Life Sciences Commons^™

Assessing The Potential Of Taming Chicken Gut Commensal Bacteria Using Random Transposon Mutagenesis, Arjun Chapagain

Graduate Theses and Dissertations

The chicken gut microbiome comprises hundreds of bacterial species whose functional roles remain largely unknown, in part because the tools needed to experimentally manipulate these organisms in their native community context have not been systematically developed or validated. This dissertation addresses the gap through three connected research chapters that progressively build a framework for transposon-based genetic delivery in the chicken cecal microbiome, moving from community-wide accessibility mapping to vector optimization to library preparation refinement. In Chapter 2, we established the community-level insertion landscape in chicken cecal metagenome using Himar transposon delivery via RP4 conjugation and aligned Tn-seq reads to a …

Post-Exercise Vitals Recovery Response In Barrel Horses: A Field Study On Cooling Methods Used By Competitors, Lauran E. Hetrick

Electronic Theses and Dissertations

Barrel racing demands peak physical performance from equine athletes, yet limited research evaluates the field-based strategies used to optimize equine recovery at competition. This study assessed recovery management approaches used by competitors to elucidate their influence of physiological recovery by measuring heart rate, respiratory rate, and body temperature during a high-profile barrel race competition. Physiological data were collected at baseline, immediately post-exercise, and during a 15–30-minute cooldown period to characterize recovery under real-world conditions. Significant increases in HR, RR, and BT occurred from baseline to post-run (p < 0.001), followed by reductions during cooldown (p < 0.01), though values remained elevated (p < 0.05). Rinsing reduced HR and RR (p < 0.01), while shade and fans decreased RR and variably influenced HR. Noise, as a confounding variable, decreased HR but increased BT (p < 0.001). Ultimately, these results emphasize multimodal recovery approaches in competitive equine management in the field.

Evaluation Of Palm Oil As An Ingredient In Finishing Beef Cattle Diets On Performance, Carcass Characteristics, And Meat Quality, And The Composition Of Spayed Heifers To Intact Heifers Fed Melengestrol Acetate On Feedlot Performance And Carcass Characteristics, Tate S. Johnson

Department of Animal Science: Dissertations, Theses, and Student Research

Heifers represent a large proportion of cattle placed in feedlots, making pregnancy management an important consideration for maintaining performance and carcass value. Melengestrol acetate (MGA) is commonly included in feedlot diets to suppress estrus and prevent pregnancy in intact heifers during the finishing period. Alternatively, spaying has historically been utilized as a management practice to allow heifers to graze alongside breeding cattle without the risk of pregnancy. Despite its historical use, there is limited contemporary research comparing the growth performance and carcass characteristics of spayed heifers with intact heifers managed with MGA under modern feedlot conditions. Therefore, a commercial study …

2nd Place Research Paper: Shaping The Ape: Storytelling, Cultural Reflection, And Boundary Negotiation In Human-Ape Zoo Encounters, Abigail Bond Halling

Kevin and Tam Ross Undergraduate Research Prize

The typical Western zoo functions as a unique environment in which animals are frequently positioned as subjects of education and entertainment through the medium of storytelling. In this sense, the zoological institution can also be read as an extension of popular Western constructions surrounding nonhuman animals, particularly those who are symbolically charged characters in our collective imaginary– the great apes. Given the morphological, behavioral, and psychological similarities between humans and other great apes (chimpanzees, gorillas, and orangutans), nonhuman great ape enclosures at the Western zoo become spaces where humans are most radically confronted with navigating the boundaries of the “human-animal …

Impacts Of Supplemental Fat In Finishing Diets On Digestibility, Performance, Carcass Outcomes, Meat Quality And Methane Production Of Feedlot Cattle, Anna Marie Kobza

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Rising costs and fluctuating availability of supplemental fats has led to increased interest in alternative fat sources or feed additives that might improve utilization of supplemental fat in feedlot diets. Two separate experiments were conducted to evaluate alternative strategies to overcome high fat prices. The first experiment was a metabolism study, conducted to evaluate digestion parameters of supplemental fat sources with or without LYSOFORTE Extend. The objectives of this experiment were to evaluate LYSOFORTE Extend in beef finishing diets containing a saturated fat source (tallow) and unsaturated fat source (corn oil). Duodenally and ruminally fistulated steers were fed diets containing …

