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Articles 571 - 600 of 1308
Full-Text Articles in Mechanical Engineering
Controlled Drug Delivery And Cellular Activities From Electrospun Fibers, Diala Bani Mustafa
Controlled Drug Delivery And Cellular Activities From Electrospun Fibers, Diala Bani Mustafa
Mechanical Engineering Theses
Electrospun drug-eluting fibers have demonstrated potential applications in topical drug delivery, where modulations of drug releases were achieved through polymer fiber compositions. In this study, blend fibers of polycaprolactone (PCL) and polyethylene oxide (PEO) at various compositions were electrospun from 10 wt% of polymer solutions to encapsulate a model drug of ibuprofen (IBP). Results showed that average polymer solution viscosities determined the electrospinning parameters and the resulting average fiber diameters. In vitro release of IBP suggested a transition from a gradual release to a fast release when increasing PEO contents in the PCL/PEO blend fibers up to 120 minutes. In …
Impact Force Helmet Device For Concussion Monitoring, Patrice Mulligan, Karina Mealey, Zack Borthwick, Benjamin Fick
Impact Force Helmet Device For Concussion Monitoring, Patrice Mulligan, Karina Mealey, Zack Borthwick, Benjamin Fick
General Engineering
To track head trauma in football, our device is placed inside a football helmet to measure both linear and gyroscopic (rotational) accelerations. These measurements are taken using an ADXL375 linear accelerometer and an LSM6DSOX accelerometer and gyroscope. The data from these sensors is processed and transmitted wirelessly by a SparkFun Thing Plus microcontroller to another SparkFun Thing Plus microcontroller connected to a laptop on the sidelines. Our program on the laptop stores the acceleration impact data into a .csv file for each player. If a likely concussive impact is detected, the program displays a popup warning. Testing results were promising …
Smart Manufacturing For Cal Poly's Ime Curriculum, Grace L. Guarraia, Eric F. Ledesma
Smart Manufacturing For Cal Poly's Ime Curriculum, Grace L. Guarraia, Eric F. Ledesma
Industrial and Manufacturing Engineering
California Polytechnic State University, San Luis Obispo (Cal Poly) takes pride of supporting one of the nation’s strongest undergraduate Industrial and Manufacturing Engineering programs. However, as industry evolves to accommodate advancing computing power, improved manufacturing algorithms, and improved robotics, it is imperative that Cal Poly’s curriculum evolves in parallel. Smart manufacturing is the future of the manufacturing industry, and Cal Poly is searching for the ideal method to teach and integrate Smart Manufacturing into its classes. With its upcoming switch to the semester system, coupled with its “Learn by Doing” motto, the department is at an ideal period for the …
3d-Printed Remote Controlled Aircraft, Sebastian Bessoudo, Jose Ramirez, Manny Ruiz, Andrew Edwards
3d-Printed Remote Controlled Aircraft, Sebastian Bessoudo, Jose Ramirez, Manny Ruiz, Andrew Edwards
Mechanical Engineering
The Senior Project for the 3D Printed Aircraft presents a compelling challenge to the team: design and fabricate an aircraft for the Cal State LA 3D-Printed Fixed-Wing Aircraft Competition, comprising three distinct events: Flight, Design, and Simulation. The objective of the flight competition is to achieve the longest flight duration, while the design event emphasizes innovation and originality. In the Simulation contest, our aircraft's CAD model undergoes Computational Fluid Dynamics analysis to assess its aerodynamic performance. These competitions are slated for May 2024.
