Environmental Engineering Commons^™

Adaptive Laboratory Evolution Of Scenedesmus Obliquus For Increased Carbohydrate Content And Biomass Productivity, Nahel Ali

Master's Theses

The economics of microalgal bioproduct commercialization would benefit from increased accumulation of energy storage compounds, such as carbohydrates and lipids, and increased biomass productivity. This thesis explores two adaptive laboratory evolution strategies for improving Scenedesmus obliquus: single UV-mutagenesis and low light cultivation at a high dilution rate to produce cultigens with greater carbohydrate content and iterative UV-mutagenesis and selection under outdoor conditions in a raceway pond at a high dilution rate to increase biomass productivity.

Two cultigens were generated with the goal of increased carbohydrate content: K5 and K7. Both were mutagenized for 5 seconds and cultivated in 50-mL …

Dairy Manure Flushwater Treatment By Algae Raceway Ponds And Aerated Biofilm Reactors, Darin Son

Master's Theses

Nitrogen removal technologies can help dairy industries meet their nutrient management needs. This thesis investigated two separate treatment technologies for total ammonia nitrogen (TAN) removal: algae raceway ponds and aerated biofilm reactors. Six 1000- liter algae raceway ponds and four 1000-liter tote tanks, each equipped with 10 sheets of nonwoven geotextile (i.e., thermally bonded or needle-punched) biofilm substrate, were used to treat the effluent from a flush dairy in central coastal California (TAN = 251 mg/L, cBOD5 = 204 mg/L). For the algae raceway ponds (TAN loading rate = 7 - 35 g/m³-day among 7-, 10- and 14-day hydraulic residence …

Development Of Zeolitic Imidazolate Frameworks For Enhancing Post-Combustion Co2 Capture, Dustin Lee

Master's Theses

Post-combustion CO₂ capture is a promising approach for complementing other strategies to mitigate climate change. Liquid absorption is currently used to capture CO₂ from post-combustion flue gases. However, the high energy cost required to regenerate the liquid absorbents is a major drawback for this process. As a result, solid sorbents have been investigated extensively in recent years as alternative media to capture CO₂ from flue gases. For example, metal organic frameworks (MOFs) are nanoporous materials that have high surface areas, large pore volumes, and flexible designs. A large number of MOFs, however, suffer from 1) low CO …

Modeling Artificial Groundwater Recharge In The Santa Rosa Creek Watershed, Alexander J. Murray

Master's Theses

The Santa Rosa Creek Watershed is an approximately 48 mi2 large watershed located on the central coast of California. This watershed drains to the Pacific Ocean through Santa Rosa Creek as it passes through agricultural land and the town of Cambria. Historically the groundwater within the Santa Rosa Creek Watershed has been used for irrigation, municipal and domestic uses, and the creek is critical habitat steelhead trout. During dry years, there is less water for all uses. When low groundwater levels occur, water can be drawn out of the creek and into the soil, drying out steelhead habitat. Seven agricultural …

Surface Modifications Of Reverse Osmosis Membranes For Removal Of Bromide And Reduction Of Fouling, Joseph Seo

Master's Theses

Reverse osmosis (RO) is widely used for water reuse and desalination. Although RO membranes are known for their high salt rejection and practical permeate flux, their performance can be impaired by fouling, and their removal of some disinfection byproducts and their precursors (e.g., bromide, N-Nitrosodimethylamine [NDMA]) does not meet drinking water standards. RO membrane modifications have been widely studied to overcome these limitations. In this research, RO membranes were grafted with cationic polymers to induce a positive charge on the RO membrane surface. This modification aimed at enhancing the rejection of negatively charged bromide ions by removing them from solution …

Quantitative And Qualitative Error Re-Analysis Of Tidal Datasets, Ernesto Andrew Ledezma

Master's Theses

Tidal data forms the basis for understanding and quantifying sea-level rise and tidal statistics. While quality assurance methods based upon spectral and harmonic analysis have been applied to individual tidal records, these methods have not been used to assess global tidal datasets. In this thesis, four west coast tidal records were examined using harmonic analysis methods to investigate uncertainty on a monthly averaged basis. Uncertainty was identified using a method that quantifies time lag as a linear regression of height difference between a predicted and measured tidal height and the predicted rate of change of tidal height. Errors identified through …

