Life Sciences Commons^™

387. Gene Delivery Of Apoe2 Reduces Amyloid Pathology In Transgenic Mouse Models Of Alzheimer's Disease, Lingzhi Zhao, Andrew J. Gottesdiener, Mayur S. Parmar, Christine Grevstad, David Havlicek, Jonathan Rosenberg, Stephen Kaminsky, Maria Chiuchiolo, Dolan Sondhi, Ronald G. Crystal, Steven M. Paul

HPD Articles

The deposition of amyloid β-peptides (Aβ), cleavage products of the amyloid precursor protein (APP) by β- and γ-secretases, in brain represents a pathological hallmark of Alzheimer's disease (AD). Apolipoprotein E (APOE) ε4 allele carriers have an increased risk to develop AD and an earlier age of onset, whereas carriers of the ε2 allele have reduced risk and a delayed age of onset. APOE is also a major determinant of brain Aβ and amyloid burden in humans and in several transgenic mouse models of AD (E4>E3>E2). We have previously reported that lentivirus-mediated intraparenchymal gene delivery of APOE2 significantly reduces …

A Dynamic Behavioral Biometric Approach To Authenticate Users Employing Their Fingers To Interact With Touchscreen Devices, Arturo Ponce

CCAC Theses and Dissertations

The use of mobile devices has extended to all areas of human life and has changed the way people work and socialize. Mobile devices are susceptible to getting lost, stolen, or compromised. Several approaches have been adopted to protect the information stored on these devices. One of these approaches is user authentication. The two most popular methods of user authentication are knowledge based and token based methods but they present different kinds of problems.

Biometric authentication methods have emerged in recent years as a way to deal with these problems. They use an individual’s unique characteristics for identification and have …

Mixed Ancestry And Admixture In Kauai's Feral Chickens: Invasion Of Domestic Genes Into Ancient Red Junglefowl Reservoirs, Eben Gering, M. Johnsson, P. Willis, T. Getty, D. Wright

Biology Faculty Articles

A major goal of invasion genetics is to determine how establishment histories shape non‐native organisms' genotypes and phenotypes. While domesticated species commonly escape cultivation to invade feral habitats, few studies have examined how this process shapes feral gene pools and traits. We collected genomic and phenotypic data from feral chickens (Gallus gallus) on the Hawaiian island of Kauai to (i) ascertain their origins and (ii) measure standing variation in feral genomes, morphology and behaviour. Mitochondrial phylogenies (D‐loop & whole Mt genome) revealed two divergent clades within our samples. The rare clade also contains sequences from Red Junglefowl (the …

A New Sustainable Environmental Education Toolbox For Botanic Gardens, Parks & Arboreta, John J. Pipoly Iii

Marine & Environmental Sciences Faculty Proceedings, Presentations, Speeches, Lectures

No abstract provided.

