Life Sciences Commons^™

Making Forages Work Down On The Farm, Russell C. Hackley

Forage Symposium at the Kentucky Cattlemen’s Convention

No abstract provided.

Making Forages Work Down On The Farm, Jason Sandefur

Forage Symposium at the Kentucky Cattlemen’s Convention

No abstract provided.

Making Forages Work Down On The Farm, Lowell J. Clifford

Forage Symposium at the Kentucky Cattlemen’s Convention

No abstract provided.

Kentucky's Forage Resources, Garry D. Lacefield

Forage Symposium at the Kentucky Cattlemen’s Convention

Kentucky is a FORAGE state with approximately one-half of the total farmland occupied by forage grasses and legumes. We are very fortunate that we can grow many different species successfully. We have the natural resources and climate to grow most cool-season and warm-season species. We are fortunate that we usually have a relatively long growing season. We are usually blessed with rain, especially in spring (2007 was a major exception) with our annual rainfall approximately fifty inches. We are also fortunate that we have some of the absolute best forage-livestock producers in the World.

Foreword [2008], Garry D. Lacefield, Christi L. Forsythe

Forage Symposium at the Kentucky Cattlemen’s Convention

No abstract provided.

Genome-Wide Analysis Of The Interaction Between The Endosymbiotic Bacterium Wolbachia And Its Drosophila Host, Zhiyong Xi, Laurent Gavotte, Yan Xie, Stephen L. Dobson

Entomology Faculty Publications

BACKGROUND: Intracellular Wolbachia bacteria are obligate, maternally-inherited, endosymbionts found frequently in insects and other invertebrates. The success of Wolbachia can be attributed in part to an ability to alter host reproduction via mechanisms including cytoplasmic incompatibility (CI), parthenogenesis, feminization and male killing. Despite substantial scientific effort, the molecular mechanisms underlying the Wolbachia/host interaction are unknown.

RESULTS: Here, an in vitro Wolbachia infection was generated in the Drosophila S2 cell line, and transcription profiles of infected and uninfected cells were compared by microarray. Differentially-expressed patterns related to reproduction, immune response and heat stress response are observed, including multiple genes that have …

Forage News [2008-01], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• Heart of America Grazing Conference to be Held in Missouri
• Forage Producers to be Featured at KCA
• Plan to Attend the AFGC/SRM Forage Conference in Louisville January 26-31
• 2007 Variety Test Reports
• Clearing Up the Confusion Between Eating Meat, Cancer, and Obesity
• Teff Looks Good in Pennsylvania Trials
• Kentucky Facts
• Effect of Hay Feeding Methods on Cow Performance, Hay Waste, and Wintering Cost
• Kentucky's Agricultural Economy
• Upcoming Events

Kentucky Water Resources Research Institute Annual Technical Report Fy 2007, Kentucky Water Resources Research Institute, University Of Kentucky

KWRRI Annual Technical Reports (USGS’s 104b Grant Program)

The 2007 Annual Technical Report for Kentucky consolidates reporting requirements of the Section 104(b) base grant award into a single document that includes: 1) a synopsis of each student research enhancement project conducted during the period, 2) citations for related publications, reports, and presentations, 3) a description of information transfer activities, 4) a summary of student support during the reporting period, and 5) notable awards and achievements during the year.

Optimizing Qpcr For The Quantification Of Periodontal Pathogens In A Complex Plaque Biofilm, Sreenatha S. Kirakodu, M. Govindaswami, Michael John Novak, Jeffrey L. Ebersole, Karen F. Novak

Center for Oral Health Research Faculty Publications

Quantitative PCR (qPCR) has recently been used to quantify microorganisms in complex communities, including dental plaque biofilms. However, there is variability in the qPCR protocols being used. This study was designed to evaluate the validity of two of these variables with the intent of developing a more standardized qPCR protocol. The two variables evaluated were (1) the use of DNA content versus actual cell counts to estimate bacterial numbers in mixed plaque samples and (2) the effectiveness of three different universal primers versus species specific primers in amplifying specific target pathogens in these samples. Results lead to the development of …

