Life Sciences Commons^™

Occupancy Modeling Of Western Monarch Thanksgiving Counts: Negative Impacts Of Incomplete Resurveys And Uneven Sampling Efforts, Keegan M. Mclean

Biological Sciences

Western monarch butterflies (sp. Danaus plexippus) are undergoing a severe decline that rivals those occurring among insects across the globe. Despite the estimation of population abundance, growth rates, and extinction probabilities, no analyses have investigated spatiotemporal patterns of decline in the western monarch population. I performed occupancy modeling of Western Monarch Thanksgiving Count (WMTC) data. The data was constrained spatially and temporally, with sites grouped into occupancy bins by latitude and year. Occupancy probabilities (psi) were estimated for each intersection of a latitude and time bin and detection probabilities (p) were estimated for each time bin. Psi increased slightly …

Lotus: A Web-Based Computational Tool For The Preliminary Investigation Of A Novel Mst Method Utilizing A Library Of 16s Rrna Bacteroides Otus, Ginger Dewitte

Master's Theses

Microbial Source Tracking (MST) is a field of study that attempts to identify the source of fecal contamination in waterways in order to assist with development of remediation strategies. Biologists at Cal Poly Center for Applications in Biotechnology (CAB) are developing a new MST method using microbes from the genus Bacteroides. Bacteroides species are host-specific microorganisms that can theoretically be used to trace back to a single host species. After fecal samples are collected, biologists use Next-Generation Sequencing (NGS) techniques to obtain only the genetic sequences of microorganisms belonging to the phylum Bacteroidetes. Investigators hypothesize that similar sequences belong …

Sipping Sustainably: A Case Study On Daou Family Estates, Nicole Grant

Experience Industry Management

In today’s era, year 2021, there is a high emphasis placed on sustainability in a business. Companies are adopting practices that encompass environmental, social, and economic sustainability. The purpose of this study was to examine the three pillars of sustainability at DAOU Family Estates. DAOU Vineyards is a winery located in Paso Robles, California, that is known for its production of Cabernet Sauvignon. Data were gathered through a case study guide that outlined the current sustainability efforts put into place at DAOU Family Estates. A case study guide was assembled by the researcher to evaluate the different categories of sustainability …

Natural And Experimental Noise Affects Acoustic Communication In Songbirds, Veronica Arlene Reed

Master's Theses

Background noise is ubiquitous and can impair acoustic communication and influence signaling behavior in animals. Despite evidence demonstrating myriad effects of anthropogenic noise on animal communication, precisely how natural background noise influences communication and behavior remains unclear. Yet, natural sources of background noise, such as rushing rivers or crashing ocean surf, share similar power spectra to sources of anthropogenic noise and can occur at high amplitudes, potentially masking acoustic signals.

To investigate the effects of water-generated noise on songbird behavior, we experimentally broadcast landscape-level playbacks of ocean surf and river noise in coastal California, USA, and riparian habitat in Idaho, …

Associations Between Maternal Technology Use, Perceptions Of Infant Temperament, And Indicators Of Mother-To-Infant Attachment Quality, Shawnee Alvarez Guitierrez, Alison K. Ventura

Kinesiology and Public Health

Background: Previous research suggests parents' use of technological devices, such as TV and mobile devices, within family contexts may decrease the quality of parent-child interactions. During early infancy, mothers report engaging with technological devices during infant feeding and care interactions, however, few studies have explored potential associations between maternal technology use and the quality of mother-to-infant attachment.

Aim: To examine associations between maternal technology use during mother-infant interactions and indicators of mother-to-infant attachment during early infancy.

Study design: Cross-sectional survey.

Methods: Mothers (n = 332) of infants aged 2 to 6 months were recruited via MTurk, a crowdsourcing platform, …

Seasonal Controls On Nearshore Hypoxia In A Small Coastal Embayment, Stephen Alexander Huie

Physics

Dissolved oxygen (DO) is an important biogeochemical factor that strongly influences nearshore coastal ecosystems. Low DO (hypoxic) events can cause physiological stressful environments for ecological and economically important species, potentially leading to mass mortalities. In order to better assess drivers of coastal hypoxia, we collected data from monthly cruises on the inner shelf and nearshore moorings inside and outside a small coastal embayment (San Luis Obispo Bay on the Central California Coast) across the full upwelling season (March to August). During the late spring and early summer, we found that the nearshore near-bottom temperature-DO (T-DO) relationship aligned with the shelf …