Hyperdominance In Amazonian Forest Carbon Cycling, Sophie Fauset, Michelle O. Johnson, Manuel Gloor, Timothy R. Baker, Abel Monteagudo M., Roel J. W. Brienen, Ted R. Feldpausch, Gabriela Lopez-Gonzalez, Yadvinder Malhi, Hans Ter Steege, Nigel C. A. Pitman, Christopher Baraloto, Julien Engel, Pascal Petronelli, Ana Andrade, Jose Luis C. Camargo, Susan G. W. Laurance, William F. Laurance, Jerome Chave, Elodie Allie, Percy Nunez Vargas, John W. Terborgh, Kalle Ruokolainen, Marcos Silveira, Gerardo A. Aymard C., Luzmila Arroyo, Damien Bonal, Hirma Ramirez-Angulo, Alejandro Araujo-Murakami, David Neill, Bruno Herault, Aurelie Dourdain, Armando Torres-Lezama, Beatriz S. Marimon, Rafael P. Salomao, James A. Comiskey, Maxime Rejou-Mechain, Marisol Toledo, Juan Carlos Licona, Alfred Alarcon, Adriana Prieto, Agustin Rudas, Peter J. Van Der Meer, Timothy J. Killeen, Ben-Hur Marimon Jr., Lourens Poorter, Rene G. A. Boot, Basil Stergios, Emilio Vilanova Torre, Flavia R. C. Costa, Carolina Levis, Juliana Schietti, Priscila Souza, Nikee Groot, Eric Arets, Victor Chama Moscoso, Wendeson Castro, Euridice N. Honorio Coronado, Marielos Pena-Claros, Clement Stahl, Jorcely Barroso, Joey Talbot, Ima Celia Guimaraes Vieira, Geertje Van Der Heijden, Raquel Thomas, Vincent A. Vos, Everton C. Almeida, Esteban Alvarez Davila, Luiz E. O. C. Aragao, Terry L. Erwin, Paulo S. Morandi, Edmar Almeida De Oliveira, Marco B. X. Valadao, Roderick J. Zagt, Peter Van Der Hout, Patricia Alvarez Loayza, John J. Pipoly Iii, Ophelia Wang, Miguel Alexiades, Carlos E. Ceron, Isau Huamantupa-Chuquimaco, Anthony Di Fiore, Julie Peacock, Nadir C. Pallqui Camacho, Ricardo K. Umetsu, Plinio Barbosa De Camargo, Robyn J. Burnham, Rafael Herrera, Carlos A. Quesada, Juliana Stropp, Simone A. Vieira, Marc Steininger, Carlos Reynel Rodriguez, Zorayda Restrepo, Adriane Esquivel Muelbert, Simon L. Lewis, Georgia C. Pickavance, Oliver L. Phillips

Marine & Environmental Sciences Faculty Articles

While Amazonian forests are extraordinarily diverse, the abundance of trees is skewed strongly towards relatively few ‘hyperdominant’ species. In addition to their diversity, Amazonian trees are a key component of the global carbon cycle, assimilating and storing more carbon than any other ecosystem on Earth. Here we ask, using a unique data set of 530 forest plots, if the functions of storing and producing woody carbon are concentrated in a small number of tree species, whether the most abundant species also dominate carbon cycling, and whether dominant species are characterized by specific functional traits. We find that dominance of forest …

Dissipation Rate Of Turbulent Kinetic Energy In Diel Vertical Migrations: Comparison Of Ansys Fluent Model To Measurements, Cayla Whitney Dean, Alexander Soloviev, Amy Hirons, Tamara M. Frank, J. D. Wood

Marine & Environmental Sciences Faculty Proceedings, Presentations, Speeches, Lectures

Recent studies suggest that diel vertical migrations of zooplankton may have an impact on ocean mixing, though details are not completely clear. A strong sound scattering layer of zooplankton undergoing diel vertical migrations was observed in Saanich Inlet, British Colombia, Canada by Kunze et al. (2006). In this study, a shipboard 200- kHz echosounder was used to track vertical motion of the sound scattering layer, and microstructure profiles were collected to observe turbulence. An increase of dissipation rate of turbulent kinetic energy by four to five orders of magnitude was measured during diel vertical migrations of zooplankton in one case …

14th Annual Undergraduate Student Symposium, Farquhar Honors College

Undergraduate Student Symposium

The Undergraduate Student Symposium, sponsored by the Farquhar College of Arts and Sciences, presents student projects through presentations, papers, and poster displays. The event serves as a “showcase” demonstrating the outstanding scholarship of undergraduate students at NSU. The symposium is open to undergraduate students from all disciplines. Projects cover areas of student scholarship ranging from the experimental and the applied to the computational, theoretical, artistic, and literary. They are taken from class assignments and independent projects. The projects do not have to be complete; presentations can represent any stage in the concept’s evolution, from proposal and literature review to fully …

Estimating Growth And Mortality In Elasmobranchs: Are We Doing It Correctly?, Brian J. Moe