Packing Factors Of Feed Products In Storage Structures, Samuel G. Mcneill, Michael D. Montross, Sidney A. Thompson, I. Joseph Ross, Thomas C. Bridges

Biosystems and Agricultural Engineering Faculty Publications

Experiments were conducted to measure the changes in bulk density of cracked corn, corn meal, soybean meal, cotton seed meal, and distillers dried grain (without solubles) when subjected to simulated overburden pressures. All materials were tested at two moisture content levels (approximately 8% and 12% w.b.) and seven pressures between 0 and 69 kPa (0 and 10 psi). A mathematical model was fitted to the data to predict the bulk density of each feed ingredient as a function of pressure and moisture content. These relationships were inserted into a previously developed computer model to predict ingredient packing within conventional storage …

Airflow Resistance Of Wheat Bedding As Influenced By The Filling Method, J. Łukaszuk, M. Molenda, J. Horabik, B. Szot, Michael D. Montross

Biosystems and Agricultural Engineering Faculty Publications

A study was conducted to estimate the degree of variability of the airflow resistance in wheat caused by the filling method, compaction of the sample, and airflow direction. Two types of grain chambers were used: a cylindrical column 0.95 m high and 0.196 m in diameter, and a cubical box of 0.35 m side. All factors examined were found to influence considerably the airflow resistance. Gravitational axial filling of the grain column from three heights (0.0, 0.95 and 1.8 m) resulted in the pressure drops of 1.0, 1.3, and 1.5 kPa at the airflow velocity of 0.3 m/s. Consolidation of …

The Kentucky Agricultural Economic Outlook For 2008, Laura A. Powers

Kentucky Agricultural Economic Outlook

No abstract provided.

Caffeine Consumption Patterns And Beliefs Of College Freshmen, Gary Eugene Mcilvain

University of Kentucky Doctoral Dissertations

Recently, energy drinks that contain high caffeine content without any age restrictions for purchase, have been introduced into the U.S. market. Caffeine consumption in the U.S. has increased dramatically, resulting in an increase in emergency room visits and calls to poison control centers. This increase in energy drink consumption, along with the traditional coffees, teas, and soft drinks that people consume regularly, have pushed caffeine consumption to new highs in a multi-billion dollar market.

The purpose of this study was to determine (1) the amount of caffeine consumed by a sample of freshmen students at Marshall University, (2) their beliefs …

Microbial Community Structure Dynamics In Ohio River Sediments During Reductive Dechlorination Of Pcbs, Andres Enrique Nunez

University of Kentucky Doctoral Dissertations

The entire stretch of the Ohio River is under fish consumption advisories due to contamination with polychlorinated biphenyls (PCBs). In this study, natural attenuation and biostimulation of PCBs and microbial communities responsible for PCB transformations were investigated in Ohio River sediments.

Natural attenuation of PCBs was negligible in sediments, which was likely attributed to low temperature conditions during most of the year, as well as low amounts of available nitrogen, phosphorus, and organic carbon. Moreover, surface sediments were relatively oxidized, as indicated by the prevalence of aerobic bacteria such as beta- Proteobacteria, alpha-Proteobacteria, Sphingobacteria, and Nitrospira in 16S rRNA sediment …

Assessment Of Synaptic Function During Short-Term Facilitation In Motor Nerve Terminals In The Crayfish, Mohati Desai-Shah, Kert Viele, G. Sparks, J. Nadolski, B. Hayden, V. K. Srinivasan, Robin L. Cooper

