Other Civil and Environmental Engineering Commons^™

Corn Fiber As A Biomass Feedstock For Production Of Succinic Acid, Lisbeth Vallecilla-Yepez, Divya Ramchandran, Dianna Long, Rajib Saha, Mark R. Wilkins

Department of Agricultural and Biological Systems Engineering: Faculty Publications

The selection of an economical carbon source is a fundamental parameter to establish a successful industrial succinic acid (SA) bioprocess. In this work, corn fiber (CF), a renewable and an inexpensive source of carbohydrates, was successfully used for bioproduction of SA. Optimized liquid hot water (LHW) pretreatment followed by enzymatic hydrolysis were used to obtain corn fiber hydrolysate (CFH). Results in batch fermentation with Actinobacillus succinogenes showed that a control solution mimicking CFH produced 28.7 g/L of SA with a yield of 0.67 g SA/g sugars, while fermentation of CFH produced 27.8 g/L of SA with a yield of 0.61 …

Modeling Watershed Sensitivity To Climate Change In Systems Affected By Discharge Of Mine Tailings, Johnette C. Shockley

Department of Civil and Environmental Engineering: Dissertations, Theses, and Student Research

For more than a century, a large volume of gold-mining tailings was deposited in Whitewood Creek and the Belle Fourche River, tributaries to the Cheyenne River in western South Dakota. Much of it still remains, and field and historical evidence indicates continued remobilization of tailings-containing alluvium in these bedrock-dominated channels. Both long-term, natural fluctuations in climate and anthropogenically driven changes can impact regional precipitation, temperature, hydrologic patterns, and ecosystem functions. Such changes have the potential to affect both the transport and distribution of arsenic-laden sediments and mechanical erosion that can undermine the stability of channel-bed and overbank material.

This study …

Greenhouse Gas Emissions And Drought Projections For Nebraska, Adam Liska

Adam Liska Papers

This lecture will focus on three issues. The first is a presentation of data from the first greenhouse gas emissions inventory for the State of Nebraska, which will be published in the coming weeks. In 2016, emissions from beef cattle made up 23% of net state emissions, and coal for electricity made up 23.7% of net state emissions. The second issue to be addressed are the impacts of the 2012 drought on agriculture in Nebraska and the region. The drought of 2012 indicates that future droughts in the 21^st century in the region can be a dominant influence on …

Inter-Relationships Between Water Depletion And Temperature Differential In Row Crop Canopies In A Sub-Humid Climate, Jasreman Singh, Yufeng Ge, Derek M. Heeren, Elizabeth Walter-Shea, Christopher M.U. Neale, Suat Irmak, Wayne Woldt, Geng Bai, Sandeep Bhatti, Mitchell S. Maguire

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Irrigation has a great impact on global food security as it contributes to the majority of the world’s agricultural food supply. It is essential to judiciously utilize water resources through efficient irrigation management since the majority of U.S. groundwater aquifers are rapidly depleting. Thus, quantification of the relationships between water depletion and environmental factors is important for understanding crop response to varying levels of water stresses that depletion can cause. The objectives of this research were to: 1) investigate the relationship between root zone water depletion (D_rw) and canopy temperature differential (ΔT) at different ranges of D_{rw …}

Characterization Of Tea (Camellia Sinensis) Granules For Quality Grading Using Computer Vision System, Md Towfiqur Rahman, Sabiha Ferdous, Mariya Sultana Jenin, Tanjina Rahman Mim, Masud Alam, Muhammad Rashed Al Mamun

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Tea (Camellia sinensis) has been found as an important medicinal beverage for human which is consumed all over the world. Primarily, the majority of tea is being cultivated in Asia and Africa, however it is commercially produced by more than 60 countries. Though substantial amount is produced, its processing system is still underdeveloped which leads to decrease in export opportunity as well as low monetary value. Moreover, the traditional method of tea grading and sorting is laborious, inefficient, and costly which ultimately produces the low-quality heterogeneous products. Processing and grading of tea granules after drying is very important …