HCNSO Student Theses and Dissertations

The instantaneous mortality rate (M) is an important parameter in elasmobranch management and conservation, but is difficult to estimate directly. Thus, indirect estimates based on relatively easily obtained life history parameters are commonly used. Many indirect methods incorporate one or more parameters from the von Bertalanffy growth model (VBGM), which is often criticized for its inability to describe changes in growth associated with maturity. The Lester growth model (LGM) is a biphasic alternative to the VBGM that incorporates trade-offs between reproduction, growth, and survival, and may therefore more accurately estimate M. I used published data from 29 …

Seasonal Warm-Water Refuge And Sanctuary Usage By The Florida Manatee (Trichechus Manatus Latirostris) In Kings Bay, Citrus County, Florida, Danielle C. Sattelberger

HCNSO Student Theses and Dissertations

The largest Florida manatee (Trichechus manatus latirostris) aggregation at a natural warm-water refuge occurs in Kings Bay, Crystal River, FL. Over the last 32 years, the U.S. Fish and Wildlife Service and the State of Florida have created a network of manatee protection areas within Kings Bay including a year-round refuge designation and seven Federal manatee sanctuaries during the winter manatee season (November 15 – March 31). Aerial survey data collected between 1983 and 2012 was used to examine the seasonal change in manatee distribution within Kings Bay in order to assess the effectiveness of current sanctuary sizes …

Evaluation Of Endocrine Disrupting Chemicals In The Florida Coastal Pelagic Fish Complex Following The Deepwater Horizon Oil Spill Event, Rachel Hickey

HCNSO Student Theses and Dissertations

Following the BP Deepwater Horizon oil spill event, there was a critical need to assess the effects of the oil and dispersant chemicals on the coastal pelagic fish complex in the Gulf of Mexico and the Florida Straits. The objective of this study was to determine if spilled crude oil and dispersant chemicals have posed an ecological risk to the coastal pelagic fish complex through the detection of vitellogenesis. Crude oil containing polycyclic aromatic hydrocarbons (PAHs), dispersant chemicals and other estrogen-mimicking compounds are suspected to induce vitellogenin production in male and immature female fish, normally only produced by sexually mature …

Land-Based Coral Nurseries: A Valuable Tool For Production And Transplantation Of Acropora Cervicornis, Keri L. O'Neil

HCNSO Student Theses and Dissertations

Coral nurseries have become a popular and successful method to produce coral fragments for reef-restocking and restoration projects worldwide. Numerous in-situ coral nurseries have been established and many studies have focused on the most effective way to produce coral fragments in offshore nurseries. In contrast, production of coral fragments in land-based nurseries is rarely studied despite a growing knowledge of coral husbandry and coral aquaculture. Little data exist on the success of tank-raised corals when transplanted back into reef environments. This thesis presents the results of a study designed to assess the use of land-based coral nurseries in production of …

Histology And Ultrastructure Of Montastraea Cavernosa And Porites Astreiodes During Regeneration And Recruitment: Anthropogenic Stressors And Transplant Success, Dorothy-Ellen A. Renegar

HCNSO Student Theses and Dissertations

Corals combine photosynthesis and calcification in an intricate and delicately balanced relationship to form large biomineralized structures that are dominant features of tropical coastlines worldwide. Coral reefs have great scientific and economic importance but have recently experienced widespread decline attributed to increasing anthropogenic pressure on reef systems. Physical damage events, such as ship groundings, when coupled with existing nutrient stress and changing global climate present a poor outlook for successful natural recovery of reef communities. The main goal of the proposed research is to better understand how environmental factors, both local and global, affect the coral holobiont and influence overall …

Image Enhancement Of Cancerous Tissue In Mammography Images, Richard Thomas Richardson

CCAC Theses and Dissertations

This research presents a framework for enhancing and analyzing time-sequenced mammographic images for detection of cancerous tissue, specifically designed to assist radiologists and physicians with the detection of breast cancer. By using computer aided diagnosis (CAD) systems as a tool to help in the detection of breast cancer in computed tomography (CT) mammography images, previous CT mammography images will enhance the interpretation of the next series of images. The first stage of this dissertation applies image subtraction to images from the same patient over time. Image types are defined as temporal subtraction, dual-energy subtraction, and Digital Database for Screening Mammography …

Origins Of An Unmarked Georgia Cemetery Using Ancient Dna Analysis, Andrew T. Ozga, Raul Y. Tito, Brian M. Kemp, Hugh Matternes, Alexandra J. Obregon-Tito, Leslie Neal, Cecil M. Lewis Jr.

