Environmental Engineering Commons^™

Alginate/Silica Hybrid Materials For Immobilization Of Green Microalgae Chlorella Vulgaris For Cellbased Sensor Arrays, Angela K. Pannier, Ulrich Soltmann, Bettina Soltmann, Rolf Altenburger, Mechthild Schmitt-Jansen

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Thin layers and patterned dot arrays of sodium alginate containing living microalgal cells were deposited onto glass carriers which were subsequently gelled using amino-functionalized silica sol to obtain reinforced alginate hydrogels. The resulting alginate/silica hybrid materials showed improved stability in salt-containing solutions compared to alginate gels gelled by traditional methods using Ca²⁺-ions. Cell arrays were patterned by printing nanolitre-scale drops of sodium alginate/cell suspension using a noncontact micro-dosage system which allows the printing of solutions of high viscosity. Cultures of the green microalga Chlorella vulgaris were immobilized within the newly developed alginate/silica hydrogels in order to demonstrate the …

In Vitro Development Of Preimplantation Porcine Embryos Using Alginate Hydrogels As A Three-Dimensional Extracellular Matrix, Catherine Sargus-Patino, Elane C. Wright, Sarah A. Plautz, Jeremy R. Miles, Jeff L. Vallet, Angela K. Pannier

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Between Days 10 and 12 of gestation, porcine embryos undergo a dramatic morphological change, known as elongation, with a corresponding increase in oestrogen production that triggers maternal recognition of pregnancy. Elongation deficiencies contribute to embryonic loss, but exact mechanisms of elongation are poorly understood due to the lack of an effective in vitro culture system. Our objective was to use alginate hydrogels as three-dimensional scaffolds that can mechanically support the in vitro development of preimplantation porcine embryos. White cross-bred gilts were bred at oestrus (Day 0) to Duroc boars and embryos were recovered on Days 9, 10 or 11 of …

Nutrient Concentrations Of Runoff As Affected By The Diameter Of Unconsolidated Material From Feedlot Surfaces, John E. Gilley, Gregory Boone, David B. Marx

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Beef cattle feedlots contain unconsolidated material that accumulates on the feedlot surface during a feeding cycle. This study was conducted to measure the effects of varying diameters of unconsolidated surface material and varying flow rates on nutrient concentrations in runoff. Unconsolidated surface material with an average diameter of 4.76, 9.53, 19.1, or 47.5 mm and a composite sample with a 15.2 mm mean diameter were placed within 0.75 m wide × 4.0 m long plot areas. Flow was then introduced at the top of the plots in successive increments, and runoff samples for water quality analyses were obtained. Particle diameter …

Dynamic Analysis Of Dna Nanoparticle Immobilization To Model Biomaterial Substrates Using Combinatorial Spectroscopic Ellipsometry And Quartz Crystal Microbalance With Dissipation, Tadas Kasputis, Alex Pieper, Mathias Schubert, Angela K. Pannier

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Gene expression within cells can be altered through gene delivery approaches, which have tremendous potential for gene therapy, tissue engineering, and diagnostics. Substrate-mediated gene delivery describes the delivery of plasmid DNA or DNA complexed with nonviral vectors to cells from a surface, with the DNA immobilized to a substrate through specific or nonspecific interactions. In this work, DNA-nanoparticle (DNA–NP) adsorption to substrates is evaluated using combinatorial, in situ spectroscopic ellipsometry and quartz crystal microbalance with dissipation (SE/QCM-D), to evaluate the basic dynamic processes involved in the adsorption and immobilization of DNA–NP complexes to substrates. The concentration of DNA–NP solutions influences …

Soybean Yield, Evapotranspiration, Water Productivity, And Soil Water Extraction Response To Subsurface Drip Irrigation And Fertigation, Suat Irmak, James E. Specht, Lameck O. Odhiambo, J. M. Rees, K. G. Cassman

