Mechanical Engineering Commons^™

Small Footprint High Flow Rate Microdevice For Rare Target Cell Capture, Taehyun Park

LSU Doctoral Dissertations

A novel high flow rate cell capture design was introduced to overcome the limitations of the current technologies or methods for rare target cell capture. Even though the rare target cell capture using BioMEMS technology has great potential for cancer diagnosis, previous rare cell capture research could not overcome the limitations of low flow rate or low recovery rate. Rare cell research requires precise sample preparation for accurate results. A new method of preparation for a single or a precise number of target cell was introduced. Current sample preparation methods which are not suitable for rare cell research, such as …

A Feasibility Study Of Model-Based Natural Ventilation Control In A Midrise Student Dormitory Building, Steven James Gross

Dissertations and Theses

Past research has shown that natural ventilation can be used to satisfy upwards of 98% of the yearly cooling demand when utilized in the appropriate climate zone. Yet widespread implementation of natural ventilation has been limited in practice. This delay in market adoption is mainly due to lack of effective and reliable control. Historically, control of natural ventilation was left to the occupant (i.e. they are responsible for opening and closing their windows) because occupants are more readily satisfied when given control of the indoor environment. This strategy has been shown to be effective during summer months, but can lead …

Initial Design, Manufacture, And Testing Of A Cubelab Module Frame For Biological Payloads Aboard The International Space Station, Twyman Samuel Clements

University of Kentucky Master's Theses

This thesis investigates the design of a CubeLab Module frame to facilitate biological research aboard the International Space Station (ISS). With the National Laboratory designation of the ISS by the United States Congress the barriers for use of the facility have been lowered for commercial and academic entities, allowing greater volume and diversity in the research that can be done. Researchers in biology and other areas could benefit from development and adoption of a plug-and-play payload containment system for use in the microgravity/space environment of the ISS. This research includes design and analysis of such a system. It also includes …

Analyses Of Variable Refrigerant Flow And Exergy In Air Conditioning Systems, Rashid Ali Alshatti

USF Tampa Graduate Theses and Dissertations

This thesis consists of two research problems in the air conditioning (A/C) area. For the first problem, the aim is to model and simulate a variable refrigerant flow (VRF) air conditioning system. The coefficient of performance (COP) for refrigeration or heat pump system is one of the critical parameters for designing an air conditioning system. The modeling of the system components for a VRF cycle under different cooling conditions using R-134a and R-22 as refrigerants was carried out. Calculations were performed by varying different parameters such as condenser and evaporator temperatures, and refrigerant type. The R-134a refrigerant shows a better …

Thermo-Mechanical Beam Element For Analyzing Stresses In Functionally Graded Materials, Simon Caraballo

USF Tampa Graduate Theses and Dissertations

Modeling at the structural scale most often requires the use of beam and shell elements. This simplification reduces modeling complexity and computation requirements but sacrifices the accuracy of through-the-thickness information. Several studies have reported various design approaches for analyzing functionally graded material structures. One of these studies proposed a two-node beam element for functionally graded materials (FGMs) based on first order shear deformable (FOSD) theory. The derivation of governing equations included spatial temperature variation. However, only the constant temperature case was carried through in the element formulation. This investigation explore the effects of spatial temperature variation in the axial and …

Theoretical And Experimental Analysis Of Power And Cooling Cogeneration Utilizing Low Temperature Heat Sources, Gökmen Demirkaya

USF Tampa Graduate Theses and Dissertations

Development of innovative thermodynamic cycles is important for the efficient utilization of low-temperature heat sources such as solar, geothermal, and waste heat sources. Binary mixtures exhibit variable boiling temperatures during the boiling process, which leads to a good thermal match between the heating fluid and working fluid for efficient heat source utilization. This study presents a theoretical and an experimental analysis of a combined power/cooling cycle, which combines the Rankine and absorption refrigeration cycles, uses ammonia-water mixture as the working fluid and produces power and refrigeration, while power is the primary goal. This cycle, also known as the Goswami Cycle, …

Steady And Transient Heat Transfer For Jet Impingement On Patterned Surfaces, Mark Michael Dobbertean

USF Tampa Graduate Theses and Dissertations

Free liquid-jet impingement is well researched due to its high heat transfer ability and ease of implementation. This study considers both the steady state and transient heating of a patterned plate under slot-free-liquid jet impingement. The primary working fluid was water (H2O) and the plate material considered was silicon. Calculations were done for Reynolds number (Re) ranging from 500 to 1000 and indentation depths from 0.000125 to 0.0005 m for three different surface configurations. The effect of using different plate materials and R-134a as the working fluid were explored for the rectangular step case. The distributions of the local and …