Biology Faculty Articles

Determining the origins of those buried within undocumented cemeteries is of incredible importance to historical archaeologists and, in many cases, the nearby communities. In the case of Avondale Burial Place, a cemetery in Bibb County, Georgia, in use from 1820 to 1950, all written documentation of those interred within it has been lost. Osteological and archaeological evidence alone could not describe, with confidence, the ancestral origins of the 101 individuals buried there. In the present study, we used ancient DNA extraction methods in well-preserved skeletal fragments from 20 individuals buried in Avondale Burial Place to investigate the origins of the …

Subsistence Strategies In Traditional Societies Distinguish Gut Microbiomes, Alexandra J. Obregon-Tito, Raul Y. Tito, Jessica Metcalf, Krithivasan Sankaranarayanan, Jose C. Clemente, Luke K. Ursell, Zhenjiang Zech Xu, Will Van Treuren, Rob Knight, Patrick M. Gaffney, Paul Spicer, Paul Lawson, Luis Marin-Reyes, Omar Trujillo-Villarroel, Morris Foster, Emilio Guija-Poma, Luzmila Troncoso-Corzo, Christina Warinner, Andrew T. Ozga, Cecil M. Lewis Jr.

Biology Faculty Articles

Recent studies suggest that gut microbiomes of urban-industrialized societies are different from those of traditional peoples. Here we examine the relationship between lifeways and gut microbiota through taxonomic and functional potential characterization of faecal samples from hunter-gatherer and traditional agriculturalist communities in Peru and an urban-industrialized community from the US. We find that in addition to taxonomic and metabolic differences between urban and traditional lifestyles, hunter-gatherers form a distinct sub-group among traditional peoples. As observed in previous studies, we find that Treponema are characteristic of traditional gut microbiomes. Moreover, through genome reconstruction (2.2–2.5 MB, coverage depth × 26–513) and functional …

Dynamics Of Marine Bacterial Community Diversity Of The Coastal Waters Of The Reefs, Inlets, And Wastewater Outfalls Of Southeast Florida, Alexandra Mandina Campbell, Jay Fleisher, Christopher D. Sinigalliano, James R. White, Jose V. Lopez

Marine & Environmental Sciences Faculty Articles

Coastal waters adjacent to populated southeast Florida possess different habitats (reefs, oceanic inlets, sewage outfalls) that may affect the composition of their inherent microbiomes. To determine variation according to site, season, and depth, over the course of 1 year, we characterized the bacterioplankton communities within 38 nearshore seawater samples derived from the Florida Area Coastal Environment (FACE) water quality survey. Six distinct coastal locales were profiled – the Port Everglades and Hillsboro Inlets, Hollywood and Broward wastewater outfalls, and associated reef sites using culture-independent, high-throughput pyrosequencing of the 16S rRNA V4 region. More than 227,000 sequences helped describe longitudinal taxonomic …

Dihydrolipoic Acid Conjugated Carbon Dots Accelerate Human Insulin Fibrillation, Sheba J. Kuruvilla, Shanghao Li, Lorenzo Sansalone, Blake Fortes, Ian Zheng, Patricia Blackwelder, Cyrus Pumilia, Miodrag Micic, Jhony Orbulescu, Roger M. Leblanc

Marine & Environmental Sciences Faculty Articles

Protein fibrillation is believed to play an important role in the pathology and development of several human diseases, such as Alzheimer’s disease, Parkinson’s disease and type 2 diabetes. Carbon dots (CDs), as a new type of nanoparticle have recently been extensively studied for potential biological applications, but their effects on protein fibrillation remain unexplored. In reality, any application in biological systems will inevitably have “contact” between proteins and CDs. In this study, human insulin was selected as a model protein to study the effects of CDs on protein fibrillation, as proteins may share a common mechanism to form fibrils. Hydrophobic …

