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Articles 391 - 420 of 2421

Full-Text Articles in Life Sciences

Performance Of Black Soldier Fly Frass Fertiliser On Maize (Zea Mays L.) Growth, Yield, Nutritional Quality, And Economic Returns, C. M. Tanga, D. Beesigamukama, M. Kassie, P. J. Egonyu, Changeh J. Ghemoh, Kiatoko Nkoba, S. Subramanian, A. O. Anyega, S. Ekesi Jan 2022

Performance Of Black Soldier Fly Frass Fertiliser On Maize (Zea Mays L.) Growth, Yield, Nutritional Quality, And Economic Returns, C. M. Tanga, D. Beesigamukama, M. Kassie, P. J. Egonyu, Changeh J. Ghemoh, Kiatoko Nkoba, S. Subramanian, A. O. Anyega, S. Ekesi

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Although the black soldier fly frass fertiliser (BSFFF) is globally recognised as a promising and potential high-quality organic fertiliser, there is inadequate information on its impact on the growth performance and nutritional status of maize. Furthermore, no information exist on their combination with mineral fertiliser (NPK) as well as economic impact on maize production. This study evaluated the comparative impact of BSFFF, NPK and commercial organic fertiliser (Evergrow®) on growth, nitrogen use efficiency, yield, nutritional quality, and profitability of maize under greenhouse conditions. The treatments included: (1) sole application of BSFFF, conventional compost of brewers’ spent grain (BSG), Evergrow, and …

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Nitrogen Use Efficiency Trends For Sustainable Crop Productivity In Lake Victoria Basin: Smallholder Farmers' Perspectives On Nitrogen Cycling, Winnie Ntinyari, Joseph Gweyi-Onyango, Mekonnen Giweta, Benson Mochoge, James Mutegi, Generose Nziguheba, Cargele Masso Jan 2022

Nitrogen Use Efficiency Trends For Sustainable Crop Productivity In Lake Victoria Basin: Smallholder Farmers' Perspectives On Nitrogen Cycling, Winnie Ntinyari, Joseph Gweyi-Onyango, Mekonnen Giweta, Benson Mochoge, James Mutegi, Generose Nziguheba, Cargele Masso

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Nitrogen Use Efficiency (NUE) is one of the established metrics for benchmarking management of Nitrogen (N) in various systems. Numerous approaches to calculateNUEexist, making it difficult to compare the performances of systems depending on the methodology used. This study adopted the conceptualized framework by European Union Nitrogen Expert Panel (EUNEP) to calculateNUE values for cereal crops to determine future trends for the first time in the Lake Victoria region. Data were collected through in-person interviews among maize and rice smallholder farmers within the Lake Victoria region. Atotal of 293 observations were recorded. Collected data on yield andNfertilizer were used to …

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Distribution, Abundance And Natural Enemies Of The Invasive Tomato Leafminer, Tuta Absoluta (Meyrick) In Kenya, G. Kinyanjui, F. M. Khamis, F. L.O. Ombura, E. U. Kenya, S. Ekesi, S. A. Mohamed Dec 2021

Distribution, Abundance And Natural Enemies Of The Invasive Tomato Leafminer, Tuta Absoluta (Meyrick) In Kenya, G. Kinyanjui, F. M. Khamis, F. L.O. Ombura, E. U. Kenya, S. Ekesi, S. A. Mohamed

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Tuta absoluta (Meyrick) has become a serious menace to sustainable production of tomato in Kenya. A survey was conducted between April 2015 and June 2016 to determine its distribution, abundance, infestation, and damage levels on tomato, and associated natural enemies. Trap counts of T. absoluta moths were recorded in all surveyed 29 counties, which indicated its nationwide distribution irrespective of altitude. Tuta absoluta was present in both open fields and greenhouses. The highest moth/trap/day was 115.38 ± 15.90. Highest leaf infestation was 92.22% and the highest number of mines and larvae per leaf were 3.71 ± 0.28 and 2.16 ± …

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Scorpionfish (Scorpaena Porcus) Neurocranium, Erin Porter, Cairone Reft, David Kerstetter Dec 2021

Scorpionfish (Scorpaena Porcus) Neurocranium, Erin Porter, Cairone Reft, David Kerstetter

All Scans: Kerstetter Fisheries and Avian Ecology 3D Scan Series

Neurocranium preparation of a Scorpionfish (Scorpaena porcus) by Erin Porter and Cairone Reft for the Biology of Fishes course at Nova Southeastern University taught by Dr. Kerstetter.

