Other Civil and Environmental Engineering Commons^™

Nucleic Acid Delivery To Mesenchymal Stem Cells: A Review Of Nonviral Methods And Applications, Andrew Hamann, Albert Nguyen, Angela K. Pannier

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Background: Mesenchymal stem cells (MSCs) are multipotent stem cells that can be isolated and expanded from many tissues, and are being investigated for use in cell therapies. Though MSC therapies have demonstrated some success, none have been FDA approved for clinical use. MSCs lose stemness ex vivo, decreasing therapeutic potential, and face additional barriers in vivo, decreasing therapeutic efficacy. Culture optimization and genetic modification of MSCs can overcome these barriers. Viral transduction is efficient, but limited by safety concerns related to mutagenicity of integrating viral vectors and potential immunogenicity of viral antigens. Nonviral delivery methods are safer, though limited …

Water Effects On Optical Canopy Sensing For Late-Season Site-Specific Nitrogen Management Of Maize, Tsz Him Lo, Daran Rudnick, Brian Krienke, D. M. Heeren, Yufeng Ge, Tim M. Shaver

Department of Agricultural and Biological Systems Engineering: Faculty Publications

The interpretation of optical canopy sensor readings for determining optimal rates of late-season site-specific nitrogen application to corn (Zea mays L.) can be complicated by spatially variable water sufficiency, which can also affect canopy size and/or pigmentation. In 2017 and 2018, corn following corn and corn following soybeans were subjected to irrigation×nitrogen fertilizer treatments in west central Nebraska, USA, to induce variable water sufficiency and variable nitrogen sufficiency. The vegetation index-sensor combinations investigated were the normalized difference vegetation index (NDVI), the normalized difference red edge index (NDRE), and the reflectance ratio of near infrared minus red edge over near …

Principal Variable Selection To Explain Grain Yield Variation In Winter Wheat From Features Extracted From Uav Imagery, Jiating Li, Arun-Narenthiran Veeranampalayam-Sivakumar, Madhav Bhatta, Nicholas D. Garst, Hannah Stoll, P. Stephen Baenziger, Vikas Belamkar, Reka Howard, Yufeng Ge, Jiating Li

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Background: Automated phenotyping technologies are continually advancing the breeding process. However, collecting various secondary traits throughout the growing season and processing massive amounts of data still take great efforts and time. Selecting a minimum number of secondary traits that have the maximum predictive power has the potential to reduce phenotyping efforts. The objective of this study was to select principal features extracted from UAV imagery and critical growth stages that contributed the most in explaining winter wheat grain yield. Five dates of multispectral images and seven dates of RGB images were collected by a UAV system during the spring growing …

Agenator: An Open Source Computer-Controlled Dry Aging System For Beef, Soon Kiat Lau, Felipe Azevedo Ribeiro, Jeyamkondan Subbiah, Chris R. Calkins

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Dry aging of beef is a process where beef is exposed to a controlled environment with the ultimate goal of drying the beef to improve its quality and value. Comprehensive investigations into the effects of various environmental conditions on dry aging are crucial for understanding and optimizing the process, but the lack of affordable equipment focused on data collection makes it difficult to do so. The Agenator was thus developed as an open source system with a suite of features for investigating dry aging such as: measuring and recording relative humidity, temperature, mass, air velocity, and fan rotational speed; precise …

Early Prediction Of Soybean Traits Through Color And Texture Features Of Canopy Rgb Imagery, Wenan Yuan, Nuwan Kumara Wijewardane, Shawn Jenkins, Geng Bai, Yufeng Ge, George L. Graef

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Global crop production is facing the challenge of a high projected demand, while the yields of major crops are not increasing at sufficient speeds. Crop breeding is an important way to boost crop productivity, however its improvement rate is partially hindered by the long crop generation cycles. If end-season crop traits such as yield can be predicted through early-season phenotypic measurements, crop selection can potentially be made before a full crop generation cycle finishes. This study explored the possibility of predicting soybean end-season traits through the color and texture features of early-season canopy images. Six thousand three hundred and eighty-three …

