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2014

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Articles 271 - 300 of 314

Full-Text Articles in Other Civil and Environmental Engineering

Multiscale Spatial Sensitivity Analysis For Agent-Based Modelling Of Coupled Landscape And Aquatic Systems, Arika Ligmann-Zielinska, Wei Liu, Daniel B. Kramer, Kendra Spence Cheruvelil, Patricia A. Soranno, Piotr Jankowski, Seda Salap Jun 2014

Multiscale Spatial Sensitivity Analysis For Agent-Based Modelling Of Coupled Landscape And Aquatic Systems, Arika Ligmann-Zielinska, Wei Liu, Daniel B. Kramer, Kendra Spence Cheruvelil, Patricia A. Soranno, Piotr Jankowski, Seda Salap

International Congress on Environmental Modelling and Software

Models of coupled landscape and aquatic systems (CLAS) are prone to input uncertainties that vary over space. To address this challenge, we employ a comprehensive model evaluation that: [1] quantifies the variability of model results (uncertainty analysis), and [2] decomposes this variability based on the relative contribution of inputs to identify major drivers in the model (sensitivity analysis). Our study simulates how agricultural land conversion from active to fallow lands reduces nutrient loading to lakes. We employ an agent-based model of farmer decision making coupled with a spatially-explicit biophysical lake model. A number of model inputs are uncertain including: variables …

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Defining The Spatiotemporal Surveillance Space For Alien Species' Invasions Using Approximate Bayesian Computation, Grant Hamilton, Rune Rasmussen, Jana Mullerova, Jan Pergl, Petr Pysek Jun 2014

Defining The Spatiotemporal Surveillance Space For Alien Species' Invasions Using Approximate Bayesian Computation, Grant Hamilton, Rune Rasmussen, Jana Mullerova, Jan Pergl, Petr Pysek

International Congress on Environmental Modelling and Software

The spatiotemporal dynamics of an alien species invasion across a real landscape are typically complex. While surveillance is an essential part of a management response, planning surveillance in space and time present a difficult challenge due to this complexity. We show here a method for determining the highest probability sites for occupancy across a landscape at an arbitrary point in the future, based on occupancy data from a single slice in time. We apply to the method to the invasion of Giant Hogweed, a serious weed in the Czech republic and throughout Europe.

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The Effect Of Recovery On Modeling Inactivation Of Bacillus Spores On Hvac Filters, Bharathi Murali, Jade Mitchell Jun 2014

The Effect Of Recovery On Modeling Inactivation Of Bacillus Spores On Hvac Filters, Bharathi Murali, Jade Mitchell

International Congress on Environmental Modelling and Software

The ability of microorganisms to persist on fomite surfaces is an important component in modeling their spread in physical environment. For example, Bacillus anthracis (Ba) spores have been found to be extremely resistant to inactivation, environmental stresses, and stable over decades. Modeling the inactivation of spores could form an integral element for estimating the exposure and the subsequent health risks posed by them. However, there is a knowledge gap in the quantification of recovery of Bacillus spores on porous surfaces, which may have a significant effect on the quantification of their persistence in the environment. Our work investigates the recovery …

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A K-Means Clustering Approach To Assess Wheat Yield Prediction Uncertainty With A Hydrus-1d Coupled Crop Model, Derek Groenendyk, Kelly Thorp, Ty Ferré, Wade Crow, Doug Hunsaker Jun 2014

A K-Means Clustering Approach To Assess Wheat Yield Prediction Uncertainty With A Hydrus-1d Coupled Crop Model, Derek Groenendyk, Kelly Thorp, Ty Ferré, Wade Crow, Doug Hunsaker

International Congress on Environmental Modelling and Software

Soil moisture, especially under drought conditions, is a factor that is known to impact crop yield predictions. Crop growth models used to make these predictions rely on soil texture estimates, which influence simulated soil moisture and ultimately crop growth. The purpose of this research was to implement a k-means clustering approach to address the uncertainty of the soil texture estimates. By grouping similar soil textures based on their simulated responses, clustering reveals how soil texture uncertainty may impact yield estimates. Wheat growth simulations were conducted using a HYDRUS 1D and coupled crop model for soils defined on the USDA soil …

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Gis-Based Environmental Modeling With Tangible Interaction And Dynamic Visualization, Anna Petrasova, Brendan Harmon, Vaclav Petras, Helena Mitasova Jun 2014