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Kingfish Neurocranium, Kaden R. Mackey, David Kerstetter Dec 2021

Kingfish Neurocranium, Kaden R. Mackey, David Kerstetter

All Scans: Kerstetter Fisheries and Avian Ecology 3D Scan Series

This is the skull of a kingfish with the nasal bone included.

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Grey Triggerfish (Balistes Capriscus) Neurocranium, Madelynn Sampson, Hailey Levine, David W. Kerstetter Dec 2021

Grey Triggerfish (Balistes Capriscus) Neurocranium, Madelynn Sampson, Hailey Levine, David W. Kerstetter

All Scans: Kerstetter Fisheries and Avian Ecology 3D Scan Series

Grey Triggerfish (Balistes capriscus) neurocranium preparation by Madelynn Sampson and Hailey LeVine.

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Mahi Mahi (Coryphaena Hippurus) Neurocranium, Alexia J. Hilber, Caileigh Craddock, Kiara Belanger, David W. Kerstetter Dec 2021

Mahi Mahi (Coryphaena Hippurus) Neurocranium, Alexia J. Hilber, Caileigh Craddock, Kiara Belanger, David W. Kerstetter

All Scans: Kerstetter Fisheries and Avian Ecology 3D Scan Series

Neurocranium from Mahi Mahi (Coryphaena hippurus), collected in 3/2021 by Katerina Sawickji

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The Nutritional Profiles Of Five Important Edible Insect Species From West Africa—An Analytical And Literature Synthesis, Jacob P. Anankware, Benjamin J. Roberts, Xavier Cheseto, Isaac Osuga, Vincent Savolainen, C. M. Collins Dec 2021

The Nutritional Profiles Of Five Important Edible Insect Species From West Africa—An Analytical And Literature Synthesis, Jacob P. Anankware, Benjamin J. Roberts, Xavier Cheseto, Isaac Osuga, Vincent Savolainen, C. M. Collins

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Background: Undernutrition is a prevalent, serious, and growing concern, particularly in developing countries. Entomophagy—the human consumption of edible insects, is a historical and culturally established practice in many regions. Increasing consumption of nutritious insect meal is a possible combative strategy and can promote sustainable food security. However, the nutritional literature frequently lacks consensus, with interspecific differences in the nutrient content of edible insects generally being poorly resolved. Aims and methods: Here we present full proximate and fatty acid profiles for five edible insect species of socio-economic importance in West Africa: Hermetia illucens (black soldier fly), Musca domestica (house fly), Rhynchophorus …

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Centropomus Undecimalis, Common Snook Neurocranium, Victoria Rose Kelley, Savanna Duda, David W. Kerstetter Dec 2021

Centropomus Undecimalis, Common Snook Neurocranium, Victoria Rose Kelley, Savanna Duda, David W. Kerstetter

All Scans: Kerstetter Fisheries and Avian Ecology 3D Scan Series

Neurocranium from Centropomus undecimalis (Common Snook), collected from NSU Oceanographic Campus Boat Basin on 24/03/2021 by Savanna Duda.

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[Red Grouper (Epinephelus Morio)] Neurocranium, Laurel Gallaudet, Robert Spekis Dec 2021

[Red Grouper (Epinephelus Morio)] Neurocranium, Laurel Gallaudet, Robert Spekis

All Scans: Kerstetter Fisheries and Avian Ecology 3D Scan Series

Neurocranium from 14.1 cm total length (TL) Red Grouper (Epinephelus morio), collected from Miami on 13/10/21 by Laurel Gallaudet.

