lipoxygenase 3 enzyme and allowing the plant parts to remain in contact with the enzyme under conditions effective to attain the desired effect. A method of producing a low odor plant part meal or flour comprises breaking up the plant parts, adding an agent with lipoxygenase 3 enzyme and allowing the plant parts to remain in contact with the enzyme under conditions effective to attain the desired effect. An improved composition comprises a hexanal producing plant part meal or …

Grass Control In Corn With Accent And Beacon, W. W. Witt, Charles H. Slack, James R. Martin, Jonathan D. Green, M. A. Thompson

Agronomy Notes

The traditional method for grass control in corn is based on using herbicides applied to soil and incorporated before planting or to the soil surface after planting. This approach has been successful in controlling crabgrass, fall panicum and foxtails, but johnsongrass and shattercane control was sometimes inadequate.

Shade Tree Decline And Related Problems, John Hartman, Mary L. Witt

Agriculture and Natural Resources Publications

Shade tree decline is a complex disease situation since the cause frequently cannot be traced to one single factor. Typically, one or more primary stresses cause the health of a tree to deteriorate, and then secondary pathogens and/or insects contribute to further decline. Determining causes of decline requires careful examination of the tree and growing site as well as knowledge of the tree's history. Even then, diagnosis may be difficult because the original stress may be obscure or no longer present. Trees most commonly affected in Kentucky are Norway and sugar maples, ash and oaks (particularly pin oaks).

Nitrogen Fertilizer Use By A High Yielding No-Till Corn Crop, Kenneth L. Wells, H. B. Rice, William O. Thom

Agronomy Notes

At the present time there is public concern about the effect which nitrogen (N) fertilizer use by farmers has on groundwater quality. This has resulted in research efforts to describe what happens to nitrogen fertilizer after it is applied to a crop. We have conducted a study for the past 3 years which enables us to make a reasonable estimate of how much fertilizer N is taken up by a high yielding corn crop. And, depending on whether the corn is harvested for silage or grain, we have estimated how much of the fertilizer N is removed from the field. …

Β-Ionone Derivatives As Antifungal Agent, Joseph Kuc, Steven D. Salt

Plant Pathology Faculty Patents

To read this abstract, please download this patent.

Method For X And Y Spermatozoa Separation, Panayiotis M. Zavos, Karl A. Dawson

Animal and Food Sciences Faculty Patents

A method for separating male and female determining spermatozoa includes the initial step of exposing freshly ejaculated spermatozoa in a substantially protein free diluent to an excess concentration of a monoclonal antibody directed against H-Y antigen that binds substantially exclusively with male determining spermatozoa. The method continues with the suspending of the exposed spermatozoa together with a conjugate of (1) an immunoglobulin G antibody that binds substantially exclusively to the monoclonal antibody and (2) an immunoabsorbant substrate in a substantially protein free diluent. This forms a conjugate/spermatozoa preparation. The method concludes with the recovering of the separated male and female …

1990 Kentucky Winter Annual Legume Variety Test, L. M. Lauriault, Norman L. Taylor, Jimmy C. Henning, W. T. Edmonson

Agronomy Notes

Cover crops are often used in Kentucky following the harvest of row crops such as tobacco, corn, and soybeans. Living cover crops can prevent erosion, reduce leaching of nutrients, and supply grazing, green manure, or a plant cover in which to no-till the following spring.

Curling Burley Tobacco From An Automated Harvesting System, Linus R. Walton, Larry G. Wells, James H. Casada

Biosystems and Agricultural Engineering Faculty Publications

If burley tobacco can be successfully cured at high density under waterproof covers in the field, a producer can expand production without the necessity of building new curing barns and can thereby more easily justify investment in the automated burley tobacco harvesting system (Wells et al., 1990a, b). Curing under waterproof covers in the field and curing on frames in the barn were evaluated over three curing seasons using two varieties (KY 14 and TN 86), two plant densities (32 and 43 plants/m², 3 and 4 plants/ft²), position of tobacco on the frame (four levels ranging …

Grazing Alfalfa, Harlan E. White

Kentucky Alfalfa and Stored Forage Conference

Alfalfa has tremendous potential as a pasture plant. The characteristics such as high yields, high quality, persistence, and drought tolerance that make it so valuable for hay and silage production also make it extremely useful for grazing. Properly managed so it has adequate recovery periods after being grazed, it can provide grazing over a six-month grazing season.

