Life Sciences Commons^™

Metal Release From Serpentine Soils In Sri Lanka, Meththika Vithanage, Anushka Upamali Rajapaksha, Christopher Oze, Nishanta Rajakaruna, C. B. Dissanayake

Biological Sciences

Ultramafic rocks and their related soils (i.e., serpentine soils) are non-anthropogenic sources of metal contamination. Elevated concentrations of metals released from these soils into the surrounding areas and groundwater have ecological-, agricultural-, and human health-related consequences. Here we report the geochemistry of four different serpentine soil localities in Sri Lanka by coupling interpretations garnered from physicochemical properties and chemical extractions. Both Ni and Mn demonstrate appreciable release in water from the Ussangoda soils compared to the other three localities, with Ni and Mn metal release increasing with increasing ionic strengths at all sites. Sequential extraction experiments, utilized to identify “elemental …

Assessing Rodent Species Counts And Diversity In The Not-Grazed Montana De Oro State Park And The Rotationally Grazed Pecho Ranch, Nancy Marie Lemos

Master's Theses

The available data examining the influence of rotational grazing on rodent responses are limited. This study investigated how rotational livestock grazing practices influence small mammal rodent abundances and species diversity. We looked for evidence of variation in the occurrence and/or numbers of certain rodent species among three plant community types (grassland, shrubland, grass-shrub mix), managed with or without grazing. We used Sherman live traps over a total of eight trapping sessions. We totaled 486 trapnights for each of the six plant community and grazing management combinations. The three plant community types were identified by visual cover.

Out of the eight …

Ultraviolet Led Biofouling Mitigation, Andrew Lam

Computer Engineering

The goal is to determine if low-cost UV LEDs can mitigate marine biofouling on small glass or acrylic camera lenses. A microprocessor-controlled experimental setup to control the illumination of low-cost UV LEDs of various wavelength and packaging was fashioned. The system consists of a programmed microcontroller, a manufactured LED breakout interface, and a submergible UV LED array enclosed in a borosilicate glass tube. A preliminary qualitative assessment of four different UV LEDs was conducted during a three-week deployment of the experimental setup in the raw seawater system at the Cal Poly Center for Costal and Marine Sciences in Avila Beach, …

Team Joseph: Adaptive Aquatic Device, Paul Sands, Lilly Hoff, Andrea Voigt

Mechanical Engineering

The scope of this project included designing and fabricating an adaptive aquatic device for Joseph, a 20 year old student in the Special Education Program at San Luis Obispo High School with a subset of cerebral palsy known as spastic quadriplegia. The project was presented at the beginning of the Fall 2013 quarter to the mechanical engineering students at Cal Poly with the aspiration that a team of engineers would construct a device that would allow Joseph, his friends and family to compete in their first triathlon on July 27, 2014. The project was humbly accepted by mechanical engineering students …

Monarch Butterfly (Danaus Plexippus) Tree Preference And Intersite Movement At California Overwintering Sites, Jessica Lynn Griffiths

Master's Theses

Managing Monarch butterfly overwintering groves: making room among the eucalyptus

Proper management and conservation of the coastal California overwintering sites used by western Monarch butterflies (Danaus plexippus L.) is critical for continued use of these sites by monarchs. Many management efforts are currently concentrating on eucalyptus-only sites because of the prevailing notion that monarchs prefer eucalyptus over native tree species. Yet, whether a preference exists or not has never been tested. Herein, we test the “eucalyptus preference” hypothesis with data from five overwintering sites comprised of blue gum eucalyptus (Eucalyptus globulus) and at least one other native tree …

The Role Of Sirtuin Inhibitors On The Proteomic Responses Of The Mussels Mytilus Galloprovincialis And Mytilus Trossulus To Menadione Induced Oxidative Stress, Hayley C. Chilton