Heterologous Expression And Functional Characterization Of A Gh10 Endoxylanase From Aspergillus Fumigatus Var. Niveus With Potential Biotechnological Application, Josman Velasco, Bianca Oliva, Evandro Jose Mulinari, Leidy Patricia Quintero, Awana Da Silva Lima, Aline Larissa Goncalves, Thiago Augusto Goncalves, Andre Damasio, Fabio Marcio Squina, Adriane Maria Ferreira Milagres, Asmaa Abdella, Mark R. Wilkins, Fernando Segato

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Xylanases decrease the xylan content in pretreated biomass releasing it from hemicellulose, thus improving the accessibility of cellulose for cellulases. In this work, an endo-β-1,4-xylanase from Aspergillus fumigatus var. niveus (AFUMN-GH10) was successfully expressed. The structural analysis and biochemical characterization showed this AFUMN-GH10 does not contain a carbohydrate-binding module. The enzyme retained its activity in a pH range from 4.5 to 7.0, with an optimal temperature at 60°C. AFUMN-GH10 showed the highest activity in beechwood xylan. The mode of action of AFUMNGH10 was investigated by hydrolysis of APTS-labeled xylohexaose, which resulted in xylotriose and xylobiose as the main products. AFUMN-GH10 …

Antibiotic Resistance Gene Profile Changes In Cropland Soil After Manure Application And Rainfall, Morgan Meyers, Lisa Durso, John E. Gilley, Heidi Waldrip, Lana Castleberry, Amy Millmier Schmidt

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Land application of manure introduces gastrointestinal microbes into the environment, including bacteria carrying antibiotic resistance genes (ARGs). Measuring soil ARGs is important for active stewardship efforts to minimize gene flow from agricultural production systems; however, the variety of sampling protocols and target genes makes it difficult to compare ARG results between studies. We used polymerase chain reaction (PCR) methods to characterize and/or quantify 27 ARG targets in soils from 20 replicate, long-term no-till plots, before and after swine manure application and simulated rainfall and runoff. All samples were negative for the 10 b-lactamase genes assayed. For tetracycline resistance, only source …

Optimization Of Process Parameters And Fermentation Strategy For Xylanase Production In A Stirred Tank Reactor Using A Mutant Aspergillus Nidulans Strain, Asmaa Abdella, Fernando Segato, Mark R. Wilkins

Department of Agricultural and Biological Systems Engineering: Faculty Publications

The present work studied the optimization of aeration rate, agitation rate and oxygen transfer and the use of various batch fermentation strategies for xylanase production from a recombinant Aspergillus nidulans strain in a 3 L stirred tank reactor. Maximum xylanase production of 1250 U/mL with productivity of 313 U/mL/day was obtained under an aeration rate of 2 vvm and an agitation rate of 400 rpm using batch fermentation. The optimum volumetric oxygen transfer coefficient (kLa) for efficient xylanase production was found to be 38.6 h-1. Fed batch mode and repeated batch fermentation was also performed with kLa was 38.6 h …

System For Optimizing Fed-Batch Hydrolysis Of Biomass, Chao Tai, Deepak R. Keshwani

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Provided herein is a system for optimizing the fed-batch hydrolysis of biomass.

Modeled And Measured Ecosystem Respiration In Maize–Soybean Systems Over 10 Years, Ming Zhan, Adam Liska, Anthony Nguy-Robertson, Andrew E. Suyker, Matthew P. Pelton, Haishun Yang

Adam Liska Papers

Crop residue is an abundant resource for the potential production of biofuels, but a better understanding of its use on net carbon emissions must be developed to mitigate climate change. This analysis combines two established crop growth models (Hybrid-Maize and SoySim) with a simple soil and crop residue respiration model to estimate daily ecosystem respiration (ERe) from maize and soybean; ERe was estimated to be the sum of CO₂ emissions from the oxidation of the growing crop, crop residue, and soil organic carbon (SOC). Model-estimated CO₂ fluxes from irrigated continuous maize and irrigated maize–soybean cropping systems in eastern …

