Life Sciences Commons^™

Heavy Metal Accumulation In Seagrasses In Southeastern Florida, Erin Smith

HCNSO Student Theses and Dissertations

Seagrass beds are among the most ecologically important systems in the marine environment. They provide the primary production to nearby coral reef and mangrove communities, and seagrasses comprise a large component of the diets of many marine organisms including fishes, small invertebrate species, and many protected species such as manatees and sea turtles. This consumption provides a pathway for many contaminants to enter the marine food web via the seagrasses. The coastal location of seagrass beds causes them to be especially susceptible to anthropogenic pollution, including accumulation of heavy metals, which has been shown to have many adverse health effects …

A Novel On‐Chip Method For Differential Extraction Of Sperm In Forensic Cases, Fatih Inci, Mehmet O. Ozen, Yeseren Saylan, Morteza Miansari, Duygu Cimen, Raghu Dhara, Thiruppathiraja Chinnasamy, Mehmet Yuksekkaya, Chiara Filippini, Deepan Kishore Kumar, Semih Calamak, Yusuf Yesil, Naside Gozde Durmus, George Duncan, Leonard Klevan, Utkan Demirci

Biology Faculty Articles

One out of every six American women has been the victim of a sexual assault in their lifetime. However, the DNA casework backlog continues to increase outpacing the nation's capacity since DNA evidence processing in sexual assault casework remains a bottleneck due to laborious and time‐consuming differential extraction of victim's and perpetrator's cells. Additionally, a significant amount (60–90%) of male DNA evidence may be lost with existing procedures. Here, a microfluidic method is developed that selectively captures sperm using a unique oligosaccharide sequence (Sialyl‐LewisX), a major carbohydrate ligand for sperm‐egg binding. This method is validated with forensic mock samples dating …

Assessment Of Mesophotic Coral Ecosystem Connectivity For Proposed Expansion Of A Marine Sanctuary In The Northwest Gulf Of Mexico: Larval Dynamics, Lysel Garavelli, Michael S. Studivan, Joshua D. Voss, Alyson Kuba, Joana Figueiredo, Laurent M. Cherubin

Marine & Environmental Sciences Faculty Articles

In coral reef ecosystems, mesophotic coral habitat (>30 m to the end of the photic zone) are extensions of shallow reefs and contribute to the persistence of coral reef populations. In the North West Gulf of Mexico (NW GOM), the Flower Garden Banks National Marine Sanctuary (FGBNMS) is an isolated reef ecosystem comprising contiguous shallow and mesophotic reefs habitats on two central banks along the margin of the continental shelf. A future expansion of the sanctuary is proposed to include additional mesophotic banks and aims at building a network of protected areas in the NW GOM to ensure the …

A Temporal Analysis Of A Deep-Pelagic Crustacean Assemblage (Decapoda: Caridea: Oplophoridae And Pandalidae) In The Gulf Of Mexico After The Deepwater Horizon Oil Spill, Devan Nichols

HCNSO Student Theses and Dissertations

In 2010, the largest oil spill in U.S history occurred off the coast of Louisiana from April 20^th to September 19^th, when the well was declared officially sealed by the U.S Coast Guard, after releasing more than 4.4 million barrels of crude oil into the Gulf of Mexico (GOM) (McNutt et al., 2012). This spill was unique because it occurred in deep water approximately 1500 m below the ocean surface. Virtually nothing is known about the effects of oil spills on marine life in the deep sea, and there are limited data on mesopelagic and bathypelagic animals …

The Complete Phylogeny Of Pangolins: Scaling Up Resources For The Molecular Tracing Of The Most Trafficked Mammals On Earth, Philippe Gaubert, Agostinho Antunes, Hao Meng, Lin Miao, Stéphane Peigné, Fabienne Justy, Flobert Njiokou, Sylvain Dufour, Emmanuel Danquah, Jayanthi Alahakoon, Erik Verheyen, William T. Stanley, Stephen J. O'Brien, Warren E. Johnson, Shu Jin Luo

Biology Faculty Articles

Pangolins, considered the most-traffcked mammals on Earth, are rapidly heading to extinction. Eight extant species of these African and Asian scale-bodied anteaters are commonly recognized, but their evolutionary relationships remain largely unexplored. Here, we present the most comprehensive phylogenetic assessment of pangolins, based on genetic variation of complete mitogenomes and 9 nuclear genes. We confrm deep divergence among Asian and African pangolins occurring not later than the Oligocene-Miocene boundary ca. 23 million years ago (Ma) (95% HPD = 18.7-27.2), limited fossil evidence suggesting dispersals from Europe. We recognize 3 genera including Manis (Asian pangolins), Smutsia (large African pangolins), and Phataginus …