Master's Theses

Global climate change imposes physiological constraints on marine ecosystems that can alter the distribution of intertidal organisms. In one such instance, the native cold-adapted mussel Mytilus trossulus is being replaced along its southern range by the invasive warm-adapted Mytilus galloprovincialis. These blue mussels occur throughout rocky intertidal zones where they are subjected to greatly varying environmental conditions known to induce oxidative stress. We hypothesize that while under acute stress, related Mytilus congeners undergo a shift in redox potential from NADH-fueled respiratory pathways to pathways producing NADPH as a way to decrease the production of reactive oxygen species (ROS) and …

Smooth Muscle Cell Organization In The Stem Region Of The Gracilis Collateral Circulation, Amanda Krall

Biomedical Engineering

Many patients who suffer from the ischemic Peripheral Arterial Occlusive Disease (PAOD) experience intermittent claudication, which can be attributed to impaired vasodilation. Collateral vessels are the primary site of resistance to blood flow downstream; therefore maximizing vasodilation in collaterals is crucial for efficient circulation. Collaterals function as natural bypasses around the occluded arteries and the increase in flow into these vessels causes them to outwardly remodel into conduit vessels. However, functional vasodilation in the stem region of collateral vessels is impaired at day 7 following femoral ligation, which can be attributed to smooth muscle cell malfunction. However, the increase in …

Bee Colony Startup And Cost Analysis, Marisela Herrera

BioResource and Agricultural Engineering

The increase in demand for European Honey bee pollination along with colony collapse disorder has caused bee pollination rental fees to greatly increase. This project intended to determine how effective and economically feasible it would be for almond growers to begin raising their own hives for pollination. It was determined the biggest challenges were organizing and finding employees who were knowledgeable about bee keeping. When looking at the cost it was clear that even with the current cost per hive the startup costs were still less than the total rental fees for a single pollination season.

Once the hives have …

Optimization Of Growth Parameters For Algal Regrowth Potential Experiments, Chadwick D. Boggess

Master's Theses

Water cost is one of the key deterrents in making algal biofuels a feasible alternative to traditional petroleum fuels. Research into recycling of algal media was conducted in a laboratory setting at the California Polytechnic State University in San Luis Obispo. A growth assay was created to allow for algal cultures, specifically Ward’s Chlorella sp., to proliferate in sealed culture vessels. Bold’s Basal Medium (BBM) was selected as the media due to its extensive use with freshwater algal strains. However, this medium can limit algal growth through nutrient limitation and it became necessary to augment the original recipe. 50mM …

Project Proposal And Development Plan For A New Security Gate At Swanton Pacific Ranch, K. Morgan Perry

Agricultural Education and Communication

The purpose of this project was to develop a prototype gate for Cal Poly’s Swanton Pacific Ranch in Santa Cruz, CA. Swanton Pacific Ranch requested that the author design and build a 21’x5’ single-retractable gate for Swanton Pacific Ranch to help decrease the security issues that have recently arisen at the facility. The design and format of this project are focused towards the desires and goals set forth by Swanton Pacific Ranch and the Operations Manager, Steve Auten in order to obtain a certain outcome. This project provides an outline of what needs to be done in order to produce …

Characterization Of Corynebacterium Pseudotuberculosis Biovar Equi Isolates Obtained From Cal Poly Horses And Review Of Application Of Transposons To Better Prevent Pigeon Fever In Horses, Irene Hasen

Animal Science

No abstract provided.