Migration Strategies Vary In Space, Time, And Among Species In The Smallfish Metacommunity Of The Everglades, J. Matthew Hoch, Eric R. Sokol, Aaron D. Parker, Joel C. Trexler

Marine & Environmental Sciences Faculty Articles

Spatial ecology and movement strategies of aquatic organisms may limit their response to human-caused drying of wetland habitats. We characterized the movement strategies of the most abundant species of fish in the wetlands of the Everglades (USA) to better understand how they cope with annual fluctuations in aquatic habitat size. Over a six-year period, we used a sampling method designed to measure the density, activity levels, and movement direction of small fishes. We estimated changes in displacement speed and directional bias to identify patterns of movement that different fishes use to disperse over the gradient of disturbance in this environment. …

3d Dynamics Of Freshwater Lenses In The Near-Surface Layer Of The Tropical Ocean, Alexander Soloviev, Cayla Whitney Dean, Atsushi Fujimura

Marine & Environmental Sciences Faculty Articles

Convective rains in the Intertropical Convergence Zone produce lenses of freshened water on the ocean surface. Due to significant density differences between the freshened and saltier seawater, strong pressure gradients develop, resulting in lateral spreading of freshwater lenses in the form of gravity currents. Gravity currents inherently involve three-dimensional dynamics. As a type of organized structure, gravity currents may also interact with, and be shaped by, the ambient oceanic and atmospheric environment. Among the important environmental factors are background stratification and wind stress. Under certain conditions, a resonant interaction between a propagating freshwater lens and internal waves in the underlying …

A Catch Per Unit Effort (Cpue) Spatial Metric With Respect To The Western North Atlantic Pelagic Longline Fishery, Max Appelman

HCNSO Student Theses and Dissertations

Catch per unit effort (CPUE) is a quantitative method used to describe fisheries worldwide. CPUE can be presented as number of fish per 1000 hooks, number of fish per amount of fishing time, or with any unit of effort that best describes the fishery (e.g., search time, hooks per hour, number of trawls). CPUE is commonly used as an index to estimate relative abundance for a population. These indices are then applied within stock assessments so that fisheries managers can make justified decisions for how to manage a particular stock or fishery using options such as quotas, catch …

Computer Modeling The Incursion Patterns Of Marine Invasive Species, Matthew W. Johnston

HCNSO Student Theses and Dissertations

Abstract Not Available.

Recurrent Evolution Of Melanism In South American Felids, Alexsandra Schneider, Corneliu Henegar, Kenneth Day, Devin Absher, Constanza Napolitano, Leandro Silveira, Victor A. David, Stephen J. O'Brien, Marilyn Menotti-Raymond, Gregory S. Barsh, Eduardo Eizirik

Biology Faculty Articles

Morphological variation in natural populations is a genomic test bed for studying the interface between molecular evolution and population genetics, but some of the most interesting questions involve non-model organisms that lack well annotated reference genomes. Many felid species exhibit polymorphism for melanism but the relative roles played by genetic drift, natural selection, and interspecies hybridization remain uncertain. We identify mutations of Agouti signaling protein (ASIP) or the Melanocortin 1 receptor (MC1R) as independent causes of melanism in three closely related South American species: the pampas cat (Leopardus colocolo), the kodkod (Leopardus guigna), and Geoffroy’s cat …

Biogeochemical Analysis Of Ancient Pacific Cod Bone Suggests Hg Bioaccumulation Was Linked To Paleo Sea Level Rise And Climate Change, Maribeth S. Murray, C. Peter Mcroy, L. K. Duffy, Amy Hirons, J. M. Schaaf, Robert P. Trocine, John Trefry

Marine & Environmental Sciences Faculty Articles

Deglaciation at the end of the Pleistocene initiated major changes in ocean circulation and distribution. Within a brief geological time, large areas of land were inundated by sea-level rise and today global sea level is 120 m above its minimum stand during the last glacial maximum. This was the era of modern sea shelf formation; climate change caused coastal plain flooding and created broad continental shelves with innumerable consequences to marine and terrestrial ecosystems and human populations. In Alaska, the Bering Sea nearly doubled in size and stretches of coastline to the south were flooded, with regional variability in the …

