Biomedical Commons^™

Stem Cell Induction Via Inkjet-Mediated Gene Transfection, Gilbert Paquian Jr.

Open Access Theses & Dissertations

In 2009 and 2011, it was shown that micropores in the cell membrane that form during inkjet cell printing are a potential vector for gene transfection1,2. These papers showed that porcine aortic endothelial cells and Chinese hamster ovary cells were coprinted with green fluorescent protein-coding (GFP) plasmids onto collagen or gelatin "biopaper" substrates. Printed cells showed a viability of ~90% with transfection efficiencies ranging from 2-30%1,2. Using inkjet-mediated gene transfection (IMGT) to insert a plasmid coding for the Yamanaka stem cell induction factors, it may be possible to offset common drawbacks of the induction process, such as low efficiency, throughput, …

A Software Application For Cardiac-Gated Computerized Tomography Scanning, Stephen Caldwell, Trevor Engelsman

Williams Honors College, Honors Research Projects

Computerized tomography (CT) scans are a common clinical imaging procedure used worldwide. Operating in the X-Ray spectrum, these machines rotate scanners around a stationary body in order to compile two-dimensional images into a unified three-dimensional image. With adjustment to scan frequency and intensity, internal features such as muscles, organs, and tendons can easily be viewed. However, the heart has long evaded CT use due to its near-constant motion. Recently, cardiac-gated scans have entered the market as a technique to image the heart at a specific moment in time when it is nearly still - the quiescent period. Occurring during rapid …

Computational Tracking Of Shear-Mediated Platelet Interactions With Von Willebrand Factor, Adam Ralph, Martin Somers, Jonathan Cowman, Bruno Voisin, Emma Hogan, Hannah Dunne, Eimear Dunne, Barry Byrne, Nigel Kent, Antonio J. Ricco, Dermot Kenny, Simon Wong

Articles

The imaging of shear-mediated dynamic platelet behavior interacting with surface-immobilized von Willebrand factor (vWF) has tremendous potential in characterizing changes in platelet function for clinical diagnostics purposes. However, the imaging output, a series of images representing platelets adhering and rolling on the surface, poses unique, non-trivial challenges for software algorithms that reconstruct the positional trajectories of platelets. We report on an algorithm that tracks platelets using the output of such flow run experiments, taking into account common artifacts encountered by previously-published methods, and we derive seven key metrics of platelet dynamics that can be used to characterize platelet function. Extensive …

Hardware Design And Integration And Software Programming For Optical Elastography, Nima Taherkhani

Dissertations, Master's Theses and Master's Reports

The elastic and viscoelastic properties of skin are a reflection of its health. Measurement of these elastic properties can be used as the indicator of various pathologies. The measurement concept in our works is to mechanically stimulate the skin with a low frequency pneumatic force using non-invasive means of deflecting the skin and use laser speckle to study the elasticity of the skin.

The goal of this work was to develop a setup for in vivo optical elastography of skin. This setup includes an electro-mechanical device which is able to deliver a sinusoidal pneumatic stimulus equivalent to 10 grams of …

Detecting, Segmenting And Tracking Bio-Medical Objects, Mingzhong Li

Doctoral Dissertations

"Studying the behavior patterns of biomedical objects helps scientists understand the underlying mechanisms. With computer vision techniques, automated monitoring can be implemented for efficient and effective analysis in biomedical studies. Promising applications have been carried out in various research topics, including insect group monitoring, malignant cell detection and segmentation, human organ segmentation and nano-particle tracking.

In general, applications of computer vision techniques in monitoring biomedical objects include the following stages: detection, segmentation and tracking. Challenges in each stage will potentially lead to unsatisfactory results of automated monitoring. These challenges include different foreground-background contrast, fast motion blur, clutter, object overlap and …

The Viability Of High-Frequency Oscillation Analysis In Eeg Signals For Seizure Prediction, Bryan David Kern

Open Access Theses & Dissertations

Seizure prediction is a decades-old research problem that has yet to reach any satisfying conclusions. Many studies have tackled the problem with varying results, but there has yet to be any major breakthroughs that define the direction of all future research. However, a promising, new predictor may have presented itself in recent years in the form of high frequency oscillations (HFOs). With the discovery of HFOs (80 - 800 Hz) as a biomarker for epilepsy, new interest has been placed in studying the high frequency content of EEGs to find a possible link between epilepsy and HFOs. In this paper, …

Wettability Of Magnesium Based Alloys, Victor Manuel Ornelas

Open Access Theses & Dissertations

The premise of this project was to determine the wettability behavior of Mg-based alloys using three different liquids. Contact angle measurements were carried out along with utilizing the Zisman method for obtaining values for the critical surface tension. Adhesion energy values were also found through the use of the Young-Dupre equation. This project utilized the Mg-based alloy Mg-2Zn-2Gd with supplemented α-Minimum Essential Medium (MEM), Phosphate Buffer Saline solution (PBS), and distilled water. These three liquids are commonly used in cell cultivation and protein adsorption studies. Supplemented α-MEM consisted of α-MEM, fetal bovine serum, and penicillin-streptomycin. Mg-2Zn-2Gd was used because of …

In-Shoe Plantar Pressure System To Investigate Ground Reaction Force Using Android Platform, Ahmed A. Mostfa

Theses and Dissertations

Human footwear is not yet designed to optimally relieve pressure on the heel of the foot. Proper foot pressure assessment requires personal training and measurements by specialized machinery. This research aims to investigate and hypothesize about Preferred Transition Speed (PTS) and to classify the gait phase of explicit variances in walking patterns between different subjects. An in-shoe wearable pressure system using Android application was developed to investigate walking patterns and collect data on Activities of Daily Living (ADL). In-shoe circuitry used Flexi-Force A201 sensors placed at three major areas: heel contact, 1st metatarsal, and 5th metatarsal with a PIC16F688 microcontroller …