Life Sciences Commons^™

Serologic And Hematologic Values Of Bison In Colorado, Edward O. Keith, James Ellis, Robert W. Phillips, Maxine M. Benjamin

Marine & Environmental Sciences Faculty Articles

Recent economic and aesthetic interest in North American bison (Bison bison) has lead to increased interstate transport of these animals. Serologic and hematologic standards for bison are needed to detect disease in transported animals as well as within herds. This paper describes variation in blood physiological parameters in bison caused by variations in diet and season. Blood was taken from six bison and analyzed for serologic and hematologic parameters. Significant variation was found in blood urea nitrogen, chloride, cholesterol, creatinine, eosinophil, glucose, hemoglobin, lactic dehydrogenase, leukocyte, packed cell volume, potassium, serum globulin, serum glutamic oxalacetic transaminase, SGPT, and …

Bvr-1, A Restriction Locus Of A Type C Rna Virus In The Feline Cellular Genome: Pleiotropic Restriction Of Endogenous Balb Virus In Cat X Mouse Somatic Cell Hybrids, Stephen J. O'Brien, Janice M. Simonson

Biology Faculty Articles

Bvr-1 is a dominant X-linked feline gene which restricts the replication of B-tropic murineleukemia virus (B-MuLV) in somatic cell hybrids between murine BALB/c-RAG cells and FL-74 feline cells. Since the hybrids were originally derived by the hypoxanthine aminopterin thymidine selection scheme, counter selection experiments on 6-thioguanine result in preferential survival of hybrid cells which have spontaneously lost the feline X-chromosome on which is located the structural gene for hypoxanthine guanine phosphoribosyl transferase (IMP: pyrophosphate phosphoribosyl transferase, E.C. 2.4.2.8) and Bvr-1. Back selected Bvr-1^- cells express high parental levels of B-MuLV. Bvr-1^- effectively restricts the IdU-mediated induction of …

Climatic Implications Of Barbados Coral Growth, Richard E. Dodge, Karl K. Turekian, J. Rimas Vaisnys

Marine & Environmental Sciences Faculty Proceedings, Presentations, Speeches, Lectures

Results from a coral growth band analysis utilizing samples of M. annularis from the Recent Barbados reef and from three fossil raised reefs (Barbados I, II, and III dated at 82,000, 105,000, and 125,000 yrs. B.P.) indicate that in the Barbados II collection both average band width and variability were lower than in the other samples. We suggest the climate during formation of the 105,000 yrs. B.P. reef was significantly different than that of the present.

Coral Populations And Growth Patterns: Responses To Sedimentation And Turbidity Associated With Dredging, Richard E. Dodge, J. Rimas Vaisnys

Marine & Environmental Sciences Faculty Articles

Analysis of coral growth patterns and populations in Bermuda reveals that living coral abundance on the reefs of Castle Harbor, a location where extensive dredging occurred during 1941- 1943, is much reduced in comparison to external North-South reefs. Dead corals, sampled in the harbor, have skeletal patterns of growth which are similar and which show a marked decline in growth for several years prior to death. For the brain coral genus Diploria, both D. strigosa and D. labyrinthiformis are fairly evenly represented on contemporary North-South reefs and in the assemblages of dead corals from Castle Harbor, while D. labyrinthiformis, …

Bvr-1, A Restriction Locus Of A Type C Rna Virus In The Feline Cellular Genome: Identification, Location, And Phenotypic Characterization In Cat X Mouse Somatic Cell Hybrids, Stephen J. O'Brien

Biology Faculty Articles

Somatic cell hybrids were constructed between BALB/c-RAG mouse cells and feline lymphoma cells by the hypoxanthine-aminopterin-thymidine selection scheme. RAG cells spontaneously produce an endogenous B-tropic type C virus. Cat-mouse hybrids preferentially segregate feline chromosomes and retain murine chromosomes,demonstrable by karyotypic and isozyme analyses. Despite the presence of the complete mouse genome, including the viral genome, virus production was diminished to 1-5% of the levels observed in RAG parents based upon particle-associated RNA-dependent DNA polymerase (reverse transcriptase) activity in the culture fluid. Thirty-seven hybrids made on four different occasions had suppressed virus levels, and no hybrids expressed parental virus levels. Reverse …

Shell Growth In The Scallop Argopecten Irradians. I. Isotope Incorporation With Reference To Diurnal Growth, Alfred P. Wheeler, Patricia Blackwelder, Karl M. Wilbur

Marine & Environmental Sciences Faculty Articles

1. Incorporation of calcium and carbonate into shell has been studied in the scallop Argopecten irradians using ⁴⁵Ca and ¹⁴C-bicarbonate.

2. The incorporation of ⁴⁵Ca and ¹⁴C-carbonate into shell was linear with time after a lag period of 1 to 2 hours. The shell-forming mantle tissue attained a steady state with respect to ⁴⁵Ca in the sea water medium within 2 hours.

3. The molar ratio of ⁴⁵Ca to ¹⁴C-carbonate deposited in shell was not significantly different from unity during 5 hours.

4. The rate of incorporation of ¹⁴C-carbonate into shell was …

The Α-Glycerophosphate Cycle In Drosophila Melanogaster Iv. Metabolic, Ultrastructural, And Adaptive Consequences Of Αgpdh-1 "Null" Mutations, Stephen J. O'Brien, Yoshio Shimada

Biology Faculty Articles

"Null" mutations previously isolated at the αGpdh-1 locus of Drosophila melanogaster, because of disruption of the energy-producing α-glycerophosphate cycle, severely restrict the flight ability and relative viability of affected individuals. Two "null" alleles, αGpdh-1^BO-1-4, and αGpdh-1^BO-1-5, when made hemizygous with a deficiency of the αGpdh-1 locus,Df(2L)GdhA, were rendered homozygous by recombination with and selective elimination of the Df(2L)GdhA chromosome. After over 25 generations, a homozygous αGpdh-1^BO-1-4stock regained the ability to fly despite the continued absence of …

The Α-Glycerophosphate Cycle In Drosophila Melanogaster. Iii. Relative Viability Of "Null" Mutants At The Α-Glycerophosphate Dehydrogenase-1 Locus, Stephen J. O'Brien, Bruce Wallace, Ross J. Macintyre

Biology Faculty Articles

No abstract provided.

An Analysis Of Gene-Enzyme Variability In Natural Populations Of Drosophila Melanogaster And D. Simulans, Stephen J. O'Brien, Ross J. Macintyre

Biology Faculty Articles

Nine populations of D. melanogaster and two populations of D. simulans were analyzed for polymorphism in 10 gene-enzyme systems by the technique of gel electrophoresis. In the eight natural populations of D. melanogaster, an average of 54% of the enzymes were polymorphic, and the average heterozygosity was 22.7%. An experimental population of D. melanogaster, which has been maintained in a laboratory cage for 20 years, showed levels of polymorphism equivalent to those of natural populations. The D. simulans populations had much less variability. The possible factors involved in maintaining these polymorphisms are discussed.