Hay Testing And Marketing Update, Jimmy C. Henning

Kentucky Alfalfa and Stored Forage Conference

The hay testing program of the Kentucky Department of Agriculture continues to be actively involved across the state. Presently, there are stationary NIR labs in Frankfort, Glasgow, and Paducah. These labs operate somewhat differently from traditional forage testing labs because, for the price of analyzing the forage sample ($10), they will actually come to the farm and sample your hay! To get your forage analyzed, call the Department of Agriculture in Frankfort at 1-800-248-4628. They will find a convenient time to meet with you at your bam for sampling. The KDA. samplers will also rate your hay for several visual …

The Role In Alfalfa In Soil Conservation, Glenn D. Johnson

Kentucky Alfalfa and Stored Forage Conference

Due to its many benefits, alfalfa has earned its reputation as "Queen of the Forages". Some of alfalfa's advantages are 1) very high nutritional value for all classes of livestock, 2) adaptability to a wide range of soils and growing conditions, 3) flexibility in being able to be grown alone or with grasses, 4) to be harvested for hay, silage or pasture, 5) strong seedling vigor and 6) high yields.

Alfalfa also should be rated "Queen of the Forages" as a crop for the soil. In addition to its many other benefits, alfalfa is an excellent choice for soil improvement …

Weed Control Strategies For Alfalfa Production, James R. Martin

Kentucky Alfalfa and Stored Forage Conference

The importance of weed control in alfalfa production should not be overlooked especially when you consider the high investment associated with this crop.

Management Options For Thinning Alfalfa Stands, Garry D. Lacefield

Kentucky Alfalfa and Stored Forage Conference

Alfalfa "Queen of the Forage Crops", is the most important forage legume grown in the United States. It is widely adapted and is grown over a wide range of soil and climatic conditions. Alfalfa has the highest yield potential and highest feeding value of all adapted perennial forage legumes. It is a versatile crop which can be used for pasture, hay, silage, green-chop, soil improvements and human consumption (sprouts, etc.). As a result of this crops many merits, especially yield, quality and versatile use, it can be used successfully in many animal feeding programs.

Alfalfa Varieties, Jimmy C. Henning

Kentucky Alfalfa and Stored Forage Conference

Few areas of forage agriculture are changing faster than variety development in alfalfa. The 1990 revised "Alfalfa Varieties" publication of the Certified Alfalfa Seed Council listed 26 new alfalfa varieties. Of the available certified alfalfa varieties, only 10 to 15% are public varieties. The remainder are developed and marketed by commercial companies such as Agripro, Dairy land, FFR, Pioneer, Vista, W-L Research, and others. The need for yield and performance information on alfalfa varieties therefore increases yearly. In 1990, the alfalfa variety testing program was re-started under the leadership of the University of Kentucky and Western Kentucky University (WKU). Three …

Procedures For No-Till Alfalfa, Harlan E. White

Kentucky Alfalfa and Stored Forage Conference

Technology is now available to successfully establish new stands of alfalfa without the need for tillage and preparation of a fine seedbed. Establishing new stands of alfalfa no-till essentially eliminates the threat of soil erosion while the new seeding is becoming established as well as the resulting ruts and gullies which damage equipment and can be dangerous to equipment operators. In addition to conserving soil, no-till seedings conserve moisture already present in the seedbed. This, plus the dramatic reduction in water run-off, improves the moisture supply for new seedlings. Less time and fuel are required to seed using no-till methods …

Foreword [1991], Garry D. Lacefield

Kentucky Alfalfa and Stored Forage Conference

This is the front matter of the proceedings.

