Life Sciences Commons^™

Poster Session, Kentucky Water Resources Research Institute, University Of Kentucky

Kentucky Water Resources Annual Symposium

• Kentucky Water Awareness Month Packet, Ashley Osborne, UK Cooperative Extension Service, ENRI Task Force
• Recent Enhancements to the Kentucky Groundwater-Quality Data Search Engine, Bart Davidson and R. Stephen Fisher, KGS
• Diatom Colonization Patterns and Carbon Stable Isotopic Ratios in Developing Periphyton at Springs of Differing Geologic Origin in Land-Between-the-Lakes, Courtney Snapp and Susan Hendricks, Water Sciences Program, Murray State University
• Seasonal Changes in Stratification and Oxygen Content of a Eutrophic Lake, Wilgreen Lake, Madison County, Kentucky, Jill Hunter and Walter Borowski, Dept of Geography and Geology, EKU
• Water Pioneers Water Quality Program 2007, Stephanie Jenkins, …

Session 2d: Pathogen Transport, Kentucky Water Resources Research Institute, University Of Kentucky

Kentucky Water Resources Annual Symposium

• Assessment of Nonpoint Source Impacts on Groundwater Quality in South Elkhorn Creek Basin, Central Kentucky, Robert J. Blair and others, Kentucky Division of Water, Frankfort
• Karst Groundwater Infiltration of the Sanitary Sewer within the Beargrass Creek Watershed, Jefferson County, Kentucky, Joseph Ray and others, Kentucky Division of Water
• Illicit Discharge into Town Branch in Lexington, Kentucky, Jim Buckles and others, Tetra Tech, Lexington
• Assessment of Water Quality Trends in the Upper Forks of the Kentucky River Basin: Focus on Pathogen Impairment, Chandramouli Viswanathan, Kentucky Water Resources Research Institute, UK

Session 2c: Water Quality, Kentucky Water Resources Research Institute, University Of Kentucky

Kentucky Water Resources Annual Symposium

• Determinations of Bioavailable Fractions in the Assessment of Metals in Big and Little Bayou Creeks……, McCracken County, Kentucky, David J. Price, Dept Biology, UK
• Creation of a Catalog of Environmental Mercury Databases in Kentucky, Caroline Chan and others, School of Public Health and Information Sciences, U of L
• Using Nitrogen Isotopes to Trace Nutrient Inputs to a Eutrophic Lake, Wilgreen Lake, Madison County, Kentucky, Walter Borowski and Theresa Aguiar, Dept of Geography and Geology, EKU
• Using Microbial Distribution and Abundance in a Eutrophic Lake as a Tracer for Nutrient Inputs, Wilgreen Lake, Madison County Kentucky, Theresa …

Session 2b: Variety, Kentucky Water Resources Research Institute, University Of Kentucky

Kentucky Water Resources Annual Symposium

• Mud, Horses and Clean Water – A BMP Demonstration Project for Suburban Horse Owners, Steve Higgins and others, Biosystems and Ag Engineering, UK
• A Topological Model of Open Channel Flows, Brian Belcher and Jimmy Fox, Dept of Civil Engineering, UK
• Rain Gardens: Restoring a Waste Water to a Priceless Treasure, H. David Gabbard, LFUCG, Richard Walker, Tetra Tech, and Sandy Camargo, CDP Engineers
• Kentucky Erosion Prevention and Sediment Control (KESPSC) – An Answer to the Question “What is a Qualified Inspector?” Shelby Jett, Kentucky Transportation Cabinet, Kevin Gibson and John Ricketts, Stantec Consulting Services, Lexington

Session 2a: Groundwater, Kentucky Water Resources Research Institute, University Of Kentucky

Kentucky Water Resources Annual Symposium

• How Close Do We Need to Sample for Appropriate Solute Transport Characterization Through the Vadose Zone? Ole Wendroth and others, Dept of Plant and Soil Sciences, UK
• Efficiently Locating and Repairing Damaged Sewer Lines in a Karst Terrane, Kevin Lyne, LFUCG and Robert Blair, KDOW
• Hydrogeologic Investigations of Pavement Subsidence in the Cumberland Gap Tunnel, Jim Dinger and others, KGS