Disturbance Triggers Non-Linear Microbe–Environment Feedbacks, Aditi Sengupta, Sarah J. Fansler, Rosalie K. Chu, Robert E. Danczak, Vanessa A. Garayburu-Caruso, Lupita Renteria, Hyun-Seob Song, Jason Toyoda, Jacqueline Wells, James C. Stegen

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Conceptual frameworks linking microbial community membership, properties, and processes with the environment and emergent function have been proposed but remain untested. Here we refine and test a recent conceptual framework using hyporheic zone sediments exposed to wetting–drying transitions. Our refined framework includes relationships between cumulative properties of a microbial community (e.g., microbial membership, community assembly properties, and biogeochemical rates), environmental features (e.g., organic matter thermodynamics), and emergent ecosystem function. Our primary aim was to evaluate the hypothesized relationships that comprise the conceptual framework and contrast outcomes from the whole and putatively active bacterial and archaeal communities. Throughout the system we …

Making Space For Social And Emotional Learning In Science Education, Erin Ingram, Kristie Reddick, Jessica M. Honaker, Gwen A. Pearson

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Social-emotional learning (SEL) is known to improve student outcomes but is rarely combined with STEM. In this paper we present an action research study to examine the impact of a STEM + SEL curriculum intervention to address a real-world school conflict. One hundred sixth–eighth graders and four teachers participated in an in-person facilitation of a SEL Arthropod curriculum, DIFFERENT. After the intervention, students completed open-ended couplet statements about arthropod behavior, tarantulas, and humans designed to measure sentiment change. Answers were manually coded using inductive coding on a scale of negative (1) to positive (5). Statement sentiments significantly shifted from negative …

Probabilistic Seismic Vulnerability Assessment Of Freestanding Historic Monuments, Marie E. Wagner

Department of Civil and Environmental Engineering: Dissertations, Theses, and Student Research

Seismic events can cause significant damage to structures, especially to freestanding structures which are not fixed to the ground and are free to rock independently. This thesis will study the response of freestanding statues and monuments to ground motions and develop probabilistic models for overturning, in order to assist in retrofitting and repair efforts. To do this, single and dual block system models were created with a variety of sizes, aspect ratios, and asymmetries, then subjected to several historic ground motions to determine whether they overturn using three-dimensional distinct element analysis as a result of rocking, sliding or twisting in …

Prioritization Of Research On Bridge Railings For Use On Timber Deck Bridges And Development Of Two Glulam Bridge Railing Systems, Jared Duren

Department of Civil and Environmental Engineering: Dissertations, Theses, and Student Research

In the past, many timber bridge railings, bridge railings for use on timber deck bridges, and approach guardrail transition systems to accompany these railings, have been developed to meet different impact safety criteria. Through an extensive literature review, the design details of these systems were identified and organized. A survey was then developed and distributed to multiple government agencies and timber industry members to identify their desire for these bridge railing and approach guardrail transition systems to be developed to meet current American Association of State Highway and Transportation Officials (AASHTO) Manual for Assessing Safety Hardware (MASH) impact safety criteria. …

Establishing Safe Operating Speeds For Autonomous Vehicles: A Case Study From The Automated Skyway Express In Jacksonville, Florida, Andrew Loken

Department of Civil and Environmental Engineering: Dissertations, Theses, and Student Research

Autonomous vehicles (AVs) differ significantly from traditional passenger vehicles in both their behavior and physical characteristics. As such, the validity of the guidance provided in the Manual for Assessing Safety Hardware, Second Edition (MASH 2016) is questionable in AV applications. Impact conditions specified in MASH 2016 are inextricably linked to the traditional vehicles underlying the estimates. For AV applications, conditions must be estimated from the ground up, stepping outside the guidance of MASH 2016. Herein, a method for evaluating existing infrastructure to support AV traffic is proposed. The method integrates traditional structural analyses with unconventional methods of estimating impact conditions. …