Optimization And Longevity Of Functionalized Multi-Walled Carbon Nanotube-Enabled Membranes For Water Treatment, Madeleine Michael Isabella White

Master's Theses

Water scarcity is a growing concern at the global scale. Large scale water reuse is growing both in necessity and popularity. Before water reuse can be performed efficiently on a large scale or be used for potable supply, even indirectly, contaminants of emerging concern (CECs) will need to be treated at the full scale. Advanced oxidation processes (AOPs) are a form of advanced water treatment capable of treating a wide range of CECs. This study contributes to the growing field of AOPs and more specifically AOPs using ozone combined with functionalized multi-walled carbon nanotubes (MWCNTs). Ozonation of MWCNTs has been …

Analysis And Optimization Of Colorimetric Nanosensors For Rapid Detection Of Microbes In Water, Ruby A. Lang

Master's Theses

Access to safe water is a basic human right recognized by the United Nations General Assembly in 2010 (WHO, 2020). However, a least 2.2 billion people globally still are without safely managed water services meaning they use a drinking water source that can be contaminated with faeces (WHO, 2020). With such a pressing global health issue, it is clear that improvement to water systems is important and required in the Agenda 2030 Sustainable Development Goals (SDGs). However, to improve water systems and prove they are safe water sources, water quality testing must occur. A solution to this issue is the …

Investigation Of New Forward Osmosis Draw Agents And Prioritization Of Recent Developments Of Draw Agents Using Multi-Criteria Decision Analysis, Jodie Wei Yu

Master's Theses

Forward osmosis (FO) is an emerging technology for water treatment due to their ability to draw freshwater using an osmotic pressure gradient across a semi-permeable membrane. However, the lack of draw agents that could both produce reasonable flux and be separated from the draw solution at a low cost stand in the way of widespread implementation. This study had two objectives: evaluate the performance of three materials — peptone, carboxymethyl cellulose (CMC), and magnetite nanoparticles (Fe₃O₄ NPs) — as potential draw agents, and to use multi-criteria decision matrices to systematically prioritize known draw agents from literature for …

Dewatering Of Chlorella Through Carbon Dioxide Hydrate Formation For Biofuel Production, Charles Dow

Master's Theses

Renewable energy alternatives are currently of interest to meet ever-growing energy demands due to fossil fuel depletion and climate change. Biofuels, an alternative source, can be made from a range of organic matter. One option is algae, which can be grown without impacting food production and in a variety of conditions, however, the feasibility of converting algae into biofuel is of concern. Dewatering algae is a critical step in the process to make oil extraction more efficient. This study utilizes carbon dioxide clathrate hydrates as a novel process to dewater water saturated algae solutions. Clathrate hydrates are crystalline solids formed …

Methods To Increase Algae Biomass Productivity In Raceway Pond Monocultures, Ryan S. Anderson

Master's Theses

The economics of algae biofuels and bioproducts would be improved by increased biomass productivity. Two studies on this potential are described in this thesis – one on a locally isolated filamentous yellow-green alga and the other on a planktonic strain genetically improved via selective enrichment.

Polycultures have been viewed as productive, stable, and, in some cases, harvestable by natural bioflocculation. Local native strains might have higher productivity than culture collection strains because they are already adapted to local outdoor conditions. In this study, the filamentous yellow-green alga Tribonema minus was isolated from a local volunteer polyculture. Its productivity as a …

A Comparison Of The Army Corps Of Engineers Hydrologic Modeling System And Autodesk Storm And Sanitary Analysis For Hydraulic And Hydrologic Analysis And Design, Foster Josef Heifetz Campbell

Master's Theses

Hydrological and hydraulic effects of urban development is one of the key issues of study for improved water management. The addition of impervious surfaces to once pervious land and re-routing open channels and flow paths can cause flooding or declining water levels within a watershed. Many studies of these issues have found that there can be multiple factors causing hydrological and hydraulic impacts, and it can be hard to analyze and develop effective solutions without appropriate drainage software packages. However, there are multiple software packages available for use, and determining the correct one to use for a specific challenge can …

A Study On The Simultaneous Nitrification And Denitrification Process Of A Membrane Aerated Bioreactor Augmented By Biowish Aqua, Gavrielle Orman

Master's Theses

Nitrogen pollution is a growing problem that is detrimental to the environment and the economy. Traditional treatment of nitrogen is a multi-stage process, expensive, operationally intensive, and requires large land areas. This research studied the effects of BiOWiSH® Aqua (Aqua), a biological enhancement product, on the simultaneous nitrification and denitrification process in a membrane aerated bioreactor (MABR) to determine if it is a feasible application for wastewater treatment. The MABR used during experimentation was a small-scale batch reactor with a continuous flow of air through a silicone membrane.