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Red Grouper (Epinephelus Morio) Neurocranium, Katja C. Kramers, Morgan A. Pfeiffer, David W. Kerstetter Dec 2021

Red Grouper (Epinephelus Morio) Neurocranium, Katja C. Kramers, Morgan A. Pfeiffer, David W. Kerstetter

All Scans: Kerstetter Fisheries and Avian Ecology 3D Scan Series

Neurocranium From 10 cm total length Red Grouper (Epinephelus morio), collected from Whole Foods Supermarket on 10 October 2021 by Katja Kramers and Morgan Pfeiffer

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Insect Flower-Visitors Of African Oil Palm Elaeis Guineensis At Different Sites And Distances From Natural Vegetation In Uganda, James Peter Egonyu, Stephen Erisa Sisye, John Baguma, Milton Otema, Gabriel Ddamulira Dec 2021

Insect Flower-Visitors Of African Oil Palm Elaeis Guineensis At Different Sites And Distances From Natural Vegetation In Uganda, James Peter Egonyu, Stephen Erisa Sisye, John Baguma, Milton Otema, Gabriel Ddamulira

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This survey aimed at generating information to guide improvement of oil palm fruit-set in Uganda by testing the hypothesis that diversity and abundance of oil palm insect flower-visitors were influenced by sampling site, distance from natural vegetation and flower sex. Sampling was done on male and female oil palm flowers from (i) five oil palm experimental fields in different districts and (ii) oil palm plantations on Bugala Island on Lake Victoria. The plantations on Bugala Island were subdivided into near (0–15 m) and far (100–200 m) from natural vegetation. We recorded 162,677 insects in 23 morpho-species, dominated by weevils in …

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King Mackerel (Scomberomorus Cavalla), Aubrey L. Anthony, Erin Keeley, David Kerstetter Dec 2021

King Mackerel (Scomberomorus Cavalla), Aubrey L. Anthony, Erin Keeley, David Kerstetter

All Scans: Kerstetter Fisheries and Avian Ecology 3D Scan Series

Neurocranium from [length] cm total length (TL) King Mackerel (Scomberomorus Cavalla), collected from Bravo Market on 11/16/2021 by Aubrey Anthony

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The Pest Kill Rate Of Thirteen Natural Enemies As Aggregate Evaluation Criterion Of Their Biological Control Potential Of Tuta Absoluta, Joop C. Van Lenteren, Alberto Lanzoni, Lia Hemerik, Vanda H.P. Bueno, Johanna G. Bajonero Cuervo, Antonio Biondi, Giovanni Burgio, Francisco J. Calvo, Peter W. De Jong, Silvia N. López, M. Gabriela Luna, Flavio C. Montes, Eliana L. Nieves, Pascal Osa Aigbedion-Atalor, Maria B. Riquelme Virgala, Norma E. Sánchez, Alberto Urbaneja Dec 2021

The Pest Kill Rate Of Thirteen Natural Enemies As Aggregate Evaluation Criterion Of Their Biological Control Potential Of Tuta Absoluta, Joop C. Van Lenteren, Alberto Lanzoni, Lia Hemerik, Vanda H.P. Bueno, Johanna G. Bajonero Cuervo, Antonio Biondi, Giovanni Burgio, Francisco J. Calvo, Peter W. De Jong, Silvia N. López, M. Gabriela Luna, Flavio C. Montes, Eliana L. Nieves, Pascal Osa Aigbedion-Atalor, Maria B. Riquelme Virgala, Norma E. Sánchez, Alberto Urbaneja

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Ecologists study how populations are regulated, while scientists studying biological pest control apply population regulation processes to reduce numbers of harmful organisms: an organism (a natural enemy) is used to reduce the population density of another organism (a pest). Finding an effective biological control agent among the tens to hundreds of natural enemies of a pest is a daunting task. Evaluation criteria help in a first selection to remove clearly ineffective or risky species from the list of candidates. Next, we propose to use an aggregate evaluation criterion, the pest kill rate, to compare the pest population reduction capacity of …

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Semiochemical Signatures Associated With Differential Attraction Of Anopheles Gambiae To Human Feet, Maurice O. Omolo, Isaiah O. Ndiege, Ahmed Hassanali Dec 2021

Semiochemical Signatures Associated With Differential Attraction Of Anopheles Gambiae To Human Feet, Maurice O. Omolo, Isaiah O. Ndiege, Ahmed Hassanali

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Background: Several human-produced volatiles have been reported to mediate the host-seeking process under laboratory conditions, yet no effective lure or repellent has been developed for field application. Previously, we found a gradation of the attractiveness of foot odors of different malaria free individuals to Anopheles gambiae sensu stricto Giles. In this study, foot odor of the individual with the most attractive 'smelly' feet to the An. gambiae was collected, analyzed and attractive blend components identified. Methods: The foot odor of the individual with the most attractive 'smelly' feet to the An. gambiae was trapped on Porapak Q and analyzed by …