While building the aircraft has its limitations due to the source of our construction being only 3D …
Snap It & Send It, Samantha Gonzales, Brienne Berger, Kelsea Clays, Jessica Schmok
Snap It & Send It, Samantha Gonzales, Brienne Berger, Kelsea Clays, Jessica Schmok
Mechanical Engineering
This report details the final design, manufacturing and assembly, testing procedures and results, as well as future design recommendations of a device aimed at enhancing the mountain biking experience for individuals with limited thumb strength. This project particularly focused on the experience of our sponsor, Cal Poly Mechanical Engineering Professor Andrew Kean. Our team's objective was to create a secure, ergonomic handlebar grip system that does not require thumb strength and is adaptable to various mountain bike models. The final design utilizes a rigid hook that snaps onto mountain bike handlebars with a long, flat piece that slides into a …
Automated Expanding Fireplace, Fermin Moreno, Eduardo Hernandez, Wilbert Quiterio, Ben Hoefer
Automated Expanding Fireplace, Fermin Moreno, Eduardo Hernandez, Wilbert Quiterio, Ben Hoefer
Mechanical Engineering
The Final Design Review (FDR) encompasses information regarding the final design verification prototype (FVP), manufacturing, testing, and future recommendations for the Expanding Fireplace Senior Project sponsored by the SLO Civic Ballet. The project aims to create the frame of an expanding fireplace set piece for The Nutcracker production at the Cal Poly Performing Arts Center in December 2024. The current set piece being used has unfavorable features that will be addressed with the new design. The current set piece being used has unfavorable features that have been addressed with the new design we have developed. Our sponsors have proposed several …
Miniature Train Engine, Jason Patrick Lacson Del Castillo, Renato Andrew Dilauro, Sophia Theresa Tiu, Robert John Pallares Iii
Miniature Train Engine, Jason Patrick Lacson Del Castillo, Renato Andrew Dilauro, Sophia Theresa Tiu, Robert John Pallares Iii
Mechanical Engineering
This report details the redesign of the Mini Train Prop for the SLO Civic Ballet’s The Nutcracker, aiming to create a lighter, more user-friendly, and easily maneuverable train. The Final Design Review (FDR) includes the design process, manufacturing plan, and justifications for decisions made. Key improvements are reducing the train's weight, automating the fake wheels, and enhancing maneuverability with a neutral pin and lever system. In this report we detail the significant changes since the Critical Design Review (CDR). The implementation covers procurement, manufacturing, and assembly, while design verification ensures compliance with cost, weight, turn radius, and structural integrity specifications. …
Bird Trail Stairway Access Replacement, Mackenzie Wright, William Spellman, Nicholas Buccino, Jack Long
Bird Trail Stairway Access Replacement, Mackenzie Wright, William Spellman, Nicholas Buccino, Jack Long
Mechanical Engineering
Dana Adobe & Cultural Center, a non-profit organization in Nipomo, California, seeks to restore access to the Bird Trail located on their site. This project aims to design and manufacture a replacement stairway for safe creek bed crossing on the trail. Within this document, we have detailed our system design, function, and justification for our design. The procurement, manufacturing, and assembly plan have been outlined along with tests we completed.
One of the most important functions of our design is for the structure to be retractable so that it can be removed from the creek bed when needed. To address …
Facility Thermal Balance And Transport, Giovanni V. Minnite, Silas O R Goldfarb, Cole J. Houston, Dominique Aguilera, Cole M. Silva
Facility Thermal Balance And Transport, Giovanni V. Minnite, Silas O R Goldfarb, Cole J. Houston, Dominique Aguilera, Cole M. Silva
Mechanical Engineering
This Final Design Review (FDR) report outlines the senior design project completed by a team of five mechanical engineering students at California Polytechnic State University, San Luis Obispo for Senior Industrial Engineer, Mr. Tom Bass, of Entegris, Inc. The team developed an innovative system to address the issue of ice accumulation on cryogenic vaporizers at the Entegris facility. Ice buildup, caused by the extremely low temperatures of the cryogenic fluid, reduces the efficiency of vaporizers and places additional stress on the system. To combat this, the team designed and constructed a prototype that utilizes a thermal transport loop to transfer …
F21 - Variable Damper, Harrison Pomerantz, Anthony Mendoza, Owen Wilks
F21 - Variable Damper, Harrison Pomerantz, Anthony Mendoza, Owen Wilks
Mechanical Engineering
This document outlines the final design phase for the Solar Turbines Variable Damper project, aiming to enhance the safety and serviceability of gas turbines enclosure ventilation system. The primary focus of this project is to address the challenge of maintaining optimal internal pressure within the gas generator enclosures while enabling remote control of the damper position.
