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California Polytechnic State University, San Luis Obispo

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Full-Text Articles in Life Sciences

Feeling Out Your Food: A Histological Analysis Of The Whisker System In Seals, Cameron Cooper Jul 2020

Feeling Out Your Food: A Histological Analysis Of The Whisker System In Seals, Cameron Cooper

Master's Theses

The vibrissal (whisker) system is present in nearly all mammals and is especially important in deep-diving mammals. Pinnipeds (seals, sea lions, and walruses) have highly sensitive whiskers that are used to follow hydrodynamic trails created by their swimming prey. Each pinniped vibrissa is surrounded by a tripartite blood sinus system composed of an upper cavernous sinus (UCS), a ring sinus (RS), and a lower cavernous sinus (LCS). The UCS has been hypothesized to play a thermoregulatory role, insulating temperature sensitive mechanoreceptors located within the vibrissal follicle. The aim of this study was to examine this hypothesis by measuring and comparing …


Associations Between Variations In Breast Anatomy And Early Breastfeeding Challenges, Alison K. Ventura, Brittany Lore, Olga Mireles Jun 2020

Associations Between Variations In Breast Anatomy And Early Breastfeeding Challenges, Alison K. Ventura, Brittany Lore, Olga Mireles

Kinesiology and Public Health

Background: Mothers with anatomic variability (e.g., shorter, wider nipples; denser areolas) may experience breastfeeding challenges disproportionately.

Research aim: To examine whether variations in breast anatomy are associated with risk for early breastfeeding challenges.

Methods: Participants included mothers < 6 weeks postpartum. Nipple base width, nipple length, and areolar density were measured on the right and left breast separately. Experiences with early breastfeeding challenges were determined through a combination of maternal report and clinical assessment.

Results: Participants (N = 119) had an average nipple diameter of 23.4 (SD = 3.0) mm for left nipples and 23.5 (SD = 3.0) mm for right nipples (range = 10–34 mm). Average nipple length was 8.5 (SD = 3.2) mm for left breasts and 9.1 (SD = 3.2) mm for right breasts (range = 5–20 mm); 35% of participants had dense areolas …


Saturday Night Live Club Lamb Sale, Tyler P. Wilkerson Jun 2020

Saturday Night Live Club Lamb Sale, Tyler P. Wilkerson

Agricultural Education and Communication

“Competition is said to be the backbone of the stock show industry,” (Davis, 1998). A statement spoken in a 1998 thesis paper on the junior livestock industry still holds water in today’s world. Youth livestock exhibition, especially in California, continues to grow in the number of youth participants and the number of livestock entries at shows (Switzer, 2019).

It is the responsibility of livestock breeders to breed, raise, and sell their livestock to maximize the opportunity for youth to acquire livestock to show. In his research, Wendt discusses the capabilities of purchasing livestock through online platforms. He speaks to the …


Becoming Agricultured: Creating An Agriculture Literacy Podcast For Consumers, Madalyn C. Souza Jun 2020

Becoming Agricultured: Creating An Agriculture Literacy Podcast For Consumers, Madalyn C. Souza

Agricultural Education and Communication

Today’s consumers want to become more aware of where their food comes from. There is a need and a desire to bridge the gap between consumers and production agriculture. One way to reach consumers is through audio podcasts. Podcasts are digital audio recordings that are streamed online. The podcast Becoming Agricultured will be a platform to show the relationship between agriculture and the environment, food, fiber and energy, animals, lifestyle, the economy and technology. The podcast is aimed to tell agriculture's story through factual research and personal accounts of those involved in different sectors of the agriculture industry and cultivate …


Hericium Erinaceus: Erinacine A, Ventura Villanueva Jun 2020

Hericium Erinaceus: Erinacine A, Ventura Villanueva

Agricultural Education and Communication

Hericium erinaceus (Lion's Mane)

Erinacines - component found in Lion's Mane

A variety of mushroom that has been grabbing the attention of different pharmaceutical companies is the Hericium Erinaceus commonly known as Lion’s Mane. Lion’s Mane also known as, “Sheep’s head, Bear’s head, Bearded tooth carpophore, and Yamabushitmnnake” (Lion’s Mane (Hericium erinaceus)). Lion’s Mane in nature is found growing off the dry bark of trees (Kuo, 2016). Nowadays there are many products in the market that sell Lion’s Mane mushroom powder as a superfood. Lion’s Mane mushroom is known to have the component erinacines. Erinacines as of …