Gis-Based Environmental Modeling With Tangible Interaction And Dynamic Visualization, Anna Petrasova, Brendan Harmon, Vaclav Petras, Helena Mitasova

International Congress on Environmental Modelling and Software

We present a new, affordable version of TanGeoMS, a tangible geospatial modeling and visualization system designed for collaboratively exploring how terrain change impacts landscape pro­ cesses. It couples a physical, three-dimensional model of a landscape with geospatial modeling and analysis through a cycle of scanning and projection. Multiple users can modify the physical model by hand while it is being scanned; by sculpting the model they generate input for modeling of geophysical processes. The modeling results are then visualized by projecting images or animations back on the physical model. This feedback loop is an intuitive way to evaluate the impacts …

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Calibration Of Simulation Platforms Including Highly Interweaved Processes: The Maelia Multi-Agent Platform, Romain Lardy, Pierre Mazzega, Christophe Sibertin-Blanc, Yves Auda, José-Miguel Sanchez-Perez, Sabine Sauvage, Olivier Therond Jun 2014

Calibration Of Simulation Platforms Including Highly Interweaved Processes: The Maelia Multi-Agent Platform, Romain Lardy, Pierre Mazzega, Christophe Sibertin-Blanc, Yves Auda, José-Miguel Sanchez-Perez, Sabine Sauvage, Olivier Therond

International Congress on Environmental Modelling and Software

The MAELIA project develops an agent-based modeling and simulation platform to study the environmental, economic and social impacts of various regulations regarding water use and water management in combination with climate change. An integrated modelling approach has been used to model the investigated social-ecological system. MAELIA combines spatiotemporal models of ecologic (e.g. water flow and plant growth) and human decision-making processes (e.g. cropping plan), socio-economic dynamics (e.g. land cover changes). Due to the diversity and the interweaving of the processes considered, the calibration and evaluation of such a multi-agent platform is a scientific challenge. Indeed, many parameters can reveal to …

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An Environmental Modeling Language For Agents And Fields, Kor De Jong, Merijn De Bakker, Derek Karssenberg Jun 2014

An Environmental Modeling Language For Agents And Fields, Kor De Jong, Merijn De Bakker, Derek Karssenberg

International Congress on Environmental Modelling and Software

Environmental modeling involves manipulating environmental attributes represented in soft­ware by agents, fields or both, but most modeling environments are designed to be especially useful for either agent-based or field-based modeling. Agent-based and field-based modeling environments have different properties with respect to their ease of use and how well both agents and fields can be represented and manipulated. Most agent-based modeling environments require the modeler to use a general purpose object oriented programming language like Java to express models, while field-based modeling environments often implement a high level, domain specific imperative language based on map algebra, or extent a general purpose …

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An Integrated Approach For Including Social Capacities, And Economic Valuation In Risk Assessment Of Water Related Hazards In Uncertain Scenarios, Carlo Giupponi, Vahid Mojtahed, Animesh K. Gain, Stefano Balbi, Claudio Biscaro Jun 2014

An Integrated Approach For Including Social Capacities, And Economic Valuation In Risk Assessment Of Water Related Hazards In Uncertain Scenarios, Carlo Giupponi, Vahid Mojtahed, Animesh K. Gain, Stefano Balbi, Claudio Biscaro

International Congress on Environmental Modelling and Software

We propose a conceptual framework, KR-FWK (i.e. KULTURisk Framework from the name of the European project within which it originated) and its implementation methods SERRA (Socio-Economic Regional Risk Assessment) for integrated (physical and economical) risk assessment, and economic valuation of risk prevention benefits on multiple receptors. The KR-FWK and the SERRA approach are characterised by: (i) integration of physical-environmental dimensions and the socio-economic ones in risk assessment; (ii) consideration of the role of social capacities (adaptive and coping capacity) in reducing risk and related costs, (iii) quantitative (even monetary) assessment of risks and of the benefits of risk reduction measures, …

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Agile Workflows For Climate Impact Risk Assessment Based On The Ci:Grasp Platform And The Jabc Modeling Framework, Samih Al-Areqi, Anna-Lena Lamprecht, Tiziana Margaria, Steffen Kriewald, Dominik Reusser, Markus Wrobel Jun 2014

Agile Workflows For Climate Impact Risk Assessment Based On The Ci:Grasp Platform And The Jabc Modeling Framework, Samih Al-Areqi, Anna-Lena Lamprecht, Tiziana Margaria, Steffen Kriewald, Dominik Reusser, Markus Wrobel