Fine Grained Compositional Analysis Of Port Everglades Inlet Microbiome Using High Throughput Dna Sequencing, Lauren M. O'Connell, Song Gao, Donald S. Mccorquodale Jr., Jay M. Fleisher, Jose Lopez

Marine & Environmental Sciences Faculty Articles

Background

Similar to natural rivers, manmade inlets connect inland runoff to the ocean. Port Everglades Inlet (PEI) is a busy cargo and cruise ship port in South Florida, which can act as a source of pollution to surrounding beaches and offshore coral reefs. Understanding the composition and fluctuations of bacterioplankton communities (“microbiomes”) in major port inlets is important due to potential impacts on surrounding environments. We hypothesize seasonal microbial fluctuations, which were profiled by high throughput 16S rRNA amplicon sequencing and analysis.

Methods & Results

Surface water samples were collected every week for one year. A total of four samples …

Ecological Correlates Of Community Structure In Seagrass-Associated Fishes In North Biscayne Bay And Port Of Miami, Florida, Elizabeth F. Colhoun

HCNSO Student Theses and Dissertations

Seagrass habitats are critical habitat for many fish species and are currently threatened by anthropogenic and natural factors, such as coastal development, pollution, global climate change, and sea level rise. There are few studies that have tracked long- term changes in seagrass habitat and their associated fish communities. This project addressed this need using data collected by the United States Geological Survey (USGS) from two South Florida sites, North Biscayne Bay, FL (NBB) and Port of Miami, FL (POM). The USGS sampling was part of ongoing monitoring projects designed to assist future management decisions that would enhance the protection of …

Assessment Of Metals In Tissues Of Marine-Associated Birds In South Florida, Caitlyn A. Nay

HCNSO Student Theses and Dissertations

Seabirds – broadly defined as any bird species associated with the marine environment – are exposed to a wide range of environmental contaminants. Vectors of exposure to metal pollution include by external contact, inhalation, and most often ingestion of food and incidental seawater. Seabirds are often considered marine ecosystem bioindicators due to their high trophic position, relatively long lifespan, and wide geographic ranges. We examined the concentration of total mercury (THg), lead (Pb), and cadmium (Cd), in the kidney, liver, breast muscle, and feathers of seven species of juvenile and adult seabirds commonly found in South Florida: brown pelican Pelecanus …

Evaluating The Efficacy Of Using Geomorphology As A Surrogate To Benthic Habitat On The Miami Terrace, Kim D. Smith

HCNSO Student Theses and Dissertations

The deep-sea is a vast and relatively sparsely characterized domain. As little as 0.01% of deep-sea benthic habitats have been characterized in detail. Characterizing the distribution of organisms and environmental components of the deep-sea is pivotal to the creation and implementation of successful resource management. Benthic habitat maps are a good method to inventory and characterize deep-sea habitats. Recent advances in technology, such as multibeam sonar and remotely operated vehicles (ROVs), have allowed for greater understanding of these ecosystems. As it is difficult and expensive to collect data deep-sea benthic community composition, environmental surrogates of biological data would be economically …

Patterns In Caribbean Coral Spawning, Anna C. Jordan

HCNSO Student Theses and Dissertations

Most corals worldwide are broadcast spawners that rely on synchronous gamete release for successful fertilization. Spawning synchrony may also decrease the probability of heterospecific fertilization that may produce maladaptive hybrids. Despite the importance of reproductive timing, researchers have only recently begun to collect spawning data across coral species in the Caribbean, but these data remain to be analyzed. This study investigates interannual, seasonal, and environmental patterns that may influence Caribbean scleractinian spawning times. The number of spawning observations varies widely among location and species. Most spawning observations were collected in Florida, Curaçao, and Flower Garden Banks National Marine Sanctuary. Acropora …

Population Dynamics Of The Threatened Staghorn Coral, Acropora Cervicornis, And The Development Of A Species-Specific Monitoring Protocol, Elizabeth Goergen

HCNSO Student Theses and Dissertations

Historically, Acropora cervicornis was found in high densities on many Caribbean, Florida, and Gulf of Mexico reefs. A disease outbreak in the late 1970s and 80s caused up to 99% loss of A. cervicornis cover at some sites, leaving populations sparsely distributed throughout its range and typically found as isolated colonies. Even though populations are depauperate causing a decrease in sexual reproduction, its fast growth rate and ability to reproduce through asexual fragmentation affords this species the potential for quick recovery and population growth. However, limited to no natural recovery has been documented. Many of these populations are poorly studied …