The effect of carbon source and concentration on nitrogen removal rates and …

Integrated Aeration For Enhanced Nitrogen Conversion And Removal And The Effect Of Sampling Frequency On Productivity Estimates In Algae-Based Wastewater Treatment Systems, Lauren Parker

Master's Theses

Raceway ponds can be used to cultivate microalgae for purposes such as wastewater treatment and feedstock for animal feeds and biofuels. Besides nitrogen assimilation in biomass, nitrification can be a wastewater treatment mechanism but one that is hindered by the low nighttime dissolved oxygen concentrations (DO) common in wastewater treatment raceways. The large diel difference in pond conditions and changing influent wastewater quality also raises the question of the optimal frequency and timing of grab sample collection, to obtain representative water quality data. Three sets of triplicate pilot raceways (1,000-L, 0.30-m deep, fed primary clarifier effluent) were operated at an …

Carbonate Chemistry Characterization In A Low-Inflow Estuary With Recent Seagrass Loss, Jolie Higgins

Master's Theses

Estuaries are dynamic environments that are strongly affected by natural variability, as well as direct and indirect anthropogenic impacts. A better understanding of the drivers of carbon fluxes and biogeochemical variability in estuarine systems is needed, particularly with the increasing threat of ocean acidification. Morro Bay in Central California is a small nationally protected estuary, with seasonally low freshwater inputs. Since 2007, the bay has experienced a significant loss of native seagrass, Zostera marina, which is an important component of the marine ecosystem. Because seagrass photosynthesis decreases carbon dioxide and increases oxygen in the water column, the loss of seagrass …

Degradation Of Emerging Contaminants By Advanced Oxidation Using Multi-Walled Carbon Nanotubes And Continuous Ozone Injection, Emily N. Miller

Master's Theses

With a growing population and continuous accumulation of pollutants, water resources worldwide are quickly being depleted. Drastic improvements need to be made in both water conservation and treatment. Advanced oxidation processes (AOPs) have been developed to go above and beyond the capabilities of traditional wastewater treatment facilities to eliminate emerging contaminants from our water systems. AOPs increase the generation of hydroxyl radicals (•OH) in oxidation reactions, which are less selective and more reactive than other oxidants, such as ozone, so they are more effective at degrading persistent compounds. This study explored an AOP that utilizes ozonated multi-walled carbon nanotubes (MWCNTs) …

Investigating The Rapid Curing Possibility Of Geopolymer Concrete, Ashlesh Banjara

Master's Theses

Recent studies of heat-cured fly ash based green geopolymer concrete have shown its suitability for fabrication of structural members. Fabrication of these structural members requires continuous moderate heating (145°F) for 24 hrs, and an oven essential, for a large member can quickly turn into an energy guzzler, potentially eliminating the green credentials of the product. The proposed research involves the development of a frontal polymerization (FP) method that achieves rapid curing of geopolymer at ambient condition after short-term heat application. Initial work shows thermal FP in geopolymer is a possibility and might be a solution to the problem. The process …

Effects On Strength And Corrosive Characteristics Of Hardened Cement Paste Using Rice Husk And Sugarcane Bagasse, Travis D. Garrett

Master's Theses

Cement is one of the most used building materials in the world. The production of ordinary Portland cement contributes to the release of harmful greenhouse gasses. In an effort to reduce these emissions, different materials are being evaluated for their use as partial replacements for ordinary Portland cement. Rice husk and sugarcane bagasse are agricultural waste products and large amounts of these are currently landfilled, making them inexpensive and available. When combusted at high temperatures they form rice husk ash and sugarcane bagasse ash that have pozzolanic characteristics. The high silicon dioxide in these products makes them good options to …

Macrobenthos Responses To Dam Removal And Habitat Restoration In The West River, Connecticut, Robert W. Sheridan

Master's Theses

Dam removal is being increasingly used nationwide to restore impaired rivers and streams. While dam removals are becoming more prevalent, little is known about whether these efforts create conditions for the enhancement and or establishment of desired biota.