Beef Cattle Lesson Plans: For Use In The Education Of School-Age Children In The Beef Cattle Industry, Katherine M. Halloran, Cheyenne H. Love

Animal Science

This project was initially created for use at Fort Hope in Arroyo Grande, California. It designs lesson plans created to teach children about the beef cattle industry. The project consists of lesson plans for three age groups: 7-9, 10-12, and 13-15. Each age group will learn various aspects about beef cattle, and lessons will increase in complexity as the children advance age groups. The groups will spend six days in lecture completing worksheets and activities, and on the seventh day, after completion of a review game, the children receive certificates. While the project was created specifically for the Fort Hope …

The Effects Of Different Cryopreservation Techniques On The Survival Rate Of In Vitro Produced (Ivp) And Biopsied Bovine Blastocysts, Diana J. Gumber

Animal Science

This study’s objectives were to determine if the biopsy of expanded blastocysts prior to cryopreservation affected the survival rates and determine if there is a difference between slow-freezing in 10% glycerol, slow freezing in ethylene glycol, and vitrification in terms of survival rate of the embryos. Blastocoeles were reduced prior to cryopreservation by means of biopsy. Embryos for slow freezing were exposed to ethylene glycol (SynGRO Ethylene Glycol with Sucrose, BIONICHE, Pullman, WA, USA) or 10% glycerol (SynGRO 10% Glycerol, BIONICHE, Pullman, WA, USA) as cryoprotectants. Vitrified embryos were exposed to 10% and 40% ethylene glycol. Survival rate of biopsied …

Investigation Of Chromosome Size Effect On The Rate Of Crossovers In The Meiotic Yeast Saccharomyces Cerevisiae, Lanie Maria Galland

Master's Theses

Meiosis is a specialized type of cell division characterized by a single round of DNA replication and two rounds of chromosome segregation, ultimately resulting in four haploid cells. During meiosis I, chromosomes align and reciprocal recombination results in the formation of a crossover, creating the tension required to properly segregate homologs during the first round of meiosis.

Two mechanisms involved in regulating the occurrence of crossing over are assurance and interference. Crossover assurance describes the phenomenon that at least one crossover will form between each pair of homologous chromosomes during prophase I. Crossover interference, on the other hand, describes the …

Lamb Bacon: How The Method Of Brine Distribution And Addition Of A Dry Rub Influences Yields, Morgan Metheny

Animal Science

This study was conducted to test the whether lamb breasts can substitute as the raw material in producing a bacon product. Due to the difference in fat saturation between pork and lamb, this research was used to test how successful lamb absorbs the brine in order to make bacon. The yield results of two different processing techniques, injection and tumbling, were compared along with comparing the affect of adding a dry rub of seasonings to the lamb surface. The 120 lambs breasts were split into four groups of 30. Each group received a different treatment: plain tumbled, plain injected, seasoned …

Adaptive Basketball Shooter - Final Project Report, Jenna N. Becker, Richard A. Galanti, Justin D. Haley, Brett Y. Nayudu

Mechanical Engineering

The Friday Club is a joint venture between the Cal Poly Kinesiology Department and the San Luis Obispo Special Olympics that offers people with varying degrees of disability the opportunity to meet weekly and learn various sports and games. At Friday Club, athletes in wheelchairs with limited arm strength use devices built by Cal Poly mechanical engineering students in order to participate in various sports. Many devices are designed to attach to the Universal Play Frame (UPF), a wheel-chair attachment. The purpose of this project was to design and build a UPF device that will launch a basketball, so that …

An Examination Of An Environmental Change In Beverages Available To High School Students In Santa Maria, California On Their Consumption Behavior, Susan Eileen Klucker

Master's Theses

Hypothesis: "By an environmental change in high school vending machines, making water available, students will choose water over the sugar sweetened sodas."

This document highlights a case study analysis of vendor-provided refill data for forty-five beverage vending machines at two campus sites in one high school district in 2003 and 2004. The innovative study and publicized negotiated soda contract of a 50:50 (healthy to unhealthy) beverage ratio stipulation became the “Win-Win-WEAN” compromise, in which exposure to healthier beverage options for students might prove to provide the same income opportunity for the school district.