Regulation Of Cell Adhesion Strength By Spatial Organization Of Focal Adhesions, Kranthi Kumar Elineni

USF Tampa Graduate Theses and Dissertations

Cell adhesion to extracellular matrix (ECM) is critical to various cellular processes like cell spreading, migration, growth and apoptosis. At the tissue level, cell adhesion is important in the pathological and physiological processes that regulate the tissue morphogenesis. Cell adhesion to the ECM is primarily mediated by the integrin family of receptors. The receptors that are recruited to the surface are reinforced by structural and signaling proteins at the adhesive sites forming focal adhesions that connect the cytoskeleton to further stabilize the adhesions. The functional roles of these focal adhesions extend beyond stabilizing adhesions and transduce mechanical signals at the …

Polydimethylsiloxane Mechanical Properties Measured By Macroscopic Compression And Nanoindentation Techniques, Zhixin Wang

USF Tampa Graduate Theses and Dissertations

In this thesis, the relationship between the elastic modulus of PDMS and the base/agent ratio (the amount of crosslinking) is studied. Reliable macroscopic compression test instrument was developed. Preload method was applied for the nanoindentation flat punch test to develop full contact.

In chapter 2, an easy instrument setup for macroscopic compression test is described. A series of PDMS samples with different base/agent ratios were tested using the macroscopic compression method. The relationship between PDMS elastic modulus and its base/agent ratio was established.

In chapter 3, PDMS nanoindentation DMA tests provide stable data with different test control models. The storage …

Numerical Simulations Of Heat Transfer Processes In A Dehumidifying Wavy Fin And A Confined Liquid Jet Impingement On Various Surfaces, Mutasim Mohamed Sarour Elsheikh

USF Tampa Graduate Theses and Dissertations

This thesis consists of two different research problems. In the first one, the heat transfer characteristic of wavy fin assembly with dehumidification is carried out. In general, fin tube heat exchangers are employed in a wide variety of engineering applications, such as cooling coils for air conditioning, air pre-heaters in power plants and for heat dissipation from engine coolants in automobile radiators. In these heat exchangers, a heat transfer fluid such as water, oil, or refrigerant, flows through a parallel tube bank, while a second heat transfer fluid, such as air, is directed across the tubes. Since the principal resistance …

Interface Engineered Diamond Coatings For Dry Machining Applications, Humberto Arturo Gomez Vega

USF Tampa Graduate Theses and Dissertations

Several studies have been propose to improve the adhesion of diamond films on cemented carbide tool materials, however a systematic study in identifying the role of the factors that affect the final diamond adhesion and the resulting machining performance of the tool under real manufacturing conditions is still unexplored. CVD diamond film's extraordinary qualities bring little benefit if the film fails to adhere sufficiently to the substrate. Inadequate adhesion undermines tool performance and longevity, causing unpredictable behavior under load and possibly leading to unexpected failure of the tool in the production line. This dissertation investigates the effects of different surface …

Diamond Based-Materials: Synthesis, Characterization And Applications, Qiang Hu

USF Tampa Graduate Theses and Dissertations

The studies covered in this dissertation concentrate on the various forms of diamond films synthesized by chemical vapor deposition (CVD) method, including microwave CVD and hot filament CVD. According to crystallinity and grain size, a variety of diamond forms primarily including microcrystalline (most commonly referred to as polycrystalline) and nanocrystalline diamond films, diamond-like carbon (DLC) films were successfully synthesized. The as-grown diamond films were optimized by changing deposition pressure, volume of reactant gas hydrogen (H2) and carrier gas argon (Ar) in order to get high-quality diamond films with a smooth surface, low roughness, preferred growth orientation and high sp3 bond …

Self-Alignment Of Silicon Microparts On A Hexadecane-Water Interface By Surface Tension, Caroline Elizabeth Liberti

USF Tampa Graduate Theses and Dissertations

Mechanical assembly of systems and structures on the micro-scale can be inefficient as particles of sub-millimeter dimensions are difficult to manipulate. Cutting edge manufacturing methods implement self-assembly as an approach to ordering micro and nano-sized parts into a desired arrangement. This thesis studies a technique utilizing surface tension as a method of actuating microparts on a liquid-liquid interface via lateral capillary interactions. Preliminary experimentation is conducted to investigate the feasibility of developing a new method for self-alignment of microparts by observing the influence of interfacial geometry on the movement of silicon tiles along a hexadecanewater interface. Different surface geometries are …