Session 1d: Land Use, Kentucky Water Resources Research Institute, University Of Kentucky

Kentucky Water Resources Annual Symposium

• Mountaintop Mining and Sustainable Development Opportunities for Appalachia, J. Stephen Gardner, Engineering Consulting Services, Inc., Lexington
• Spatial Characterization of Kentucky’s 2005-2001 Land Cover Change, A Kentucky Landscape Census Product, Demetrio Zourarakis and others, Kentucky Division of Geographic Information
• Feasibility Study of Cover-Collapse Rate in the Western Pennyroyal Karst of Kentucky, James Currens and Randall Paylor, KGS
• Kentucky SPARROW Model and its Applications to Understanding Nutrient Loads, Tanja N. Williamson, USGS Kentucky Water Science Center

Session 1c: Student Research Projects, Kentucky Water Resources Research Institute, University Of Kentucky

Kentucky Water Resources Annual Symposium

• A 3-D Computational Fluid Dynamics Code for Simulation of Pump Stations with Vertical Plunging Jet and Air Bubble Transport, Tien Mun Yee and Scott Yost, Dept of Civil Engineering, UK
• Chemical Evolution of Groundwater in the Wilcox Aquifer of the Mississippi Embayment, Estifanos Haile and Alan Fryar, Dept of Earth and Environmental Sciences, UK
• Sediment Organic Matter Degradation within Storage Zones Deposited In-Stream, Charles Davis and Jimmy Fox, Dept of Civil Engineering, UK
• Effectiveness of Improved Skid Trail Headwater Stream Crossings, Jeffrey Stringer and Christopher Reeves, Dept of Forestry, UK

Session 1b: Education, Kentucky Water Resources Research Institute, University Of Kentucky

Kentucky Water Resources Annual Symposium

• Community Based Science, Water Quality and Nonpoint Source Pollution, Carol Hanley and others, Tracy Farmer Center for the Environment, UK
• A Stormwater Education Program to Assist Kentucky’s MS4 Phase II Communities, Yvonne Meichtry and Julie Gee, Kentucky Transportation Cabinet, Frankfort
• Water/Wastewater Technician Training Institute: A Holistic Approach to Developing a Professional Water and Wastewater Workforce, Andrew Ernest, Center for Water Resource Studies, WKU
• Geology for Everyone, New Map Products from the Kentucky Geological Survey, Daniel Carey, KGS

Session 1a: Watersheds, Kentucky Water Resources Research Institute, University Of Kentucky

Kentucky Water Resources Annual Symposium

• Cane Run Watershed Assessment and Restoration Project, Steve Higgins and others, Biosystems and Ag Engineering, UK
• Kentucky Institute for Watershed Management Support, Andrew Ernest, Center for Water Resource Studies, WKU
• Watershed Planning Guidebook for Kentucky Communities, Katie Holmes and others, Kentucky Waterways Alliance

Proceedings Of 2008 Kentucky Water Resources Annual Symposium, Kentucky Water Resources Research Institute, University Of Kentucky

Kentucky Water Resources Annual Symposium

This conference was planned and conducted as part of the state water resources research annual program with the support and collaboration of the Department of the Interior, U.S. Geological Survey and the University of Kentucky Research Foundation, under Grant Agreement Number 06HQGR0087.

The views and conclusions contained in this document and presented at the symposium are those of the abstract authors and presenters and should not be interpreted as necessarily representing the official policies, either expressed or implied, of the U.S. Government or other symposium organizers and sponsors.