Biology Faculty Publications

An enhanced buildup of [Ca²⁺]i occurs during short-term facilitation (STF) at the crayfish neuromuscular junction (NMJ). As a model system, this NMJ allows discrete postsynaptic quantal events to be counted and characterized in relation to STF. Providing 10 pulses, at 20 and 40Hz, we monitored postsynaptic quantal events over a discrete region of a nerve terminal with a focal macropatch electrode. Characteristics of quantal events were clustered into groups by peak amplitude and time to the peak amplitude. Since the synapses at this NMJ have varied spacing of active zones, number of active zones and synaptic size, the …

Presynaptic Depression In Phasic Motor Nerve Terminals And Influence Of 5-Ht On Vesicle Dynamics, A. F. M. Johnstone, S. S. Kellie, Robin L. Cooper

Biology Faculty Publications

Synaptic depression that is induced by electrical stimulation of the glutamatergic neuromuscular junction (NMJ) of the crayfish can be offset by recruitment of vesicles from a presynaptic reserve pool. This recruitment occurs following treatment of the NMJ with serotonin (5-HT), which results in a delay in the onset of synaptic depression induced by high frequency stimulation. The results of this study demonstrate that the releasable vesicles are insufficiently replenished during high frequency stimulation and that the readily releasable pool of vesicles (RRP) can be enhanced by the reserve pool (RP) in the presence of 5-HT. Anatomical visualization of vesicular pools …

Recent Advances In Medicago Truncatula Genomics, Jean-Michel Ané, Hongyan Zhu, Julia Frugoli

Plant and Soil Sciences Faculty Publications

Legume rotation has allowed a consistent increase in crop yield and consequently in human population since the antiquity. Legumes will also be instrumental in our ability to maintain the sustainability of our agriculture while facing the challenges of increasing food and biofuel demand. Medicago truncatula and Lotus japonicus have emerged during the last decade as two major model systems for legume biology. Initially developed to dissect plant-microbe symbiotic interactions and especially legume nodulation, these two models are now widely used in a variety of biological fields from plant physiology and development to population genetics and structural genomics. This review highlights …

Molecular, Genetic And Biochemical Characterization Of Oleic Acid- And Glycerol-Mediated Signaling In Plant Defense, Srivathsa C. Venugopal

Theses and Dissertations--Plant Pathology

Oleic acid (18:1) is one of the important monounsaturated fatty acids, which is synthesized upon desaturation of stearic acid and this reaction is catalyzed by the SSI2 encoded stearoyl-acyl-carrier-protein-desaturase. A mutation in SSI2 leads to constitutive activation of salicylic acid (SA)-mediated defense responses. Consequently, these plants accumulate high levels of SA and show enhanced resistance to bacterial and oomycete pathogens. Replenishing 18:1 levels in ssi2 plants, via a second site mutation in GLY1 encoded glycerol-3-phosphate (G3P) dehydrogenase, suppresses all the ssi2-triggered phenotypes. Study of mechanism(s) underlying gly1-mediated suppression of ssi2 phenotypes showed that 18:1 levels are regulated via …

Comparison Of Nutritional Intake Of Home School Children And Public School Children: A Comparison Study, Stephen D. Perry

University of Kentucky Master's Theses

Purpose: To compare selected food /nutrient consumption between families that educate their children at home with those that educate their children in the public school system.

Methods: The study sample included 112 children aged 7-11 years in Fayette Co Kentucky. The children were divided into groups according type of education. There were 65 home-schooled children and 47 children who attend public school. Subjects recorded their dietary intake for one week. The data were analyzed using Nutrition Data Software for Research (2006). Comparisons were made for the intake of selected nutrients using two tailed independent sample t-tests.