Water, Energy, And Carbon Footprints Of Bioethanol From The U.S. And Brazil, Mesfin Mekonnen, Thiago L. Romanelli, Chittaranjan Ray, Arjen Y. Hoekstra, Adam Liska, Christopher M. U. Neale

Adam Liska Papers

Driven by biofuel policies, which aim to reduce greenhouse gas (GHG) emissions and increase domestic energy supply, global production and consumption of bioethanol have doubled between 2007 and 2016, with rapid growth in corn-based bioethanol in the U.S. and sugar cane-based bioethanol in Brazil. Advances in crop yields, energy use efficiency in fertilizer production, biomass-to-ethanol conversion rates, and energy efficiency in ethanol production have improved the energy balance and GHG emission reduction potential of bioethanol. In the current study, the water, energy, and carbon footprints of bioethanol from corn in the U.S. and sugar cane in Brazil were assessed. The …

Invasion Of The P Elements: Tolerance Is Not Futile, Colin D. Meiklejohn, Justin P. Blumenstiel

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Organisms are locked in an eternal struggle with parasitic DNA sequences that live inside their genomes and wreak havoc on their host’s chromosomes as they spread through populations. To combat these parasites, host species have evolved elaborate mechanisms of resistance that suppress their activity. A new study in Drosophila indicates that, prior to the acquisition of resistance, individuals can vary in their ability to tolerate the activity of these genomic parasites, ignoring or repairing the damage they induce. This tolerance results from variation at genes involved in germline development and DNA damage checkpoints and suggests that these highly conserved cellular …

Cover Crops Have Negligible Impact On Soil Water In Nebraska Maize–Soybean Rotation, J. Burdette Barker, Derek M. Heeren, Katja Koehler-Cole, Charles Shapiro, Humberto Blanco-Canqui, Roger W. Elmore, Christopher A. Proctor, Suat Irmak, Charles A. Francis, Tim M. Shaver, Ali T. Mohammed

Department of Agricultural and Biological Systems Engineering: Faculty Publications

One perceived cost of integrating winter cover cropping in maize (Zea mays L.) and soybean [Glycine max (L.) Merr.] rotation systems is the potential negative impact on soil water storage available for primary crop production. The objective of this 3-year study was to evaluate the effects of winter cover crops on soil water storage and cover crop biomass production following no-till maize and soybean rotations. Locations were near Brule (west-central), Clay Center (south-central), Concord (northeast), and Mead (east-central), Nebraska, United States. Treatments included crop residue only (no cover crop) and a multi-species cover crop mix, both broadcast-seeded before …

Portable Flux Tower Deployments Field Campaign Report, M. S. Torn, L Kuepper, S. C. Biraud, D. P. Billesbach

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Contents

Acronyms and Abbreviations...................................................................... iii

1.0 Summary ....................................................... 1

2.0 Results ........................................... 1

3.0 Publications and References ................................................. 2

4.0 Lessons Learned .................................................................... 2

U.S. Agro-Climate In 20th Century: Growing Degree Days, First And Last Frost, Growing Season Length, And Impacts On Crop Yields, Meetpal Singh Kukal, Suat Irmak

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Significant air temperature changes have occurred globally during the 20th century, which are spatially variable to a considerable degree and these changes can have substantial implications in agroecosystem productivity. The agroclimate indicators that are responsible in these contexts are first fall frost (FFF), last spring frost (LSF), climatological growing season (CGS) length, and heat accumulation (growing degree days, GDD). We explore spatial and temporal trends associated with these indices across the continental U.S. (CONUS) during 1900–2014 using datasets collected at 1218 sites. On average, FFF has been occurring later (by 5.4 days century⁻¹), and LSF has been occurring …