Ongoing monitoring of these projects is an important step in the restoration process to ensure project goals are being met and the restoration has been successful.

This thesis focuses on the Pond Lily Dam removal and restoration which took place in October 2015 along the West River in New Haven Connecticut that removed an aging mill dam with the objective of restoring …

Effect Of Bio-Augmentation Product Biowish® Septic Rescue On The Wastewater Treatment Performance Of Residential Septic Tanks, Kimberly Michelle Lamar Merilles

Master's Theses

Residential septic systems provide reliable wastewater treatment for over 26 million homes and facilities in the United States, and many more worldwide. When properly maintained, these systems are reliable, low-cost, and long-term treatments for residential wastewater. When neglected, septic systems can fail and lead to health concerns and ecological harm to soil and groundwater contamination through the improperly treated wastewater effluent.

This study tested the effect of the bio-augmentation product BiOWiSH® Septic Rescue of BiOWiSH® Technologies International, Inc. (hereafter referred to as BiOWiSH) on the biological treatment of residential septic tanks. BiOWiSH is meant to act as a bio-augmentation product …

Indoor Air Monitoring Of Ethanol And Benzene In A Pilot Winery Using Active Sampling, Andrew I. Kaneda

Master's Theses

Acute indoor concentrations of benzene and ethanol were evaluated in the California Polytechnic State University San Luis Obispo’s pilot winery workroom. Air samples were collected during four different wine-making activities: fermentation, fermentation with Brix content testing, post-alcoholic fermentation pressing, and storage/finishing. Average workroom benzene concentrations ranged from 0.05 to 0.12 mg/m³. Ethanol concentrations in the winery workroom varied with the activity, ranging from 0.9 to 12 mg/m³. Pressing and fermentation with Brix content testing both led to higher indoor ethanol concentrations than fermentation without Brix content testing and storage/finishing.

Tracer gas decay air exchange tests were …

Wastewater Treatment And Nitrogen Removal In Bench-Scale Photobioreactors Operated With Solids Recycling, Erik Hoffnagle

Master's Theses

This thesis examines the effect of solids recycling on nitrification, organic carbon removal, and algal-bacterial productivity in bench-scale photobioreactors (PBRs) simulating winter pond conditions in San Luis Obispo, California and a 6-acre raceway system in Delhi, California. Two sets of duplicate photobioreactors operated with or without solids recycling were fed primary clarifier effluent (1^oEff) for the first experiment, and facultative pond effluent (FAC) for the second experiment.

In both experiments mean productivity was lower in solids recycled PBRs (3.67 and 2.3 g/m²-day), than in controls without solids recycling (4.15 and 3.9 g/m²-day). When fed …

Midpoint And Endpoint Sustainability Assessment Of U.S. And China Manufacturing: A Comparative Mrio+Recipe Analysis, Mustafa Saber

Master's Theses

Manufacturing is among the most important industries for an economy, which creates value-added, fosters innovation, stimulates employment and economic growth. Therefore, manufacturing industries are crucial for a country’s sustainable development not only for economic reasons but also for social (.e.g. employment, tax, etc.) and environmental ones. Thus, manufacturing activities’ contribution to the economy is critically related with environmental and social impacts. Sustainable economic growth is essential and necessary for a country to provide all necessary goods and services to its growing population.

And, this is highly linke with the creation of new jobs and in this context, manufacturing jobs have …

Global Renewable And Nonrenewable Energy Use Impact Assessment Of U.S. Manufacturing: An Integrated Cradle-To-Gate Lca And Dea Approach, Bahadir Ezici

Master's Theses

In this thesis, U.S. manufacturing industries` global supply chain-linked energy use and economic output are investigated considering a total of 16 renewable and nonrenewable energy carriers. A multiregion input output (MRIO) framework is employed to conduct the global supply chain-linked energy use impact assessment. The study period was between 1995 and 2014 based on data availability. Thus, 20 MRIO models were developed. Each MRIO model consists of the 40 largest economies of the world and the rest of the world (ROW) as the 41st country. Each country’s economy was structured into 35 manufacturing and service industries based on the Worth …