International Congress on Environmental Modelling and Software

Analyzing and assessing potential impacts of climate change are critical and challenging tasks that require the processing of large and heterogeneous datasets. These analyses are particularly demanding because of the multi-scale and multi-objective nature of environmental modeling for climate change impact assessment. The Climate Impacts: Global and Regional Adaptation Support Platform (ci:grasp) is a web-based climate information service for exploring climate change related information in its geographical context. In this paper, we show how the agile workflow development style supported by the jABC process modeling and execution framework permits us to leverage the processes implemented in the ci:grasp platform to …

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The Data Processing Inequality And Environmental Model Prediction, Steven V. Weijs Jun 2014

The Data Processing Inequality And Environmental Model Prediction, Steven V. Weijs

International Congress on Environmental Modelling and Software

Prediction in environmental systems, such as hydrological streamflow prediction, is a challenging task. Although on a small scale, many of the physical processes are well described, accurate predictions of macroscopical (e.g. catchment scale) behavior with a bottom-up mechanistic approach often remains elusive. On the other hand, conceptual or purely statistical models fitted to data often perform surprisingly well for prediction. The data processing inequality, from the field of information theory, says that processing data with statistical procedures can only decrease, and not increase the information content of the data. This seems to contradict the intuition that our knowledge of physical …

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Managing Agricultural Landscapes For Favouring Ecosystem Services Provided By Biodiversity: A Spatially Explicit Model Of Crop Rotations In The Gama Simulation Platform, Hugo Thierry, Aude Vialatte, Jean-Philippe Choisis, Benoit Gaudou, Claude Monteil Jun 2014

Managing Agricultural Landscapes For Favouring Ecosystem Services Provided By Biodiversity: A Spatially Explicit Model Of Crop Rotations In The Gama Simulation Platform, Hugo Thierry, Aude Vialatte, Jean-Philippe Choisis, Benoit Gaudou, Claude Monteil

International Congress on Environmental Modelling and Software

The need to reduce the use of pesticides in agricultural ecosystems requires studying and developing new pest regulation methods. Individual-based models are useful to better understand how dynamics of insects interact with agricultural landscapes and to study the potential effects of alternative regulation methods, taking into account the spatial and temporal stochasticity of agricultural ecosystems caused by human management. Crop rotations and crop phenology have an important impact on the life cycles of populations of insects. We developed an individual-based model simulating the dynamics of an agricultural landscape from GIS data. The spatiotemporal stochasticity is simulated using typical landcover rotations …

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Quantifying The Impacts Of Initial Condition And Model Uncertainty On Hydrological Forecasts, Caleb Matthew Dechant May 2014

Quantifying The Impacts Of Initial Condition And Model Uncertainty On Hydrological Forecasts, Caleb Matthew Dechant

Dissertations and Theses

Forecasts of hydrological information are vital for many of society's functions. Availability of water is a requirement for any civilization, and this necessitates quantitative estimates of water for effective resource management. The research in this dissertation will focus on the forecasting of hydrological quantities, with emphasis on times of anomalously low water availability, commonly referred to as droughts. Of particular focus is the quantification of uncertainty in hydrological forecasts, and the factors that affect that uncertainty. With this focus, Bayesian methods, including ensemble data assimilation and multi-model combinations, are utilized to develop a probabilistic forecasting system. This system is applied …

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A 3-D Hydrodynamic Modeling At Head Of Passes Of The Mississippi River, Tatiana Pavlyukova May 2014

A 3-D Hydrodynamic Modeling At Head Of Passes Of The Mississippi River, Tatiana Pavlyukova

LSU New Orleans Theses and Dissertations

A 3-D numerical model of the Head of Passes and Bird’s Foot Delta of the Mississippi River- was developed. The model was based on Delft3D and simulates the hydrodynamics and salinity transport for Head of Passes area from RM 6.5 to Gulf of Mexico. The model was calibrated, validated, and used to predict the response of the river to certain stimuli, such as - channel closures, channel modifications and diversions. The model includes West Bay, Southwest Pass, South Pass, Pass-A-Loutre and Main Pass. Three basic cases were developed: existing conditions, closure of Southwest Pass with a levee of 1.5m with …