Early Wildfire Detection With Line Sensors, Virginia Yan

Master's Theses

Over the last few years, wildfires have become more devastating to communities as the fires are inevitably destructive to many homes, businesses, and ecosystems. Frequent wildfires also pose a significant threat to power grids and nearby residents as they can damage transmission lines and other electrical equipment, which in turn can cause major power shutdowns. Especially in western U.S., severe drought conditions and weather variability cause residents to become more vulnerable to wildfire disasters as their safety is threatened. We are incompetent to control the wildfires effectively despite existing advanced technologies. Hence, an algorithm based on energy conservation and heat …

The Effects Of Wort Oxygenation Scenarios On Fermentation Performance, Volatile Flavor Compound Development, And Flavor Stability In High Gravity Brewing, Ben Jabson

Master's Theses

High gravity (HG) brewing has become the most used strategy for maximizing fermenter productivity in commercial brewing. While HG brewing has many benefits, the additional stress placed on the yeast due to the higher concentration of fermentable sugars in the wort can negatively impact fermentation performance and flavor compound formation. A proper dissolved oxygen (DO) level is vital to guarantee adequate yeast performance during HG fermentations. Dissolved oxygen is vital to yeast viability throughout the fermentation process, as yeast requires oxygen to synthesize vital cell membrane components needed for continued anaerobic growth and cell division. Previous research has demonstrated the …

The Response Of Marine Synechococcus To A Landscape Of Environmental Stressors: A Proteomic Exploration, Dana E. Michels

Master's Theses

In the field of marine microbial ecology, many questions remain unanswered with regards to the physiological trade-offs made by phytoplankton to maximize growth (e.g., nutrient acquisition) and minimize loss (e.g., predation defenses). These tradeoffs, which occur at the cellular level, have wide reaching impacts on food web dynamics and global biogeochemical cycles. In the first chapter, we explored the use of a non-canonical amino acid (NCAA) technique, bioorthogonal non-canonical amino-acid tagging (BONCAT), in phytoplankton model systems. This technique has potential to work well in natural systems by enabling isolation of only newly synthesized proteins during an incubation period with the …

Effect Of Prenatal Lifestyle Intervention On Maternal Postpartum Weight Retention And Child Body Massindex Z-Score At 36 Months, Suzanne Phelan, Chantelle N. Hart, Elissa Jelalian, Karen Muñoz-Christian, Noemi Alarcon, Angelica Mchugh, Alison K. Ventura, Rena R. Wing

Kinesiology and Public Health

Background/Objectives We previously reported results from a randomized trial showing that a behavioral intervention during pregnancy reduced excess gestational weight gain but did not impact maternal weight at 12 months. We now examine the longer-term effects of this prenatal intervention on maternal postpartum weight retention and toddler body-mass-index z scores (BMIz) over 36 months.

Subjects/Methods Pregnant women (N = 264; 13.7 weeks’ gestation; 41.6% Hispanic) with overweight or obesity were randomized into usual care or prenatal intervention. Anthropometric assessments in mothers and toddlers occurred at baseline, 35 weeks’ gestation and after delivery at 6, 12, 18, 24, and 36 …

The Future Of Organic Insect Pest Management: Be A Better Entomologist Or Pay For Someone Who Is, David H. Headrick

Horticulture and Crop Science

Insect pest management in certified organic production systems presents considerable challenges for growers. The Federal National Organic Program (NOP) guidelines list acceptable tactics, but their effective use requires a considerable knowledgebase in entomology. The range of tactics allowed by the NOP are viewed as limiting by many growers and there are important elements missing from the list such as pest monitoring and identification. Educational programs must consider utilizing instructional methods and additional means of outreach that introduce new pest management tactics that are individualized, regionally appropriate and emphasize grower adoption and collaboration with local professionals. This review describes the challenges …

Comparative Effects Of Chemical And Physical Sunscreen On Fertilization Of Purple Sea Urchins (Strongylocentrotus Purpuratus), Marilla Lippert, Maurice Codespoti Goodman, Nikki Adams

Biological Sciences

Organic compound-based “chemical” sunscreens dominate the commercial sunscreen market, but recent research has revealed the ingredients of these products are detrimental to the health of marine organisms. This revelation has led to increased popularity of mineral-based “physical” sunscreens, primarily containing zinc-oxide (ZnO), as environmentally safe alternatives. While they are marketed as environmentally safe, these claims are largely untested, and it is important to consider potential effects of ZnO-based sunscreens on the development of marine organisms. Though Zn is a necessary micronutrient in the ocean, excess Zn is released into marine environments from anthropogenic sources has negative effects on marine life. …

