Biomedical Commons^™

Inkjet Bioprinting Of Solid Peroxides For Constructing Oxygen Generating Scaffolds To Improve Cells Viability And Growth Under Hypoxic Environment, Daniel Reyna Soriano

Open Access Theses & Dissertations

Tissue engineering has emerged as an interdisciplinary field to overcome current challenges for tissue repair or replacement in the human body. In essence, it proposes new medical therapies customized to match the biology of specific patients. The potential benefits offered by tissue engineering have driven scientific inquiry to make it a clinical reality.

One of the current challenges in tissue engineering is to provide oxygen supply to thick tissues as oxygen diffusion is limited to 100 - 200 µm layer of viable tissue. Engineered vascular conduits are applied in vitro, providing pre-vascularization to tissues; however, host anastomosis is still a …

Bioprinting Oxygen Releasing Microparticles For Improved Cell Tissue Viability, Daniel Reyna

Open Access Theses & Dissertations

A major challenge in regenerative medicine/tissue engineering is the difficulty of providing adequate oxygen to all cells within implanted engineered tissues before full vascularization is achieved. To overcome this limitation, a variety of oxygen-releasing particles have been developed to improve cell and tissue survival. However, most of these particles are used in random mixtures within scaffolding materials, which usually leads to an uneven distribution of oxygen in bioengineered tissues. An ideal oxygen supply requires a precise depositing control of oxygen-releasing particles in scaffolds. Cell bioprinting is a novel tissue fabrication approach, in which the bio-printer can be programmed to deposit …

Scaffold Based In-Vitro Osteodistraction For Bone Tissue Engineering, Cesar Adrian Terrazas

Open Access Theses & Dissertations

Engineering of hard tissues such as bone is a challenge because of the limited ability of the body to heal large defects. Current treatment options, such as employing bone sections from the patient or a deceased donor have been utilized for some time now with various degrees of success. However, these methods still exhibit many limitations such as limited supply and the possibility of transmission of disease.

To address the many limitations of bone grafts, synthetic engineered substitutes have been developed and widely researched for nearly three decades. Generally termed scaffolds, these are matrices that serve the purpose of temporary …

Synthesis And Manufacture Of Photocrosslinkable Poly(Caprolactone)-Based 3d Scaffolds For Tissue Engineering Applications, Nathan Jonathan Castro

Open Access Theses & Dissertations

It is known that the body can efficiently repair hard tissue (bone) micro fractures by suturing the defect through the deposition of minerals resulting in an area that is stronger post-injury. Larger defects, however, generally cause more trouble since the body is incapable of repairing them. Bone defects can occur as a result of congenital abnormalities, trauma, or disease. Traditional methods for addressing these defects have involved the use of acellular cadaverous bone or autologous bone. Both contain innate problems associated with them; the former method can result in disease transmission, as well as very low integration with the host …

Exploring Poly(Ethylene Glycol) As A Suitable Material For Peripheral Nerve Regeneration Scaffolds Manufactured By Stereolithography, Nubia Zuverza

Open Access Theses & Dissertations

One of the challenges in tissue engineering is to have spatial and temporal control over the biological elements within a scaffold used to guide regeneration for example of transected nerves. Some of the physical and chemical characteristics to regulate include incorporation of bioactive domains and release of chemical signals. This study presents the use of stereolithography (SL) to incorporate localized domains for cell adhesion in addition to include releasable nerve growth factor (NGF) in the process of building poly(ethylene glycol)diacrylate (PEGda) hydrogel scaffolds. Besides providing sites for cell attachment, an ideal nerve guidance conduit (NGC) should be able to release …