In this report, the full system design is described in detail. Then, the part procurement, manufacturing, and assembly of the variable damper and test stand is provided to outline the steps and processes taken to complete the final prototype. After discussing the final prototype, …
Enhancing Miter Saw Safety: Challenges And Prospects Of Brake Incorporation With An Automatic Blade Retraction System, Derek Cole
University Honors Theses
Miter saws pose significant safety risks with thousands of injuries reported annually. Despite this risk, they are still used every day as they are a necessity for thousands of workers in the United States and globally. This leaves a demand to make these machines safer to mitigate any potential harm. This research addresses the critical need for an automatic safety system for miter saws, aiming to enhance user safety by retracting and stopping the blade upon skin contact. This analysis identified the primary challenge: The large force generated when braking at high speed on a miter saw blade, translates the …
Design And Assembly Of A Variable Buoyancy System For An Autonomous Underwater Vehicle, Evan Brown
Design And Assembly Of A Variable Buoyancy System For An Autonomous Underwater Vehicle, Evan Brown
Master's Theses
Autonomous underwater vehicles (AUVs) play a vital role in the surveillance and mapping of our oceans, often requiring extensive travel in extreme environments. These data-gathering missions benefit from extended durations, making the minimization of power consumption a key consideration in AUV design. One effective strategy to reduce energy use in AUVs is to implement a variable buoyancy system (VBS) for depth control. The purpose of this work is to design, develop, test, and model a variable buoyancy system to enhance the efficiency and capabilities of a high-speed, long-range AUV.
After evaluating various VBS designs, a piston-cylinder buoyancy system was selected …
Non-Linear Modeling Of Hysteresis In Piezoelectric Actuated Cantilever Beam Using The Bouc-Wen Model, Andrew Donald Maas
Non-Linear Modeling Of Hysteresis In Piezoelectric Actuated Cantilever Beam Using The Bouc-Wen Model, Andrew Donald Maas
Master's Theses
Piezoelectric actuators frequently exhibit a time-dependent behavioral phenomenon known as hysteresis, resulting in a lag in the deformation of the actuator compared to linear models. The presence of hysteresis complicates control systems involving piezoelectric actuators. However, traditional modeling methods for piezoelectric actuated smart structures often treat the piezoelectric patches as linear actuators without considering hysteresis, leading to suboptimal controller performance.
This thesis aims to establish a comprehensive model by integrating the Euler-Bernoulli beam bending model with the hysteresis dynamics induced by two opposing piezoelectric patches attached to a beam. A model expansion method is employed to transform the partial differential …
Feasibility And Characterization Of Leak-Tight Single-Track Thin Walls Produced By Laser Powder Bed Fusion In 316l Stainless Steel, Peyton J. Archibald
Feasibility And Characterization Of Leak-Tight Single-Track Thin Walls Produced By Laser Powder Bed Fusion In 316l Stainless Steel, Peyton J. Archibald
Master's Theses
This thesis explores the optimization of process parameters for producing single-track thin walls using Laser Powder Bed Fusion (LPBF) additive manufacturing. Using two different coupon designs, the study assesses the feasibility of creating the thinnest possible leak-tight structures within LPBF and evaluating their mechanical properties, including burst pressure and modulus of elasticity under pressure loads. A series of experimental iterations were conducted, varying laser power and laser speed to identify optimal conditions. The findings indicate that a narrow range of process parameters can produce consistently leak-tight thin walls. The results contribute to understanding how to achieve high quality, reliable thin …
The Pressure Distribution Of Rotating Cylinders Using An Onboard Wireless Data Acquisition System, Nathan Eller
The Pressure Distribution Of Rotating Cylinders Using An Onboard Wireless Data Acquisition System, Nathan Eller
Master's Theses
This thesis presents a novel, cost-effective method for mapping the pressure distribution on a rotating cylinder in cross flow, a phenomenon central to the Magnus effect. Utilizing commercial-off-the-shelf (COTS) micro-electromechanical system (MEMS) pressure sensors, a high-resolution data acquisition system was developed and integrated into a rotating cylinder model. Compared to traditional approaches, such as slip rings or one-off designs, this method proved significantly cheaper and faster while achieving comparable or superior resolution. The experimental setup, including a modified continuous rotation technique and adaptable model design, facilitated rapid testing across a broad range of Reynolds numbers and reduced frequencies, exceeding the …
Product Design For Repairability: Identifying Failure Modes With Topic Modeling And Designing For Electronic Waste Reuse, Claire J. Franz
Product Design For Repairability: Identifying Failure Modes With Topic Modeling And Designing For Electronic Waste Reuse, Claire J. Franz
Master's Theses
Design for repairability is imperative to making products that last long enough to justify the resources they consume and the pollution they generate. While design for repairability has been gaining steady momentum, especially with recent advances in Right to Repair legislation, there is still work to be done. There are gaps in both the tools available for repair-conscious designers and the products coming onto store shelves. This thesis work aims to help set sails in the right direction on both fronts.