Suspicion Encoded: Women Of Color And Biometric Technology In The United States, Lilith A. Saylor

sprinkle: an undergraduate journal of feminist and queer studies

This paper explores the commodification of privacy through biometric technology in the United States. It examines the impact of this technology on poor women of color (WOC), arguing that poor WOC face intersectional discrimination based on the convergence of sex, race, and class in their identities. I highlight the unique and powerful intrusion of biometric technology into the lives of poor WOC, and argue that the connection between data and the physical body created through biometric data has formed an environment in which the state wields unrestricted control in all spheres over the privacy of poor WOC.

Effect Of Simulated Storage And Distribution On Listeria Innocua Growth In Non-Traditional Salad Ingredients, Emma L. Sandquist

Master's Theses

The fresh-cut produce industry has seen expansive growth in recent years, to meet consumer demand ready-to-eat (RTE) salads have included the use of non-traditional ingredients. Uncommon ingredients include beet greens, kale, broccoli stalk, and Brussels sprouts, since these ingredients have not historically been consumed raw, potential food safety issues should be reassessed. Current processing technologies include produce washes that can reduce microbial levels but do not eradicate all populations. The lack of a kill step in produce processing emphasizes the need to minimize pathogen contamination during production and growth during a product’s shelf life. Listeria monocytogenes, a leading cause …

Estimating Food Waste Due To Food Safety Recalls And Investigating Ways To Minimize Negative Impacts, Mykayla Latronica

Master's Theses

For years the issue of food waste has been recognized and quantified; however, food safety issues often go unrecognized as a source of food waste. One objective of this research is to estimate quantities and monetary value of fruits and vegetables implicated in food safety recalls, and thus wasted. Using publicly available data we identified all recalls involving vegetable or fruit commodities contaminated with Listeria monocytogenes, pathogenic E. coli, or Salmonella during 2015-2018. When quantities were provided, monetary value of recalled product was calculated using USDA ERS 2016 average retail prices. Although data limitations only allowed analysis of …

Strawberry Powdery Mildew Caused By Podosphaera Aphanis: Fungicide Resistance And Host Plant Resistance, Michael G. Palmer

Master's Theses

Strawberry powdery mildew, caused by Podosphaera aphanis, affects leaves, fruit, and runners of strawberry plants. Infected leaves have reduced photosynthetic capability and infected fruit become unmarketable. Both of these factors translate to economic loss for the grower and therefore merit taking measures to control the disease. One objective of this study was to evaluate the resistance developed in populations of strawberry powdery mildew to chemical control measures. A fungicide assay was developed to evaluate the efficacy of six treatments (penthiopyrad, quinoxyfen, myclobutanil, trifloxystrobin, cyflufenamid, fluopyram + trifloxystrobin) for control of the disease. Nineteen isolates of strawberry powdery mildew were …

Study Of Plants Used Against Infections By California Native American Tribes, Maria J. Rojas

Master's Theses

The objectives of this research were to evaluate the antibacterial activity and to determine the chemical composition of a list of medicinal plants used by Native Americans in California. Artemisia californica, Mimulus aurantiacus, Equisetum telmateia, Equisetum hyemale, and Marah fabacea were selected from a list of plants reported as having been used for ailments related to infections by tribes located in California. The extracts obtained through steam distillation from E. telmateia, E. hyemale and M. fabacea were assayed for in vitro antibacterial activity against 16 Gram-negative and 6 Gram-positive bacteria using disk diffusion assays and measuring the diameters of inhibition …

Potential Overfeeding Among Formula Fed Special Supplemental Nutrition Program For Women, Infants And Children Participants And Associated Factors, Christopher E. Anderson, Catherine E. Martinez, Alison K. Ventura, Shannon E. Whaley

Kinesiology and Public Health

Background: The Special Supplemental Nutrition Program for Women, Infants and Children (WIC) provides enough formula to meet the known nutritional needs of infants up to 6 months of age whose mothers report not breastfeeding, but many mothers report WIC providing insufficient formula, indicating potential overfeeding.

Objective: To estimate the prevalence of potential overfeeding among formula-feeding WIC participants and identify associated factors.