Forage News [2008-03], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• 28th Alfalfa Conference: Success in Spite of Worst Ice Storm in Our 28 Years
• Alfalfa Awards
• Alfalfa Hay Contest Awards
• 2007 Hay Summary
• Value and Amount of Nitrogen Fixed by Various Legumes
• Kentucky Farm Numbers and Livestock Operations
• The Differences Among Hay Conditioners
• AFGC Issues Resolution on Forage Crop Production
• Symposium to Examine Energy Potential of Forage Crops
• Upcoming Events

Enkephalin-Encoding Herpes Simplex Virus-1 Decreases Inflammation And Hotplate Sensitivity In A Chronic Pancreatitis Model, Hong Yang, Terry A. Mcnearney, Rong Chu, Ying Lu, Yong Ren, David C. Yeomans, Steven P. Wilson, Karin N. Westlund

Physiology Faculty Publications

Background: A chronic pancreatitis model was developed in young male Lewis rats fed a high-fat and alcohol liquid diet beginning at three weeks. The model was used to assess time course and efficacy of a replication defective herpes simplex virus type 1 vector construct delivering human cDNA encoding preproenkephalin (HSV-ENK).

Results: Most surprising was the relative lack of inflammation and tissue disruption after HSV-ENK treatment compared to the histopathology consistent with pancreatitis (inflammatory cell infiltration, edema, acinar cell hypertrophy, fibrosis) present as a result of the high-fat and alcohol diet in controls. The HSV-ENK vector delivered to the pancreatic surface …

The Α1d-Adrenergic Receptor Is Expressed Intracellularly And Coupled To Increases In Intracellular Calcium And Reactive Oxygen Species In Human Aortic Smooth Muscle Cells, Mary L. García-Cazarín, Jennifer L. Smith, Kyle A. Olszewski, Dan F. Mccune, Linda A. Simmerman, Robert W. Hadley, Susan D. Kraner, Michael T. Piascik

Pharmacology and Nutritional Sciences Faculty Publications

Background: The cellular localization of the α_1D-adrenergic receptor (α_1D-AR) is controversial. Studies in heterologous cell systems have shown that this receptor is expressed in intracellular compartments. Other studies show that dimerization with other ARs promotes the cell surface expression of the α_1D-AR. To assess the cellular localization in vascular smooth muscle cells, we developed an adenoviral vector for the efficient expression of a GFP labeled α_1D-AR. We also measured cellular localization with immunocytochemistry. Intracellular calcium levels, measurement of reactive oxygen species and contraction of the rat aorta were used as measures of …

Photo Highlights Of The 28th Kentucky Alfalfa Conference, Kentucky Alfalfa Conference

Kentucky Alfalfa and Stored Forage Conference

No abstract provided.

Hay Supply, Price And The Future, Tom Keene

Kentucky Alfalfa and Stored Forage Conference

As we prepare for the 2008 hay crop, it’s probably a good time to look back and see what the ’07 crop brought to us and wonder if it will have any bearing on the upcoming 2008 crop.

Alfalfa Hay And Balage: Testing For Quality, Kimberly Field

Kentucky Alfalfa and Stored Forage Conference

Why Test Your Hay?

• By knowing the percentages and protein levels you can increase/decrease the amount of supplements needed from your analysis report and will help the producer set feeding rations
• If you do not know what you have in your forage you are over or under feeding your livestock – therefore affecting the health of your animal and your pocket book

Is There A Benefit To Alfalfa Balage?, Gary Bates

Kentucky Alfalfa and Stored Forage Conference

Making hay in the mid-South has always been a difficult process. High humidity and rainfall often make drying a long, tedious, if not impossible proposition. Over the last few years there has been an increased interest in making round bale silage, or balage, from forage crops. Fermenting alfalfa for storage has several advantages and disadvantages over regular haymaking systems.

Alfalfa: Forage Crop Of The Future, Neal P. Martin, David Mertens, Ronald Hatfield, Hans Jung

Kentucky Alfalfa and Stored Forage Conference

In 2007, U.S. farmers harvested 23.6 million acres of alfalfa. Alfalfa harvested as hay and haylage produced 82.8 million tons valued at approximately $9.4 billion, ranking behind only corn and soybeans. Alfalfa hay supports dairy, beef, sheep, and horse production in the U.S. as well as a growing export market.