Increasing Groundwater Recharge Activity In The San Joaquin Valley Of California, Jacob Willhite, Jacob Willhite Jun 2020

Increasing Groundwater Recharge Activity In The San Joaquin Valley Of California, Jacob Willhite, Jacob Willhite

Agricultural Education and Communication

Over the past several decades, groundwater has become a primary source of water used for agriculture in California. Surface water available for agricultural use has depleted due to declining rain totals and reallocation to environmental purposes. As a result, groundwater overdraft has become a severe challenge, especially in the San Joaquin Valley of California. This excessive overdraft causes a plethora of issues, one of the most serious being land subsidence (Faunt, Sneed, Traum, & Brandt, 2016). Studies suggest some areas of the San Joaquin Valley have experienced more than a 28-foot drop in the land level since the 1970’s (Alley …


Public Relations Of Cal Poly Avocado Rootstock Trial, Angel Daniel Carrillo Jun 2020

Public Relations Of Cal Poly Avocado Rootstock Trial, Angel Daniel Carrillo

Agricultural Education and Communication

Avocados have been one of California’s specialty crops for quite some time now. The industry has been motivating growers to implement the best management practices to ensure stable and increased production as much as they possibly can. California is the leading state in the country for avocado production. In 2019, California alone produced 109 tons of avocados (US Department of Agriculture, 2020). Unfortunately, root rot has made it a challenge to do so.

Root rot is a disease derived from the Phytophthora cinnamomi pathogen. This is an issue for avocado production worldwide. In efforts to find a method of decreasing …


Towards Equitable Communication: An Exploratory Study To Guide Knowledge Translation In Kinesiology, Akuekegbe Y. Uwadiale, Jafra D. Thomas Jun 2020

Towards Equitable Communication: An Exploratory Study To Guide Knowledge Translation In Kinesiology, Akuekegbe Y. Uwadiale, Jafra D. Thomas

Kinesiology and Public Health

Resources at or below the 8th grade reading level is key if behavioral resources are to support end-user health literacy, a strong predictor of prevention-oriented health behaviors and health risk. To understand how readability issues effect physical activity resources, we performed a systematic review of the kinesiology literature. A sample of 18 original studies was obtained (M publication date=2010, SD=7.45), which on average reported public resources exceeded the 8th grade reading level by at least two grades. Thematic analysis resulted in 14 general ways material readability could be easily improved. Project findings will be discussed and next steps presented.


Graduate Internship Report - Central High School, Yesenia A. Ramirez Jun 2020

Graduate Internship Report - Central High School, Yesenia A. Ramirez

Agricultural Education: Graduate Internship Reports

This internship report includes reflections and supporting documentation required in meeting the twelve quality criteria for secondary-level Agriculture Education Programs in the State of California. The quality criteria are used to evaluate the quality of an agriculture program and determine its eligibility for Agriculture Incentive Grant funds. The supporting documentation includes information used to receive state and local funding, outline the goals and objectives of the program, along with an overview of Central West, the agriculture program, and the community.


Graduate Internship Report: Golden West High School, Amber R. Nagel Jun 2020

Graduate Internship Report: Golden West High School, Amber R. Nagel

Agricultural Education: Graduate Internship Reports

This internship and project report includes documentation required in meeting the quality criteria for secondary-level programs of instruction in agriculture. The documents are concurrently used for the Agriculture Incentive Grant review process at Golden West High School conducted by representatives of the California Department of Education. The supporting material includes information to receive state and local funding, outline the goals and objectives of the program, along with an overview of Golden West High School, the agriculture program and the community.