Water Column Productivity And Temperature Predict Coral Reef Regeneration Across The Indo-Pacific, Bernhard Riegl, Peter W. Glynn, Evie A. Wieters, Samuel J. Purkis, C. D'Angelo, Joerg Wiedenmann

Marine & Environmental Sciences Faculty Articles

Predicted increases in seawater temperatures accelerate coral reef decline due to mortality by heat-driven coral bleaching. Alteration of the natural nutrient environment of reef corals reduces tolerance of corals to heat and light stress and thus will exacerbate impacts of global warming on reefs. Still, many reefs demonstrate remarkable regeneration from past stress events. This paper investigates the effects of sea surface temperature (SST) and water column productivity on recovery of coral reefs. In 71 Indo-Pacific sites, coral cover changes over the past 1-3 decades correlated negative-exponentially with mean SST, chlorophyll a, and SST rise. At six monitoring sites …

Fdou Project 26a Part 5 Task 1 - Our Florida Reefs (Ofr) Survey Outreach Efforts, Brian K. Walker, Amanda Rose Costaregni

Marine & Environmental Sciences Faculty Reports

This report contains summaries of the OFR survey outreach activities completed in Miami-

Dade, Broward, Palm Beach, and Martin counties between October 1st, 2014 and February

2nd, 2015.

A total of seven presentations were given in Broward, Palm Beach, and Martin Counties.

These presentations were for groups such as dive clubs, underwater photography clubs, fishing clubs, and environmental professionals clubs. Unfortunately, due to scheduling conflicts no survey presentations have been given in Miami-Dade County to-date. Summaries of these outreach presentations include the name of the club, location of the presentation, number of people reached, demographics, questions and responses during the …

From The Editors, Eric Mason, Claire Lutkewitte

Quadrivium: A Journal of Multidisciplinary Scholarship

No abstract provided.

Butterfly Biology, Joshua Feingold

Quadrivium: A Journal of Multidisciplinary Scholarship

A photographic exploration of butterflies from larvae to adults in Davie, Florida.

Using The West Indian Manatee (Trichechus Manatus) As A Mechanism For Invasive Aquatic Plant Management In Florida, Aarin Conrad Allen, Edward O. Keith

Marine & Environmental Sciences Faculty Articles

West-Indian manatees (Trichechus manatus L.) are opportunistic, herbivorous aquatic mammals that occupy the warm, shallow coastal waters throughout the southeastern United States. Manatees are known to feed on large quantities of diverse plant types. Presently within the state of Florida, manatees are an endangered species facing environmental and anthropogenic threats. Several different organizations work to rescue and rehabilitate these animals for an eventual return to the wild. Also within Florida, invasive aquatic plants are becoming increasingly problematic, creating both negative economic and environmental impacts. Each year, efforts are made to control these exotic plant species through several different …

The Intended And Unintended Effects Of Fishing On Deep Sea Fish, David M. Bailey, Rosanna Milligan

Marine & Environmental Sciences Faculty Proceedings, Presentations, Speeches, Lectures

No abstract provided.

Smilefinder: A Resampling-Based Approach To Evaluate Signatures Of Selection From Genome-Wide Sets Of Matching Allele Frequency Data In Two Or More Diploid Populations, Wilfred M. Guiblet, Kai Zhao, Stephen J. O'Brien, Steven E. Massey, Alfred L. Roca, T. K. Oleksyk

Biology Faculty Articles

Background: Adaptive alleles may rise in frequency as a consequence of positive selection, creating a pattern of decreased variation in the neighboring loci, known as a selective sweep. When the region containing this pattern is compared to another population with no history of selection, a rise in variance of allele frequencies between populations is observed. One challenge presented by large genome-wide datasets is the ability to differentiate between patterns that are remnants of natural selection from those expected to arise at random and/or as a consequence of selectively neutral demographic forces acting in the population.

Findings: SmileFinder is …