Adam J. Liska: Curriculum Vitae, Adam Liska

Adam Liska Papers

Associate Professor, George Dempster Smith Chair of Industrial Ecology, Departments of Biological Systems Engineering and Agronomy & Horticulture, University of Nebraska-Lincoln 236 L.W. Chase Hall, Lincoln, NE 68583-0726, Ph: (402) 472-8744, e-mail: [email protected]

Ph.D. 2003 Biology (magna cum laude), Max Planck Institute of Molecular Cell Biology & Genetics, Technische Universität Dresden, Dresden, Germany

Climate-Driven Crop Yield And Yield Variability And Climate Change Impacts On The U.S. Great Plains Agricultural Production, Meetpal Singh Kukal, Suat Irmak

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Climate variability and trends affect global crop yields and are characterized as highly dependent on location, crop type, and irrigation. U.S. Great Plains, due to its significance in national food production, evident climate variability, and extensive irrigation is an ideal region of investigation for climate impacts on food production. This paper evaluates climate impacts on maize, sorghum, and soybean yields and effect of irrigation for individual counties in this region by employing extensive crop yield and climate datasets from 1968–2013. Variability in crop yields was a quarter of the regional average yields, with a quarter of this variability explained by …

Evaluation Of Variable Rate Irrigation Using A Remote-Sensing-Based Model, John Burdette Barker, Derek M. Heeren, Christopher M.U. Neale, Daran Rudnick

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Improvements in soil water balance modeling can be beneficial for optimizing irrigation management to account for spatial variability in soil properties and evapotranspiration (ET). A remote-sensing-based ET and water balance model was tested for irrigation management in an experiment at two University of Nebraska-Lincoln research sites located near Mead and Brule, Nebraska. Both fields included a center pivot equipped with variable rate irrigation (VRI). The study included maize in 2015 and 2016 and soybean in 2016 at Mead, and maize in 2016 at Brule, for a total of 210 plot-years. Four irrigation treatments were applied at Mead, including: VRI based …

Dynamic Rops Test For Tractors Over 6,000 Kilograms, Caleb M. Lindhorst, Roger M. Hoy, Santosh Pitla, Michael F. Kocher

Department of Agricultural and Biological Systems Engineering: Faculty Publications

OECD static tests (Codes 4, 6, 7, and 8) for agricultural rollover protective structures (ROPS) have become accepted standards for evaluating the ability of these structures to protect the operator during tractor rollover events. The strength properties of some materials typically used in ROPS change because of cold weather embrittlement at low temperatures. The static ROPS tests lack the ability to evaluate the strength of these structures during cold weather. The use of the dynamic ROPS test is well noted as a means for proving cold weather embrittlement resistance properties. Unfortunately, application of the OECD dynamic ROPS test (Code 3) …

The Open Source Gaitor Suite For Rodent Gait Analysis, Brittany Y. Jacobs, Emily H. Lakes, Alex J. Reiter, Spencer P. Lake, Trevor R. Ham, Nic D. Leipzig, Stacy L. Porvasnik, Christine E. Schmidt, Rebecca A. Wachs, Kyle D. Allen

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Locomotive changes are often associated with disease or injury, and these changes can be quantified through gait analysis. Gait analysis has been applied to preclinical studies, providing quantitative behavioural assessment with a reasonable clinical analogue. However, available gait analysis technology for small animals is somewhat limited. Furthermore, technological and analytical challenges can limit the effectiveness of preclinical gait analysis. The Gait Analysis Instrumentation and Technology Optimized for Rodents (GAITOR) Suite is designed to increase the accessibility of preclinical gait analysis to researchers, facilitating hardware and software customization for broad applications. Here, the GAITOR Suite’s utility is demonstrated in 4 models: …

Greenhouse Gas Emissions From Beef Feedlot Surface Materials As Affected By Diet, Moisture, Temperature, And Time, Bryan L. Woodbury, John E. Gilley, David B. Parker, Bobbi S. Stomer

Department of Agricultural and Biological Systems Engineering: Faculty Publications