Analysis Of Biofilm Remediation Capacity For Octenyl Succinic Anhydride (Osa), A Bioactive Food Starch Modifier Compound, Matthew R. Borglin Jun 2020

Analysis Of Biofilm Remediation Capacity For Octenyl Succinic Anhydride (Osa), A Bioactive Food Starch Modifier Compound, Matthew R. Borglin

Master's Theses

Matthew R. Borglin

This thesis demonstrates efficacy of Octenyl Succinic Anhydride (OSA), as a biofilm sanitizer. Biofilms allow bacteria to adhere to solid surfaces with the use of excreted polymeric compounds. For example, surfaces found in food production or processing facilities such as the interior of a raw milk holding tank, are some of the most susceptible to biofilm contamination. When present, biofilms can cause a variety of negative effects, which include; reduction of product shelf life, corrosion, and outbreaks of foodborne illnesses. The close association of biofilms with the majority of foodborne illness cases led the US Environmental Protection …


A Floristic Study Of The Cal Poly Swanton Pacific Ranch And A New Combination In Sanicula Crassicaulis (Apiaceae), Sanicula Crassicaulis Var. Nudicaulis, Reed J. Kenny Jun 2020

A Floristic Study Of The Cal Poly Swanton Pacific Ranch And A New Combination In Sanicula Crassicaulis (Apiaceae), Sanicula Crassicaulis Var. Nudicaulis, Reed J. Kenny

Master's Theses

Globally we are experiencing a biodiversity crisis and potentially a sixth mass extinction event. Plant specimens are one of the best, most concrete records of biodiversity that we can create. Despite this, the rate of plant collecting has declined steeply since World War II. Now more than ever, plant collections are vital, both for the purpose of quantifying the plant biodiversity in an area and for discovering previously unrecognized diversity.

In Chapter 1, we conducted a floristic survey of the Cal Poly Swanton Pacific Ranch. The Swanton Pacific Ranch (SPR) is located north of the small town of Davenport California, …


Biography Of Leann L Birch, Phd, 25 June 1946 – 26 May 2019, Stephani Anzman-Frasca, Kirsten K. Davison, Jennifer O. Fisher, Lori A. Francis, Susan L. Johnson, Justin A. Lavner, Ian M. Paul, Alison K. Ventura, Jennifer S. Savage Jun 2020

Biography Of Leann L Birch, Phd, 25 June 1946 – 26 May 2019, Stephani Anzman-Frasca, Kirsten K. Davison, Jennifer O. Fisher, Lori A. Francis, Susan L. Johnson, Justin A. Lavner, Ian M. Paul, Alison K. Ventura, Jennifer S. Savage

Kinesiology and Public Health

On 26 May, 2019, the nutrition community lost a visionary ambassador, trusted advisor, and cherished mentor. Leann Birch was a pioneer in bringing a developmental psychology perspective to the study of children's nutrition as a means to respond to real-world questions raised by parents. Leann Elsie Traub was born in Owosso, Michigan 25 June, 1946. She grew up primarily in Southern California and received a bachelor's degree in psychology from California State University, Long Beach, in 1971. She completed her graduate studies at the University of Michigan where she received a master's degree in 1973 and a doctorate in 1975, …


Cal Poly Pier Master Plan, Troy A. Lawson Jun 2020

Cal Poly Pier Master Plan, Troy A. Lawson

Master's Theses

The Cal Poly Pier (Pier) Master/Facility Plan (FP) document provides the vision of the future for the Pier, a marine science research facility. The Plan facilitates project development and management of the Pier while meeting university and department research goals. Specifically, the FP document establishes goals and strategies to direct long-term development of the Pier, streamlines agency approval and permit requirements, provides context for pier management, and assists the permitting process for future development as it relates to regulatory permits and programmatic growth on the Cal Poly Pier to help meet goals of the Center for Coastal Marine Sciences (CCMS). …


Engineering A Grapevine Pruning Training Tool For Use In Educational And Professional Environments, Catherine Anne Thornbury Jun 2020

Engineering A Grapevine Pruning Training Tool For Use In Educational And Professional Environments, Catherine Anne Thornbury

Electrical Engineering

This report documents the design of a field-safe and portable tool that helps field hands identify how many wine grape nodes to prune per cane. This practice allows for a more bountiful harvest. The device indicates to the worker whether to leave one, two, or three nodes on a cane. The device is attachable to a set of industry-standard shears to allow for efficient and cost-effective pruning.