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Damage Mapping Of Powdery Mildew In Winter Wheat With High-Resolution Satellite Image, Lin Yuan, Jingcheng Zhang, Yeyin Shi, Chenwei Nie, Liguang Wei, Jihua Wang Apr 2014

Damage Mapping Of Powdery Mildew In Winter Wheat With High-Resolution Satellite Image, Lin Yuan, Jingcheng Zhang, Yeyin Shi, Chenwei Nie, Liguang Wei, Jihua Wang

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Powdery mildew, caused by the fungus Blumeria graminis, is a major winter wheat disease in China. Accurate delineation of powdery mildew infestations is necessary for site-specific disease management. In this study, high-resolution multispectral imagery of a 25 km2 typical outbreak site in Shaanxi, China, taken by a newly-launched satellite, SPOT-6, was analyzed for mapping powdery mildew disease. Two regions with high representation were selected for conducting a field survey of powdery mildew. Three supervised classification methods—artificial neural network, mahalanobis distance, and maximum likelihood classifier—were implemented and compared for their performance on disease detection. The accuracy assessment showed that the ANN …

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Integrating Mammalian Hazards With Management At U.S. Civil Airports: A Case Study., Kristin M. Biondi, Jerrold L. Belant, James A. Martin, Travis L. Devault, Guiming Wang Apr 2014

Integrating Mammalian Hazards With Management At U.S. Civil Airports: A Case Study., Kristin M. Biondi, Jerrold L. Belant, James A. Martin, Travis L. Devault, Guiming Wang

United States Department of Agriculture Wildlife Services: Staff Publications

Wildlife incidents with U.S. civil aircraft cost an estimated $1.4 billion from 1990 to 2010, with mammals 5 times more likely to cause damage than other wildlife. We surveyed 2 general aviation (GA) airports and 6 Part-139 certificated (i.e., certified) airports to assess efficacy of management practices for mammalian species hazardous to aircraft. We obtained information on mammalian species present on airport grounds, types and estimated effectiveness of management techniques, and effort spent on wildlife management. We evaluated management techniques relative to aircraft–wildlife collisions (i.e., incident) frequencies taken from Federal Aviation Administration’s (FAA) National Wildlife Strike Database and species hazard …

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An Analysis Of Bicycle-Vehicle Interactions At Signalized Intersections With Bicycle Boxes, William Robert Farley Mar 2014

An Analysis Of Bicycle-Vehicle Interactions At Signalized Intersections With Bicycle Boxes, William Robert Farley

Dissertations and Theses

A before-and-after analysis was performed at eleven intersections where a bike box was installed in Portland, Oregon to explore the safety effects of the treatment. Video data were gathered prior to installation at 14 intersections where a bike box installation was planned by the Portland Bureau of Transportation. Cameras were set up to capture three full twenty-four hour days (72 hours) of data for each intersection from Tuesday through Thursday. Of the 14 original selected intersections, 11 intersections actually received the bike box treatment. Video data were again gathered for these intersections after the installation of the bike box for …

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2014 Directory Of Indiana State, County, City And Town Officials Responsible For Road And Street Work, Indiana Ltap Mar 2014

2014 Directory Of Indiana State, County, City And Town Officials Responsible For Road And Street Work, Indiana Ltap

Indiana Local Technical Assistance Program (LTAP) Directory

Contact information for local agency officials and employees responsible for Indiana’s local road infrastructure.

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Identification Of Wheat Varieties With A Parallel-Plate Capacitance Sensor Using Fisher’S Linear Discriminant Analysis, C. V. K. Kandala, George Meyer, N. Puppala, V. Settaluri, R. S. Reddy Feb 2014

Identification Of Wheat Varieties With A Parallel-Plate Capacitance Sensor Using Fisher’S Linear Discriminant Analysis, C. V. K. Kandala, George Meyer, N. Puppala, V. Settaluri, R. S. Reddy

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Fisher’s linear discriminant (FLD) models for wheat variety classification were developed and validated. The inputs to the FLD models were the capacitance (𝐶), impedance (𝑍), and phase angle (𝜃), measured at two frequencies. Classification of wheat varieties was obtained as output of the FLDmodels.𝑍and 𝜃of a parallel-plate capacitance system,holding thewheat samples, weremeasured using an impedance meter, and the 𝐶 value was computed.The best model developed classified the wheat varieties, with accuracy of 95.4%, over the six wheat varieties tested.This method is simple, rapid, and nondestructive and would be useful for the breeders and the peanut industry.