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In Situ Nitrogen Mineralization And Nutrient Release By Soil Amended With Black Soldier Fly Frass Fertilizer, Dennis Beesigamukama, Benson Mochoge, Nicholas Korir, Changeh J. Ghemoh, Sevgan Subramanian, Chrysantus M. Tanga Dec 2021

In Situ Nitrogen Mineralization And Nutrient Release By Soil Amended With Black Soldier Fly Frass Fertilizer, Dennis Beesigamukama, Benson Mochoge, Nicholas Korir, Changeh J. Ghemoh, Sevgan Subramanian, Chrysantus M. Tanga

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Although black soldier fly frass fertilizer (BSFFF) is effective on crop performance, information on nitrogen (N) mineralization and nutrient release capacity of soils amended with BSFFF is lacking. This study utilized field incubation experiments to investigate the ammonification, nitrification, microbial populations, and quantities of nutrients released by soils amended with BSFFF and commercial organic fertilizer (SAFI) for a period equivalent to two maize cropping seasons. For the control treatment, no BSFFF or SAFI was added. Results indicated that most of the N in BSFFF amended soils was available in the ammonium form, while soils treated with SAFI had higher nitrate …

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The Dilemma Of Guinea Grass (Megathyrsus Maximus): A Valued Pasture Grass And A Highly Invasive Species, Aaron C. Rhodes, Robert M. Plowes, John A. Goolsby, John F. Gaskin, Boaz Musyoka, Paul André Calatayud, Massimo Cristofaro, Eric D. Grahmann, Dino J. Martins, Lawrence E. Gilbert Dec 2021

The Dilemma Of Guinea Grass (Megathyrsus Maximus): A Valued Pasture Grass And A Highly Invasive Species, Aaron C. Rhodes, Robert M. Plowes, John A. Goolsby, John F. Gaskin, Boaz Musyoka, Paul André Calatayud, Massimo Cristofaro, Eric D. Grahmann, Dino J. Martins, Lawrence E. Gilbert

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On a global scale, invasive grasses threaten biodiversity and ecosystem function. Nevertheless, the importation of forage grasses is a significant economic force driven by globalization. Pastureland and rangeland are of critical economic and ecological importance, but novel grass species may lead to invasion. Recognizing that economically important species can also be ecologically damaging creates a contentious debate for land managers, policymakers, and ecologists alike. Many Afrotropical perennial grass species have been intentionally introduced pantropically given their high forage production and resistance to stress. However, these traits may also confer competitive ability, increasing the possibility of unintended escape and invasion. Further, …

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Occurrence, Diversity And Distribution Of Trypanosoma Infections In Cattle Around The Akagera National Park, Rwanda, S. Richard Gashururu, Ndichu Maingi, Samuel M. Githigia, Methode N. Gasana, Peter O. Odhiambo, Dennis O. Getange, Richard Habimana, Giuliano Cecchi, Weining Zhao, James Gashumba, Joel L. Bargul, Daniel K. Masiga Dec 2021

Occurrence, Diversity And Distribution Of Trypanosoma Infections In Cattle Around The Akagera National Park, Rwanda, S. Richard Gashururu, Ndichu Maingi, Samuel M. Githigia, Methode N. Gasana, Peter O. Odhiambo, Dennis O. Getange, Richard Habimana, Giuliano Cecchi, Weining Zhao, James Gashumba, Joel L. Bargul, Daniel K. Masiga

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Background African Trypanosomiases threaten the life of both humans and animals. Trypanosomes are transmitted by tsetse and other biting flies. In Rwanda, the African Animal Trypanosomiasis (AAT) endemic area is mainly around the tsetse-infested Akagera National Park (NP). The study aimed to identify Trypanosoma species circulating in cattle, their genetic diversity and distribution around the Akagera NP. Methodology A cross-sectional study was carried out in four districts, where 1,037 cattle blood samples were collected. The presence of trypanosomes was determined by microscopy, immunolog-ical rapid test VerY Diag and PCR coupled with High-Resolution Melt (HRM) analysis. A parametric test (ANOVA) was …