Genetic Population And Evolutionary Dynamics Of The Angel Sharks, Squatina Spp., Cristin Keelin Fitzpatrick

HCNSO Student Theses and Dissertations

Once so abundant as to be called the ‘common’ angelshark, Squatina squatina has been extirpated from nearly the entirety of its historical range, from the eastern North Atlantic, to the Mediterranean Sea [International Union for the Conservation of Nature (IUCN) Red List: Critically Endangered]. The angelshark now only occurs in any abundance in the waters surrounding the Canary Islands. I present the first genetic assessment of the angelshark’s population dynamics and diversity from three locations within the Canary Islands archipelago: Gran Canaria, Tenerife, and Lanzarote. Using a suite of individual mitochondrial genome regions [Control region (CR), NADH dehydrogenase subunit 2 …

Biological And Abiotic Factors Influencing Cetacean Mass Strandings In The Northeastern United States, Octavia Williams

HCNSO Student Capstones

Marine mammal strandings are frequent occurrences along many coastal areas around the world. The significance of stranded cetaceans has been of interest since around 300 B.C. when philosophers, like Aristotle, pondered why marine mammals, including whales, dolphins and porpoise beach themselves; and famous authors, like Henry David Thoreau, whose fascination in mammal strandings lead him to write a book about these mass occurrences off the coast of Cape Cod. Numerous hypothetical theories have been developed to explain these mass stranding phenomena. Though the causes of mass strandings remain unresolved, recent investigations suggest contributing factors could include environmental elements. Less emphasis …

Trophic Ecology And Parasitism Of A Mesopelagic Fish Assemblage, Matthew Woodstock

HCNSO Student Theses and Dissertations

Mesopelagic (open ocean, 200-1000 m depth) fishes are important consumers of zooplankton and are prey of oceanic predators. Some mesopelagic fishes (e.g., myctophids and stomiids) undertake a diel vertical migration where they ascend to the near-surface waters during the night to feed and descend into the depths during the day to avoid predators. Other mesopelagic fishes (e.g., Sternoptyx spp.) do not vertically migrate and remain at deep depths throughout the day. While in the epipelagic zone (surface – 200 m depth), vertically migrating fishes become prey to upper-trophic level predators, such as: tunas and billfishes. Benthic fishes (e.g., macrourids) often …

Identification And Spatiotemporal Dynamics Of Tuna (Family: Scombridae; Tribe: Thunnini) Early Life Stages In The Oceanic Gulf Of Mexico, Nina Pruzinsky

HCNSO Student Theses and Dissertations

Fishes within the family Scombridae (i.e. tunas, mackerels and bonitos) are of high ecological and economic value, as they are heavily targeted by commercial and recreational fisheries. In coastal and open-ocean environments, adults are high-level predators, while larvae and juveniles serve as prey for numerous species. Much is known about the distribution and abundance of adult tunas, but high taxonomic uncertainty and limited knowledge regarding the distributional patterns of larval and juvenile tunas have led to an “operational taxonomic unit” gap in our understanding of tuna ecology. Scombrids were collected across the Gulf of Mexico (GoM, hereafter) during seven research …

Neuropsychiatric Aspects Of Near-Death Experience, Jerod Buchta, Mir Saleem

Biology Faculty Articles

Throughout human history, there have been numerous reports of a baffling process when individuals have come to the brink of death. This process, referred to as near death experiences (NDEs), cause an individual to experience events that even the most advanced science can still not answer. This review serves as a reference for the factors, phenomenology, and measurement techniques for near death experiences as well as summarizing these experiences in certain conditions, such as cardiac arrest and admission of anesthetics. Discussed are the theories, effects, analyses of specific processes involved pertaining to experiences of those who walked along the edge …

Internal Validation Of Strmix™ – A Multi Laboratory Response To Pcast, Jo-Anne Bright, Rebecca Richards, Maarten Kruijver, Hannah Kelly, Catherine Mcgovern, Alan Magee, Andrew Mcwhorter, Anne Cieko, Brian Peck, Chase Baumgartner, Christina Buettner, Scott Mcwilliams, Claire Mckenna, Colin Gallacher, Ben Mallinder, Darren Wright, Deven Johnson, Dorothy Catella, Eugene Lien, Craig O'Connor, George Duncan, Jason Bundy, Jillian Echard, John Lowe, Joshua Stewart, Kathleen Corrado, Sheila Gentile, Marla Kaplan, Michelle Hassler, Naomi Mcdonald, Paul Hulme, Rachel H. Oefelein, Shawn Montpetit, Melissa Strong, Sarah Noel, Simon Malsom, Steven Myers, Susan Welti, Tamyra Moretti, Teresa Mcmahon, Thomas Grill, Tim Kalafut, Mary Margaret Greer-Ritzheimer, Vickie Beamer, Duncan A. Taylor, John S. Buckleton