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Experimental Tools To Study Molecular Recognition Within The Nanoparticle Corona, Markita P. Landry, Sebastian Kruss, Justin T. Nelson, Gili Bisker, Nicole M. Iverson, Nigel F. Reuel, Michael S. Strano Jan 2014

Experimental Tools To Study Molecular Recognition Within The Nanoparticle Corona, Markita P. Landry, Sebastian Kruss, Justin T. Nelson, Gili Bisker, Nicole M. Iverson, Nigel F. Reuel, Michael S. Strano

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Advancements in optical nanosensor development have enabled the design of sensors using synthetic molecular recognition elements through a recently developed method called Corona Phase Molecular Recognition (CoPhMoRe). The synthetic sensors resulting from these design principles are highly selective for specific analytes, and demonstrate remarkable stability for use under a variety of conditions. An essential element of nanosensor development hinges on the ability to understand the interface between nanoparticles and the associated corona phase surrounding the nanosensor, an environment outside of the range of traditional characterization tools, such as NMR. This review discusses the need for new strategies and instrumentation to …

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Controlled De-Cross-Linking And Disentanglement Of Feather Keratin For Fiber Preparation Via A Novel Process, Helan Xu, Yiqi Yang Jan 2014

Controlled De-Cross-Linking And Disentanglement Of Feather Keratin For Fiber Preparation Via A Novel Process, Helan Xu, Yiqi Yang

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Pure protein fibers were fabricated from chicken feathers via a potentially green process. In the last several decades, efforts have been made to produce keratin-based industrial products, especially fibers. However, the methods of producing keratin fibers directly from chicken feathers could not be repeated. In this research, protein fibers from chicken feathers were developed using chemicals that could be either derived from renewable resources or facilely recycled. Backbones of keratin were preserved after cleavage of disulfide bonds using cysteine. Sodium dodecyl sulfate (SDS) was applied to dissolve keratin for spinning. Increasing SDS concentration intensified the ordered conformation of keratin, first …

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Water-Stable Three-Dimensional Ultrafine Fibrous Scaffolds From Keratin For Cartilage Tissue Engineering, Helan Xu, Shaobo Cai, Lan Xu, Yiqi Yang Jan 2014

Water-Stable Three-Dimensional Ultrafine Fibrous Scaffolds From Keratin For Cartilage Tissue Engineering, Helan Xu, Shaobo Cai, Lan Xu, Yiqi Yang

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Intrinsically water-stable scaffolds composed of ultrafine keratin fibers oriented randomly and evenly in three dimensions were electrospun for cartilage tissue engineering. Keratin has been recognized as a biomaterial that could substantially support the growth and development of multiple cell lines. Besides, three-dimensional (3D) ultrafine fibrous structures were preferred in tissue engineering due to their structural similarity to native extracellular matrices in soft tissues. Recently, we have developed a nontraditional approach to developing 3D fibrous scaffolds from alcohol-soluble corn protein, zein, and verified their structural advantages in tissue engineering. However, keratin with highly cross-linked molecular structures could not be readily dissolved …

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Intrinsically Water-Stable Keratin Nanoparticles And Their In Vivo Biodistribution For Targeted Delivery, Helan Xu, Zhen Shi, Narendra Reddy, Yiqi Yang Jan 2014

Intrinsically Water-Stable Keratin Nanoparticles And Their In Vivo Biodistribution For Targeted Delivery, Helan Xu, Zhen Shi, Narendra Reddy, Yiqi Yang

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Highly water-stable nanoparticles of around 70 nm and capable of distributing with high uptake in certain organs of mice were developed from feather keratin. Nanoparticles could provide novel veterinary diagnostics and therapeutics to boost efficiency in identification and treatment of livestock diseases to improve protein supply and ensure safety and quality of food. Nanoparticles could penetrate easily into cells and small capillaries, surpass detection of the immune system, and reach targeted organs because of their nanoscale sizes. Proteins with positive and negative charges and hydrophobic domains enable loading of various types of drugs and, hence, are advantageous over synthetic polymers …

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Development And Testing Of An In-Stream Phosphorus Cycling Model For The Soil And Water Assessment Tool, Michael J. White, Daniel E. Storm, Aaron R. Mittelstet, Philip R. Busteed, Brian E. Haggard, Colleen Rossi Jan 2014