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Temperature-Dependent Modelling And Spatial Prediction Reveal Suitable Geographical Areas For Deployment Of Two Metarhizium Anisopliae Isolates For Tuta Absoluta Management, Ayaovi Agbessenou, Komivi S. Akutse, Abdullahi A. Yusuf, Sospeter W. Wekesa, Fathiya M. Khamis Dec 2021

Temperature-Dependent Modelling And Spatial Prediction Reveal Suitable Geographical Areas For Deployment Of Two Metarhizium Anisopliae Isolates For Tuta Absoluta Management, Ayaovi Agbessenou, Komivi S. Akutse, Abdullahi A. Yusuf, Sospeter W. Wekesa, Fathiya M. Khamis

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Tuta absoluta is one of the most devastating pests of Solanaceae crops in Africa. We previously demonstrated the efficacy of Metarhizium anisopliae isolates ICIPE 18, ICIPE 20 and ICIPE 665 against adult T. absoluta. However, adequate strain selection and accurate spatial prediction are fundamental to optimize their efficacy and formulations before field deployment. This study therefore assessed the thermotolerance, conidial yield and virulence (between 15 and 35 °C) of these potent isolates. Over 90% of conidia germinated at 20, 25 and 30 °C while no germination occurred at 15 °C. Growth of the three isolates occurred at all temperatures, but …

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Mating Behaviour, Mate Choice And Female Resistance In The Bean Flower Thrips (Megalurothrips Sjostedti), Adeyemi O. Akinyemi, Sevgan Subramanian, David K. Mfuti, Tom W. Pope, Amanuel Tamiru, William D.J. Kirk Dec 2021

Mating Behaviour, Mate Choice And Female Resistance In The Bean Flower Thrips (Megalurothrips Sjostedti), Adeyemi O. Akinyemi, Sevgan Subramanian, David K. Mfuti, Tom W. Pope, Amanuel Tamiru, William D.J. Kirk

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Many species of thrips (Thysanoptera) in the family Thripidae form mating aggregations, but the adaptive significance of these aggregations and the extent of male and female mate choice is poorly understood. We studied the mating behaviour of the bean flower thrips Megalurothrips sjostedti (Trybom) (Thysanoptera: Thripidae), which forms male aggregations and occurs across sub-Saharan Africa. We tested whether males choose mates by female age or mating status. No-choice mating bioassays with one male and one female were used to simulate the way males usually encounter only one female at a time in aggregations in the field. Virgin females violently resisted …

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Whole Genome Comparisons Reveal Panmixia Among Fall Armyworm (Spodoptera Frugiperda) From Diverse Locations, Katrina A. Schlum, Kurt Lamour, Caroline Placidi De Bortoli, Rahul Banerjee, Robert Meagher, Eliseu Pereira, Maria Gabriela Murua, Gregory A. Sword, Ashley E. Tessnow, Diego Viteri Dillon, Angela M. Linares Ramirez, Komivi S. Akutse, Rebecca Schmidt-Jeffris, Fangneng Huang, Dominic Reisig, Scott J. Emrich, Juan Luis Jurat-Fuentes Dec 2021

Whole Genome Comparisons Reveal Panmixia Among Fall Armyworm (Spodoptera Frugiperda) From Diverse Locations, Katrina A. Schlum, Kurt Lamour, Caroline Placidi De Bortoli, Rahul Banerjee, Robert Meagher, Eliseu Pereira, Maria Gabriela Murua, Gregory A. Sword, Ashley E. Tessnow, Diego Viteri Dillon, Angela M. Linares Ramirez, Komivi S. Akutse, Rebecca Schmidt-Jeffris, Fangneng Huang, Dominic Reisig, Scott J. Emrich, Juan Luis Jurat-Fuentes

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Background: The fall armyworm (Spodoptera frugiperda (J.E. Smith)) is a highly polyphagous agricultural pest with long-distance migratory behavior threatening food security worldwide. This pest has a host range of > 80 plant species, but two host strains are recognized based on their association with corn (C-strain) or rice and smaller grasses (R-strain). The population genomics of the United States (USA) fall armyworm remains poorly characterized to date despite its agricultural threat. Results: In this study, the population structure and genetic diversity in 55 S. frugiperda samples from Argentina, Brazil, Kenya, Puerto Rico and USA were surveyed to further our understanding of …

