Life Sciences Commons^™

Using Camera Stations To Investigate Occupancy Probability And Habitat Associations Of Island Spotted Skunks (Spilogale Gracilis Amphiala) On Santa Rosa And Santa Cruz Islands, Desirae Thomaier

Master's Theses

The California Channel Islands are unique in that two islands (Santa Rosa and Santa Cruz) support two endemic mesocarnivores, the island fox (Urocyon littoralis) and the island spotted skunk (Spilogale gracilis amphiala). Following the near-extinction of island foxes in the early 2000s, island spotted skunk populations increased significantly. Now that foxes have recovered, skunk captures have precipitously declined - possibly due to interspecific competition with foxes who have a similar diet but a larger body size and wider temporal niche. It has been proposed that skunks selectively use canyon bottoms and drainages with high vegetation cover and that these areas …

Protein Digestibility Impacted By Bacillus Coagulans, Pasteurization And Protein Source In Ready-To-Drink Protein Beverages, Samantha L. Bass, Samantha L. Bass

Master's Theses

The goal of this study was to assess how to maximize the digestibility of protein in protein beverages utilizing various protein sources, differing temperature/storage combinations, and the addition of probiotics (Bacillus coagulans). The protein sources used included 3 milk proteins and 2 plant protein sources (micellar casein, milk protein concentrate, reduced-calcium milk protein concentrate, pea protein isolate, and soy protein isolate). Soy protein isolate was chosen because it is readily available and commonly used as a plant protein alternative. Pea protein isolate was chosen because it is a non-allergen, and similarly, it is readily available. Milk protein concentrates …

Optimizing Biofungicide Use In Managing Winegrape Powdery Mildew On The Central Coast Of California, Edgar G. Godoy Monterroso

Master's Theses

Grapevine powdery mildew caused by the fungal pathogen Erysiphe necator is a persistent threat to wine grape production in California. While synthetic fungicides remain the primary management strategy, increasing concerns over pesticide resistance, environmental impact, and regulatory restrictions have driven interest in biofungicides as a sustainable alternative. However, their efficacy is inconsistent, requiring research into optimized application strategies, integration with synthetic fungicides, and their effects on disease suppression, grape yield, and fruit quality.

This two-year study (2023–2024) was conducted at two vineyard sites, Trestle Vineyard in the San Luis Obispo Coast AVA and a commercial vineyard in the Arroyo Grande …

Treating Ground Water For Agricultural And Individual Consumption, Gabby Miller, Bronson Scott, Michael Clawson

Biomedical Engineering

This project, conducted in collaboration with Cal Poly seniors and Da Vinci High School students, aims to develop a solar-powered groundwater purification system. This system is designed to provide clean water for agricultural use and individual consumption, addressing the growing concerns of groundwater contamination and accessibility needs. The final prototype integrates a gravity filtration system combined with chemical purification, utilizing aluminum sulfate and chlorine to remove particulates, bacteria, and organic compounds.

The research phase involved an extensive review of groundwater contamination issues, existing filtration technologies, and market analysis. Key design constraints included cost-effectiveness, ease of maintenance, and minimal energy consumption, …

Quantifying The Mechanisms Behind Carbon Sequestration And Soil Health Following Compost Application In A Rangeland Chronosequence, Grace Damaschino

Master's Theses

Compost application to rangelands has the potential to sequester carbon (C) and add essential plant nutrients to the soil. The California Department of Food and Agriculture's (CDFA) Healthy Soils Project (HSP) provides financial support to farmers and landowners to implement innovative practices that promote soil health. The CDFA is currently recommending compost application rates of 6-10 tons per acre; however, previous research suggests that degraded soils may require a larger dose of compost to overcome limitations, therefore this recommendation might not meet soil health or soil carbon sequestration objectives. This study examines the compost rate effects on soil health, while …

3d Printed Microfluidic Fabrication Methodology, Characterization, Mechanical Design, And Applications In Electrostatic Artificial Muscles And Benthic Microbial Fuel Cells, Terak B. Hornik

Master's Theses

The fabrication of microfluidic devices often requires specialized methods. The development of these methods requires careful characterization and understanding of the processes involved. Using primarily PolyJet 3D printing technology, microfluidics offers a wide scope of applications such as microfluidic benthic microbial fuel cells (MBMFCs) and electrostatic artificial muscles. MBMFCs benefit from the confinement of the microbes resulting in close proximity between the electrode and the organisms. Using a modular design called the Sponge, assembly and upscaling is possible. Electrostatic artificial muscles benefit from a microfluidic approach due to the non-linearity of electrostatic attraction creating disproportionate benefits when miniaturized. When designed …