Fatigue Testing And Computational Analysis Of A Spinal Implant, Timothy Helton

Electronic Theses and Dissertations

The following study investigates the fatigue behavior of a spinal implant developed by Synthes, Inc. to provide stability and off load pressure from the discs in the lumbar region. The installation process for the StenoFix design utilizes plastic deformations to customize the device to each individual patient, but also has potential to degrade fatigue behavior. Physical testing of two titanium alloys, Ti-6Al-7Nb and Ti-15Mo, was conducted on hydraulic test frames and compared to computational simulations which were carried out for the following scenarios: cyclic excitation following plastic deformation; variation of material property definition; variation of loading location; and three modifications …

Use Of Collaborative Learning Technology To Support Cross-Faculty Group Learning, Colm O'Kane, Jennifer Mcdonnell

Conference Papers

The scope of applications of computer supported collaborative learning (CSCL) has grown exponentially in recent years. This group of techniques has been found to be extremely effective as a collaborative tool to inspire scaffolding between students. It is also useful when several academic supervisors wish to view the same project work.

This paper describes the process and the motivation behind the first implementation of a campus pack-based project in Year 3 of Technological University Dublin's (DIT) BSc. in Product Design programme. Issues have been encountered in previous years with this cross-faculty managed project, but this work shows the potential that …

Study Of Turbulence-Radiation Interaction In Hypersonic Turbulent Boundary Layers, L. (Lian) Duan, A. M. Feldick, M. P. Martín, M. F. Modest, D. A. Levin

Mechanical and Aerospace Engineering Faculty Research & Creative Works

In the paper, we conduct direct numerical simulations (DNS) to investigate the effect of turbulence-radiation interaction (TRI) in hypersonic turbulent boundary layers, representative of the Orion crew exploration vehicle (CEV) at peak heating condition during reentry. The radiative transfer equation (RTE) is solved using the tangent slab approximation. 1 The RTE solver is line-by-line (LBL) accurate, making use of a developed efficient spectral database 2 for spectral modeling. A multi-group full-spectrum correlated-k-distribution (FSCK) method 3 is used to reduce the number of RTE evaluations while preserving LBL accuracy. A nondimensional governing parameter to measure the significance of TRI is proposed, …

Study Of Turbulence-Chemistry Interaction In Hypersonic Turbulent Boundary Layers, Lian Duan, M. Pino Martín

Mechanical and Aerospace Engineering Faculty Research & Creative Works

Studies of the turbulence-chemistry interaction (TCI) are performed in hypersonic turbulent boundary layers using direct numerical simulation (DNS) flow fields under typical hypersonic conditions representative of blunt-body and slender-body hypersonic vehicles, with super catalytic and noncatalytic wall conditions in pure air. Nondimensional governing parameters, 'interaction' Damk̈ohler number and relative heat release, are proposed to measure the influence of TCI on flow composition and temperature. Both a priori and posteriori studies are performed to assess the effect of TCI on chemical production rates and mean and turbulence flow characteristics. In addition, an effective approach (called PDF-TS) to estimate the intensity of …

Integrated Reliable And Robust Design, Gowrishankar Ravichandran

Masters Theses

"The objective of this research is to develop an integrated design methodology for reliability and robustness. Reliability-based design (RBD) and robust design (RD) are important to obtain optimal design characterized by low probability of failure and minimum performance variations respectively. But performing both RBD and RD in a product design may be conflicting and time consuming. An integrated design model is needed to achieve both reliability and robustness simultaneously. The purpose of this thesis is to integrate reliability and robustness. To achieve this objective, we first study the general relationship between reliability and robustness. Then we perform a numerical study …

Advanced Underwater Vehicle Navigation, M. Jordan Stanway

Link Foundation Ocean Engineering and Instrumentation Fellowship Reports

Underwater vehicles–both manned and unmanned–are critical in our efforts to explore and study the depths of the ocean. Most underwater sensors possess a relatively small field of view; this means they must be carried over large distances to achieve high spatial coverage and resolution. The vast amount of data these sensors collect is much more valuable if the location of the vehicle is precisely known at the time the data is collected. This gives rise to the maxim: The data is only as good as the navigation. Navigation remains a challenging problem for underwater vehicles. Fundamental limitations of sensing technology …

A Shock Tube Technique For Blast Wave Simulation And Studies Of Flow Structure Interactions In Shock Tube Blast Experiments, Nicholas N. Kleinschmit