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Satellite-Based Modelling Of Potential Tsetse (Glossina Pallidipes) Breeding And Foraging Sites Using Teneral And Non-Teneral Fly Occurrence Data, Stella Gachoki, Thomas Groen, Anton Vrieling, Michael Okal, Andrew Skidmore, Daniel Masiga Dec 2021

Satellite-Based Modelling Of Potential Tsetse (Glossina Pallidipes) Breeding And Foraging Sites Using Teneral And Non-Teneral Fly Occurrence Data, Stella Gachoki, Thomas Groen, Anton Vrieling, Michael Okal, Andrew Skidmore, Daniel Masiga

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Background: African trypanosomiasis, which is mainly transmitted by tsetse flies (Glossina spp.), is a threat to public health and a significant hindrance to animal production. Tools that can reduce tsetse densities and interrupt disease transmission exist, but their large-scale deployment is limited by high implementation costs. This is in part limited by the absence of knowledge of breeding sites and dispersal data, and tools that can predict these in the absence of ground-truthing. Methods: In Kenya, tsetse collections were carried out in 261 randomized points within Shimba Hills National Reserve (SHNR) and villages up to 5 km from the reserve …

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Global Population Genetic Structure And Demographic Trajectories Of The Black Soldier Fly, Hermetia Illucens, Cengiz Kaya, Tomas N. Generalovic, Gunilla Ståhls, Martin Hauser, Ana C. Samayoa, Carlos G. Nunes-Silva, Heather Roxburgh, Jens Wohlfahrt, Ebenezer A. Ewusie, Marc Kenis, Yupa Hanboonsong, Jesus Orozco, Nancy Carrejo, Satoshi Nakamura, Laura Gasco, Santos Rojo, Chrysantus M. Tanga, Rudolf Meier, Clint Rhode, Christine J. Picard, Chris D. Jiggins, Florian Leiber, Jeffery K. Tomberlin, Martin Hasselmann, Wolf U. Blanckenhorn, Martin Kapun, Christoph Sandrock Dec 2021

Global Population Genetic Structure And Demographic Trajectories Of The Black Soldier Fly, Hermetia Illucens, Cengiz Kaya, Tomas N. Generalovic, Gunilla Ståhls, Martin Hauser, Ana C. Samayoa, Carlos G. Nunes-Silva, Heather Roxburgh, Jens Wohlfahrt, Ebenezer A. Ewusie, Marc Kenis, Yupa Hanboonsong, Jesus Orozco, Nancy Carrejo, Satoshi Nakamura, Laura Gasco, Santos Rojo, Chrysantus M. Tanga, Rudolf Meier, Clint Rhode, Christine J. Picard, Chris D. Jiggins, Florian Leiber, Jeffery K. Tomberlin, Martin Hasselmann, Wolf U. Blanckenhorn, Martin Kapun, Christoph Sandrock

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Background: The black soldier fly (Hermetia illucens) is the most promising insect candidate for nutrient-recycling through bioconversion of organic waste into biomass, thereby improving sustainability of protein supplies for animal feed and facilitating transition to a circular economy. Contrary to conventional livestock, genetic resources of farmed insects remain poorly characterised. We present the first comprehensive population genetic characterisation of H. illucens. Based on 15 novel microsatellite markers, we genotyped and analysed 2862 individuals from 150 wild and captive populations originating from 57 countries on seven subcontinents. Results: We identified 16 well-distinguished genetic clusters indicating substantial global population structure. The data …

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Molecular Prevalence And Risk Factors Associated With Tick-Borne Pathogens In Cattle In Western Kenya, Tatenda Chiuya, Jandouwe Villinger, Daniel K. Masiga, Dickens O. Ondifu, Maurice K. Murungi, Lillian Wambua, Armanda D.S. Bastos, Eric M. Fèvre, Laura C. Falzon Dec 2021

Molecular Prevalence And Risk Factors Associated With Tick-Borne Pathogens In Cattle In Western Kenya, Tatenda Chiuya, Jandouwe Villinger, Daniel K. Masiga, Dickens O. Ondifu, Maurice K. Murungi, Lillian Wambua, Armanda D.S. Bastos, Eric M. Fèvre, Laura C. Falzon