Increasing Algal Productivity And Treatment Potential In Raceways Fed Clarified Municipal Wastewater, Christopher D. Pittner

Master's Theses

Two sets of triplicate pilot algal raceway ponds (1000-L, 0.30-m deep, paddle wheel mixed) were operated for 14 months at a California wastewater treatment plant to treat wastewater and generate algal-bacterial biomass as biofuel feedstock. Two experiments were run to determine the effect on biomass productivity of (1) hydraulic residence time (HRT: 2, 3, 4, or 4.5 days) and (2) feeding schedule (18 small pulses during 8 AM-4 PM [diurnal] versus 20 large pulses during 4 AM-12 AM [diel]). The target productivity was at least 20 g volatile suspended solids per m2 of pond per day. Additional output variables were …

Design And Analysis Of A Parabolic Trough Solar Concentrator, George N. Skouras

Master's Theses

A prototype solar desalination system (SODESAL) with a parabolic-trough solar concentrator (PTSC) and evacuated tube was designed and analyzed to determine the solar thermal capabilities for small-scale distillation and energy generation. A proof-of-concept study verified that distillation is possible with the system as designed, however a rupture occurred in the copper heat-pipe heat exchanger due to overheating. The internal temperatures of an aluminum heat transfer fin were measured inside an evacuated tube typically used in solar water heater systems to understand the lateral heat distribution and identify possible causes of the rupture. Solar radiation was measured for both the summer …

The Effect Of Destoning And Enzymatic Pretreatments On The Biofuel Production From Olive Cake, Patrick Tai

Master's Theses

More than 16,000 tons of olive cake was produced in the United States in 2017. Olive cake is a by-product of olive oil extraction, which has limited animal feed potential, and poses an environmental threat when landfilled due to its high organic load and polyphenol content. This residue has potential for biofuel (bioethanol and biomethane) production because it is rich in polysaccharides such as pectin, hemicellulose, and cellulose. Yet, olive cake contains olive stones that can impede its conversion to biofuel. Therefore, two methods of destoning, centrifugation and screening by horizontal screw press, were first compared. Both methods removed an …

Anaerobic Digestion Of Wastewater-Grown Algae Biomass With Optimized Biogas Yields And Nutrient Solubilzation, Tiffany A. Racz

Master's Theses

Whole-cell algae biomass grown on nutrient-rich wastewater can be anaerobically digested to produce renewable heat and power, and to solubilize nutrients to grow additional algae biomass in a biorefinery system. In this study, algae biomass was grown on clarified primary wastewater in 33-m² ponds at a 4-day residence time with sedimentation harvesting of the biomass. Lab digesters were used to test biogas yields and nutrient solubilization from the anaerobic digestion of algal biomass with and without mixing and with sonication as a pretreatment. Additionally, algae were fed to unheated and unmixed 1135-L pilot digesters to determine the effects of …

Study Of The Effect Of Biowish Aqua On Simultaneous Nitrification And Denitrification In A Membrane Aerated Bioreactor, Joelle Arakaki

Master's Theses

This research entails the investigation of the effects of a bioaugmentation product from BiOWiSH^® called Aqua, referred to as “Aqua” for the remainder of this paper, on the nitrogen removal rate in a membrane aerated bioreactor (MABR). This research was conducted using a MABR design that consisted of a silicone membrane and continuous flow airline with compressed air. The membrane system was designed to supply oxygen, creating an aerated layer at the membrane-biofilm interface and an anoxic layer at the biofilm-water interface. Laboratory experiments were conducted to compare the nitrogen removal rates of natural bacteria alone to natural bacteria …

Characterization And Removal Efficiency Comparison Of Bioretention Soil Media Mixtures, Nathan T. Hanson

Master's Theses

Bioretention cells have become a commonly used green infrastructure technique to help infiltrate and remove contaminants from stormwater runoff. Bioretention cells are constructed from a layered or heterogeneous soil mixture designed to optimize their ability to infiltrate influent stormwater and remove contaminants carried by the water as it filters through the soil media. The soil mixture, composition, and planting vary depending the local regulatory agencies. As urbanization occurs across the United States, more natural land is converted from pervious surfaces, such as grasslands and forests, to impervious surfaces such as asphalt and concrete, to help reduce the impact of the …