Department of Engineering Mechanics: Dissertations, Theses, and Student Research

Improvised explosive devices (IED’s) are widely used against US and allied forces fighting in Iraq and Afghanistan. Exposure to IED blast may cause blast-induced traumatic brain injury (bTBI). The injury mechanisms are however not well understood. A critical need in bTBI-related research is the ability to replicate the loading conditions of IED blast waves in a laboratory environment. In this work, experimental studies have been carried out to explore the use of the shock tube technique for generating air shock waves that mimic the temporal and spatial characteristics of free-field blast waves and to investigate the blast wave-test sample interactions …

In Vitro Models For Glaucoma Research: Effects Of Hydrostatic Pressure, Yuan Lei, Shadi Rajabi, Ryan M. Pedrigi, Darryl R. Overby, A. Thomas Read, C. Ross Ethier

Department of Mechanical and Materials Engineering: Faculty Publications

PURPOSE. The response of cells (e.g., optic nerve head [ONH] cells) to mechanical stress is important in glaucoma. Studies have reported the biological effects of hydrostatic pressure on ONH cells cultured on a rigid substrate. An apparatus, designed to independently vary hydrostatic pressure and gas tension (including oxygen tension) in culture medium, was used to evaluate the effects of pressure and tension on cell migration, shape, and α-tubulin architecture in a transformed cell line (DITNC1 rat cortical astrocytes).

METHODS. During the assay period, cells were exposed to one of four experimental configurations: (1) control pressure and control gas tension; (2) …

Nanomechanical Analysis Of Insulinoma Cells After Glucose And Capsaicin Stimulation Using Atomic Force Microscopy, Ruiguo Yang, Ning Xi, King Wai-Chu Lai, Bei-Hua Zhong, Carmen Kar-Man Fung, Chen-Geng Qu, Donna H. Wang

Department of Mechanical and Materials Engineering: Faculty Publications

Aim: Glucose stimulates insulin secretion from pancreatic islet β cells by altering ion channel activity and membrane potential in the β cells. TRPV1 channel is expressed in the β cells and capsaicin induces insulin secretion similarly to glucose. This study aims to investigate the biophysical properties of the β cells upon stimulation of membrane channels using an atomic force microscopic (AFM) nanoindentation system.

Methods: ATCC insulinoma cell line was used. Cell stiffness, a marker of reorganization of cell membrane and cytoskeleton due to ion channel activation, was measured in real time using an integrated AFM nanoindentation system. Cell height that …

Dynamic Response Of Brain Subjected To Blast Loadings: Influence Of Frequency Ranges, Mehdi S. Chafi, Shailesh G. Ganpule, Linxia Gu, Namas Chandra

Department of Mechanical and Materials Engineering: Faculty Publications

Blast wave induced a frequency spectrum and large deformation of the brain tissue. In this study, new material parameters for the brain material are determined from the experimental data pertaining to these large strain amplitudes and wide frequencies ranging (from 0.01 Hz to 10 MHz) using genetic algorithms. Both hyperelastic and viscoelastic behavior of the brain are implemented into 2D finite element models and the dynamic responses of brain are evaluated. The head, composed of triple layers of the skull, including two cortical layers and a middle dipole sponge-like layer, the dura, cerebrospinal fluid (CSF), the pia mater and the …

Comparative Analysis Of The Biaxial Mechanical Behavior Of Carotid Wall Tissue And Biological And Synthetic Materials Used For Carotid Patch Angioplasty, Alexey Kamenskiy, Iraklis I. Pipinos, Jason N. Mactaggart, Syed A. Jaffar Kazmi, Yuris A. Dzenis

Department of Mechanical and Materials Engineering: Faculty Publications

Patch angioplasty is the most common technique used for the performance of carotid endarterectomy. A large number of patching materials are available for use while new materials are being continuously developed. Surprisingly little is known about the mechanical properties of these materials and how these properties compare with those of the carotid artery wall. Mismatch of the mechanical properties can produce mechanical and hemodynamic effects that may compromise the long-term patency of the endarterectomized arterial segment. The aim of this paper was to systematically evaluate and compare the biaxial mechanical behavior of the most commonly used patching materials. We compared …

It’S Not What You Think: A Theory For Understanding The Lack Of Interest Among Domestic Students In The Engineering Phd, Michelle C. Howell Smith, Amanda L. Garrett, Ellen Weissinger, Namas Chandra