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Soybean [Glycine max (L.) Merr.] yield, irrigation water use efficiency (IWUE), crop water use efficiency (CWUE), evapotranspiration water use efficiency (ETWUE), and soil water extraction response to eleven treatments of full, limited, or delayed irrigation versus a rainfed control were investigated using a subsurface drip irrigation (SDI) system at a research site in south-central Nebraska. The SDI system laterals were 0.40 m deep in every other row middle of 0.76 m spaced plant rows. Actual evapotranspiration (ET_a) was quantified in all treatments and used to schedule irrigation events on a 100% ET_a replacement basis in all but …

Combined Qcm-D/Ge As A Tool To Characterize Stimuli-Responsive Swelling Of And Protein Adsorption On Polymer Brushes Grafted Onto 3d-Nanostructures, Meike Koenig, Tadas Kasputis, Daniel Schmidt, Keith B. Rodenhausen Jr., Klaus-Jochen Eichhorn, Angela K. Pannier, Mathias Schubert, Manfred Stamm, Petra Uhlmann

Department of Agricultural and Biological Systems Engineering: Faculty Publications

A combined setup of quartz crystal microbalance and generalized ellipsometry can be used to comprehensively investigate complex functional coatings comprising stimuli-responsive polymer brushes and 3D nanostructures in a dynamic, noninvasive in situ measurement. While the quartz crystal microbalance detects the overall change in areal mass, for instance, during a swelling or adsorption process, the generalized ellipsometry data can be evaluated in terms of a layered model to distinguish between processes occurring within the intercolumnar space or on top of the anisotropic nanocolumns. Silicon films with anisotropic nanocolumnar morphology were prepared by the glancing angle deposition technique and further functionalized by …

Parallel Synthesis Of Poly(Amino Ether)-Templated Plasmonic Nanoparticles For Transgene Delivery, James Ramos, Thrimoorthy Potta, Olivia Scheideler, Kaushal Rege

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Plasmonic nanoparticles have been increasingly investigated for numerous applications in medicine, sensing, and catalysis. In particular, gold nanoparticles have been investigated for separations, sensing, drug/nucleic acid delivery, and bioimaging. In addition, silver nanoparticles demonstrate antibacterial activity, resulting in potential application in treatments against microbial infections, burns, diabetic skin ulcers, and medical devices. Here, we describe the facile, parallel synthesis of both gold and silver nanoparticles using a small set of poly(amino ethers), or PAEs, derived from linear polyamines, under ambient conditions and in absence of additional reagents. The kinetics of nanoparticle formation were dependent on PAE concentration and chemical composition. …

Surface-Enhanced Raman Spectral Biomarkers Correlate With Ankle Brachial Index And Characterize Leg Muscle Biochemical Composition Of Patients With Peripheral Arterial Disease, Kim Cluff, Abby M. Kelly, Panagiotis Koutakis, Xiang N. He, Xi Huang, Yongfeng Lu, Iraklis I. Pipinos, George P. Casale, Jeyamkondan Subbiah

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Peripheral arterial disease (PAD) is characterized by atherosclerotic blockages of the arteries supplying the lower extremities, which cause a progressive accumulation of ischemic injury to the skeletal muscles of the lower limbs. This injury includes altered metabolic processes, damaged organelles, and compromised bioenergetics in the affected muscles. The objective of this study was to explore the association of Raman spectral signatures of muscle biochemistry with the severity of atherosclerosis in the legs as determined by the Ankle Brachial Index (ABI) and clinical presentation. We collected muscle biopsies from the gastrocnemius (calf muscle) of five patients with clinically diagnosed claudication, five …

Water-Stable Three-Dimensional Ultrafine Fibrous Scaffolds From Keratin For Cartilage Tissue Engineering, Helan Xu, Shaobo Cai, Lan Xu, Yiqi Yang

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Intrinsically water-stable scaffolds composed of ultrafine keratin fibers oriented randomly and evenly in three dimensions were electrospun for cartilage tissue engineering. Keratin has been recognized as a biomaterial that could substantially support the growth and development of multiple cell lines. Besides, three-dimensional (3D) ultrafine fibrous structures were preferred in tissue engineering due to their structural similarity to native extracellular matrices in soft tissues. Recently, we have developed a nontraditional approach to developing 3D fibrous scaffolds from alcohol-soluble corn protein, zein, and verified their structural advantages in tissue engineering. However, keratin with highly cross-linked molecular structures could not be readily dissolved …