An overview of the political …

Photosynthetic Thermal Tolerance And Recovery To Short Duration Temperature Stress In Desert And Montane Plants: A Comparative Study, David William Gallagher

Master's Theses

• Climate change models predict an increase in frequency and amplitude of extreme weather events, including heat waves. To better predict how the composition and distribution of plant assemblages might respond to these changes in temperature, it is important to understand how species currently respond to these extremes. Photosynthetic thermal tolerance (T₂₅)and photosynthetic recovery (RT₂₅) were quantified in 27 species. We also studied the relationships between T₂₅, RT₂₅ and leaf mass per area (LMA). Leaf temperature was also monitored in the field.
• Leaves used in this study were collected from two distinct …

A Comparison Of The Standard 5-Day Co-Synch With Cidr Protocol And A 14-Day Co-Synch With Cidr Protocol In Primiparous And Multiparous Beef Cattle, Taylor Anne Dericco

Animal Science

No abstract provided.

Effect Of Grape-Leaf Potassium Content On Life-History Traits Of Willamette Spider Mite, Eotetranychus Willamettei (Mcgregor) (Acari: Tetranychidae), Michael Paul Sotelo

Horticulture and Crop Science

Vitis vinifera L. cv. Chardonnay vines were fertilized to approximate baseline, low, medium, and high grape-leaf potassium contents, relative to viticultural target values. From those vines, twelve replicates of leaf-discs were made per fertilizer treatment. The leaf-discs were populated with individuals of Willamette spider mite, Eotetranychus willamettei (McGregor) and maintained in a plant growth chamber (25°C, 14L:10D h). Data pertaining to maturation time (days), pre-oviposition period (days), lifetime fecundity (eggs / ♀), and longevity (days) of E. willamettei was recorded daily. Petiole analysis revealed that the grape-leaf potassium contents were greater than target values. Respective to fertilizer treatment, the actual …

Traversing Swanton Road, 15th Ed., James A. West

Academic, Government & Associated Organizational Research Resources

Situated at the northwest end of Santa Cruz County and occupying circa 30 square miles of sharply contrasted terrain, the Scott Creek Watershed concentrates within its geomorphological boundaries, at least 10-12% of California's flora, both native and introduced. Paradoxically, the use/abuse that the watershed has sustained over the past 140+ years, has not necessarily diminished the biodiversity and perhaps parallels the naturally disruptive but biologically energizing processes (fire, flooding, landslides and erosion), which have also been historically documented for the area for +60 years. This is an early edition of an extensive document by James A. West. Please visit the …

Teacher Internship Report - Anderson Union High School, Kathryn L. Teixeira

Agricultural Education: Graduate Internship Reports

This internship/project report includes validation documents required in meeting the quality criteria for secondary-level programs of instruction in agriculture. The documents are concurrently used for the Agriculture Incentive Grant review process at Anderson Union High School conducted by representatives of the California Department of Education. The internship included the practical application of occupational skills related to the horticulture industry and modification to the Greenhouse facilities at Anderson Union High School by improving ergonomics of greenhouse tables, replacing the existing siding of storage facilities and installing concrete walkways within the greenhouse. Students in the Agricultural Department were integral in all parts …

Learning Design Through The Lens Of Service: A Qualitative Study, David W. Hey, Lynne A. Slivovsky, Brian P. Self, James Widmann, J. Kevin Taylor

Kinesiology and Public Health

Twenty-four senior-level capstone engineering design projects were completed at a large, public, primarily undergraduate university involving 85 students (70 male and 15 female). All projects involved the design of equipment to facilitate physical activity for people with disabilities. The effects on: i) learning design, ii) attitude towards people with disabilities, iii) motivation to complete team design projects and iv) interdisciplinary collaboration were analyzed through 24 one-hour focus groups. We explored the student experience using a constructivist approach and grounded theory. Four major themes (with associated sub-themes) emerged from our data analysis: learning design (project management, iterative design process, and user-centered …

Student Apathy As Defined By Secondary Agricultural Education Students, Ann M. De Lay, Benjamin G. Swan