Department of Mechanical and Materials Engineering: Faculty Publications

We live in a fast-paced world surrounded by technological advances. Engineers with advanced skills perform important functions in our society. However we know very little about how engineers consider obtaining advanced education and skills. The purpose of this study is to understand and develop a theory explaining the process domestic engineers undergo in developing an interest in obtaining a PhD in engineering. Our research was guided by the following central research question: What is the theory that explains the process of developing interest in doctoral-level engineering education for engineers? We used qualitative, grounded theory methods, to investigate the process of …

Frequency Shifts In Plate Crystal Resonators Induced By Electric, Magnetic, Or Mechanical Fields In Surface Films, Nan Liu, J.S. Yang, Yuantai Hu, Xuedong Chen, Wei Jiang

Department of Mechanical and Materials Engineering: Faculty Publications

We study frequency shifts in plate crystal resonators with surface films. The films are multiphysical, including the effects of inertia, stiffness, intrinsic stress, piezoelectric coupling, and piezomagnetic coupling. Mindlin’s two-dimensional equations for a crystal plate with two elastic surface films are generalized to include the multiphysical effects of the films. They are used to study thickness-shear vibrations of a rotated Y-cut quartz plate with initial fields resulting from the mechanical, electric, and magnetic fields in the surface films. Frequency shifts caused by the initial fields are calculated and examined. Results show that plate crystal resonators with multiphysical surface films may …

A Few Transient Effects In At-Cut Quartz Thickness-Shear Resonators, Runyu Zhang, Hongping Hu

Department of Mechanical and Materials Engineering: Faculty Publications

We study a few transient effects of AT-cut quartz thickness-shear resonators, including resonator turning on and turning off as well as voltage amplitude and frequency fluctuations. Mindlin’s two-dimensional plate equations are used and solved analytically. Both a sudden change and a gradual change of the driving voltage are studied. It is found that for a resonator with a frequency of 1.649430868 MHz and material quality factor of Q = 10⁵, the characteristic time scale of the transient effects is of the order of 0.1 s.

Use Of Environmental Scanning Electron Microscopy For In Situ Observation Of Interaction Of Cells With Micro- And Nanoprobes, Alexander Goponenko, B. J. Boyle, K. I. Jahan, Maxim V. Gerashchenko, Dmitri E. Fomenko, Vadim N. Gladyshev, Yuris A. Dzenis

Department of Mechanical and Materials Engineering: Faculty Publications

Precision intracellular sensing, probing and manipulation offer unprecedented opportunities for advances in biological sciences. Next-generation ultra-fine probes will be capable of targeting individual cell organelles. Development of such probes as well as probes capable of penetrating through tough cell walls requires detailed knowledge of cell-probe interaction. This Letter evaluated the applicability of environmental scanning electron microscopy (ESEM) for cell and cell-probe interaction imaging. Several types of cells (plant and yeast cells as well as mouse spermatozoa) were successfully imaged in their natural state, with mouse spermatozoa observed by ESEM for the first time. Computerized stage applied to image was tough …

Achieving High Efficiency Laminated Polymer Solar Cell With Interfacial Modified Metallic Electrode And Pressure Induced Crystallization, Yongbo Yuan, Yu Bi, Jinsong Huang

Department of Mechanical and Materials Engineering: Faculty Publications

We report efficient laminated organic photovoltaic device with efficiency approach the optimized device by regular method based on Poly(3-hexylthiophene-2,5-diyl) and [6,6]-phenyl-C₆₁-butyric acid methyl ester (PCBM). The high efficiency is mainly attributed to the formation of a concrete polymer/metal interface mechanically and electrically by the use of electronic-glue, and using the highly conductive and flexible silver film as anode to reduce photovoltage loss and modifying its work function for efficiency hole extraction by ultraviolet/ozone treatment, and the pressure induced crystallization of PCBM.

An Investigation Of The Reynolds Number Dependence Of The Near-Wall Peak In Canonical Wall Bounded Turbulent Channel Flow, Bahareh Estejab

University of Kentucky Master's Theses

An experimental investigation into fully developed high aspect ratio channels was undertaken. A review of the literature reveals that there is a need for accurate measurement of the inner peak value of streamwise turbulence intensity despite the large number of studies already completed. The scattered data on this subject could be attributed either to insufficient channel size (aspect ratio or length) or to hot-wire spatial filtering.

A new, high quality, channel flow facility was designed and constructed, considering the most recent geometric limitation provided in the literature. To obtain accurate results, data were acquired using hot-wire probes with constant viscous-scale …