1990 Kentucky Red Clover Variety Test, L. M. Lauriault, Norman L. Taylor, Jimmy C. Henning, W. T. Edmonson

Agronomy Notes

Red clover is a high quality, shortlived perennial legume that is used in mixed or pure stands for pasture, hay, silage, green chop, and soil improvement. This species is adapted to a wide range of climatic and soil conditions and therefore is very versatile as a forage crop. Stands are generally productive for two or three years with the highest yields occurring in the year following establishment. Red clover is used primarily as a renovation legume for grass pastures. It is the dominant legume in Kentucky because it is high in seedling vigor, quality, yield, and animal acceptance.

Physiology Of Murine B Lymphocytes Ii. Life-Spans Of Mitogen And Thymus-Independent Antigen (Type 2) Reactive B Lymphocytes From Aged Mice, Venkatachalam Udhayakumar, S. Niranjan Goud, Richard J. Cross, Subbarao Bondada

Microbiology, Immunology, and Molecular Genetics Faculty Publications

We showed earlier that life spans of murine B lymphocytes could be estimated by measuring the functional reactivities of normal B cells upon transfer into x-linked immunodeficient (xid) mice, which do not respond to anti-mouse IgM (anti-μ) antibodies and thymic-independent type -2 (TI-2) antigens. Here the same approach was adopted, to evaluate the life spans of B-lymphocytes from aged mice. Spleen cells from normal young and aged mice were transferred into young or aged xid recipients and the decay kinetics were followed by measuring the proliferative response to anti-μ and PFC response to TNP-Ficoll, a prototype TI-2 antigen. The results …

Elliptical Combing Motion For Harvesting Bell Peppers, J. H. Wilhoit, George A. Duncan, Larry G. Wells

Biosystems and Agricultural Engineering Faculty Publications

A new concept for mechanically harvesting bell peppers, intended specifically for multiple-pass harvesting, utilized spaced horizontal fingers combing vertically upward through plant foliage in an elliptical path alternating from both sides of the row. An apparatus based on this concept was designed and built, and harvest tests were conducted to determine the effect of two main machine operating parameters, vertical picking speed and disk angle. Harvesting performance was evaluated in terms of harvest efficiency, fruit damage, and plant damage as it related to multiple-pass harvesting. Fruit removal, both harvestable size and immature, increased significantly as vertical picking speed increased. Branch …

Triazine Resistant Pigweeds In Kentucky Corn Fields, Jonathan D. Green, Michael Barett, Mike Radford

Agronomy Notes

Smooth pigweed (Amaranthus hybridus) control from atrazine and/or simazine (Princep) has been unsatisfactory in some Kentucky corn fields. Many of these fields have grown corn where one or both of these herbicides have been used continuously for several years. These two triazine herbicides, particularly atrazine, are used annually on over 90% of the corn grown in Kentucky. Other areas in the U.S. and around the world have reported. unsatisfactory control from atrazine where triazine resistant pigweed has developed.

Automated Harvesting Of Burley Tobacco Ii. Evaluation Of System Performance, Larry G. Wells, George B. Day V, Timothy D. Smith

Biosystems and Agricultural Engineering Faculty Publications

A prototype system for fully automated harvesting of burley tobacco has been developed and tested. Three years of field testing has shown that mechanical losses associated with the system were only slightly higher than via conventional methods. The system performed reliably at a sustained harvesting rate of approximately 1.4 ha/day (3.4 acre/day), while indicating that a rate of 2 ha/day (5 acre/day) should be easily achievable. The system is operated by two workers and reduces conventional labor requirement by approximately 80-85%.

Automated Harvesting Of Burley Tobacco I. System Development, Larry G. Wells, George B. Day V, Timothy D. Smith

Biosystems and Agricultural Engineering Faculty Publications

A fully automated system for harvesting and handling mature buriey tobacco has been developed. This article identifies the operations essential to this harvesting concept and describes the development of the mechanisms by which they were accomplished. The system detaches, inverts and places mature plants into portable holders for air curing under waterproof covering without requiring any manual handling of the crop. Manual labor currently required to harvest buriey tobacco would be reduced by 80-85% and the system would eliminate the drudgery associated with manual handling. The harvesting system has an approximate capacity of 1.4 to 2.0 ha/day (3.5 to 5.0 …