Agricultural Education and Communication

Student motivation continues to be a source of concern for educators. This phenomenological study captured the voices of secondary agriculture students as they shared their perspectives and experiences surrounding student apathy. Four focus group interviews were conducted at four central California high schools with distinguished agriculture programs. The following question guided the research: What experience do secondary agriculture students have with student apathy in their academic environments? Findings suggest student apathy is born of personal choice and grown through mediocre teaching, archaic assessment and the absence of learning purpose. Recommendations suggest students, teachers, the local school and teacher educators form …

A Preliminary Study Of The Role Of Nickel In Enhancing Flowering Of The Nickel Hyperaccumulating Plant Alyssum Inflatum Nyár. (Brassicaceae), R. Ghasemi, Z. Zare Chavoshi, R.S. Boyd, Nishanta Rajakaruna

Biological Sciences

Alyssum inflatum is a native of serpentine soils and is able to hyperaccumulate nickel (Ni), but the importance of Ni to reproduction in the species is unknown. We investigated if reproductive fitness is enhanced by Ni in the growth medium, and included a treatment involving a relatively high level of Mg to provide a comparison with elevated levels of another metal. Seedlings were grown in a modified Hoagland solution culture in an inert medium of Perlite and were treated with Ni (100 μM), a high concentration of Mg (5 mM), or under control conditions (solution culture without Ni or the …

Effects Of Covering System, Sealing Time, And Packing On Fermentation, Nutritional Quality, And Organic Matter Loss Of Corn Silage In A Drive-Over Pile, Katie Rose Nutcher

Dairy Science

This project involved an experiment conducted using a 550-ton capacity drive-over pile of whole-plant corn silage. The main effects compared were: packing the final forage surface with a loader or crawler, delay or immediate sealing, and covering with standard plastic or an oxygen barrier film.

Numerous studies have shown that the absence of oxygen in silage stored in a bunker silo or pile is crucial for proper fermentation and to ensure the highest quality silage at feed out. When oxygen is allowed to permeate through the covering material, it leads to visible spoilage. This trial showed that the oxygen barrier …

A Recommendation For The California Dairy System Reform Based On A Comparative Study Of Dairy Market Structuring And Pricing Systems Of Seven Leading Dairy Industries In 2014., Meredith Frisius

Dairy Science

No abstract provided.

Best Management Practices For Raising Dairy Calves From Birth To Weaning, Alexa Cabral

Dairy Science

There are many different ways to raise calves on dairy farms in the United States. The way calves are raised can depend on the resources, natural environment and operation of the farm. There is no right or wrong way to raise calves. However, the aim of raising calves is to find the best way to raise a healthy calf so that she will become a productive cow. The objective of this literature review was to determine the ideal management practices for raising a healthy calf from birth to weaning. This literature analyzed numerous articles that covered all aspects of calf …

Preparing Samples Of Rancid Milk For Milk Judging Contests, Danielle Li

Dairy Science

Due to the growing concern of raw milk, there is a growing need for other methods of preparing rancid milk for milk judging. The origin of rancidity in milk is due to lipolysis caused the lipase enzyme. However, with pasteurization, the lipase enzyme is inactivated. The purpose of this experiment was to compare the effect of using lipase powder to other current methods of preparing rancid milk, such as utilizing raw milk. Result showed that all treatments caused desired rancidity. The second purpose was to compare the rancidity between Method A, two to three hour incubation at room temperature, and …

Development Of Applied Standard Operating Procedures And Monitoring Practices, Nick Southfield

Dairy Science

Development of a standard operating procedure program for Southfield Dairy was chosen in order to improve the work environment and animal health with an underlying goal to improve profitability. Critical control points for the milk barns, maternity barn and calf barn on the dairy, were identified as being critical to the production of quality milk, both for sale and calf feed. Data recording implementation through identification of recordable data, equipment purchases, and log sheet production, was done so to increase relevant information available for veterinary services and specialty consultants. Implementation of procedural documents and training programs are discussed to see …