Assessment Of Goose-Beaked Whale Responsesto Mid-Frequency Active Sonar Usinga Hierarchical Hidden Markov Model, Stacy L. De Ruiter, Erin A. Falcone, Shannon N. Coates, Brenda K. Rone, David A. Sweeney, Karin A. Dolan, Susan M. Jarvis, Russel D. Andrews, Stephanie L. Watwood, Gregory S. Schorr

University Faculty Publications and Creative Works

Background

Acoustic disturbance is increasingly recognized as an ecological concern, particularly at sea. In light of marine mammal stranding events associated with use of naval mid-frequency active sonar (MFAS), which often involve goose-beaked whales (Ziphius cavirostris), research has sought to detect and characterize Ziphius behavioral responses to MFAS.

Methods

We deployed Sound and Motion Recording and Telemetry (SMRT) tags on 13 Ziphius in the Southern California Bight. The study area includes the United States Navy’s Southern California Anti-submarine Warfare Range (SOAR), and deployments overlapped temporally with training exercises including MFAS use. We used hierarchical hidden Markov models (HHMMs), which provided …

Aggression And Fish Welfare: Challenges And Opportunities, Christine M. Soper, Peter D. Dijkstra

Animal Sentience

Oldfield & Bonano’s (2024) [O&B] target article discusses the wellbeing and sociality of bony fishes. Many fish species, such as cichlids, are not only social but territorial. Aggression is normal, but territorial aggression in a captive environment can have a negative impact on welfare. Aggression-related injuries and deaths are prevalent in pet stores as well as research settings. We discuss the need to develop effective methods to minimize the negative consequences of aggression.

Geographical And Anatomical Microbiome Variation In Invasive Green Iguanas (Iguana Iguana), Amanda A. Diaz

All HCAS Student Capstones, Theses, and Dissertations

The common green iguana (Iguana iguana) was introduced to South Florida in 1965 and has since been an especially pernicious threat both environmentally and economically. This highly invasive species is also expanding in range and populations at an astonishing rate. Understanding how this reptile is able to adapt and thrive in novel environments is of imperative need in order to begin managing their disruptive populations. Notwithstanding their negative impacts and significance, the microbiome of wild populations has not yet been characterized. Here, using 16S ribosomal RNA gene sequencing, this study is the first to characterize the microbiome composition …

An Integrative Description Of Euscorpius Diagorasi Sp. N. From Rhodes, Greece (Scorpiones: Euscorpiidae), Konstantinos Kalaentzis, Francesco Frigioni, Eliza Kaitetzidou, Alessio Iannucci, Alexandros Triantafyllidis

Euscorpius

The genus Euscorpius Thorell, 1876 comprises a diverse and taxonomically challenging group of scorpions in the Mediterranean, with Greece representing one of its principal centers of diversity. In this study, we provide an integrative description of Euscorpius diagorasi sp. n., a new species from Rhodes Island, Greece. The new species is described on the basis of adult male and female morphology and mitochondrial COI sequence data. It is a small oligotrichous species characterized by a total length of approximately 21–25 mm, pale yellow to light brown coloration with darker reddish-brown pedipalps, pectinal tooth count of 8 in the male …

Effects Of Gnrh And Hcg Administration On Conception Rate And The Subsequent Estrous Cycle On Day 12 Following Artificial Insemination In Holstein Cows, Mustafa Hi̇lmi̇ Yaranoğlu, Hüseyi̇n Erdem

Turkish Journal of Veterinary & Animal Sciences

The present study investigates the effects of gonadotropin-releasing hormone (GnRH) and human chorionic gonadotropin administration on the conception rate of cows 12 days after artificial insemination (AI). Included in the study were 150 high milkyielding Holstein cows housed under the same husbandry and feeding conditions. All had calved at least once between the ages of 2.5 and 6 years, and were between 90 and 220 days in milk. In accordance with the farm’s reproductive management approach, a controlled internal drug release (CIDR) device was inserted into the vagina concurrent with a GnRH injection (day 0), and the device was removed …