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Background: Tick-borne pathogens (TBPs) are of global importance, especially in sub-Saharan Africa where they represent a major constraint to livestock production. Their association with human disease is also increasingly recognized, signalling their zoonotic importance. It is therefore crucial to investigate TBPs prevalence in livestock populations and the factors associated with their presence. We set out to identify TBPs present in cattle and to determine associated risk factors in western Kenya, where smallholder livestock production is important for subsistence and market-driven income. Results: Tick-borne pathogen infections in blood samples collected from cattle at livestock markets and slaughterhouses between May 2017 and …

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Distribution And Mating Type Characterization Of Chickpea Blight (Didymella Rabiei (Kov.) V. Arx) In Ethiopia, Gezahegne Getaneh, Tadele Tefera, Fikre Lemessa, Seid Ahmed, Jandouwe Villinger Dec 2021

Distribution And Mating Type Characterization Of Chickpea Blight (Didymella Rabiei (Kov.) V. Arx) In Ethiopia, Gezahegne Getaneh, Tadele Tefera, Fikre Lemessa, Seid Ahmed, Jandouwe Villinger

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We have assessed the distribution and mating type of chickpea blight (Didymella rabiei (Kov.) v. Arx) from a total of 350 and 272 fields in major chickpea (Cicer arietinum L.) growing areas of Ethiopia for two consecutive cropping seasons 2017 and 2018, respectively. The prevalence of D. rabiei was 20 % for the year 2017 and 7% for 2018. Severity range of 2−9 and incidence of 10–100 % were recorded for both seasons in only 7–20 % of the assed fields. Field prevalence of 2017 and 2018 seasons were significantly different (df = 31; p < 0.001 and df = 31; p < 0.001, respectively). Similarly, the disease prevalence of both seasons was significantly different (df = 31; p < 0.001). The disease prevalence ratio frequency was highly deviated from (1:1) for both seasons (X2 = 213.62 p= <0.001; X2 = 144.36 p = 0.01). Genomic DNA of Representative samples were extracted from single spore culture and Mating type 1 (MAT1-1) specific primer SP21, Mating type 2 (MAT1-2) specific primer Tail 5, and a flanking region-specific primer Com1 assay were multiplexed in a single PCR reaction to determine the occurrence of D. rabiei mating type in Ethiopia. Out of 156 samples, only 15 samples were positive to MAT1-1 (∼10 %) with the ratio of 9:1. MAT 1−2 type was the most dominant and possibly the asexual reproduction of D. rabiei is the major type in Ethiopia. The result is important for Ascochyta blight management in breeding strategy.

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An Update On The Distribution Of Glossina (Tsetse Flies) At The Wildlife-Human-Livestock Interface Of Akagera National Park, Rwanda, Richard S. Gashururu, Samuel M. Githigia, Methode N. Gasana, Richard Habimana, Ndichu Maingi, Giuliano Cecchi, Massimo Paone, Weining Zhao, Daniel K. Masiga, James Gashumba Dec 2021

An Update On The Distribution Of Glossina (Tsetse Flies) At The Wildlife-Human-Livestock Interface Of Akagera National Park, Rwanda, Richard S. Gashururu, Samuel M. Githigia, Methode N. Gasana, Richard Habimana, Ndichu Maingi, Giuliano Cecchi, Massimo Paone, Weining Zhao, Daniel K. Masiga, James Gashumba

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Background: Glossina (tsetse flies) biologically transmit trypanosomes that infect both humans and animals. Knowledge of their distribution patterns is a key element to better understand the transmission dynamics of trypanosomosis. Tsetse distribution in Rwanda has not been well enough documented, and little is known on their current distribution. This study determined the current spatial distribution, abundance, diversity, and seasonal variations of tsetse flies in and around the Akagera National Park. Methods: A longitudinal stratified sampling following the seasons was used. Biconical traps were deployed in 55 sites for 6 consecutive days of each study month from May 2018 to June …

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Water Immersion Tolerance By Larval Instars Of Stable Fly, Stomoxys Calcitrans, L1758 (Diptera: Muscidae) Impairs The Fitness Performance Of Their Subsequent Stages, Steve B.S. Baleba Dec 2021

Water Immersion Tolerance By Larval Instars Of Stable Fly, Stomoxys Calcitrans, L1758 (Diptera: Muscidae) Impairs The Fitness Performance Of Their Subsequent Stages, Steve B.S. Baleba