This research explores the use of topic modeling (a natural language processing technique) to extract repairability design insights from …
Development Of A Deep Learning Algorithm Using Electromyography (Emg) And Acceleration To Monitor Upper Extremity Behavior With Application To Individuals Post-Stroke, Nathan Dodd
Master's Theses
Stroke is a chronic illness which often impairs survivors for extended periods of time,
leaving the individual limited in motor function. The ability to perform daily activities
(ADL) is closely linked to motor recovery following a stroke. The objective of
this work is to employ surface electromyography (sEMG) gathered through a novel,
wearable armband sensor to monitor and quantify ADL performance. The first contribution
of this work seeks to develop a relationship between sEMG and and grip
aperture, a metric tied to the success of post-stroke individuals’ functional independence.
The second contribution of this work aims to develop a deep …
Interaction Of Particles With Plasma And Shock Produced By Pulsed Spark Discharge, Shomik Mukhopadhyay
Interaction Of Particles With Plasma And Shock Produced By Pulsed Spark Discharge, Shomik Mukhopadhyay
Dissertations
Interactions of powders with high-temperature plasma and shockwaves occur in diverse scenarios, such as nuclear blasts, accidental industrial dust explosions, solid propellant combustion in explosive charges and when removing contaminants from surfaces. Electrostatic Discharge (ESD), known for generating shock and plasma, is a promising lab-scale technique for simulating these interactions. Studies with ESD involved placing powders near a spark-producing gap between electrodes and observing mechanical and chemical processes like particle motion and ignition. A limited range of spark conditions and material properties have been tested, which facilitated the development and validation of preliminary computational models describing this system. Significant gaps …
Reproducible Fluid Structure Interaction For Complex Flows In Engineered Systems, Allen Prasad Varghese
Reproducible Fluid Structure Interaction For Complex Flows In Engineered Systems, Allen Prasad Varghese
Dissertations
This dissertation focuses on the application of numerical models to study Fluid Structure Interaction (FSI) in the industrial context. The emphasis is on a series of case studies belonging to practical applications in design and manufacturing and that are solved by a synergistic use of theoretical and computational models. The first study looks into the hydrodynamic actions experienced by vibrating slender structures immersed in viscous fluids near the free surface. This study case is relevant to applications such as, for example, marine propulsion, micro-electro-mechanical systems, and atomic force microscopy. The hydrodynamic problem is governed by three parameters, namely the frequency, …
Impact Of Polymer Structure In Polyurethane Topcoats On Anti-Icing Properties, Donghyeon Lee, Junho Park, Min Ji Woo, Juhyeong Lee, Joung-Man Park, Hyung Mi Lim, Tae Kyung Lee, Seong Baek Yang, Sang Yong Nam, Dong-Jun Kwon
Impact Of Polymer Structure In Polyurethane Topcoats On Anti-Icing Properties, Donghyeon Lee, Junho Park, Min Ji Woo, Juhyeong Lee, Joung-Man Park, Hyung Mi Lim, Tae Kyung Lee, Seong Baek Yang, Sang Yong Nam, Dong-Jun Kwon
Mechanical and Aerospace Engineering Faculty Publications
Icing on the topcoat layer of structures or mobility systems can be a factor leading to functional failures or accidents. Material engineering approach to prevent icing involves creating hydrophobic surfaces. In this study, it was confirmed that the method of controlling the structure of polymers using solvents to adjust surface hydrophobicity and ice prevention effects is effective. Polyurethane (PU) topcoats are primarily used on the exterior of mobility devices; therefore, structure of PU was manipulated using xylene. Through the adjustment of the ratio between PU and xylene, changes in the curing enthalpy and crystal structure were observed, which led to …
Passive Mechanical Properties Of Cardiac And Arterial Tissue: A Paired Comparison Study In A Calf Model, Ola Alsaadi, Jieyang Zhang, Masod Sadipour, Ali N. Azadani
Passive Mechanical Properties Of Cardiac And Arterial Tissue: A Paired Comparison Study In A Calf Model, Ola Alsaadi, Jieyang Zhang, Masod Sadipour, Ali N. Azadani
DU Undergraduate Research Journal Archive
Purpose: The mechanical behavior of heart tissue plays a crucial role in elucidating cardiac physiology and pathophysiology. Accurate measurement of the mechanical properties of cardiac and arterial tissues is indispensable for advancing computer simulations and developing medical devices. This study aimed to assess the passive mechanical properties of cardiac and arterial tissue in a calf model using a paired comparison method.