Age-Related Estimates Of Genetic Parameters For Growth And Ultrasonic M. Longissimus Thoracis Et Lumborum Muscle Traits In Eşme Lambs, İbrahi̇m Cemal, Orhan Karaca, Onur Yilmaz, Nezi̇h Ata

Turkish Journal of Veterinary & Animal Sciences

The growth performance and ultrasonic M. longissimus thoracis et lumborum (LTL) muscle traits of 2397 Eşme lambs raised in the Eşme district of Uşak province, an important lamb production area in Western Anatolia, were evaluated at 3 months (n = 1690) and 5 months (n = 707) of age. The average age of the lambs at the time of the first measurement (at weaning) was 100.15 days, and 152.37 days at the second. At 3 months of age, the average live weight (LW) was 29.34 kg and the average daily gain (ADG) was 248.35 g/day. By month 5, the LW …

Housing System And Gender Effect On Carcass And Meat Quality Characteristics In Turkish Pekin Ducks, Hasan Önder, Uğur Şen, Mi̇ne Yilmaz, Si̇bel Bozkurt, Eli̇f Ci̇lavdaroğlu, Di̇lek Gökçek, Ahmet Uçar, Kadi̇r Erensoy, Burcu Kurnaz

Turkish Journal of Veterinary & Animal Sciences

Improving meat quality is one of the most important aims for scientists and breeders. This study aimed to evaluate the effects of housing system (indoor vs. free-range) and gender on carcass and meat quality traits in Pekin ducks. The animal material consisted of 20 male and 20 female birds in each housing system. Our results show cold carcass weights of 3174.10 ± 80.65 g and 2672.60 ± 59.63 g for male and female birds in the indoor housing system and 2821.10 ± 50.53 g and 2306.90 ± 43.32 g for male and female birds in the free-range housing system. The …

Effects Of Preslaughter Fasting On Ph And Bacterial Contamination In Akkaraman And Anatolian Merino Sheep, Sai̇m Boztepe, Özcan Şahi̇n, Talha Demi̇rci̇, Gi̇zem Coşkun, İbrahi̇m Ayteki̇n, İsmai̇l Keski̇n

Turkish Journal of Veterinary & Animal Sciences

The present study investigates the effects of the preslaughter fasting on pH and bacterial contamination in Akkaraman and Anatolian Merino sheep. Included in the study were 10 Akkaraman and 10 Anatolian Merino sheep (n = 20) that were fasted for 24 h prior to slaughter, and a further 10 Akkaraman and 10 Anatolian Merino sheep (n = 20) that continued to be fed until slaughter as a control group. The total mesophilic aerobic bacteria (TMAB) measured at 0 and 24 h in the fasting Akkaraman and Anatolian Merino sheep were 4.87/4.79 and 4.75/5.57 log CFU/g, respectively, and the corresponding Enterobacteriaceae …

The Effects Of Sodium Perborate Tetrahydrate On Digestibility And Nutrient Transporters In Rats, Büşra Özmen, Emre Şahi̇n, Muhammed Tokmak, Veysi̇ Kayri̇, Ramazan Özmen, Kazi̇m Şahi̇n

Turkish Journal of Veterinary & Animal Sciences

Boron (B) is an essential micronutrient involved in hormone activity, energy metabolism, antioxidant defense, bone development, wound healing, and immune function. The present study explores the effects of sodium perborate tetrahydrate (SPT) on nutrient digestibility and the modulation of specific nutrient transporters in the jejunum and ileum of rats. A total of 40 rats were divided into four equal groups: 1) control, 2) SPT 0.5 (0.5 mg B/kg body weight [BW]), 3) SPT 1 (1 mg B/kg BW), and 4) SPT 2 (2 mg B/kg BW), and were fed the respective diets for a period of 4 weeks. The digestibility …

Morphology And Histology Of The Developing Ovary In Malaysian Village Chicken (Gallus Gallus Domesticus) Chicks, Nur Athirah Lope Abdul Rashid, Mazlina Mazlan, Intan Shameha Abdul Razak, Rozaini Mohd Zohdi, Awang Hazmi Awang Junaidi