Intrinsically Water-Stable Keratin Nanoparticles And Their In Vivo Biodistribution For Targeted Delivery, Helan Xu, Zhen Shi, Narendra Reddy, Yiqi Yang

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Highly water-stable nanoparticles of around 70 nm and capable of distributing with high uptake in certain organs of mice were developed from feather keratin. Nanoparticles could provide novel veterinary diagnostics and therapeutics to boost efficiency in identification and treatment of livestock diseases to improve protein supply and ensure safety and quality of food. Nanoparticles could penetrate easily into cells and small capillaries, surpass detection of the immune system, and reach targeted organs because of their nanoscale sizes. Proteins with positive and negative charges and hydrophobic domains enable loading of various types of drugs and, hence, are advantageous over synthetic polymers …

Long-Term Activity Of Biohybrid Coatings Of Atrazine-Degrading Bacteria Pseudomonas Sp. Adp, Angela K. Pannier, T. Lehrer, M. Vogel, U. Soltmann, H. Bottcher, S. Tarre, M. Green, J. Raff, K. Pollmann

Department of Agricultural and Biological Systems Engineering: Faculty Publications

The atrazine-degrading bacterial strain Pseudomonas sp. ADP was immobilized by the sol–gel process within thin silica layers coated onto water-retaining carrier materials (expanded clay pellets and scoria). The performance of the obtained biohybrid material has been investigated concerning long-term activity under non-growth conditions. Experiments were run in phosphate buffer containing atrazine (20 mg l^-1) as the sole source of carbon and nitrogen. Even after one year of consecutive batch tests, P. ADP immobilized onto expanded clay pellets showed a high atrazine degradation activity. In the course of long-term batch experiments, the average amount of removed atrazine was about …

Use Of Controller Area Network (Can) Data To Determine Field Efficiencies Of Agricultural Machinery, Santosh Pitla, N. Lin, Joe D. Luck, Scott A. Shearer

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Controller Area Network (CAN) bus is used on current agricultural equipment for in-vehicle communication among multiple microcontrollers. In this study, CAN data was collected from the tractor diagnostic port during three field operations: anhydrous ammonia (NH₃) application, field cultivation, and planting. Specifically, CAN messages corresponding to engine fuel use rate were filtered, decoded, and analyzed. High fuel use rates were recorded from the tractor when implements were engaged in the soil compared to when the implements were lifted out of the ground at the end of the passes or, when the tractor was turning and idling. Threshold fuel …

Narrow Grass Hedge Effects On The Transport Of Antimicrobials And Antimicrobial Resistance Genes Following Land Application Of Swine Slurry, Bhavneet Soni

Department of Civil and Environmental Engineering: Dissertations, Theses, and Student Research

The objective of this study was to determine the effects of manure amendment and narrow grass hedges on the fate and transport of antimicrobials and ARGs in runoff and in soil following the land application of swine manure slurry. Swine manure slurry was land applied to 0.75m wide by 4.0m long plots established on an Aksarben silty clay loam soil located in southeast Nebraska. The treatment factor manure amendment consisted of two levels: no manure application and manure application to meet the 3 year nitrogen (N) requirements for corn. The treatment factor of grass hedge was established for half of …

Drainage Structure Datasets And Effects On Lidar-Derived Surface Flow Modeling, Ruopu Li, Zhenghong Tang, Xu Li, Jessie Winter

School of Natural Resources: Faculty Publications

With extraordinary resolution and accuracy, Light Detection and Ranging (LiDAR)-derived digital elevation models (DEMs) have been increasingly used for watershed analyses and modeling by hydrologists, planners and engineers. Such high-accuracy DEMs have demonstrated their effectiveness in delineating watershed and drainage patterns at fine scales in low-relief terrains. However, these high-resolution datasets are usually only available as topographic DEMs rather than hydrologic DEMs, presenting greater land roughness that can affect natural flow accumulation. Specifically, locations of drainage structures such as road culverts and bridges were simulated as barriers to the passage of drainage. This paper proposed a geospatial method for producing …