The device add-on can withstand a typical 8-hour workday and is able to be used in bright sun, clouds, and rain. Additionally, the device is able to differentiate between small, medium, and large …


Portable Fluorescence Illuminator, Katherine Hui, Emran Baryal, Emmett Lambert Jun 2020

Portable Fluorescence Illuminator, Katherine Hui, Emran Baryal, Emmett Lambert

Mechanical Engineering

This Final Design Review (FDR) document outlines the senior design project implemented by a team of three mechanical engineering students at California Polytechnic State University, San Luis Obispo for a professor in the biochemistry and chemistry department, Dr. Javin Oza. The project involved designing and manufacturing a portable ultraviolet fluorescence illuminator that will indicate the fluorescent glow of biomaterials for viewing purposes. The goal was to create a portable product out of recyclable material that can be used in field applications and increase accessibility to affordable science equipment. The initial sponsor and user interviews, technical background research, product research, and …


Analysis And Optimization Of Colorimetric Nanosensors For Rapid Detection Of Microbes In Water, Ruby A. Lang Jun 2020

Analysis And Optimization Of Colorimetric Nanosensors For Rapid Detection Of Microbes In Water, Ruby A. Lang

Master's Theses

Access to safe water is a basic human right recognized by the United Nations General Assembly in 2010 (WHO, 2020). However, a least 2.2 billion people globally still are without safely managed water services meaning they use a drinking water source that can be contaminated with faeces (WHO, 2020). With such a pressing global health issue, it is clear that improvement to water systems is important and required in the Agenda 2030 Sustainable Development Goals (SDGs). However, to improve water systems and prove they are safe water sources, water quality testing must occur. A solution to this issue is the …


Creating A Livestock Show From The Beginning For The Benefit Of Youth In The Agriculture Industry And Continuing The Tradition Of Showing, Chloé Fowler, Matthew Durian Jun 2020

Creating A Livestock Show From The Beginning For The Benefit Of Youth In The Agriculture Industry And Continuing The Tradition Of Showing, Chloé Fowler, Matthew Durian

Agricultural Education and Communication

Youth throughout the country have been shown to benefit through organizations focused on leadership and real-world tangible skills. Organizations such as the National FFA Organization and 4-H Youth Development are key organizations for this growth. Livestock shows are another layer to these organizations and youth development. “Livestock shows provide interactions through different environments that expose participants to different cultures,” (Davis, 1998).

For these reasons it is important to provide more opportunities to the next generation of agriculture. There are currently 28 different livestock jackpots within the state of California throughout one year, (CJLA, 2020). Due to the large geographic landscape …


The Influence Of The Sensory Environment On Avian Reproductive Success And Human Well-Being, Danielle Marie Ferraro Jun 2020

The Influence Of The Sensory Environment On Avian Reproductive Success And Human Well-Being, Danielle Marie Ferraro

Master's Theses

Sensory pollutants such as anthropogenic noise and night lighting now expose much of the world to evolutionarily novel sound and night lighting conditions, which can have detrimental effects on humans and wildlife. In my first chapter, we exposed wild Western Bluebird (Sialia mexicana) nestlings to noise, light, and combination (i.e., noise and light) treatments. Nests exposed to noise and light together experienced less predation than control and light-exposed nests, and noise-exposed nests experienced less predation than control nests, yet overall nest success was only higher in noise-exposed nests compared to light-exposed nests. Although exposure to light decreased nestling body condition …


Maturation And Culture Media Effects On In Vitro Bovine Embryo Developmental Competence, Ciara M. Helland Jun 2020

Maturation And Culture Media Effects On In Vitro Bovine Embryo Developmental Competence, Ciara M. Helland

Master's Theses

In vitro produced bovine embryos are critical to the cattle industry. However, these embryos have altered morphology, epigenetics, and metabolism when compared to their in vivo counterparts. The aim of this thesis was to alter maturation and culture media to improve the developmental competence of in vitro bovine embryos. This thesis is comprised of three experiments and one proof of concept study. Each experiment followed the same general layout: oocyte aspiration from Jersey or Holstein ovaries, oocyte maturation for 24 hours, fertilization with bull semen for 24 hours, then embryo culture for 7-8 days in 38.5 °C in 5% O …


Attractiveness Of English Thyme (Thymus Vulgaris L.) To Arthropod Natural Enemies And Its Suitability As A Dual Use Resource, Kristina K. Van Wert Jun 2020

Attractiveness Of English Thyme (Thymus Vulgaris L.) To Arthropod Natural Enemies And Its Suitability As A Dual Use Resource, Kristina K. Van Wert