Methods: A planar biaxial stretching device was used to determine the mechanical characteristics of 84 square specimens obtained from 14 different anatomical regions. Cauchy stress-Green strain curves were constructed for each anatomical area, and a Fung-type constitutive …
Impact Of Student Beliefs And Self-Efficacy On Performance In Higher Education Stem Courses, Lauren Nicole Fogg
Impact Of Student Beliefs And Self-Efficacy On Performance In Higher Education Stem Courses, Lauren Nicole Fogg
Doctoral Dissertations
In engineering education, students often face feelings of inadequacy, leading to academic struggles and potential dropout. This dissertation investigates the impact of interactive course materials on students' confidence and self-efficacy in problem-solving, focusing on an Engineering Materials class at Louisiana Tech University. Over four quarters, involving seven sections and 218 students, a 13-question Likert scale survey was administered repeatedly, alongside demographic data and textbook usage surveys. The study aims to compare students’ attitudes and beliefs when not using a textbook versus when using an interactive web-native book. Hypotheses suggest that the interactive book will enhance problem-solving beliefs, confidence, and grades. …
A Computational Investigation Of Wood Selection For Acoustic Guitar, Jonah Osterhus
A Computational Investigation Of Wood Selection For Acoustic Guitar, Jonah Osterhus
Senior Honors Theses
The acoustic guitar is a stringed instrument, often made of wood, that transduces vibrational energy of steel strings into coupled vibrations of the wood and acoustic pressure waves in the air. Variations in wood selection and instrument geometry have been shown to affect the timbre of the acoustic guitar. Computational methods were utilized to investigate the impact of material properties on acoustic performance. Sitka spruce was deemed the most suitable wood for guitar soundboards due to its acoustic characteristics, strength, and uniform aesthetic. Mahogany was deemed to be the best wood for the back and sides of the guitar body …
Me-Em Enewsbrief, Mar 2024, Department Of Mechanical Engineering-Engineering Mechanics, Michigan Technological University
Me-Em Enewsbrief, Mar 2024, Department Of Mechanical Engineering-Engineering Mechanics, Michigan Technological University
Department of Mechanical and Aerospace Engineering eNewsBrief
No abstract provided.
Distributed Conflict Detection And Optimal 4d Trajectory Resolution Leveraging Polynomial Based Methods, Michael Klinefelter, Austin Stone, Joshua Miller, Cameron K. Peterson, John Salmon
Distributed Conflict Detection And Optimal 4d Trajectory Resolution Leveraging Polynomial Based Methods, Michael Klinefelter, Austin Stone, Joshua Miller, Cameron K. Peterson, John Salmon
Student Works
This paper presents a methodology for distributed conflict detection and resolution of aircraft following time-dependent flight paths. We use parametric fifth-order polynomial splines to define the full, time-based paths of vehicles. This representation can be exploited to rapidly detect conflicts and calculate optimal resolution solutions that minimize deviations from the original path. Conflicts are identified using a Sturm sequencing procedure and resolutions are found using gradient-based optimization techniques. Simulations show the locally optimal resolution of complex multi-vehicle conflicts and large-scale scenarios. Also, a method of fitting the flight path model to data sets is presented and flight path trajectories are …
Prediction Of Defects In Deep Drawn Rectangular Parts Using Finite Element Analysis (Fea) And Response Surface Methodology (Rsm), Mustafa M. Semman Eng., Mostafa Shazly, Mohamed H. Gadallah, Tamer Adel Mohamed, Abdallah S. Wifi
Prediction Of Defects In Deep Drawn Rectangular Parts Using Finite Element Analysis (Fea) And Response Surface Methodology (Rsm), Mustafa M. Semman Eng., Mostafa Shazly, Mohamed H. Gadallah, Tamer Adel Mohamed, Abdallah S. Wifi
Mechanical Engineering
Abstract. Deep drawing defects such as thinning and earing present challenges to sheet metal forming industry while designers tend to favour the usage of new materials and production processes. The present work introduces an integrated approach using Design of Experiment, FEA experimentation and RSM to study the effect of deep drawing process parameters and resulting defects associated with sheet metal forming processes such as thinning and earing particularly in deep drawing of non-circular parts. The Design of Experiment (DoE) is used to generate a series of experiments for different process parameters in deep drawn rectangular products which are then simulated …