Biology Faculty Articles

We report a large compilation of the internal validations of the probabilistic genotyping software STRmix™. Thirty one laboratories contributed data resulting in 2825 mixtures comprising three to six donors and a wide range of multiplex, equipment, mixture proportions and templates. Previously reported trends in the LR were confirmed including less discriminatory LRs occurring both for donors and non-donors at low template (for the donor in question) and at high contributor number. We were unable to isolate an effect of allelic sharing. Any apparent effect appears to be largely confounded with increased contributor number.

Periodontitis In Patients With Type 2 Diabetes Mellitus, Simran Agarwal, Deepthi Gangaram, Mir Saleem

Biology Faculty Articles

Periodontitis is a chronic, inflammatory disease that is characterized by swollen gums, degradation of jaw structure, and potentially tooth loss. Like Periodontitis, Type 2 Diabetes Mellitus is also highly prevalent and largely multifactorial. Type 2 Diabetes is a condition in which higher than normal blood glucose levels cannot be properly maintained as the body’s normal mechanism of maintaining proper blood glucose levels, the release of insulin from pancreatic islet β-cells, becomes ineffective. The purpose of this literature review is to explore the relationship between the incidences of Periodontitis and Type 2 Diabetes. The risk that Type 2 Diabetes poses for …

The Effect Of Social Support On Self-Care For Patients With Diabetes, Avidor Gerstenfeld

Mako: NSU Undergraduate Student Journal

No abstract provided.

The Role Of Ontogenetic Habitat Shifts On The Parasite Communities Of Five South Florida Fishes, Brittany Nicole White

HCNSO Student Theses and Dissertations

Many reef fishes initially recruit into mangroves, and then migrate out to reef habitats as they grow and mature. Each ontogenetic habitat shift exposes migrants to previously unencountered parasite taxa, potentially increasing parasite species richness and driving changes in parasite community structure. However, studies on this topic rarely attempt to distinguish between the location effects of habitat shifts versus a simple increase in physical size. Therefore we contrasted parasite community richness and structure in Great Barracuda Sphyraena barracuda (N=84), Atlantic Needlefish Strongylura marina (N=49), Crevalle Jack Caranx hippos (N=59), White Mullet Mugil curema (N=90), and Yellow-fin Mojarra Gerres cinnerus (N=60) …

Pathogen Transmission Techniques And Genotypic Resistance To Disease In The Threatened Coral, Acropora Cervicornis, Megan Bock

HCNSO Student Theses and Dissertations

Unprecedented population losses of the staghorn coral, Acropora cervicornis, since the 1970s have been attributed primarily to disease. Although a positive linear relationship between disease prevalence and increased water temperature has been described, the pathogen(s) causing disease and whether they are spread through the water or vectors is still poorly understood. Additionally, an increase in disease outbreaks and severity has provided an urgent need to identify natural genotypic resistance to disease in Caribbean acroporids. Studies to date have explored a variety of pathogen transmission methods, but prior to this study, there has been no examination of differences among common techniques. …

Identifying Disease-Resistant And Thermal-Tolerant Genotypes In The Threatened Staghorn Coral, Acropora Cervicornis, Morgan V. Hightshoe

HCNSO Student Theses and Dissertations

Since the 1970s, loss of herbivores, coral bleaching, pollution, and disease epidemics have reshaped the ecological framework of coral reefs. Staghorn coral, Acropora cervicornis, was a major reef-building scleractinian coral found throughout Florida and the Caribbean that experienced unprecedented population declines primarily due to disease and coral bleaching. These two stressors are coupled; the highest coral disease prevalence occurs after periods of thermal stress caused by increased sea surface temperature. Previous research documented three disease-resistant A. cervicornis genotypes in Panama, but it is unknown if disease-resistant genotypes exist in the Florida Keys. Thermal tolerance has been found to be …

Toxicological Effects Of Commercial Sunscreens On Coral Reef Ecosystems: New Protocols For Coral Restoration, Emilie C. Johnsen