Master's Theses

Current agroecosystem management practices have a negative effect on natural enemies and their ability to control insect pests. Conservation biological control through the addition of flowering resources can manage food resources for natural enemies. These floral resources can also provide multiple ecosystem services. Study goals were to determine if perennial Thymus vulgaris L. was attractive to natural enemies and if so, could it be a dual use resource encouraging pest management and providing harvestable product. In 2018 plots in three locations were used to examine the effect of habitat throughout the growing season on the attractiveness of T. vulgaris. …


Reduction Of Fat Content In Processed Meats Using Hot-Boning And Cold-Batter Mincing Technology, Morgan P. Wonderly Jun 2020

Reduction Of Fat Content In Processed Meats Using Hot-Boning And Cold-Batter Mincing Technology, Morgan P. Wonderly

Master's Theses

Processed meats have received negative publicity due to high fat contents that have been linked to adverse effects on human health. Fat is an essential ingredient in many processed meat products, so reducing this all while maintaining the desired characteristics of the product is a challenge. The purpose of this study was to generate low-fat meat products using a combination of hot-boning/crust-freeze-air-chilling (HB-CFAC) and cold-batter mincing (CBM) technologies. Pork hams were subjected to HB-CFAC or chill-boning/crust-freeze-air-chilling (CB-CFAC) prior to 3 min pre-mincing and 6 min mincing for control gels with back-fat addition or low-fat gels with water addition instead of …


Pismo Clams (Tivela Stultorum) In Califorina: Population Status, Habitat Associations, Reproduction, And Growth, Alexandria R. Marquardt May 2020

Pismo Clams (Tivela Stultorum) In Califorina: Population Status, Habitat Associations, Reproduction, And Growth, Alexandria R. Marquardt

Master's Theses

Marine shellfish play a vital role in intertidal ecosystems and coastal communities, but many of these fisheries are small-scale and lack the necessary monitoring to ensure long-term sustainability. Effective management often requires information on key demographic parameters, such as population status, reproduction and growth. Pismo clams (Tivela stultorum) are a culturally important and iconic species in California, which supported a thriving commercial and recreational fishery throughout much of the 1900’s. However, Pismo clam populations have declined statewide in recent decades and are attributed to human harvest and predation by California sea otters (Enhydra lutris); However, no …


Seasonal And Interannual Variability Of Phytoplankton Abundance And Community Composition On The Central Coast Of California, Alex Barth, Ryan K. Walter, Ian C. Robbins, Alexis Pasulka Mar 2020

Seasonal And Interannual Variability Of Phytoplankton Abundance And Community Composition On The Central Coast Of California, Alex Barth, Ryan K. Walter, Ian C. Robbins, Alexis Pasulka

Physics

Variations in the abundance and composition of phytoplankton greatly impact ecosystem structure and function. Within the California Current System (CCS), phytoplankton community structure is tightly coupled to seasonal variability in wind-driven coastal upwelling, a process that drives changes in coastal water temperatures and nutrient concentrations. Based on approximately a decade (2008-2018) of weekly phytoplankton measurements, this study provides the first characterization of the seasonal and interannual variability of phytoplankton abundance and composition in San Luis Obispo (SLO) Bay, an understudied region within the CCS. Overall, the seasonality of phytoplankton in SLO Bay mirrored that of the larger CCS; diatoms dominated …


Inactivation Of Foodborne Pathogens During Cider Fermentation, In A Cider Model System And Commerical Cider, Kathryn K. Yamada Mar 2020

Inactivation Of Foodborne Pathogens During Cider Fermentation, In A Cider Model System And Commerical Cider, Kathryn K. Yamada

Master's Theses

Hard cider is an alcoholic drink made from fermented crushed fruit, typically apples. The popularity of this fermented alcoholic beverage has been on the rise within the last decade. Historically, hard cider has been deemed safe due to the presence of ethanol and the low pH. Although there is lack of scientific evidence to prove that hard cider will and can be safe from foodborne pathogens. Escherichia coli O157:H7, Salmonella spp., and Listeria monocytogenes are three predominate foodborne bacterial pathogens of concern in the food and beverage industry. Escherichia coli O157:H7 in particular has been associated with fresh produce and …


Thermal Ecology Of The Federally Endangered Blunt-Nosed Leopard Lizard, Kathleen N. Ivey Mar 2020

Thermal Ecology Of The Federally Endangered Blunt-Nosed Leopard Lizard, Kathleen N. Ivey