National Hay Association President's Perspective: What A Year To Be President, Ron Tombaugh

Kentucky Alfalfa and Stored Forage Conference

Over the years, I have had the opportunity to attend the Kentucky Alfalfa Conference in Cave City. Usually it was combined with a trip to deliver hay into Kentucky to either a dairy, beef, or horse customer. This is an excellent event.

The following is my “acceptance” speech given in September 2007 in Columbus, Ohio, when I was installed as President of the National Hay Association.

Certified Weed Free Hay And Straw Program, Kenny E. Perry

Kentucky Alfalfa and Stored Forage Conference

There is a growing demand in North America for the use of certified weed free hay and straw as a preventative program to limit the spread of noxious weeds. This voluntary certification program is designed to assure that hay and straw sold with proper certification identification meets minimum standards designed to limit the spread of noxious and undesirable weeds. Buyers are provided assurance that hay and straw certified through this program meets these minimum standards.

Alfalfa: Back To Basics, S. Ray Smith, Thomas Adam Probst

Kentucky Alfalfa and Stored Forage Conference

In the past, many university research trials and on-farm demonstrations have determined a range of cutting intervals for alfalfa. Most researchers have agreed that the optimum harvest frequency is about every 35 days to ensure top yields, without sacrificing too much in the way of forage quality and stand persistence. Others use a strategy of harvesting at 10% bloom.

Haymaking: A Trip Down Memory Lane, Garry D. Lacefield, John E. Baylor

Kentucky Alfalfa and Stored Forage Conference

On a hot day in August, I climbed into the dust-free air-conditioned cab of a new tractor to assist in a hay baling demonstration during a field day. Hay yield was low as a result of the drought, so it was important that I get everything from the small windrow. I adjusted the volume on the surround sound stereo where I had just inserted a CD of the Eagles Greatest Hits. Now we’re ready to bale – first let me check the GPS and get a check on the moisture of the hay coming into the bale chamber – moisture …

Foreword And Recipients Of Kentucky Alfalfa Awards [2008], Garry D. Lacefield, Christi L. Forsythe

Kentucky Alfalfa and Stored Forage Conference

No abstract provided.

Recycling Of Dolichyl Monophosphate To The Cytoplasmic Leaflet Of The Endoplasmic Reticulum After The Cleavage Of Dolichyl Pyrophosphate On The Lumenal Monolayer., Jeffrey S Rush, Ningguo Gao, Mark A Lehrman, Charles J Waechter

Molecular and Cellular Biochemistry Faculty Publications

During protein N-glycosylation, dolichyl pyrophosphate (Dol-P-P) is discharged in the lumenal monolayer of the endoplasmic reticulum (ER). Dol-P-P is then cleaved to Dol-P by Dol-P-P phosphatase (DPPase). Studies with the yeast mutant cwh8Delta, lacking DPPase activity, indicate that recycling of Dol-P produced by DPPase contributes significantly to the pool of Dol-P utilized for lipid intermediate biosynthesis on the cytoplasmic leaflet. Whether Dol-P formed in the lumen diffuses directly back to the cytoplasmic leaflet or is first dephosphorylated to dolichol has not been determined. Incubation of sealed ER vesicles from calf brain with acetyl-Asn-Tyr-Thr-NH(2), an N-glycosylatable peptide, to generate Dol-P-P in …

Effect Of Thyroid Hormone Concentration On The Transcriptional Response Underlying Induced Metamorphosis In The Mexican Axolotl (Ambystoma), Robert B. Page, Stephen R. Voss, Amy K. Samuels, Jeramiah J. Smith, Srikrishna Putta, Christopher K. Beachy

Biology Faculty Publications

BACKGROUND: Thyroid hormones (TH) induce gene expression programs that orchestrate amphibian metamorphosis. In contrast to anurans, many salamanders do not undergo metamorphosis in nature. However, they can be induced to undergo metamorphosis via exposure to thyroxine (T4). We induced metamorphosis in juvenile Mexican axolotls (Ambystoma mexicanum) using 5 and 50 nM T4, collected epidermal tissue from the head at four time points (Days 0, 2, 12, 28), and used microarray analysis to quantify mRNA abundances.