Elementary Implantable Force Sensor For Smart Orthopaedic Implants, Rebecca A. Wachs, David Ellstein, John Drazan, Colleen P. Healey, Richard L. Uhl, Kenneth A. Connor, Eric H. Ledet

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Implementing implantable sensors which are robust enough to maintain long term functionality inside the body remains a significant challenge. The ideal implantable sensing system is one which is simple and robust; free from batteries, telemetry, and complex electronics. We have developed an elementary implantable sensor for orthopaedic smart implants. The sensor requires no telemetry and no batteries to communicate wirelessly. It has no on-board signal conditioning electronics. The sensor itself has no electrical connections and thus does not require a hermetic package. The sensor is an elementary L-C resonator which can function as a simple force transducer by using a …

Development Of A Field Test For Total Suspended Solids Analysis, Jessica Branigan

Department of Civil and Environmental Engineering: Dissertations, Theses, and Student Research

Total suspended solids (TSS) are all particles in water that will not pass through a glass fiber filter with a pore size less than 2 μm, including sediments, algae, nutrients, and metals. TSS is an important water quality parameter because of its adverse effects on aquatic species and wildlife. The EPA has proposed a regulation for turbidity, a related water quality parameter, which has been stayed pending further testing. TSS is regulated through the EPA via the NPDES in many states. Since there are no accepted field tests for TSS, projects with TSS regulations must send samples to a laboratory …

Improving The Sweeping Efficiency Of Permanganate Into Low Permeable Zones To Treat Tce: Experimental Results And Model Development, Chanat Chokejaroenrat, Negin Kananizadeh, Chainarong Sakulthaew, Steve D. Comfort, Yusong Li

Nebraska Water Center: Faculty Publications

The residual buildup and treatment of dissolved contaminants in low permeable zones (LPZs) is a particularly challenging issue for injection-based remedial treatments. Our objective was to improve the sweeping efficiency of permanganate into LPZs to treat dissolved-phase TCE. This was accomplished by conducting transport experiments that quantified the ability of xanthan-MnO₄⁻ solutions to penetrate and cover (i.e., sweep) an LPZ that was surrounded by transmissive sands. By incorporating the non-Newtonian fluid xanthan with MnO₄⁻, penetration of MnO₄⁻ into the LPZ improved dramatically and sweeping efficiency reached 100% in fewer pore volumes. To quantify …

Passive Stormwater Samplers For Sampling Highway Runoff From Bmps: Feasibility Studies, Mitchell R. Klein

Department of Civil and Environmental Engineering: Dissertations, Theses, and Student Research

Pollution from highway stormwater runoff has been an increasing area of concern within the environmental field. To respond to the need for reduced contamination within runoff many Best Management Practices (BMPs) have been implemented. One difficult aspect of BMPs is monitoring their effectiveness along with determining effluent concentrations. The current methods for stormwater sampling include sending technicians or installing an auto-sampler to collect either grab or composite samples. These methods become costly, cumbersome and infeasible due to the potentially large amount of BMPs across a region and the irregularity and difficulty of predicting storms. Passive samplers have proven themselves as …

Laboratory Evaluation Of A Turn Compensation Control System For A Ground Sprayer, Wesley M. Porter, Jorge A. Rascon, Yeyin Shi, Randal K. Taylor, Paul A. Weckler

Department of Agricultural and Biological Systems Engineering: Faculty Publications

The ability to compensate sprayer nozzle flow across a horizontal boom has the potential to mitigate the problem of inaccurate chemical application rate due to lateral speed differences when sprayers are turning. A laboratory testing platform and procedure were developed and tested with a commercial turn compensation control system for a ground sprayer. Virtual paths consisting of simulated GPS signals representing right and left turns separated by straight segments were simulated with four turning radii (75%, 125%, 250%, and 500%) based on boom width. Actual application rates from 11 nozzles spread across the boom were measured using load cells in …

Preliminary Analysis Of Ghg Production From The Lincoln Water System To Determine Operating Energy And Infrastructure Construction Impacts, Martin Gakuria

Department of Environmental Engineering: Theses and Other Student Research

The Lincoln Water System (LWS) provides water to the citizens, industries, and commercial areas within and near the City of Lincoln, Nebraska. The intent of this thesis is to determine if reductions in future per capita water demand will help reduce the building of infrastructure and reduce future pumping costs and uses the computation of greenhouse gasses to compare the effects of different degrees of water conservation.