Oxygen Reactive Polymers For Treatment Of Traumatic Brain Injury, Parick S. Stayton, Menko P. Ypma, Peter A. Chiarelli, Joshua Sang Hun Park, Richard G. Ellenbogen, Julia Mengyun Xu, Pierre D. Mourad, Donghoon Lee, Anthony Convertime, Forrest M. Kievit

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Methods and compositions for treating traumatic brain injury . The methods and compositions utilize a multi - functional oxygen reactive polymer ( ORP ) that includes repeating units that include a reactive oxygen species ( ROS ) scavenging group and a polyalkylene oxide group . For theranostic applications , the oxygen reactive polymer fur ther includes a diagnostic group .

Determinants Of Water System Management, Caleb White

Department of Civil and Environmental Engineering: Dissertations, Theses, and Student Research

Water system management is complex, involving state, local, and federal regulations and impacting people from a wide variety of socioeconomic backgrounds. Systems in the developing and industrialized worlds both face challenges in managing water systems. Much research has been completed regarding associations of water system violations and system factors, and there has been an increased attention to issues of environmental justice – how water system violations affect communities of color, impoverished communities, and rural communities. Currently no studies have focused on the associations between socioeconomic factors and water system management violations. The goal of this research was to ascertain the …

Fragility Functions Of Manufactured Houses Under Earthquake Loads, Shuyah Tani Aurore Ouoba

Department of Civil and Environmental Engineering: Dissertations, Theses, and Student Research

Manufactured homes are factory-built homes made of wooden structural members, then transported and installed on a given site. Manufactured housing is used in many countries, such as in Australia, and in New Zealand but remain mostly popular in the United States. In 2020, nearly 22 million people were estimated to live in manufactured homes in the United States, with an increase of more than fifty percent in the shipment over the past seven years. However, performance observations from the last decades have shown the vulnerability of manufactured homes to extreme events, like windstorms and earthquakes. Damage assessments and post-event evaluations …

Robotic Technologies For High-Throughput Plant Phenotyping: Contemporary Reviews And Future Perspectives, Abbas Atefi, Yufeng Ge, Santosh Pitla, James Schnable

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Phenotyping plants is an essential component of any effort to develop new crop varieties. As plant breeders seek to increase crop productivity and produce more food for the future, the amount of phenotype information they require will also increase. Traditional plant phenotyping relying on manual measurement is laborious, time-consuming, error-prone, and costly. Plant phenotyping robots have emerged as a high-throughput technology to measure morphological, chemical and physiological properties of large number of plants. Several robotic systems have been developed to fulfill different phenotyping missions. In particular, robotic phenotyping has the potential to enable efficient monitoring of changes in plant traits …

Comparison Of Promoter, Dna Vector, And Cationic Carrier For Efficient Transfection Of Hmscs From Multiple Donors And Tissue Sources, Tyler Kozisek, Andrew Hamann, Luke Samuelson, Miguel Fudolig, Angela K. Pannier

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Human mesenchymal stem cells (hMSCs) are primary cells with high clinical relevance that could be enhanced through genetic modification. However, gene delivery, particularly through nonviral routes, is inefficient. To address the shortcomings of nonviral gene delivery to hMSCs, our lab has previously demonstrated that pharmacological “priming” of hMSCs with clinically approved drugs can increase transfection in hMSCs by modulating transfection-induced cytotoxicity. However, even with priming, hMSC transfection remains inefficient for clinical applications. This work takes a complementary approach to addressing the challenges of transfecting hMSCs by systematically investigating key transfection parameters for their effect on transgene expression. Specifically, we investigated …

Uavs For Vegetation Monitoring: Overview And Recent Scientific Contributions, Ana I. De Castro, Yeyin Shi, Joe Mari Maja, Jose M. Peña

Department of Agricultural and Biological Systems Engineering: Faculty Publications