HCNSO Student Capstones

The primary purpose of consumer-grade sunscreen is to protect skin from harmful UVA and UVB rays. This market has grown during the past 80 years, and environmental contamination from increasing amounts of sunscreen compounds have created concern. In particular, impacts on ocean ecosystems have inspired investigations and toxicological research on their effects on marine life. Unfortunately, such studies using marine flora and fauna are scarce, and the impact of chemical exposure to consumer sunscreens is neither adequately measured nor completely understood. In a pilot study by the Coral Restoration Foundation, in situ toxicity exposure to 10 different brands of sunscreens …

Southeast Florida Reef-Wide Post-Irma Coral Disease Surveys, Brian K. Walker

Marine & Environmental Sciences Faculty Reports

Florida’s coral reefs are currently experiencing a multi-year outbreak of coral disease that have resulted in the mortality of millions of corals across southeast Florida, Biscayne National Park, and the Upper and Middle Florida Keys. In early September 2017, Hurricane Irma impacted the entire FRT. The purpose of this project was to conduct field surveys to identify the current state of the coral reefs in southeast Florida and coordinate with other concomitant reef tract efforts to improve the regional understanding of the extent of the disease outbreak and identify recent hurricane injury to direct future restoration. Through a broader partner …

Age And Growth Of Hogfish (Lachnolaimus Maximus) In Southeast Florida, Ian A. Towne

HCNSO Student Theses and Dissertations

Hogfish (Lachnolaimus maximus; Walbaum 1792) from Southeast Florida were aged using sectioned otoliths and growth rates were calculated using the von Bertalanffy growth equation. The samples were collected from Broward County (n=209); other regions of Southeast Florida (n=18), the Florida Keys (n=35) and Bahamas (n=43). Growth rates were determined for each of these areas and were then compared to previously reported growth rates from other regions including the eastern Gulf of Mexico and Florida Keys. There was significant separation at the 95% confidence level between growth rates from each reagion. The average maximum fork length increased, from the Florida …

The Successful Exploitation Of Urban Environments By The Golden Silk Spider, Nephila Clavipes (Araneae, Nephilidae), Jake Ripp, Omar T. Eldakar, Andrew C. Gallup, Paul T. Arena

Biology Faculty Articles

Urbanization typically leads to habitat destruction producing negative effects for native species, but some species may exploit these settings. This concept was investigated in the golden silk spider (Nephila clavipes), a large, formidable spider that commonly inhabits forest edges as well as open spaces in urban environments throughout its vast geographic range. Here, we compared variation of N. clavipes success as measured by body size, web size and web positioning along an urban–rural gradient in southern Florida. From morphological measurements collected in the field, urban spiders had 60% longer legs and 35% longer bodies than both park and …

Marker Of Proliferation Ki-67 Expression Is Associated With Transforming Growth Factor Beta 1 And Can Predict The Prognosis Of Patients With Hepatic B Virus-Related Hepatocellular Carcinoma, Chengkun Yang, Hao Su, Xiwen Liao, Chuangye Han, Tingdong Yu, Guangzhi Zhu, Xiangkun Wang, Cheryl Ann Winkler, Stephen J. O’Brien, Tao Peng

Biology Faculty Articles

Hepatocellular carcinoma (HCC) is the most frequent malignancy of the liver. Transforming growth factor beta 1 (TGFB1) and marker of proliferation Ki-67 (MKI67) regulate cell proliferation, differentiation, and growth. The association between MKI67 and TGFB1 expression and its clinical implications in HCC remain unknown. Methods: Public databases were used to analyze TGFB1 and MKI67 expression in different pathologic grades/stages and tissue types of HCC. The association between MKI67 and TGFB1 expression was explored using pathway analysis and in a HepG2 cell line treated with TGFB1. Survival analysis was performed to evaluate the prognostic value of TGFB1 and MKI67 expression in …

Case Study Disaster Case Description: Public Health, Halmos College Of Arts And Sciences (Hcas)

HCAS Case Competitions

No abstract provided.

A New Species Of Cybianthus (Myrsinaceae) From The Cordillera Del Condor (Ecuador And Peru), John J. Pipoly Iii, Jon M. Ricketson

Marine & Environmental Sciences Faculty Articles

Cybianthus condorensis Pipoly & Ricketson, a new species from the Cordillera del Condor of Ecuador and Peru, is described herewith. Because of its alternate, coriaceous, and revolute leaves; scattered to densely appressed lepidote branchlets, inflorescence rachises, and calyces; subsessile pistillate flowers; and sandy habitat, this species is easily recognized.

Collaboration, Creativity, Compromise, And Conclusion: U.S. Coast Guard Modifies The Port Of Miami Anchorage Area, Paul D. Lehmann

Marine & Environmental Sciences Faculty Proceedings, Presentations, Speeches, Lectures

No abstract provided.