Development And Testing Of An In-Stream Phosphorus Cycling Model For The Soil And Water Assessment Tool, Michael J. White, Daniel E. Storm, Aaron R. Mittelstet, Philip R. Busteed, Brian E. Haggard, Colleen Rossi

Department of Agricultural and Biological Systems Engineering: Faculty Publications

The Soil and Water Assessment Tool is widely used to predict the fate and transport of phosphorus (P) from the landscape through streams and rivers. The current in-stream P submodel may not be suitable for many stream systems, particularly those dominated by attached algae and those affected by point sources. In this research, we developed an alternative submodel based on the equilibrium P concentration concept coupled with a particulate scour and deposition model. This submodel was integrated with the SWAT model and applied to the Illinois River Watershed in Oklahoma, a basin influenced by waste water treatment plant discharges and …

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The Hydraulic Conductivity Structure Of Gravel-Dominated Vadose Zones Within Alluvial Floodplains, Ronald B. Miller, Derek M. Heeren, Garey A. Fox, Todd Halihan, Daniel E. Storm, Aaron R. Mittelstet Jan 2014

The Hydraulic Conductivity Structure Of Gravel-Dominated Vadose Zones Within Alluvial Floodplains, Ronald B. Miller, Derek M. Heeren, Garey A. Fox, Todd Halihan, Daniel E. Storm, Aaron R. Mittelstet

Department of Agricultural and Biological Systems Engineering: Faculty Publications

The floodplains of many gravel-bed streams have a general stratigraphy that consists of a layer of topsoil covering gravel-dominated subsoil. Previous research has demonstrated that this stratigraphy can facilitate preferential groundwater flow through focused linear features, such as paleochannels, or gravelly regions within the vadose zone. These areas within the floodplain vadose zone may provide a route for interactions between the floodplain surface and alluvial groundwater, effectively extending the hyporheic zone across the floodplain during high stream stage. The objective of this research was to assess the structure and scale of texture heterogeneity within the vadose zone within the gravel …

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Simulation Supported Estimation Of End-To-End Transmission Parameters In Non-Viral Gene Delivery, Beata J. Wysocki, Tadeusz A. Wysocki, Timothy M. Martin, Angela K. Pannier Jan 2014

Simulation Supported Estimation Of End-To-End Transmission Parameters In Non-Viral Gene Delivery, Beata J. Wysocki, Tadeusz A. Wysocki, Timothy M. Martin, Angela K. Pannier

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Communications, in general, involve delivery of information from a source to a sink. At nano-scale, an example of a man-made communications involving interfacing with biological systems at intra-cellular level is non-viral gene delivery. From a telecommunications engineering perspective, important end-to-end parameters of such a system are: the endto- end delay, system capacity, and packet loss rate. There are neither known methods to estimate those parameters theoretically nor they are ready available from standard measurements. The paper provides estimates for those parameters based on the simulation of non-viral gene delivery system based on the queuing theory. The simulator used has been …

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Alginate/Silica Hybrid Materials For Immobilization Of Green Microalgae Chlorella Vulgaris For Cellbased Sensor Arrays, Angela K. Pannier, Ulrich Soltmann, Bettina Soltmann, Rolf Altenburger, Mechthild Schmitt-Jansen Jan 2014

Alginate/Silica Hybrid Materials For Immobilization Of Green Microalgae Chlorella Vulgaris For Cellbased Sensor Arrays, Angela K. Pannier, Ulrich Soltmann, Bettina Soltmann, Rolf Altenburger, Mechthild Schmitt-Jansen

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Thin layers and patterned dot arrays of sodium alginate containing living microalgal cells were deposited onto glass carriers which were subsequently gelled using amino-functionalized silica sol to obtain reinforced alginate hydrogels. The resulting alginate/silica hybrid materials showed improved stability in salt-containing solutions compared to alginate gels gelled by traditional methods using Ca2+-ions. Cell arrays were patterned by printing nanolitre-scale drops of sodium alginate/cell suspension using a noncontact micro-dosage system which allows the printing of solutions of high viscosity. Cultures of the green microalga Chlorella vulgaris were immobilized within the newly developed alginate/silica hydrogels in order to demonstrate the …

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In Vitro Development Of Preimplantation Porcine Embryos Using Alginate Hydrogels As A Three-Dimensional Extracellular Matrix, Catherine Sargus-Patino, Elane C. Wright, Sarah A. Plautz, Jeremy R. Miles, Jeff L. Vallet, Angela K. Pannier Jan 2014