Turkish Journal of Veterinary & Animal Sciences

The Malaysian village chicken (MVC; Gallus gallus domesticus) is an indigenous breed derived from the red junglefowl (Gallus gallus), exhibiting slower growth and reproductive rates. The MVC plays an important ecological role in local farming systems and represents a valuable genetic resource. However, detailed information on ovarian development in this breed remains limited. This study provides the first comprehensive morphological and histological characterization of the developing ovary in MVC chicks. A total of 20 chicks aged 1, 2, 4, and 8 weeks (n = 5 per age group) were examined. These MVC chicks possessed a single functional left ovary positioned …

Effect Of Different Pellet Binders On Palm Kernel On Growth Performance And Nutrient Digestibility In Broiler Chicken, Nuun Marfuah, Burhanudin Sundu, Rusdi Rusdi, Sugiarto Sugiarto

Jurnal Ilmu Ternak Universitas Padjadjaran

Feed plays a paramount role in supporting growth, metabolism, and production performance. Their high price encourages the utilization of inexpensive and alternative feed ingredients. PKM, one such feed ingredient, is produced by palm oil industries. However, there is still a very limited application because of its high crude fiber content, which needs processing technologies like pelletization with binder addition. Based on this background, this experiment has been conducted to study the effect of various types of natural and commercial binders in 15% PKM-based pellets on performance and nutrient digestibility of broilers. The study was done for five weeks using 150 …

Evaluating Sand Characteristics And Their Influence On Sea Turtle Nest Incubation Conditions, Thomas Naylor

Honors Theses

The embryonic development of sea turtles is strongly influenced by the environmental factors experienced during incubation within the nest. These environmental factors can influence hatchling sex ratios, body condition, and their success emerging from nests. Adult female sea turtles lay nests across a range of sites throughout the nesting season, resulting in each nest encountering unique abiotic conditions. This study aims to identify how variation in sand characteristics, such as grain size proportions, permeability, and color, influences nest temperature and subsequent hatchling outcomes of Loggerhead Sea Turtles (Caretta caretta) and Green Sea Turtles (Chelonia mydas) from …

Feeding Strategies And Milking Behavior In Robotic Milking Systems, Katherine M. Kennedy, Elizabeth French, Porter D. Ricks, Emma K. Lundgreen

Animal, Dairy, and Veterinary Science Faculty Publications

Adoption of automatic milking systems (AMS) in the US are expected to increase rapidly in the coming years driven primarily by a desire to improve labor efficiency and flexibility and decrease reliance on hired labor. As such, feeding and milking strategies to best optimize milk production and economic gains tailored to US dairy production systems are needed. Initially introduced as a method to encourage the cow to visit the AMS more frequently, offered feeds through the AMS provide an opportunity to individualize nutrient needs. Often, a pelleted concentrate is offered through the AMS due to its uniformity and customizable nature. …

Continuous Light And Pinealectomy: Two Models Of Melatonin Deficiency Revealing Distinct Interactions With Vitamin D Deficiency On Blood Parameters, Rebaz Sedeeq Shekh, Ismail Mustafa Maulood

Polytechnic Journal

Modern lifestyles reduce sunlight exposure and increase artificial light at night, potentially promoting vitamin D deficiency (VDD) and melatonin (MEL) suppression. The two hormones regulate immunity, erythropoiesis, and platelet (PLT) indices, but their combined hematological effects are not well characterized. We investigated the independent and combined effects of VDD and MEL deficiency on leukocyte profiles, erythrocyte indices, and PLT parameters in albino rats using two 12-week models (n = 8/group): continuous light (CL)-induced MEL suppression (Control, CL, VDD, and CL + VDD) and pinealectomy (Px)-induced MEL deficiency (Sham. Px, VDD, Px + VDD). Complete blood counts (CBCs) and inflammation-related ratios …

Exploring Decision-Making In Caenorhabditis Elegans: Navigating Dichotomous Envrionmental Cues, Hunarmeet K. Gill