This paper reviewed a set of twenty-one original and innovative papers included in a special issue on UAVs for vegetation monitoring, which proposed new methods and techniques applied to diverse agricultural and forestry scenarios. Three general categories were considered: (1) sensors and vegetation indices used, (2) technological goals pursued, and (3) agroforestry applications. Some investigations focused on issues related to UAV flight operations, spatial resolution requirements, and computation and data analytics, while others studied the ability of UAVs for characterizing relevant vegetation features (mainly canopy cover and crop height) or for detecting different plant/crop stressors, such as nutrient content/deficiencies, water …

Early Detection Of Encroaching Woody Juniperus Virginiana And Its Classification In Multi-Species Forest Using Uas Imagery And Semantic Segmentation Algorithms, Lin Wang, Yuzhen Zhou, Qiao Hu, Zhenghong Tang, Yufeng Ge, Adam Smith, Tala Awada, Yeyin Shi

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Woody plant encroachment into grasslands ecosystems causes significantly ecological destruction and economic losses. Effective and efficient management largely benefits from accurate and timely detection of encroaching species at an early development stage. Recent advances in unmanned aircraft systems (UAS) enabled easier access to ultra-high spatial resolution images at a centimeter level, together with the latest machine learning based image segmentation algorithms, making it possible to detect small-sized individuals of target species at early development stage and identify them when mixed with other species. However, few studies have investigated the optimal practical spatial resolution of early encroaching species detection. Hence, we …

Risk And Safety Associated With Hazardous Materials Transport In Rural And Native American Communities: A Case Study Of Thurston County, Nebraska, Sydney James

Department of Civil and Environmental Engineering: Dissertations, Theses, and Student Research

Rural counties and Native American Reservations often lack the resources and planning to respond to a hazardous material release which can cause catastrophic results. Many rural counties and Native American Reservations do not have close access to a fire department or medical facility, which would increase response times during a time of crisis. Jurisdiction issues further complicate the matter since Native American Reservations are sovereign nations,.

Thurston County, home to two Native American Reservations, served as a case study to perform a safety analysis for vehicles transporting hazardous material. Stakeholders within the county from tribal, county, and state levels were …

Evaluation Of Permanent Concrete Barriers To Mash 2016, Samuel Elias Hovde

Department of Civil and Environmental Engineering: Dissertations, Theses, and Student Research

The objective of this research project was to develop evaluation criteria for permanent concrete barriers to MASH 2016 TL-3 and TL-4 without crash testing and then to evaluate permanent concrete barrier standards provided by the sponsoring agencies of the Midwest Pooled Fund Program. A literature review was conducted to determine evaluation criteria for stability, shape, strength, and anchorage, and a database of permanent concrete barrier crash tests was compiled. A survey was conducted to collect barrier standards from the members of the Midwest Pooled Fund Program, and barriers were selected and evaluated according to the determined methodology. A total of …

Impact Of Particle Surface Charge Heterogeneity On Deposition Onto Flat Surfaces And Transport In Porous Media, Thompson Delon

Department of Civil and Environmental Engineering: Dissertations, Theses, and Student Research

Biological and non-biological natural colloids are anisotropic and ubiquitously exist in groundwater. Previously in colloid transport modelling, colloids are assumed to be homogenous for simplification, whether in shape or surface charge. More research has been done to incorporate surface heterogeneity in the transport experiment, i.e., surface charge heterogeneity on collector in column experiment. However, few studies have been done on surface heterogeneity on colloids themselves.