A laboratory study was conducted to measure the effects of diet, moisture, temperature, and time on greenhouse gas (GHG) emissions from feedlot surface materials (FSM). The FSM were collected from open-lot pens where beef cattle were fed either a dry-rolled corn (DRC) diet containing no wet distillers grains with solubles (WDGS) or a DRC diet containing 35% WDGS. The FSM were collected, air-dried or mixed with 3.0 L of water to represent dry or wet conditions, and then incubated at temperatures of 5°C, 15°C, 25°C, or 35°C. Static flux chambers were used to quantify GHG emissions over a 14-day period. …

Surface Detention On Cropland, Rangeland, And Conservation Reserve Program Areas, John E. Gilley

Department of Agricultural and Biological Systems Engineering: Faculty Publications

One of the factors contributing to overland flow on upland areas is water stored temporarily in a thin sheet on the soil surface as surface detention. This study was conducted to quantify surface detention on selected cropland, rangeland, and Conservation Reserve Program (CRP) sites. Surface detention was determined from the recession portion of runoff hydrographs corresponding with the period when rainfall had ceased but runoff continued. The hydrographs were generated from six previously reported rainfall simulation studies conducted on paired 3.7 m wide  10.7 m long plots on which approximately 128 mm of rainfall was applied. Surface detention values …

Evaluation Of The Accuracy Of Machine Reported Can Data For Engine Torque And Speed (J1939), Rodney A. Rohrer, Joe D. Luck, Santosh K. Pitla, Roger M. Hoy

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Most modern off-road machinery use embedded electronic controllers connected to a controller area network (CAN) to broadcast machine information for on-board processes and diagnostics. Commercially available tools can record CAN data for a variety of research and commercial uses. For agricultural tractors, there is an opportunity to create advanced test procedures that are more representative of field operations and that could supplement existing machine performance tests, such as the OECD Code 2 Standard Code for the Official Testing of Agricultural and Forestry Tractor Performance. CAN parameters provide an efficient way to collect tractor performance data during field operations. However, the …

Crop Evapotranspiration, Irrigationwater Requirement And Water Productivity Of Maize From Meteorological Data Under Semiarid Climate, Koffi Djaman, Michael O'Neill, Curtis K. Owen, Daniel Smeal, Komlan Koudahe, Margaret West, Samuel Allen, Kevin Lombard, Suat Irmak

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Under the semiarid climate of the Southwest United States, accurate estimation of crop water use is important for water management and planning under conservation agriculture. The objectives of this study were to estimate maize water use and water productivity in the Four Corners region of New Mexico. Maize was grown under full irrigation during the 2011, 2012, 2013, 2014 and 2017 seasons at the Agricultural Science Center at Farmington (NM). Seasonal amounts of applied irrigation varied from 576.6 to 1051.6 mm and averaged 837.7 mm and the total water supply varied from 693.4 to 1140.5 mm. Maize actual evapotranspiration was …

Unmanned Aerial Systems-Based Remote Sensing For Monitoring Sorghum Growth And Development, Sanaz Shafian, Nithya Rajan, Ronnie Schnell, Muthukumar Bagavathiannan, John Valasek, Yeyin Shi, Jeff Olsenholler

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Unmanned Aerial Vehicles and Systems (UAV or UAS) have become increasingly popular in recent years for agricultural research applications. UAS are capable of acquiring images with high spatial and temporal resolutions that are ideal for applications in agriculture. The objective of this study was to evaluate the performance of a UAS-based remote sensing system for quantification of crop growth parameters of sorghum (Sorghum bicolor L.) including leaf area index (LAI), fractional vegetation cover (f_c) and yield. The study was conducted at the Texas A&M Research Farm near College Station, Texas, United States. A fixed-wing UAS equipped with a …

Rapeseed Seedling Stand Counting And Seeding Performance Evaluation At Two Early Growth Stages Based On Unmanned Aerial Vehicle Imagery, Biquan Zhao, Jian Zhang, Chenghai Yang, Guangsheng Zhou, Youchun Ding, Yeyin Shi, Dongyan Zhang, Jing Xie, Qingxi Liao