Master's Theses

Recognizing how climate change will impact populations can aid in making decisions about approaches for conservation of endangered species. The Blunt-nosed Leopard Lizard (Gambelia sila) is a federally endangered species that, despite protection, remains in extremely arid, hot areas and may be at risk of extirpation due to climate change. We collected data on the field-active body temperatures, preferred body temperatures, and upper thermal tolerance of G. sila. We then described available thermal habitat using biophysical models, which allowed us to (1) describe patterns in lizard body temperatures, microhabitat temperatures, and lizard microhabitat use, (2) quantify the …


Valorization Of Carrot Processing Waste, Alexandra M. Duval Mar 2020

Valorization Of Carrot Processing Waste, Alexandra M. Duval

Master's Theses

Commercial carrot processors produce up to 175,000 tons of carrot waste annually. Carrot Mash (CM) is the term referring to the waste by-product of peeled baby carrot processing. Transportation of carrot processing waste is expensive due to its high- water content (approx. 83-95%). High in bioactive compounds (carotenoids) and dietary fibers, it is expected that its conversion into a value-added by-product is of interest to the carrot processing industry. Hemicellulose-rich plant materials have proven to be a source of oligosaccharides, which are known for their beneficial prebiotic activity. The objectives of this research were to: 1) determine the effect of …


Fruit, Flower, And Thorn: A Phenological Study Of 'Wonderful' Pomegranate, Kylie M. Mendonca Mar 2020

Fruit, Flower, And Thorn: A Phenological Study Of 'Wonderful' Pomegranate, Kylie M. Mendonca

Master's Theses

‘Wonderful’ pomegranate flowering and fruiting habits are not well understood. Characterizing any relationship between flowering habit and fruit size and quality could lead to effective cultural practices for producing high-value fruit. Five mature ‘Wonderful’ pomegranate trees were monitored every 5-14 days throughout the 2015 growing season. Newly emerged flowers were tagged and characterized with respect to sex (determined by ovary shape and style length at anthesis), clustering habit, age of wood on which floral buds were borne, and direction of wood growth. More than 1,100 hermaphroditic flowers were identified and ovary/fruit diameter was recorded throughout the season. In addition to …


Patterns In Size Distribution And Catch Of Rockfish (Sebastes Spp.) In Fisheries-Independent And Fisheries- Dependent Hook-And-Line Surveys On The Central Coast Of California, Rose Elizabeth Dodgen Mar 2020

Patterns In Size Distribution And Catch Of Rockfish (Sebastes Spp.) In Fisheries-Independent And Fisheries- Dependent Hook-And-Line Surveys On The Central Coast Of California, Rose Elizabeth Dodgen

Master's Theses

Stock assessments are statistical models which characterize the state of a population of fish. Data for stock assessment models of West Coast nearshore groundfish come largely from fisheries-dependent sources. Incorporating fisheries-independent data would increase data availability. A potential source of fisheries-independent data which is comparable to existing fisheries-dependent data is the California Collaborative Fisheries Research Program (CCFRP), a Marine Protected Area (MPA) monitoring study. We are interested in understanding the context in which CCFRP could be implemented into assessments of nearshore groundfish, specifically rockfish. To investigate this, we used management-relevant metrics to examine three questions concerning the implementation of CCFRP …


Development And Validation Of The Maternal Distraction Questionnaire, Alison K. Ventura, Megan Hupp, Shawnee Alvarez Gutierrez, Rebeca Almeida Feb 2020

Development And Validation Of The Maternal Distraction Questionnaire, Alison K. Ventura, Megan Hupp, Shawnee Alvarez Gutierrez, Rebeca Almeida

Kinesiology and Public Health

This paper describes the development of a self-report measure of mothers’ engagement in technological activities during mother-infant interactions. In Study 1, mothers (n = 332; infants: 3.8 ± 1.4 months) completed the Maternal Distraction Questionnaire (MDQ) and related questionnaires. Factor analysis revealed two distinct subscales representing engagement with technological distractors and perceived distraction. Subscales correlated with relevant measures of feeding styles, attachment, and infant eating behaviors and temperament. In Study 2, mothers (n = 24; infants: 3.8 ± 1.8 months) completed the MDQ and kept feeding activity diaries. Significant correlations between MDQ subscales and diary data were noted. …