In this dissertation, Janus particles with different surface charge was developed to model surface heterogeneity on colloids. The interaction energy between Janus particle and flat surfaces was analyzed through DLVO simulation. It was discovered that …

Utilizing Digital Image Processing And Two‑Source Energy Balance Model For The Estimation Of Evapotranspiration Of Dry Edible Beans In Western Nebraska, Wei‑Zhen Liang, Isabella Possignolo, Xin Qiao, Kendall Dejonge, Suat Irkmak, Derek M. Heeren, Daran Rudnick

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Having an accurate yet simple method to estimate crop evapotranspiration ( ET_C) is a vital component of reliable irrigation scheduling. In this study, two versions of the two-source energy balance (TSEB) model: the TSEB model with the Priestley–Taylor equation (TSEB-PT) and the Penman–Monteith equation (TSEB-PM), were used to estimate ET_C of dry edible beans in western Nebraska. Compared with previous studies, this study is unique in that a Visual Basic software—Crop Canopy Image Analyzer (CCIA) was developed to process digitally captured RGB canopy images to obtain necessary canopy cover (CC) parameters for the TSEB models such as …

Comparative Study Of Human Pluripotent Stem Cell-Derived Endothelial Cells In Hydrogel-Based Culture Systems, Zhanqi Wang, Fuxing Zuo, Qing Liu, Xuesheng Wu, Qian Du, Yuguo Lei, Zhangmin Wu, Haishuang Lin

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Human pluripotent stem cell (hPSC)-derived endothelial cells (ECs) are promising cell sources for drug discovery, tissue engineering, and studying or treating vascular diseases. However, hPSC-ECs derived from different culture methods display different phenotypes. Herein, we made a detailed comparative study of hPSC-ECs from three different culture systems (e.g., 2D, 3D PNIPAAm-PEG hydrogel, and 3D alginate hydrogel cultures) based on our previous reports. We expanded hPSCs and differentiated them into ECs in three culture systems. Both 3D hydrogel systems could mimic an in vivo physiologically relevant microenvironment to protect cells from shear force and prevent cell agglomeration, leading to a high …

Determination Of Vadose Zone And Saturated Zone Nitrate Lag Times Using Long-Term Groundwater Monitoring Data And Statistical Machine Learning, Martin J. Wells, Troy E. Gilmore, Natalie Nelson, John K. Bohlke

Department of Agricultural and Biological Systems Engineering: Faculty Publications

In this study, we explored the use of statistical machine learning and long-term groundwater nitrate monitoring data to estimate vadose zone and saturated zone lag times in an irrigated alluvial agricultural setting. Unlike most previous statistical machine learning studies that sought to predict groundwater nitrate concentrations within aquifers, the focus of this study was to leverage available groundwater nitrate concentrations and other environmental variables to determine mean regional vertical velocities (transport rates) of water and solutes in the vadose zone and saturated zone (3.50 and 3.75 m yr−1 , respectively). The statistical machine learning results are consistent with two primary …

Role Of Nadþ In Regulating Cellular And Metabolic Signaling Pathways, Sara Amjad, Sabah Nisar, Ajaz A. Bhat, Ab Rauf Shah, Michael P. Frenneaux, Khalid Fakhro, Mohammad Haris, Ravinder Reddy, Zoltan Patay, Joseph Baur, Puneet Bagga

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Background: Nicotinamide adenine dinucleotide (NAD⁺), a critical coenzyme present in every living cell, is involved in a myriad of metabolic processes associated with cellular bioenergetics. For this reason, NAD⁺ is often studied in the context of aging, cancer, and neurodegenerative and metabolic disorders.

Scope of review: Cellular NAD⁺ depletion is associated with compromised adaptive cellular stress responses, impaired neuronal plasticity, impaired DNA repair, and cellular senescence. Increasing evidence has shown the efficacy of boosting NAD⁺ levels using NAD⁺ precursors in various diseases. This review provides a comprehensive understanding into the role of NAD^{+ …}

Historical Contingency In Microbial Resilience To Hydrologic Perturbations, Hyun-Seob Song, James C. Stegen, Emily B. Graham, Timothy D. Scheibe