Methods: Potential overfeeding was identified among participants of the longitudinal Infant and Toddler Feeding Practices Study-2 (ITFPS-2) receiving the fully formula feeding WIC infant package at 1 month of age (n = 1235, weighted n = 197 079). Associations …

Improving Sustainable Fertilizer Practices For Pomegranate By Leaf Nutrient Concentration Evaluation And Fertilizer Trials, Minh Le

Master's Theses

Fruit tree leaf nutrient concentrations are commonly used to determine fertilizer rates, but information is limited regarding nutrient requirements, seasonal N uptake and removal rates, and soil N dynamics for pomegranate. Relationships between fertilizer rates, leaf nutrient concentrations, fruit yield and quality were examined in five mature, commercial California ‘Wonderful’ pomegranate orchards. Site 1 was observed for two growing seasons (2018-2019) and sites 2-5 were observed for one season (2018 or 2019). In 2018, 150, 300, or 450 g N/tree was applied at sites 1-3 in a single application at early fruit development or in two equal applications at early …

Eelgrass (Zostera Marina) Population Decline In Morro Bay, Ca: A Meta-Analysis Of Herbicide Application In San Luis Obispo County And Morro Bay Watershed, Tyler King Sinnott

Master's Theses

The endemic eelgrass (Zostera marina) community of Morro Bay Estuary, located on the central coast of California, has experienced an estimated decline of 95% in occupied area (reduction of 344 acres to 20 acres) from 2008 to 2017 for reasons that are not yet definitively clear. One possible driver of degradation that has yet to be investigated is the role of herbicides from agricultural fields in the watershed that feeds into the estuary. Thus, the primary research goal of this project was to better understand temporal and spatial trends of herbicide use within the context of San Luis …

Positive Parenting Approaches And Their Association With Child Eating And Weight: A Narrative Review From Infancy To Adolescence, Katherine N. Balantekin, Stephanie Anzman-Frasca, Lori A. Francis, Alison K. Ventura, Jennifer O. Fisher, Susan L. Johnson

Kinesiology and Public Health

Parents play a critical role in the development of children's eating behaviours and weight status, serving as providers, models and regulators of the food environment. Many research reviews have focused on the robust body of evidence on coercive control in feeding: how parenting practices such as restriction and pressure to eat increase children's risk for developing undesirable eating behaviours and unhealthy weight outcomes. Fewer reviews adopt a strengths-based perspective focusing on the ways that parents can actively support the development of healthy eating behaviours and weight trajectories. Emerging research on such positive parenting styles and practices offers solutions beyond the …

A Naturalistic Ethic Supporting A Vegan Diet, Michael Morris

Between the Species

Nutritional evidence suggests that a vegan diet is the most adaptive one for humans. An ethical principle based on following our biological nature (naturalistic ethic) could therefore provide additional support for a vegan diet. However, some argue that humans in the natural world could not eat a vegan diet, since it relies on supplements, particularly vitamin B12. This leads to the conclusion that humans are naturally omnivores, and therefore our natural diet should include small amounts of animal products.

Three approaches to this conclusion are discussed. The first rejects a naturalistic ethic in favour of normative principles based on animal …

Transgenerational Plasticity Causes Differences In Uv-Tolerance Of Intertidal And Subtidal Populations Of The Purple Sea Urchin, Strongylocentrotus Purpuratus, Yareli Alvarez, Nikki L. Adams

Master's Theses

Planktonic larvae of marine organisms are increasingly being exposed and required to respond to a changing physical environment. Adult sea urchins occupy both intertidal and subtidal waters and broadcast spawn gametes into the water column to contend with variable physical conditions. To answer how populations of invertebrates residing at different depths adequately prepare their offspring to cope with different levels of ultraviolet radiation (UVR), we collected adult purple sea urchins, Strongylocentrotus purpuratus, from four sites (two intertidal and two subtidal (~15 m deep)) on the central coast of CA to compare UV tolerance in offspring. Our measurements of UVA …

Pressure Driven Desalination Utilizing Nanomaterials, Fangyou Xie

Master's Theses

Nanomaterials such as graphene oxide and carbon nanotubes, have demonstrated excellent properties for membrane desalination, including decrease of maintenance, increase of flux rate, simple solution casting, and impressive chemical inertness. Here, two projects are studied to investigate nanocarbon based membrane desalination. The first project is to prepare hybrid membranes with amyloid fibrils intercalated with graphene oxide sheets. The addition of protein amyloid fibrils expands the interlayer spacing between graphene oxide nanosheets and introduces additional functional groups in the diffusion pathways, resulting in increase of flux rate and rejection rate for the organic dyes. Amyloid fibrils also provide structural assistance to …