Data analysis was performed on water production statistics and data (1994-2011) collected and provided by the City of Lincoln, Nebraska. Projections in accordance with the Lincoln-Lancaster County 2040 Comprehensive Plan were made to …

Stormwater Pollution Treatment Bmp Discharge Structures, Miles F. Simmons

Department of Civil and Environmental Engineering: Dissertations, Theses, and Student Research

Structural best management practices (BMPs) are used to capture and treat stormwater runoff. Most structural BMPs provide treatment by filtering runoff through a filter media or collecting it in a detention basin and slowly discharging it over an extended period of time to allow suspended solids and associated contaminants to settle out. The purpose of this study is to design an effective outlet structure that provides adequate filtration or slows discharge to 40 hours.

A model detention basin was constructed in the Civil Engineering Hydraulics Laboratory at the University of Nebraska-Lincoln (UNL) and two full scale outlet structures were tested …

Soil Organic Carbon Dynamics In Agriculture: Model Development And Application From Daily To Decadal Timescales, Matthew P. Pelton

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

Soil carbon (C) is the largest terrestrial C pool globally, containing more C than the atmosphere and biosphere. Soil organic carbon (SOC) is a source and sink of CO₂ emissions to and from the atmosphere, thus influencing future climate change. Understanding SOC dynamics is also important for maintaining C stocks to sustain and improve crop yields. An existing model to estimate changes in SOC due to respiration was modified to operate in three computational platforms: MS Excel, MS Excel with Visual Basic for Applications, and supercomputing. This model was validated against CO₂ flux data from a 9-year field …

Predicting Groundwater Trading Participation In The Upper Republican River Natural Resource District, Elizabeth M. Juchems

Department of Agricultural Economics: Dissertations, Theses, and Student Research

The goal of this thesis is to predict participation in groundwater trading and the directions of trades among participants. Specifically, the paper considers both formal and informal trading of groundwater used for crop irrigation purposes and attempts to identify those characteristics that predict the probability of trade participation and whether an individual is a buyer or seller of groundwater rights. While the public benefits from efficient use of groundwater include adequate stream flow in hydrologically connected areas and future use of groundwater supplies, there are significant private benefits to landowners especially in water-short areas. Groundwater trading can help move water …

Building Upon Common-Pool Resource Theory To Explore Success In Transitioning Water Management Institutions, Christina M. Hoffman

School of Natural Resources: Dissertations, Theses, and Student Research

Nebraska, like many regions around the world, is faced with the challenge of adapting to a new era in water management. Increasing demands for water resources, mounting concerns over threatened and endangered species, and obligations to abide by interstate water allocation agreements have motivated Nebraska to revisit traditional water management approaches. However, although Nebraska’s water management institutions have undergone much change, little research exists on the influence these changes have had on the ability of water institutions to successfully manage water allocations. This research (1) qualitatively explores the perspectives and experiences of stakeholders in the overappropriated region of the Platte …

2013 Nebraska Water Monitoring Programs Report, Marty Link, Joe Kenning

Nebraska Department of Environmental Quality: Reports

The Nebraska Department of Environmental Quality (NDEQ) is charged with monitoring, assessing, and to the extent possible, managing the state’s water resources. The purpose of this work is to protect and maintain good quality water and encourage or execute activities to improve poor water quality. Monitoring is done on the over 16,000 miles of flowing rivers and streams, our greater than 148,000 acres of surface water in lakes and reservoirs, and the vast storage of groundwater in Nebraska’s aquifers.