"Deep Learning For Microscope Image Denoising", Nasreen Buhn, Sriya Adunur, Guy Hagen, Jonathan Ventura

College of Engineering Summer Undergraduate Research Program

In order to avoid damaging live cells, optical microscope imaging must be conducted under low-excitation light intensity and/or short exposure times, resulting in low signal-to-noise ratios (SNR). Deep learning methods offer an effective solution for removing microscope noise, utilizing algorithms that are able to reconstruct finer features in low SNR images. This research explores the denoising capability of several deep learning methods based on PSNR and SSIM. Tested methods include traditional approaches (BMED), supervised learning (CARE and Restormer), and unsupervised methods (Noise2Fast, N2V, SSD-Unsupervised, and SASSID). The Restormer model, which employs an encoder-decoder transformer architecture and progressive learning, stood out …

Seal Counting On Our Plages (S.C.O.O.P.), Kaanan Kharwa

Master's Theses

The Vertebrate Integrative Physiology (VIP) lab monitors the population of northern elephant seals at the largest mainland breeding colony, located at Piedras Blancas (San Simeon, CA). As the population expands, more human-seal interactions and conflicts over land use occur. The VIP lab's work informs California State Parks and helps with the management of the rookery. Currently, members of the VIP lab fly a drone over the beaches, capture multiple images, and manually count the seals, which takes around 14 to 21 hours of analysis per survey. Machine learning methods such as Convolutional Neural Networks (CNN) and Region-based Convolutional Neural Networks …

Barriers To Implementation Of California Vegetation Treatment Program Projects, Jacquelyn Morales

Master of Science in Environmental Sciences and Management Projects

To better defend wild lands within California's state responsibility areas against wildfire, progress toward efficient fuels management is vital. Created in effort to increase pace and scale of forest fuels reduction, the California Vegetation Treatment Program (CalVTP) serves as method to expedite fire fuel reduction, fuel break development, and ecological restoration projects by streamlining environmental regulatory approval. To better assess the progress of fuels management projects across the state, this professional project aims to look at barriers faced by project proponents when using the CalVTP. A questionnaire was emailed to the project proponents behind the 81 unique CalVTP projects in-progress. …

Of Geese And Grass: Investigating Impacts Of Brant Grazing On Eeelgrass, Dakota L. Osborne

Master's Theses

Grazing pressure is important in structuring plant communities, particularly in aquatic environments. Eelgrass (Zostera marina), a keystone species in estuaries across North America, is experiencing widespread population declines. As a keystone species, eelgrass provides numerous important functions, including: 1) water filtration, 2) sediment stabilization, 3) providing refuge and nursery habitat for numerous species, and 4) carbon sequestration. Benefits which could be lost if eelgrass beds continue to diminish. California has experienced some of the greatest eelgrass declines, and Morro Bay, CA experienced a 96% decrease in eelgrass from 2007 to 2017. Most studies to date have examined bottom-up …

Development Of Extruded Puffed Snack Utilizing Carrot Pomace, Bean Flour, And Rice Starch As Primary Ingredients, Julia Zoe

Master's Theses

Every year, the carrot industry worldwide wastes up to 50% of carrots as raw material due to processing (Šeregelj et al., 2020). When carrots are juiced, the remaining fibrous pulp is often discarded as carrot pomace. In order to redirect this food waste, the first objective of this research was to optimize formulations for an extruded puffed snack that is most similar to commercially available puffed snacks, utilizing baby lima bean flour and carrot pomace

Hardness (kg), cross-sectional expansion ratio, bulk density (g/cm³), moisture content (%), water activity, specific mechanical energy (kJ/kg), carotenoid concentration (ppm), and total dietary …

Survey Of Soilborne Pathogens Infecting Strawberry In Oxnard, California & Effect Of Planting Date And Chill Treatment On Yield Of Strawberry, Samantha Z. Simard