RESULTS: Individuals reared in the higher T4 concentration initiated morphological and transcriptional changes earlier and completed metamorphosis by Day 28. In contrast, initiation of metamorphosis …

Forage News [2008-02], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• Kentucky Alfalfa Conference: 28 Years and Going Strong
• Forages at KCA
• AFGC/SRM Conference a Huge Success
• KY Producer and Students Win National Forage Awards
• Heart of America Grazing Conference
• Roundup Ready Alfalfa Update
• Can You Establish Clover and Suppress Winter Annual Weeds
• AVAIL Polymer Applied to Phosphorous Fertilizer
• Restricting Intake of Forages: An Alternative Feeding Strategy for Wintering Beef Cows
• Restricting Time of Access to Large Round Bales of Hay Affects Hay Waste and Cow Performance
• Upcoming Events

The Expression Of Microrna Mir-107 Decreases Early In Alzheimer's Disease And May Accelerate Disease Progression Through Regulation Of Β-Site Amyloid Precursor Protein-Cleaving Enzyme 1, Wang-Xia Wang, Bernard W. Rajeev, Arnold J. Stromberg, Na Ren, Guiliang Tang, Qingwei Huang, Isidore Rigoutsos, Peter T. Nelson

Sanders-Brown Center on Aging Faculty Publications

MicroRNAs (miRNAs) are small regulatory RNAs that participate in posttranscriptional gene regulation in a sequence-specific manner. However, little is understood about the role(s) of miRNAs in Alzheimer's disease (AD). We used miRNA expression microarrays on RNA extracted from human brain tissue from the University of Kentucky Alzheimer's Disease Center Brain Bank with near-optimal clinicopathological correlation. Cases were separated into four groups: elderly nondemented with negligible AD-type pathology, nondemented with incipient AD pathology, mild cognitive impairment (MCI) with moderate AD pathology, and AD. Among the AD-related miRNA expression changes, miR-107 was exceptional because miR-107 levels decreased significantly even in patients with …

Pioglitazone Inhibition Of Lipopolysaccharide-Induced Nitric Oxide Synthase Is Associated With Altered Activity Of P38 Map Kinase And Pi3k/Akt, Bin Xing, Tao Xin, Randy Lee Hunter, Guoying Bing

Neuroscience Faculty Publications

BACKGROUND: Previous studies have suggested that peroxisome proliferator activated receptor-gamma (PPAR-gamma)-mediated neuroprotection involves inhibition of microglial activation and decreased expression and activity of inducible nitric oxide synthase (iNOS); however, the underlying molecular mechanisms have not yet been well established. In the present study we explored: (1) the effect of the PPAR-gamma agonist pioglitazone on lipopolysaccharide (LPS)-induced iNOS activity and nitric oxide (NO) generation by microglia; (2) the differential role of p38 mitogen-activated protein kinase (p38 MAPK), c-Jun NH(2)-terminal kinase (JNK), and phosphoinositide 3-kinase (PI3K) on LPS-induced NO generation; and (3) the regulation of p38 MAPK, JNK, and PI3K by pioglitazone. …

Interspecific Hybridization Yields Strategy For South Pacific Filariasis Vector Elimination, Corey L. Brelsfoard, Yves Séchan, Stephen L. Dobson

Entomology Faculty Publications

BACKGROUND: Lymphatic filariasis (LF) is a leading cause of disability in South Pacific regions, where >96% of the 1.7 million population are at risk of LF infection. As part of current global campaign, mass drug administration (MDA) has effectively reduced lymphatic filiariasis prevalence, but mosquito vector biology can complicate the MDA strategy. In some regions, there is evidence that the goal of LF elimination cannot be attained via MDA alone. Obligate vector mosquitoes provide additional targets for breaking the LF transmission cycle, but existing methods are ineffective for controlling the primary vector throughout much of the South Pacific, Aedes polynesiensis. …

Making Forages Work Down On The Farm, Todd Clark

Forage Symposium at the Kentucky Cattlemen’s Convention

No abstract provided.