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Development of reliable biogeochemical models requires a mechanistic consideration of microbial interactions with hydrology. Microbial response to and its recovery after hydrologic perturbations (i.e., resilience) is a critical component to understand in this regard, but generally difficult to predict because the impacts of future events can be dependent on the history of perturbations (i.e., historical contingency). Fundamental issues underlying this phenomenon include how microbial resilience to hydrologic perturbations is influenced by historical contingency and how their relationships vary depending on the characteristics of microbial functions. To answer these questions, we considered a simple microbial community composed of two species that …

Quantification Of Nitric Oxide Concentration Using Single-Walled Carbon Nanotube Sensors, Jakob Meier, Joseph Stapleton, Eric M. Hofferber, Abigail Haworth, Stephen D. Kachman, Nicole M. Iverson

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Nitric oxide (NO), a free radical present in biological systems, can have many detrimental effects on the body, from inflammation to cancer. Due to NO’s short half-life, detection and quan- tification is difficult. The inability to quantify NO has hindered researchers’ understanding of its impact in healthy and diseased conditions. Single-walled carbon nanotubes (SWNTs), when wrapped in a specific single-stranded DNA chain, becomes selective to NO, creating a fluorescence sensor. Unfortunately, the correlation between NO concentration and the SWNT’s fluorescence intensity has been difficult to determine due to an inability to immobilize the sensor without altering its properties. Through the …

Modeling And Prioritizing Interventions Using Pollution Hotspots For Reducing Nutrients, Atrazine And E. Coli Concentrations In A Watershed, Olufemi P. Abimbola, Aaron R. Mittelstet, Tiffany Messer, Elaine Berry, Anna Van Griensven

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Excess nutrients and herbicides remain two major causes of waterbody impairment globally. In an attempt to better understand pollutant sources in the Big Sandy Creek Watershed (BSCW) and the prospects for successful remediation, a program was initiated to assist agricultural producers with the implementation of best management practices (BMPs). The objectives were to (1) simulate BMPs within hotspots to determine reductions in pollutant loads and (2) to determine if water-quality standards are met at the watershed outlet. Regression-based load estimator (LOADEST) was used for determining sediment, nutrient and atrazine loads, while artificial neural networks (ANN) were used for determining E. …

Antibiotic Resistance Genes In Swine Manure Slurry As Affected By Pit Additives And Facility Disinfectants, Maria C. Hall, Jon Duerschner, John E. Gilley, Amy Schmidt, Shannon Bartelt-Hunt, Daniel D. Snow, Kent M. Eskridge, Xu Li

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Manure storage facilities are critical control points to reduce antibiotic resistance genes (ARGs) in swine manure slurry before the slurry is land applied. However, little is known about how exogenous chemicals entering the manure storage facilities may affect the fate of ARGs. The objective of this study was to analyze the impact of six commonly used pit additives and four facility disinfectants on the concentration of ARGs in swine manure slurry. Bench scale reactors, each containing approximately 50 L of liquid swine manure, were dosed with additives or disinfectants and were sampled for 40 days. Seven antibiotic resistance genes along …

Differential Survival Of Non-O157 Shiga Toxigenic Escherichia Coli In Simulated Cattle Feedlot Runoff, Lisa M. Durso, John E. Gilley, Daniel N. Miller

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Environmental survival time is important when evaluating adverse health outcomes from foodborne pathogens. Although outbreaks associated with manure-impacted irrigation or runoff water are relatively infrequent, their broad scope, regulatory importance, and severe health outcomes highlight the need to better understand the environmental survival of manure-borne pathogens. Shiga toxigenic Escherichia coli (STEC) are excreted in feces and persist in the environment until they die or recolonize a new host. Surface waters contaminated with manure-borne STEC can infect humans through drinking and recreational water use or irrigated crops that are minimally cooked. In this study, manure-impacted water microcosms mimicking beef cattle feedlot …

Editorial: Predictive Modeling Of Human Microbiota And Their Role In Health And Disease, Hyun-Seob Song, Stephen R. Lindemann, Dong-Yup Lee

Department of Agricultural and Biological Systems Engineering: Faculty Publications

No abstract provided.