Master's Theses

Survey to quantify the frequency of the five main pathogens (Macrophomina phaseolina, Fusarium oxysporum f. sp. fragariae races 1 and 2, Verticillium dahliae, and Phytophthora spp.) infecting strawberry in the Oxnard growing district of California. M. phaseolina was the most prevalent pathogen detected in diseased plants in the Oxnard growing district, present in 67.6% of samples positive for at least one pathogen. F. oxysporum f. sp. fragariae race 1 (35.3%), V. dahlia (8.8%), F. oxysporum f. sp. fragariae race 2 (5.9%) and Phytophthora spp. (2.9%) followed at lower prevalence. Associations were found between fewer drip irrigation lines …

Gps Accuracy Of Smartphones For Crowdsourcing Research, Derron L. Dike

Master of Science in Environmental Sciences and Management Projects

ABSTRACT GPS Accuracy of Smartphones for Crowdsourcing Research Derron Dike Utilizing crowdsourcing with smartphones as a method for field data collection can contribute an array of data for scientific studies and land management applications. This study used crowdsourcing to examine smartphone built-in GPS performance for providing location-specific information with potential forestry applications. The usability of smartphones for GPS data collection in forestry studies or other practical applications is dependent on the level of accuracy required for those applications. A Smartphone Accuracy Trial was designed to test the GPS accuracy performance of current smartphones. Participants used the ArcGIS Field Maps application …

Education And Land Management On The Pacific Crest Trail Phase 4, Ben Sherman, Emma Perry, Cade Cappello, Hattie Cahill, Anna Macklyn, Aidan Tull, Tristian Xu, Augrey Gregg

Baker/Koob Endowments Awarded Projects

This research is a continuation of research collected on the Pacific Crest Trail (PCT). The purpose of this study was to investigate land ethics and education, Leave No Trace principles, and hiker experiences on the PCT. From July 2023-January 2024, interdisciplinary students collected qualitative and quantitative data through interviews and surveys with hikers on the trail. Past phases of this project have determined that this research is necessary, but due to Covid, the team has only been able to gather data remotely via online surveys and interviews prior to this study. The Baker Koob grant funded travel and research equipment …

Cultivating Agriculture - Cultivando Agricultura: Content Creator, Sophia L. Garcia

Agricultural Education: Graduate Internship Reports

Agriculture is rooted in culture, yet we rarely discuss the people who drive the industry today. Many voices have yet to share their stories of influencing or making a difference in the agriculture industry. As a result, the idea of a podcast arose as a free, convenient, and accessible way for everyone to hear and communicate stories. During the project, my co-host, Daniela Ramos, and I will work together to address this issue. To diversify and separate the workload, we will each host three episodes individually. As part of my role, I will also be responsible for marketing, including creating …

The Effect Of Probiotic Supplementation On Serum Metabolite Concentrations In Young Holstein Heifers, Emily G. Meissner

Master's Theses

The objective of this trial was to evaluate the effect of a multi-strain probiotic on serum concentrations of selected metabolites in young calves. Holstein heifers (n = 112) from a commercial dairy were enrolled within 48 h of birth and placed into individual hutches. Calves were weaned at 60 d of age and remained in their hutches until 90 d of age, when they were moved to group housing. Calves were placed into 2 treatment groups: 1) control and 2) B. subtilis, B. lichenformis, L. animalis, and P. freudenreichii probiotic (Bovamine Dairy Plus, Chr. Hansen, Milwaukee, WI). Control calves received …

Aged 539 - Graduate Internship, Braidyn R. Cossa

Agricultural Education: Graduate Internship Reports

This report reflects on the Cal Poly Rodeo Program's alignment with the National Standards for Sport Coaches, showcasing a comprehensive approach to athlete development across multiple critical domains. Emphasizing a well-defined coaching philosophy, the program promotes sportsmanship, ethical behavior, and academic success. Personalized coaching addresses the developmental stages of athletes, ensuring a balance between their academic and athletic roles. The program employs effective teaching methods, clear communication, and constructive feedback to foster a positive environment and build strong coach-athlete relationships based on mutual respect and trust.