Sensory Stressors Impact Species Responses Across Local And Continental Scales, Ashley A. Wilson

Master's Theses

Pervasive growth in industrialization and advances in technology now exposes much of the world to anthropogenic night light and noise (ANLN), which pose a global environmental challenge in terrestrial environments. An estimated one-tenth of the planet’s land area experiences artificial light at night — and that rises to 23% if skyglow is included. Moreover, anthropogenic noise is associated with urban development and transportation networks, as the ecological impact of roads alone is estimated to affect one-fifth of the total land cover of the United States and is increasing in space and intensity. Existing research involving impacts of light or noise …

Maleic Anhydride Compatibilized Peach Waste As Filler In Polypropylene And High Density Polyethylene Biocomposites, Caralyn Wong, Stephanie Jung, Joongmin Shin, Ajay Kathuria

Master's Theses

It is estimated that roughly 103, 515 tons of peach waste is produced annually in the US. The majority of the waste is disposed of in landfills, which contributes to climate change as they release 93 million metric tons of CO₂ equivalent. Peach waste principally consists of remaining stone and seed after flesh removal. The agro-waste includes both cellulose and lignin, which can be utilized as a filler in plastic packaging to reduce carbon footprints and material cost. The objectives of this research are (1) to develop peach flour (PF)-filled biocomposites with a polyolefin matrix using maleic anhydride-g-high density …

Artificial Night Lighting And Anthropogenic Noise Alter Animal Activity, Body Condition, Species Richness, And Community Structure, Joshua Willems

Master's Theses

Sensory pollution from artificial night-lighting and anthropogenic noise have increased at a dramatic rate over the last several decades. Alterations to the sensory environment have been found to affect wildlife in a wide variety of ways including behavioral changes, physiological responses, changes in species interactions, and altered community structure. Increased levels of light and noise pollution can originate from many sources including roads, energy development and infrastructure, and urbanization. Even remote or protected areas are not immune to the effects of increased sensory disturbances with 63 percent of protected areas within the United States found to have been exposed to …

Lipid Quantification And Cryopreservation Of In Vitro Produced Jersey Cattle Embryos, Katherine A. Rhodes-Long

Master's Theses

Cryopreservation of in vivo derived Jersey bovine embryos have resulted in a 10% lower pregnancy rate compared to other dairy breeds. Poor embryo survival after cryopreservation has been partially attributed to the high lipid content of Jersey embryos. In vitro-produced (IVP) bovine embryos have darker cytoplasm than their in vivo-derived counterparts because of higher lipid accumulation. High lipid accumulation is associated with impaired embryo quality. Forskolin is an adenylate cyclase activator that regulates cAMP levels in cells and has been shown to induce lipolysis in IVP embryos. L-carnitine is required for transport of fatty acids from the intermembrane space of …

Quantifying The Ecological Drivers And Impacts Of Parrotfish Predation On Caribbean Corals Communities, Hannah Sima Rempel

Master's Theses

Parrotfishes (Scarinae) are dominant Caribbean herbivores that play an important role in reducing coral-algae competition by grazing algae; yet some species are also occasional coral predators (corallivores) and thereby can have direct negative impacts on coral growth and survivorship. There is concern that parrotfish corallivory may contribute to substantial long-term declines in targeted coral species, particularly in areas with a high biomass of parrotfishes and low cover of corals. However, the capacity of target coral species to heal from parrotfish predation and the ecological drivers of corallivory are poorly understood.

In Chapter 1, we examined the patterns of coral healing …

Feeling Out Your Food: A Histological Analysis Of The Whisker System In Seals, Cameron Cooper

Master's Theses

The vibrissal (whisker) system is present in nearly all mammals and is especially important in deep-diving mammals. Pinnipeds (seals, sea lions, and walruses) have highly sensitive whiskers that are used to follow hydrodynamic trails created by their swimming prey. Each pinniped vibrissa is surrounded by a tripartite blood sinus system composed of an upper cavernous sinus (UCS), a ring sinus (RS), and a lower cavernous sinus (LCS). The UCS has been hypothesized to play a thermoregulatory role, insulating temperature sensitive mechanoreceptors located within the vibrissal follicle. The aim of this study was to examine this hypothesis by measuring and comparing …