Infrared Phase-Change Chiral Metasurfaces With Tunable Circular Dichroism, Haotian Tang, Liliana Stan, David A. Czaplewski, Xiaodong Yang, Jie Gao
Infrared Phase-Change Chiral Metasurfaces With Tunable Circular Dichroism, Haotian Tang, Liliana Stan, David A. Czaplewski, Xiaodong Yang, Jie Gao
Mechanical and Aerospace Engineering Faculty Research & Creative Works
Integrating Phase-Change Materials in Meta surfaces Has Emerged as a Powerful Strategy to Realize Optical Devices with Tunable Electromagnetic Responses. Here, Phase-Change Chiral Meta surfaces based on GST-225 Material with the Designed Trapezoid-Shaped Resonators Are Demonstrated to Achieve Tunable Circular Dichroism (CD) Responses in the Infrared Regime. the Asymmetric Trapezoid-Shaped Resonators Are Designed to Support Two Chiral Plasmonic Resonances with OppositeCDresponses for Realizing SwitchableCDbetween Negative and Positive Values using the GST Phase Change from Amorphous to Crystalline. the Electromagnetic Field Distributions of the Chiral Plasmonic Resonant Modes Are Analyzed to Understand the Chiroptical Responses of the Meta surface. Furthermore, the …
Assessing The Benefits Of Electrification For The Mackinac Island Ferry From An Environmental And Economic Perspective, Siddharth Gopujkar, Jeremy Worm
Assessing The Benefits Of Electrification For The Mackinac Island Ferry From An Environmental And Economic Perspective, Siddharth Gopujkar, Jeremy Worm
Michigan Tech Publications
Ferry electrification has gained attention in the last decade as a potential path to reduce greenhouse gas emissions. This study, conducted by APS LABS at Michigan Technological University for the Mackinac Economic Alliance (MEA) and funded by the Michigan Economic Development Corporation (MEDC), looked at the feasibility and potential benefits of electrification of a particular vessel that is part of a ferry service from Mackinaw City, Michigan, USA, to Mackinac Island, Michigan, USA. The study included a comprehensive analysis of the feasibility of retrofitting the current configuration of the ferry into an all-electric ferry based on the availability of components …
Estimation Of Useful-Stage Energy Returns On Investment For Fossil Fuels And Implications For Renewable Energy Systems, Emmanuel Aramendia, Paul E. Brockway, Peter G. Taylor, Jonathan B. Norman, Matthew K. Heun, Zeke Marshal;
Estimation Of Useful-Stage Energy Returns On Investment For Fossil Fuels And Implications For Renewable Energy Systems, Emmanuel Aramendia, Paul E. Brockway, Peter G. Taylor, Jonathan B. Norman, Matthew K. Heun, Zeke Marshal;
University Faculty Publications and Creative Works
The net energy implications of the energy transition have so far been analysed at best at the final energy stage. Here we argue that expanding the analysis to the useful stage is crucial. We estimate fossil fuelsʼ useful-stage energy returns on investment (EROIs) over the period 1971–2020, globally and nationally, and disaggregate EROIs by end use. We find that fossil fuelsʼ useful-stage EROIs (~3.5:1) are considerably lower than at the final stage (~8.5:1), due to low final-to-useful efficiencies. Further, we estimate the final-stage EROI for which electricity-yielding renewable energy would deliver the same net useful energy as fossil fuels (EROI …
A Multi-Material Platform For Imaging Of Single Cell-Cell Junctions Under Tensile Load Fabricated With Two-Photon Polymerization, Jordan Rosenbohm, Grayson Minnick, Bahareh Tajvidi Safa, Amir M. Esfahani, Xiaowei Jin, Haiwei Zhai, Nickolay V. Lavrik, Ruiguo Yang
A Multi-Material Platform For Imaging Of Single Cell-Cell Junctions Under Tensile Load Fabricated With Two-Photon Polymerization, Jordan Rosenbohm, Grayson Minnick, Bahareh Tajvidi Safa, Amir M. Esfahani, Xiaowei Jin, Haiwei Zhai, Nickolay V. Lavrik, Ruiguo Yang
Department of Mechanical and Materials Engineering: Faculty Publications
We previously reported a single-cell adhesion micro tensile tester (SCAμTT) fabricated from IP-S photoresin with two-photon polymerization (TPP) for investigating the mechanics of a single cell-cell junction under defined tensile loading. A major limitation of the platform is the autofluorescence of IP-S, the photoresin for TPP fabrication, which significantly increases background signal and makes fluorescent imaging of stretched cells difficult. In this study, we report the design and fabrication of a new SCAμTT platform that mitigates autofluorescence and demonstrate its capability in imaging a single cell pair as its mutual junction is stretched. By employing a two-material design using IP-S …