The coaches possess deep knowledge of rodeo skills and strategies, utilizing psychological techniques to …

Implementation And Efficiency Of Electric Motors In Lygus Bug Vacuums For Strawberries, Adam J. Rantz

Master's Theses

Further automation of the agricultural industry and an increase in organic fruit production are needed to address labor shortage and increased demand. The move to an autonomous bug vacuum is easier if the equipment is electric, and not powered by a tractor’s PTO (power take-off) shaft. The main objective of this project was to design a set of lygus bug vacuums to go on a robot that meets industry standards while using electric power instead of hydraulic. The frame of the vacuum was designed using the industry standard. While hydraulic systems have been a very effective power source for vacuums …

Deciphering The Contribution Of Microglia To Neurodegeneration In Friedreich's Ataxia, Sydney N. Gillette

Master's Theses

Friedreich's ataxia (FRDA) is the most prevalent inherited ataxia, affecting one in every 50,000 individuals in the United States. This hereditary condition is caused by an abnormal GAA trinucleotide repeat expansion within the first intron of the frataxin gene resulting in decreased levels of the frataxin protein (FXN). Insufficient cellular frataxin levels results in iron accumulation, increased reactive oxygen species production and mitochondrial dysfunction. Tissues most heavily impacted are those most dependent on oxidative phosphorylation as an energy source and include the nervous system and muscle tissue. This is evident in the clinical phenotype which includes muscle weakness, ataxia, neurodegeneration …

Identification Of Downy Mildew Resistance In Wild Arugula And Evaluation Of Downy Mildew Causing Pathogens And Management Practices, Reilly B. Blair

Master's Theses

Downy mildew has been a potentially devastating constraint to crop production within the Brassicaceae family worldwide (CABI, 2022) and on the Central Coast of California (Smukler, et al, 2008; Koike, et al. 2007). Downy mildew is a foliar disease, caused by the pathogens in the Hyaloperonospora genus on plants within the Brassicaceae family (Goker, et al, 2009). Downy mildew is a disease that is common in region of the world with Coastal climates that provide humidity and moderate temperatures (Sarahan, et al, 2017). Crops within the Brassicaceae family maintain a predominant role in agricultural systems worldwide (Cartea, et al, 2011) …

Red Raspberries Attenuate Blood Glucose Spike Of A High Glycemic Load Breakfast Cereal, Dante J. Martin

Master's Theses

Blood glucose (BG) spikes increase chronic disease risk in those with and without diabetes. The fiber and phytonutrient content of fruit, such as raspberries has the potential to attenuate the BG spike of a high glycemic load (HGL) meal. We examined the effect of red raspberries (RRs) on the BG spike when added to a HGL meal. In this randomized cross-over trial, 16 university students were given breakfast cereal and almond milk (control), the control breakfast with 140g added RRs, and the control breakfast with sugar equivalent to the sugar content of the RRs (50% sugar increase from control). Meals …

Emerging Diseases, Abiotic Disorders, And Macrophomina Root Rot Management Of California Strawberry, Cooper Calvin

Master's Theses

Strawberry is an economically important crop in California, with an estimated value of $2.68 billion in 2023. In California strawberry production, mitigation of low plant health and yield often focuses on major soilborne pathogens, while the contribution of minor pathogens and abiotic disorders to production shortcomings are often overlooked. The objectives of the first project in this thesis are to determine the pathogenicity of multiple minor pathogens and quantify other biotic and abiotic factors that can reduce plant health such as viruses and soil salinity. Two pathogens of the black root rot complex, Pythium spp. and Rhizoctonia spp. as well …

Design And Test The Effectiveness Of Interpretive Signs Using Eye Tracking And Biometric Data, Hadara Gordon, Wendy Miyazaki

Baker/Koob Endowments Awarded Projects

Recreational trails on forested lands should satisfy the needs of recreationists, safeguard important habitats, and maintain the natural environment (Kortenkamp et al., 2021). Appropriate management is critical because of the increasing number of visitors. Signs are a cost-effective method to reduce the negative impacts on visitors and enhance visitor experiences (Brown et al., 2010). This research aimed to investigate how visitors pay attention to signs, view the trail surrounded by trees and behave in a natural space.

Automated Tree Mortality Detection Using Ubiquitously Available Public Data, Michael T. Huggins

Master's Theses

Understanding the dynamic interplay between fire severity, topography, and tree mortality, is crucial for predicting future forest dynamics and enhancing resilience against climate change-induced wildfire regimes. This thesis develops a multi-sensor approach for automated estimation of tree mortality, then applies it to examine trends in tree mortality over a six-year period across a fire affected study site in the Trinity River basin in Northern California. The Random Forest model uses publicly available USGS 3D Elevation Program Lidar (3DEP) and NAIP imagery as inputs and is likely to be easily adaptable to other landscapes. The model had a Receiver Operating Characteristic …