Caroliniana Undergraduate Research Journal

All living organisms thrive off the survival choices they make and the factors they prioritize, but the mechanisms guiding these choices remain unclear. This study investigates decision-making in Caenorhabditis elegans, aiming to understand how they navigate dichotomous environments that contain both attractive and aversive cues. Despite possessing a simple nervous system, C. elegans express behaviors indicating a level of advanced sensory-processing abilities, making them ideal models for studying decision-making processes. The experiment consisted of five separate petri-dish tests, each with three sections: side A and side B (containing natural or UV light and/or E.coli or ethanol), and a neutral …

Evaluating Elisa-Based Domoic Acid Detection Methods In Fetal And Maternal Samples Of California Sea Lions (Zalophus Californianus), Deanie Woodruff, Emily Lickiss, Samuel Gardiner, Mia Gastile, Elizabeth Sandoval, Florybeth La Valle

Seaver College Research And Scholarly Achievement Symposium

Harmful algal blooms produced by the diatom Pseudo-nitzschia regularly expose marine mammals along the California coast to domoic acid (DA), a neurotoxin that bioaccumulates in marine food webs. In California sea lions (Zalophus californianus), domoic acid exposure is associated with seizures, hippocampal injury, and maternal-fetal transfer of the toxin that can result in abortion. Bloom events have led to large numbers of stranded sea lions along the California coast. Although some clinical and neurological effects of domoic acid toxicosis have been documented, especially with regard to the hippocampus, there is limited understanding of how domoic acid concentrations fluctuate over the …

Hot Cities, Cool Lizards: Do College Campuses Create Urban Oases For Wildlife?, Ethan Heck, Javier Monzon Phd

Seaver College Research And Scholarly Achievement Symposium

Urban wildlife may experience stress due to the Urban Heat Island Effect. On the other hand, urban wildlife may experience relief, especially in arid ecosystems, due to the Urban Oasis Effect. The objective of this study was to test whether college campuses in southern California act as heat islands or oases for wildlife. We analyzed how temperature and water availability of microhabitats relate to the physiology of a widespread and common cold-blooded animal, the Western Fence Lizard (Sceloporus occidentalis). We logged environmental conditions in lizard hotspots at 11 university campuses across southern California and measured the hydration of lizards at …

Forest Characteristics And Avian Community Differences Among Cerulean Warblers (Setophaga Cerulea) Use Types, Dustin Kohler

ATU Scholars Symposium

Avian species are often used to measure the health of an ecosystem, in part because the specific forest structure can significantly impact the avian community. A heterogeneous landscape can support a more diverse avian community and benefit specialist species like the Cerulean Warbler (Setophaga cerulea), a rapidly declining, neotropical migrant that can be found in large deciduous forests. The Ozark Mountains of northwestern Arkansas represent a small core area for the species, but at all sites in the upland Ozarks where Cerulean Warbler research has been conducted, there are fewer individuals than there were twenty years ago; some …

Population Loss And Site Abandonment Of Cerulean Warblers (Setophaga Cerulea) In The Ozarks, Brayden D. Johnson, Dustin Kohler

ATU Scholars Symposium

Cerulean Warblers (Setophaga cerulea) have declined across their breeding range, including the Ozark Mountains of Arkansas, where historically occupied sites have experienced reduced populations or abandonment. Understanding population trends and habitat structure is important for informing forest management and conservation. This study compares historic and current Cerulean Warbler counts at selected upland sites within the Ozark-St. Francis National Forest and evaluates whether differences in canopy composition between abandoned historic sites and foraging sites currently used by Cerulean Warblers can explain site abandonment. Population trajectories varied among sites. Cherry Bend remained consistently occupied from 1998-2004 with 16-24 individuals and supported 17 …

Comprehensive Temporal, Spatial, And Environmental Analysis Of Arkansas Tournament Information Program (Atip) Data With A Lake Dardanelle Case Study., Jeffrey G. Phillips