In Vitro Development Of Preimplantation Porcine Embryos Using Alginate Hydrogels As A Three-Dimensional Extracellular Matrix, Catherine Sargus-Patino, Elane C. Wright, Sarah A. Plautz, Jeremy R. Miles, Jeff L. Vallet, Angela K. Pannier

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Between Days 10 and 12 of gestation, porcine embryos undergo a dramatic morphological change, known as elongation, with a corresponding increase in oestrogen production that triggers maternal recognition of pregnancy. Elongation deficiencies contribute to embryonic loss, but exact mechanisms of elongation are poorly understood due to the lack of an effective in vitro culture system. Our objective was to use alginate hydrogels as three-dimensional scaffolds that can mechanically support the in vitro development of preimplantation porcine embryos. White cross-bred gilts were bred at oestrus (Day 0) to Duroc boars and embryos were recovered on Days 9, 10 or 11 of …

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Long-Term Activity Of Biohybrid Coatings Of Atrazine-Degrading Bacteria Pseudomonas Sp. Adp, Angela K. Pannier, T. Lehrer, M. Vogel, U. Soltmann, H. Bottcher, S. Tarre, M. Green, J. Raff, K. Pollmann Jan 2014

Long-Term Activity Of Biohybrid Coatings Of Atrazine-Degrading Bacteria Pseudomonas Sp. Adp, Angela K. Pannier, T. Lehrer, M. Vogel, U. Soltmann, H. Bottcher, S. Tarre, M. Green, J. Raff, K. Pollmann

Department of Agricultural and Biological Systems Engineering: Faculty Publications

The atrazine-degrading bacterial strain Pseudomonas sp. ADP was immobilized by the sol–gel process within thin silica layers coated onto water-retaining carrier materials (expanded clay pellets and scoria). The performance of the obtained biohybrid material has been investigated concerning long-term activity under non-growth conditions. Experiments were run in phosphate buffer containing atrazine (20 mg l-1) as the sole source of carbon and nitrogen. Even after one year of consecutive batch tests, P. ADP immobilized onto expanded clay pellets showed a high atrazine degradation activity. In the course of long-term batch experiments, the average amount of removed atrazine was about …

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Nutrient Concentrations Of Runoff As Affected By The Diameter Of Unconsolidated Material From Feedlot Surfaces, John E. Gilley, Gregory Boone, David B. Marx Jan 2014

Nutrient Concentrations Of Runoff As Affected By The Diameter Of Unconsolidated Material From Feedlot Surfaces, John E. Gilley, Gregory Boone, David B. Marx

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Beef cattle feedlots contain unconsolidated material that accumulates on the feedlot surface during a feeding cycle. This study was conducted to measure the effects of varying diameters of unconsolidated surface material and varying flow rates on nutrient concentrations in runoff. Unconsolidated surface material with an average diameter of 4.76, 9.53, 19.1, or 47.5 mm and a composite sample with a 15.2 mm mean diameter were placed within 0.75 m wide × 4.0 m long plot areas. Flow was then introduced at the top of the plots in successive increments, and runoff samples for water quality analyses were obtained. Particle diameter …

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Dynamic Analysis Of Dna Nanoparticle Immobilization To Model Biomaterial Substrates Using Combinatorial Spectroscopic Ellipsometry And Quartz Crystal Microbalance With Dissipation, Tadas Kasputis, Alex Pieper, Mathias Schubert, Angela K. Pannier Jan 2014

Dynamic Analysis Of Dna Nanoparticle Immobilization To Model Biomaterial Substrates Using Combinatorial Spectroscopic Ellipsometry And Quartz Crystal Microbalance With Dissipation, Tadas Kasputis, Alex Pieper, Mathias Schubert, Angela K. Pannier

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Gene expression within cells can be altered through gene delivery approaches, which have tremendous potential for gene therapy, tissue engineering, and diagnostics. Substrate-mediated gene delivery describes the delivery of plasmid DNA or DNA complexed with nonviral vectors to cells from a surface, with the DNA immobilized to a substrate through specific or nonspecific interactions. In this work, DNA-nanoparticle (DNA–NP) adsorption to substrates is evaluated using combinatorial, in situ spectroscopic ellipsometry and quartz crystal microbalance with dissipation (SE/QCM-D), to evaluate the basic dynamic processes involved in the adsorption and immobilization of DNA–NP complexes to substrates. The concentration of DNA–NP solutions influences …

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