Results: The public school …

Parental Perceptions Of Nutritional Status Of Children With Autism, Rachel Corliss Holt

University of Kentucky Master's Theses

Literature shows that nutritional deficiency and feeding behaviors in neurodevelopmentally disabled children is secondary to an underdeveloped gastrointestinal tract. Fifty (50) parents with children with autism responded to a Nutritional Perception Assessment that they believe their children are at adequate nutritional status. They reported confidence in their nutritional knowledge, yet admitted to concern about the nutritional status of their children. The survey revealed that parents are not seeing a registered dietitian for their nutritional advice, leading to the question of soundness in nutritional information received. A marginal correlation was found between belief in adequate nutritional status of children and the …

Content Preparation Of Pre-Service Agricultural Education Teachers And Its Influence On Their Content Knowledge, Amber Houck

University of Kentucky Master's Theses

Content knowledge preparation for teachers is a crucial component of the modal curriculum model for education. The purpose of this study was to determine if the amount and quality of coursework preparation of pre-service University of Kentucky agriculture teachers influences their content knowledge as defined by the Praxis II agriculture exam scores. This study concluded that there was variability in coursework preparation of pre-service agriculture teachers at the University of Kentucky. Praxis II exam scores of pre-service teachers indicated that most students are meeting an adequate content knowledge level based on the exam material. It can also be concluded that …

The Metastasis Suppressor Nm23-H1 Is Required For Dna Repair, Mengmeng Yang

University of Kentucky Doctoral Dissertations

NM23-H1 represents the first identified metastasis suppressor, exhibiting reduced expression in breast carcinoma and melanoma, and an ability to inhibit metastatic growth without significant impact on the transformed phenotype. Although its molecular mechanism of action is not fully understood, NM23-H1 possesses at least three enzymatic activities that may mediate metastasis suppressor function. It catalyzes nucleoside diphosphate kinase (NDPK) activity, as well as protein histidine kinase and 3’-5’ exonuclease activities. As 3’-5’ exonucleases are generally required for maintenance of genomic integrity, this activity represents a plausible mediator to underlie the metastasis suppressor function of NM23-H1 protein. To investigate the relevant activity …

Colloid Mediated Transport Of Heavy Metals In Soils Following Reclamation With And Without Biosolid Application, Jarrod O. Miller

University of Kentucky Doctoral Dissertations

Soils disturbed by strip mining practices may have increased colloid loads moving to groundwater resources, also enhancing the transport of contaminants into our water resources. We hypothesize that contaminant transport within soils following mining is enhanced by colloid mobility. Two sites were chosen for this study, a 30-year old reclaimed strip mine in southwest Virginia and a recently mined area from eastern Kentucky. Intact reclaimed soil monoliths were retrieved from sandstone derived soils in southwestern Virginia. Reclaimed monoliths from eastern Kentucky were recreated in the lab. Intact undisturbed (native) soil monoliths representing the soils before mining were also sampled for …

Functional Characterization Of Upd3 In Drosophila Development, Liqun Wang

University of Kentucky Doctoral Dissertations

The JAK/STAT pathway is a non-receptor tyrosine kinase signaling pathway that is well conserved and highly re-utilized in many mammalian and Drosophila developmental processes. Compared to dozens of ligands and receptors in mammalian JAK/STAT, Drosophila JAK/STAT pathway is simpler with one receptor and three ligands, Upd, Upd2 and Upd3, which have similar amino acid sequences. Previous literature shows that upd and upd2 exhibit the same dynamic striped expression pattern in embryos and have semi-redundant functions during embryogenesis. Do Upd and Upd3 also have redundant functions? To answer this question, the functions of Upd3 in Drosophila development were investigated in this …

Map-Based Cloning Of An Anthracnose Resistance Gene In Medicago Truncatula, Shengming Yang

University of Kentucky Doctoral Dissertations

Anthracnose, caused by the fungal pathogen Colletotrichum trifolii, is one of the most destructive diseases of alfalfa worldwide. Cloning and characterization of the host resistance (R) genes against the pathogen will improve our knowledge of molecular mechanisms underlying host resistance and facilitate the development of resistant alfalfa cultivars. However, the intractable genetic system of cultivated alfalfa, owing to its tetrasomic inheritance and outcrossing nature, limits the ability to carry out genetic analysis in alfalfa. Nonetheless, the model legume Medicago truncatula, a close relative of alfalfa, provides a surrogate for cloning the counterparts of many agronomically important genes in …