Effects Of Anthropogenic Noise On The Provisioning Behavior Of Western Bluebirds And Artificial Light At Night On Nestling Development, Kerstin H. Ozkan

Master's Theses

Sensory environments are rapidly changing due to increased human activity in urban and non-urban areas alike. For instance, background sounds can interfere with parent-offspring communication and mask cues reflective of predation risk, resulting in elevated vigilance at the cost of provisioning. In chapter 1, we studied nestling provisioning behavior among Western Bluebirds (Sialia mexicana) in response to short-term (1 hr) and long-term (continuous exposure throughout nesting period) noise exposure. Provisioning rates were lower at nests exposed to short-term experimental traffic noise compared to exposure to ambient background sounds. Trial order strongly influenced provisioning behavior, with the decline in …

Coralai: Emergent Ecosystems Of Neural Cellular Automata, Aidan A. Barbieux, Aidan A. Barbieux

Master's Theses

Artificial intelligence has traditionally been approached through centralized architectures and optimization of specific metrics on large datasets. However, the frontiers of fields spanning cognitive science, biology, physics, and computer science suggest that intelligence is better understood as a multi-scale, decentralized, emergent phenomenon. As such, scaling up approaches that mirror the natural world may be one of the next big advances in AI. This thesis presents Coralai, a framework for efficiently simulating the emergence of diverse artificial life ecosystems integrated with modular physics. The key innovations of Coralai include: 1) Hosting diverse Neural Cellular Automata organisms in the same simulation that …

Effect Of Chloropicrin And Metam Sodium On Soil Nitrogen Dynamics In California Strawberry Production, Colin Timothy Koubek

Plant Sciences

California strawberries (Fragaria x ananassa Duch.) bring in ~3 billion yearly for the state's economy. Strawberry growers use fumigants like chloropicrin and metam sodium to manage a variety of soilborne diseases in conventional production. The yield-boosting effects of fumigation on strawberries are often attributed to disease management, but the ‘fertilizer effect’ from mineralization may play an equally significant role. This experiment conducted in a grower field sought to find out the influence that fumigation had on the mineralization of soil organic nitrogen and if preplant fertilizer application affected early season nitrogen (N). There were six treatments: control, control with controlled …

Evaluating Corporate Marine Plastic Pollution Reduction Pledges: Achieving Plastic Reductions Or Contributing To Greenwashing, Ansar Z. Mohammed

Master of Science in Environmental Sciences and Management Projects

This paper examines major corporations' commitments and performance in reducing virgin plastic production, aligning corporate initiatives with tangible outcomes. It draws on sustainability governance literature and analyzes plastic reduction strategies by industry leaders such as SC Johnson, PepsiCo, Coca-Cola, Borealis, Nestlé, and Unilever. By synthesizing corporate reports and scholarly literature, the research provides an understanding of corporate plastic reduction within the framework of Sustainable Development Goal 14 (SDG 14), which focuses on marine conservation. The study evaluates the alignment of corporate commitments with SDG 14, identifying genuine environmental stewardship and potential greenwashing. It employs three methodologies: transparency, accountability, and science-based …

Can Functional Traits Predict Turnover Of Alpine Plant Species In Yosemite National Park?, Brooke L. Wallasch

Biological Sciences

Alpine species are a useful tool for predicting the effects of climate change on lowland species. A growing body of research has begun assessing the question of how alpine species will change in response to changing climate. In the study of alpine plant species, some researchers have begun exploring the idea that functional traits could be predictive of turnover in alpine plant communities. In this study, I used a species’-level approach to assess the ability of six functional traits (vegetative height, generative height, leaf thickness, leaf area, leaf dry weight, specific leaf area) to predict species turnover in alpine plants …

Exploring Representation In Microbiology Introductory Courses Can Encourage A More Inclusive And Inspiring Environment For Students And Instructors, Jill A. Mikucki, Elizabeth Fozo

Feminist Pedagogy

Microbiology has a relatively brief history where significant discoveries are often linked with major events in human history - from disease outbreak to industrialization to climate change. The founders of key microbiological principles span across continents, genders, ethnicities, and socioeconomic status. However, the portrait described in many introductory textbooks center around a lone, typically white male scientist. Such narratives not only are misleading regarding the development of key principles in microbiology but can also reinforce inappropriate stereotypes as to whom belongs in microbiology. In our introductory microbiology course, we designed group work for Zoom break-out rooms to help engage students …