Evapotranspiration And Surface Energy Balance Of A Common Reed-Dominated Riparian System In The Platte River Basin, Central Nebraska, Isa Kabenge, Suat Irmak, John E. Gilley, George E. Meyer, Stevan Z. Knezevic, Timothy J. Arkebauer, Duane Woodward, Milton Moravek

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Quantifying actual evapotranspiration (ET_a) of riparian zones is important for more robust water balance analyses that will enable better planning, managing, and allocating of water resources as well as developing strategies to protect delicate riparian ecosystem functions. The ET_a, sensible heat flux (H), net radiation (R_n), soil heat flux (G), meteorological variables (air temperature, T_a; incoming shortwave radiation, R_s; wind speed, u₃; relative humidity, RH; vapor pressure deficit, VPD; precipitation, etc.), and albedo were measured on an hourly time step, and leaf area index (LAI) and plant height …

Variability Of Extreme Precipitation In Coastal River Basins Of The Southern Mexican Pacific Region, Nancy Perez-Morga, Thomas Kretzschmar, Tereza Cavazos, Stephen V. Smith, Francisco Munoz-Arriola

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Extreme wet and dry years (± 1 standard deviation, respectively), as well as the top 95 percentile (P95) of daily precipitation events, derived from tropical cyclone (TC) and nontropical cyclone (NTC) rainfall, were analyzed in coastal river basins in Southern Oaxaca, Mexico (Río Verde, Río Tehuantepec, and the Southern Coast). The study is based on daily precipitation records from 47 quality-controlled stations for the 1961 to 1990 period and TC data for the Eastern Tropical Pacific (EPAC). The aim of this study was to evaluate extreme (dry and wet) trends in the annual contribution of daily P95 precipitation events and …

2013 Nebraska Groundwater Quality Monitoring Report

Nebraska Department of Environmental Quality: Reports

The 2001 Nebraska Legislature passed LB329 (Neb. Rev. Stat. §46-1304) which, in part, directed the Nebraska Department of Environmental Quality (NDEQ) to report on groundwater quality monitoring in Nebraska. Reports have been issued annually since December 2001. The text of the statute applicable to this report follows: “The Department of Environmental Quality shall prepare a report outlining the extent of ground water quality monitoring conducted by natural resources districts during the preceding calendar year. The department shall analyze the data collected for the purpose of determining whether or not ground water quality is degrading or improving and shall present the …

Use Of A Surrogate To Evaluate The Impact Of Tillage On The Transport Of Steroid Hormones From Manure-Amended Agricultural Fields, Sagor Biswas, William L. Kranz, Charles A. Shapiro, Mitiku Mamo, Shannon L. Bartelt-Hunt, Daniel D. Snow, David P. Shelton, David D. Tarkalson, Terry L. Mader, Simon J. Van Donk, Tian C. Zhang

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Beef feedlot manure distributed to row crop production areas is a potential surface water contaminant source of the steroid hormones commonly used in beef cattle production. This article reports on research conducted at the University of Nebraska Haskell Agricultural Laboratory near Concord, Nebraska, in July 2009. Manure, collected from beef feedlot pens, was stockpiled for ten months prior to application to a row crop field. Previous research identified that the detection frequency of steroid hormones in beef manure varies greatly. Thus, a surrogate (17α-ethynylestradiol, EE2) was applied at a rate of 75 g ha^-1 to ensure detectable concentrations in …

Runoff Nutrient Transport As Affected By Land Application Method, Swine Growth Stage, And Runoff Rate, J. E. Gilley, Shannon L. Bartelt-Hunt, S. J. Lamb, X. Li, D. B. Marx, D. D. Snow, D. B. Parker, B. L. Woodbury

Department of Agricultural and Biological Systems Engineering: Faculty Publications

This study was conducted to measure the effects of slurry application method, swine growth stage, and flow rate on runoff nutrient transport. Swine slurry was obtained from production units containing grower pigs, finisher pigs, or sows and gilts. The swine slurry was applied using broadcast, disk, or injection methods at a rate required to meet annual nitrogen requirements for corn. Three 30 min simulated rainfall events, separated by 24 h intervals, were applied to the experimental plots at an intensity of 70 mm h^-1. Inflow was applied at the top of each plot in four successive increments after …