Department of Agricultural and Biological Systems Engineering: Faculty Publications

The development of unmanned aerial vehicles (UAVs) and image processing algorithms for field-based phenotyping offers a non-invasive and effective technology to obtain plant growth traits such as canopy cover and plant height in fields. Crop seedling stand count in early growth stages is important not only for determining plant emergence, but also for planning other related agronomic practices. The main objective of this research was to develop practical and rapid remote sensing methods for early growth stage stand counting to evaluate mechanically seeded rapeseed (Brassica napus L.) seedlings. Rapeseed was seeded in a field by three different seeding devices. A …

Nitric Oxide Sensors For Biological Applications, Nicole M. Iverson, Eric M. Hofferber, Joseph A. Stapleton

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Nitric oxide (NO) is an essential signaling molecule within biological systems and is believed to be involved in numerous diseases. As a result of NO’s high reaction rate, the detection of the concentration of NO, let alone the presence or absence of the molecule, is extremely difficult. Researchers have developed multiple assays and probes in an attempt to quantify NO within biological solutions, each of which has advantages and disadvantages. This review highlights many of the current NO sensors, from those that are commercially available to the newest sensors being optimized in research labs, to assist in the understanding and …

Evaluation Of Critical Nitrogen And Phosphorus Models For Maize Under Full And Limited Irrigation Conditions, Koffi Djaman, Suat Irmak

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Proper nitrogen (N) fertiliser application rates and timing of application, coupled with optimum irrigation management can improve the sustainability of maize production and reduce the risk of environmental contamination by nutrients. The impact of full and limited irrigation and rainfed conditions on in-season maize (Zea mays L.) shoot biomass nutrient concentration and critical N and phosphorus (P) indices were evaluated using a combination of measured nutrients and critical N and P models in south central Nebraska in 2009 and 2010. Four irrigation treatments [fully-irrigated treatment (FIT), 75% FIT, 60% FIT and 50% FIT) and rainfed] were imposed. Irrigation regimes …

Phenotypic Responses To Interspecies Competition And Commensalism In A Naturally Derived Microbial Co-Culture, Nymul Khan, Yukari Maezato, Ryan S. Mcclure, Colin J. Brislawn, Jennifer M. Mobberley, Nancy Isern, William B. Chrisler, Lye Meng Markillie, Brett M. Barney, Hyun-Seob Song, William C. Nelson, Hans C. Bernstein

Department of Agricultural and Biological Systems Engineering: Faculty Publications

The fundamental question of whether different microbial species will co-exist or compete in a given environment depends on context, composition and environmental constraints. Model microbial systems can yield some general principles related to this question. In this study we employed a naturally occurring co-culture composed of heterotrophic bacteria, Halomonas sp. HL-48 and Marinobacter sp. HL- 58, to ask two fundamental scientific questions: 1) how do the phenotypes of two naturally co-existing species respond to partnership as compared to axenic growth? and 2) how do growth and molecular phenotypes of these species change with respect to competitive and commensal interactions? We …

Impacts Of Cover Crops On Soil Physical Properties: Field Capacity, Permanent Wilting Point, Soil-Water Holding Capacity, Bulk Density, Hydraulic Conductivity, And Infiltration, Suat Irmak, Vivek Sharma, Ali T. Mohammed, Koffi Djaman

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Field experiments were carried out to quantify the effects of cover cropping on soil physical properties. Field capacity (FC), permanent wilting point (PWP), soil-water holding capacity (SWHC), bulk density (  b), saturated and unsaturated hydraulic conductivity (Ks and Kus, respectively), and infiltration rates were measured and compared for four land cover treatments [cover crop without seed maize (CC), seed maize followed by cover crop (SCCC), bare soil, and seed maize without cover crop (SC)] in three large-scale production fields (~64 ha each) with silt loam soil in the 2012-2013, 2013-2014, 2014-2015, and 2015-2016 cover crop growing seasons. All production …