Comparative Biology Of Seed Dormancy-Break And Germination In Convolvulaceae (Asterids, Solanales), Kariyawasam Marthinna Gamage Gehan Jayasuriya

University of Kentucky Doctoral Dissertations

The biology of seed dormancy and germination of 46 species representing 11 of the 12 tribes in Convolvulaceae were compared in laboratory (mostly), field and greenhouse experiments. Seeds were tested for kind of dormancy and storage behavior; artificial or simulated natural treatments were applied to break physical dormancy (PY); the initial route of water entry (“water gap”) into seeds was identified; the morphoanatomy of the water gap was compared in seeds of 17 species; ontogenetical differences between water gap and seed coat away from the hilum were described in Ipomoea lacunosa seeds; cycling of sensitivity to dormancy break was elucidated …

Roles Of Micrornas In Plant Abiotic Stress, Development And Viral Infection, Venugopal Mendu

University of Kentucky Doctoral Dissertations

Plant microRNAs play important roles in plant growth and development. Here we investigated the roles of miRNAs in the plant abiotic stress, development and viral infection. MicroRNA membrane array analysis using five different abiotic stress treatments resulted in the identification of 8 novel stress inducible miRNA-families. Functional studies on novel stress inducible miR168 revealed its functional relation with abiotic stress. Over expression of miR168 in Arabidopsis showed upregulation of four stress related miRNAs (miR163, miR167, miR398 and miR408). Analysis of 9 independent transgenic lines showed induction of miR398, an oxidative stress responsive miRNA with a corresponding down regulation of its …

The Influence Of Ca2+ Regulation In Synaptic Facilitation Of Motor Nerve Terminals In Crayfish And Drosophila As Well As In The Physiological Regulation Of Larval Drosophila Heart, Mohati Desai-Shah

University of Kentucky Doctoral Dissertations

Intracellular Ca²⁺ ions are highly regulated in animal cells for them to function normally. Since the tight regulation of [Ca²⁺]i is so ubiquitous among cells, it is not surprising that altered function in [Ca²⁺]i regulation is associated with a myriad of disease states in humans. This is particularly evident in pacing myocytes and nerve terminals related to synaptic transmission. A common thread through this dissertation is on the role of three regulators proteins that are common to many cell types. These are the plasmalemmal Na⁺/Ca²⁺ exchanger (NCX), the Ca²⁺-ATPase (PMCA) …

The Role Of Spiders In The Detrital Food Web Of An Eastern Deciduous Forest, Erin Elizabeth Hladilek

University of Kentucky Doctoral Dissertations

Historically, terrestrial food web research has focused on describing the structure of aboveground grazing webs, and determining how interactions among plants, herbivores and higher trophic levels influence primary productivity. Detrital food webs however, play a significant role in regulation of ecosystem dynamics through direct impacts on decomposition. Unraveling the complex nature of detrital food web structure is critical to developing a better understanding of ecosystem function. Therefore the primary objective of this research was to describe the structure of the leaf-litter food web in a temperate deciduous forest, with emphasis on interactions between a community of generalist predators, the forest-floor …

Assessment Of Terrain Attribute Models For The Identification Of Erosion Prone Areas Suitable For The Establishment Of Grassed Waterways In An Agricultural Field Setting In The Out Bluegrass Region Of Kentucky, Adam Clellon Pike

University of Kentucky Master's Theses

The speed and accuracy of conservation planning could be improved if maps indicating areas where grassed waterways should be placed to reduce erosion could be easily created. For five central Kentucky fields, elevation data were obtained with real time kinematic (RTK) global positioning system (GPS) and from US Geological Survey (USGS) digital elevation models (DEMs). Terrain attributes were calculated from these datasets which were used as predictor variables for neural network and logistic regression analyses. Grassed waterway prediction models were developed with these analyses. The type of activation function, type of standardization procedure, number of neurons, number of preliminary runs, …