ATU Scholars Symposium

The Arkansas Game and Fish Commission (AGFC) established the Arkansas Tournament Information Program (ATIP) in 1990 to provide a long-term, standardized dataset to describe Black Bass (Micropterus spp.) tournament outcomes, angler performance, and fish size structures across Arkansas, yet most of its analytical potential has not been fully explored beyond yearly summary reports. This project will use ATIP data from 1990 to 2027 and a biological case study from Lake Dardanelle to evaluate long-term trends, compare lake-specific performance, identify environmental drivers of tournament success, access how well tournament data represents true Black Bass populations, and create an “average” year benchmark …

Grassland Bird Community Responses To Fire Management, Mason H. Dillard

ATU Scholars Symposium

Vegetation structure plays a major role in determining avian community makeup. Controlled burn frequency changes vegetation structure and consequently the makeup of avian communities. To examine this relationship, I surveyed breeding birds in grasslands and savannahs of the Ozark Mountains, Arkansas River Valley, and Northwest Arkansas. I created models to outline the relationship between these three factors (avian community, fire management, and vegetation structure.) I also examined surrounding land cover and patch size as potential variables that may impact avian community structures.

I will outline the role fire management plays in determining the makeup of breeding bird communities in grasslands, …

Fish Community Assessment Of Goose Pond Natural Area, Macy Sparks

ATU Scholars Symposium

Natural areas play an integral role in the preservation and conservation of unique ecosystems (i.e., natural wetlands). However, many natural areas have never had a biological inventory of species utilizing these areas. Goose Pond Natural Area (Conway County and Pope County, Arkansas) is one of those natural areas lacking species occurrence data. We addressed this data gap by sampling fish species across multiple habitat types (shallow woody wetland, open grassy wetland, and stream). We employed multiple gear types (mesh umbrella minnow traps, seines, gill nets, mini-fyke nets, and backpack electrofishing) in hopes of increasing species detection. Sites within the natural …

Bluebird Bait: Quantifying The Attraction Of Ectoparasites To Nestling Eastern Bluebirds (Sialia Sialis), Ella Grace Bollinger

ATU Scholars Symposium

Ectoparasites, such as mosquitoes (Culicidae), blackflies (Simuliidae), and biting midges (Ceratopogonidae), have been shown to reduce population viability by lowering post-fledging survival and decreasing the lifetime reproductive success of adult birds. This issue is particularly concerning for songbirds such as the Eastern Bluebird (Sialia sialis) because their nestlings are relatively naked and have limited mobility, making them highly vulnerable to attacks by biting flies. However, the specific mechanisms attracting ectoparasites to nests remain understudied. We hypothesized that biting fly and mosquito abundance would be significantly higher at active Eastern Bluebird nests than that of empty boxes and would increase with …

Assessing The Effect Of A Human-Made Reservoir On The Mussel Community In Caney Creek, Trakker Estes

ATU Scholars Symposium

Freshwater mussels are one of the most threatened taxonomic groups globally, with less than 32% of North American taxa considered secure. In Arkansas, over half of the state’s 85 species are endangered, threatened, or of special concern. Small impoundments may contribute to these declines through reduced hydrological connectivity and the resulting decline of fish community movement. This study examines the effects of Tri-County Lake, a small impoundment of Caney Creek in southern Arkansas, on mussel abundance and diversity. In total, eight sites were surveyed upstream, within, and downstream of the impoundment across various habitat types. A timed-search approach utilizing scuba …

Colostrum Fatty Acid Composition And Its Association With Reproductive Traits In Sows Under Tropical Conditions, Dwi Sunu Datrianto, Kunaporn Homyog, Surasak Jittakhot, Padet Tummaruk

The Thai Journal of Veterinary Medicine

Colostrum provides essential nutrients and immune factors that newborn piglets can absorb only within the first 24 h after birth. Fatty acids are essential for neonatal physiology, supporting thermoregulation, neurodevelopment, and immune function, and serving as a key energy source to reduce hypothermia and mortality in piglets. However, information on the fatty acid composition of sow colostrum under tropical conditions and its relationship with reproductive performance remains limited, warranting further investigation to improve piglet survival. This study evaluated the fatty acid profile of sow colostrum under tropical production conditions and examined its associations with reproductive traits. Colostrum samples were collected …