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Background: In holometabolous insects, environmental factors experienced in pre-imaginal life stages affect the life-history traits within that stage and can also influence subsequent life stages. Here, I assessed tolerance to water immersion by the larval instars of the stable fly, Stomoxys calcitrans L. (Diptera: Muscidae) and its impact on the life-history traits of their subsequent life stages. Results: After submerging the three larval instars of S. calcitrans in distilled water, I found that the first instar larvae remained active for longer as compared to the second and third instar larvae. Also, the first instar larvae took a longer period to …

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Immediate Impacts Of Covid-19 Measures On Bean Production, Distribution, And Food Security In Eastern Africa, Eileen Nchanji, Cosmas Kweyu Lutomia, David Karanja Dec 2021

Immediate Impacts Of Covid-19 Measures On Bean Production, Distribution, And Food Security In Eastern Africa, Eileen Nchanji, Cosmas Kweyu Lutomia, David Karanja

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The outbreak of coronavirus was expected to adversely affect African countries more than any other region in the world. This assertion was based on the existing conditions in sub-Saharan Africa that exposed the region to the dire consequences of the pandemic. Previously existing underlying conditions that affected the food system include a high dependence on trade for inputs supply, the adverse effects of climate change, crop pests and diseases, poverty, low input use, weak institutions and ineffective policies, and insecurity and conflicts. We collected data from farmers, aggregators, bean research coordinators, and urban and peri-urban consumers in five Eastern African …

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Molecular Detection Of Leishmania Donovani, Leishmania Major, And Trypanosoma Species In Sergentomyia Squamipleuris Sand Flies From A Visceral Leishmaniasis Focus In Merti Sub-County, Eastern Kenya, Barrack O. Owino, Jackline Milkah Mwangi, Steve Kiplagat, Hannah Njiriku Mwangi, Johnstone M. Ingonga, Alphine Chebet, Philip M. Ngumbi, Jandouwe Villinger, Daniel K. Masiga, Damaris Matoke-Muhia Dec 2021

Molecular Detection Of Leishmania Donovani, Leishmania Major, And Trypanosoma Species In Sergentomyia Squamipleuris Sand Flies From A Visceral Leishmaniasis Focus In Merti Sub-County, Eastern Kenya, Barrack O. Owino, Jackline Milkah Mwangi, Steve Kiplagat, Hannah Njiriku Mwangi, Johnstone M. Ingonga, Alphine Chebet, Philip M. Ngumbi, Jandouwe Villinger, Daniel K. Masiga, Damaris Matoke-Muhia

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Background: Visceral leishmaniasis (VL) and zoonotic cutaneous leishmaniasis (ZCL) are of public health concern in Merti sub-County, Kenya, but epidemiological data on transmission, vector abundance, distribution, and reservoir hosts remain limited. To better understand the disease and inform control measures to reduce transmission, we investigated the abundance and distribution of sand fly species responsible for Leishmania transmission in the sub-County and their blood-meal hosts. Methods: We conducted an entomological survey in five villages with reported cases of VL in Merti sub-County, Kenya, using CDC miniature light traps and castor oil sticky papers. Sand flies were dissected and identified to the …

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Gene Co-Expression Network Analysis Of Trypanosoma Brucei In Tsetse Fly Vector, Kennedy W. Mwangi, Rosaline W. Macharia, Joel L. Bargul Dec 2021

Gene Co-Expression Network Analysis Of Trypanosoma Brucei In Tsetse Fly Vector, Kennedy W. Mwangi, Rosaline W. Macharia, Joel L. Bargul

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Background: Trypanosoma brucei species are motile protozoan parasites that are cyclically transmitted by tsetse fly (genus Glossina) causing human sleeping sickness and nagana in livestock in sub-Saharan Africa. African trypanosomes display digenetic life cycle stages in the tsetse fly vector and in their mammalian host. Experimental work on insect-stage trypanosomes is challenging because of the difficulty in setting up successful in vitro cultures. Therefore, there is limited knowledge on the trypanosome biology during its development in the tsetse fly. Consequently, this limits the development of new strategies for blocking parasite transmission in the tsetse fly. Methods: In this study, RNA-Seq …

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